Isolated polynucleotides and polypeptides, and methods of using same for increasing nitrogen use efficiency, yield, growth rate, vigor, biomass, oil content, and/or abiotic stress tolerance

ABSTRACT

Provided are isolated polynucleotides and nucleic acid constructs which comprise a nucleic acid sequence at least 80% identical to a nucleic acid sequence selected form the group consisting of SEQ ID NOs: 277, 1-276, 278-469 and 785-2397; and isolated polypeptides which comprise an amino acid sequence at least 80% homologous to an amino acid sequence selected from the group consisting of SEQ ID NOs: 482, 470-481, 483-784 and 2398-3818. Also provided are transgenic cells and plants expressing same and methods of using same for increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or abiotic stress tolerance of a plant.

RELATED APPLICATIONS

This application is a division of U.S. patent application Ser. No.13/819,777 filed on Feb. 28, 2013, which is a National Phase of PCTPatent Application No. PCT/IB2011/053697 having International FilingDate of Aug. 23, 2011, which claims the benefit of priority under 35 USC§ 119(e) of U.S. Provisional Patent Application Nos. 61/437,715 filed onJan. 31, 2011, 61/405,260 filed on Oct. 21, 2010 and 61/378,003 filed onAug. 30, 2010. This PCT Patent Application No. PCT/M2011/053697 is alsoa Continuation-In-Part (CIP) of PCT Patent Application No.PCT/M2011/051843 having International Filing Date of Apr. 27, 2011. Thecontents of the above applications are all incorporated by reference asif fully set forth herein in their entirety.

SEQUENCE LISTING STATEMENT

The ASCII file, entitled 78704SequenceListing.txt, created on Aug. 26,2019, comprising 9,648,108 bytes, submitted concurrently with the filingof this application is incorporated herein by reference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to novelpolynucleotides and polypeptides which can increase nitrogen useefficiency, fertilizer use efficiency, yield (e.g., seed/grain yield,oil yield), growth rate, vigor, biomass, oil content, fiber yield, fiberquality and/or length, abiotic stress tolerance and/or water useefficiency of a plant.

A common approach to promote plant growth has been, and continues to be,the use of natural as well as synthetic nutrients (fertilizers). Thus,fertilizers are the fuel behind the “green revolution”, directlyresponsible for the exceptional increase in crop yields during the last40 years, and are considered the number one overhead expense inagriculture. Of the three macronutrients provided as main fertilizers[Nitrogen (N), Phosphate (P) and Potassium (K)], nitrogen is often therate-limiting element in plant growth and all field crops have afundamental dependence on inorganic nitrogenous fertilizer. Nitrogenusually needs to be replenished every year, particularly for cereals,which comprise more than half of the cultivated areas worldwide. Forexample, inorganic nitrogenous fertilizers such as ammonium nitrate,potassium nitrate, or urea, typically accounts for about 40% of thecosts associated with crops such as corn and wheat.

Nitrogen is an essential macronutrient for the plant, responsible forbiosynthesis of amino and nucleic acids, prosthetic groups, planthormones, plant chemical defenses, and the like. In addition, nitrogenis often the rate-limiting element in plant growth and all field cropshave a fundamental dependence on inorganic nitrogen. Thus, nitrogen istranslocated to the shoot, where it is stored in the leaves and stalkduring the rapid step of plant development and up until flowering. Incorn for example, plants accumulate the bulk of their organic nitrogenduring the period of grain germination, and until flowering. Oncefertilization of the plant has occurred, grains begin to form and becomethe main sink of plant nitrogen. The stored nitrogen can be thenredistributed from the leaves and stalk that served as storagecompartments until grain formation.

Since fertilizer is rapidly depleted from most soil types, it must besupplied to growing crops two or three times during the growing season.In addition, the low nitrogen use efficiency (NUE) of the main crops(e.g., in the range of only 30-70%) negatively affects the inputexpenses for the farmer, due to the excess fertilizer applied. Moreover,the over and inefficient use of fertilizers are major factorsresponsible for environmental problems such as eutrophication ofgroundwater, lakes, rivers and seas, nitrate pollution in drinking waterwhich can cause methemoglobinemia, phosphate pollution, atmosphericpollution and the like. However, in spite of the negative impact offertilizers on the environment, and the limits on fertilizer use, whichhave been legislated in several countries, the use of fertilizers isexpected to increase in order to support food and fiber production forrapid population growth on limited land resources. For example, it hasbeen estimated that by 2050, more than 150 million tons of nitrogenousfertilizer will be used worldwide annually.

Increased use efficiency of nitrogen by plants should enable crops to becultivated with lower fertilizer input, or alternatively to becultivated on soils of poorer quality and would therefore havesignificant economic impact in both developed and developingagricultural systems.

Genetic improvement of fertilizer use efficiency (FUE) in plants can begenerated either via traditional breeding or via genetic engineering.

Attempts to generate plants with increased FUE have been described inU.S. Pat. Appl. No. 20020046419 to Choo, et al.; U.S. Pat. Appl. No.2005010879 to Edgerton et al.; U.S. Pat. Appl. No. 20060179511 to Chometet al.; Good, A, et al. 2007 (Engineering nitrogen use efficiency withalanine aminotransferase. Canadian Journal of Botany 85: 252-262); andGood A G et al. 2004 (Trends Plant Sci. 9:597-605).

Yanagisawa et al. (Proc. Natl. Acad. Sci. U.S.A. 2004 101:7833-8)describe Dof1 transgenic plants which exhibit improved growth underlow-nitrogen conditions.

U.S. Pat. No. 6,084,153 to Good et al. discloses the use of a stressresponsive promoter to control the expression of Alanine AmineTransferase (AlaAT) and transgenic canola plants with improved droughtand nitrogen deficiency tolerance when compared to control plants.

The ever-increasing world population and the decreasing availability inarable land for agriculture affect the yield of plants and plant-relatedproducts. The global shortage of water supply, desertification, abioticstress (ABS) conditions (e.g., salinity, drought, flood, suboptimaltemperature and toxic chemical pollution), and/or limited nitrogen andfertilizer sources cause substantial damage to agricultural plants suchas major alterations in the plant metabolism, cell death, and decreasesin plant growth and crop productivity.

Drought is a gradual phenomenon, which involves periods of abnormallydry weather that persists long enough to produce serious hydrologicimbalances such as crop damage, water supply shortage and increasedsusceptibility to various diseases.

Salinity, high salt levels, affects one in five hectares of irrigatedland. None of the top five food crops, i.e., wheat, corn, rice,potatoes, and soybean, can tolerate excessive salt. Detrimental effectsof salt on plants result from both water deficit, which leads to osmoticstress (similar to drought stress), and the effect of excess sodium ionson critical biochemical processes. As with freezing and drought, highsalt causes water deficit; and the presence of high salt makes itdifficult for plant roots to extract water from their environment. Thus,salination of soils that are used for agricultural production is asignificant and increasing problem in regions that rely heavily onagriculture, and is worsen by over-utilization, over-fertilization andwater shortage, typically caused by climatic change and the demands ofincreasing population.

Suboptimal temperatures affect plant growth and development through thewhole plant life cycle. Thus, low temperatures reduce germination rateand high temperatures result in leaf necrosis. In addition, matureplants that are exposed to excess of heat may experience heat shock,which may arise in various organs, including leaves and particularlyfruit, when transpiration is insufficient to overcome heat stress. Heatalso damages cellular structures, including organelles and cytoskeleton,and impairs membrane function. Heat shock may produce a decrease inoverall protein synthesis, accompanied by expression of heat shockproteins, e.g., chaperones, which are involved in refolding proteinsdenatured by heat. High-temperature damage to pollen almost alwaysoccurs in conjunction with drought stress, and rarely occurs underwell-watered conditions. Combined stress can alter plant metabolism innovel ways. Excessive chilling conditions, e.g., low, but abovefreezing, temperatures affect crops of tropical origins, such assoybean, rice, maize, and cotton. Typical chilling damage includeswilting, necrosis, chlorosis or leakage of ions from cell membranes.Excessive light conditions, which occur under clear atmosphericconditions subsequent to cold late summer/autumn nights, can lead tophotoinhibition of photosynthesis (disruption of photosynthesis). Inaddition, chilling may lead to yield losses and lower product qualitythrough the delayed ripening of maize.

Nutrient deficiencies cause adaptations of the root architecture,particularly notably for example is the root proliferation withinnutrient rich patches to increase nutrient uptake. Nutrient deficienciescause also the activation of plant metabolic pathways which maximize theabsorption, assimilation and distribution processes such as byactivating architectural changes. Engineering the expression of thetriggered genes may cause the plant to exhibit the architectural changesand enhanced metabolism also under other conditions.

In addition, it is widely known that the plants usually respond to waterdeficiency by creating a deeper root system that allows access tomoisture located in deeper soil layers. Triggering this effect willallow the plants to access nutrients and water located in deeper soilhorizons particularly those readily dissolved in water like nitrates.

Yield is affected by various factors, such as, the number and size ofthe plant organs, plant architecture (for example, the number ofbranches), grains set length, number of filled grains, vigor (e.g.seedling), growth rate, root development, utilization of water,nutrients (e.g., nitrogen) and fertilizers, and stress tolerance.

Crops such as, corn, rice, wheat, canola and soybean account for overhalf of total human caloric intake, whether through direct consumptionof the seeds themselves or through consumption of meat products raisedon processed seeds or forage. Seeds are also a source of sugars,proteins and oils and metabolites used in industrial processes. Theability to increase plant yield, whether through increase dry matteraccumulation rate, modifying cellulose or lignin composition, increasestalk strength, enlarge meristem size, change of plant branchingpattern, erectness of leaves, increase in fertilization efficiency,enhanced seed dry matter accumulation rate, modification of seeddevelopment, enhanced seed filling or by increasing the content of oil,starch or protein in the seeds would have many applications inagricultural and non-agricultural uses such as in the biotechnologicalproduction of pharmaceuticals, antibodies or vaccines.

Studies have shown that plant adaptations to adverse environmentalconditions are complex genetic traits with polygenic nature.Conventional means for crop and horticultural improvements utilizeselective breeding techniques to identify plants having desirablecharacteristics. However, selective breeding is tedious, time consumingand has an unpredictable outcome. Furthermore, limited germplasmresources for yield improvement and incompatibility in crosses betweendistantly related plant species represent significant problemsencountered in conventional breeding. Advances in genetic engineeringhave allowed mankind to modify the germplasm of plants by expression ofgenes-of-interest in plants. Such a technology has the capacity togenerate crops or plants with improved economic, agronomic orhorticultural traits.

WO publication No. 2009/013750 discloses genes, constructs and methodsof increasing abiotic stress tolerance, biomass and/or yield in plantsgenerated thereby.

WO publication No. 2008/122980 discloses genes constructs and methodsfor increasing oil content, growth rate and biomass of plants.

WO publication No. 2008/075364 discloses polynucleotides involved inplant fiber development and methods of using same.

WO publication No. 2007/049275 discloses isolated polypeptides,polynucleotides encoding same, transgenic plants expressing same andmethods of using same for increasing fertilizer use efficiency, plantabiotic stress tolerance and biomass.

WO publication No. 2004/104162 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2005/121364 discloses polynucleotides andpolypeptides involved in plant fiber development and methods of usingsame for improving fiber quality, yield and/or biomass of a fiberproducing plant.

WO publication No. 2007/020638 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2009/083958 discloses methods of increasing water useefficiency, fertilizer use efficiency, biotic/abiotic stress tolerance,yield and biomass in plant and plants generated thereby.

WO publication No. 2010/020941 discloses methods of increasing nitrogenuse efficiency, abiotic stress tolerance, yield and biomass in plantsand plants generated thereby.

WO publication No. 2009/141824 discloses isolated polynucleotides andmethods using same for increasing plant utility.

WO publication No. 2010/076756 discloses isolated polynucleotides forincreasing abiotic stress tolerance, yield, biomass, growth rate, vigor,oil content, fiber yield, fiber quality, and/or nitrogen use efficiencyof a plant.

WO publication No. 2004/081173 discloses novel plant derived regulatorysequences and constructs and methods of using such sequences fordirecting expression of exogenous polynucleotide sequences in plants.

WO publication No. 2010/049897 discloses isolated polynucleotides andpolypeptides and methods of using same for increasing plant yield,biomass, growth rate, vigor, oil content, abiotic stress tolerance ofplants and nitrogen use efficiency.

WO publication No. 2004/111183 discloses nucleotide sequences forregulating gene expression in plant trichomes and constructs and methodsutilizing same.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency, yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide at least 80% identical to SEQ ID NO: 482,470-481, 483-784, 2398-3817 or 3818, thereby increasing the nitrogen useefficiency, yield, biomass, growth rate, vigor, oil content, fiberyield, fiber quality, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency, yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide selected from the group consisting of SEQ ID NOs:482, 470-481, 483-784 and 2398-3818, thereby increasing the nitrogen useefficiency, yield, biomass, growth rate, vigor, oil content, fiberyield, fiber quality, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency, yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising a nucleic acid sequenceat least 80% identical to SEQ ID NO: 277, 1-276, 278-469, 785-2396 or2397, thereby increasing the nitrogen use efficiency, yield, biomass,growth rate, vigor, oil content, fiber yield, fiber quality, and/orabiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing nitrogen use efficiency, yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising the nucleic acidsequence selected from the group consisting of SEQ ID NOs: 277, 1-276,278-469 and 785-2397, thereby increasing the nitrogen use efficiency,yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises an amino acid sequenceat least 80% homologous to the amino acid sequence set forth in SEQ IDNO: 482, 470-481, 483-784, 2398-3817 or 3818, wherein the amino acidsequence is capable of increasing nitrogen use efficiency, yield,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises the amino acid sequenceselected from the group consisting of SEQ ID NOs: 482, 470-481, 483-784and 2398-3818.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence at least 80% identical to SEQ ID NO: 277, 1-276, 278-469,785-2396 or 2397, wherein the nucleic acid sequence is capable ofincreasing nitrogen use efficiency, yield, biomass, growth rate, vigor,oil content, fiber yield, fiber quality, and/or abiotic stress toleranceof a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising the nucleic acidsequence selected from the group consisting of SEQ ID NOs: 277, 1-276,278-469 and 785-2397.

According to an aspect of some embodiments of the present inventionthere is provided a nucleic acid construct comprising the isolatedpolynucleotide of some embodiments of the invention, and a promoter fordirecting transcription of the nucleic acid sequence in a host cell.

According to an aspect of some embodiments of the present inventionthere is provided a method of generating a transgenic plant comprisingtransforming within the plant the nucleic acid construct of someembodiments of the invention, thereby generating the transgenic plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of generating a transgenic plant comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide at least 80% identical toSEQ ID NO: 482, 470-481, 483-784, 2398-3817 or 3818, thereby generatingthe transgenic plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of generating a transgenic plant comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide selected from the groupconsisting of SEQ ID NOs: 482, 470-481, 483-784, and 2398-3818, therebygenerating the transgenic plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of generating a transgenic plant comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence at least 80% identical to SEQ ID NO: 277, 1-276,278-469, 785-2396 or 2397, thereby generating the transgenic plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of generating a transgenic plant comprisingexpressing within the plant an exogenous polynucleotide selected fromthe group consisting of SEQ ID NOs: 277, 1-276, 278-469 and 785-2397,thereby generating the transgenic plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising an amino acidsequence at least 80% homologous to SEQ ID NO: 482, 470-481, 483-784,2398-3817 or 3818, wherein the amino acid sequence is capable ofincreasing nitrogen use efficiency, yield, biomass, growth rate, vigor,oil content, fiber yield, fiber quality, and/or abiotic stress toleranceof a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising the amino acidsequence selected from the group consisting of SEQ ID NOs: 482, 470-481,483-784 and 2398-3818.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polynucleotideof some embodiments of the invention, or the nucleic acid construct ofsome embodiments of the invention.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polypeptide ofsome embodiments of the invention.

According to an aspect of some embodiments of the present inventionthere is provided a transgenic plant comprising the nucleic acidconstruct of some embodiments of the invention.

According to an aspect of some embodiments of the present inventionthere is provided a transgenic plant exogenously expressing thepolynucleotide of some embodiments of the invention, the nucleic acidconstruct of some embodiments of the invention and/or the polypeptide ofsome embodiments of the invention.

According to some embodiments of the invention, the nucleic acidsequence encodes an amino acid sequence selected from the groupconsisting of SEQ ID NOs: 482, 470-481, 483-784 and 2398-3818.

According to some embodiments of the invention, the nucleic acidsequence is selected from the group consisting of SEQ ID NOs: 277,1-276, 278-469 and 785-2397.

According to some embodiments of the invention, the polynucleotideconsists of the nucleic acid sequence selected from the group consistingof SEQ ID NOs: 277, 1-276, 278-469 and 785-2397.

According to some embodiments of the invention, the nucleic acidsequence encodes the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 482, 470-481, 483-784 and 2398-3818.

According to some embodiments of the invention, the plant cell formspart of a plant.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

According to some embodiments of the invention, the abiotic stress isselected from the group consisting of salinity, drought, waterdeprivation, flood, etiolation, low temperature, high temperature, heavymetal toxicity, anaerobiosis, nutrient deficiency, nutrient excess,atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the yield comprises seedyield or oil yield.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder nitrogen-limiting conditions.

According to some embodiments of the invention, the promoter isheterologous to the isolated polynucleotide and/or to the host cell.

Unless otherwise defined, all technical and/or scientific terms usedherein have the same meaning as commonly understood by one of ordinaryskill in the art to which the invention pertains. Although methods andmaterials similar or equivalent to those described herein can be used inthe practice or testing of embodiments of the invention, exemplarymethods and/or materials are described below. In case of conflict, thepatent specification, including definitions, will control. In addition,the materials, methods, and examples are illustrative only and are notintended to be necessarily limiting.

BRIEF DESCRIPTION OF THE DRAWINGS

Some embodiments of the invention are herein described, by way ofexample only, with reference to the accompanying drawings. With specificreference now to the drawings in detail, it is stressed that theparticulars shown are by way of example and for purposes of illustrativediscussion of embodiments of the invention. In this regard, thedescription taken with the drawings makes apparent to those skilled inthe art how embodiments of the invention may be practiced.

In the drawings:

FIG. 1 is a schematic illustration of the modified pGI binary plasmidcontaining the new At6669 promoter (SEQ ID NO:3829) and the GUSintron(pQYN_6669) used for expressing the isolated polynucleotide sequences ofsome embodiments of the invention. RB—T-DNA right border; LB—T-DNA leftborder; MCS—Multiple cloning site; RE—any restriction enzyme; NOSpro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene;NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylationsignal); GUSintron—the GUS reporter gene (coding sequence and intron).The isolated polynucleotide sequences of the invention were cloned intothe vector while replacing the GUSintron reporter gene.

FIG. 2 is a schematic illustration of the modified pGI binary plasmidcontaining the new At6669 promoter (SEQ ID NO:3829) (pQFN or pQFNc) usedfor expressing the isolated polynucleotide sequences of some embodimentsof the invention. RB—T-DNA right border; LB—T-DNA left border;MCS—Multiple cloning site; RE—any restriction enzyme; NOS pro=nopalinesynthase promoter; NPT-II=neomycin phosphotransferase gene; NOSter=nopaline synthase terminator; Poly-A signal (polyadenylationsignal); GUSintron—the GUS reporter gene (coding sequence and intron).The isolated polynucleotide sequences of the invention were cloned intothe MCS of the vector.

FIGS. 3A-3F are images depicting visualization of root development oftransgenic plants exogenously expressing the polynucleotide of someembodiments of the invention when grown in transparent agar plates undernormal (FIGS. 3A-3B), osmotic stress (15% PEG; FIGS. 3C-3D) ornitrogen-limiting (FIGS. 3E-3F) conditions. The different transgeneswere grown in transparent agar plates for 17 days (7 days nursery and 10days after transplanting). The plates were photographed every 3-4 daysstarting at day 1 after transplanting. FIG. 3A—An image of a photographof plants taken following 10 after transplanting days on agar plateswhen grown under normal (standard) conditions. FIG. 3B—An image of rootanalysis of the plants shown in FIG. 3A in which the lengths of theroots measured are represented by arrows. FIG. 3C—An image of aphotograph of plants taken following 10 days after transplanting on agarplates, grown under high osmotic (PEG 15%) conditions. FIG. 3D—An imageof root analysis of the plants shown in FIG. 3C in which the lengths ofthe roots measured are represented by arrows. FIG. 3E—An image of aphotograph of plants taken following 10 days after transplanting on agarplates, grown under low nitrogen conditions. FIG. 3F—An image of rootanalysis of the plants shown in FIG. 3E in which the lengths of theroots measured are represented by arrows.

FIG. 4 is a schematic illustration of the modified pGI binary plasmidcontaining the Root Promoter (pQNa_RP; SEQ ID NO:3830) used forexpressing the isolated polynucleotide sequences of some embodiments ofthe invention. RB—T-DNA right border; LB—T-DNA left border; NOSpro=nopaline synthase promoter; NPT-II=neomycin phosphotransferase gene;NOS ter=nopaline synthase terminator; Poly-A signal (polyadenylationsignal); the isolated polynucleotide sequences according to someembodiments of the invention were cloned into the MCS of the vector.

FIG. 5 is a schematic illustration of the pQYN plasmid (5714 bp).

FIG. 6 is a schematic illustration of the pQFN plasmid (5967 bp).

FIG. 7 is a schematic illustration of the pQFYN plasmid (8004 bp).

FIG. 8 is a schematic illustration of pQXNc plasmid, which is a modifiedpGI binary plasmid used for expressing the isolated polynucleotidesequences of some embodiments of the invention. RB—T-DNA right border;LB—T-DNA left border; NOS pro=nopaline synthase promoter;NPT-II=neomycin phosphotransferase gene; NOS ter=nopaline synthaseterminator; RE=any restriction enzyme; Poly-A signal (polyadenylationsignal); 35S—the 35S promoter (SEQ ID NO:3827). The isolatedpolynucleotide sequences of some embodiments of the invention werecloned into the MCS (Multiple cloning site) of the vector.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to novelpolynucleotides and polypeptides, nucleic acid constructs comprisingsame, host cells expressing same, transgenic plants exogenouslyexpressing same and, more particularly, but not exclusively, to methodsof using same for increasing nitrogen use efficiency, fertilizer useefficiency, yield, growth rate, vigor, biomass, oil content, fiberyield, fiber quality, fiber length, abiotic stress tolerance and/orwater use efficiency of a plant.

Before explaining at least one embodiment of the invention in detail, itis to be understood that the invention is not necessarily limited in itsapplication to the details set forth in the following description orexemplified by the Examples. The invention is capable of otherembodiments or of being practiced or carried out in various ways.

The present inventors have identified novel polypeptides andpolynucleotides which can be used to increase nitrogen use efficiency,fertilizer use efficiency, yield, growth rate, vigor, biomass, oilcontent, fiber yield, fiber quality, fiber length, abiotic stresstolerance and/or water use efficiency of a plant.

Thus, as shown in the Examples section which follows, the presentinventors have utilized bioinformatics tools to identify polynucleotideswhich enhance fertilizer use efficiency (e.g., nitrogen use efficiency),yield (e.g., seed yield, oil yield, oil content), growth rate, biomass,vigor and/or abiotic stress tolerance of a plant. Genes, which affectthe trait-of-interest, were identified based on expression profiles ofgenes of several arabidopsis, rice, barley, sorghum, maize and tomatoecotypes/accessions and tissues, homology with genes known to affect thetrait-of-interest and using digital expression profile in specifictissues and conditions (Tables 1, 6, 12, 18, 26, 33, 38-39, 48, 54, 61,66-67, Examples 1, and 3-12 of the Examples section which follows).Homologous polypeptides and polynucleotides having the same functionwere also identified (Table 2, Example 2 of the Examples section whichfollows). Transgenic plants over-expressing the identifiedpolynucleotides (Table 68, Example 13 of the Examples section whichfollows) were found to exhibit increased plant performance undernitrogen-deficient or limiting conditions (Tables 69-74; Example 16 ofthe Examples section which follows) or under standard conditions (Tables75-80; Example 16 of the Examples section which follows). In addition,greenhouse seed maturation (GH-SM) assays revealed that the identifiedgenes increase nitrogen use efficiency (NUE), yield and growth rate ofplants under low or normal nitrogen conditions as determined by theincrease in biomass (e.g., dry weight, flowering inflorescenceemergence, leaf blade area, leaf number, plot coverage, rosette area anddiameter); harvest index; growth rate of leaf number, plot coverage androsette diameter; and yield (e.g., seed yield, 1000 seed weight) (Tables81-90; Example 17 of the Examples section which follows). Furthergreenhouse assays performed until bolting stage revealed that theidentified genes increase nitrogen use efficiency at limited and optimalnitrogen concentration as determined by the increase in plant biomass(dry weight, fresh weight, leaf number, plot coverage, rosette area anddiameter); and relative growth rate of leaf number, plot coverage androsette diameter (Tables 91-96; Example 18 of the Examples section whichfollows). Altogether, these results suggest the use of the novelpolynucleotides and polypeptides of the invention for increasingnitrogen use efficiency, yield (e.g., seed yield), growth rate, biomass,vigor and/or abiotic stress tolerance of a plant.

Thus, according to an aspect of some embodiments of the invention, thereis provided method of increasing fertilizer (e.g., nitrogen) useefficiency, yield, biomass, growth rate, vigor, oil content, fiberyield, fiber quality, and/or abiotic stress tolerance of a plant,comprising expressing within the plant an exogenous polynucleotidecomprising a nucleic acid sequence encoding a polypeptide at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about94%, at least about 95%, at least about 96%, at least about 97%, atleast about 98%, at least about 99%, or more say 100% homologous to theamino acid sequence selected from the group consisting of SEQ ID NOs:470-784 and 2398-3818, thereby increasing the nitrogen use efficiency,yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, and/or abiotic stress tolerance of the plant.

As used herein the phrase “fertilizer use efficiency” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per fertilizer unit applied. Themetabolic process can be the uptake, spread, absorbent, accumulation,relocation (within the plant) and use of one or more of the minerals andorganic moieties absorbed by the plant, such as nitrogen, phosphatesand/or potassium.

As used herein the phrase “fertilizer-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) of afertilizer applied which is below the level needed for normal plantmetabolism, growth, reproduction and/or viability.

As used herein the phrase “nitrogen use efficiency (NUE)” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per nitrogen unit applied. The metabolicprocess can be the uptake, spread, absorbent, accumulation, relocation(within the plant) and use of nitrogen absorbed by the plant.

As used herein the phrase “nitrogen-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) ofnitrogen (e.g., ammonium or nitrate) applied which is below the levelneeded for normal plant metabolism, growth, reproduction and/orviability.

Improved plant NUE and FUE is translated in the field into eitherharvesting similar quantities of yield, while implementing lessfertilizers, or increased yields gained by implementing the same levelsof fertilizers. Thus, improved NUE or FUE has a direct effect on plantyield in the field. Thus, the polynucleotides and polypeptides of someembodiments of the invention positively affect plant yield, seed yield,and plant biomass. In addition, the benefit of improved plant NUE willcertainly improve crop quality and biochemical constituents of the seedsuch as protein yield and oil yield.

It should be noted that improved ABST will confer plants with improvedvigor also under non-stress conditions, resulting in crops havingimproved biomass and/or yield e.g., elongated fibers for the cottonindustry, higher oil content.

As used herein the phrase “plant yield” refers to the amount (e.g., asdetermined by weight or size) or quantity (numbers) of tissues or organsproduced per plant or per growing season. Hence increased yield couldaffect the economic benefit one can obtain from the plant in a certaingrowing area and/or growing time.

It should be noted that a plant yield can be affected by variousparameters including, but not limited to, plant biomass; plant vigor;growth rate; seed yield; seed or grain quantity; seed or grain quality;oil yield; content of oil, starch and/or protein in harvested organs(e.g., seeds or vegetative parts of the plant); number of flowers(florets) per panicle (expressed as a ratio of number of filled seedsover number of primary panicles); harvest index; number of plants grownper area; number and size of harvested organs per plant and per area;number of plants per growing area (density); number of harvested organsin field; total leaf area; carbon assimilation and carbon partitioning(the distribution/allocation of carbon within the plant); resistance toshade; number of harvestable organs (e.g. seeds), seeds per pod, weightper seed; and modified architecture [such as increase stalk diameter,thickness or improvement of physical properties (e.g. elasticity)].

As used herein the phrase “seed yield” refers to the number or weight ofthe seeds per plant, seeds per pod, or per growing area or to the weightof a single seed, or to the oil extracted per seed. Hence seed yield canbe affected by seed dimensions (e.g., length, width, perimeter, areaand/or volume), number of (filled) seeds and seed filling rate and byseed oil content. Hence increase seed yield per plant could affect theeconomic benefit one can obtain from the plant in a certain growing areaand/or growing time; and increase seed yield per growing area could beachieved by increasing seed yield per plant, and/or by increasing numberof plants grown on the same given area.

The term “seed” (also referred to as “grain” or “kernel”) as used hereinrefers to a small embryonic plant enclosed in a covering called the seedcoat (usually with some stored food), the product of the ripened ovuleof gymnosperm and angiosperm plants which occurs after fertilization andsome growth within the mother plant.

The phrase “oil content” as used herein refers to the amount of lipidsin a given plant organ, either the seeds (seed oil content) or thevegetative portion of the plant (vegetative oil content) and istypically expressed as percentage of dry weight (10% humidity of seeds)or wet weight (for vegetative portion).

It should be noted that oil content is affected by intrinsic oilproduction of a tissue (e.g., seed, vegetative portion), as well as themass or size of the oil-producing tissue per plant or per growth period.

In one embodiment, increase in oil content of the plant can be achievedby increasing the size/mass of a plant's tissue(s) which comprise oilper growth period. Thus, increased oil content of a plant can beachieved by increasing the yield, growth rate, biomass and vigor of theplant.

As used herein the phrase “plant biomass” refers to the amount (e.g.,measured in grams of air-dry tissue) of a tissue produced from the plantin a growing season, which could also determine or affect the plantyield or the yield per growing area. An increase in plant biomass can bein the whole plant or in parts thereof such as aboveground (harvestable)parts, vegetative biomass, roots and seeds.

As used herein the phrase “growth rate” refers to the increase in plantorgan/tissue size per time (can be measured in cm² per day).

As used herein the phrase “plant vigor” refers to the amount (measuredby weight) of tissue produced by the plant in a given time. Henceincreased vigor could determine or affect the plant yield or the yieldper growing time or growing area. In addition, early vigor (seed and/orseedling) results in improved field stand.

Improving early vigor is an important objective of modern rice breedingprograms in both temperate and tropical rice cultivars. Long roots areimportant for proper soil anchorage in water-seeded rice. Where rice issown directly into flooded fields, and where plants must emerge rapidlythrough water, longer shoots are associated with vigor. Wheredrill-seeding is practiced, longer mesocotyls and coleoptiles areimportant for good seedling emergence. The ability to engineer earlyvigor into plants would be of great importance in agriculture. Forexample, poor early vigor has been a limitation to the introduction ofmaize (Zea mays L.) hybrids based on Corn Belt germplasm in the EuropeanAtlantic.

It should be noted that a plant yield can be determined under stress(e.g., abiotic stress, nitrogen-limiting conditions) and/or non-stress(normal) conditions.

As used herein, the phrase “non-stress conditions” refers to the growthconditions (e.g., water, temperature, light-dark cycles, humidity, saltconcentration, fertilizer concentration in soil, nutrient supply such asnitrogen, phosphorous and/or potassium), that do not significantly gobeyond the everyday climatic and other abiotic conditions that plantsmay encounter, and which allow optimal growth, metabolism, reproductionand/or viability of a plant at any stage in its life cycle (e.g., in acrop plant from seed to a mature plant and back to seed again). Personsskilled in the art are aware of normal soil conditions and climaticconditions for a given plant in a given geographic location. It shouldbe noted that while the non-stress conditions may include some mildvariations from the optimal conditions (which vary from one type/speciesof a plant to another), such variations do not cause the plant to ceasegrowing without the capacity to resume growth.

The phrase “abiotic stress” as used herein refers to any adverse effecton metabolism, growth, reproduction and/or viability of a plant.Accordingly, abiotic stress can be induced by suboptimal environmentalgrowth conditions such as, for example, salinity, water deprivation,flooding, freezing, low or high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, atmospheric pollution or UVirradiation. The implications of abiotic stress are discussed in theBackground section.

The phrase “abiotic stress tolerance” as used herein refers to theability of a plant to endure an abiotic stress without suffering asubstantial alteration in metabolism, growth, productivity and/orviability.

Plants are subject to a range of environmental challenges. Several ofthese, including salt stress, general osmotic stress, drought stress andfreezing stress, have the ability to impact whole plant and cellularwater availability. Not surprisingly, then, plant responses to thiscollection of stresses are related. Zhu (2002) Ann. Rev. Plant Biol. 53:247-273 et al. note that “most studies on water stress signaling havefocused on salt stress primarily because plant responses to salt anddrought are closely related and the mechanisms overlap”. Many examplesof similar responses and pathways to this set of stresses have beendocumented. For example, the CBF transcription factors have been shownto condition resistance to salt, freezing and drought (Kasuga et al.(1999) Nature Biotech. 17: 287-291). The Arabidopsis rd29B gene isinduced in response to both salt and dehydration stress, a process thatis mediated largely through an ABA signal transduction process (Uno etal. (2000) Proc. Natl. Acad. Sci. USA 97: 11632-11637), resulting inaltered activity of transcription factors that bind to an upstreamelement within the rd29B promoter. In Mesembryanthemum crystallinum (iceplant), Patharker and Cushman have shown that a calcium-dependentprotein kinase (McCDPK1) is induced by exposure to both drought and saltstresses (Patharker and Cushman (2000) Plant J. 24: 679-691). Thestress-induced kinase was also shown to phosphorylate a transcriptionfactor, presumably altering its activity, although transcript levels ofthe target transcription factor are not altered in response to salt ordrought stress. Similarly, Saijo et al. demonstrated that a ricesalt/drought-induced calmodulin-dependent protein kinase (OsCDPK7)conferred increased salt and drought tolerance to rice whenoverexpressed (Saijo et al. (2000) Plant J. 23: 319-327).

Exposure to dehydration invokes similar survival strategies in plants asdoes freezing stress (see, for example, Yelenosky (1989) Plant Physiol89: 444-451) and drought stress induces freezing tolerance (see, forexample, Siminovitch et al. (1982) Plant Physiol 69: 250-255; and Guy etal. (1992) Planta 188: 265-270). In addition to the induction ofcold-acclimation proteins, strategies that allow plants to survive inlow water conditions may include, for example, reduced surface area, orsurface oil or wax production. In another example increased solutecontent of the plant prevents evaporation and water loss due to heat,drought, salinity, osmoticum, and the like therefore providing a betterplant tolerance to the above stresses.

It will be appreciated that some pathways involved in resistance to onestress (as described above), will also be involved in resistance toother stresses, regulated by the same or homologous genes. Of course,the overall resistance pathways are related, not identical, andtherefore not all genes controlling resistance to one stress willcontrol resistance to the other stresses. Nonetheless, if a geneconditions resistance to one of these stresses, it would be apparent toone skilled in the art to test for resistance to these related stresses.Methods of assessing stress resistance are further provided in theExamples section which follows.

As used herein the phrase “water use efficiency (WUE)” refers to thelevel of organic matter produced per unit of water consumed by theplant, i.e., the dry weight of a plant in relation to the plant's wateruse, e.g., the biomass produced per unit transpiration.

The term “fiber” is usually inclusive of thick-walled conducting cellssuch as vessels and tracheids and to fibrillar aggregates of manyindividual fiber cells. Hence, the term “fiber” refers to (a)thick-walled conducting and non-conducting cells of the xylem; (b)fibers of extraxylary origin, including those from phloem, bark, groundtissue, and epidermis; and (c) fibers from stems, leaves, roots, seeds,and flowers or inflorescences (such as those of Sorghum vulgare used inthe manufacture of brushes and brooms).

Example of fiber producing plants, include, but are not limited to,agricultural crops such as cotton, silk cotton tree (Kapok, Ceibapentandra), desert willow, creosote bush, winterfat, balsa, kenaf,roselle, jute, sisal abaca, flax, corn, sugar cane, hemp, ramie, kapok,coir, bamboo, spanish moss and Agave spp. (e.g. sisal).

As used herein the phrase “fiber quality” refers to at least one fiberparameter which is agriculturally desired, or required in the fiberindustry (further described hereinbelow). Examples of such parameters,include but are not limited to, fiber length, fiber strength, fiberfitness, fiber weight per unit length, maturity ratio and uniformity(further described hereinbelow.

Cotton fiber (lint) quality is typically measured according to fiberlength, strength and fineness. Accordingly, the lint quality isconsidered higher when the fiber is longer, stronger and finer.

As used herein the phrase “fiber yield” refers to the amount or quantityof fibers produced from the fiber producing plant.

As used herein the term “increasing” refers to at least about 2%, atleast about 3%, at least about 4%, at least about 5%, at least about10%, at least about 15%, at least about 20%, at least about 30%, atleast about 40%, at least about 50%, at least about 60%, at least about70%, at least about 80%, increase in nitrogen use efficiency, yield,seed yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, and/or abiotic stress tolerance of a plant as compared to anative plant [i.e., a plant not modified with the biomolecules(polynucleotide or polypeptides) of the invention, e.g., anon-transformed plant of the same species which is grown under the same(e.g., identical) growth conditions].

The phrase “expressing within the plant an exogenous polynucleotide” asused herein refers to upregulating the expression level of an exogenouspolynucleotide within the plant by introducing the exogenouspolynucleotide into a plant cell or plant and expressing by recombinantmeans, as further described herein below.

As used herein “expressing” refers to expression at the mRNA andoptionally polypeptide level.

As used herein, the phrase “exogenous polynucleotide” refers to aheterologous nucleic acid sequence which may not be naturally expressedwithin the plant or which overexpression in the plant is desired. Theexogenous polynucleotide may be introduced into the plant in a stable ortransient manner, so as to produce a ribonucleic acid (RNA) moleculeand/or a polypeptide molecule. It should be noted that the exogenouspolynucleotide may comprise a nucleic acid sequence which is identicalor partially homologous to an endogenous nucleic acid sequence of theplant.

The term “endogenous” as used herein refers to any polynucleotide orpolypeptide which is present and/or naturally expressed within a plantor a cell thereof.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention comprises a nucleic acid sequenceencoding a polypeptide having an amino acid sequence at least about 80%,at least about 81%, at least about 82%, at least about 83%, at leastabout 84%, at least about 85%, at least about 86%, at least about 87%,at least about 88%, at least about 89%, at least about 90%, at leastabout 91%, at least about 92%, at least about 93%, at least about 94%,at least about 95%, at least about 96%, at least about 97%, at leastabout 98%, at least about 99%, or more say 100% homologous to the aminoacid sequence selected from the group consisting of SEQ ID NOs: 470-784and 2398-3818.

Homology (e.g., percent homology, identity+similarity) can be determinedusing any homology comparison software, including for example, theBlastP™ (protein Basic Local Alignment Search Tool) or TBLASTN™(translated nucleotide databases using a protein query) software of theNational Center of Biotechnology Information (NCBI) such as by usingdefault parameters, when starting from a polypeptide sequence; or thetBLASTX™ (translated nucleotide databases using a translated nucleotidequery) algorithm (available via the NCBI) such as by using defaultparameters, which compares the six-frame conceptual translation productsof a nucleotide query sequence (both strands) against a protein sequencedatabase.

According to some embodiments of the invention, the term “homology” or“homologous” refers to identity of two or more nucleic acid sequences;or identity of two or more amino acid sequences.

Homologous sequences include both orthologous and paralogous sequences.The term “paralogous” relates to gene-duplications within the genome ofa species leading to paralogous genes. The term “orthologous” relates tohomologous genes in different organisms due to ancestral relationship.

One option to identify orthologues in monocot plant species is byperforming a reciprocal BLAST™ search. This may be done by a firstBLAST™ involving blasting the sequence-of-interest against any sequencedatabase, such as the publicly available NCBI database which may befound at: Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot)nlm (dot) nih (dot) gov. If orthologues in rice were sought, thesequence-of-interest would be blasted against, for example, the 28,469full-length cDNA clones from Oryza sativa Nipponbare available at NCBI.The BLAST™ results may be filtered. The full-length sequences of eitherthe filtered results or the non-filtered results are then blasted back(second BLAST™) against the sequences of the organism from which thesequence-of-interest is derived. The results of the first and secondBLAST™s are then compared. An orthologue is identified when the sequenceresulting in the highest score (best hit) in the first BLAST™ identifiesin the second BLAST™ the query sequence (the originalsequence-of-interest) as the best hit. Using the same rational aparalogue (homolog to a gene in the same organism) is found. In case oflarge sequence families, the ClustalW program may be used [HypertextTransfer Protocol://World Wide Web (dot) ebi (dot) ac (dot)uk/Tools/clustalw2/index (dot) html], followed by a neighbor-joiningtree (Hypertext Transfer Protocol://en (dot) wikipedia (dot)org/wiki/Neighbor-joining) which helps visualizing the clustering.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having an aminoacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, or moresay 100% identical to the amino acid sequence selected from the groupconsisting of SEQ ID NOs:470-784 and 2398-3818.

According to some embodiments of the invention, the method of increasingnitrogen use efficiency, yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, and/or abiotic stress tolerance ofa plant is effected by expressing within the plant an exogenouspolynucleotide comprising a nucleic acid sequence encoding a polypeptideat least at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, or more say100% identical to the amino acid sequence selected from the groupconsisting of SEQ ID NOs:470-784 and 2398-3818, thereby increasing thenitrogen use efficiency, yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, and/or abiotic stress tolerance ofthe plant.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO:470-784, 2398-3817 or 3818.

According to an aspect of some embodiments of the invention, the methodof increasing nitrogen use efficiency, yield, biomass, growth rate,vigor, oil content, fiber yield, fiber quality, and/or abiotic stresstolerance of a plant is effected by expressing within the plant anexogenous polynucleotide comprising a nucleic acid sequence encoding apolypeptide comprising an amino acid sequence selected from the groupconsisting of SEQ ID NOs:470-784 and 2398-3818, thereby increasing thenitrogen use efficiency, yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, and/or abiotic stress tolerance ofthe plant.

According to an aspect of some embodiments of the invention, there isprovided a method of increasing nitrogen use efficiency, yield, biomass,growth rate, vigor, oil content, fiber yield, fiber quality, and/orabiotic stress tolerance of a plant, comprising expressing within theplant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide selected from the group consisting of SEQ ID NOs:470-784 and 2398-3818, thereby increasing the nitrogen use efficiency,yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, and/or abiotic stress tolerance of the plant.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO: 470-784, 2398-3817 or 3818.

According to some embodiments of the invention the exogenouspolynucleotide comprises a nucleic acid sequence which is at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, e.g., 100%identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:1-469 and 785-2397.

According to an aspect of some embodiments of the invention, there isprovided a method of increasing nitrogen use efficiency, yield, biomass,growth rate, vigor, oil content, fiber yield, fiber quality, and/orabiotic stress tolerance of a plant, comprising expressing within theplant an exogenous polynucleotide comprising a nucleic acid sequence atleast about 80%, at least about 81%, at least about 82%, at least about83%, at least about 84%, at least about 85%, at least about 86%, atleast about 87%, at least about 88%, at least about 89%, at least about90%, at least about 91%, at least about 92%, at least about 93%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, e.g.,100% identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:1-469 and 785-2397, thereby increasing thenitrogen use efficiency, yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, and/or abiotic stress tolerance ofthe plant.

According to some embodiments of the invention, the homology is a globalhomology, i.e., an homology over the entire amino acid or nucleic acidsequences of the invention and not over portions thereof.

According to some embodiments of the invention, the identity is a globalidentity, i.e., an identity over the entire amino acid or nucleic acidsequences of the invention and not over portions thereof.

Identity (e.g., percent homology) can be determined using any homologycomparison software, including for example, the BlastN™ software of theNational Center of Biotechnology Information (NCBI) such as by usingdefault parameters.

According to some embodiments of the invention the exogenouspolynucleotide is at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:1-469 and 785-2397.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by SEQ ID NO:1-469, 785-2396 or 2397.

As used herein the term “polynucleotide” refers to a single or doublestranded nucleic acid sequence which is isolated and provided in theform of an RNA sequence, a complementary polynucleotide sequence (cDNA),a genomic polynucleotide sequence and/or a composite polynucleotidesequences (e.g., a combination of the above).

The term “isolated” refers to at least partially separated from thenatural environment e.g., from a plant cell.

As used herein the phrase “complementary polynucleotide sequence” refersto a sequence, which results from reverse transcription of messenger RNAusing a reverse transcriptase or any other RNA dependent DNA polymerase.Such a sequence can be subsequently amplified in vivo or in vitro usinga DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to asequence derived (isolated) from a chromosome and thus it represents acontiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers toa sequence, which is at least partially complementary and at leastpartially genomic. A composite sequence can include some exonalsequences required to encode the polypeptide of the present invention,as well as some intronic sequences interposing therebetween. Theintronic sequences can be of any source, including of other genes, andtypically will include conserved splicing signal sequences. Suchintronic sequences may further include cis acting expression regulatoryelements.

Nucleic acid sequences encoding the polypeptides of the presentinvention may be optimized for expression. Examples of such sequencemodifications include, but are not limited to, an altered G/C content tomore closely approach that typically found in the plant species ofinterest, and the removal of codons atypically found in the plantspecies commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriateDNA nucleotides for use within a structural gene or fragment thereofthat approaches codon usage within the plant of interest. Therefore, anoptimized gene or nucleic acid sequence refers to a gene in which thenucleotide sequence of a native or naturally occurring gene has beenmodified in order to utilize statistically-preferred orstatistically-favored codons within the plant. The nucleotide sequencetypically is examined at the DNA level and the coding region optimizedfor expression in the plant species determined using any suitableprocedure, for example as described in Sardana et al. (1996, Plant CellReports 15:677-681). In this method, the standard deviation of codonusage, a measure of codon usage bias, may be calculated by first findingthe squared proportional deviation of usage of each codon of the nativegene relative to that of highly expressed plant genes, followed by acalculation of the average squared deviation. The formula used is: 1SDCU=n=1 N [(Xn−Yn)/Yn]2/N, where Xn refers to the frequency of usage ofcodon n in highly expressed plant genes, where Yn to the frequency ofusage of codon n in the gene of interest and N refers to the totalnumber of codons in the gene of interest. A Table of codon usage fromhighly expressed genes of dicotyledonous plants is compiled using thedata of Murray et al. (1989, Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance withthe preferred codon usage for a particular plant cell type is based onthe direct use, without performing any extra statistical calculations,of codon optimization Tables such as those provided on-line at the CodonUsage Database through the NIAS (National Institute of AgrobiologicalSciences) DNA bank in Japan (Hypertext Transfer Protocol://World WideWeb (dot) kazusa (dot) or (dot) jp/codon/). The Codon Usage Databasecontains codon usage tables for a number of different species, with eachcodon usage Table having been statistically determined based on the datapresent in Genbank.

By using the above Tables to determine the most preferred or mostfavored codons for each amino acid in a particular species (for example,rice), a naturally-occurring nucleotide sequence encoding a protein ofinterest can be codon optimized for that particular plant species. Thisis effected by replacing codons that may have a low statisticalincidence in the particular species genome with corresponding codons, inregard to an amino acid, that are statistically more favored. However,one or more less-favored codons may be selected to delete existingrestriction sites, to create new ones at potentially useful junctions(5′ and 3′ ends to add signal peptide or termination cassettes, internalsites that might be used to cut and splice segments together to producea correct full-length sequence), or to eliminate nucleotide sequencesthat may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, inadvance of any modification, contain a number of codons that correspondto a statistically-favored codon in a particular plant species.Therefore, codon optimization of the native nucleotide sequence maycomprise determining which codons, within the native nucleotidesequence, are not statistically-favored with regards to a particularplant, and modifying these codons in accordance with a codon usage tableof the particular plant to produce a codon optimized derivative. Amodified nucleotide sequence may be fully or partially optimized forplant codon usage provided that the protein encoded by the modifiednucleotide sequence is produced at a level higher than the proteinencoded by the corresponding naturally occurring or native gene.Construction of synthetic genes by altering the codon usage is describedin for example PCT Patent Application 93/07278.

According to some embodiments of the invention, the exogenouspolynucleotide is a non-coding RNA.

As used herein the phrase ‘non-coding RNA” refers to an RNA moleculewhich does not encode an amino acid sequence (a polypeptide). Examplesof such non-coding RNA molecules include, but are not limited to, anantisense RNA, a pre-miRNA (precursor of a microRNA), or a precursor ofa Piwi-interacting RNA (piRNA).

Non-limiting examples of non-coding RNA polynucleotides are provided inSEQ ID NOs: 211-216, 264, 265, 466-469, 797, 927, 933, 939, 944 and 948.

Thus, the invention encompasses nucleic acid sequences describedhereinabove; fragments thereof, sequences hybridizable therewith,sequences homologous thereto, sequences encoding similar polypeptideswith different codon usage, altered sequences characterized bymutations, such as deletion, insertion or substitution of one or morenucleotides, either naturally occurring or man induced, either randomlyor in a targeted fashion.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:1-469 and 785-2397.

According to some embodiments of the invention the nucleic acid sequenceis capable of increasing nitrogen use efficiency, fertilizer useefficiency, yield, seed yield, growth rate, vigor, biomass, oil content,fiber yield, fiber quality, abiotic stress tolerance and/or water useefficiency of a plant.

According to some embodiments of the invention the isolatedpolynucleotide comprising the nucleic acid sequence selected from thegroup consisting of SEQ ID NOs:1-469 and 785-2397.

According to some embodiments of the invention the isolatedpolynucleotide is set forth by SEQ ID NO:1-469, 785-2396 or 2397.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to the amino acid sequence selectedfrom the group consisting of SEQ ID NOs: 470-784 and 2398-3818.

According to some embodiments of the invention the amino acid sequenceis capable of increasing nitrogen use efficiency, fertilizer useefficiency, yield, seed yield, growth rate, vigor, biomass, oil content,fiber yield, fiber quality, abiotic stress tolerance and/or water useefficiency of a plant.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises the amino acidsequence selected from the group consisting of SEQ ID NOs:470-784 and2398-3818.

According to an aspect of some embodiments of the invention, there isprovided a nucleic acid construct comprising the isolated polynucleotideof the invention, and a promoter for directing transcription of thenucleic acid sequence in a host cell.

The invention provides an isolated polypeptide comprising an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to an amino acid sequence selected fromthe group consisting of SEQ ID NOs: 470-784 and 2398-3818.

According to some embodiments of the invention, the polypeptidecomprising an amino acid sequence selected from the group consisting ofSEQ ID NOs:470-784 and 2398-3818.

According to some embodiments of the invention, the polypeptide is setforth by SEQ ID NO: 470-784, 2398-3817 or 3818.

The invention also encompasses fragments of the above describedpolypeptides and polypeptides having mutations, such as deletions,insertions or substitutions of one or more amino acids, either naturallyoccurring or man induced, either randomly or in a targeted fashion.

The term “‘plant” as used herein encompasses whole plants, ancestors andprogeny of the plants and plant parts, including seeds, shoots, stems,roots (including tubers), and plant cells, tissues and organs. The plantmay be in any form including suspension cultures, embryos, meristematicregions, callus tissue, leaves, gametophytes, sporophytes, pollen, andmicrospores. Plants that are particularly useful in the methods of theinvention include all plants which belong to the superfamilyViridiplantae, in particular monocotyledonous and dicotyledonous plantsincluding a fodder or forage legume, ornamental plant, food crop, tree,or shrub selected from the list comprising Acacia spp., Acer spp.,Actinidia spp., Aesculus spp., Agathis australis, Albizia amara,Alsophila tricolor, Andropogon spp., Arachis spp, Areca catechu, Asteliafragrans, Astragalus cicer, Baikiaea plurijuga, Betula spp., Brassicaspp., Bruguiera gymnorrhiza, Burkea africana, Butea frondosa, Cadabafarinosa, Calliandra spp, Camellia sinensis, Canna indica, Capsicumspp., Cassia spp., Centroema pubescens, Chacoomeles spp., Cinnamomumcassia, Coffea arabica, Colophospermum mopane, Coronillia varia,Cotoneaster serotina, Crataegus spp., Cucumis spp., Cupressus spp.,Cyathea dealbata, Cydonia oblonga, Cryptomeria japonica, Cymbopogonspp., Cynthea dealbata, Cydonia oblonga, Dalbergia monetaria, Davalliadivaricata, Desmodium spp., Dicksonia squarosa, Dibeteropogonamplectens, Dioclea spp, Dolichos spp., Dorycnium rectum, Echinochloapyramidalis, Ehraffia spp., Eleusine coracana, Eragrestis spp.,Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulalia vi/losa,Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingia spp,Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycinejavanica, Gliricidia spp, Gossypium hirsutum, Grevillea spp., Guibourtiacoleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus,Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffheliadissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia,Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex,Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihotesculenta, Medicago saliva, Metasequoia glyptostroboides, Musasapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryzaspp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petuniaspp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photiniaspp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara,Pogonarthria fleckii, Pogonaffhria squarrosa, Populus spp., Prosopiscineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis,Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhusnatalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia, Rosaspp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitysvefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghumbicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides,Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themedatriandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vacciniumspp., Vicia spp., Vitis vinifera, Watsonia pyramidata, Zantedeschiaaethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brusselssprouts, cabbage, canola, carrot, cauliflower, celery, collard greens,flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean,straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize,wheat, barley, rye, oat, peanut, pea, lentil and alfalfa, cotton,rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, atree, an ornamental plant, a perennial grass and a forage crop.Alternatively algae and other non-Viridiplantae can be used for themethods of the present invention.

According to some embodiments of the invention, the plant used by themethod of the invention is a crop plant such as rice, maize, wheat,barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean,sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea),flax, lupinus, rapeseed, tobacco, poplar and cotton.

According to some embodiments of the invention the plant is adicotyledonous plant.

According to some embodiments of the invention the plant is amonocotyledonous plant.

According to some embodiments of the invention, there is provided aplant cell exogenously expressing the polynucleotide of some embodimentsof the invention, the nucleic acid construct of some embodiments of theinvention and/or the polypeptide of some embodiments of the invention.

According to some embodiments of the invention, expressing the exogenouspolynucleotide of the invention within the plant is effected bytransforming one or more cells of the plant with the exogenouspolynucleotide, followed by generating a mature plant from thetransformed cells and cultivating the mature plant under conditionssuitable for expressing the exogenous polynucleotide within the matureplant.

According to some embodiments of the invention, the transformation iseffected by introducing to the plant cell a nucleic acid construct whichincludes the exogenous polynucleotide of some embodiments of theinvention and at least one promoter for directing transcription of theexogenous polynucleotide in a host cell (a plant cell). Further detailsof suitable transformation approaches are provided hereinbelow.

As mentioned, the nucleic acid construct according to some embodimentsof the invention comprises a promoter sequence and the isolatedpolynucleotide of the invention.

According to some embodiments of the invention, the isolatedpolynucleotide is operably linked to the promoter sequence.

A coding nucleic acid sequence is “operably linked” to a regulatorysequence (e.g., promoter) if the regulatory sequence is capable ofexerting a regulatory effect on the coding sequence linked thereto.

As used herein, the term “promoter” refers to a region of DNA which liesupstream of the transcriptional initiation site of a gene to which RNApolymerase binds to initiate transcription of RNA. The promoter controlswhere (e.g., which portion of a plant) and/or when (e.g., at which stageor condition in the lifetime of an organism) the gene is expressed.

According to some embodiments of the invention, the promoter isheterologous to the isolated polynucleotide and/or to the host cell.

Any suitable promoter sequence can be used by the nucleic acid constructof the present invention. Preferably the promoter is a constitutivepromoter, a tissue-specific, or an abiotic stress-inducible promoter.

According to some embodiments of the invention, the promoter is a plantpromoter, which is suitable for expression of the exogenouspolynucleotide in a plant cell.

Suitable constitutive promoters include, for example, CaMV 35S promoter[SEQ ID NO:3827 (pQFNC); SEQ ID NO:3833 (PJJ 35S from Brachypodium); SEQID NO:3834 (Odell et al., Nature 313:810-812, 1985)], Arabidopsis At6669promoter (SEQ ID NO:3826; see PCT Publication No. WO04081173A2 or thenew At6669 promoter (SEQ ID NO:3829); maize Ubi 1 (Christensen et al.,Plant Sol. Biol. 18:675-689, 1992); rice actin (McElroy et al., PlantCell 2:163-171, 1990); pEMU (Last et al., Theor. Appl. Genet.81:581-588, 1991); CaMV 19S (Nilsson et al., Physiol. Plant 100:456-462,1997); GOS2 (de Pater et al, Plant J Nov; 2(6):837-44, 1992); ubiquitin(Christensen et al, Plant Mol. Biol. 18: 675-689, 1992); Ubi 1 promoter(SEQ ID NO:3832); RBCS promoter (SEQ ID NO:3831); Rice cyclophilin(Bucholz et al, Plant Mol Biol. 25(5):837-43, 1994); Maize H3 histone(Lepetit et al, Mol. Gen. Genet. 231: 276-285, 1992); Actin 2 (An et al,Plant J. 10(1); 107-121, 1996) and Synthetic Super MAS (Ni et al., ThePlant Journal 7: 661-76, 1995). Other constitutive promoters includethose in U.S. Pat. Nos. 5,659,026, 5,608,149; 5,608,144; 5,604,121;5,569,597: 5,466,785; 5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to,leaf-specific promoters [such as described, for example, by Yamamoto etal., Plant J. 12:255-265, 1997; Kwon et al., Plant Physiol. 105:357-67,1994; Yamamoto et al., Plant Cell Physiol. 35:773-778, 1994; Gotor etal., Plant J. 3:509-18, 1993; Orozco et al., Plant Mol. Biol.23:1129-1138, 1993; and Matsuoka et al., Proc. Natl. Acad. Sci. USA90:9586-9590, 1993], seed-preferred promoters [e.g., Napin (originatedfrom Brassica napus which is characterized by a seed specific promoteractivity; Stuitje A. R. et. al. Plant Biotechnology Journal 1 (4):301-309; SEQ ID NO:3828), from seed specific genes (Simon, et al., PlantMol. Biol. 5. 191, 1985; Scofield, et al., J. Biol. Chem. 262: 12202,1987; Baszczynski, et al., Plant Mol. Biol. 14: 633, 1990), Brazil Nutalbumin (Pearson' et al., Plant Mol. Biol. 18: 235-245, 1992), legumin(Ellis, et al. Plant Mol. Biol. 10: 203-214, 1988), Glutelin (rice)(Takaiwa, et al., Mol. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al.,FEBS Letts. 221: 43-47, 1987), Zein (Matzke et al Plant Mol Biol, 143).323-32 1990), napA (Stalberg, et al, Planta 199: 515-519, 1996), WheatSPA (Albanietal, Plant Cell, 9: 171-184, 1997), sunflower oleosin(Cummins, et al., Plant Mol. Biol. 19: 873-876, 1992)], endospermspecific promoters [e.g., wheat LMW and HMW, glutenin-1 (Mol Gen Genet216:81-90, 1989; NAR 17:461-2), wheat a, b and g gliadins(EMBO3:1409-15, 1984), Barley ltrl promoter, barley B1, C, D hordein(Theor Appl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet250:750-60, 1996), Barley DOF (Mena et al, The Plant Journal, 116(1):53-62, 1998), Biz2 (EP99106056.7), Synthetic promoter (Vicente-Carbajosaet al., Plant J. 13: 629-640, 1998), rice prolamin NRP33, rice-globulinGlb-1 (Wu et al, Plant Cell Physiology 39(8) 885-889, 1998), ricealpha-globulin REB/OHP-1 (Nakase et al. Plant Mol. Biol. 33: 513-S22,1997), rice ADP-glucose PP (Trans Res 6:157-68, 1997), maize ESR genefamily (Plant J 12:235-46, 1997), sorghum gamma-kafirin (PMB 32:1029-35,1996)], embryo specific promoters [e.g., rice OSH1 (Sato et al, Proc.Natl. Acad. Sci. USA, 93: 8117-8122), KNOX (Postma-Haarsma et al, PlantMol. Biol. 39:257-71, 1999), rice oleosin (Wu et at, J. Biochem.,123:386, 1998)], and flower-specific promoters [e.g., AtPRP4, chalenesynthase (chsA) (Van der Meer, et al., Plant Mol. Biol. 15, 95-109,1990), LAT52 (Twell et al Mol. Gen Genet. 217:240-245; 1989),apetala-3], and root promoters such as the ROOTP promoter [SEQ ID NO:3830].

Suitable abiotic stress-inducible promoters include, but not limited to,salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al.,Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such asmaize rab17 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266,1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295,1997) and maize Ivr2 gene promoter (Pelleschi et. al., Plant Mol. Biol.39:373-380, 1999); heat-inducible promoters such as heat tomatohsp80-promoter from tomato (U.S. Pat. No. 5,187,267).

The nucleic acid construct of some embodiments of the invention canfurther include an appropriate selectable marker and/or an origin ofreplication. According to some embodiments of the invention, the nucleicacid construct utilized is a shuttle vector, which can propagate both inE. coli (wherein the construct comprises an appropriate selectablemarker and origin of replication) and be compatible with propagation incells. The construct according to the present invention can be, forexample, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus oran artificial chromosome.

The nucleic acid construct of some embodiments of the invention can beutilized to stably or transiently transform plant cells. In stabletransformation, the exogenous polynucleotide is integrated into theplant genome and as such it represents a stable and inherited trait. Intransient transformation, the exogenous polynucleotide is expressed bythe cell transformed but it is not integrated into the genome and assuch it represents a transient trait.

There are various methods of introducing foreign genes into bothmonocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev.Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al.,Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNAinto plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev.Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes, eds. Schell, J., and Vasil, L. K., Academic Publishers, SanDiego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds.Kung, S. and Arntzen, C. J., Butterworth Publishers, Boston, Mass.(1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and SomaticCell Genetics of Plants, Vol. 6, Molecular Biology of Plant NuclearGenes eds. Schell, J., and Vasil, L. K., Academic Publishers, San Diego,Calif. (1989) p. 52-68; including methods for direct uptake of DNA intoprotoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNAuptake induced by brief electric shock of plant cells: Zhang et al.Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986)319:791-793. DNA injection into plant cells or tissues by particlebombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al.Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990)79:206-209; by the use of micropipette systems: Neuhaus et al., Theor.Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant.(1990) 79:213-217; glass fibers or silicon carbide whiskertransformation of cell cultures, embryos or callus tissue, U.S. Pat. No.5,464,765 or by the direct incubation of DNA with germinating pollen,DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman,G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p.197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors thatcontain defined DNA segments that integrate into the plant genomic DNA.Methods of inoculation of the plant tissue vary depending upon the plantspecies and the Agrobacterium delivery system. A widely used approach isthe leaf disc procedure which can be performed with any tissue explantthat provides a good source for initiation of whole plantdifferentiation. See, e.g., Horsch et al. in Plant Molecular BiologyManual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. Asupplementary approach employs the Agrobacterium delivery system incombination with vacuum infiltration. The Agrobacterium system isespecially viable in the creation of transgenic dicotyledonous plants.

There are various methods of direct DNA transfer into plant cells. Inelectroporation, the protoplasts are briefly exposed to a strongelectric field. In microinjection, the DNA is mechanically injecteddirectly into the cells using very small micropipettes. In microparticlebombardment, the DNA is adsorbed on microprojectiles such as magnesiumsulfate crystals or tungsten particles, and the microprojectiles arephysically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The mostcommon method of plant propagation is by seed. Regeneration by seedpropagation, however, has the deficiency that due to heterozygositythere is a lack of uniformity in the crop, since seeds are produced byplants according to the genetic variances governed by Mendelian rules.Basically, each seed is genetically different and each will grow withits own specific traits. Therefore, it is preferred that the transformedplant be produced such that the regenerated plant has the identicaltraits and characteristics of the parent transgenic plant. Therefore, itis preferred that the transformed plant be regenerated bymicropropagation which provides a rapid, consistent reproduction of thetransformed plants.

Micropropagation is a process of growing new generation plants from asingle piece of tissue that has been excised from a selected parentplant or cultivar. This process permits the mass reproduction of plantshaving the preferred tissue expressing the fusion protein. The newgeneration plants which are produced are genetically identical to, andhave all of the characteristics of, the original plant. Micropropagationallows mass production of quality plant material in a short period oftime and offers a rapid multiplication of selected cultivars in thepreservation of the characteristics of the original transgenic ortransformed plant. The advantages of cloning plants are the speed ofplant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration ofculture medium or growth conditions between stages. Thus, themicropropagation process involves four basic stages: Stage one, initialtissue culturing; stage two, tissue culture multiplication; stage three,differentiation and plant formation; and stage four, greenhouseculturing and hardening. During stage one, initial tissue culturing, thetissue culture is established and certified contaminant-free. Duringstage two, the initial tissue culture is multiplied until a sufficientnumber of tissue samples are produced to meet production goals. Duringstage three, the tissue samples grown in stage two are divided and growninto individual plantlets. At stage four, the transformed plantlets aretransferred to a greenhouse for hardening where the plants' tolerance tolight is gradually increased so that it can be grown in the naturalenvironment.

According to some embodiments of the invention, the transgenic plantsare generated by transient transformation of leaf cells, meristematiccells or the whole plant.

Transient transformation can be effected by any of the direct DNAtransfer methods described above or by viral infection using modifiedplant viruses.

Viruses that have been shown to be useful for the transformation ofplant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus(BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation ofplants using plant viruses is described in U.S. Pat. No. 4,855,237 (beangolden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese PublishedApplication No. 63-14693 (TMV), EPA 194,809 (BV), EPA 278,667 (BV); andGluzman, Y. et al., Communications in Molecular Biology: Viral Vectors,Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirusparticles for use in expressing foreign DNA in many hosts, includingplants are described in WO 87/06261.

According to some embodiments of the invention, the virus used fortransient transformations is avirulent and thus is incapable of causingsevere symptoms such as reduced growth rate, mosaic, ring spots, leafroll, yellowing, streaking, pox formation, tumor formation and pitting.A suitable avirulent virus may be a naturally occurring avirulent virusor an artificially attenuated virus. Virus attenuation may be effectedby using methods well known in the art including, but not limited to,sub-lethal heating, chemical treatment or by directed mutagenesistechniques such as described, for example, by Kurihara and Watanabe(Molecular Plant Pathology 4:259-269, 2003), Gal-on et al. (1992),Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as,for example, the American Type culture Collection (ATCC) or by isolationfrom infected plants. Isolation of viruses from infected plant tissuescan be effected by techniques well known in the art such as described,for example by Foster and Tatlor, Eds. “Plant Virology Protocols: FromVirus Isolation to Transgenic Resistance (Methods in Molecular Biology(Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of aninfected plant believed to contain a high concentration of a suitablevirus, preferably young leaves and flower petals, are ground in a buffersolution (e.g., phosphate buffer solution) to produce a virus infectedsap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression ofnon-viral exogenous polynucleotide sequences in plants is demonstratedby the above references as well as by Dawson, W. O. et al., Virology(1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French etal. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters (1990)269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to thevirus itself. Alternatively, the virus can first be cloned into abacterial plasmid for ease of constructing the desired viral vector withthe foreign DNA. The virus can then be excised from the plasmid. If thevirus is a DNA virus, a bacterial origin of replication can be attachedto the viral DNA, which is then replicated by the bacteria.Transcription and translation of this DNA will produce the coat proteinwhich will encapsidate the viral DNA. If the virus is an RNA virus, thevirus is generally cloned as a cDNA and inserted into a plasmid. Theplasmid is then used to make all of the constructions. The RNA virus isthen produced by transcribing the viral sequence of the plasmid andtranslation of the viral genes to produce the coat protein(s) whichencapsidate the viral RNA.

In one embodiment, a plant viral polynucleotide is provided in which thenative coat protein coding sequence has been deleted from a viralpolynucleotide, a non-native plant viral coat protein coding sequenceand a non-native promoter, preferably the subgenomic promoter of thenon-native coat protein coding sequence, capable of expression in theplant host, packaging of the recombinant plant viral polynucleotide, andensuring a systemic infection of the host by the recombinant plant viralpolynucleotide, has been inserted. Alternatively, the coat protein genemay be inactivated by insertion of the non-native polynucleotidesequence within it, such that a protein is produced. The recombinantplant viral polynucleotide may contain one or more additional non-nativesubgenomic promoters. Each non-native subgenomic promoter is capable oftranscribing or expressing adjacent genes or polynucleotide sequences inthe plant host and incapable of recombination with each other and withnative subgenomic promoters. Non-native (foreign) polynucleotidesequences may be inserted adjacent the native plant viral subgenomicpromoter or the native and a non-native plant viral subgenomic promotersif more than one polynucleotide sequence is included. The non-nativepolynucleotide sequences are transcribed or expressed in the host plantunder control of the subgenomic promoter to produce the desiredproducts.

In a second embodiment, a recombinant plant viral polynucleotide isprovided as in the first embodiment except that the native coat proteincoding sequence is placed adjacent one of the non-native coat proteinsubgenomic promoters instead of a non-native coat protein codingsequence.

In a third embodiment, a recombinant plant viral polynucleotide isprovided in which the native coat protein gene is adjacent itssubgenomic promoter and one or more non-native subgenomic promoters havebeen inserted into the viral polynucleotide. The inserted non-nativesubgenomic promoters are capable of transcribing or expressing adjacentgenes in a plant host and are incapable of recombination with each otherand with native subgenomic promoters. Non-native polynucleotidesequences may be inserted adjacent the non-native subgenomic plant viralpromoters such that the sequences are transcribed or expressed in thehost plant under control of the subgenomic promoters to produce thedesired product.

In a fourth embodiment, a recombinant plant viral polynucleotide isprovided as in the third embodiment except that the native coat proteincoding sequence is replaced by a non-native coat protein codingsequence.

The viral vectors are encapsidated by the coat proteins encoded by therecombinant plant viral polynucleotide to produce a recombinant plantvirus. The recombinant plant viral polynucleotide or recombinant plantvirus is used to infect appropriate host plants. The recombinant plantviral polynucleotide is capable of replication in the host, systemicspread in the host, and transcription or expression of foreign gene(s)(exogenous polynucleotide) in the host to produce the desired protein.

Techniques for inoculation of viruses to plants may be found in Fosterand Taylor, eds. “Plant Virology Protocols: From Virus Isolation toTransgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol81)”, Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods inVirology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A.“Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A.“Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa,eds. “Principles and Techniques in Plant Virology”, VanNostrand-Reinhold, New York.

In addition to the above, the polynucleotide of the present inventioncan also be introduced into a chloroplast genome thereby enablingchloroplast expression.

A technique for introducing exogenous polynucleotide sequences to thegenome of the chloroplasts is known. This technique involves thefollowing procedures. First, plant cells are chemically treated so as toreduce the number of chloroplasts per cell to about one. Then, theexogenous polynucleotide is introduced via particle bombardment into thecells with the aim of introducing at least one exogenous polynucleotidemolecule into the chloroplasts. The exogenous polynucleotides selectedsuch that it is integratable into the chloroplast's genome viahomologous recombination which is readily effected by enzymes inherentto the chloroplast. To this end, the exogenous polynucleotide includes,in addition to a gene of interest, at least one polynucleotide stretchwhich is derived from the chloroplast's genome. In addition, theexogenous polynucleotide includes a selectable marker, which serves bysequential selection procedures to ascertain that all or substantiallyall of the copies of the chloroplast genomes following such selectionwill include the exogenous polynucleotide. Further details relating tothis technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507which are incorporated herein by reference. A polypeptide can thus beproduced by the protein expression system of the chloroplast and becomeintegrated into the chloroplast's inner membrane.

Since processes which increase nitrogen use efficiency, yield, biomass,growth rate, vigor, oil content, fiber yield, fiber quality, and/orabiotic stress tolerance of a plant can involve multiple genes actingadditively or in synergy (see, for example, in Quesda et al., PlantPhysiol. 130:951-063, 2002), the present invention also envisagesexpressing a plurality of exogenous polynucleotides in a single hostplant to thereby achieve superior effect on nitrogen use efficiency,yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, and/or abiotic stress tolerance of the plant.

Expressing a plurality of exogenous polynucleotides in a single hostplant can be effected by co-introducing multiple nucleic acidconstructs, each including a different exogenous polynucleotide, into asingle plant cell. The transformed cell can then be regenerated into amature plant using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by co-introducing into a singleplant-cell a single nucleic-acid construct including a plurality ofdifferent exogenous polynucleotides. Such a construct can be designedwith a single promoter sequence which can transcribe a polycistronicmessenger RNA including all the different exogenous polynucleotidesequences. To enable co-translation of the different polypeptidesencoded by the polycistronic messenger RNA, the polynucleotide sequencescan be inter-linked via an internal ribosome entry site (IRES) sequencewhich facilitates translation of polynucleotide sequences positioneddownstream of the IRES sequence. In this case, a transcribedpolycistronic RNA molecule encoding the different polypeptides describedabove will be translated from both the capped 5′ end and the twointernal IRES sequences of the polycistronic RNA molecule to therebyproduce in the cell all different polypeptides. Alternatively, theconstruct can include several promoter sequences each linked to adifferent exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality ofdifferent exogenous polynucleotides, can be regenerated into a matureplant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by introducing different nucleic acidconstructs, including different exogenous polynucleotides, into aplurality of plants. The regenerated transformed plants can then becross-bred and resultant progeny selected for superior abiotic stresstolerance, water use efficiency, fertilizer use efficiency, growth,biomass, yield and/or vigor traits, using conventional plant breedingtechniques.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

Non-limiting examples of abiotic stress conditions include, salinity,drought, water deprivation, excess of water (e.g., flood, waterlogging),etiolation, low temperature, high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, nutrient excess, atmosphericpollution and UV irradiation.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder fertilizer limiting conditions (e.g., nitrogen-limitingconditions). Non-limiting examples include growing the plant on soilswith low nitrogen content (40-50% Nitrogen of the content present undernormal or optimal conditions), or even under sever nitrogen deficiency(0-10% Nitrogen of the content present under normal or optimalconditions).

Thus, the invention encompasses plants exogenously expressing thepolynucleotide(s), the nucleic acid constructs and/or polypeptide(s) ofthe invention.

Once expressed within the plant cell or the entire plant, the level ofthe polypeptide encoded by the exogenous polynucleotide can bedetermined by methods well known in the art such as, activity assays,Western blots using antibodies capable of specifically binding thepolypeptide, Enzyme-Linked Immuno Sorbent Assay (ELISA),radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry,immunofluorescence and the like.

Methods of determining the level in the plant of the RNA transcribedfrom the exogenous polynucleotide are well known in the art and include,for example, Northern blot analysis, reverse transcription polymerasechain reaction (RT-PCR) analysis (including quantitative,semi-quantitative or real-time RT-PCR) and RNA-in situ hybridization.

The sequence information and annotations uncovered by the presentteachings can be harnessed in favor of classical breeding. Thus,sub-sequence data of those polynucleotides described above, can be usedas markers for marker assisted selection (MAS), in which a marker isused for indirect selection of a genetic determinant or determinants ofa trait of interest (e.g., biomass, growth rate, oil content, yield,abiotic stress tolerance, water use efficiency, nitrogen use efficiencyand/or fertilizer use efficiency). Nucleic acid data of the presentteachings (DNA or RNA sequence) may contain or be linked to polymorphicsites or genetic markers on the genome such as restriction fragmentlength polymorphism (RFLP), microsatellites and single nucleotidepolymorphism (SNP), DNA fingerprinting (DFP), amplified fragment lengthpolymorphism (AFLP), expression level polymorphism, polymorphism of theencoded polypeptide and any other polymorphism at the DNA or RNAsequence.

Examples of marker assisted selections include, but are not limited to,selection for a morphological trait (e.g., a gene that affects form,coloration, male sterility or resistance such as the presence or absenceof awn, leaf sheath coloration, height, grain color, aroma of rice);selection for a biochemical trait (e.g., a gene that encodes a proteinthat can be extracted and observed; for example, isozymes and storageproteins); selection for a biological trait (e.g., pathogen races orinsect biotypes based on host pathogen or host parasite interaction canbe used as a marker since the genetic constitution of an organism canaffect its susceptibility to pathogens or parasites).

The polynucleotides and polypeptides described hereinabove can be usedin a wide range of economical plants, in a safe and cost effectivemanner.

Plant lines exogenously expressing the polynucleotide or the polypeptideof the invention are screened to identify those that show the greatestincrease of the desired plant trait.

The effect of the transgene (the exogenous polynucleotide encoding thepolypeptide) on abiotic stress tolerance can be determined using knownmethods such as detailed below and in the Examples section whichfollows.

Abiotic stress tolerance—Transformed (i.e., expressing the transgene)and non-transformed (wild type) plants are exposed to an abiotic stresscondition, such as water deprivation, suboptimal temperature (lowtemperature, high temperature), nutrient deficiency, nutrient excess, asalt stress condition, osmotic stress, heavy metal toxicity,anaerobiosis, atmospheric pollution and UV irradiation.

Salinity tolerance assay—Transgenic plants with tolerance to high saltconcentrations are expected to exhibit better germination, seedlingvigor or growth in high salt. Salt stress can be effected in many wayssuch as, for example, by irrigating the plants with a hyperosmoticsolution, by cultivating the plants hydroponically in a hyperosmoticgrowth solution (e.g., Hoagland solution), or by culturing the plants ina hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MSmedium)]. Since different plants vary considerably in their tolerance tosalinity, the salt concentration in the irrigation water, growthsolution, or growth medium can be adjusted according to the specificcharacteristics of the specific plant cultivar or variety, so as toinflict a mild or moderate effect on the physiology and/or morphology ofthe plants (for guidelines as to appropriate concentration see,Bernstein and Kafkafi, Root Growth Under Salinity Stress In: PlantRoots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U.(editors) Marcel Dekker Inc., New York, 2002, and reference therein).

For example, a salinity tolerance test can be performed by irrigatingplants at different developmental stages with increasing concentrationsof sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl)applied from the bottom and from above to ensure even dispersal of salt.Following exposure to the stress condition the plants are frequentlymonitored until substantial physiological and/or morphological effectsappear in wild type plants. Thus, the external phenotypic appearance,degree of wilting and overall success to reach maturity and yieldprogeny are compared between control and transgenic plants.

Quantitative parameters of tolerance measured include, but are notlimited to, the average wet and dry weight, growth rate, leaf size, leafcoverage (overall leaf area), the weight of the seeds yielded, theaverage seed size and the number of seeds produced per plant.Transformed plants not exhibiting substantial physiological and/ormorphological effects, or exhibiting higher biomass than wild-typeplants, are identified as abiotic stress tolerant plants.

Osmotic tolerance test—Osmotic stress assays (including sodium chlorideand mannitol assays) are conducted to determine if an osmotic stressphenotype was sodium chloride-specific or if it was a general osmoticstress related phenotype. Plants which are tolerant to osmotic stressmay have more tolerance to drought and/or freezing. For salt and osmoticstress germination experiments, the medium is supplemented for examplewith 50 mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol.

Drought tolerance assay/Osmoticum assay—Tolerance to drought isperformed to identify the genes conferring better plant survival afteracute water deprivation. To analyze whether the transgenic plants aremore tolerant to drought, an osmotic stress produced by the non-ionicosmolyte sorbitol in the medium can be performed. Control and transgenicplants are germinated and grown in plant-agar plates for 4 days, afterwhich they are transferred to plates containing 500 mM sorbitol. Thetreatment causes growth retardation, then both control and transgenicplants are compared, by measuring plant weight (wet and dry), yield, andby growth rates measured as time to flowering.

Conversely, soil-based drought screens are performed with plantsoverexpressing the polynucleotides detailed above. Seeds from controlArabidopsis plants, or other transgenic plants overexpressing thepolypeptide of the invention are germinated and transferred to pots.Drought stress is obtained after irrigation is ceased accompanied byplacing the pots on absorbent paper to enhance the soil-drying rate.Transgenic and control plants are compared to each other when themajority of the control plants develop severe wilting. Plants arere-watered after obtaining a significant fraction of the control plantsdisplaying a severe wilting. Plants are ranked comparing to controls foreach of two criteria: tolerance to the drought conditions and recovery(survival) following re-watering.

Cold stress tolerance—To analyze cold stress, mature (25 day old) plantsare transferred to 4° C. chambers for 1 or 2 weeks, with constitutivelight. Later on plants are moved back to greenhouse. Two weeks laterdamages from chilling period, resulting in growth retardation and otherphenotypes, are compared between both control and transgenic plants, bymeasuring plant weight (wet and dry), and by comparing growth ratesmeasured as time to flowering, plant size, yield, and the like.

Heat stress tolerance—Heat stress tolerance is achieved by exposing theplants to temperatures above 34° C. for a certain period. Planttolerance is examined after transferring the plants back to 22° C. forrecovery and evaluation after 5 days relative to internal controls(non-transgenic plants) or plants not exposed to neither cold or heatstress.

Water use efficiency—can be determined as the biomass produced per unittranspiration. To analyze WUE, leaf relative water content can bemeasured in control and transgenic plants. Fresh weight (FW) isimmediately recorded; then leaves are soaked for 8 hours in distilledwater at room temperature in the dark, and the turgid weight (TW) isrecorded. Total dry weight (DW) is recorded after drying the leaves at60° C. to a constant weight. Relative water content (RWC) is calculatedaccording to the following Formula I:

RWC=[(FW−DW)/(TW−DW)]×100  Formula I

Fertilizer use efficiency—To analyze whether the transgenic plants aremore responsive to fertilizers, plants are grown in agar plates or potswith a limited amount of fertilizer, as described, for example, inExamples 16-18, hereinbelow and in Yanagisawa et al (Proc Natl Acad SciUSA. 2004; 101:7833-8). The plants are analyzed for their overall size,time to flowering, yield, protein content of shoot and/or grain. Theparameters checked are the overall size of the mature plant, its wet anddry weight, the weight of the seeds yielded, the average seed size andthe number of seeds produced per plant. Other parameters that may betested are: the chlorophyll content of leaves (as nitrogen plant statusand the degree of leaf verdure is highly correlated), amino acid and thetotal protein content of the seeds or other plant parts such as leavesor shoots, oil content, etc. Similarly, instead of providing nitrogen atlimiting amounts, phosphate or potassium can be added at increasingconcentrations. Again, the same parameters measured are the same aslisted above. In this way, nitrogen use efficiency (NUE), phosphate useefficiency (PUE) and potassium use efficiency (KUE) are assessed,checking the ability of the transgenic plants to thrive under nutrientrestraining conditions.

Nitrogen use efficiency—To analyze whether the transgenic plants (e.g.,Arabidopsis plants) are more responsive to nitrogen, plant are grown in0.75-3 mM (nitrogen deficient conditions) or 6-10 mM (optimal nitrogenconcentration). Plants are allowed to grow for additional 25 days oruntil seed production. The plants are then analyzed for their overallsize, time to flowering, yield, protein content of shoot and/orgrain/seed production. The parameters checked can be the overall size ofthe plant, wet and dry weight, the weight of the seeds yielded, theaverage seed size and the number of seeds produced per plant. Otherparameters that may be tested are: the chlorophyll content of leaves (asnitrogen plant status and the degree of leaf greenness is highlycorrelated), amino acid and the total protein content of the seeds orother plant parts such as leaves or shoots and oil content. Transformedplants not exhibiting substantial physiological and/or morphologicaleffects, or exhibiting higher measured parameters levels than wild-typeplants, are identified as nitrogen use efficient plants.

Nitrogen Use efficiency assay using plantlets—The assay is doneaccording to Yanagisawa-S. et al. with minor modifications (“Metabolicengineering with Dof1 transcription factor in plants: Improved nitrogenassimilation and growth under low-nitrogen conditions” Proc. Natl. Acad.Sci. USA 101, 7833-7838). Briefly, transgenic plants which are grown for7-10 days in 0.5×MS [Murashige-Skoog] supplemented with a selectionagent are transferred to two nitrogen-limiting conditions: MS media inwhich the combined nitrogen concentration (NH₄NO₃ and KNO₃) was 0.75 mM(nitrogen deficient conditions) or 6-15 mM (optimal nitrogenconcentration). Plants are allowed to grow for additional 30-40 days andthen photographed, individually removed from the Agar (the shoot withoutthe roots) and immediately weighed (fresh weight) for later statisticalanalysis. Constructs for which only T1 seeds are available are sown onselective media and at least 20 seedlings (each one representing anindependent transformation event) are carefully transferred to thenitrogen-limiting media. For constructs for which T2 seeds areavailable, different transformation events are analyzed. Usually, 20randomly selected plants from each event are transferred to thenitrogen-limiting media allowed to grow for 3-4 additional weeks andindividually weighed at the end of that period. Transgenic plants arecompared to control plants grown in parallel under the same conditions.Mock-transgenic plants expressing the uidA reporter gene (GUS) under thesame promoter or transgenic plants carrying the same promoter butlacking a reporter gene are used as control.

Nitrogen Determination—The procedure for N (nitrogen) concentrationdetermination in the structural parts of the plants involves thepotassium persulfate digestion method to convert organic N to NO₃ ⁻(Purcell and King 1996 Argon. J. 88:111-113, the modified Cd⁻ mediatedreduction of NO₃ ⁻ to NO₂ ⁻ (Vodovotz 1996 Biotechniques 20:390-394) andthe measurement of nitrite by the Griess assay (Vodovotz 1996, supra).The absorbance values are measured at 550 nm against a standard curve ofNaNO₂. The procedure is described in details in Samonte et al. 2006Agron. J. 98:168-176.

Germination tests—Germination tests compare the percentage of seeds fromtransgenic plants that could complete the germination process to thepercentage of seeds from control plants that are treated in the samemanner. Normal conditions are considered for example, incubations at 22°C. under 22-hour light 2-hour dark daily cycles. Evaluation ofgermination and seedling vigor is conducted between 4 and 14 days afterplanting. The basal media is 50% MS medium (Murashige and Skoog, 1962Plant Physiology 15, 473-497).

Germination is checked also at unfavorable conditions such as cold(incubating at temperatures lower than 10° C. instead of 22° C.) orusing seed inhibition solutions that contain high concentrations of anosmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM,300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrationsof salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).

The effect of the transgene on plant's vigor, growth rate, biomass,yield and/or oil content can be determined using known methods.

Plant vigor—The plant vigor can be calculated by the increase in growthparameters such as leaf area, fiber length, rosette diameter, plantfresh weight and the like per time.

Growth rate—The growth rate can be measured using digital analysis ofgrowing plants. For example, images of plants growing in greenhouse onplot basis can be captured every 3 days and the rosette area can becalculated by digital analysis. Rosette area growth is calculated usingthe difference of rosette area between days of sampling divided by thedifference in days between samples.

Evaluation of growth rate can be done by measuring plant biomassproduced, rosette area, leaf size or root length per time (can bemeasured in cm² per day of leaf area).

Relative growth area can be calculated using Formula II.

Relative growth rate area=Regression coefficient of area along timecourse  Formula II:

Thus, the relative growth area rate is in units of 1/day and lengthgrowth rate is in units of 1/day.

Seed yield—Evaluation of the seed yield per plant can be done bymeasuring the amount (weight or size) or quantity (i.e., number) of dryseeds produced and harvested from 8-16 plants and divided by the numberof plants.

For example, the total seeds from 8-16 plants can be collected, weightedusing e.g., an analytical balance and the total weight can be divided bythe number of plants. Seed yield per growing area can be calculated inthe same manner while taking into account the growing area given to asingle plant. Increase seed yield per growing area could be achieved byincreasing seed yield per plant, and/or by increasing number of plantscapable of growing in a given area.

In addition, seed yield can be determined via the weight of 1000 seeds.The weight of 1000 seeds can be determined as follows: seeds arescattered on a glass tray and a picture is taken. Each sample isweighted and then using the digital analysis, the number of seeds ineach sample is calculated.

The 1000 seeds weight can be calculated using formula II:

1000 Seed Weight=number of seed in sample/sample weight×1000  FormulaIII:

The Harvest Index can be calculated using Formula IV

Harvest Index=Average seed yield per plant/Average dry weight  FormulaIV:

Grain protein concentration—Grain protein content (g grain protein m⁻²)is estimated as the product of the mass of grain N (g grain N m⁻²)multiplied by the N/protein conversion ratio of k-5.13 (Mosse 1990,supra). The grain protein concentration is estimated as the ratio ofgrain protein content per unit mass of the grain (g grain protein kg⁻¹grain).

Fiber length—Fiber length can be measured using fibrograph. Thefibrograph system was used to compute length in terms of “Upper HalfMean” length. The upper half mean (UHM) is the average length of longerhalf of the fiber distribution. The fibrograph measures length in spanlengths at a given percentage point (Hypertext Transfer Protocol://WorldWide Web (dot) cottoninc (dot) com/ClassificationofCotton/?Pg=4#Length).

According to some embodiments of the invention, increased yield of cornmay be manifested as one or more of the following: increase in thenumber of plants per growing area, increase in the number of ears perplant, increase in the number of rows per ear, number of kernels per earrow, kernel weight, thousand kernel weight (1000-weight), earlength/diameter, increase oil content per kernel and increase starchcontent per kernel.

As mentioned, the increase of plant yield can be determined by variousparameters. For example, increased yield of rice may be manifested by anincrease in one or more of the following: number of plants per growingarea, number of panicles per plant, number of spikelets per panicle,number of flowers per panicle, increase in the seed filling rate,increase in thousand kernel weight (1000-weight), increase oil contentper seed, increase starch content per seed, among others. An increase inyield may also result in modified architecture, or may occur because ofmodified architecture.

Similarly, increased yield of soybean may be manifested by an increasein one or more of the following: number of plants per growing area,number of pods per plant, number of seeds per pod, increase in the seedfilling rate, increase in thousand seed weight (1000-weight), reduce podshattering, increase oil content per seed, increase protein content perseed, among others. An increase in yield may also result in modifiedarchitecture, or may occur because of modified architecture.

Increased yield of canola may be manifested by an increase in one ormore of the following: number of plants per growing area, number of podsper plant, number of seeds per pod, increase in the seed filling rate,increase in thousand seed weight (1000−weight), reduce pod shattering,increase oil content per seed, among others. An increase in yield mayalso result in modified architecture, or may occur because of modifiedarchitecture.

Increased yield of cotton may be manifested by an increase in one ormore of the following: number of plants per growing area, number ofbolls per plant, number of seeds per boll, increase in the seed fillingrate, increase in thousand seed weight (1000−weight), increase oilcontent per seed, improve fiber length, fiber strength, among others. Anincrease in yield may also result in modified architecture, or may occurbecause of modified architecture.

Oil content—The oil content of a plant can be determined by extractionof the oil from the seed or the vegetative portion of the plant.Briefly, lipids (oil) can be removed from the plant (e.g., seed) bygrinding the plant tissue in the presence of specific solvents (e.g.,hexane or petroleum ether) and extracting the oil in a continuousextractor. Indirect oil content analysis can be carried out usingvarious known methods such as Nuclear Magnetic Resonance (NMR)Spectroscopy, which measures the resonance energy absorbed by hydrogenatoms in the liquid state of the sample [See for example, Conway T F.and Earle F R., 1963, Journal of the American Oil Chemists' Society;Springer Berlin/Heidelberg, ISSN: 0003-021X (Print) 1558-9331 (Online)];the Near Infrared (NI) Spectroscopy, which utilizes the absorption ofnear infrared energy (1100-2500 nm) by the sample; and a methoddescribed in WO/2001/023884, which is based on extracting oil a solvent,evaporating the solvent in a gas stream which forms oil particles, anddirecting a light into the gas stream and oil particles which forms adetectable reflected light.

Thus, the present invention is of high agricultural value for promotingthe yield of commercially desired crops (e.g., biomass of vegetativeorgan such as poplar wood, or reproductive organ such as number of seedsor seed biomass).

Any of the transgenic plants described hereinabove or parts thereof maybe processed to produce a feed, meal, protein or oil preparation, suchas for ruminant animals.

The transgenic plants described hereinabove, which exhibit an increasedoil content can be used to produce plant oil (by extracting the oil fromthe plant).

The plant oil (including the seed oil and/or the vegetative portion oil)produced according to the method of the invention may be combined with avariety of other ingredients. The specific ingredients included in aproduct are determined according to the intended use. Exemplary productsinclude animal feed, raw material for chemical modification,biodegradable plastic, blended food product, edible oil, biofuel,cooking oil, lubricant, biodiesel, snack food, cosmetics, andfermentation process raw material. Exemplary products to be incorporatedto the plant oil include animal feeds, human food products such asextruded snack foods, breads, as a food binding agent, aquaculturefeeds, fermentable mixtures, food supplements, sport drinks, nutritionalfood bars, multi-vitamin supplements, diet drinks, and cereal foods.

According to some embodiments of the invention, the oil comprises a seedoil.

According to some embodiments of the invention, the oil comprises avegetative portion oil.

According to some embodiments of the invention, the plant cell forms apart of a plant.

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having”and their conjugates mean “including but not limited to”.

The term “consisting of” means “including and limited to”.

The term “consisting essentially of” means that the composition, methodor structure may include additional ingredients, steps and/or parts, butonly if the additional ingredients, steps and/or parts do not materiallyalter the basic and novel characteristics of the claimed composition,method or structure.

As used herein, the singular form “a”, “an” and “the” include pluralreferences unless the context clearly dictates otherwise. For example,the term “a compound” or “at least one compound” may include a pluralityof compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention maybe presented in a range format. It should be understood that thedescription in range format is merely for convenience and brevity andshould not be construed as an inflexible limitation on the scope of theinvention. Accordingly, the description of a range should be consideredto have specifically disclosed all the possible subranges as well asindividual numerical values within that range. For example, descriptionof a range such as from 1 to 6 should be considered to have specificallydisclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numberswithin that range, for example, 1, 2, 3, 4, 5, and 6. This appliesregardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to includeany cited numeral (fractional or integral) within the indicated range.The phrases “ranging/ranges between” a first indicate number and asecond indicate number and “ranging/ranges from” a first indicate number“to” a second indicate number are used herein interchangeably and aremeant to include the first and second indicated numbers and all thefractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniquesand procedures for accomplishing a given task including, but not limitedto, those manners, means, techniques and procedures either known to, orreadily developed from known manners, means, techniques and proceduresby practitioners of the chemical, pharmacological, biological,biochemical and medical arts.

It is appreciated that certain features of the invention, which are, forclarity, described in the context of separate embodiments, may also beprovided in combination in a single embodiment. Conversely, variousfeatures of the invention, which are, for brevity, described in thecontext of a single embodiment, may also be provided separately or inany suitable subcombination or as suitable in any other describedembodiment of the invention. Certain features described in the contextof various embodiments are not to be considered essential features ofthose embodiments, unless the embodiment is inoperative without thoseelements.

Various embodiments and aspects of the present invention as delineatedhereinabove and as claimed in the claims section below find experimentalsupport in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with theabove descriptions illustrate some embodiments of the invention in a nonlimiting fashion.

Generally, the nomenclature used herein and the laboratory proceduresutilized in the present invention include molecular, biochemical,microbiological and recombinant DNA techniques. Such techniques arethoroughly explained in the literature. See, for example, “MolecularCloning: A laboratory Manual” Sambrook et al., (1989); “CurrentProtocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed.(1994); Ausubel et al., “Current Protocols in Molecular Biology”, JohnWiley and Sons, Baltimore, Md. (1989); Perbal, “A Practical Guide toMolecular Cloning”, John Wiley & Sons, New York (1988); Watson et al.,“Recombinant DNA”, Scientific American Books, New York; Birren et al.(eds) “Genome Analysis: A Laboratory Manual Series”, Vols. 1-4, ColdSpring Harbor Laboratory Press, New York (1998); methodologies as setforth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and5,272,057; “Cell Biology: A Laboratory Handbook”, Volumes I-III Cellis,J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-IIIColigan J. E., ed. (1994); Stites et al. (eds), “Basic and ClinicalImmunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994);Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”, W.H. Freeman and Co., New York (1980); available immunoassays areextensively described in the patent and scientific literature, see, forexample, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578;3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533;3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521;“Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic AcidHybridization” Hames, B. D., and Higgins S. J., eds. (1985);“Transcription and Translation” Hames, B. D., and Higgins S. J., Eds.(1984); “Animal Cell Culture” Freshney, R. I., ed. (1986); “ImmobilizedCells and Enzymes” IRL Press, (1986); “A Practical Guide to MolecularCloning” Perbal, B., (1984) and “Methods in Enzymology” Vol. 1-317,Academic Press; “PCR Protocols: A Guide To Methods And Applications”,Academic Press, San Diego, Calif. (1990); Marshak et al., “Strategiesfor Protein Purification and Characterization—A Laboratory CourseManual” CSHL Press (1996); all of which are incorporated by reference asif fully set forth herein. Other general references are providedthroughout this document. The procedures therein are believed to be wellknown in the art and are provided for the convenience of the reader. Allthe information contained therein is incorporated herein by reference.

General Experimental and Bioinformatics Methods

RNA extraction—Tissues growing at various growth conditions (asdescribed below) were sampled and RNA was extracted using TRIzol Reagentfrom Invitrogen [Hypertext Transfer Protocol://World Wide Web (dot)invitrogen (dot) com/content (dot)cfm?pageid=469]. Approximately 30-50mg of tissue was taken from samples. The weighed tissues were groundusing pestle and mortar in liquid nitrogen and resuspended in 500 μl ofTRIzol Reagent. To the homogenized lysate, 100 μl of chloroform wasadded followed by precipitation using isopropanol and two washes with75% ethanol. The RNA was eluted in 30 μl of RNase-free water. RNAsamples were cleaned up using Qiagen's RNeasy minikit clean-up protocolas per the manufacturer's protocol (QIAGEN Inc, CA USA). Forconvenience, each micro-array expression information tissue type hasreceived an expression Set ID.

Correlation analysis—was performed for selected genes according to someembodiments of the invention, in which the characterized parameters(measured parameters according to the correlation IDs) were used as “xaxis” for correlation with the tissue transcriptom, which was used asthe “Y axis”. For each gene and measured parameter a correlationcoefficient “R” was calculated (using Pearson correlation) along with ap-value for the significance of the correlation. When the correlationcoefficient (R) between the levels of a gene's expression in a certaintissue and a phenotypic performance across ecotypes/variety/hybrid ishigh in absolute value (between 0.5-1), there is an association betweenthe gene (specifically the expression level of this gene) the phenotypiccharacteristic (e.g., improved nitrogen use efficiency, abiotic stresstolerance, yield, growth rate and the like).

Example I Identifying Genes which Increase Nitrogen Use Efficiency(NUE), Fertilizer Use Efficiency (FUE), Yield, Growth Rate, Vigor,Biomass, Oil Content, Abiotic Stress Tolerance (ABST) and/or Water UseEfficiency (WUE) in Plants

The present inventors have identified polynucleotides which upregulationof expression thereof in plants increases nitrogen use efficiency (NUE),fertilizer use efficiency (FUE), yield (e.g., seed yield, oil yield,biomass, grain quantity and/or quality), growth rate, vigor, biomass,oil content, fiber yield, fiber quality, fiber length, abiotic stresstolerance (ABST) and/or water use efficiency (WUE) of a plant.

All nucleotide sequence datasets used here were originated from publiclyavailable databases or from performing sequencing using the Solexatechnology (e.g. Barley and Sorghum). Sequence data from 100 differentplant species was introduced into a single, comprehensive database.Other information on gene expression, protein annotation, enzymes andpathways were also incorporated. Major databases used include:

Genomes

-   -   Arabidopsis genome [TAIR genome version 6 (Hypertext Transfer        Protocol://World Wide Web (dot) arabidopsis (dot) org/)]    -   Rice genome [IRGSP build 4.0 (Hypertext Transfer Protocol://rgp        (dot) dna (dot) affrc (dot) go (dot) jp/IRGSP/)].    -   Poplar [Populus trichocarpa release 1.1 from JGI (assembly        release v1.0) (Hypertext Transfer Protocol://World Wide Web        (dot) genome (dot) jgi-psf (dot) org/)]    -   Brachypodium [JGI 4x assembly, Hypertext Transfer        Protocol://World Wide Web (dot) brachpodium (dot) org)]    -   Soybean [DOE-JGI SCP, version Glyma0 (Hypertext Transfer        Protocol://World Wide Web (dot) phytozome (dot) net/)]    -   Grape [French-Italian Public Consortium for Grapevine Genome        Characterization grapevine genome (Hypertext Transfer        Protocol://World Wide Web (dot) genoscope (dot) cns (dot) fr/)]    -   Castobean [TIGR/J Craig Venter Institute 4× assembly [(Hypertext        Transfer Protocol://msc (dot) jcvi (dot) org/r_communis]    -   Sorghum [DOE-JGI SCP, version Sbi1 [Hypertext Transfer        Protocol://World Wide Web (dot) phytozome (dot) net/)].    -   Maize [Hypertext Transfer Protocol://maizesequence (dot) org/]    -   Cucumber [Hypertext Transfer Protocol://cucumber (dot) genomics        (dot) org (dot) cn/page/cucumber/index (dot) jsp]    -   Tomato [Hypertext Transfer Protocol://solgenomics (dot)        net/tomato/]    -   Cassava [Hypertext Transfer Protocol://www (dot) phytozome (dot)        net/cassava (dot) php]

Expressed EST and mRNA Sequences were Extracted from the FollowingDatabases:

-   -   GenBank (Hypertext Transfer Protocol://World Wide Web (dot) ncbi        (dot) nlm (dot) nih (dot) gov/Genbank/).    -   RefSeq (Hypertext Transfer Protocol://World Wide Web (dot) ncbi        (dot) nlm (dot) nih (dot) gov/RefSeq/).    -   TAIR (Hypertext Transfer Protocol://World Wide Web (dot)        arabidopsis (dot) org/).

Protein and Pathway Databases

-   -   Uniprot [Hypertext Transfer Protocol://World Wide Web (dot)        uniprot (dot) org/].    -   AraCyc [Hypertext Transfer Protocol://World Wide Web (dot)        arabidopsis (dot) org/biocyc/index (dot) jsp].    -   ENZYME [Hypertext Transfer Protocol://expasy (dot) org/enzyme/].

Microarray Datasets were Downloaded from:

-   -   GEO (Hypertext Transfer Protocol://World Wide        Web.ncbi.nlm.nih.gov/geo/)    -   TAIR (Hypertext Transfer Protocol://World Wide        Web.arabidopsis.org/).    -   Proprietary micro-array data (See WO2008/122980 and Examples        3-10 below).

QTL and SNPs Information

-   -   Gramene [Hypertext Transfer Protocol://World Wide Web (dot)        gramene (dot) org/qtl/].    -   Panzea [Hypertext Transfer Protocol://World Wide Web (dot)        panzea (dot) org/index (dot) html].    -   Soybean QTL: [Hypertext Transfer Protocol://World Wide Web (dot)        soybeanbreederstoolbox(dot) com/].

Database assembly—was performed to build a wide, rich, reliableannotated and easy to analyze database comprised of publicly availablegenomic mRNA, ESTs DNA sequences, data from various crops as well asgene expression, protein annotation and pathway, QTLs data, and otherrelevant information.

Database assembly is comprised of a toolbox of gene refining,structuring, annotation and analysis tools enabling to construct atailored database for each gene discovery project. Gene refining andstructuring tools enable to reliably detect splice variants andantisense transcripts, generating understanding of various potentialphenotypic outcomes of a single gene. The capabilities of the “LEADS”platform of Compugen LTD for analyzing human genome have been confirmedand accepted by the scientific community [see e.g., “WidespreadAntisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21,379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300(5623), 1288-91; “Computational analysis of alternative splicing usingEST tissue information”, Xie H et al. Genomics 2002], and have beenproven most efficient in plant genomics as well.

EST clustering and gene assembly—For gene clustering and assembly oforganisms with available genome sequence data (arabidopsis, rice,castorbean, grape, brachypodium, poplar, soybean, sorghum) the genomicLEADS version (GANG) was employed. This tool allows most accurateclustering of ESTs and mRNA sequences on genome, and predicts genestructure as well as alternative splicing events and anti-sensetranscription.

For organisms with no available full genome sequence data, “expressedLEADS” clustering software was applied.

Gene annotation—Predicted genes and proteins were annotated as follows:Sequences BLAST™ search [Hypertext Transfer Protocol://blast (dot) ncbi(dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] against all plant UniProt[Hypertext Transfer Protocol://World Wide Web (dot) uniprot (dot) org/]was performed. Open reading frames of each putative transcript wereanalyzed and longest ORF with higher number of homologues was selectedas predicted protein of the transcript. The predicted proteins wereanalyzed by InterPro [Hypertext Transfer Protocol://World Wide Web (dot)ebi (dot) ac (dot) uk/interpro/].

BLAST™ against proteins from AraCyc and ENZYME databases was used to mapthe predicted transcripts to AraCyc pathways.

Predicted proteins from different species were compared using BLAST™algorithm [Hypertext Transfer Protocol://World Wide Web (dot) ncbi (dot)nlm (dot) nih (dot) gov/Blast (dot) cgi] to validate the accuracy of thepredicted protein sequence, and for efficient detection of orthologs.

Gene expression profiling—Several data sources were exploited for geneexpression profiling, namely microarray data and digital expressionprofile (see below). According to gene expression profile, a correlationanalysis was performed to identify genes, which are co-regulated underdifferent development stages and environmental conditions and associatedwith different phenotypes.

Publicly available microarray datasets were downloaded from TAIR andNCBI GEO sites, renormalized, and integrated into the database.Expression profiling is one of the most important resource data foridentifying genes important for yield.

A digital expression profile summary was compiled for each clusteraccording to all keywords included in the sequence records comprisingthe cluster. Digital expression, also known as electronic Northern Blot,is a tool that displays virtual expression profile based on the ESTsequences forming the gene cluster. The tool provides the expressionprofile of a cluster in terms of plant anatomy (e.g., the tissue/organin which the gene is expressed), developmental stage (the developmentalstages at which a gene can be found) and profile of treatment (providesthe physiological conditions under which a gene is expressed such asdrought, cold, pathogen infection, etc). Given a random distribution ofESTs in the different clusters, the digital expression provides aprobability value that describes the probability of a cluster having atotal of N ESTs to contain X ESTs from a certain collection oflibraries. For the probability calculations, the following is taken intoconsideration: a) the number of ESTs in the cluster, b) the number ofESTs of the implicated and related libraries, c) the overall number ofESTs available representing the species. Thereby clusters with lowprobability values are highly enriched with ESTs from the group oflibraries of interest indicating a specialized expression.

The accuracy of this system was demonstrated by Portnoy et al., 2009(Analysis Of The Melon Fruit Transcriptome Based On 454 Pyrosequencing)in: Plant & Animal Genomes XVII Conference, San Diego, Calif.Transcriptomic analysis, based on relative EST abundance in data wasperformed by 454 pyrosequencing of cDNA representing mRNA of the melonfruit. Fourteen double strand cDNA samples obtained from two genotypes,two fruit tissues (flesh and rind) and four developmental stages weresequenced. GS FLX pyrosequencing (Roche/454 Life Sciences) ofnon-normalized and purified cDNA samples yielded 1,150,657 expressedsequence tags (ESTs) that assembled into 67,477 unigenes (32,357singletons and 35,120 contigs). Analysis of the data obtained againstthe Cucurbit Genomics Database [Hypertext Transfer Protocol://World WideWeb (dot) icugi (dot) org/] confirmed the accuracy of the sequencing andassembly. Expression patterns of selected genes fitted well theirqRT-PCR data.

Overall, 216 genes were identified to have a major impact on nitrogenuse efficiency, fertilizer use efficiency, yield (e.g., seed yield, oilyield, grain quantity and/or quality), growth rate, vigor, biomass, oilcontent, fiber yield, fiber quality, fiber length, abiotic stresstolerance and/or water use efficiency when expression thereof isincreased in plants. The identified genes, their curated polynucleotideand polypeptide sequences, as well as their updated sequences accordingto GenBank database are summarized in Table 1, hereinbelow.

TABLE 1 Identified polynucleotides for increasing nitrogen useefficiency, fertilizer use efficiency, yield, growth rate, vigor,biomass, oil content, fiber yield, fiber quality, fiber length, abioticstress tolerance and/or water use efficiency of a plant Gene Polyn. SEQPolyp. SEQ ID Name Cluster Name Organism ID NO: NO: LNU290wheat|gb164|BE586041 wheat 1 470 LNU291 sorghum|09v1|BM323576 sorghum 2471 LNU292 sorghum|09v1|SB09G025040 sorghum 3 472 LNU293rice|gb170|OS02G57600 rice 4 473 LNU294 soybean|gb168|BM526182 soybean 5474 LNU295 tomato|09v1|AA824887 tomato 6 475 LNU296rice|gb170|OS05G43380 rice 7 476 LNU297 barley|10v1|AV835353 barley 8477 LNU298 wheat|gb164|BE446740 wheat 9 478 LNU299 maize|gb170|AI622290maize 10 479 LNU300 maize|gb170|AI861194 maize 11 480 LNU301maize|gb170|BM073140 maize 12 481 LNU302 tomato|09v1|BT013543 tomato 13482 LNU303 sorghum|09v1|SB01G004420 sorghum 14 483 LNU304rice|gb170|AU162343 rice 15 484 LNU305 barley|10v1|AV833418 barley 16485 LNU306 arabidopsis|gb165|AT3G03860 arabidopsis 17 486 LNU307maize|gb170|AI941897 maize 18 487 LNU308 arabidopsis|gb165|AT2G14110arabidopsis 19 488 LNU309 millet|09v1|EVO454PM042396 millet 20 489LNU310 tomato|09v1|BG133786 tomato 21 490 LNU311 maize|gb170|CO519241maize 22 491 LNU312 rice|gb170|OS04G53730 rice 23 492 LNU314sorghum|09v1|SB10G001680 sorghum 24 493 LNU315 wheat|gb164|BE497367wheat 25 494 LNU316 sorghum|09v1|SB10G021140 sorghum 26 495 LNU317maize|gb170|CF624079 maize 27 496 LNU318 wheat|gb164|BE443997 wheat 28497 LNU319 sorghum|09v1|SB01G008770 sorghum 29 498 LNU322barley|10v1|BE421151XX1 barley 30 499 LNU323 tomato|09v1|BG123422 tomato31 500 LNU324 sorghum|09v1|SB08G018570 sorghum 32 501 LNU326tomato|09v1|BG126891 tomato 33 502 LNU327 wheat|gb164|CA692356 wheat 34503 LNU328 tomato|09v1|BG128098 tomato 35 504 LNU329tomato|09v1|BG791244 tomato 36 505 LNU330 tomato|09v1|AW096846 tomato 37506 LNU331 tomato|09v1|AW031707 tomato 38 507 LNU332maize|gb170|AW052982 maize 39 508 LNU333 wheat|gb164|BE489159 wheat 40509 LNU335 wheat|gb164|BE500673 wheat 41 510 LNU336 tomato|09v1|AI773791tomato 42 511 LNU337 grape|gb160|CB968839 grape 43 512 LNU339maize|gb170|CB605279 maize 44 513 LNU340 wheat|gb164|BG604469 wheat 45514 LNU341 wheat|gb164|BE490253 wheat 46 515 LNU342 tomato|09v1|BG123334tomato 47 516 LNU343 wheat|gb164|AL825714 wheat 48 517 LNU344wheat|gb164|BJ256846 wheat 49 518 LNU345 wheat|gb164|BF483929 wheat 50519 LNU346 sorghum|09v1|SB09G026910 sorghum 51 520 LNU347sorghum|09v1|SB09G000370 sorghum 52 521 LNU348 maize|gb170|W21614 maize53 522 LNU349 soybean|gb168|CA910292 soybean 54 523 LNU350wheat|gb164|BF201187 wheat 55 524 LNU351 wheat|gb164|BE423861 wheat 56525 LNU352 wheat|gb164|BF474109 wheat 57 526 LNU353 wheat|gb164|BF201797wheat 58 527 LNU354 wheat|gb164|BE445429 wheat 59 528 LNU355wheat|gb164|BF484349 wheat 60 529 LNU356 tomato|09v1|BG629014 tomato 61530 LNU357 tomato|09v1|AI775669 tomato 62 531 LNU359maize|gb170|AI901501 maize 63 532 LNU360 maize|gb170|AI637191 maize 64533 LNU361 maize|gb170|AI612217 maize 65 534 LNU362rice|gb170|OS02G49850 rice 66 535 LNU363 rice|gb170|OS01G59870 rice 67536 LNU364 rice|gb170|OS02G49470 rice 68 537 LNU365rice|gb170|OS04G37820 rice 69 538 LNU366 rice|gb170|OS03G48030 rice 70539 LNU367 rice|gb170|OS02G38970 rice 71 540 LNU368 wheat|gb164|BE490258wheat 72 541 LNU369 wheat|gb164|CA500696 wheat 73 542 LNU370tomato|09v1|AI772811 tomato 74 543 LNU371 maize|gb170|CK985828 maize 75544 LNU372 wheat|gb164|AL825623 wheat 76 545 LNU373rice|gb170|OS12G25200 rice 77 546 LNU374 rice|gb170|OS03G63700 rice 78547 LNU375 tomato|09v1|BG125016 tomato 79 548 LNU376maize|gb170|AW017929 maize 80 549 LNU377 sorghum|09v1|SB01G000775sorghum 81 550 LNU378 wheat|gb164|AJ717146 wheat 82 551 LNU379sorghum|09v1|SB01G015660 sorghum 83 552 LNU380 wheat|gb164|BQ483748wheat 84 553 LNU381 sorghum|09v1|SB04G034690 sorghum 85 554 LNU382arabidopsis|gb165|AT1G65070 arabidopsis 86 555 LNU383tomato|09v1|BG123484 tomato 87 556 LNU384 tomato|09v1|AI482780 tomato 88557 LNU385 rice|gb170|OS01G25600 rice 89 558 LNU386rice|gb170|OS06G35200 rice 90 559 LNU387 sorghum|09v1|SB02G032450sorghum 91 560 LNU388 rice|gb170|OS04G58410 rice 92 561 LNU390tomato|09v1|BG125049 tomato 93 562 LNU391 barley|10v1|BE060369 barley 94563 LNU392 rice|gb170|OS03G11420 rice 95 564 LNU393sorghum|09v1|SB04G005560 sorghum 96 565 LNU395 sorghum|09v1|SB06G025090sorghum 97 566 LNU396 sorghum|09v1|SB01G048410 sorghum 98 567 LNU397sorghum|09v1|SB03G031230 sorghum 99 568 LNU399 wheat|gb164|CA655009wheat 100 569 LNU401 sorghum|09v1|SB04G002180 sorghum 101 570 LNU402wheat|gb164|CK212389 wheat 102 571 LNU403 sorghum|09v1|SB03G041600sorghum 103 572 LNU405 tomato|09v1|BG125067 tomato 104 573 LNU407barley|10v1|AJ484347 barley 105 574 LNU408 barley|10v1|BE421189 barley106 575 LNU409 barley|10v1|GH227248 barley 107 576 LNU410wheat|gb164|BE424655 wheat 108 577 LNU411 tomato|09v1|BI207068 tomato109 578 LNU412 cotton|gb164|BE053302 cotton 110 579 LNU413tomato|09v1|BG126757 tomato 111 580 LNU414 wheat|gb164|CA653735 wheat112 581 LNU415 sorghum|09v1|SB01G048990 sorghum 113 582 LNU416b_juncea|gb164|EVGN00464924783313 b_juncea 114 583 LNU417wheat|gb164|BG607934 wheat 115 584 LNU419 tomato|09v1|BG132251 tomato116 585 LNU420 sorghum|09v1|SB01G040070 sorghum 117 586 LNU421sorghum|09v1|SB06G031090 sorghum 118 587 LNU422 sorghum|09v1|SB07G002970sorghum 119 588 LNU423 sorghum|09v1|SB01G001120 sorghum 120 589 LNU424arabidopsis|gb165|AT5G02240 arabidopsis 121 590 LNU425barley|10v1|AJ461142 barley 122 591 LNU426 rice|gb170|OS06G48320 rice123 592 LNU427 rice|gb170|OS03G03140 rice 124 593 LNU429tomato|09v1|BG124215 tomato 125 594 LNU430 tomato|09v1|BG130012 tomato126 595 LNU431 sorghum|09v1|SB10G024110 sorghum 127 596 LNU432sorghum|09v1|SB03G013220 sorghum 128 597 LNU433 sorghum|09v1|SB04G026690sorghum 129 598 LNU434 sorghum|09v1|SB01G046460 sorghum 130 599 LNU435barley|10v1|BE060935 barley 131 600 LNU436 barley|10v1|BE422114 barley132 601 LNU437 barley|10v1|BI950410 barley 133 602 LNU438barley|10v1|BE437298 barley 134 603 LNU439 sorghum|09v1|SB09G005970sorghum 135 604 LNU441 sorghum|09v1|SB01G037770 sorghum 136 605 LNU442tomato|09v1|AW735755 tomato 137 606 LNU443 brachypodium|09v1|GT769494brachypodium 138 607 LNU444 cotton|gb164|AI726042 cotton 139 608 LNU445soybean|gb168|FK341642 soybean 140 609 LNU446 soybean|gb168|BE917590soybean 141 610 LNU447 barley|10v1|BF254963 barley 142 611 LNU448barley|10v1|BE422325 barley 143 612 LNU449 cotton|gb164|AI725388 cotton144 613 LNU450 cotton|gb164|AI728722 cotton 145 614 LNU451tomato|09v1|BG124246 tomato 146 615 LNU453 sorghum|09v1|SB10G027420sorghum 147 616 LNU454 tomato|09v1|BG127794 tomato 148 617 LNU455tomato|09v1|BG626661 tomato 149 618 LNU456 barley|10v1|BF265366 barley150 619 LNU457 tomato|gb164|CK714827 tomato 151 620 LNU458cotton|10v1|DW508164 cotton 152 621 LNU459 maize|gb170|BM350702 maize153 622 LNU460 maize|gb170|AW066359 maize 154 623 LNU461tomato|09v1|AI483350 tomato 155 624 LNU462 tomato|09v1|AI896771 tomato156 625 LNU463 grape|gb160|CB346636 grape 157 626 LNU464grape|gb160|CB968657 grape 158 627 LNU465 sorghum|09v1|SB03G033750sorghum 159 628 LNU466 barley|10v1|AV833763 barley 160 629 LNU467barley|10v1|BF254449 barley 161 630 LNU468 tomato|09v1|AI637280 tomato162 631 LNU469 maize|gb170|BI542994 maize 163 632 LNU470barley|10v1|BQ760445 barley 164 633 LNU471 maize|gb170|BQ035243 maize165 634 LNU472 barley|10v1|BI780920 barley 166 635 LNU473sorghum|09v1|SB03G013160 sorghum 167 636 LNU474 soybean|gb168|CV536461soybean 168 637 LNU476 maize|gb170|AW400216 maize 169 638 LNU477sorghum|09v1|SB01G035950 sorghum 170 639 LNU479 sorghum|09v1|SB01G011640sorghum 171 640 LNU480 sorghum|09v1|SB01G003380 sorghum 172 641 LNU481sorghum|09v1|SB01G045180 sorghum 173 642 LNU482 cotton|10v1|BF273404cotton 174 643 LNU483 rice|gb170|OS02G49880 rice 175 644 LNU485rice|gb170|OS04G52230 rice 176 645 LNU486 rice|gb170|OS08G04560 rice 177646 LNU489 tomato|09v1|BG132312 tomato 178 647 LNU490poplar|10v1|CA822678 poplar 179 648 LNU491 sorghum|09v1|SB01G031120sorghum 180 649 LNU492 rice|gb170|OS07G46790 rice 181 650 LNU493rice|gb170|OS06G34040 rice 182 651 LNU494 maize|gb170|BE186249 maize 183652 LNU495 sorghum|09v1|SB03G028760 sorghum 184 653 LNU496wheat|gb164|CA640674 wheat 185 654 LNU497 wheat|gb164|BE516527 wheat 186655 LNU498 sorghum|09v1|SB02G002830 sorghum 187 656 LNU499barley|10v1|AV923755 barley 188 657 LNU500 tomato|09v1|BG643024 tomato189 658 LNU501 sorghum|09v1|SB10G026500 sorghum 190 659 LNU502barley|10v1|BI958006 barley 191 660 LNU503 rice|gb170|OS04G52300 rice192 661 LNU504 arabidopsis|gb165|AT2G19110 arabidopsis 193 662 LNU506tomato|09v1|AI490778 tomato 194 663 LNU507 barley|10v1|BF621023 barley195 664 LNU508 rice|gb170|AA753097 rice 196 665 LNU509rice|gb170|OS01G21990 rice 197 666 LNU510 rice|gb170|OS06G29844 rice 198667 LNU511 rice|gb170|OS03G48260 rice 199 668 LNU512arabidopsis|gb165|AT1G54040 arabidopsis 200 669 LNU513soybean|gb168|BE822210 soybean 201 670 LNU514 rice|gb170|BE040128 rice202 671 LNU517 soybean|gb168|AW201968 soybean 203 672 LNU518maize|gb170|CA404810 maize 204 673 LNU519 maize|gb170|CF046227 maize 205674 LNU520 sorghum|09v1|SB10G027140 sorghum 206 675 LNU309_H3maize|gb170|AW165565 maize 207 676 LNU417_H4 maize|10v1|CB381339 maize208 677 LNU431_H1 maize|10v1|CO528919 maize 209 678 LNU437_H2rice|gb170|OS11G37700 rice 210 679 LNU313 sorghum|09v1|CF757586 sorghum211 — LNU358 maize|gb170|AI615229 maize 212 — LNU394maize|gb170|AI491593 maize 213 — LNU418 maize|gb170|AW165449 maize 214 —LNU487 barley|10v1|AJ475337 barley 215 — LNU488 barley|10v1|AJ469759barley 216 — LNU410 wheat|gb164|BE424655 wheat 108 699 LNU504arabidopsis|gb165|AT2G19110 arabidopsis 193 712 LNU487barley|10v1|AJ475337 barley 215 708 LNU290 wheat|gb164|BE586041 wheat217 680 LNU292 sorghum|09v1|SB09G025040 sorghum 218 472 LNU294soybean|gb168|BM526182 soybean 219 681 LNU297 barley|10v1|AV835353barley 220 682 LNU300 maize|gb170|AI861194 maize 221 683 LNU309millet|09v1|EVO454PM042396 millet 222 684 LNU312 rice|gb170|OS04G53730rice 223 492 LNU314 sorghum|09v1|SB10G001680 sorghum 224 685 LNU332maize|gb170|AW052982 maize 225 508 LNU337 grape|gb160|CB968839 grape 226686 LNU341 wheat|gb164|BE490253 wheat 227 687 LNU350wheat|gb164|BF201187 wheat 228 688 LNU353 wheat|gb164|BF201797 wheat 229689 LNU364 rice|gb170|OS02G49470 rice 230 537 LNU368wheat|gb164|BE490258 wheat 231 690 LNU369 wheat|gb164|CA500696 wheat 232691 LNU372 wheat|gb164|AL825623 wheat 233 692 LNU378wheat|gb164|AJ717146 wheat 234 693 LNU378 wheat|gb164|AJ717146 wheat 235694 LNU380 wheat|gb164|BQ483748 wheat 236 695 LNU381sorghum|09v1|SB04G034690 sorghum 237 554 LNU382arabidopsis|gb165|AT1G65070 arabidopsis 238 555 LNU393sorghum|09v1|SB04G005560 sorghum 239 565 LNU401 sorghum|09v1|SB04G002180sorghum 240 696 LNU407 barley|10v1|AJ484347 barley 241 697 LNU409barley|10v1|GH227248 barley 242 698 LNU414 wheat|gb164|CA653735 wheat243 700 LNU416 b_juncea|gb164|EVGN00464924783313 b_juncea 244 701 LNU417wheat|gb164|BG607934 wheat 245 702 LNU433 sorghum|09v1|SB04G026690sorghum 246 598 LNU443 brachypodium|09v1|GT769494 brachypodium 247 607LNU447 barley|10v1|BF254963 barley 248 611 LNU453sorghum|09v1|SB10G027420 sorghum 249 703 LNU454 tomato|09v1|BG127794tomato 250 617 LNU457 tomato|gb164|CK714827 tomato 251 704 LNU466barley|10v1|AV833763 barley 252 705 LNU470 barley|10v1|BQ760445 barley253 706 LNU474 soybean|gb168|CV536461 soybean 254 707 LNU488barley|10v1|AJ469759 barley 255 709 LNU490 poplar|10v1|CA822678 poplar256 648 LNU495 sorghum|09v1|SB03G028760 sorghum 257 710 LNU500tomato|09v1|BG643024 tomato 258 711 LNU506 tomato|09v1|AI490778 tomato259 713 LNU508 rice|gb170|AA753097 rice 260 714 LNU509rice|gb170|OS01G21990 rice 261 666 LNU309_H3 maize|gb170|AW165565 maize262 715 LNU431_H1 maize|gb170|CO528919 maize 263 716 LNU313sorghum|09v1|CF757586 sorghum 264 — LNU358 maize|gb170|AI615229 maize265 — Table 1. Provided are the identified genes along with theirsequence identifiers. “Polyp.” = polypeptide; “Polyn.”—Polynucleotide.

Example 2 Identification of Homologous Sequences that Increase NitrogenUse Efficiency, Fertilizer Use Efficiency, Yield, Growth Rate, Vigor,Biomass, Oil Content, Abiotic Stress Tolerance and/or Water UseEfficiency in Plants

The concepts of orthology and paralogy have recently been applied tofunctional characterizations and classifications on the scale ofwhole-genome comparisons. Orthologs and paralogs constitute two majortypes of homologs: The first evolved from a common ancestor byspecialization, and the latter is related by duplication events. It isassumed that paralogs arising from ancient duplication events are likelyto have diverged in function while true orthologs are more likely toretain identical function over evolutionary time.

To further investigate and identify putative orthologs of the genesaffecting nitrogen use efficiency, fertilizer use efficiency, yield(e.g., seed yield, oil yield, biomass, grain quantity and/or quality),growth rate, vigor, biomass, oil content, abiotic stress toleranceand/or water use efficiency, all sequences were aligned using the BLAST™(/Basic Local Alignment Search Tool/). Sequences sufficiently similarwere tentatively grouped. These putative orthologs were furtherorganized under a Phylogram—a branching diagram (tree) assumed to be arepresentation of the evolutionary relationships among the biologicaltaxa. Putative ortholog groups were analyzed as to their agreement withthe phylogram and in cases of disagreements these ortholog groups werebroken accordingly. Expression data was analyzed and the EST librarieswere classified using a fixed vocabulary of custom terms such asdevelopmental stages (e.g., genes showing similar expression profilethrough development with up regulation at specific stage, such as at theseed filling stage) and/or plant organ (e.g., genes showing similarexpression profile across their organs with up regulation at specificorgans such as seed). The annotations from all the ESTs clustered to agene were analyzed statistically by comparing their frequency in thecluster versus their abundance in the database, allowing theconstruction of a numeric and graphic expression profile of that gene,which is termed “digital expression”. The rationale of using these twocomplementary methods with methods of phenotypic association studies ofQTLs, SNPs and phenotype expression correlation is based on theassumption that true orthologs are likely to retain identical functionover evolutionary time. These methods provide different sets ofindications on function similarities between two homologous genes,similarities in the sequence level—identical amino acids in the proteindomains and similarity in expression profiles.

The search and identification of homologous genes involves the screeningof sequence information available, for example, in public databases,which include but are not limited to the DNA Database of Japan (DDBJ),Genbank, and the European Molecular Biology Laboratory Nucleic AcidSequence Database (EMBL) or versions thereof or the MIPS database. Anumber of different search algorithms have been developed, including butnot limited to the suite of programs referred to as BLAST™ programs.There are five implementations of BLAST™, three designed for nucleotidesequence queries (BLASTN™, BLASTX™, and TBLASTX™) and two designed forprotein sequence queries (BLASTP™ and TBLASTN™) (Coulson, Trends inBiotechnology: 76-80, 1994; Birren et al., Genome Analysis, I: 543,1997). Such methods involve alignment and comparison of sequences. TheBLAST™ algorithm calculates percent sequence identity and performs astatistical analysis of the similarity between the two sequences. Thesoftware for performing BLAST™ analysis is publicly available throughthe National Centre for Biotechnology Information. Other such softwareor algorithms are GAP, BESTFIT, FASTA and TFASTA. GAP uses the algorithmof Needleman and Wunsch (J. Mol. Biol. 48: 443-453, 1970) to find thealignment of two complete sequences that maximizes the number of matchesand minimizes the number of gaps.

The homologous genes may belong to the same gene family. The analysis ofa gene family may be carried out using sequence similarity analysis. Toperform this analysis one may use standard programs for multiplealignments e.g. Clustal W. A neighbor-joining tree of the proteinshomologous to the genes of some embodiments of the invention may be usedto provide an overview of structural and ancestral relationships.Sequence identity may be calculated using an alignment program asdescribed above. It is expected that other plants will carry a similarfunctional gene (orthologue) or a family of similar genes and thosegenes will provide the same preferred phenotype as the genes presentedhere. Advantageously, these family members may be useful in the methodsof some embodiments of the invention. Example of other plants include,but not limited to, barley (Hordeum vulgare), Arabidopsis (Arabidopsisthaliana), maize (Zea mays), cotton (Gossypium), Oilseed rape (Brassicanapus), Rice (Oryza sativa), Sugar cane (Saccharum officinarum), Sorghum(Sorghum bicolor), Soybean (Glycine max), Sunflower (Helianthus annuus),Tomato (Lycopersicon esculentum) and Wheat (Triticum aestivum).

The above-mentioned analyses for sequence homology is preferably carriedout on a full-length sequence, but may also be based on a comparison ofcertain regions such as conserved domains. The identification of suchdomains, would also be well within the realm of the person skilled inthe art and would involve, for example, a computer readable format ofthe nucleic acids of some embodiments of the invention, the use ofalignment software programs and the use of publicly availableinformation on protein domains, conserved motifs and boxes. Thisinformation is available in the PRODOM (Hypertext TransferProtocol://World Wide Web (dot) biochem (dot) ucl (dot) ac (dot)uk/bsm/dbbrowser/protocol/prodomqry (dot) html), PIR (Hypertext TransferProtocol://pir (dot) Georgetown (dot) edu/) or Pfam (Hypertext TransferProtocol://World Wide Web (dot) sanger (dot) ac (dot) uk/Software/Pfam/)database. Sequence analysis programs designed for motif searching may beused for identification of fragments, regions and conserved domains asmentioned above. Preferred computer programs include, but are notlimited to, MEME, SIGNALSCAN, and GENESCAN.

A person skilled in the art may use the homologous sequences providedherein to find similar sequences in other species and other organisms.Homologues of a protein encompass, peptides, oligopeptides,polypeptides, proteins and enzymes having amino acid substitutions,deletions and/or insertions relative to the unmodified protein inquestion and having similar biological and functional activity as theunmodified protein from which they are derived. To produce suchhomologues, amino acids of the protein may be replaced by other aminoacids having similar properties (conservative changes, such as similarhydrophobicity, hydrophilicity, antigenicity, propensity to form orbreak a-helical structures or 3-sheet structures). Conservativesubstitution Tables are well known in the art [see for example Creighton(1984) Proteins. W.H. Freeman and Company]. Homologues of a nucleic acidencompass nucleic acids having nucleotide substitutions, deletionsand/or insertions relative to the unmodified nucleic acid in questionand having similar biological and functional activity as the unmodifiednucleic acid from which they are derived.

Polynucleotides and polypeptides with significant homology to theidentified genes described in Table 1 (Example 1 above) were identifiedfrom the databases using BLAST™ software using the BLASTP™ and TBLASTN™algorithms. The query polypeptide sequences were SEQ ID NOs: 470-716(which are encoded by the polynucleotides SEQ ID NOs:1-265, shown inTable 1 above) and SEQ ID NOs:717-784 (which are encoded by the clonedgenes SEQ ID NOs:266-469, shown in Table 68 (Example 13, below) and theidentified homologous sequences are provided in Table 2, below.

TABLE 2 Homologues of the identified genes/polypeptides for increasingnitrogen use efficiency, fertilizer use efficiency, yield, seed yield,growth rate, vigor, biomass, oil content, fiber yield, fiber quality,fiber length, abiotic stress tolerance and/or water use efficiency of aplant Hom. Polyn. Polyp. to SEQ Hom. to SEQ SEQ % ID Gene ID ID globalNO: Name cluster name NO: NO: identity Algor. 785 LNU290leymus|gb166|EG374697_P1 2398 470 89.8 globlastp 786 LNU290wheat|10v2|BE499260_P1 2399 470 81 globlastp 787 LNU290barley|10v2|BF624085_P1 2400 470 80.8 globlastp 788 LNU290oat|10v2|GR316625_P1 2401 470 80.2 globlastp 789 LNU291maize|gb170|CF035629 471 471 100 globlastp 790 LNU291sugarcane|10v1|GFXAE009947X12 471 471 100 globlastp 791 LNU291maize|10v1|EG151714_P1 2402 471 98.5 globlastp 792 LNU291maize|gb170|CRPZM2N041615 2402 471 98.5 globlastp 793 LNU291maize|10v1|DW738796_P1 2403 471 98 globlastp 794 LNU291maize|gb170|DW809324 2403 471 98 globlastp 795 LNU291rice|gb170|OS04G16738 2404 471 97.01 glotblastn 796 LNU291rice|gb170|OSP1G00360 2405 471 97 globlastp 797 LNU291wheat|10v2|GFXWHTCPPSBGX1_T1 — 471 96.02 glotblastn 798 LNU291barley|10v1|BJ463973 2406 471 95.52 glotblastn 799 LNU291barley|10v2|BJ463973_P1 2407 471 95.5 globlastp 800 LNU291brachypodium|09v1|GFXEU325680X11_P1 2408 471 95.5 globlastp 801 LNU291brachypodium|09v1|CRPBD014715_T1 2409 471 94.53 glotblastn 802 LNU291lolium|10v1|GFXAM777385X11_T1 2410 471 94.03 glotblastn 803 LNU291maize|10v1|DW746358_P1 2411 471 90 globlastp 804 LNU291maize|gb170|CRPZM2N041741 2411 471 90 globlastp 805 LNU291maize|10v1|DW898492_P1 2412 471 85.1 globlastp 806 LNU291maize|gb170|CRPZM2N087668 2412 471 85.1 globlastp 807 LNU291banana|10v1|GFXEU017022X1_P1 2413 471 84.1 globlastp 807 LNU302banana|10v1|GFXEU017022X1_P1 2413 482 86.1 globlastp 808 LNU291poppy|gb166|FE967418_T1 2414 471 80.6 glotblastn 808 LNU302poppy|gb166|FE967418_T1 2414 482 91.04 glotblastn 809 LNU291arabidopsis_lyrata|09v1|JGIAL006450_P1 2415 471 80.1 globlastp 809LNU302 arabidopsis_lyrata|09v1|JGIAL006450_P1 2415 482 88.1 globlastp810 LNU292 maize|10v1|AI855230_T1 2416 472 93.33 glotblastn 811 LNU292maize|gb170|AI855230 2417 472 93.3 globlastp 812 LNU292maize|10v1|AI629623_P1 2418 472 89.8 globlastp 813 LNU292millet|10v1|EVO454PM068054_P1 2419 472 83.7 globlastp 814 LNU295solanum_phureja|09v1|SPHAA824887 2420 475 97.7 globlastp 815 LNU295eggplant|10v1|FS032066_P1 2421 475 95 globlastp 816 LNU295petunia|gb171|CV300743_P1 2422 475 94.6 globlastp 817 LNU295nicotiana_benthamiana|gb162| 2423 475 94.1 globlastp EH366260_P1 818LNU295 pepper|gb171|AF082717_P1 2424 475 94.1 globlastp 819 LNU295aquilegia|10v1|DR939800 2425 475 86.4 globlastp 820 LNU295aquilegia|10v2|DR939800_P1 2425 475 86.4 globlastp 821 LNU295coffea|10v1|CF588912_P1 2426 475 85.5 globlastp 822 LNU295onion|gb162|CF450542_P1 2427 475 84.6 globlastp 823 LNU295kiwi|gb166|FG406602_P1 2428 475 82.8 globlastp 824 LNU295papaya|gb165|EX249843_P1 2429 475 82.8 globlastp 825 LNU295citrus|gb166|CD575353_P1 2430 475 82.4 globlastp 826 LNU295apple|gb171|CN493682_P1 2431 475 81.9 globlastp 827 LNU295oak|10v1|FP026569_P1 2432 475 81 globlastp 828 LNU295cleome_spinosa|10v1|GR935187_P1 2433 475 81 globlastp 829 LNU295ipomoea_nil|10v1|BJ553751_P1 2434 475 80.6 globlastp 830 LNU295peanut|10v1|CD037840_P1 2435 475 80.5 globlastp 830 LNU299peanut|10v1|CD037840_P1 2435 479 80.1 globlastp 831 LNU295avocado|10v1|CK754477_P1 2436 475 80.5 globlastp 832 LNU295nasturtium|10v1|SRR032558S0015258_P1 2437 475 80.5 globlastp 833 LNU295peanut|gb171|CD037840 2435 475 80.5 globlastp 833 LNU299peanut|gb171|CD037840 2435 479 80.1 globlastp 834 LNU295prunus|10v1|CB823956_P1 2438 475 80.1 globlastp 834 LNU299prunus|10v1|CB823956_P1 2438 479 80.5 globlastp 835 LNU295b_rapa|gb162|CX272134_P1 2439 475 80.1 globlastp 836 LNU295cotton|10v1|AI726608 2440 475 80.1 globlastp 837 LNU295cotton|10v2|BE053131_P1 2441 475 80.1 globlastp 838 LNU295thellungiella|gb167|BY803571 2442 475 80.1 globlastp 839 LNU295prunus|gb167|CB823956 2438 475 80.1 globlastp 839 LNU299prunus|gb167|CB823956 2438 479 80.5 globlastp 840 LNU295grape|gb160|BM436999_T1 2443 475 80.09 glotblastn 841 LNU298wheat|gb164|BF483176 2444 478 91.8 globlastp 842 LNU298wheat|10v2|CA678180_P1 2445 478 89.2 globlastp 843 LNU298wheat|gb164|BE500660 2445 478 89.2 globlastp 844 LNU298barley|10v1|AV832797 2446 478 85.9 globlastp 845 LNU298barley|10v2|AV832797_P1 2446 478 85.9 globlastp 846 LNU299sorghum|09v1|SB03G006050 2447 479 97.3 globlastp 847 LNU299sugarcane|10v1|BQ535654 2448 479 95.9 globlastp 848 LNU299foxtail_millet|10v2|SICRP019205_P1 2449 479 90.5 globlastp 849 LNU299rice|gb170|OS04G20280 2450 479 88.2 globlastp 850 LNU299cenchrus|gb166|EB654614_P1 2451 479 87.4 globlastp 851 LNU299cynodon|10v1|ES292284_P1 2452 479 86.4 globlastp 852 LNU299rice|gb170|OS01G05694 2453 479 86 globlastp 853 LNU299millet|10v1|EVO454PM008366_P1 2454 479 85.1 globlastp 854 LNU299brachypodium|09v1|GT782155_P1 2455 479 83.8 globlastp 855 LNU299switchgrass|gb167|DN148482 2456 479 83.71 glotblastn 856 LNU299switchgrass|gb167|DN152162 2457 479 83.3 globlastp 857 LNU299wheat|gb164|BE404513 2458 479 82.4 globlastp 858 LNU299wheat|10v2|BE404513_P1 2458 479 82.4 globlastp 859 LNU299wheat|gb164|BF203016 2459 479 82.4 globlastp 860 LNU299pseudoroegneria|gb167|FF352036 2460 479 81.9 globlastp 861 LNU299wheat|gb164|BE414418 2461 479 81 globlastp 862 LNU299wheat|10v2|BE414418_P1 2461 479 81 globlastp 863 LNU299barley|10v2|AJ461592_P1 2462 479 80.5 globlastp 864 LNU299lovegrass|gb167|EH184276_P1 2463 479 80.5 globlastp 865 LNU300maize|10v1|T18817_P1 2464 480 98.5 globlastp 866 LNU300sorghum|09v1|SB09G004320_P1 2465 480 98.3 globlastp 867 LNU300sugarcane|10v1|CA065017_P1 2466 480 98.3 globlastp 868 LNU300foxtail_millet|10v2|OXEC612314T1_P1 2467 480 95.1 globlastp 869 LNU300millet|10v1|CD725150_P1 2468 480 93.8 globlastp 870 LNU300switchgrass|gb167|FE621296_P1 2469 480 90.4 globlastp 871 LNU300rice|gb170|OS05G06350_P1 2470 480 89.9 globlastp 872 LNU300brachypodium|09v1|DV470466_P1 2471 480 89.1 globlastp 873 LNU300barley|10v2|BE413102_P1 2472 480 88.8 globlastp 874 LNU300wheat|10v2|BE400103_P1 2473 480 88.6 globlastp 875 LNU300oat|10v2|CN815116_P1 2474 480 88 globlastp 876 LNU300cassava|09v1|JGICASSAVA12817VALIDM1_P1 2475 480 82.7 globlastp 877LNU300 cassava|09v1|DV441758_P1 2476 480 82 globlastp 878 LNU300cacao|10v1|CU476740_P1 2477 480 81.4 globlastp 879 LNU300centaurea|gb166|EH712147_P1 2478 480 81.4 globlastp 880 LNU300castorbean|09v1|XM002512439_P1 2479 480 81.1 globlastp 881 LNU300sequoia|10v1|SRR065044S0000578_P1 2480 480 81.1 globlastp 882 LNU300eucalyptus|11v1|CD668810_P1 2481 480 81 globlastp 883 LNU300podocarpus|10v1|SRR065014S0001157_P1 2482 480 80.9 globlastp 884 LNU300aristolochia|10v1|SRR039082S0002761_P1 2483 480 80.7 globlastp 885LNU300 cotton|10v2|CO071731_P1 2484 480 80.6 globlastp 886 LNU300melon|10v1|DV631718_P1 2485 480 80.6 globlastp 887 LNU300poplar|10v1|BI070314_P1 2486 480 80.6 globlastp 888 LNU300abies|11v1|SRR098676X100270_P1 2487 480 80.5 globlastp 889 LNU300pine|10v2|AA556627_P1 2488 480 80.5 globlastp 890 LNU300taxus|10v1|SRR032523S0008792_T1 2489 480 80.34 glotblastn 891 LNU300aquilegia|10v2|DR928227_P1 2490 480 80.2 globlastp 892 LNU300cucumber|09v1|DN909459_P1 2491 480 80.2 globlastp 893 LNU300poplar|10v1|AI165556_P1 2492 480 80.2 globlastp 894 LNU300lettuce|10v1|DW046351_T1 2493 480 80.19 glotblastn 895 LNU300eucalyptus|11v1|CD668073_P1 2494 480 80.1 globlastp 896 LNU300melon|10v1|AM728431_P1 2495 480 80.1 globlastp 897 LNU300spurge|gb161|BI961995_P1 2496 480 80.1 globlastp 898 LNU300pseudotsuga|10v1|SRR065119S0006823_P1 2497 480 80 globlastp 899 LNU300soybean|11v1|GLYMA10G29000_P1 2498 480 80 globlastp 900 LNU300soybean|11v1|GLYMA17G03430_P1 2499 480 80 globlastp 901 LNU300soybean|11v1|GLYMA20G38320_P1 2500 480 80 globlastp 902 LNU300spruce|gb162|CO216885_P1 2501 480 80 globlastp 903 LNU301maize|gb170|LLBE049863 2502 481 93.02 glotblastn 904 LNU301sugarcane|10v1|BQ533050 2503 481 92.3 globlastp 905 LNU301switchgrass|gb167|FL742623 2504 481 92.2 globlastp 906 LNU301sorghum|09v1|SB06G026660 2505 481 90.8 globlastp 907 LNU301switchgrass|gb167|FL879708 2506 481 90.6 globlastp 908 LNU301foxtail_millet|10v2|SICRP008244_P1 2507 481 88.4 globlastp 909 LNU301millet|10v1|EVO454PM138026_P1 2508 481 87.5 globlastp 910 LNU301cenchrus|gb166|EB660401_P1 2509 481 86.8 globlastp 911 LNU302solanum_phureja|09v1|SPHBG126319 2510 482 99.5 globlastp 912 LNU302solanum_phureja|09v1|SPHAW216568 2511 482 98.5 globlastp 913 LNU302guizotia|10v1|GE556119_T1 2512 482 95.02 glotblastn 914 LNU302coffea|10v1|GFXEF044213X12_P1 2513 482 95 globlastp 915 LNU302tragopogon|10v1|SRR020205S0004258 2514 482 94.5 globlastp 916 LNU302parthenium|10v1|GFXGU120098X5_P1 2515 482 94 globlastp 917 LNU302lettuce|10v1|GFXAP007232X13_P1 2516 482 94 globlastp 918 LNU302artemisia|10v1|SRR019254S0016920_T1 2517 482 93.53 glotblastn 919 LNU302sunflower|gb162|CD854704 2518 482 93.5 globlastp 920 LNU302sunflower|10v1|CD854108_P1 2518 482 93.5 globlastp 921 LNU302cassava|09v1|GFXEU117376X11_P1 2519 482 93 globlastp 922 LNU302dandelion|10v1|DR400271_T1 2520 482 92.54 glotblastn 923 LNU302castorbean|09v1|SRR020784S0000611_P1 2521 482 92 globlastp 924 LNU302ginseng|10v1|GFXAY582139X12_P1 2522 482 92 globlastp 925 LNU302prunus|gb167|AJ873078 2523 482 91.5 globlastp 926 LNU302potato|10v1|BQ116812_P1 2524 482 91 globlastp 927 LNU302oak|10v1|GFXGQ998723X1_T1 — 482 90.59 glotblastn 928 LNU302peanut|10v1|EG030533_T1 2525 482 90.55 glotblastn 929 LNU302oak|gb170|SRR006307S0026883 2526 482 90.1 globlastp 930 LNU302grape|gb160|BM437168_T1 2527 482 90.05 glotblastn 931 LNU302lotus|09v1|CRPLJ002102_T1 2528 482 90.05 glotblastn 932 LNU302walnuts|gb166|EL892734 2529 482 90.05 glotblastn 933 LNU302cotton|10v2|GFXAP009123X10_T1 — 482 90.05 glotblastn 934 LNU302cotton|10v1|GFXAP009123X11 2530 482 90 globlastp 935 LNU302grape|gb160|CD717918_P1 2531 482 90 globlastp 936 LNU302lotus|09v1|CRPLJ004552_P1 2532 482 90 globlastp 937 LNU302medicago|09v1|BI271493_P1 2533 482 90 globlastp 938 LNU302lotus|09v1|CRPLJ033270_P1 2534 482 89.6 globlastp 939 LNU302oak|10v1|GFXAF132888X1_T1 — 482 89.6 glotblastn 940 LNU302medicago|09v1|CRPMT030772_T1 2535 482 89.55 glotblastn 941 LNU302canola|10v1|H07661_T1 2536 482 89.05 glotblastn 942 LNU302citrus|gb166|BQ624493_T1 2537 482 89.05 glotblastn 943 LNU302radish|gb164|EV526475 2538 482 89.05 glotblastn 944 LNU302acacia|10v1|FS585044_T1 — 482 88.67 glotblastn 945 LNU302arabidopsis_lyrata|09v1|JGIAL006381_T1 2539 482 88.56 glotblastn 946LNU302 arabidopsis|10v1|ATCG00380_P1 2540 482 88.1 globlastp 947 LNU302strawberry|11v1|SRR034865S0051981_T1 2541 482 88.06 glotblastn 948LNU302 aristolochia|10v1|GFXAF528920X1_T1 — 482 88.06 glotblastn 949LNU302 pigeonpea|10v1|GW346536XX1_P1 2542 482 87.1 globlastp 950 LNU302avocado|10v1|CK766348_P1 2543 482 87.1 globlastp 951 LNU302castorbean|09v1|CRPRC006998_T1 2544 482 87.06 glotblastn 952 LNU302soybean|gb168|BE940860 2545 482 87.06 glotblastn 953 LNU302amborella|gb166|CD482397_P1 2546 482 86.1 globlastp 954 LNU302lotus|09v1|CRPLJ009646_P1 2547 482 85.2 globlastp 955 LNU302orobanche|10v1|GFXAJ007723X1_P1 2548 482 84.7 globlastp 956 LNU302bean|gb167|CA903466_P1 2549 482 84.1 globlastp 957 LNU302pigeonpea|10v1|SRR054580S0061346_T1 2550 482 84.08 glotblastn 958 LNU302soybean|11v1|CRPGM014792_T1 2551 482 83.58 glotblastn 959 LNU302soybean|gb168|GD329396 2551 482 83.58 glotblastn 960 LNU302sunflower|gb162|CD854693 2552 482 83.17 glotblastn 961 LNU302iceplant|gb164|CA834888_P1 2553 482 82.8 globlastp 962 LNU302zostera|10v1|AM768670_P1 2554 482 82.1 globlastp 963 LNU302lotus|09v1|CRPLJ011938_P1 2555 482 82.1 globlastp 964 LNU302nuphar|gb166|FD384632_P1 2556 482 81.6 globlastp 965 LNU302lotus|09v1|CRPLJ040445_T1 2557 482 81.09 glotblastn 966 LNU302solanum_phureja|09v1|SPHCRPSP004055 2558 482 80.6 glotblastn 967 LNU302b_oleracea|gb161|DY019834_T1 2559 482 80.1 glotblastn 968 LNU303sugarcane|10v1|CA076623 2560 483 96.3 globlastp 969 LNU303maize|gb170|LLAF055471 2561 483 92 globlastp 970 LNU303maize|10v1|CB280860_P1 2562 483 88 globlastp 971 LNU303millet|10v1|EVO454PM042955_P1 2563 483 85.8 globlastp 972 LNU303foxtail_millet|10v2|EC613241_P1 2564 483 82.9 globlastp 973 LNU304switchgrass|gb167|FE613746 2565 484 85.4 globlastp 974 LNU304foxtail_millet|10v2|SICRP021546_P1 2566 484 84.4 globlastp 975 LNU304millet|10v1|EVO454PM063553_P1 2567 484 83.3 globlastp 976 LNU304millet|09v1|EVO454PM063553 2567 484 83.3 globlastp 977 LNU304sorghum|09v1|SB10G020830 2568 484 83.2 globlastp 978 LNU304sugarcane|10v1|BQ533360 2569 484 83.2 globlastp 979 LNU304cenchrus|gb166|BM084119_P1 2570 484 82.3 globlastp 980 LNU304maize|gb170|LLBM335916 2571 484 82.3 globlastp 981 LNU304maize|gb170|LLAI855177 2572 484 82.1 globlastp 982 LNU304wheat|10v2|BG604828_P1 2573 484 81.1 globlastp 983 LNU304wheat|gb164|BG604828 2573 484 81.1 globlastp 984 LNU304cynodon|10v1|ES294050_P1 2574 484 80.6 globlastp 985 LNU304maize|10v1|BM501421_P1 2575 484 80 globlastp 986 LNU304maize|gb170|BM501421 2576 484 80 globlastp 987 LNU304wheat|10v2|BF478734_P1 2577 484 80 globlastp 988 LNU304wheat|gb164|BF478734 2577 484 80 globlastp 989 LNU304wheat|10v2|CA620694_P1 2577 484 80 globlastp 990 LNU304wheat|gb164|CA620694 2577 484 80 globlastp 991 LNU305wheat|10v2|BE429958_P1 2578 485 90.5 globlastp 992 LNU305wheat|gb164|BE429958 2579 485 89.8 globlastp 993 LNU305pseudoroegneria|gb167|FF355165 2580 485 87.5 globlastp 994 LNU305leymus|gb166|EG401835_P1 2581 485 80.4 globlastp 995 LNU306arabidopsis_lyrata|09v1|JGIAL008724_P1 2582 486 95 globlastp 996 LNU306radish|gb164|EV545889 2583 486 83.4 globlastp 997 LNU306canola|10v1|EV004258_P1 2584 486 82.5 globlastp 998 LNU307sorghum|09v1|SB04G000775 2585 487 81.46 glotblastn 999 LNU308arabidopsis_lyrata|09v1|JGIAL011758_P1 2586 488 97.9 globlastp 1000LNU308 thellungiella|gb167|BY803273 2587 488 91.6 globlastp 1001 LNU308radish|gb164|EX749633 2588 488 91.1 globlastp 1002 LNU308radish|gb164|EX750313 2589 488 91.1 globlastp 1003 LNU308canola|10v1|CD822987_P1 2590 488 88.9 globlastp 1004 LNU308b_oleracea|gb161|DY030174_P1 2591 488 88.4 globlastp 1005 LNU308canola|10v1|EE465545_P1 2591 488 88.4 globlastp 1006 LNU308cleome_spinosa|10v1|GR931012_P1 2592 488 84.7 globlastp 1007 LNU309sorghum|09v1|SB03G036080 2593 489 85.6 glotblastn 1007 LNU309_H3sorghum|09v1|SB03G036080 2593 676 92.3 globlastp 1008 LNU309foxtail_millet|10v2|SICRP027522_T1 2594 489 83.12 glotblastn 1008LNU309_H3 foxtail_millet|10v2|SICRP027522_T1 2594 715 85.71 glotblastn1009 LNU309 brachypodium|09v1|DV479613_T1 2595 489 80.03 glotblastn 1009LNU309_H3 brachypodium|09v1|DV479613_T1 2595 715 84.76 glotblastn 1010LNU311 sugarcane|10v1|CA183153 2596 491 80.95 glotblastn 1011 LNU315pseudoroegneria|gb167|FF344590 2597 494 98.1 globlastp 1012 LNU315wheat|gb164|BE604654 2598 494 97.5 globlastp 1013 LNU315foxtail_millet|10v2|FXTSLX00113403D1_P1 2599 494 96.2 globlastp 1014LNU315 wheat|10v2|CA598385_P1 2600 494 95.6 globlastp 1015 LNU315wheat|10v2|CJ898820_P1 2601 494 94.9 globlastp 1016 LNU315wheat|gb164|BE606227 2601 494 94.9 globlastp 1017 LNU315barley|10v1|BE420626 2602 494 91.9 globlastp 1018 LNU315barley|10v2|BE420626_P1 2602 494 91.9 globlastp 1019 LNU315wheat|10v2|DR735055_T1 2603 494 87.97 glotblastn 1020 LNU315wheat|gb164|DR735055 2603 494 87.97 glotblastn 1021 LNU317sorghum|09v1|SB09G020280 2604 496 85.8 globlastp 1022 LNU318wheat|10v2|BE406534_P1 2605 497 97.6 globlastp 1023 LNU318wheat|gb164|BE406534 2605 497 97.6 globlastp 1024 LNU318leymus|gb166|EG378119_P1 2606 497 95.9 globlastp 1025 LNU318wheat|10v2|CA602663_P1 2607 497 94.3 globlastp 1026 LNU318wheat|gb164|CA602663 2607 497 94.3 globlastp 1027 LNU318barley|10v1|BE412753 2608 497 92.7 globlastp 1028 LNU318barley|10v2|BE412753_P1 2608 497 92.7 globlastp 1029 LNU318oat|10v1|GO587598 2609 497 86.2 globlastp 1030 LNU318oat|10v2|GR319951_P1 2610 497 85.4 globlastp 1031 LNU318oat|10v2|GR332951_P1 2611 497 84.7 globlastp 1032 LNU318oat|10v1|GR319951 2611 497 84.7 globlastp 1033 LNU318brachypodium|09v1|GT768729_P1 2612 497 82.1 globlastp 1034 LNU319sugarcane|10v1|CA070744 2613 498 97.7 globlastp 1035 LNU319switchgrass|gb167|FL790597 2614 498 94.2 globlastp 1036 LNU319maize|10v1|AW052987_P1 2615 498 93.1 globlastp 1037 LNU319switchgrass|gb167|FE614987 2616 498 93.1 globlastp 1038 LNU319millet|10v1|EVO454PM019765_P1 2617 498 90.8 globlastp 1039 LNU319rice|gb170|OS03G52730 2618 498 87.9 globlastp 1040 LNU319foxtail_millet|10v2|FXTRMSLX00487607D1_P1 2619 498 87.1 globlastp 1041LNU319 oat|10v2|SRR020741S0030408_P1 2620 498 85 globlastp 1042 LNU319oat|10v1|CN815589 2621 498 85 globlastp 1043 LNU319 wheat|gb164|CA6598832622 498 84.7 globlastp 1044 LNU319 brachypodium|09v1|DV486914_P1 2623498 83.8 globlastp 1045 LNU319 wheat|gb164|BG312713 2624 498 83.5globlastp 1046 LNU319 wheat|gb164|CA697520 2625 498 83.5 globlastp 1047LNU319 wheat|10v2|BG312713_P1 2626 498 83.5 globlastp 1048 LNU319barley|10v2|BF621514_P1 2627 498 81.6 globlastp 1049 LNU322wheat|10v2|BE426358_P1 2628 499 95.2 globlastp 1050 LNU322wheat|gb164|BE426358 2628 499 95.2 globlastp 1051 LNU322wheat|10v2|BF293712_T1 2629 499 88.27 glotblastn 1052 LNU322oat|10v2|CN817938_P1 2630 499 82.6 globlastp 1053 LNU322oat|10v1|GR329806 2630 499 82.6 globlastp 1054 LNU322brachypodium|09v1|GT790865_P1 2631 499 81.9 globlastp 1054 LNU420brachypodium|09v1|GT790865_P1 2631 586 80.5 globlastp 1055 LNU324maize|10v1|BE122952_P1 2632 501 95.5 globlastp 1056 LNU324maize|gb170|BE122952 2632 501 95.5 globlastp 1057 LNU324maize|gb170|AI600531 2633 501 93.5 globlastp 1058 LNU324maize|10v1|AI600531_P1 2633 501 93.5 globlastp 1059 LNU324switchgrass|gb167|FE601004 2634 501 91 globlastp 1060 LNU324maize|gb170|LLAY104119 2635 501 88.98 glotblastn 1061 LNU324millet|10v1|CD726160_P1 2636 501 88.4 globlastp 1062 LNU324brachypodium|09v1|GT764807_P1 2637 501 87.1 globlastp 1063 LNU324oat|10v2|GR352457_P1 2638 501 86.8 globlastp 1064 LNU324wheat|10v2|BE429701_P1 2639 501 85.7 globlastp 1065 LNU324wheat|gb164|BE429701 2640 501 85.7 globlastp 1066 LNU324rice|gb170|OS12G37960 2641 501 85.4 globlastp 1067 LNU324barley|10v2|BF625656_P1 2642 501 85.1 globlastp 1068 LNU324leymus|gb166|EG374667_T1 2643 501 84.79 glotblastn 1069 LNU324pseudoroegneria|gb167|FF341068 2644 501 83.1 globlastp 1070 LNU327wheat|10v2|CV770918_P1 2645 503 97.1 globlastp 1071 LNU327barley|10v1|BG415996 2646 503 95.2 globlastp 1072 LNU327barley|10v2|BG415996_P1 2646 503 95.2 globlastp 1073 LNU328solanum_phureja|09v1|SPHBG128098 2647 504 89.6 globlastp 1074 LNU330solanum_phureja|09v1|SPHAW096846 2648 506 97.4 globlastp 1075 LNU330potato|10v1|AW096846_P1 2649 506 96.6 globlastp 1076 LNU330pepper|gb171|GD057272_P1 2650 506 81.1 globlastp 1077 LNU330tobacco|gb162|AM786444 2651 506 80.79 glotblastn 1078 LNU331solanum_phureja|09v1|SPHAW031707 2652 507 96.5 globlastp 1079 LNU331potato|10v1|BQ519367_P1 2653 507 95.9 globlastp 1080 LNU332sorghum|09v1|SB03G028460 2654 508 92.6 globlastp 1081 LNU332maize|gb170|AW231427 2655 508 90.8 globlastp 1082 LNU332maize|10v1|AW231427_P1 2656 508 90.6 globlastp 1083 LNU332rice|gb170|OS01G43580 2657 508 84.8 globlastp 1084 LNU332brachypodium|09v1|GT760062_T1 2658 508 81.44 glotblastn 1085 LNU333wheat|10v2|BE418424_P1 2659 509 94.6 globlastp 1086 LNU333wheat|gb164|BE418424 2659 509 94.6 globlastp 1087 LNU333wheat|10v2|BI751337_P1 2660 509 91.2 globlastp 1088 LNU333wheat|gb164|BI751337 2660 509 91.2 globlastp 1089 LNU333pseudoroegneria|gb167|FF342941 2661 509 89.1 globlastp 1090 LNU333barley|10v1|BF621665 2662 509 88.1 globlastp 1091 LNU333barley|10v2|BF621665_P1 2662 509 88.1 globlastp 1092 LNU333oat|10v2|GR329274_P1 2663 509 80.3 globlastp 1093 LNU333oat|10v1|GR329274 2663 509 80.3 globlastp 1094 LNU335wheat|gb164|BF483351 2664 510 97 globlastp 1095 LNU335barley|10v1|BI951100 2665 510 91.2 globlastp 1096 LNU335barley|10v2|BI951100_P1 2665 510 91.2 globlastp 1097 LNU335brachypodium|09v1|GT787495_P1 2666 510 83.5 globlastp 1098 LNU335wheat|10v2|BF202225_T1 2667 510 82.22 glotblastn 1099 LNU336solanum_phureja|09v1|SPHAI773791 2668 511 98.6 globlastp 1100 LNU336tobacco|gb162|AB003038 2669 511 95.2 globlastp 1101 LNU337cassava|09v1|JGICASSAVA31518VALIDM1_P1 2670 512 84.9 globlastp 1102LNU337 cacao|10v1|CU502884_P1 2671 512 83.4 globlastp 1103 LNU337castorbean|09v1|EG671098_P1 2672 512 83.4 globlastp 1104 LNU337clementine|11v1|CV885061_P1 2673 512 82.3 globlastp 1105 LNU337orange|11v1|CV885061_P1 2674 512 82.3 globlastp 1106 LNU337strawberry|11v1|EX671413_P1 2675 512 82 globlastp 1107 LNU337oak|10v1|DN949924_P1 2676 512 81.2 globlastp 1108 LNU337prunus|10v1|CN494497_P1 2677 512 81.2 globlastp 1109 LNU337cotton|10v2|SRR032368S0318405_P1 2678 512 80.2 globlastp 1110 LNU337cotton|10v1|CO105456 2679 512 80.1 globlastp 1111 LNU337poplar|10v1|CX170984_P1 2680 512 80 globlastp 1112 LNU340barley|10v1|AJ476977 2681 514 99.6 globlastp 1113 LNU340barley|10v2|AJ476977_P1 2681 514 99.6 globlastp 1114 LNU340oat|10v2|CN820997_P1 2682 514 92.1 globlastp 1115 LNU340brachypodium|09v1|GT772953_P1 2683 514 87.4 globlastp 1116 LNU340rice|gb170|OS12G02380 2684 514 82.2 globlastp 1117 LNU340rice|gb170|OS11G02450 2685 514 81.7 globlastp 1118 LNU341leymus|gb166|EG382663_P1 2686 515 88.2 globlastp 1119 LNU342potato|10v1|BI176929_P1 2687 516 94.3 globlastp 1120 LNU342solanum_phureja|09v1|SPHBG123334 2688 516 94 globlastp 1121 LNU342eggplant|10v1|FS005150_P1 2689 516 88.6 globlastp 1122 LNU342tobacco|gb162|DW000438 2690 516 85.8 globlastp 1123 LNU342pepper|gb171|BM064975_P1 2691 516 85.5 globlastp 1124 LNU343barley|10v2|BF624427_P1 2692 517 99.2 globlastp 1125 LNU343pseudoroegneria|gb167|FF354777 2693 517 98.5 globlastp 1126 LNU343wheat|10v2|SRR043332S0002679_P1 2694 517 97 globlastp 1127 LNU343wheat|gb164|AL822523 2694 517 97 globlastp 1128 LNU343oat|10v2|SRR020741S0012373_P1 2695 517 88 globlastp 1129 LNU344barley|10v1|AV922746 2696 518 97.1 globlastp 1129 LNU347barley|10v1|AV922746 2696 521 81.4 globlastp 1130 LNU344barley|10v1|BE437694 2696 518 97.1 globlastp 1130 LNU347barley|10v1|BE437694 2696 521 81.4 globlastp 1131 LNU344brachypodium|09v1|GT770285_P1 2696 518 97.1 globlastp 1131 LNU347brachypodium|09v1|GT770285_P1 2696 521 81.4 globlastp 1132 LNU344oat|10v1|SRR020741S0153418 2696 518 97.1 globlastp 1132 LNU347oat|10v1|SRR020741S0153418 2696 521 81.4 globlastp 1133 LNU344wheat|10v2|BE404009_P1 2696 518 97.1 globlastp 1133 LNU347wheat|10v2|BE404009_P1 2696 521 81.4 globlastp 1134 LNU344wheat|gb164|BE404009 2696 518 97.1 globlastp 1134 LNU347wheat|gb164|BE404009 2696 521 81.4 globlastp 1135 LNU344wheat|10v2|BE605093_P1 2696 518 97.1 globlastp 1135 LNU347wheat|10v2|BE605093_P1 2696 521 81.4 globlastp 1136 LNU344wheat|gb164|BE605093 2696 518 97.1 globlastp 1136 LNU347wheat|gb164|BE605093 2696 521 81.4 globlastp 1137 LNU344wheat|gb164|CA627002 2696 518 97.1 globlastp 1137 LNU347wheat|gb164|CA627002 2696 521 81.4 globlastp 1138 LNU344barley|10v2|BE437694_P1 2696 518 97.1 globlastp 1138 LNU347barley|10v2|BE437694_P1 2696 521 81.4 globlastp 1139 LNU344oat|10v2|GR334207_P1 2697 518 95.7 globlastp 1139 LNU347oat|10v2|GR334207_P1 2697 521 80 globlastp 1140 LNU344 oat|10v1|GR3342072697 518 95.7 globlastp 1140 LNU347 oat|10v1|GR334207 2697 521 80globlastp 1141 LNU344 fescue|gb161|DT700305_P1 2698 518 94.2 globlastp1141 LNU347 fescue|gb161|DT700305_P1 2698 521 81.4 globlastp 1142 LNU344rye|gb164|BE637285 2699 518 91.3 glotblastn 1143 LNU344rice|gb170|OS05G01290 2700 518 87 globlastp 1143 LNU347rice|gb170|OS05G01290 2700 521 88.6 globlastp 1144 LNU344foxtail_millet|10v2|FXTSLX00736715D2_T1 2701 518 85.51 glotblastn 1145LNU344 cynodon|10v1|ES296145_P1 2702 518 84.3 globlastp 1145 LNU347cynodon|10v1|ES296145_P1 2702 521 87.1 globlastp 1146 LNU344foxtail_millet|10v2|FXTRMSLX00208339D1_P1 2703 518 81.4 globlastp 1146LNU347 foxtail_millet|10v2|FXTRMSLX00208339D1_P1 2703 521 88.6 globlastp1147 LNU345 wheat|gb164|BG604995 2704 519 95.7 globlastp 1148 LNU345wheat|10v2|BG604995_P1 2705 519 94.9 globlastp 1149 LNU345barley|10v1|BI954292 2706 519 92.2 globlastp 1150 LNU345barley|10v2|BI954292_P1 2706 519 92.2 globlastp 1151 LNU345pseudoroegneria|gb167|FF342688 2707 519 88.8 globlastp 1152 LNU345wheat|gb164|CA719534 2708 519 87.1 globlastp 1153 LNU345leymus|gb166|EG386923_T1 2709 519 82.64 glotblastn 1154 LNU346sugarcane|10v1|CA067223 2710 520 96.3 globlastp 1155 LNU346maize|10v1|AI676894_P1 2711 520 94.9 globlastp 1156 LNU346maize|gb170|LLAI676894 2711 520 94.9 globlastp 1157 LNU346maize|10v1|AI677358_P1 2712 520 92.5 globlastp 1158 LNU346maize|gb170|AI677358 2713 520 92.5 globlastp 1159 LNU346foxtail_millet|10v2|SICRP019925_P1 2714 520 91.2 globlastp 1160 LNU346millet|10v1|EVO454PM028709_P1 2715 520 90.4 globlastp 1161 LNU346switchgrass|gb167|FE610979 2716 520 90.1 globlastp 1162 LNU346rice|gb170|OS05G46230 2717 520 85.9 globlastp 1163 LNU346brachypodium|09v1|GT773225_P1 2718 520 84.6 globlastp 1164 LNU346oat|10v2|GO586894_P1 2719 520 82.9 globlastp 1165 LNU346oat|10v1|GO586894 2719 520 82.9 globlastp 1166 LNU346wheat|10v2|BQ238470_P1 2720 520 82.8 globlastp 1167 LNU346wheat|gb164|BE400556 2720 520 82.8 globlastp 1168 LNU346leymus|gb166|EG381704_P1 2721 520 82.1 globlastp 1169 LNU346wheat|gb164|BQ238470 2722 520 80.64 glotblastn 1170 LNU346millet|09v1|CD726327 2723 520 80 glotblastn 1171 LNU347maize|gb170|LLFL008896 2724 521 92.9 globlastp 1172 LNU347maize|gb170|BG836075 2725 521 91.4 globlastp 1173 LNU347maize|10v1|BG836075_P1 2725 521 91.4 globlastp 1174 LNU347millet|09v1|EVO454PM007718 2726 521 84.3 globlastp 1175 LNU347millet|10v1|EVO454PM007718_P1 2726 521 84.3 globlastp 1176 LNU347switchgrass|gb167|FL737420 2727 521 82.86 glotblastn 1177 LNU348sugarcane|10v1|CA103796 2728 522 87.9 globlastp 1178 LNU348maize|10v1|EU942853_P1 2729 522 85.6 globlastp 1179 LNU348maize|gb170|EU942853 2729 522 85.6 globlastp 1180 LNU348sorghum|09v1|SB03G009900 2730 522 85.5 globlastp 1181 LNU348foxtail_millet|10v2|SICRP040741_P1 2731 522 82.4 globlastp 1182 LNU348millet|10v1|PMSLX0001425D2_P1 2732 522 81 globlastp 1183 LNU349bean|gb167|BQ481480_P1 2733 523 96 globlastp 1184 LNU349soybean|11v1|GLYMA15G06990_P1 2734 523 96 globlastp 1185 LNU349soybean|gb168|AW687261 2734 523 96 globlastp 1186 LNU349pigeonpea|10v1|SRR054580S0126664_P1 2735 523 93.8 globlastp 1187 LNU349cowpea|gb166|FF395146_P1 2736 523 93 globlastp 1188 LNU349liquorice|gb171|FS268558_P1 2737 523 83.4 globlastp 1189 LNU349peanut|10v1|GO260668_P1 2738 523 83.2 globlastp 1190 LNU349peanut|gb171|ES752840 2739 523 83.2 globlastp 1191 LNU349lotus|09v1|LLBW601593_P1 2740 523 81.2 globlastp 1192 LNU351barley|10v1|BI948837 2741 525 98 globlastp 1193 LNU351barley|10v2|BI948837_P1 2741 525 98 globlastp 1194 LNU351wheat|10v2|BE419429_P1 2742 525 97.2 globlastp 1195 LNU351wheat|gb164|BE419429 2742 525 97.2 globlastp 1196 LNU351oat|10v2|CN818075_P1 2743 525 95.7 globlastp 1197 LNU351oat|10v1|CN818075 2743 525 95.7 globlastp 1198 LNU351fescue|gb161|DT694710_P1 2744 525 94.6 globlastp 1199 LNU351brachypodium|09v1|DV473443_P1 2745 525 92.2 globlastp 1200 LNU351cynodon|10v1|ES299286_P1 2746 525 90.7 globlastp 1201 LNU351millet|10v1|EVO454PM006850_P1 2747 525 89.9 globlastp 1201 LNU424millet|10v1|EVO454PM006850_T1 2747 590 80.24 glotblastn 1202 LNU351foxtail_millet|10v2|OXFXTSLX00031185D1T1_P1 2748 525 89.5 globlastp 1203LNU351 sugarcane|10v1|CA119908 2749 525 89.5 globlastp 1203 LNU424sugarcane|10v1|CA119908 2749 590 81.03 glotblastn 1204 LNU351switchgrass|gb167|FL735769 2750 525 89.1 globlastp 1205 LNU351sorghum|09v1|SB01G003100 2751 525 88.7 globlastp 1206 LNU351cenchrus|gb166|EB657417_P1 2752 525 88.3 globlastp 1207 LNU351pseudoroegneria|gb167|FF361410 2753 525 88.2 globlastp 1208 LNU351rice|gb170|OS03G60740 2754 525 87.9 globlastp 1209 LNU351wheat|gb164|BE425410 2755 525 87.6 globlastp 1210 LNU351maize|10v1|AA979757_P1 2756 525 87.5 globlastp 1211 LNU351maize|gb170|AA979757 2756 525 87.5 globlastp 1212 LNU351millet|09v1|EVO454PM006850 2757 525 86.6 globlastp 1213 LNU351switchgrass|gb167|DN145627 2758 525 85.6 globlastp 1214 LNU351melon|10v1|AM724047_P1 2759 525 85.5 globlastp 1214 LNU424melon|10v1|AM724047_P1 2759 590 81.6 globlastp 1215 LNU351pigeonpea|10v1|GW351947_P1 2760 525 85.1 globlastp 1216 LNU351cucumber|09v1|CV004115_P1 2761 525 84.8 globlastp 1216 LNU424cucumber|09v1|CV004115_P1 2761 590 81.2 globlastp 1217 LNU351cassava|09v1|DV444815_P1 2762 525 83.5 globlastp 1218 LNU351lotus|09v1|LLBI419507_P1 2763 525 83.1 globlastp 1219 LNU351medicago|09v1|LLAL374329_P1 2764 525 83.1 globlastp 1220 LNU351peanut|10v1|EE125913_P1 2765 525 82.7 globlastp 1221 LNU351peanut|10v1|ES703043_P1 2766 525 82.7 globlastp 1222 LNU351pepper|gb171|BM061311_P1 2767 525 82.7 globlastp 1222 LNU424pepper|gb171|BM061311_P1 2767 590 80.8 globlastp 1223 LNU351melon|gb165|AM724047 2768 525 82.68 glotblastn 1224 LNU351ginger|gb164|DY345448_P1 2769 525 82.4 globlastp 1225 LNU351peanut|gb171|EC365304 2770 525 82.4 globlastp 1226 LNU351chestnut|gb170|SRR006295S0003362_P1 2771 525 82.4 globlastp 1226 LNU424chestnut|gb170|SRR006295S0003362_P1 2771 590 80.8 globlastp 1227 LNU351potato|10v1|BE921048_P1 2772 525 82.4 globlastp 1227 LNU424potato|10v1|BE921048_P1 2772 590 80 globlastp 1228 LNU351solanum_phureja|09v1|SPHAI484349 2772 525 82.4 globlastp 1228 LNU424solanum_phureja|09v1|SPHAI484349 2772 590 80 globlastp 1229 LNU351cleome_gynandra|10v1|SRR015532S0006049_T1 2773 525 82.28 glotblastn 1229LNU424 cleome_gynandra|10v1|SRR015532S0006049_T1 2773 590 80.63glotblastn 1230 LNU351 cleome_spinosa|10v1|SRR015531S0002685_T1 2774 52582.28 glotblastn 1230 LNU424 cleome_spinosa|10v1|SRR015531S0002685_T12774 590 80.63 glotblastn 1231 LNU351 eggplant|10v1|FS009160_P1 2775 52582 globlastp 1232 LNU351 sunflower|10v1|DY916239_P1 2776 525 82globlastp 1233 LNU351 sunflower|gb162|DY916239 2776 525 82 globlastp1234 LNU351 eucalyptus|11v1|CD669334_P1 2777 525 82 globlastp 1235LNU351 cassava|09v1|CK644785_P1 2778 525 82 globlastp 1235 LNU424cassava|09v1|CK644785_P1 2778 590 81.6 globlastp 1236 LNU351aristolochia|10v1|SRR039082S0026666_P1 2779 525 81.6 globlastp 1237LNU351 artemisia|10v1|EY044641_P1 2780 525 81.6 globlastp 1238 LNU351eucalyptus|gb166|CD669334 2781 525 81.6 globlastp 1239 LNU351oak|10v1|CU657816_P1 2782 525 81.6 globlastp 1240 LNU351oak|gb170|CU657816 2783 525 81.6 globlastp 1240 LNU424oak|gb170|CU657816 2783 590 80.4 globlastp 1241 LNU351canola|10v1|BQ704593_T1 2784 525 81.57 glotblastn 1242 LNU351petunia|gb171|CV296541_T1 2785 525 81.57 glotblastn 1243 LNU351radish|gb164|EV535078 2786 525 81.57 glotblastn 1244 LNU351cowpea|gb166|FF382538_P1 2787 525 81.2 globlastp 1245 LNU351tomato|09v1|BG130491 2788 525 81.2 globlastp 1246 LNU351nasturtium|10v1|GH170446_P1 2789 525 81.2 globlastp 1246 LNU424nasturtium|10v1|GH170446_P1 2789 590 81.6 globlastp 1247 LNU351b_rapa|gb162|CA991656_T1 2790 525 81.18 glotblastn 1248 LNU351clementine|11v1|BQ623383_T1 2791 525 81.1 glotblastn 1248 LNU424clementine|11v1|BQ623383_T1 2791 590 81.03 glotblastn 1249 LNU351cleome_spinosa|10v1|SRR015531S0011482_P1 2792 525 81.1 globlastp 1249LNU424 cleome_spinosa|10v1|SRR015531S0011482_P1 2792 590 87 globlastp1250 LNU351 cotton|10v1|AI727053 2793 525 81.1 globlastp 1250 LNU424cotton|10v1|AI727053 2793 590 80.2 globlastp 1251 LNU351cotton|10v2|BF276321_T1 2794 525 81.1 glotblastn 1251 LNU424cotton|10v2|BF276321_T1 2794 590 80.63 glotblastn 1252 LNU351tobacco|gb162|DV162696 2795 525 80.8 globlastp 1253 LNU351centaurea|gb166|EH725206_P1 2796 525 80.8 globlastp 1253 LNU424centaurea|gb166|EH725206_P1 2796 590 80 globlastp 1254 LNU351cichorium|gb171|DT212712_P1 2797 525 80.8 globlastp 1254 LNU424cichorium|gb171|DT212712_P1 2797 590 80.4 globlastp 1255 LNU351canola|10v1|CB686246_T1 2798 525 80.78 glotblastn 1256 LNU351aquilegia|10v1|DR918778 2799 525 80.71 glotblastn 1257 LNU351aquilegia|10v2|DR918778_T1 2800 525 80.71 glotblastn 1258 LNU351poplar|10v1|BU867914_T1 2801 525 80.71 glotblastn 1259 LNU351citrus|gb166|BQ623383_T1 2802 525 80.71 glotblastn 1259 LNU424citrus|gb166|BQ623383_T1 2802 590 80.63 glotblastn 1260 LNU351prunus|10v1|CB821110_P1 2803 525 80.4 globlastp 1260 LNU424prunus|10v1|CB821110_P1 2803 590 80.4 globlastp 1261 LNU351castorbean|09v1|EE255183_P1 2804 525 80.4 globlastp 1261 LNU424castorbean|09v1|EE255183_P1 2804 590 80 globlastp 1262 LNU351tragopogon|10v1|SRR020205S0033542 2805 525 80.4 globlastp 1262 LNU424tragopogon|10v1|SRR020205S0033542 2805 590 80.24 glotblastn 1263 LNU351orange|11v1|BQ623383_T1 2806 525 80.31 glotblastn 1263 LNU424orange|11v1|BQ623383_T1 2806 590 80.24 glotblastn 1264 LNU351soybean|11v1|GLYMA07G15960_P1 2807 525 80.3 globlastp 1265 LNU351soybean|gb168|AW349893 2807 525 80.3 globlastp 1266 LNU351radish|gb164|EV548023 2808 525 80.3 globlastp 1266 LNU424radish|gb164|EV548023 2808 590 94.5 globlastp 1267 LNU351artemisia|10v1|SRR019254S0015151_P1 2809 525 80 globlastp 1268 LNU351avocado|10v1|CK756872_T1 2810 525 80 glotblastn 1269 LNU351b_juncea|gb164|EVGN00219108490673 2811 525 80 glotblastn 1270 LNU351cowpea|gb166|FC458138_P1 2812 525 80 globlastp 1271 LNU351tea|10v1|CV014405 2813 525 80 glotblastn 1272 LNU351apple|gb171|CN494974_P1 2814 525 80 globlastp 1272 LNU424apple|gb171|CN494974_P1 2814 590 80 globlastp 1273 LNU351coffea|10v1|DV673538_T1 2815 525 80 glotblastn 1273 LNU424coffea|10v1|DV673538_T1 2815 590 80.39 glotblastn 1274 LNU351nasturtium|10v1|SRR032558S0065877_P1 2816 525 80 globlastp 1274 LNU424nasturtium|10v1|SRR032558S0065877_P1 2816 590 80.8 globlastp 1275 LNU351prunus|gb167|CB821110 2817 525 80 globlastp 1275 LNU424prunus|gb167|CB821110 2817 590 80 globlastp 1276 LNU352wheat|gb164|BE352575 2818 526 99.5 globlastp 1277 LNU352wheat|gb164|CA647188 2819 526 99.3 globlastp 1278 LNU352barley|10v1|BI947860 2820 526 98.6 globlastp 1279 LNU352barley|10v2|BI947860_P1 2820 526 98.6 globlastp 1280 LNU352brachypodium|09v1|DV477609_P1 2821 526 93.1 globlastp 1281 LNU352rice|gb170|OS07G04690 2822 526 90.5 globlastp 1282 LNU352cenchrus|gb166|BM084107_P1 2823 526 89.6 globlastp 1283 LNU352switchgrass|gb167|DN143189 2824 526 89.6 globlastp 1284 LNU352millet|10v1|EVO454PM016009_P1 2825 526 89.1 globlastp 1285 LNU352sugarcane|10v1|CA096470 2826 526 86.5 globlastp 1286 LNU352sorghum|09v1|SB02G002660 2827 526 86 globlastp 1287 LNU352fescue|gb161|DT699878_T1 2828 526 80.33 glotblastn 1288 LNU353brachypodium|09v1|GT820624_T1 2829 527 82.27 glotblastn 1289 LNU353rice|gb170|OS09G26870_P1 2830 527 80.4 globlastp 1290 LNU354barley|10v1|BE060054 2831 528 91.3 globlastp 1291 LNU354barley|10v2|BE060054_P1 2831 528 91.3 globlastp 1292 LNU354wheat|10v2|CK212438_P1 2832 528 89.9 globlastp 1293 LNU354wheat|gb164|CK212438 2833 528 89.86 glotblastn 1294 LNU354wheat|gb164|BG314359 2834 528 84.9 globlastp 1295 LNU354wheat|10v2|BE518059_P1 2835 528 84.1 globlastp 1296 LNU354wheat|10v2|BE426361_P1 2836 528 83.6 globlastp 1297 LNU354wheat|gb164|BE426361 2836 528 83.6 globlastp 1298 LNU354wheat|gb164|BE518059 2837 528 82.6 globlastp 1299 LNU354barley|10v1|BJ449982 2838 528 80.3 globlastp 1300 LNU354barley|10v2|BJ449982_P1 2838 528 80.3 globlastp 1301 LNU355wheat|gb164|BE426518 2839 529 97.1 globlastp 1302 LNU355wheat|gb164|BF200864 2840 529 96.8 globlastp 1303 LNU355pseudoroegneria|gb167|FF340622 2841 529 96.1 globlastp 1304 LNU355leymus|gb166|EG375848_P1 2842 529 95.1 globlastp 1305 LNU355barley|10v1|BF628570 2843 529 92.9 globlastp 1306 LNU355barley|10v2|BF628570_P1 2843 529 92.9 globlastp 1307 LNU355rice|gb170|OS05G38230 2844 529 83 globlastp 1308 LNU355cynodon|10v1|ES295926_T1 2845 529 81.35 glotblastn 1309 LNU355sorghum|09v1|SB09G022370 2846 529 80.39 glotblastn 1310 LNU356solanum_phureja|09v1|SPHBG631091 2847 530 93.6 globlastp 1311 LNU356pepper|gb171|GD057444_P1 2848 530 86 globlastp 1312 LNU357solanum_phureja|09v1|SPHAI775669 2849 531 98.6 globlastp 1313 LNU357potato|10v1|BM112538_P1 2850 531 98.3 globlastp 1314 LNU357pepper|gb171|BM064560_P1 2851 531 91 globlastp 1315 LNU357eggplant|10v1|FS005730_P1 2852 531 90.7 globlastp 1316 LNU357tobacco|gb162|CV018003 2853 531 88.6 globlastp 1317 LNU357potato|10v1|BG350748_P1 2854 531 87.2 globlastp 1318 LNU357solanum_phureja|09v1|SPHAF225512 2854 531 87.2 globlastp 1319 LNU357tomato|09v1|AF225512 2855 531 87.2 globlastp 1320 LNU357petunia|gb171|CV294419_P1 2856 531 86.5 globlastp 1321 LNU357tobacco|gb162|EB445511 2857 531 83.4 globlastp 1322 LNU357triphysaria|10v1|BE574853 2858 531 83.4 globlastp 1323 LNU357triphysaria|10v1|EY165458 2859 531 83 globlastp 1324 LNU357ipomoea_nil|10v1|BJ555173_P1 2860 531 82.4 globlastp 1325 LNU357orobanche|10v1|SRR023189S0021630_P1 2861 531 82.4 globlastp 1326 LNU357monkeyflower|10v1|CV521906_P1 2862 531 80.6 globlastp 1327 LNU359sorghum|09v1|SB03G007230 2863 532 96.2 globlastp 1328 LNU359millet|10v1|EB410926_T1 2864 532 90.72 glotblastn 1329 LNU359rice|gb170|OS01G03950 2865 532 87.6 globlastp 1330 LNU359wheat|10v2|BI480386_P1 2866 532 82.6 globlastp 1331 LNU359brachypodium|09v1|DV474090_P1 2867 532 82.5 globlastp 1332 LNU359barley|10v1|BQ469878 2868 532 81.6 globlastp 1333 LNU359barley|10v2|BQ469878_T1 2869 532 81.45 glotblastn 1334 LNU360sugarcane|10v1|CA118302 2870 533 94.1 globlastp 1335 LNU360sorghum|09v1|SB10G006380 2871 533 90.9 globlastp 1336 LNU360foxtail_millet|10v2|SICRP000016_P1 2872 533 87.7 globlastp 1337 LNU360cynodon|10v1|ES302376_P1 2873 533 85.3 globlastp 1338 LNU360leymus|gb166|EG385922_P1 2874 533 84.2 globlastp 1339 LNU360oat|10v2|GR320403_P1 2875 533 83.8 globlastp 1340 LNU360oat|10v1|GR320403 2875 533 83.8 globlastp 1341 LNU360fescue|gb161|DT681344_P1 2876 533 82.9 globlastp 1342 LNU360brachypodium|09v1|GT772421_P1 2877 533 80.9 globlastp 1343 LNU361sorghum|09v1|SB04G025150 2878 534 92.2 globlastp 1344 LNU368wheat|gb164|BE400257 2879 541 92 globlastp 1345 LNU369brachypodium|09v1|GT787733_P1 2880 542 93.4 globlastp 1346 LNU369leymus|gb166|EG389109_P1 2881 542 90.1 globlastp 1347 LNU369rice|gb170|OS01G70100 2882 542 89.3 globlastp 1348 LNU369millet|10v1|EVO454PM068764_P1 2883 542 89.1 globlastp 1349 LNU369switchgrass|gb167|FE654078 2884 542 88.8 globlastp 1350 LNU369brachypodium|09v1|TMPLEG389109T1_P1 2885 542 88.7 globlastp 1351 LNU369maize|gb170|AI621555 2886 542 88.2 globlastp 1352 LNU369maize|10v1|AI621555_P1 2886 542 88.2 globlastp 1353 LNU369maize|10v1|AW308727_P1 2887 542 85.7 globlastp 1354 LNU369maize|gb170|AW308727 2887 542 85.7 globlastp 1355 LNU370potato|10v1|BG591992_P1 2888 543 99 globlastp 1356 LNU370solanum_phureja|09v1|SPHAI772811 2889 543 99 globlastp 1357 LNU370eggplant|10v1|FS023252_P1 2890 543 91.6 globlastp 1358 LNU370petunia|gb171|FN005093_T1 2891 543 81.65 glotblastn 1359 LNU371sorghum|09v1|SB03G041730 2892 544 88.12 glotblastn 1360 LNU373sorghum|09v1|SB10G026090 2893 546 85.9 globlastp 1361 LNU373maize|10v1|CD936590_P1 2894 546 85.5 globlastp 1362 LNU373maize|gb170|CD936590 2895 546 85.41 glotblastn 1363 LNU373brachypodium|09v1|DV483814_P1 2896 546 85.1 globlastp 1364 LNU373foxtail_millet|10v2|SICRP003144_T1 2897 546 84.65 glotblastn 1365 LNU373millet|10v1|EVO454PM064645_P1 2898 546 80.1 globlastp 1366 LNU375solanum_phureja|09v1|SPHBG125016 2899 548 95 globlastp 1367 LNU375potato|10v1|BF459523_P1 2900 548 94.4 globlastp 1368 LNU375pepper|gb171|AA840787_P1 2901 548 92.2 globlastp 1369 LNU375eggplant|10v1|FS016985_P1 2902 548 90 globlastp 1370 LNU375nicotiana_benthamiana|gb162| 2903 548 87 globlastp CK280835_P1 1371LNU376 sorghum|09v1|SB03G037440 2904 549 85.5 globlastp 1372 LNU378brachypodium|09v1|SRR031797S0001956_P1 2905 551 89 globlastp 1373 LNU379sugarcane|10v1|CA080471 2906 552 99 globlastp 1374 LNU379maize|10v1|CD440138_P1 2907 552 93.4 globlastp 1375 LNU379maize|gb170|CD440138 2907 552 93.4 globlastp 1376 LNU379millet|09v1|CD725143 2908 552 92.4 globlastp 1377 LNU379millet|10v1|CD725143_P1 2908 552 92.4 globlastp 1378 LNU379foxtail_millet|10v2|SICRP004364_P1 2909 552 92 globlastp 1379 LNU379cenchrus|gb166|EB659921_P1 2910 552 92 globlastp 1380 LNU379sugarcane|10v1|CA070722 2911 552 85.5 globlastp 1381 LNU379switchgrass|gb167|FE598481 2912 552 85.5 globlastp 1382 LNU379foxtail_millet|10v2|OXFXTSLX00041407D1T1_P1 2913 552 85.1 globlastp 1383LNU379 maize|10v1|AI712016_P1 2914 552 85.1 globlastp 1384 LNU379maize|gb170|AI712016 2914 552 85.1 globlastp 1385 LNU379millet|10v1|CD724799_P1 2915 552 84.8 globlastp 1386 LNU379millet|09v1|CD724799 2916 552 83.4 globlastp 1387 LNU379leymus|gb166|CD808758_P1 2917 552 83 globlastp 1388 LNU379wheat|10v2|BE404550_P1 2918 552 82.7 globlastp 1389 LNU379wheat|gb164|BE404550 2918 552 82.7 globlastp 1390 LNU379barley|10v1|BE413350 2919 552 82.4 globlastp 1391 LNU379barley|10v2|BE413350_P1 2919 552 82.4 globlastp 1392 LNU379brachypodium|09v1|DV474291_P1 2920 552 82 globlastp 1393 LNU379rice|gb170|OS08G18110 2921 552 82 globlastp 1394 LNU379oat|10v2|GO589632_P1 2922 552 80.6 globlastp 1395 LNU379oat|10v1|GO589632 2922 552 80.6 globlastp 1396 LNU381wheat|10v2|CA485868_P1 554 554 100 globlastp 1396 LNU381wheat|gb164|CA485868 2927 554 80.2 globlastp 1397 LNU381sugarcane|10v1|CA282730 2923 554 91.09 glotblastn 1398 LNU381maize|10v1|CO533611_P1 2924 554 87.1 globlastp 1399 LNU381maize|gb170|CO533611 2924 554 87.1 globlastp 1400 LNU381millet|10v1|PMSLX0058175D2_P1 2925 554 84.5 globlastp 1401 LNU381cenchrus|gb166|EB662230_P1 2926 554 84.2 globlastp 1402 LNU382arabidopsis_lyrata|09v1|JGIAL006288_P1 2928 555 90.5 globlastp 1403LNU383 solanum_phureja|09v1|SPHBG123484 2929 556 94.5 globlastp 1404LNU385 sorghum|09v1|SB03G014370 2930 558 88 globlastp 1405 LNU385brachypodium|09v1|DV471590_P1 2931 558 86.9 globlastp 1406 LNU385barley|10v2|AW983394_P1 2932 558 84.9 globlastp 1407 LNU385wheat|10v2|BE497298_P1 2933 558 84.6 globlastp 1408 LNU385wheat|gb164|BE497298 2934 558 82.8 globlastp 1409 LNU385barley|10v1|AW983394 2935 558 82.49 glotblastn 1410 LNU387maize|10v1|BG518113_P1 2936 560 93.7 globlastp 1411 LNU387maize|gb170|BG518113 2936 560 93.7 globlastp 1412 LNU387maize|10v1|AI600775_P1 2937 560 92.3 globlastp 1413 LNU387maize|gb170|AI600775 2937 560 92.3 globlastp 1414 LNU387rice|gb170|OS09G38420 2938 560 86.7 globlastp 1415 LNU387brachypodium|09v1|GT768177_P1 2939 560 82.6 globlastp 1416 LNU388brachypodium|09v1|GT785236_P1 2940 561 80.2 globlastp 1417 LNU390solanum_phureja|09v1|SPHBG125049 2941 562 88.7 globlastp 1418 LNU390solanum_phureja|09v1|SPHSRR015435S0020890 2942 562 80.2 globlastp 1419LNU391 wheat|10v2|BE499752_P1 2943 563 98.6 globlastp 1420 LNU391wheat|gb164|BE499752 2944 563 97.8 globlastp 1421 LNU391brachypodium|09v1|DV481377_P1 2945 563 93.5 globlastp 1422 LNU391fescue|gb161|DT686577_P1 2946 563 92.6 globlastp 1423 LNU391sorghum|09v1|SB01G032340 2947 563 84.1 globlastp 1424 LNU391rice|gb170|OS03G30790 2948 563 83.96 glotblastn 1425 LNU391maize|10v1|AW054498_P1 2949 563 83.3 globlastp 1426 LNU391maize|gb170|AW054498 2949 563 83.3 globlastp 1427 LNU391sugarcane|10v1|CA121549 2950 563 82.7 globlastp 1428 LNU391switchgrass|gb167|FL697122 2951 563 82.7 globlastp 1429 LNU391maize|10v1|AW288496_P1 2952 563 82.5 globlastp 1430 LNU391millet|10v1|EVO454PM001107_P1 2953 563 81.4 globlastp 1431 LNU392brachypodium|09v1|GT773267_P1 2954 564 85.1 globlastp 1431 LNU417_H4brachypodium|09v1|GT773267_P1 2954 677 80 globlastp 1431 LNU417brachypodium|09v1|GT773267_T1 2954 702 89.04 glotblastn 1432 LNU392maize|10v1|BM078460_P1 2955 564 83.8 globlastp 1432 LNU417_H4maize|10v1|BM078460_P1 2955 677 82.6 globlastp 1432 LNU417maize|10v1|BM078460_T1 2955 702 84.97 glotblastn 1433 LNU392sorghum|09v1|SB01G043030 2956 564 83 globlastp 1433 LNU417_H4sorghum|09v1|SB01G043030 2956 677 86.3 globlastp 1433 LNU417sorghum|09v1|SB01G043030 2956 702 87.12 glotblastn 1434 LNU392millet|10v1|EVO454PM040968_P1 2957 564 82.3 globlastp 1434 LNU417_H4millet|10v1|EVO454PM040968_P1 2957 677 82.8 globlastp 1434 LNU417millet|10v1|EVO454PM040968_T1 2957 702 84.43 glotblastn 1435 LNU392maize|gb170|BM078460 2958 564 80.3 globlastp 1435 LNU417_H4maize|gb170|BM078460 2958 677 80.97 glotblastn 1435 LNU417maize|gb170|BM078460 2958 702 84.97 glotblastn 1436 LNU393maize|10v1|EB166150_T1 2959 565 85.24 glotblastn 1437 LNU393maize|gb170|EB166150 2959 565 85.24 glotblastn 1438 LNU395maize|10v1|EU972999_P1 2960 566 85.6 globlastp 1439 LNU395maize|gb170|EU972999 2961 566 83.2 globlastp 1440 LNU396maize|10v1|AI670204_P1 2962 567 99.1 globlastp 1441 LNU396foxtail_millet|10v2|SICRP011369_P1 2963 567 97.2 globlastp 1442 LNU396sugarcane|10v1|CA075971 2964 567 96.3 globlastp 1443 LNU396switchgrass|gb167|DN151549 2965 567 96.3 globlastp 1444 LNU396millet|09v1|CD725448 2966 567 95.4 globlastp 1445 LNU396millet|10v1|CD725448_P1 2966 567 95.4 globlastp 1446 LNU396switchgrass|gb167|DN144669 2967 567 92.7 globlastp 1447 LNU396cenchrus|gb166|EB666773_P1 2968 567 92.6 globlastp 1448 LNU396fescue|gb161|DT674442_P1 2969 567 87.4 globlastp 1449 LNU396oat|10v2|CN815301_P1 2970 567 87.2 globlastp 1450 LNU396brachypodium|09v1|DV486678_P1 2971 567 86.5 globlastp 1451 LNU396rice|gb170|OS03G03770 2972 567 86.4 globlastp 1452 LNU396wheat|10v2|BG314370_P1 2973 567 84.7 globlastp 1453 LNU396barley|10v1|BE411304 2974 567 84.7 globlastp 1454 LNU396barley|10v2|BE411304_P1 2974 567 84.7 globlastp 1455 LNU396pseudoroegneria|gb167|FF358290 2975 567 84.7 globlastp 1456 LNU396wheat|gb164|BE404919 2973 567 84.7 globlastp 1457 LNU396wheat|gb164|BE405061 2973 567 84.7 globlastp 1458 LNU396wheat|gb164|BG909911 2976 567 83.8 globlastp 1459 LNU397switchgrass|gb167|DN142167 2977 568 87.6 globlastp 1460 LNU397foxtail_millet|10v2|SICRP040427_P1 2978 568 87.3 globlastp 1461 LNU401sugarcane|10v1|CA068996_P1 2979 570 93.7 globlastp 1462 LNU401maize|10v1|AI396373_T1 2980 570 84.44 glotblastn 1463 LNU401maize|10v1|DR792581_P1 2981 570 83.4 globlastp 1464 LNU402pseudoroegneria|gb167|FF345540 2982 571 98.7 globlastp 1465 LNU402brachypodium|09v1|SRR031798S0004329_P1 2983 571 83.5 globlastp 1466LNU403 maize|10v1|AI920392_T1 2984 572 87.96 glotblastn 1467 LNU403maize|10v1|BI417041_T1 2985 572 85.45 glotblastn 1468 LNU403maize|gb170|BI417041 2985 572 85.45 glotblastn 1469 LNU403switchgrass|gb167|DN145126 2986 572 82.41 glotblastn 1470 LNU403sugarcane|10v1|CA071352 2987 572 82.21 glotblastn 1471 LNU403maize|10v1|DR823873_T1 2988 572 82.04 glotblastn 1472 LNU403switchgrass|gb167|DN141486 2989 572 80.56 glotblastn 1473 LNU405potato|10v1|CK260581_T1 2990 573 86.18 glotblastn 1474 LNU408fescue|gb161|DT685544_T1 2991 575 91.07 glotblastn 1475 LNU408wheat|gb164|BE399717 2992 575 89.3 globlastp 1476 LNU408wheat|gb164|BQ170889 2992 575 89.3 globlastp 1477 LNU408wheat|10v2|BF485415_T1 2993 575 89.29 glotblastn 1478 LNU408wheat|10v2|BE399717_T1 2994 575 89.29 glotblastn 1479 LNU408wheat|10v2|BE414880_T1 2995 575 89.29 glotblastn 1480 LNU408wheat|gb164|BE414880 2996 575 89.29 glotblastn 1481 LNU408oat|10v2|SRR020741S0174434_P1 2997 575 87.5 globlastp 1482 LNU408oat|10v1|GO587069 2997 575 87.5 globlastp 1483 LNU408oat|10v2|GO587069_T1 2998 575 85.71 glotblastn 1484 LNU408brachypodium|09v1|DV470560_P1 2999 575 80.4 globlastp 1485 LNU408rye|gb164|BF145769 3000 575 80.36 glotblastn 1486 LNU408wheat|gb164|DR737280 3001 575 80.36 glotblastn 1487 LNU410brachypodium|09v1|DV473415_T1 3002 577 84.65 glotblastn 1488 LNU410oat|10v2|GR329792_T1 3003 577 82.61 glotblastn 1489 LNU411solanum_phureja|09v1|SPHBI207068 3004 578 96.1 globlastp 1490 LNU412cacao|10v1|CU619568_P1 3005 579 86.1 globlastp 1491 LNU413solanum_phureja|09v1|SPHBG126757 3006 580 97.7 globlastp 1492 LNU413pepper|gb171|BM063553_T1 3007 580 82.64 glotblastn 1493 LNU414brachypodium|09v1|SRR031795S0008555_P1 3008 581 88 globlastp 1494 LNU414barley|10v2|BE413415_P1 3009 581 87.8 globlastp 1495 LNU414rice|gb170|OS02G56310 3010 581 80.1 globlastp 1496 LNU415maize|10v1|AI391766_P1 3011 582 87.5 globlastp 1497 LNU415maize|gb170|AI391766 3011 582 87.5 globlastp 1498 LNU415foxtail_millet|10v2|SICRP012424_P1 3012 582 83.5 globlastp 1499 LNU419cacao|10v1|CA795077_P1 3013 585 83.5 globlastp 1500 LNU419chestnut|gb170|SRR006295S0002815_P1 3014 585 81.7 globlastp 1501 LNU419strawberry|11v1|DY666645_P1 3015 585 81.6 globlastp 1502 LNU419medicago|09v1|AI974351_P1 3016 585 81 globlastp 1503 LNU419pea|11v1|CD858805_P1 3017 585 80.4 globlastp 1504 LNU420wheat|10v2|CA484146_P1 586 586 100 globlastp 1505 LNU420wheat|gb164|CA484146 586 586 100 globlastp 1506 LNU420sugarcane|10v1|CA075634 3018 586 97.6 globlastp 1507 LNU420maize|10v1|AW562562_P1 3019 586 90.3 globlastp 1508 LNU420maize|gb170|AW562562 3019 586 90.3 globlastp 1509 LNU420switchgrass|gb167|FE618444 3020 586 86.6 globlastp 1510 LNU420millet|10v1|PMSLX0017470D1_P1 3021 586 86.1 globlastp 1511 LNU420foxtail_millet|10v2|SICRP015318_P1 3022 586 83.5 globlastp 1512 LNU422maize|10v1|AW067318_P1 3023 588 89.1 globlastp 1513 LNU422maize|gb170|AW067318 3023 588 89.1 globlastp 1514 LNU422millet|10v1|EVO454PM057489_P1 3024 588 87.4 globlastp 1515 LNU422switchgrass|gb167|FL823704 3025 588 87.4 globlastp 1516 LNU422barley|10v1|BF621668 3026 588 84.1 globlastp 1517 LNU422barley|10v2|BF621668_P1 3026 588 84.1 globlastp 1518 LNU422wheat|10v2|BE426240_P1 3027 588 84.1 globlastp 1519 LNU422wheat|gb164|BE426240 3027 588 84.1 globlastp 1520 LNU422leymus|gb166|EG392745_P1 3028 588 83.3 globlastp 1521 LNU422wheat|10v2|BE518320_P1 3029 588 83.2 globlastp 1522 LNU422wheat|gb164|BE518320 3030 588 82.8 globlastp 1523 LNU422brachypodium|09v1|DV473145_P1 3031 588 81.9 globlastp 1524 LNU422rice|gb170|OS08G04450 3032 588 80.8 globlastp 1525 LNU423maize|10v1|BE128849_P1 3033 589 87.6 globlastp 1526 LNU423maize|gb170|BE128849 3034 589 87.6 globlastp 1527 LNU423millet|10v1|EVO454PM020049_P1 3035 589 81.3 globlastp 1528 LNU424arabidopsis_lyrata|09v1|JGIAL019853_P1 3036 590 97.6 globlastp 1529LNU424 radish|gb164|EW734440 3037 590 95.3 globlastp 1530 LNU424radish|gb164|EW723032 3038 590 94.9 globlastp 1531 LNU424thellungiella|gb167|BY808370 3039 590 94.9 globlastp 1532 LNU424radish|gb164|EV524742 3040 590 94.5 globlastp 1533 LNU424b_juncea|10v2|E6ANDIZ01A8BJU_P1 3041 590 94.5 globlastp 1534 LNU424radish|gb164|EW733020 3042 590 94.1 globlastp 1535 LNU424b_oleracea|gb161|AM059842_P1 3043 590 93.7 globlastp 1536 LNU424b_rapa|gb162|CV545782_P1 3044 590 93.3 globlastp 1537 LNU424canola|10v1|CD817789_P1 3045 590 92.9 globlastp 1538 LNU424b_juncea|10v2|BJ1SLX00005485_P1 3046 590 89.7 globlastp 1539 LNU424cleome_gynandra|10v1|SRR015532S0005578_P1 3047 590 84.6 globlastp 1540LNU424 radish|gb164|EV539241 3048 590 82.6 globlastp 1541 LNU424b_juncea|gb164|EVGN00823111331395 3049 590 81 globlastp 1542 LNU424thellungiella|gb167|BY801711 3050 590 80.63 glotblastn 1543 LNU424senecio|gb170|DY660615 3051 590 80.24 glotblastn 1544 LNU425wheat|10v2|BE415800_P1 3052 591 97.9 globlastp 1545 LNU425wheat|gb164|CA701400 3052 591 97.9 globlastp 1546 LNU425brachypodium|09v1|SRR031797S0133764_P1 3053 591 89.7 globlastp 1547LNU425 wheat|gb164|BE415800 3054 591 85.7 globlastp 1548 LNU426cenchrus|gb166|EB657129_P1 3055 592 81.5 globlastp 1549 LNU426sugarcane|10v1|CA096527 3056 592 80.7 globlastp 1550 LNU426brachypodium|09v1|DV472433_P1 3057 592 80.5 globlastp 1551 LNU426switchgrass|gb167|DN147719 3058 592 80.3 globlastp 1552 LNU426foxtail_millet|10v2|SICRP009618_P1 3059 592 80.1 globlastp 1553 LNU426maize|10v1|CD996749_P1 3060 592 80 globlastp 1554 LNU426barley|10v1|BF065562 3061 592 80 glotblastn 1555 LNU426barley|10v2|BF065562_T1 3061 592 80 glotblastn 1556 LNU429solanum_phureja|09v1|SPHBG124215 3062 594 89.1 globlastp 1557 LNU429potato|10v1|BF460297_P1 3063 594 82.8 globlastp 1558 LNU429potato|10v1|BE922360_P1 3064 594 81.3 globlastp 1559 LNU430potato|10v1|BF154026_P1 3065 595 90.8 globlastp 1560 LNU430solanum_phureja|09v1|SPHBG134528 3066 595 89.7 globlastp 1561 LNU431maize|10v1|AW331095_T1 3067 596 82.95 glotblastn 1561 LNU431_H1maize|10v1|AW331095_T1 3067 716 87.11 glotblastn 1562 LNU432switchgrass|gb167|FL911295 3068 597 80.3 globlastp 1563 LNU433maize|10v1|EC858802_P1 3069 598 82.2 globlastp 1564 LNU433maize|gb170|EC858802 3069 598 82.2 globlastp 1565 LNU434maize|10v1|AI372108_P1 3070 599 83.7 globlastp 1566 LNU435wheat|gb164|BE400160 3071 600 96.6 globlastp 1567 LNU435wheat|10v2|BQ579132_P1 3072 600 96.6 globlastp 1568 LNU435wheat|gb164|BQ579132 3072 600 96.6 globlastp 1569 LNU435wheat|gb164|BI751574 3073 600 96.2 globlastp 1570 LNU435wheat|10v2|BE400160_P1 3073 600 96.2 globlastp 1571 LNU435brachypodium|09v1|DV473618_P1 3074 600 84.7 globlastp 1572 LNU436barley|10v1|BE413139 3075 601 96.3 globlastp 1573 LNU436barley|10v2|BE413139_P1 3075 601 96.3 globlastp 1574 LNU436wheat|10v2|BE418697_P1 3076 601 94.7 globlastp 1575 LNU436wheat|gb164|BE418697 3076 601 94.7 globlastp 1576 LNU437wheat|10v2|BM136523XX1_P1 3077 602 93.7 globlastp 1577 LNU437wheat|10v2|BM136523XX2_T1 3078 602 90.4 glotblastn 1578 LNU437brachypodium|09v1|DV485772_T1 3079 602 88.25 glotblastn 1578 LNU437_H2brachypodium|09v1|DV485772_P1 3079 679 82.2 globlastp 1579 LNU437maize|10v1|CD960306_T1 3080 602 86.26 glotblastn 1579 LNU437_H2maize|10v1|CD960306_P1 3080 679 82.4 globlastp 1580 LNU437wheat|gb164|BF293149 3081 602 86.2 globlastp 1581 LNU437maize|10v1|AI902127_T1 3082 602 85.43 glotblastn 1581 LNU437_H2maize|10v1|AI902127_P1 3082 679 81.9 globlastp 1582 LNU437wheat|10v2|BF293149_T1 3083 602 82.78 glotblastn 1583 LNU437sorghum|09v1|SB01G000220 3084 602 82.18 glotblastn 1584 LNU437maize|gb170|CD960306 3085 602 81.62 glotblastn 1585 LNU437maize|10v1|DW838041_T1 3086 602 81.52 glotblastn 1586 LNU437maize|gb170|AI902127 3087 602 80.79 glotblastn 1587 LNU437maize|gb170|DW838041 3088 602 80.79 glotblastn 1588 LNU437rice|gb170|OS07G33780 3089 602 80.17 glotblastn 1589 LNU438wheat|10v2|BE416560_P1 3090 603 98.3 globlastp 1590 LNU438wheat|gb164|BE416560 3090 603 98.3 globlastp 1591 LNU438brachypodium|09v1|GT783610_P1 3091 603 96.2 globlastp 1592 LNU438sorghum|09v1|SB10G022490 3092 603 93.6 globlastp 1593 LNU438rice|gb170|OS06G37160 3093 603 93.5 globlastp 1594 LNU438switchgrass|gb167|DN152570 3094 603 91.6 globlastp 1595 LNU438foxtail_millet|10v2|FXTRMSLX00002766D1_P1 3095 603 91.4 globlastp 1596LNU438 brachypodium|09v1|GFXEU730900X15_T1 3096 603 90.29 glotblastn1597 LNU438 millet|09v1|EVO454PM004612 3097 603 80.5 globlastp 1598LNU438 millet|10v1|EVO454PM004612_P1 3097 603 80.5 globlastp 1599 LNU438sugarcane|10v1|CA077281 3098 603 80.1 globlastp 1600 LNU439sugarcane|10v1|CA068568 3099 604 89.1 globlastp 1601 LNU441foxtail_millet|10v2|SICRP008836_T1 3100 605 88.85 glotblastn 1602 LNU441switchgrass|gb167|DN140960 3101 605 88.08 glotblastn 1603 LNU441wheat|gb164|CA502719 3102 605 87.5 globlastp 1604 LNU441maize|gb170|AA979820 3103 605 87.31 glotblastn 1605 LNU441maize|10v1|AA979820_T1 3104 605 86.59 glotblastn 1606 LNU441switchgrass|gb167|FE601692 3105 605 86.54 glotblastn 1607 LNU441millet|10v1|EVO454PM053619_T1 3106 605 83.85 glotblastn 1608 LNU441millet|09v1|EVO454PM009153 3107 605 83.85 glotblastn 1609 LNU441millet|10v1|EVO454PM009153_T1 3108 605 83.85 glotblastn 1610 LNU442potato|10v1|CV503625_T1 3109 606 89.2 glotblastn 1611 LNU444cotton|10v1|CO069493 3110 608 92.4 globlastp 1612 LNU444cotton|10v2|CO069742_P1 3111 608 92 globlastp 1613 LNU444castorbean|09v1|EE254681_P1 3112 608 84.6 globlastp 1614 LNU444chestnut|gb170|SRR006295S0008870_P1 3113 608 84.6 globlastp 1615 LNU444clementine|11v1|CB322234_P1 3114 608 83 globlastp 1616 LNU444orange|11v1|CB322234_P1 3114 608 83 globlastp 1617 LNU444citrus|gb166|CB322234_P1 3114 608 83 globlastp 1618 LNU444tamarix|gb166|CD151484 3115 608 82.5 globlastp 1619 LNU444spurge|gb161|DR066805 3116 608 82.2 globlastp 1620 LNU444cleome_gynandra|10v1|SRR015532S0000170_P1 3117 608 81.7 globlastp 1621LNU444 cleome_spinosa|10v1|GR931499_P1 3118 608 81.7 globlastp 1622LNU444 beech|gb170|AM062846_T1 3119 608 81.03 glotblastn 1623 LNU444cucumber|09v1|AA660032_P1 3120 608 80.9 globlastp 1624 LNU444cowpea|gb166|AF139468_P1 3121 608 80.6 globlastp 1625 LNU444b_juncea|gb164|EVGN00773211733256 3122 608 80.5 globlastp 1626 LNU444eucalyptus|11v1|ES591008_P1 3123 608 80.3 globlastp 1627 LNU444grape|gb160|BM436503_P1 3124 608 80.1 globlastp 1628 LNU444melon|10v1|AM716315_P1 3125 608 80.1 globlastp 1629 LNU444melon|gb165|AM716315 3125 608 80.1 globlastp 1630 LNU445soybean|11v1|GLYMA14G08480_P1 3126 609 93.2 globlastp 1631 LNU445soybean|gb168|FK293250 3127 609 89.4 globlastp 1632 LNU446soybean|11v1|GLYMA06G05570_T1 3128 610 96.52 glotblastn 1633 LNU446pigeonpea|10v1|SRR054580S0024764_P1 3129 610 93 globlastp 1634 LNU446lotus|09v1|LLGO005719_P1 3130 610 90.7 globlastp 1635 LNU446medicago|09v1|AW328864_P1 3131 610 88.2 globlastp 1636 LNU446peanut|10v1|GO326813_P1 3132 610 86.9 globlastp 1637 LNU446soybean|gb168|BI968704 3133 610 86.78 glotblastn 1638 LNU446soybean|11v1|GLYMA14G11090_P1 3134 610 84.8 globlastp 1639 LNU446soybean|11v1|GLYMA17G34500_P1 3135 610 84.6 globlastp 1640 LNU446peanut|10v1|CX127972_P1 3136 610 83.4 globlastp 1641 LNU446prunus|10v1|CN940235_P1 3137 610 80.2 globlastp 1641 LNU464prunus|10v1|CN940235_P1 3137 627 81.6 globlastp 1642 LNU448leymus|gb166|EG393138_P1 3138 612 95.3 globlastp 1643 LNU448wheat|gb164|BE404484 3139 612 93.9 globlastp 1644 LNU448pseudoroegneria|gb167|FF342552 3140 612 93.5 globlastp 1645 LNU448wheat|10v2|BE404484_P1 3141 612 93.5 globlastp 1646 LNU448wheat|10v2|BE490784_P1 3142 612 91.1 globlastp 1647 LNU448wheat|gb164|BE405353 3143 612 85.6 globlastp 1648 LNU448oat|10v2|GR366131_P1 3144 612 85.3 globlastp 1649 LNU448oat|10v1|GR366131 3145 612 84.8 globlastp 1650 LNU448wheat|gb164|BE490784 3146 612 83.1 globlastp 1651 LNU448fescue|gb161|CK801098_T1 3147 612 81.49 glotblastn 1652 LNU449cotton|10v1|AI727881 3148 613 89.5 globlastp 1653 LNU449cotton|10v2|BE053391_P1 3149 613 89.5 globlastp 1654 LNU449cotton|10v2|BE054720_P1 3150 613 89.1 globlastp 1655 LNU449cotton|10v2|ES838489_P1 3150 613 89.1 globlastp 1656 LNU449cotton|10v1|BQ410208 3150 613 89.1 globlastp 1657 LNU449cacao|10v1|CA794300_P1 3151 613 88 globlastp 1658 LNU449cacao|gb167|CA794300 3152 613 87.7 globlastp 1659 LNU449chestnut|gb170|SRR006295S0000582_P1 3153 613 87.7 globlastp 1660 LNU449oak|10v1|FP027403_P1 3154 613 87.3 globlastp 1661 LNU449clementine|11v1|CB292027_P1 3155 613 87 globlastp 1662 LNU449orange|11v1|CB292027_P1 3156 613 87 globlastp 1663 LNU449citrus|gb166|CB292027_P1 3155 613 87 globlastp 1664 LNU449momordica|10v1|SRR071315S0012320_P1 3157 613 86.6 globlastp 1665 LNU449cucumber|09v1|AM716760_P1 3158 613 86.2 globlastp 1666 LNU449papaya|gb165|AM904488_P1 3159 613 85.9 globlastp 1667 LNU449oak|10v1|SRR006309S0002232_T1 3160 613 85.51 glotblastn 1668 LNU449melon|10v1|AM716760_P1 3161 613 85.5 globlastp 1669 LNU449tea|10v1|GH159051 3162 613 84.8 globlastp 1670 LNU449melon|gb165|AM716760 3163 613 84.42 glotblastn 1671 LNU449eucalyptus|11v1|CD668460_P1 3164 613 84.4 globlastp 1672 LNU449prunus|10v1|AF139498_P1 3165 613 84.4 globlastp 1673 LNU449apple|gb171|CN495313_P1 3166 613 84.4 globlastp 1674 LNU449poplar|10v1|BI131224_P1 3167 613 84.4 globlastp 1675 LNU449eschscholzia|10v1|CD476462_P1 3168 613 84.1 globlastp 1676 LNU449prunus|gb167|AF139498 3169 613 84.1 globlastp 1677 LNU449castorbean|09v1|EE257410_P1 3170 613 83.7 globlastp 1678 LNU449coffea|10v1|DV665955_P1 3171 613 83.7 globlastp 1679 LNU449poplar|10v1|BU820883_P1 3172 613 83.7 globlastp 1680 LNU449tobacco|gb162|DW002390 3173 613 83.7 globlastp 1681 LNU449apple|gb171|CN865353_P1 3174 613 83.3 globlastp 1682 LNU449liriodendron|gb166|DT601421_P1 3175 613 83.3 globlastp 1683 LNU449tobacco|gb162|EB425519 3176 613 83 globlastp 1684 LNU449cassava|09v1|BM259855_P1 3177 613 82.6 globlastp 1685 LNU449grape|gb160|CB343473_P1 3178 613 82.6 globlastp 1686 LNU449spurge|gb161|BI962025 3179 613 82.6 globlastp 1687 LNU449petunia|gb171|FN001394_P1 3180 613 82.2 globlastp 1688 LNU449potato|10v1|BE920222_P1 3181 613 82.2 globlastp 1689 LNU449solanum_phureja|09v1|SPHBG127776 3181 613 82.2 globlastp 1690 LNU449sunflower|10v1|CD847752_P1 3182 613 82.2 globlastp 1691 LNU449sunflower|gb162|CD847752 3182 613 82.2 globlastp 1692 LNU449tragopogon|10v1|SRR020205S0015413 3183 613 82.2 globlastp 1693 LNU449liriodendron|gb166|CO996218_P1 3184 613 81.9 globlastp 1694 LNU449petunia|gb171|CV292815_P1 3185 613 81.9 globlastp 1695 LNU449tomato|09v1|BG127776 3186 613 81.9 globlastp 1696 LNU449lettuce|10v1|DW076391_T1 3187 613 81.88 glotblastn 1697 LNU449centaurea|gb166|EH724535_P1 3188 613 81.5 globlastp 1698 LNU449cichorium|gb171|DT213172_P1 3189 613 81.5 globlastp 1699 LNU449coffea|10v1|DV664677_P1 3190 613 81.5 globlastp 1700 LNU449eggplant|10v1|FS002597_P1 3191 613 81.5 globlastp 1701 LNU449lotus|09v1|LLAI967358_P1 3192 613 81.5 globlastp 1702 LNU449parthenium|10v1|GW781311_P1 3193 613 81.2 globlastp 1703 LNU449pigeonpea|10v1|SRR054580S0001717_P1 3194 613 81.2 globlastp 1704 LNU449pepper|gb171|BM068079_P1 3195 613 81.2 globlastp 1705 LNU449oak|10v1|FP028757_P1 3196 613 80.8 globlastp 1706 LNU449peanut|10v1|ES707534_P1 3197 613 80.8 globlastp 1707 LNU449avocado|10v1|CK751924_T1 3198 613 80.8 glotblastn 1708 LNU449ipomoea_nil|10v1|BJ559339_P1 3199 613 80.8 globlastp 1709 LNU449lettuce|10v1|DW054122_P1 3200 613 80.8 globlastp 1710 LNU449nasturtium|10v1|SRR032558S0042354_T1 3201 613 80.8 glotblastn 1711LNU449 strawberry|11v1|CO816865_P1 3202 613 80.8 globlastp 1712 LNU449strawberry|gb164|CO816865 3202 613 80.8 globlastp 1713 LNU449senecio|gb170|DY658995 3203 613 80.4 globlastp 1714 LNU449sunflower|10v1|DY945543_P1 3204 613 80.4 globlastp 1715 LNU449sunflower|gb162|DY945543 3204 613 80.4 globlastp 1716 LNU451solanum_phureja|09v1|SPHBG124246 3205 615 96.1 globlastp 1717 LNU451pepper|gb171|CA523377_P1 3206 615 91.4 globlastp 1718 LNU451tobacco|gb162|DV160269 3207 615 86.4 globlastp 1719 LNU451monkeyflower|10v1|DV209953_P1 3208 615 80 globlastp 1720 LNU453maize|10v1|AW400263_P1 3209 616 93.1 globlastp 1721 LNU453maize|10v1|DW740014_P1 3210 616 81.5 globlastp 1722 LNU453maize|gb170|DW740014 3210 616 81.5 globlastp 1723 LNU453switchgrass|gb167|FL712148 3211 616 81.36 glotblastn 1724 LNU455potato|10v1|BG888608_P1 3212 618 96.7 globlastp 1725 LNU455solanum_phureja|09v1|SPHBG626661 3212 618 96.7 globlastp 1726 LNU455tobacco|gb162|EB426860 3213 618 90 globlastp 1727 LNU455nicotiana_benthamiana|gb162|EX534033_P1 3214 618 88.6 globlastp 1728LNU455 petunia|gb171|CV296478_P1 3215 618 87.6 globlastp 1729 LNU456wheat|gb164|BE216917 3216 619 95.4 globlastp 1730 LNU456wheat|10v2|BE216917_P1 3217 619 95.4 globlastp 1731 LNU456pseudoroegneria|gb167|FF367249 3218 619 94.9 globlastp 1732 LNU456wheat|gb164|BF293470 3219 619 94.9 globlastp 1733 LNU456wheat|gb164|BF474913 3220 619 94.5 globlastp 1734 LNU456brachypodium|09v1|GT778786_P1 3221 619 85 globlastp 1735 LNU457tomato|09v1|BQ512773_T1 3222 620 89.69 glotblastn 1736 LNU460sugarcane|10v1|CA079961 3223 623 94.2 globlastp 1737 LNU460sorghum|09v1|SB01G001140 3224 623 93.7 globlastp 1738 LNU460switchgrass|gb167|FE610157 3225 623 89.3 globlastp 1739 LNU460wheat|10v2|BG606900_P1 3226 623 86.5 globlastp 1740 LNU460rice|gb170|OS03G63330 3227 623 85.1 globlastp 1741 LNU460brachypodium|09v1|DV485015_P1 3228 623 84.9 globlastp 1742 LNU460wheat|gb164|BE429280 3229 623 83 globlastp 1743 LNU460fescue|gb161|DT685320_P1 3230 623 82.4 globlastp 1744 LNU460oat|10v2|CN817353_T1 3231 623 82.39 glotblastn 1745 LNU460oat|10v1|CN817353 3232 623 82.39 glotblastn 1746 LNU460wheat|10v2|BE429280_T1 3233 623 80 glotblastn 1747 LNU462solanum_phureja|09v1|SPHAI896771 3234 625 93.1 globlastp 1748 LNU462tomato|09v1|BG630881 3235 625 80.7 globlastp 1749 LNU462solanum_phureja|09v1|SPHBG630881 3236 625 80.5 globlastp 1750 LNU464oak|10v1|FP067463_P1 3237 627 83.3 globlastp 1751 LNU464monkeyflower|10v1|GR019400_P1 3238 627 80.1 globlastp 1752 LNU466wheat|10v2|BE443236_P1 3239 629 97 globlastp 1753 LNU466wheat|gb164|BE443236 3240 629 91.6 globlastp 1754 LNU466brachypodium|09v1|GT778423_P1 3241 629 91.1 globlastp 1755 LNU466wheat|10v2|CA497187_P1 3242 629 82.9 globlastp 1756 LNU466rice|gb170|OS12G39160 3243 629 82.8 globlastp 1757 LNU466sorghum|09v1|SB01G014910 3244 629 81.7 globlastp 1758 LNU466rice|gb170|OS03G40930 3245 629 80.8 globlastp 1759 LNU466brachypodium|09v1|GT822143_P1 3246 629 80.6 globlastp 1760 LNU467wheat|10v2|BE418022XX1_P1 3247 630 96 globlastp 1761 LNU467wheat|gb164|BE418022 3248 630 95.8 globlastp 1762 LNU467brachypodium|09v1|GT768192_P1 3249 630 85.9 globlastp 1763 LNU467rice|gb170|OS01G33800 3250 630 80.3 globlastp 1764 LNU468potato|10v1|BI405533_P1 3251 631 97.1 globlastp 1765 LNU468solanum_phureja|09v1|SPHAI637280 3251 631 97.1 globlastp 1766 LNU469sorghum|09v1|SB06G024340 3252 632 90.5 globlastp 1767 LNU472switchgrass|gb167|FL834062 3253 635 85.2 globlastp 1768 LNU472sorghum|09v1|SB03G023773 3254 635 83.6 globlastp 1769 LNU472millet|10v1|PMSLX0282794D1_P1 3255 635 83 globlastp 1770 LNU473maize|10v1|CD956410_P1 3256 636 92.3 globlastp 1771 LNU473maize|gb170|LLCD956410 3256 636 92.3 globlastp 1772 LNU473foxtail_millet|10v2|SICRP007698_T1 3257 636 87.03 glotblastn 1773 LNU477sugarcane|10v1|BQ532957 3258 639 99.4 globlastp 1774 LNU477foxtail_millet|10v2|SICRP038587_P1 3259 639 98.4 globlastp 1775 LNU477switchgrass|gb167|FE613133 3260 639 98 globlastp 1776 LNU477maize|10v1|AI944016_P1 3261 639 97.4 globlastp 1777 LNU477maize|gb170|AI944016 3261 639 97.4 globlastp 1778 LNU477millet|10v1|EVO454PM006681_P1 3262 639 96 globlastp 1779 LNU477wheat|10v2|BE604866_P1 3263 639 95.4 globlastp 1780 LNU477wheat|gb164|BE403167 3263 639 95.4 globlastp 1781 LNU477barley|10v1|BE438172 3264 639 95 globlastp 1782 LNU477barley|10v2|BE438172_P1 3264 639 95 globlastp 1783 LNU477brachypodium|09v1|GT769985_P1 3265 639 95 globlastp 1784 LNU477rice|gb170|OS03G21950 3266 639 95 globlastp 1785 LNU477aristolochia|10v1|FD755001_P1 3267 639 87.2 globlastp 1786 LNU477aquilegia|10v2|DR929807_P1 3268 639 86.1 globlastp 1787 LNU477eucalyptus|11v1|CU396262_P1 3269 639 85.8 globlastp 1788 LNU477prunus|10v1|BU040396_P1 3270 639 85.8 globlastp 1789 LNU477cotton|10v1|AI725667 3271 639 85.8 globlastp 1790 LNU477aquilegia|10v2|JGIAC017994_P1 3272 639 85.7 globlastp 1791 LNU477clementine|11v1|CB292429_P1 3273 639 85.6 globlastp 1791 LNU477orange|11v1|CB292429_P1 3273 639 85.6 globlastp 1792 LNU477cotton|10v2|SRR032367S0638081_P1 3274 639 85.6 globlastp 1793 LNU477oak|10v1|FP028605_P1 3275 639 85.6 globlastp 1794 LNU477cotton|10v2|SRR032367S0193380_P1 3276 639 85.6 globlastp 1795 LNU477citrus|gb166|CB292429_P1 3273 639 85.6 globlastp 1796 LNU477cotton|10v2|SRR032367S0065913_T1 3277 639 85.03 glotblastn 1797 LNU477melon|10v1|DV631444_P1 3278 639 85 globlastp 1798 LNU477apple|gb171|CN489928_P1 3279 639 84.8 globlastp 1799 LNU477poplar|10v1|BI069889_P1 3280 639 84.8 globlastp 1800 LNU477cacao|10v1|CU584416_P1 3281 639 84.6 globlastp 1801 LNU477bean|gb167|CA900306_P1 3282 639 84.6 globlastp 1802 LNU477cowpea|gb166|FF390148_P1 3283 639 84.6 globlastp 1803 LNU477monkeyflower|10v1|GR015985_P1 3284 639 84.4 globlastp 1804 LNU477triphysaria|10v1|DR173958 3285 639 84.3 globlastp 1805 LNU477lotus|09v1|CB826761_P1 3286 639 84.2 globlastp 1806 LNU477sunflower|10v1|DY921185_P1 3287 639 84.2 globlastp 1807 LNU477strawberry|11v1|CO381683_P1 3288 639 84 globlastp 1808 LNU477orobanche|10v1|SRR023189S0000238_P1 3289 639 84 globlastp 1809 LNU477zostera|10v1|SRR057351S0001126_P1 3290 639 83.9 globlastp 1810 LNU477arabidopsis_lyrata|09v1|JGIAL016174_P1 3291 639 83.8 globlastp 1811LNU477 soybean|11v1|GLYMA10G02040_P1 3292 639 83.8 globlastp 1812 LNU477soybean|gb168|AL374333 3293 639 83.8 globlastp 1813 LNU477sunflower|gb162|DY921185 3294 639 83.8 globlastp 1814 LNU477soybean|11v1|GLYMA02G01920_P1 3295 639 83.8 globlastp 1815 LNU477dandelion|10v1|DY805862_T1 3296 639 83.73 glotblastn 1816 LNU477arabidopsis|10v1|AT2G47510_P1 3297 639 83.6 globlastp 1817 LNU477arabidopsis|gb165|AT2G47510 3297 639 83.6 globlastp 1818 LNU477radish|gb164|EV529214 3298 639 83.4 globlastp 1819 LNU477soybean|gb168|CA900306 3299 639 83.4 globlastp 1820 LNU477centaurea|gb166|EH721673_T1 3300 639 83.13 glotblastn 1821 LNU477lettuce|10v1|DW046954_T1 3301 639 83.13 glotblastn 1822 LNU477podocarpus|10v1|SRR065014S0003290_T1 3302 639 83.03 glotblastn 1823LNU477 sunflower|10v1|DY911213_P1 3303 639 83 globlastp 1824 LNU477pepper|gb171|BM064125_P1 3304 639 83 globlastp 1825 LNU477pigeonpea|10v1|SRR054580S0047814_P1 3305 639 82.8 globlastp 1826 LNU477potato|10v1|BG591774_P1 3306 639 82.6 globlastp 1827 LNU477solanum_phureja|09v1|SPHAI895415 3306 639 82.6 globlastp 1828 LNU477tomato|09v1|AW648564 3307 639 82.4 globlastp 1829 LNU477tomato|09v1|BG642408 3308 639 82.2 globlastp 1830 LNU477dandelion|gb161|DY805862 3309 639 82 globlastp 1831 LNU477prunus|gb167|BU040396 3310 639 81.93 glotblastn 1832 LNU477cassava|09v1|CK649367_P1 3311 639 81.9 globlastp 1833 LNU477medicago|09v1|AL374333_P1 3312 639 81.8 globlastp 1834 LNU477spruce|gb162|CO222288 3313 639 81.8 globlastp 1835 LNU477antirrhinum|gb166|AJ558600_T1 3314 639 81.73 glotblastn 1836 LNU477artemisia|10v1|EY076361_P1 3315 639 81.7 globlastp 1837 LNU477b_rapa|gb162|CV434106_P1 3316 639 81.7 globlastp 1838 LNU477pine|10v2|BF049732_P1 3317 639 81.6 globlastp 1839 LNU477arabidopsis|10v1|AT5G50950_P1 3318 639 81.6 globlastp 1840 LNU477arabidopsis|gb165|AT5G50950 3318 639 81.6 globlastp 1841 LNU477pine|10v1|BE996818 3317 639 81.6 globlastp 1842 LNU477pseudotsuga|10v1|SRR065119S0016339_P1 3319 639 81.5 globlastp 1843LNU477 castorbean|09v1|EV520386_P1 3320 639 81.4 globlastp 1844 LNU477arabidopsis_lyrata|09v1|JGIAL029515_P1 3321 639 81 globlastp 1845 LNU477canola|10v1|CD835523_P1 3322 639 80.8 globlastp 1846 LNU477cucumber|09v1|ES597099_P1 3323 639 80.2 globlastp 1847 LNU479sugarcane|10v1|CA099284 3324 640 91.9 globlastp 1848 LNU479maize|10v1|AI615138_P1 3325 640 89.5 globlastp 1849 LNU479maize|gb170|AI615138 3325 640 89.5 globlastp 1850 LNU479maize|10v1|AI740031_P1 3326 640 88.5 globlastp 1851 LNU479maize|gb170|AI740031 3326 640 88.5 globlastp 1852 LNU479foxtail_millet|10v2|SICRP003741_P1 3327 640 80.7 globlastp 1853 LNU480maize|10v1|AW018101_P1 3328 641 96.7 globlastp 1854 LNU480maize|gb170|AW018101 3328 641 96.7 globlastp 1855 LNU480rice|gb170|OS03G60460 3329 641 92.6 globlastp 1856 LNU480brachypodium|09v1|GT768427_P1 3330 641 90.2 globlastp 1857 LNU480millet|10v1|CD725477_P1 3331 641 80.4 globlastp 1858 LNU481maize|10v1|BM348553_P1 3332 642 86.5 globlastp 1859 LNU481maize|gb170|BM348553 3332 642 86.5 globlastp 1860 LNU481switchgrass|gb167|FE624299 3333 642 81.8 globlastp 1861 LNU481foxtail_millet|10v2|FXTRMSLX00502435D1_P1 3334 642 81.4 globlastp 1862LNU481 switchgrass|gb167|DN141959 3335 642 81.3 globlastp 1863 LNU486rice|gb170|OS08G04540 3336 646 87.4 globlastp 1864 LNU486millet|10v1|EVO454PM108169_P1 3337 646 85.9 globlastp 1865 LNU486maize|10v1|DR797096_P1 3338 646 84.5 globlastp 1866 LNU486maize|gb170|DR797096 3338 646 84.5 globlastp 1867 LNU486millet|10v1|PMSLX0008075D1_P1 3339 646 84.3 globlastp 1868 LNU486sorghum|09v1|SB07G003020 3340 646 84.3 globlastp 1869 LNU486brachypodium|09v1|SRR031795S0042968_P1 3341 646 84.2 globlastp 1870LNU486 sorghum|09v1|SB07G003040 3342 646 83.7 globlastp 1871 LNU486maize|10v1|DT650994_P1 3343 646 83.6 globlastp 1872 LNU486maize|gb170|SRR014549S0325734 3344 646 83 globlastp 1873 LNU486maize|10v1|SRR014549S0325735_P1 3345 646 82.7 globlastp 1874 LNU486brachypodium|09v1|GT763377_P1 3346 646 81.6 globlastp 1875 LNU486brachypodium|09v1|SRR031797S0000753_P1 3347 646 81.6 globlastp 1876LNU486 brachypodium|09v1|DV484841_P1 3348 646 81.1 globlastp 1877 LNU486maize|10v1|GRMZM2G441632T01_P1 3349 646 81.1 globlastp 1878 LNU486barley|10v1|BI947839 3350 646 80.9 globlastp 1879 LNU486brachypodium|09v1|DV473894_P1 3351 646 80.9 globlastp 1880 LNU486maize|gb170|CRPZM2N004754 3352 646 80.7 globlastp 1881 LNU486wheat|10v2|BM135610_P1 3353 646 80.5 globlastp 1882 LNU486barley|10v2|BI951142_P1 3354 646 80.5 globlastp 1883 LNU486brachypodium|09v1|BRADI2G02370_P1 3355 646 80 globlastp 1884 LNU489solanum_phureja|09v1|SPHBG132312 3356 647 97.5 globlastp 1885 LNU490poplar|10v1|BU808912_T1 3357 648 86.43 glotblastn 1886 LNU490castorbean|09v1|XM002517212_P1 3358 648 84.3 globlastp 1887 LNU490cassava|09v1|DB921878_P1 3359 648 80.7 globlastp 1888 LNU492switchgrass|gb167|DN145159 3360 650 89.76 glotblastn 1889 LNU492millet|10v1|EVO454PM001690_P1 3361 650 89.4 globlastp 1890 LNU492sorghum|09v1|SB02G042100 3362 650 89.12 glotblastn 1891 LNU492brachypodium|09v1|GT758722_P1 3363 650 87.1 globlastp 1892 LNU492maize|gb170|AW067292 3364 650 85.74 glotblastn 1893 LNU492barley|10v1|AV834942 3365 650 85.1 globlastp 1894 LNU492barley|10v2|AV834942_P1 3365 650 85.1 globlastp 1895 LNU492maize|10v1|AW067292_T1 3366 650 85.01 glotblastn 1896 LNU493switchgrass|gb167|DN144413 3367 651 86.4 globlastp 1897 LNU493oat|10v2|GR318288_P1 3368 651 86.1 globlastp 1898 LNU493oat|10v1|GR318288 3368 651 86.1 globlastp 1899 LNU493wheat|10v2|BQ166641_P1 3369 651 85.7 globlastp 1900 LNU493wheat|10v2|BF478823_P1 3370 651 85.4 globlastp 1901 LNU493brachypodium|09v1|DV469482_P1 3371 651 85.2 globlastp 1902 LNU493wheat|gb164|BF478823 3372 651 85.2 globlastp 1903 LNU493sugarcane|10v1|BU102536 3373 651 84.94 glotblastn 1904 LNU493millet|10v1|EB411086_T1 3374 651 84.63 glotblastn 1905 LNU493sorghum|09v1|SB10G020910 3375 651 84.48 glotblastn 1906 LNU493wheat|gb164|BI751896 3376 651 84.4 globlastp 1907 LNU493wheat|10v2|BI751896_T1 3377 651 84.08 glotblastn 1908 LNU493barley|10v1|AV833693 3378 651 83.4 globlastp 1909 LNU493barley|10v2|AV833693_P1 3378 651 83.4 globlastp 1910 LNU493maize|gb170|AI902081 3379 651 82.7 globlastp 1911 LNU493maize|10v1|AI902081_P1 3380 651 82.5 globlastp 1912 LNU493pseudoroegneria|gb167|FF349115 3381 651 81.8 glotblastn 1913 LNU493leymus|gb166|EG376779_P1 3382 651 81.1 globlastp 1914 LNU494sorghum|09v1|SB02G011210 3383 652 86.8 globlastp 1915 LNU494maize|gb170|BI478378 3384 652 80.32 glotblastn 1916 LNU496barley|10v1|CD054173 3385 654 87.3 globlastp 1917 LNU496barley|10v2|CD054173_P1 3385 654 87.3 globlastp 1918 LNU496brachypodium|09v1|DV473745_P1 3386 654 85.3 globlastp 1919 LNU496rice|gb170|OS06G46330 3387 654 80.6 globlastp 1919 LNU520rice|gb170|OS06G46330 3387 675 80.9 globlastp 1920 LNU497wheat|10v2|BE405826XX1_P1 3388 655 95.1 globlastp 1921 LNU497leymus|gb166|CD808855_P1 3389 655 93.7 globlastp 1922 LNU497barley|10v1|BE437367 3390 655 92.3 globlastp 1923 LNU497barley|10v2|BE437367_T1 3391 655 92.08 glotblastn 1924 LNU497barley|10v1|BI951306 3392 655 91.8 globlastp 1925 LNU497barley|10v2|BI951306_P1 3392 655 91.8 globlastp 1926 LNU497wheat|gb164|BE400632 3393 655 91.8 glotblastn 1927 LNU497wheat|10v2|BE400632_P1 3394 655 91.3 globlastp 1928 LNU497wheat|10v2|BE400438_P1 3395 655 90.2 globlastp 1929 LNU497wheat|gb164|BE399352 3396 655 88.7 globlastp 1930 LNU497wheat|gb164|BE405826 3397 655 87.9 globlastp 1931 LNU497wheat|gb164|BE400438 3398 655 85.9 globlastp 1932 LNU497oat|10v2|CN815673_P1 3399 655 84.3 globlastp 1933 LNU497brachypodium|09v1|DV469731_P1 3400 655 83.9 globlastp 1934 LNU497wheat|gb164|CA607613 3401 655 82.8 globlastp 1935 LNU498sugarcane|10v1|CA120232 3402 656 94.9 globlastp 1936 LNU498switchgrass|gb167|GD007288 3403 656 90.5 globlastp 1937 LNU498brachypodium|09v1|GT763740_P1 3404 656 82.7 globlastp 1937 LNU499brachypodium|09v1|GT763740_P1 3404 657 85.6 globlastp 1938 LNU498rice|gb170|OS07G05365 3405 656 82 globlastp 1938 LNU499rice|gb170|OS07G05365 3405 657 80.6 globlastp 1939 LNU498wheat|10v2|BE591194_P1 3406 656 80.6 globlastp 1939 LNU499wheat|10v2|BE591194_P1 3406 657 94.2 globlastp 1940 LNU499fescue|gb161|CK801688_P1 3407 657 88.5 globlastp 1941 LNU500potato|10v1|BF153480_P1 3408 658 97.9 globlastp 1942 LNU500solanum_phureja|09v1|SPHBG127476 3408 658 97.9 globlastp 1943 LNU500tobacco|gb162|EB677931 3409 658 92.9 globlastp 1944 LNU500melon|10v1|AM720613_P1 3410 658 83.6 globlastp 1945 LNU500triphysaria|10v1|DR171672 3411 658 82.8 globlastp 1946 LNU500cucumber|09v1|CK760287_P1 3412 658 82.7 globlastp 1947 LNU500melon|gb165|AM720613 3413 658 82.4 globlastp 1948 LNU500oak|10v1|FP033820_P1 3414 658 82.2 globlastp 1949 LNU500cotton|10v1|BQ407081 3415 658 82.1 globlastp 1950 LNU500monkeyflower|10v1|CV517084_P1 3416 658 82.1 globlastp 1951 LNU500citrus|gb166|CX073916_P1 3417 658 82 globlastp 1952 LNU500arabidopsis_lyrata|09v1|JGIAL009912_P1 3418 658 81.7 globlastp 1953LNU500 bean|gb167|CA900254_P1 3419 658 81.7 globlastp 1954 LNU500b_rapa|gb162|CX268091_P1 3420 658 81.4 globlastp 1955 LNU500medicago|09v1|AW689365_P1 3421 658 81.2 globlastp 1956 LNU500poplar|10v1|BI072351_P1 3422 658 81.2 globlastp 1957 LNU500canola|10v1|CD825188_P1 3423 658 81 globlastp 1958 LNU500soybean|11v1|GLYMA19G36580_P1 3424 658 81 globlastp 1959 LNU500soybean|gb168|BU545791 3424 658 81 globlastp 1960 LNU500cotton|10v1|BG440074 3425 658 80.9 globlastp 1961 LNU500cotton|10v2|BG440074_P1 3426 658 80.9 globlastp 1962 LNU500arabidopsis|10v1|AT3G14390_P1 3427 658 80.8 globlastp 1963 LNU500arabidopsis|gb165|AT3G14390 3427 658 80.8 globlastp 1964 LNU500nasturtium|10v1|SRR032558S0026061_P1 3428 658 80.7 globlastp 1965 LNU500soybean|11v1|GLYMA03G33830_P1 3429 658 80.6 globlastp 1966 LNU500soybean|gb168|AW689365 3429 658 80.6 globlastp 1967 LNU500castorbean|09v1|EE260114_P1 3430 658 80.4 globlastp 1968 LNU500eucalyptus|11v1|SRR001659X10631_P1 3431 658 80.2 globlastp 1969 LNU500prunus|10v1|BU041335_P1 3432 658 80.2 globlastp 1970 LNU500poplar|10v1|CF233615_P1 3433 658 80.2 globlastp 1971 LNU500cassava|09v1|CK645527_P1 3434 658 80.2 globlastp 1972 LNU500canola|10v1|CN830386_P1 3435 658 80.1 globlastp 1973 LNU500sunflower|gb162|CX947049 3436 658 80.1 globlastp 1974 LNU501sugarcane|10v1|BQ804027 3437 659 99.1 globlastp 1975 LNU501maize|10v1|BM382690_P1 3438 659 97.2 globlastp 1976 LNU501maize|gb170|BM382690 3438 659 97.2 globlastp 1977 LNU501foxtail_millet|10v2|FXTRMSLX00598869D2_P1 3439 659 96.6 globlastp 1978LNU501 maize|10v1|DR817878_P1 3440 659 96.6 globlastp 1979 LNU501maize|gb170|DR817878 3440 659 96.6 globlastp 1980 LNU501rice|gb170|OS06G45280 3441 659 92.3 globlastp 1981 LNU501brachypodium|09v1|GT799485_P1 3442 659 91.1 globlastp 1982 LNU501wheat|10v2|BE605194_P1 3443 659 90.1 globlastp 1983 LNU501wheat|gb164|BE605194 3444 659 89.7 globlastp 1984 LNU501barley|10v1|AJ436214 3445 659 87.6 globlastp 1985 LNU501barley|10v2|AJ436214_P1 3445 659 87.6 globlastp 1986 LNU501millet|10v1|EVO454PM011670_P1 3446 659 85.3 globlastp 1987 LNU502wheat|10v2|BE430987_T1 3447 660 93.45 glotblastn 1988 LNU502wheat|gb164|BE430987 3448 660 93 globlastp 1989 LNU502rice|gb170|OS02G32980 3449 660 85.2 globlastp 1990 LNU502foxtail_millet|10v2|SICRP016205_P1 3450 660 84.8 globlastp 1991 LNU502millet|10v1|CD724444_P1 3451 660 84.8 globlastp 1992 LNU502brachypodium|09v1|SRR031797S0004957_P1 3452 660 83.9 globlastp 1993LNU502 maize|10v1|AW165596_P1 3453 660 80.8 globlastp 1994 LNU502maize|gb170|AW165596 3453 660 80.8 globlastp 1995 LNU503lovegrass|gb167|EH188332_P1 3454 661 86 globlastp 1996 LNU503switchgrass|gb167|FL878118 3455 661 85 globlastp 1997 LNU503foxtail_millet|10v2|SICRP010281_P1 3456 661 84.1 globlastp 1998 LNU503sorghum|09v1|SB06G028260 3457 661 84.1 globlastp 1999 LNU503switchgrass|gb167|FL844303 3458 661 84.1 globlastp 2000 LNU503millet|10v1|EVO454PM635012_P1 3459 661 84.1 globlastp 2001 LNU503cynodon|10v1|ES306614_P1 3460 661 83.2 globlastp 2002 LNU503millet|09v1|EVO454PM652594 3461 661 83.18 glotblastn 2003 LNU503sugarcane|10v1|CA269511 3462 661 83.18 glotblastn 2004 LNU503cynodon|10v1|ES306841_P1 3463 661 82.2 globlastp 2005 LNU503brachypodium|09v1|DV473278_P1 3464 661 82.2 globlastp 2006 LNU503oat|10v2|GR350976_P1 3465 661 81.3 globlastp 2007 LNU503maize|10v1|AI637049_P1 3466 661 81.3 globlastp 2008 LNU503maize|gb170|AI637049 3466 661 81.3 globlastp 2009 LNU503maize|gb170|CF633199 3467 661 81.3 globlastp 2010 LNU503maize|gb170|LLDQ245256 3468 661 81.3 globlastp 2011 LNU503wheat|gb164|BQ483317 3468 661 81.3 globlastp 2012 LNU503wheat|gb164|CK213240 3468 661 81.3 globlastp 2013 LNU503wheat|10v2|BQ483317_P1 3468 661 81.3 globlastp 2014 LNU503maize|10v1|CF633199_P1 3469 661 80.4 globlastp 2015 LNU503barley|10v1|AV910573 3470 661 80.4 globlastp 2016 LNU503barley|10v2|AV910573_P1 3470 661 80.4 globlastp 2017 LNU503lolium|10v1|DT670198_P1 3471 661 80.4 globlastp 2018 LNU507barley|10v1|BQ660103 3472 664 89.4 globlastp 2019 LNU507wheat|gb164|BE425628 3473 664 81 globlastp 2020 LNU507leymus|gb166|EG379808_P1 3474 664 80.3 globlastp 2021 LNU507wheat|10v2|BE425628_T1 3475 664 80.29 glotblastn 2022 LNU510maize|10v1|CO519985_P1 3476 667 82.4 globlastp 2023 LNU510brachypodium|09v1|GT849245_P1 3477 667 80.2 globlastp 2024 LNU510sorghum|09v1|SB10G014190 3478 667 80.2 glotblastn 2025 LNU510rice|gb170|OS06G29994 3479 667 80 globlastp 2026 LNU512arabidopsis_lyrata|09v1|JGIAL012417_P1 3480 669 83 globlastp 2027 LNU513soybean|11v1|GLYMA03G34940_P1 3481 670 89.4 globlastp 2028 LNU513soybean|gb168|BU547595 3481 670 89.4 globlastp 2029 LNU514switchgrass|gb167|FE640485 3482 671 91 globlastp 2030 LNU514foxtail_millet|10v2|SICRP005477_P1 3483 671 90.2 globlastp 2031 LNU514sorghum|09v1|SB07G024530 3484 671 89.8 globlastp 2032 LNU514maize|10v1|AI902049_P1 3485 671 89.2 globlastp 2033 LNU514maize|10v1|AI987368_P1 3486 671 88.9 globlastp 2034 LNU514maize|gb170|AI987368 3486 671 88.9 globlastp 2035 LNU514maize|gb170|AI711932 3487 671 88.54 glotblastn 2036 LNU514oat|10v2|GO582307_P1 3488 671 87.5 globlastp 2037 LNU514oat|10v1|GO582307 3488 671 87.5 globlastp 2038 LNU514rice|gb170|OS09G38530 3489 671 86.8 globlastp 2039 LNU514wheat|gb164|BE414509 3490 671 86.8 globlastp 2040 LNU514wheat|10v2|BE414509_P1 3491 671 86.7 globlastp 2041 LNU514barley|10v1|AV833241 3492 671 86.4 globlastp 2042 LNU514barley|10v2|AV833241_P1 3492 671 86.4 globlastp 2043 LNU514brachypodium|09v1|DV481367_P1 3493 671 86.2 globlastp 2044 LNU514maize|10v1|AI677621_P1 3494 671 85.9 globlastp 2045 LNU514maize|gb170|AI677621 3494 671 85.9 globlastp 2046 LNU514sorghum|09v1|SB02G032530 3495 671 85.8 globlastp 2047 LNU514foxtail_millet|10v2|FXTRMSLX00130007D1_P1 3496 671 85 globlastp 2048LNU514 maize|10v1|BQ295771_P1 3497 671 84.8 globlastp 2049 LNU514millet|10v1|EVO454PM005967_P1 3498 671 84.5 globlastp 2050 LNU514sorghum|09v1|SB07G016310 3499 671 82.4 globlastp 2051 LNU514sugarcane|10v1|BU102774 3500 671 82.2 globlastp 2052 LNU514brachypodium|09v1|DV480469_P1 3501 671 81.9 globlastp 2053 LNU514maize|10v1|AI861629_P1 3502 671 81.9 globlastp 2054 LNU514millet|10v1|EVO454PM001017_P1 3503 671 81.8 globlastp 2055 LNU514barley|10v1|BE412814 3504 671 81.14 glotblastn 2056 LNU514switchgrass|gb167|FE600426 3505 671 81.1 globlastp 2057 LNU514barley|10v2|BE412814_P1 3506 671 80.8 globlastp 2058 LNU514wheat|10v2|BF292545_P1 3507 671 80.8 globlastp 2059 LNU514wheat|gb164|BQ238027 3507 671 80.8 globlastp 2060 LNU514millet|10v1|EVO454PM002776_P1 3508 671 80.7 globlastp 2061 LNU514cotton|10v2|SRR032367S0498385_P1 3509 671 80.1 globlastp 2062 LNU517bean|gb167|CA914436_P1 3510 672 90.6 globlastp 2063 LNU517cowpea|gb166|FF383642_P1 3511 672 89.7 globlastp 2064 LNU517lotus|09v1|BP070981_P1 3512 672 87.5 globlastp 2065 LNU517medicago|09v1|LLAW776024_P1 3513 672 83.3 globlastp 2066 LNU517peanut|10v1|ES723257_T1 3514 672 80.66 glotblastn 2067 LNU518sorghum|09v1|SB10G005570 3515 673 95.5 globlastp 2068 LNU518switchgrass|gb167|FE640709 3516 673 93.4 globlastp 2069 LNU518millet|10v1|EVO454PM012014_P1 3517 673 89.5 globlastp 2070 LNU518rice|gb170|OS06G08400 3518 673 86.8 globlastp 2071 LNU518wheat|10v2|BE493248_P1 3519 673 86.1 globlastp 2072 LNU518wheat|gb164|BE493248 3520 673 86.1 globlastp 2073 LNU518brachypodium|09v1|GT786826_P1 3521 673 85.5 globlastp 2074 LNU518brachypodium|09v1|GT759144_P1 3522 673 85 globlastp 2075 LNU520maize|10v1|BG320595_P1 3523 675 92.1 globlastp 2076 LNU520maize|gb170|BG549573 3523 675 92.1 globlastp 2077 LNU520switchgrass|gb167|FE612728 3524 675 90.03 glotblastn 2078 LNU520millet|10v1|EVO454PM312756_P1 3525 675 81.6 globlastp 2079 LNU417_H4millet|10v1|EVO454PM015064_P1 3526 677 83.1 globlastp 2079 LNU417millet|10v1|EVO454PM015064_T1 3526 702 84.38 glotblastn 2080 LNU290barley|10v2|AV836409_T1 3527 680 82.59 glotblastn 2081 LNU290wheat|10v2|DR737283_T1 3528 680 81.53 glotblastn 2082 LNU294soybean|11v1|GLYMA02G03460_T1 3529 681 83.56 glotblastn 2083 LNU309switchgrass|gb167|DN147382 3530 684 86.82 glotblastn 2084 LNU337citrus|gb166|CN183940_T1 3531 686 86.36 glotblastn 2085 LNU337aquilegia|10v1|DR938015 3532 686 85.54 glotblastn 2086 LNU337aquilegia|10v2|DR938015_T1 3533 686 85.54 glotblastn 2087 LNU337poplar|10v1|AI166531_T1 3534 686 84.68 glotblastn 2088 LNU337cassava|09v1|DB955139_T1 3535 686 84.66 glotblastn 2089 LNU337cotton|10v1|BQ408171 3536 686 84.57 glotblastn 2090 LNU337soybean|11v1|GLYMA08G20750_T1 3537 686 84.5 glotblastn 2091 LNU337soybean|gb168|CX532836 3537 686 84.5 glotblastn 2092 LNU337soybean|gb168|BM779948 3538 686 84.45 glotblastn 2093 LNU337lotus|09v1|GO036990_T1 3539 686 84.1 glotblastn 2094 LNU337cowpea|gb166|FF383005_P1 3540 686 83.9 globlastp 2095 LNU337aristolochia|10v1|FD748181_T1 3541 686 83.74 glotblastn 2096 LNU337cotton|10v2|BQ408171_T1 3542 686 83.64 glotblastn 2097 LNU337medicago|09v1|LLAW696817_T1 3543 686 83.38 glotblastn 2098 LNU337artemisia|10v1|EY088616_T1 3544 686 83.33 glotblastn 2099 LNU337solanum_phureja|09v1|SPHAI488887 3545 686 83.13 glotblastn 2100 LNU337cucumber|09v1|CK086034_T1 3546 686 82.87 glotblastn 2101 LNU337pigeonpea|10v1|SRR054580S0020598_T1 3547 686 82.52 glotblastn 2102LNU337 tomato|09v1|AI488887 3548 686 82.23 glotblastn 2103 LNU337eucalyptus|11v1|SRR001659X140003_T1 3549 686 82.21 glotblastn 2104LNU337 soybean|gb168|AW693235 3550 686 81.27 glotblastn 2105 LNU337amaranthus|10v1|SRR039411S0002870_T1 3551 686 81.17 glotblastn 2106LNU337 sunflower|10v1|DY921887_T1 3552 686 81.17 glotblastn 2107 LNU337sunflower|gb162|DY921887 3553 686 81.17 glotblastn 2108 LNU337solanum_phureja|09v1|SPHBI923775 3554 686 80.55 glotblastn 2109 LNU337tomato|09v1|BI923775 3555 686 80.43 glotblastn 2110 LNU337triphysaria|10v1|EY168040 3556 686 80.25 glotblastn 2111 LNU337bean|gb167|CV538438_T1 3557 686 80.24 glotblastn 2112 LNU350wheat|gb164|BE398679 3558 688 99.1 globlastp 2113 LNU350pseudoroegneria|gb167|FF340338 3559 688 94.3 globlastp 2114 LNU350leymus|gb166|CN466395_P1 3560 688 93.7 globlastp 2115 LNU350oat|10v2|GO591066_P1 3561 688 88.1 globlastp 2116 LNU350rice|gb170|OS10G35520 3562 688 82.4 globlastp 2117 LNU369pseudoroegneria|gb167|FF340190 3563 691 100 glotblastn 2118 LNU369barley|10v1|BQ664541 3564 691 96.4 globlastp 2119 LNU369barley|10v2|BQ664541_P1 3565 691 95.9 globlastp 2120 LNU369brachypodium|09v1|TMPLBQ664541T1_P1 3566 691 95.9 globlastp 2121 LNU369millet|09v1|EVO454PM068764 3567 691 91.67 glotblastn 2122 LNU369sugarcane|10v1|CA088432 3568 691 89.29 glotblastn 2123 LNU369sorghum|09v1|SB03G044420 3569 691 88.1 glotblastn 2124 LNU369fescue|gb161|DT686802_P1 3570 691 86.7 globlastp 2125 LNU369maize|gb170|EG299620 3571 691 85.12 glotblastn 2126 LNU369foxtail_millet|10v2|FXTRMSLX00024110D1_P1 3572 691 85 globlastp 2127LNU380 brachypodium|09v1|SRR031795S0001004_T1 3573 695 90.62 glotblastn2128 LNU380 oat|10v2|GR352653_P1 3574 695 84.9 globlastp 2129 LNU380rice|gb170|OS05G40770 3575 695 82.96 glotblastn 2130 LNU380maize|10v1|BQ280303_T1 3576 695 81.11 glotblastn 2131 LNU380maize|gb170|BQ280303 3577 695 81.11 glotblastn 2132 LNU380sorghum|09v1|SB09G023780 3578 695 80.93 glotblastn 2133 LNU401maize|gb170|AI396396 3579 696 92.7 globlastp 2134 LNU407wheat|gb164|CA709529 3580 697 96.31 glotblastn 2135 LNU407foxtail_millet|10v2|FXTRMSLX00545432D1_T1 3581 697 84.84 glotblastn 2136LNU407 millet|10v1|EVO454PM021192_T1 3582 697 82.79 glotblastn 2137LNU407 millet|09v1|EVO454PM021192 3583 697 82.79 glotblastn 2138 LNU407sorghum|09v1|SB09G023570 3584 697 80.33 glotblastn 2139 LNU409wheat|10v2|CA651811_T1 3585 698 96.45 glotblastn 2140 LNU409wheat|gb164|CA651811 3585 698 96.45 glotblastn 2141 LNU409brachypodium|09v1|SRR031797S0045274_T1 3586 698 89.36 glotblastn 2142LNU409 sorghum|09v1|SB09G017200 3587 698 87.94 glotblastn 2143 LNU409rice|gb170|OS05G28830 3588 698 80.99 glotblastn 2144 LNU410barley|10v1|BF624533 3589 699 96.7 globlastp 2145 LNU410barley|10v2|BF624533_P1 3589 699 96.7 globlastp 2146 LNU414barley|10v1|BE413415 3590 700 90.91 glotblastn 2147 LNU414foxtail_millet|10v2|SICRP039145_T1 3591 700 82.64 glotblastn 2148 LNU414switchgrass|gb167|FE635772 3592 700 82.64 glotblastn 2149 LNU417millet|09v1|EVO454PM015064 3593 702 84.11 glotblastn 2150 LNU417millet|09v1|EVO454PM040968 3594 702 83.3 globlastp 2151 LNU453sorghum|09v1|SB10G027370 3595 703 81.46 glotblastn 2152 LNU457solanum_phureja|09v1|SPHBQ512773 3596 704 96.34 glotblastn 2153 LNU457potato|10v1|GFXAY165021X1_T1 3597 704 84.82 glotblastn 2154 LNU457monkeyflower|10v1|MGJGI019441_T1 3598 704 80.63 glotblastn 2155 LNU466millet|10v1|EVO454PM011905_T1 3599 705 89.7 glotblastn 2156 LNU466millet|09v1|EVO454PM011905 3600 705 88.84 glotblastn 2157 LNU466sorghum|09v1|SB08G019390 3601 705 88.84 glotblastn 2158 LNU466sugarcane|10v1|CA066125 3602 705 88.41 glotblastn 2159 LNU466oat|10v2|GR353248_T1 3603 705 86.38 glotblastn 2160 LNU466foxtail_millet|10v2|SICRP027225_T1 3604 705 84.98 glotblastn 2161 LNU466maize|10v1|AW179506_T1 3605 705 83.05 glotblastn 2162 LNU466maize|gb170|AW179506 3605 705 83.05 glotblastn 2163 LNU466switchgrass|gb167|FE654167 3606 705 81.97 glotblastn 2164 LNU466pseudoroegneria|gb167|FF351252 3607 705 80.93 glotblastn 2165 LNU466fescue|gb161|DT679702_T1 3608 705 80 glotblastn 2166 LNU474bean|gb167|CV536461_T1 3609 707 98.36 glotblastn 2167 LNU474cowpea|gb166|FC462110_T1 3610 707 98.36 glotblastn 2168 LNU474liquorice|gb171|FS241287_T1 3611 707 90.16 glotblastn 2169 LNU474lotus|09v1|LLBW600621_T1 3612 707 90.16 glotblastn 2170 LNU474peanut|10v1|ES719423_T1 3613 707 88.52 glotblastn 2171 LNU474prunus|10v1|DY636612_T1 3614 707 86.89 glotblastn 2172 LNU474prunus|gb167|DY636612 3615 707 86.89 glotblastn 2173 LNU474apple|gb171|DT003448_T1 3616 707 83.61 glotblastn 2174 LNU474clover|gb162|BB909259_T1 3617 707 83.61 glotblastn 2175 LNU474nasturtium|10v1|SRR032559S0001264_T1 3618 707 83.61 glotblastn 2176LNU474 petunia|gb171|FN012889_T1 3619 707 83.61 glotblastn 2177 LNU474clementine|11v1|CF505635_T1 3620 707 81.97 glotblastn 2178 LNU474oak|10v1|FP043396_T1 3621 707 81.97 glotblastn 2179 LNU474orange|11v1|CF505635_T1 3622 707 81.97 glotblastn 2180 LNU474canola|10v1|DY002167_T1 3623 707 81.97 glotblastn 2181 LNU474castorbean|09v1|EG665732_T1 3624 707 81.97 glotblastn 2182 LNU474citrus|gb166|CF505635_T1 3625 707 81.97 glotblastn 2183 LNU474cucumber|09v1|CSCRP015980_T1 3626 707 81.97 glotblastn 2184 LNU474thellungiella|gb167|BY828100 3627 707 81.97 glotblastn 2185 LNU474arabidopsis_lyrata|09v1|JGIAL011182_T1 3628 707 80.33 glotblastn 2186LNU474 arabidopsis|10v1|AT3G24520_T1 3629 707 80.33 glotblastn 2187LNU474 chestnut|gb170|SRR006300S0039964_T1 3630 707 80.33 glotblastn2188 LNU474 grape|gb160|CB920522_T1 3631 707 80.33 glotblastn 2189LNU474 strawberry|11v1|CX309755_T1 3632 707 80.33 glotblastn 2190 LNU474strawberry|gb164|EX668883 3633 707 80.33 glotblastn 2191 LNU487barley|10v2|BE558461_T1 3634 708 97.73 glotblastn 2192 LNU500grape|gb160|CB915642_P1 3635 711 86.8 globlastp 2193 LNU500grape|gb160|CB972116_T1 3636 711 86.4 glotblastn 2194 LNU500radish|gb164|EV547025 3637 711 85.9 globlastp 2195 LNU500orobanche|10v1|SRR023189S0037471_P1 3638 711 85.1 globlastp 2196 LNU500clementine|11v1|CX073917_T1 3639 711 85.09 glotblastn 2197 LNU500ipomoea_nil|10v1|CJ757665_P1 3640 711 84.9 globlastp 2198 LNU500b_juncea|10v2|CK991428_T1 3641 711 84.65 glotblastn 2199 LNU500cacao|10v1|CU499397_T1 3642 711 84.65 glotblastn 2200 LNU500pigeonpea|10v1|SRR054580S0019557_T1 3643 711 84.65 glotblastn 2201LNU500 cotton|10v2|SRR032367S0333890_T1 3644 711 84.65 glotblastn 2202LNU500 spurge|gb161|DV119836 3645 711 84.4 globlastp 2203 LNU500arabidopsis|10v1|AT5G11880_T1 3646 711 84.21 glotblastn 2204 LNU500artemisia|10v1|EY080298_T1 3647 711 84.21 glotblastn 2205 LNU500artemisia|10v1|EY080299_T1 3648 711 84.21 glotblastn 2206 LNU500cleome_gynandra|10v1|SRR015532S0016904_T1 3649 711 84.21 glotblastn 2207LNU500 soybean|11v1|GLYMA13G20390_T1 3650 711 84.21 glotblastn 2208LNU500 soybean|gb168|AL372335 3650 711 84.21 glotblastn 2209 LNU500radish|gb164|EX769006 3651 711 83.93 glotblastn 2210 LNU500amaranthus|10v1|SRR039411S0005472_T1 3652 711 83.77 glotblastn 2211LNU500 arabidopsis_lyrata|09v1|JGIAL020888_T1 3653 711 83.77 glotblastn2212 LNU500 artemisia|10v1|EY072335_T1 3654 711 83.77 glotblastn 2213LNU500 canola|10v1|CD822749_T1 3655 711 83.77 glotblastn 2214 LNU500cleome_spinosa|10v1|SRR015531S0019032_T1 3656 711 83.33 glotblastn 2215LNU500 cowpea|gb166|FC461450_T1 3657 711 83.33 glotblastn 2216 LNU500momordica|10v1|SRR071315S0003699_T1 3658 711 83.04 glotblastn 2217LNU500 cynara|gb167|GE579895_T1 3659 711 82.89 glotblastn 2218 LNU500sunflower|10v1|CX947049_T1 3660 711 82.89 glotblastn 2219 LNU500clover|gb162|BB905074_P1 3661 711 82.3 globlastp 2220 LNU500aristolochia|10v1|SRR039082S0276748_T1 3662 711 82.02 glotblastn 2221LNU500 papaya|gb165|EX238932_T1 3663 711 82.02 glotblastn 2222 LNU500prunus|gb167|BU041335 3664 711 82.02 glotblastn 2223 LNU500lotus|09v1|LLCB826869_P1 3665 711 81.8 globlastp 2224 LNU500strawberry|11v1|GT150985_T1 3666 711 81.58 glotblastn 2225 LNU500apple|gb171|CN877675_T1 3667 711 81.58 glotblastn 2226 LNU500peanut|10v1|EG029135_T1 3668 711 81.14 glotblastn 2227 LNU500peanut|gb171|EG029135 3669 711 81.14 glotblastn 2228 LNU500radish|gb164|EW714733 3670 711 80.5 globlastp 2229 LNU506solanum_phureja|09v1|SPHAI490778 3671 713 92.7 globlastp 2230 LNU310solanum_phureja|09v1|SPHBG133786 3672 721 97 globlastp 2231 LNU310potato|10v1|BI177611_P1 3673 721 96.3 globlastp 2232 LNU310eggplant|10v1|FS048892_P1 3674 721 93.3 globlastp 2233 LNU323solanum_phureja|09v1|SPHBG626676 3675 722 86.72 glotblastn 2234 LNU323potato|10v1|CV502122_P1 3676 722 86.7 globlastp 2235 LNU323solanum_phureja|09v1|SPHBG631554 3677 722 85.16 glotblastn 2236 LNU326potato|10v1|BG589666_P1 3678 724 95.3 globlastp 2237 LNU326solanum_phureja|09v1|SPHBG126891 3679 724 94.7 globlastp 2238 LNU326eggplant|10v1|FS016668_P1 3680 724 86.3 globlastp 2239 LNU326pepper|gb171|AA840658_P1 3681 724 86.1 globlastp 2240 LNU326tobacco|gb162|AJ718732 3682 724 82.66 glotblastn 2241 LNU326nicotiana_benthamiana|gb162| 3683 724 80.79 glotblastn CN743291_T1 2242LNU329 potato|10v1|BG589552_P1 3684 726 94.6 globlastp 2243 LNU329solanum_phureja|09v1|SPHBG791244 3685 726 94.6 globlastp 2244 LNU329pepper|gb171|CA518152_P1 3686 726 86.3 globlastp 2245 LNU329petunia|gb171|DW177095_T1 3687 726 81.65 glotblastn 2246 LNU331nicotiana_benthamiana|gb162| 3688 727 89.6 globlastp CK290936_P1 2247LNU335 oat|10v2|CN814905_P1 3689 728 83 globlastp 2248 LNU350barley|10v1|BE216626 3690 732 94.7 globlastp 2249 LNU350barley|10v2|BE216626_P1 3690 732 94.7 globlastp 2250 LNU350brachypodium|09v1|GT819129_P1 3691 732 86.4 globlastp 2251 LNU350sorghum|09v1|SB05G003700 3692 732 86.1 globlastp 2252 LNU350sugarcane|10v1|CA073962 3693 732 85.2 globlastp 2253 LNU360wheat|10v2|BQ905138_P1 3694 733 84.2 globlastp 2254 LNU360wheat|gb164|BQ905138 3694 733 84.2 globlastp 2255 LNU360barley|10v1|BE421126 3695 733 83.3 globlastp 2256 LNU360barley|10v2|BE421126_P1 3695 733 83.3 globlastp 2257 LNU360wheat|10v2|BF478587_P1 3696 733 83.3 globlastp 2258 LNU360wheat|gb164|BF478587 3696 733 83.3 globlastp 2259 LNU360wheat|10v2|BE418197_P1 3697 733 83.3 globlastp 2260 LNU360wheat|gb164|BE418197 3697 733 83.3 globlastp 2261 LNU360pseudoroegneria|gb167|FF341642 3698 733 82.9 globlastp 2262 LNU368wheat|10v2|BE400013_P1 3699 735 96 globlastp 2263 LNU368leymus|gb166|EG394955_P1 3700 735 94.4 globlastp 2264 LNU368wheat|gb164|BE400013 3701 735 89.7 globlastp 2265 LNU368barley|10v1|BE421103 3702 735 89.1 globlastp 2266 LNU368barley|10v2|BE421103_P1 3702 735 89.1 globlastp 2267 LNU368pseudoroegneria|gb167|FF346438 3703 735 86.8 globlastp 2268 LNU372leymus|gb166|EG379844_P1 3704 737 88.6 globlastp 2269 LNU384solanum_phureja|09v1|SPHAI482780 3705 741 94.9 globlastp 2270 LNU384tobacco|gb162|EB444563 3706 741 80.1 globlastp 2271 LNU397sugarcane|10v1|CA114434 3707 745 91.6 globlastp 2272 LNU397maize|10v1|AI691183_P1 3708 745 91.1 globlastp 2273 LNU397maize|gb170|AI691183 3708 745 91.1 globlastp 2274 LNU401switchgrass|gb167|FE645149_P1 3709 746 80.7 globlastp 2275 LNU407wheat|10v2|BQ245199_P1 3710 749 97.3 globlastp 2276 LNU407brachypodium|09v1|DV473592_P1 3711 749 89.9 globlastp 2277 LNU407sorghum|09v1|SB03G038110 3712 749 86 globlastp 2278 LNU407rice|gb170|OS01G60330 3713 749 84.8 globlastp 2279 LNU407maize|10v1|DN559520_P1 3714 749 83.9 globlastp 2280 LNU407maize|gb170|DN559520 3714 749 83.9 globlastp 2281 LNU407maize|10v1|AI621549_P1 3715 749 83.2 globlastp 2282 LNU416b_rapa|gb162|BG543823_P1 754 754 100 globlastp 2283 LNU416canola|10v1|CD827516_P1 754 754 100 globlastp 2284 LNU416b_oleracea|gb161|AM396074_P1 3716 754 95.9 globlastp 2285 LNU416canola|10v1|EV117448_T1 3717 754 86.59 glotblastn 2286 LNU416canola|10v1|CD818961_P1 3718 754 85.7 globlastp 2287 LNU416b_oleracea|gb161|DQ059298_P1 3719 754 85.1 globlastp 2288 LNU416canola|10v1|CD834758_P1 3720 754 81 globlastp 2289 LNU419solanum_phureja|09v1|SPHBG132251 3721 755 99.5 globlastp 2290 LNU419potato|10v1|BE922576_P1 3722 755 99.2 globlastp 2291 LNU419solanum_phureja|09v1|SPHBE922576 3723 755 97.9 globlastp 2292 LNU419tobacco|gb162|AB004307 3724 755 95.2 globlastp 2293 LNU419nicotiana_benthamiana|gb162| 3725 755 94.7 globlastp CK295383_P1 2294LNU419 eggplant|10v1|FS003533_P1 3726 755 93.9 globlastp 2295 LNU419pepper|gb171|AF108885_P1 3727 755 89.3 globlastp 2296 LNU419cucumber|09v1|AM714300_P1 3728 755 83.6 globlastp 2297 LNU419bean|gb167|CA901472_P1 3729 755 83.6 globlastp 2298 LNU419cowpea|gb166|FF390066_P1 3730 755 83.6 globlastp 2299 LNU419melon|10v1|AM714300_P1 3731 755 83.4 globlastp 2300 LNU419eucalyptus|11v1|CT981021_P1 3732 755 83.3 globlastp 2301 LNU419momordica|10v1|SRR071315S0000520_P1 3733 755 83.3 globlastp 2302 LNU419melon|gb165|AM714300 3734 755 83.1 globlastp 2303 LNU419pigeonpea|10v1|SRR054580S0001030_P1 3735 755 82.8 globlastp 2304 LNU419peanut|10v1|CD038560_P1 3736 755 82.5 globlastp 2305 LNU419soybean|11v1|GLYMA15G13680_P1 3737 755 82.5 globlastp 2306 LNU419soybean|gb168|BE352683 3737 755 82.5 globlastp 2307 LNU419orange|11v1|CF504082_P1 3738 755 82.3 globlastp 2308 LNU419peanut|10v1|GO326838_P1 3739 755 82.2 globlastp 2309 LNU419prunus|10v1|CN488554_P1 3740 755 82 globlastp 2310 LNU419cassava|09v1|DV441828_P1 3741 755 82 globlastp 2311 LNU419castorbean|09v1|EE256160_P1 3742 755 82 globlastp 2312 LNU419oak|10v1|FN696815_P1 3743 755 81.7 globlastp 2313 LNU419cassava|09v1|CK650384_P1 3744 755 81.7 globlastp 2314 LNU419soybean|11v1|GLYMA09G02800_P1 3745 755 81.7 globlastp 2315 LNU419soybean|gb168|AW171758 3745 755 81.7 globlastp 2316 LNU419apple|gb171|CN488554_P1 3746 755 81.5 globlastp 2317 LNU419kiwi|gb166|FG409924_P1 3747 755 81.4 globlastp 2318 LNU419aristolochia|10v1|FD759327_P1 3748 755 81.2 globlastp 2319 LNU419nasturtium|10v1|GH170410_T1 3749 755 80.95 glotblastn 2320 LNU419cotton|10v1|BF269486 3750 755 80.6 globlastp 2321 LNU419tragopogon|10v1|SRR020205S0004523 3751 755 80.6 globlastp 2322 LNU419aquilegia|10v2|DR925602_P1 3752 755 80.4 globlastp 2323 LNU419poplar|10v1|BI072464_P1 3753 755 80.4 globlastp 2324 LNU419prunus|gb167|CV044964 3754 755 80.37 glotblastn 2325 LNU419artemisia|10v1|EY066317_T1 3755 755 80.16 glotblastn 2326 LNU419orobanche|10v1|SRR023189S0003219_P1 3756 755 80.1 globlastp 2327 LNU419cotton|10v2|BF275008_P1 3757 755 80.1 globlastp 2328 LNU439maize|gb170|AW574419 3758 757 87.8 globlastp 2329 LNU442solanum_phureja|09v1|SPHAW735755 3759 758 94.5 globlastp 2330 LNU444cacao|10v1|CA795284_P1 3760 759 87.8 globlastp 2331 LNU444cacao|gb167|CA795284 3760 759 87.8 globlastp 2332 LNU444poplar|10v1|AI162462_P1 3761 759 85.8 globlastp 2333 LNU444oak|10v1|FP024990_P1 3762 759 84.6 globlastp 2334 LNU444oak|10v1|FP025793_P1 3762 759 84.6 globlastp 2335 LNU444papaya|gb165|EX260629_P1 3763 759 84.5 globlastp 2336 LNU444cassava|09v1|CK641349_P1 3764 759 83.8 globlastp 2337 LNU444flax|09v1|EU829138_P1 3765 759 83.6 globlastp 2338 LNU444flax|09v1|CV478267_P1 3766 759 82.7 globlastp 2339 LNU444nasturtium|10v1|SRR032558S0005447_P1 3767 759 81.9 globlastp 2340 LNU444bruguiera|gb166|BP939110_P1 3768 759 80.5 globlastp 2341 LNU444prunus|10v1|CN491505_P1 3769 759 80.4 globlastp 2342 LNU444chickpea|09v2|EL585362_P1 3770 759 80.1 globlastp 2343 LNU450cacao|10v1|CU471751_P1 3771 763 93 globlastp 2344 LNU450cassava|09v1|JGICASSAVA878VALIDM1_P1 3772 763 86.1 globlastp 2345 LNU450castorbean|09v1|XM002510536_P1 3773 763 83.1 globlastp 2346 LNU450grape|gb160|CB007771_P1 3774 763 82.8 globlastp 2347 LNU450orange|11v1|CX546774_P1 3775 763 81.9 globlastp 2348 LNU450clementine|11v1|CX546774_P1 3776 763 81.4 globlastp 2349 LNU450tea|10v1|CV699613 3777 763 81.4 globlastp 2350 LNU450prunus|10v1|CB823756_P1 3778 763 80.3 globlastp 2351 LNU450eucalyptus|11v1|CD670135_P1 3779 763 80 globlastp 2352 LNU461solanum_phureja|09v1|SPHAI483350 3780 766 96.8 globlastp 2353 LNU465maize|gb170|LLEY954018 3781 768 81.1 globlastp 2354 LNU468eggplant|10v1|FS007833_P1 3782 769 94.2 globlastp 2355 LNU470wheat|10v2|CJ925970_P1 3783 770 94.4 globlastp 2356 LNU470oat|10v2|GR345351_P1 3784 770 84.9 globlastp 2357 LNU470oat|10v1|GR345351 3784 770 84.9 globlastp 2358 LNU471sugarcane|10v1|CA095155_P1 3785 771 80.1 globlastp 2359 LNU472brachypodium|09v1|SRR031796S0007593_P1 3786 772 88.6 globlastp 2360LNU472 rice|gb170|OS04G58380 3787 772 85 globlastp 2361 LNU472switchgrass|gb167|FL771162 3788 772 83.8 globlastp 2362 LNU472maize|10v1|AW927938_P1 3789 772 81.4 globlastp 2363 LNU472maize|gb170|AW927938 3789 772 81.4 globlastp 2364 LNU472sugarcane|10v1|CA231840 3790 772 80.7 globlastp 2365 LNU474soybean|11v1|GLYMA07G09520_P1 3791 773 99.7 globlastp 2366 LNU474soybean|gb168|BE347442 3792 773 88.8 globlastp 2367 LNU474soybean|11v1|GLYMA09G32300_P1 3793 773 88.5 globlastp 2368 LNU476maize|10v1|AW461103_P1 3794 774 92.5 globlastp 2369 LNU476maize|gb170|AW461103 3794 774 92.5 globlastp 2370 LNU476sugarcane|10v1|CA067184 3795 774 90.4 globlastp 2371 LNU476sorghum|09v1|SB02G036750 3796 774 89.8 globlastp 2372 LNU476foxtail_millet|10v2|OXFXTRMSLX00112582D1T1_P1 3797 774 85.5 globlastp2373 LNU476 millet|10v1|CD725707_T1 3798 774 84.29 glotblastn 2374LNU476 switchgrass|gb167|DN148685 3799 774 82.2 globlastp 2375 LNU495maize|10v1|AI622661_P1 3800 777 92.1 globlastp 2376 LNU495maize|10v1|BG321733_P1 3801 777 90.6 globlastp 2377 LNU495brachypodium|09v1|GT758308_P1 3802 777 84.3 globlastp 2378 LNU495barley|10v2|BJ451039_P1 3803 777 83 globlastp 2379 LNU499wheat|gb164|BE497147 3804 779 92.8 globlastp 2380 LNU504arabidopsis_lyrata|09v1|JGIAL012450_T1 3805 780 91.49 glotblastn 2381LNU507 barley|10v2|BF629582_P1 3806 781 97.8 globlastp 2382 LNU507wheat|10v2|BE401116_P1 3807 781 90 globlastp 2383 LNU507wheat|gb164|BE401116 3807 781 90 globlastp 2384 LNU507wheat|gb164|BE425320 3808 781 89.6 globlastp 2385 LNU507leymus|gb166|CN466143_P1 3809 781 89.6 globlastp 2386 LNU507wheat|10v2|BE425320_P1 3808 781 89.6 globlastp 2387 LNU507wheat|gb164|BE426025 3810 781 88.93 glotblastn 2388 LNU507wheat|gb164|BE414564 3811 781 83.9 globlastp 2389 LNU507wheat|10v2|BE414564_P1 3812 781 82.9 globlastp 2390 LNU507wheat|10v2|BE399826_P1 3813 781 82.5 globlastp 2391 LNU507wheat|gb164|BE399826 3813 781 82.5 globlastp 2392 LNU517soybean|11v1|GLYMA16G08470_P1 3814 783 92.7 globlastp 2393 LNU517soybean|gb168|BF643214 3814 783 92.7 globlastp 2394 LNU519sorghum|09v1|SB04G038440 3815 784 92.5 globlastp 2395 LNU519rice|gb170|OS02G58510 3816 784 84.9 globlastp 2396 LNU519switchgrass|gb167|FL698539 3817 784 82.2 globlastp 2397 LNU519brachypodium|09v1|GT761258_P1 3818 784 81.1 globlastp Table 2: Providedare the homologous polypeptides and polynucleotides of the genesidentified in Table 1 and of their cloned genes, which can increasenitrogen use efficiency, fertilizer use efficiency, yield, seed yield,growth rate, vigor, biomass, oil content, fiber yield, fiber quality,fiber length, abiotic stress tolerance and/or water use efficiency of aplant. Homology was calculated as % of identity over the alignedsequences. The query sequences were polypeptide sequences SEQ ID NOs:470-716 and 717-784 and the subject sequences are polypeptide sequencesor polynucleotide sequences which were dynamically translated in all sixreading frames identified in the database based on greater than 80%identity to the query polypeptide sequences. “Polyp.” = polypeptide;“Polyn.”—Polynucleotide. Algor. = Algorithm. “globlastp ™”—globalhomology using blastp ™; “glotblastn ™”—global homology using tblastn ™.“Hom.”—homologous.

The output of the functional genomics approach described herein is a setof genes highly predicted to improve nitrogen use efficiency, fertilizeruse efficiency, yield, seed yield, growth rate, vigor, biomass, oilcontent, fiber yield, fiber length, fiber quality, abiotic stresstolerance and/or water use efficiency of a plant by increasing theirexpression.

Although each gene is predicted to have its own impact, modifying themode of expression of more than one gene or gene product (RNA,polypeptide) is expected to provide an additive or synergistic effect onthe desired trait (e.g., nitrogen use efficiency, fertilizer useefficiency, yield, growth rate, vigor, biomass, oil content, abioticstress tolerance and/or water use efficiency of a plant). Altering theexpression of each gene described here alone or of a set of genestogether increases the overall yield and/or other agronomic importanttraits, hence expects to increase agricultural productivity.

Example 3 Production of Arabidopsis Transcriptom and High ThroughputCorrelation Analysis Using 44K Arabidopsis Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparingbetween plant phenotype and gene expression level, the present inventorsutilized a Arabidopsis oligonucleotide micro-array, produced by AgilentTechnologies [Hypertext Transfer Protocol://World Wide Web (dot) chem(dot) agilent (dot) com/Scripts/PDS (dot) asp?lPage=50879]. The arrayoligonucleotide represents about 44,000 Arabidopsis genes andtranscripts. To define correlations between the levels of RNA expressionwith NUE, yield components or vigor related parameters various plantcharacteristics of 14 different Arabidopsis ecotypes were analyzed.Among them, ten ecotypes encompassing the observed variance wereselected for RNA expression analysis. The correlation between the RNAlevels and the characterized parameters was analyzed using Pearsoncorrelation test [Hypertext Transfer Protocol://World Wide Web (dot)davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Arabidopsis tissues—Two tissues of plants [leaves and stems]growing at two different nitrogen fertilization levels (1.5 mM Nitrogenor 6 mM Nitrogen) were sampled and RNA was extracted as described above.Each micro-array expression information tissue type has received a SetID as summarized Table 3 below.

TABLE 3 Arabidopsis transcriptom expression sets Expression Set Set IDLeaves at 1.5 mM Nitrogen fertilization A Leaves at 6 mM Nitrogenfertilization B Stems at 1.5 mM Nitrogen fertilization C Stem at 6 mMNitrogen fertilization D Table 3.

Arabidopsis yield components and vigor related parameters underdifferent nitrogen fertilization levels assessment—10 Arabidopsisaccessions in 2 repetitive plots each containing 8 plants per plot weregrown in a greenhouse. The growing protocol used was as follows: surfacesterilized seeds were sown in Eppendorf tubes containing 0.5×Murashige-Skoog basal salt medium and grown at 23° C. under 12-hourlight and 12-hour dark daily cycles for 10 days. Then, seedlings ofsimilar size were carefully transferred to pots filled with a mix ofperlite and peat in a 1:1 ratio. Constant nitrogen limiting conditionswere achieved by irrigating the plants with a solution containing 1.5 mMinorganic nitrogen in the form of KNO₃, supplemented with 2 mM CaCl₂,1.25 mM KH₂PO₄, 1.50 mM MgSO₄, 5 mM KCl, 0.01 mM H3B03 andmicroelements, while normal irrigation conditions was achieved byapplying a solution of 6 mM inorganic nitrogen also in the form of KNO₃,supplemented with 2 mM CaCl₂, 1.25 mM KH₂PO₄, 1.50 mM MgSO₄, 0.01 mMH3B03 and microelements. To follow plant growth, trays were photographedthe day nitrogen limiting conditions were initiated and subsequentlyevery 3 days for about 15 additional days. Rosette plant area was thendetermined from the digital pictures. ImageJ software was used forquantifying the plant size from the digital pictures [Hypertext TransferProtocol://rsb (dot) info (dot) nih (dot) gov/ij/] utilizing proprietaryscripts designed to analyze the size of rosette area from individualplants as a function of time. The image analysis system included apersonal desktop computer (Intel P4 3.0 GHz processor) and a publicdomain program—ImageJ 1.37 (Java based image processing program, whichwas developed at the U.S. National Institutes of Health and freelyavailable on the internet [Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/]. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Data parameters collected are summarized in Table 4, herein below.

TABLE 4 Arabidopsis correlated parameters (vectors) Correlated parameterwith Correlation Id N 1.5 mM; Rosette Area at day 8 [cm²] 1 N 1.5 mM;Rosette Area at day 10 [cm²] 2 N 1.5 mM; Plot Coverage at day 8 [%] 3 N1.5 mM; Plot Coverage at day 10 [%] 4 N 1.5 mM; Leaf Number at day 10 5N 1.5 mM; Leaf Blade Area at day 10 [cm²] 6 N 1.5 mM; RGR of RosetteArea at day 3 [cm²/day] 7 N 1.5 mM; t50 Flowering [day] 8 N 1.5 mM; DryWeight [gr./plant] 9 N 1.5 mM; Seed Yield [gr./plant] 10 N 1.5 mM;Harvest Index 11 N 1.5 mM; 1000 Seeds weight [gr.] 12 N 1.5 mM; seedyield/rosette area at day 10 [gr./cm²] 13 N 1.5 mM; seed yield/leafblade [gr./cm²] 14 N 1.5 mM; % Seed yield reduction compared to N 6 mM15 N 1.5 mM; % Biomass reduction compared to N 6 mM 16 N 1.5 mM; Nlevel/DW [SPAD unit/gr.] 17 N 1.5 mM; DW/N level [gr./SPAD unit] 18 N1.5 mM; seed yield/N level [gr./SPAD unit] 19 N 6 mM; Rosette Area atday 8 [cm²] 20 N 6 mM; Rosette Area at day 10 [cm²] 21 N 6 mM; PlotCoverage at day 8 [%] 22 N 6 mM; Plot Coverage at day 10 [%] 23 N 6 mM;Leaf Number at day 10 24 N 6 mM; Leaf Blade Area at day 10 25 N 6 mM;RGR of Rosette Area at day 3 [cm²/gr.] 26 N 6 mM; t50 Flowering [day] 27N 6 mM; Dry Weight [gr./plant] 28 N 6 mM; Seed Yield [gr./plant] 29 N 6mM; Harvest Index 30 N 6 mM; 1000 Seeds weight [gr.] 31 N 6 mM; seedyield/rosette area day at day 10 [gr./cm²] 32 N 6 mM; seed yield/leafblade [gr./cm²] 33 N 6 mM; N level/FW 34 N 6 mM; DW/N level [gr./SPADunit] 35 N 6 mM; N level/DW (SPAD unit/gr. plant) 36 N 6 mM; Seedyield/N unit [gr./SPAD unit] 37 Table 4. “N” = Nitrogen at the notedconcentrations; “cm” = centimeter; “mM” = millimolar; “gr.” = grams;“SPAD” = chlorophyll levels; “t50” = time where 50% of plants flowered;“gr./SPAD unit” = plant biomass expressed in grams per unit of nitrogenin plant measured by SPAD. “DW” = plant dry weight; “N level/DW” = plantNitrogen level measured in SPAD unit per plant biomass [gr.]; “DW/Nlevel” = plant biomass per plant [gr.]/SPAD unit; RGR = relative growthrate;

Assessment of NUE, yield components and vigor-related parameters—TenArabidopsis ecotypes were grown in trays, each containing 8 plants perplot, in a greenhouse with controlled temperature conditions for about12 weeks. Plants were irrigated with different nitrogen concentration asdescribed above depending on the treatment applied. During this time,data was collected documented and analyzed. Most of chosen parameterswere analyzed by digital imaging.

Digital Imaging—Greenhouse Assay

An image acquisition system, which consists of a digital reflex camera(Canon EOS 400D) attached with a 55 mm focal length lens (Canon EF-Sseries) placed in a custom made Aluminum mount, was used for capturingimages of plants planted in containers within an environmentalcontrolled greenhouse. The image capturing process is repeated every 2-3days starting at day 9-12 till day 16-19 (respectively) fromtransplanting.

An image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels(3888×2592 pixels) and stored in a low compression JPEG (JointPhotographic Experts Group standard) format. Next, image processingoutput data was saved to text files and analyzed using the JMPstatistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated,including leaf number, leaf blade area, Rosette diameter and area.

Relative Growth Area Rate:

The relative growth rate of the rosette and the leaves was calculatedaccording to Formulas V and VI:

Relative growth rate rosette area=Regression coefficient of rosette areaalong time course  Formula V

Relative growth rate of leaves area=Regression coefficient of leavesarea along time course  Formula VI

Seed yield and 1000 seeds weight—At the end of the experiment all seedsfrom all plots were collected and weighed in order to measure seed yieldper plant in terms of total seed weight per plant (gr). For thecalculation of 1000 seed weight, an average weight of 0.02 grams wasmeasured from each sample, the seeds were scattered on a glass tray anda picture was taken. Using the digital analysis, the number of seeds ineach sample was calculated.

Dry weight and seed yield—At the end of the experiment, plant wereharvested and left to dry at 30° C. in a drying chamber. The biomass wasseparated from the seeds, weighed and divided by the number of plants.Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 30° C. in a drying chamber.

Harvest index—The harvest index was calculated using Formula IV asdescribed above.

T₅₀ days to flowering—Each of the repeats was monitored for floweringdate. Days of flowering was calculated from sowing date till 50% of theplots flowered.

Plant nitrogen level—The chlorophyll content of leaves is a goodindicator of the nitrogen plant status since the degree of leafgreenness is highly correlated to this parameter. Chlorophyll contentwas determined using a Minolta SPAD 502 chlorophyll meter andmeasurement was performed at time of flowering. SPAD meter readings weredone on young fully developed leaves. Three measurements per leaf weretaken per plot. Based on this measurement, parameters such as the ratiobetween seed yield per nitrogen unit [seed yield/N level=seed yield perplant [gr]/SPAD unit], plant DW per nitrogen unit [DW/N level=plantbiomass per plant [g]/SPAD unit], and nitrogen level per gram of biomass[N level/DW=SPAD unit/plant biomass per plant (gr)] were calculated.

Percent of seed yield reduction—measures the amount of seeds obtained inplants when grown under nitrogen-limiting conditions compared to seedyield produced at normal nitrogen levels expressed in percentages (%).

Experimental Results

10 different Arabidopsis accessions (ecotypes) were grown andcharacterized for 37 parameters as described above. The average for eachof the measured parameters was calculated using the JMP software andvalues are summarized in Table 5 below. Subsequent correlation analysisbetween the various transcriptom sets (Table 3) and the measuredparameters was conducted (Table 6 below). Following are the resultsintegrated to the database.

TABLE 5 Measured parameters in Arabidopsis accessions Ecotype\ TreatmentLine-1 Line-2 Line-3 Line-4 Line-5 Line-6 Line-7 Line-8 Line-9 Line-10 N1.5 mM; 0.760 0.709 1.061 1.157 0.996 1.000 0.910 0.942 1.118 0.638Rosette Area at day 8 N 1.5 mM; 1.430 1.325 1.766 1.971 1.754 1.8321.818 1.636 1.996 1.150 Rosette Area at day 10 N 1.5 mM; 3.221 3.0034.497 4.902 4.220 4.238 3.858 3.990 4.738 2.705 Plot Coverage % at day 8N 1.5 mM; 6.058 5.614 7.484 8.351 7.432 7.764 7.702 6.933 8.458 4.871Plot Coverage % at day 10 N 1.5 mM; 6.875 7.313 7.313 7.875 7.938 7.7507.625 7.188 8.625 5.929 Leaf Number at day 10 N 1.5 mM; 0.335 0.2660.374 0.387 0.373 0.370 0.386 0.350 0.379 0.307 Leaf Blade Area at day10 N 1.5 mM; 0.631 0.793 0.502 0.491 0.605 0.720 0.825 0.646 0.668 0.636RGR of Rosette Area at day 3 N 1.5 mM; 15.967 20.968 14.836 24.70823.566 23.698 18.059 19.488 23.568 21.888 t50 Flowering [day] N 1.5 mM;0.164 0.124 0.082 0.113 0.184 0.124 0.134 0.106 0.148 0.171 Dry Weight[gr/plant] N 1.5 mM; 0.032 0.025 0.023 0.010 0.006 0.009 0.032 0.0190.012 0.014 Seed Yield [gr/plant] N 1.5 mM; 0.192 0.203 0.295 0.0850.031 0.071 0.241 0.179 0.081 0.079 Harvest Index N 1.5 mM; 0.016 0.0160.018 0.014 0.018 0.022 0.015 0.014 0.022 0.019 1000 Seeds weight[gr] N1.5 mM; 0.022 0.019 0.014 0.005 0.003 0.005 0.018 0.013 0.007 0.012 seedyield/ rosette area day at day 10 N 1.5 mM; 0.095 0.095 0.063 0.0260.015 0.024 0.084 0.059 0.034 0.044 seed yield/leaf blade N 1.5 mM;72.559 84.701 78.784 87.996 91.820 92.622 76.710 81.938 91.301 85.757 %Seed yield reduction compared to 6 mM N 1.5 mM; 60.746 76.706 78.56078.140 62.972 78.641 73.192 83.068 77.190 70.120 % Biomass reductioncompared to 6 mM N 1.5 mM; 45.590 42.108 28.151 53.111 67.000 Spad/FW N1.5 mM; 167.300 241.061 157.823 194.977 169.343 SPAD/DW N 1.5 mM; 0.0060.004 0.006 0.005 0.006 DW/SPAD N 1.5 mM; 0.001 0.000 0.000 0.001 0.000seed yield/spad N 6 mM; 0.759 0.857 1.477 1.278 1.224 1.095 1.236 1.0941.410 0.891 Rosette Area at day 8 N 6 mM; 1.406 1.570 2.673 2.418 2.2072.142 2.474 1.965 2.721 1.642 Rosette Area at day 10 N 6 mM; 3.216 3.6316.259 5.413 5.187 4.641 5.236 4.634 5.974 3.774 Plot Coverage % at day 8N 6 mM; 5.957 6.654 11.324 10.244 9.352 9.076 10.485 8.327 11.528 6.958Plot Coverage % at day 10 N 6 mM; 6.250 7.313 8.063 8.750 8.063 8.7508.375 7.125 9.438 6.313 Leaf Number at day 10 N 6 mM; 0.342 0.315 0.5230.449 0.430 0.430 0.497 0.428 0.509 0.405 Leaf Blade Area at day 10 N 6mM; 0.689 1.024 0.614 0.601 0.477 0.651 0.676 0.584 0.613 0.515 RGR ofRosette Area at day 3 N 6 mM; t50 16.371 20.500 14.635 24.000 23.37823.595 15.033 19.750 22.887 18.804 Flowering [day] N 6 mM; 0.419 0.5310.382 0.518 0.496 0.579 0.501 0.628 0.649 0.573 Dry Weight [gr/plant] N6 mM; 0.116 0.165 0.108 0.082 0.068 0.119 0.139 0.107 0.138 0.095 SeedYield [gr/plant] N 6 mM; 0.280 0.309 0.284 0.158 0.136 0.206 0.276 0.1710.212 0.166 Harvest Index N 6 mM; 0.015 0.017 0.018 0.012 0.016 0.0160.015 0.014 0.017 0.016 1000 Seeds weight[gr] N 6 mM; 0.082 0.106 0.0410.034 0.031 0.056 0.057 0.055 0.051 0.058 seed yield/ rosette area dayat day 10 N 6 mM; 0.339 0.526 0.207 0.183 0.158 0.277 0.281 0.252 0.2710.235 seed yield/leaf blade N 6 mM; 22.489 28.268 17.641 33.323 39.003Spad/FW N 6 mM; 0.019 0.018 0.028 0.015 0.015 DW/SPAD (biomass/N unit) N6 mM; 53.705 54.625 35.548 66.479 68.054 spad/DW (gN/g plant) N 6 mM;0.004 0.003 0.002 0.005 0.003 Seed yield/N unit Table 5. Provided arethe measured parameters under various treatments in various ecotypes(Arabidopsis accessions).

TABLE 6 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under normal or low nitrogen fertilizationconditions across Arabidopsis accessions Gene P Exp. Corr. Gene Exp.Corr. Name R value set Set ID Name R P value set Set ID LNU512 0.790.0063 B 12 LNU306 0.74 0.0150 B 11 LNU382 0.80 0.0053 B 12 LNU424 0.900.0004 A 27 LNU382 0.71 0.0218 A 5 LNU424 0.88 0.0008 A 8 LNU308 0.790.0065 A 31 LNU424 0.86 0.0012 A 15 LNU308 0.83 0.0052 D 31 LNU424 0.780.0125 D 5 LNU308 0.81 0.0046 C 31 Table 6. “Corr. Set ID” - correlationset ID according to the correlated parameters Table above.

Example 4 Production of Rice Transcriptom Using 44K Rice OligonucleotideMicro-Array

In order to produce differential expression analysis of rice plantssubjected to nitrogen limiting conditions compared to normal(non-limiting) nitrogen conditions, the present inventors have utilizeda Rice oligonucleotide micro-array, produced by Agilent Technologies[Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent(dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotiderepresents about 44,000 rice genes and transcripts.

Experimental Procedures

Rice plants grown under different nitrogen fertilization levelsassessment—Five rice accessions were grown in 3 repetitive plots, eachcontaining 10 plants, at a net house under semi-hydroponics conditions.Briefly, the growing protocol was as follows: Rice seeds were sown intrays filled with a mix of vermiculite and peat in a 1:1 ratio. Constantnitrogen limiting conditions were achieved by irrigating the plants witha solution containing 0.8 mM inorganic nitrogen in the form of KNO₃,supplemented with 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM K₂SO₄ andmicroelements, while normal nitrogen levels were achieved by applying asolution of 8 mM inorganic nitrogen also in the form of KNO₃ with 1 mMKH₂PO₄, 1 mM MgSO₄, and microelements.

Analyzed rice tissues—All 5 selected rice varieties were pooled in 1batch per each treatment. Two tissues [leaves and roots] growing at twodifferent nitrogen fertilization levels, 0.8 mM Nitrogen (nitrogenlimiting conditions) or 8 mM Nitrogen (normal nitrogen conditions) weresampled and RNA was extracted as described above. For convenience, eachmicro-array expression information tissue type has received a Set

ID as summarized in Table 7 below.

TABLE 7 Rice transcriptom expression sets Expression Set Set ID Leavesat 0.8 mM Nitrogen fertilization A Leaves at 8 mM Nitrogen fertilizationB Roots at 0.8 mM Nitrogen fertilization C Roots at 8 mM Nitrogenfertilization D Table 7.

Experimental Results

Gene up-regulation under reduced nitrogen fertilization levels indicatesthe involvement of the genes in NUE improvement.

Example 5 Production of Arabidopsis Transcriptom and High ThroughputCorrelation Analysis of Yield, Biomass and/or Vigor Related ParametersUsing 44K Arabidopsis Full Genome Oligonucleotide Micro-Array

To produce a high throughput correlation analysis comparing betweenplant phenotype and gene expression level, the present inventorsutilized an Arabidopsis thaliana oligonucleotide micro-array, producedby Agilent Technologies [Hypertext Transfer Protocol://World Wide Web(dot) chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879].The array oligonucleotide represents about 44,000 A. thaliana genes andtranscripts designed based on data from the TIGR ATH1 v.5 database andArabidopsis MPSS (University of Delaware) databases. To definecorrelations between the levels of RNA expression and yield, biomasscomponents or vigor related parameters, various plant characteristics of15 different Arabidopsis ecotypes were analyzed. Among them, nineecotypes encompassing the observed variance were selected for RNAexpression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Arabidopsis tissues—Five tissues at different developmentalstages including root, leaf, flower at anthesis, seed at 5 days afterflowering (DAF) and seed at 12 DAF, representing different plantcharacteristics, were sampled and RNA was extracted as described above.Each micro-array expression information tissue type has received a SetID as summarized in Table 8 below.

TABLE 8 Tissues used for Arabidopsis transcriptom expression setsExpression Set Set ID Root A Leaf B Flower C Seed 5 DAF D Seed 12 DAF ETable 8: Provided are the identification (ID) letters of each of theArabidopsis expression sets (A-E). DAF = days after flowering.

Yield components and vigor related parameters assessment—Eight out ofthe nine Arabidopsis ecotypes were used in each of 5 repetitive blocks(named A, B, C, D and E), each containing 20 plants per plot. The plantswere grown in a greenhouse at controlled conditions in 22° C., and theN:P:K fertilizer (20:20:20; weight ratios) [nitrogen (N), phosphorus (P)and potassium (K)] was added. During this time data was collected,documented and analyzed. Additional data was collected through theseedling stage of plants grown in a tissue culture in vertical growntransparent agar plates. Most of chosen parameters were analyzed bydigital imaging.

Digital imaging in tissue culture—A laboratory image acquisition systemwas used for capturing images of plantlets sawn in square agar plates.The image acquisition system consists of a digital reflex camera (CanonEOS 300D) attached to a 55 mm focal length lens (Canon EF-S series),mounted on a reproduction device (Kaiser RS), which included 4 lightunits (4×150 Watts light bulb) and located in a darkroom.

Digital imaging in greenhouse—The image capturing process was repeatedevery 3-4 days starting at day 7 till day 30. The same camera attachedto a 24 mm focal length lens (Canon EF series), placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. The white tubs weresquare shape with measurements of 36×26.2 cm and 7.5 cm deep. During thecapture process, the tubs were placed beneath the iron mount, whileavoiding direct sun light and casting of shadows. This process wasrepeated every 3-4 days for up to 30 days.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P43.0 GHz processor) and a public domain program—ImageJ1.37, Java based image processing program, which was developed at theU.S. National Institutes of Health and is freely available on theinternet at Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/.Images were captured in resolution of 6 Mega Pixels (3072×2048 pixels)and stored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated,including leaf number, area, perimeter, length and width. On day 30, 3-4representative plants were chosen from each plot of blocks A, B and C.The plants were dissected, each leaf was separated and was introducedbetween two glass trays, a photo of each plant was taken and the variousparameters (such as leaf total area, laminar length etc.) werecalculated from the images. The blade circularity was calculated aslaminar width divided by laminar length.

Root analysis—During 17 days, the different ecotypes were grown intransparent agar plates. The plates were photographed every 3 daysstarting at day 7 in the photography room and the roots development wasdocumented (see examples in FIGS. 3A-3F). The growth rate of roots wascalculated according to Formula VII.

Relative growth rate of root coverage=Regression coefficient of rootcoverage along time course.  Formula VII:

Vegetative growth rate analysis—was calculated according to FormulaVIII. The analysis was ended with the appearance of overlapping plants.

Relative vegetative growth rate area=Regression coefficient ofvegetative area along time course.  Formula VIII:

For comparison between ecotypes the calculated rate was normalized usingplant developmental stage as represented by the number of true leaves.In cases where plants with 8 leaves had been sampled twice (for exampleat day 10 and day 13), only the largest sample was chosen and added tothe Anova comparison.

Seeds in siliques analysis—On day 70, 15-17 siliques were collected fromeach plot in blocks D and E. The chosen siliques were light brown colorbut still intact. The siliques were opened in the photography room andthe seeds were scatter on a glass tray, a high resolution digitalpicture was taken for each plot. Using the images the number of seedsper silique was determined.

Seeds average weight—At the end of the experiment all seeds from plotsof blocks A-C were collected. An average weight of 0.02 grams wasmeasured from each sample, the seeds were scattered on a glass tray anda picture was taken. Using the digital analysis, the number of seeds ineach sample was calculated.

Oil percentage in seeds—At the end of the experiment all seeds fromplots of blocks A-C were collected. Columbia seeds from 3 plots weremixed grounded and then mounted onto the extraction chamber. 210 ml ofn-Hexane (Cat No. 080951 Biolab Ltd.) were used as the solvent. Theextraction was performed for 30 hours at medium heat 50° C. Once theextraction has ended the n-Hexane was evaporated using the evaporator at35° C. and vacuum conditions. The process was repeated twice. Theinformation gained from the Soxhlet extractor (Soxhlet, F. Diegewichtsanalytische Bestimmung des Milchfettes, Polytechnisches J.(Dingler's) 1879, 232, 461) was used to create a calibration curve forthe Low Resonance NMR. The content of oil of all seed samples wasdetermined using the Low Resonance NMR (MARAN Ultra-Oxford Instrument)and its MultiQuant software package.

Silique length analysis—On day 50 from sowing, 30 siliques fromdifferent plants in each plot were sampled in block A. The chosensiliques were green-yellow in color and were collected from the bottomparts of a grown plant's stem. A digital photograph was taken todetermine silique's length.

Dry weight and seed yield—On day 80 from sowing, the plants from blocksA-C were harvested and left to dry at 30° C. in a drying chamber. Thebiomass and seed weight of each plot was separated, measured and dividedby the number of plants. Dry weight=total weight of the vegetativeportion above ground (excluding roots) after drying at 30° C. in adrying chamber; Seed yield per plant=total seed weight per plant (gr).

Oil yield—The oil yield was calculated using Formula IX.

Seed Oil yield=Seed yield per plant (gr.)*Oil % in seed.  Formula IX:

Harvest Index (seed)—The harvest index was calculated using Formula IV(described above).

Experimental Results

Nine different Arabidopsis ecotypes were grown and characterized for 18parameters (named as vectors).

TABLE 9 Arabidopsis correlated parameters (vectors) Correlated parameterwith Correlation ID Root length day 13 (cm) 1 Root length day 7 (cm) 2Relative root growth (cm/day) day 13 3 Fresh weight per plant (gr.) atbolting stage 4 Dry matter per plant (gr.) 5 Vegetative growth rate(cm²/day) till 8 true leaves 6 Blade circularity 7 Lamina width (cm) 8Lamina length (cm) 9 Total leaf area per plant (cm) 10 1000 Seed weight(gr.) 11 Oil % per seed 12 Seeds per silique 13 Silique length (cm) 14Seed yield per plant (gr.) 15 Oil yield per plant (mg) 16 Harvest Index17 Leaf width/length 18 Table 9. Provided are the Arabidopsis correlatedparameters (correlation ID Nos. 1-18). Abbreviations: cm =centimeter(s); gr. = gram(s); mg = milligram(s).

The characterized values are summarized in Tables 10 and 11 below.

TABLE 10 Measured parameters in Arabidopsis ecotypes Ecotype/ParameterID 15 16 12 11 5 17 10 13 14 An-1 0.34 118.63 34.42 0.0203 0.64 0.5346.86 45.44 1.06 Col-0 0.44 138.73 31.19 0.0230 1.27 0.35 109.89 53.471.26 Ct-1 0.59 224.06 38.05 0.0252 1.05 0.56 58.36 58.47 1.31 Cvi(N8580) 0.42 116.26 27.76 0.0344 1.28 0.33 56.80 35.27 1.47 Gr-6 0.61218.27 35.49 0.0202 1.69 0.37 114.66 48.56 1.24 Kondara 0.43 142.1132.91 0.0263 1.34 0.32 110.82 37.00 1.09 Ler-1 0.36 114.15 31.56 0.02050.81 0.45 88.49 39.38 1.18 Mt-0 0.62 190.06 30.79 0.0226 1.21 0.51121.79 40.53 1.18 Shakdara 0.55 187.62 34.02 0.0235 1.35 0.41 93.0425.53 1.00 Table 10. Provided are the values of each of the parametersmeasured in Arabidopsis ecotypes: 15 = Seed yield per plant (gr.); 16 =oil yield per plant (mg); 12 = oil % per seed; 11 = 1000 seed weight(gr.); 5 = dry matter per plant (gr.); 17 = harvest index; 10 = totalleaf area per plant (cm); 13 = seeds per silique; 14 = Silique length(cm).

TABLE 11 Additional measured parameters in Arabidopsis ecotypes Ecotype6 3 2 1 4 9 8 18 7 An-1 0.313 0.631 0.937 4.419 1.510 2.767 1.385 0.3530.509 Col-0 0.378 0.664 1.759 8.530 3.607 3.544 1.697 0.288 0.481 Ct-10.484 1.176 0.701 5.621 1.935 3.274 1.460 0.316 0.450 Cvi 0.474 1.0890.728 4.834 2.082 3.785 1.374 0.258 0.370 (N8580) Gr-6 0.425 0.907 0.9915.957 3.556 3.690 1.828 0.356 0.501 Kondara 0.645 0.774 1.163 6.3724.338 4.597 1.650 0.273 0.376 Ler-1 0.430 0.606 1.284 5.649 3.467 3.8771.510 0.305 0.394 Mt-0 0.384 0.701 1.414 7.060 3.479 3.717 1.817 0.3350.491 Shakdara 0.471 0.782 1.251 7.041 3.710 4.149 1.668 0.307 0.409Table 11. Provided are the values of each of the parameters measured inArabidopsis ecotypes: 6 = Vegetative growth rate (cm²/day) until 8 trueleaves; 3 = relative root growth (cm/day) (day 13); 2 = Root length day7 (cm); 1 = Root length day 13 (cm); 4 = fresh weight per plant (gr.) atbolting stage; 9. = Lamima length (cm); 8 = Lamina width (cm); 18 = Leafwidth/length; 7 = Blade circularity.

Table 12 provides the correlation analyses.

TABLE 12 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under normal or low nitrogen fertilizationconditions across Arabidopsis accessions Corr. Gene Exp. Set Gene Exp.Corr. Name R P value set ID Name R P value set Set ID LNU308 0.76 0.0271B 17 LNU306 0.73 0.039 A 1 LNU308 0.83 0.0116 A 17 LNU424 0.84 0.009 B15 LNU504 0.73 0.0397 C 12 LNU424 0.83 0.0114 B 16 LNU504 0.72 0.0454 B9 LNU424 0.86 0.0065 A 1 LNU504 0.86 0.0066 E 15 LNU424 0.72 0.0443 A 2LNU504 0.77 0.0259 E 16 LNU424 0.80 0.0311 D 11 LNU306 0.87 0.0045 C 13Table 12. “Corr. Set ID” - correlation set ID according to thecorrelated parameters Table above.

Example 6 Production of Barley Transcriptom and High ThroughputCorrelation Analysis Using 44K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparingbetween plant phenotype and gene expression level under normalconditions, the present inventors utilized a Barley oligonucleotidemicro-array, produced by Agilent Technologies [Hypertext TransferProtocol://World Wide Web (dot) chem. (dot) agilent (dot)com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotiderepresents about 44,000 Barley genes and transcripts. In order to definecorrelations between the levels of RNA expression and yield or vigorrelated parameters, various plant characteristics of 25 different Barleyaccessions were analyzed. Among them, 13 accessions encompassing theobserved variance were selected for RNA expression analysis. Thecorrelation between the RNA levels and the characterized parameters wasanalyzed using Pearson correlation test [Hypertext TransferProtocol://World Wide Web (dot) davidmlane (dot) com/hyperstat/A34739(dot) html].

Experimental Procedures

Analyzed Barley tissues—Five tissues at different developmental stages[meristem, flower, booting spike, stem and flag leaf], representingdifferent plant characteristics, were sampled and RNA was extracted asdescribed above. Each micro-array expression information tissue type hasreceived a Set ID as summarized in Table 13 below.

TABLE 13 Barley transcriptom expression sets Expression Set Set IDMeristem A Flower B Booting spike C Stem D Flag leaf E Table 13.

Barley yield components and vigor related parameters assessment—25Barley accessions in 4 repetitive blocks (named A, B, C, and D), eachcontaining 4 plants per plot were grown at net house. Plants werephenotyped on a daily basis following the standard descriptor of barley(Table 14, below). Harvest was conducted while 50% of the spikes weredry to avoid spontaneous release of the seeds. Plants were separated tothe vegetative part and spikes, of them, 5 spikes were threshed (grainswere separated from the glumes) for additional grain analysis such assize measurement, grain count per spike and grain yield per spike. Allmaterial was oven dried and the seeds were threshed manually from thespikes prior to measurement of the seed characteristics (weight andsize) using scanning and image analysis. The image analysis systemincluded a personal desktop computer (Intel P4 3.0 GHz processor) and apublic domain program—ImageJ 1.37 (Java based image processing program,which was developed at the U.S. National Institutes of Health and freelyavailable on the internet [Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/]. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

TABLE 14 Barley standard descriptors Trait Parameter Range DescriptionGrowth habit Scoring 1-9 Prostrate (1) or Erect (9) Hairiness of ScoringP (Presence)/ Absence (1) or Presence (2) basal leaves A (Absence) StemScoring 1-5 Green (1), Basal only or pigmentation Half or more (5) Daysto Days Days from sowing to Flowering emergence of awns Plant heightCentimeter Height from ground level (cm) to top of the longest spikeexcluding awns Spikes per Number Terminal Counting plant Spike lengthCentimeter Terminal Counting 5 spikes (cm) per plant Grains per NumberTerminal Counting 5 spikes spike per plant Vegetative dry GramOven-dried for 48 hours at weight 70° C. Spikes dry Gram Oven-dried for48 hours at weight 30° C. Table 14.

Grains per spike—At the end of the experiment (50% of the spikes weredry) all spikes from plots within blocks A-D were collected. The totalnumber of grains from 5 spikes that were manually threshed was counted.The average grain per spike is calculated by dividing the total grainnumber by the number of spikes.

Grain average size (cm)—At the end of the experiment (50% of the spikeswere dry) all spikes from plots within blocks A-D were collected. Thetotal grains from 5 spikes that were manually threshed were scanned andimages were analyzed using the digital imaging system. Grain scanningwas done using Brother scanner (model DCP-135), at the 200 dpiresolution and analyzed with Image J software. The average grain sizewas calculated by dividing the total grain size by the total grainnumber.

Grain average weight (mgr)—At the end of the experiment (50% of thespikes were dry) all spikes from plots within blocks A-D were collected.The total grains from 5 spikes that were manually threshed were countedand weight. The average weight was calculated by dividing the totalweight by the total grain number.

Grain yield per spike (gr)—At the end of the experiment (50% of thespikes were dry) all spikes from plots within blocks A-D were collected.The total grains from 5 spikes that were manually threshed were weight.The grain yield was calculated by dividing the total weight by the spikenumber.

Spike length analysis—At the end of the experiment (50% of the spikeswere dry) all spikes from plots within blocks A-D were collected. Thefive chosen spikes per plant were measured using measuring tapeexcluding the awns.

Spike number analysis—At the end of the experiment (50% of the spikeswere dry) all spikes from plots within blocks A-D were collected. Thespikes per plant were counted.

Growth habit scoring—At the growth stage 10 (booting), each of theplants was scored for its growth habit nature. The scale that was usedwas 1 for prostate nature till 9 for erect.

Hairiness of basal leaves—At the growth stage 5 (leaf sheath stronglyerect; end of tillering), each of the plants was scored for itshairiness nature of the leaf before the last. The scale that was usedwas 1 for prostate nature till 9 for erect.

Plant height—At the harvest stage (50% of spikes were dry) each of theplants was measured for its height using measuring tape. Height wasmeasured from ground level to top of the longest spike excluding awns.

Days to flowering—Each of the plants was monitored for flowering date.Days of flowering was calculated from sowing date till flowering date.

Stem pigmentation—At the growth stage 10 (booting), each of the plantswas scored for its stem color. The scale that was used was 1 for greentill 5 for full purple.

Vegetative dry weight and spike yield—At the end of the experiment (50%of the spikes were dry) all spikes and vegetative material from plotswithin blocks A-D were collected. The biomass and spikes weight of eachplot was separated, measured and divided by the number of plants.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours;

Spike yield per plant=total spike weight per plant (gr) after drying at30° C. in oven for 48 hours.

Harvest Index (for barley)—The harvest index is calculated using FormulaX.

Harvest Index=Average spike dry weight per plant/(Average vegetative dryweight per plant+Average spike dry weight per plant)  Formula X:

TABLE 15 Barley correlated parameters (vectors) Correlation setCorrelation ID Grains per spike (numbers) 1 Grains size (mm2) 2 Grainweight (miligrams) 3 Grain Yield per spike (gr/spike) 4 Spike length(cm) 5 Spikes per plant (numbers) 6 Growth habit (scores 1-9) 7Hairiness of basal leaves (scoring 1-2) 8 Plant height (cm) 9 Days toflowering (days) 10 Stem pigmentation (scoring 1-5) 11 Vegetative dryweight (gram) 12 Harvest Index (ratio) 13 Table 15.

Experimental Results

13 different Barley accessions were grown and characterized for 13parameters as described above. The average for each of the measuredparameter was calculated using the JMP software and values aresummarized in Tables 16 and 17 below. Subsequent correlation analysisbetween the various transcriptom sets (Table 13) and the measuredparameters (Tables 16 and 17), was conducted (Table 18). Follow, resultswere integrated to the database.

TABLE 16 Measured parameters of correlation IDs in Barley accessionsAccession/ Parameter 6 10 3 5 2 1 7 Amatzya 48.85 62.40 35.05 12.04 0.2720.23 2.60 Ashqelon 48.27 64.08 28.06 10.93 0.23 17.98 2.00 Canada park37.42 65.15 28.76 11.83 0.24 17.27 1.92 Havarim stream 61.92 58.92 17.879.90 0.17 17.73 3.17 Jordan est 33.27 63.00 41.22 11.68 0.29 14.47 4.33Klil 41.69 70.54 29.73 11.53 0.28 16.78 2.69 Maale Efraim ND 52.80 25.228.86 0.22 13.47 3.60 Mt Arbel 40.63 60.88 34.99 11.22 0.28 14.07 3.50 MtHarif 62.00 58.10 20.58 11.11 0.19 21.54 3.00 Neomi 49.33 53.00 27.508.58 0.22 12.10 3.67 Neot Kdumim 50.60 60.40 37.13 10.18 0.27 14.36 2.47Oren canyon 43.09 64.58 29.56 10.51 0.27 15.28 3.50 Yeruham 51.40 56.0019.58 9.80 0.18 17.07 3.00 Table 16. Provided are the values of each ofthe parameters measured in Barley accessions according to the followingcorrelation identifications (Correlation Ids): 6 = Spikes per plant; 10= Days to flowering; 3 = Grain weight; 5 = Spike length; 2 = GrainsSize; 1 = Grains per spike; 7 = Growth habit.

TABLE 17 Barley accessions, additional measured parameters Accession/Parameter 8 9 4 11 12 13 Amatzya 1.53 134.27 3.56 1.13 78.87 0.45Ashqelon 1.33 130.50 2.54 2.50 66.14 0.42 Canada park 1.69 138.77 2.581.69 68.49 0.40 Havarim stream 1.08 114.58 1.57 1.75 53.39 0.44 Jordanest 1.42 127.75 3.03 2.33 68.30 0.43 Klil 1.69 129.38 2.52 2.31 74.170.40 Maale Efraim 1.30 103.89 1.55 1.70 35.35 0.52 Mt Arbel 1.19 121.632.62 2.19 58.33 0.48 Mt Harif 1.00 126.80 2.30 2.30 62.23 0.44 Neomi1.17 99.83 1.68 1.83 38.32 0.49 Neot Kdumim 1.60 121.40 2.68 3.07 68.310.45 Oren canyon 1.08 118.42 2.35 1.58 56.15 ND Yeruham 1.17 117.17 1.672.17 42.68 ND Table 17. Provided are the values of each of theparameters measured in Barley accessions according to the followingcorrelation identifications (Correlation Ids): 8 = Hairiness of basalleaves; 9 = Plant height; 4 = Grain yield per spike; 11 = Stempigmentation; 12 = Vegetative dry weight; 13 = Harvest Index.

TABLE 18 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under normal fertilization conditions acrossbarley accessions Gene P Exp. Corr. Gene P Exp. Corr. Name R value SetSet ID Name R value Set Set ID LNU407 0.81 0.0087 C 2 LNU408 0.75 0.0308B 10 LNU407 0.80 0.0032 C 2 LNU436 0.75 0.0311 B 1 LNU407 0.75 0.0078 C3 LNU436 0.74 0.0144 B 1 LNU407 0.75 0.0211 C 3 LNU467 0.76 0.0289 B 1LNU435 0.84 0.0049 C 2 LNU447 0.87 0.0054 B 8 LNU435 0.75 0.0191 C 3LNU447 0.75 0.0119 B 8 LNU435 0.71 0.0138 C 2 LNU297 0.85 0.0072 A 6LNU456 0.71 0.0470 C 6 LNU297 0.75 0.0075 A 6 LNU305 0.87 0.0051 B 10LNU436 0.77 0.0148 A 1 LNU305 0.81 0.0138 B 9 LNU436 0.76 0.0071 A 1LNU305 0.81 0.0048 B 9 LNU448 0.74 0.0348 A 6 LNU305 0.77 0.0242 B 5LNU438 0.85 0.0071 A 6 LNU305 0.76 0.0111 B 5 LNU467 0.77 0.0054 A 6LNU305 0.75 0.0125 B 10 LNU467 0.75 0.0332 A 6 LNU435 0.81 0.0159 B 7LNU447 0.79 0.0106 A 8 LNU435 0.75 0.0119 B 7 LNU447 0.79 0.0036 A 4LNU408 0.79 0.0186 B 12 LNU447 0.77 0.0160 A 4 LNU408 0.79 0.0188 B 4LNU447 0.73 0.0107 A 8 Table 18. “Corr. Set ID” - correlation set IDaccording to the correlated parameters Table above.

Example 7 Production of Sorghum Transcriptom and High ThroughputCorrelation Analysis with Yield, NUE, and ABST Related ParametersMeasured in Fields Using 44K Sorguhm Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis between plantphenotype and gene expression level, the present inventors utilized asorghum oligonucleotide micro-array, produced by Agilent Technologies[Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent(dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotiderepresents about 44,000 sorghum genes and transcripts. In order todefine correlations between the levels of RNA expression with ABST,yield and NUE components or vigor related parameters, various plantcharacteristics of 17 different sorghum hybrids were analyzed. Amongthem, 10 hybrids encompassing the observed variance were selected forRNA expression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Correlation of Sorghum Varieties Across Ecotypes Grown Under LowNitrogen, Regular Growth and Severe Drought Conditions

Experimental Procedures

17 Sorghum varieties were grown in 3 repetitive plots, in field.Briefly, the growing protocol was as follows:

1. Regular Growth Conditions:

sorghum plants were grown in the field using commercial fertilizationand irrigation protocols.

2. Low Nitrogen Fertilization Conditions:

sorghum plants were fertilized with 50% less amount of nitrogen in thefield than the amount of nitrogen applied in the regular growthtreatment. All the fertilizer was applied before flowering. 3. Droughtstress: sorghum seeds were sown in soil and grown under normal conditionuntil around 35 days from sowing, around stage V8 (eight green leavesare fully expanded, booting not started yet). At this point, irrigationwas stopped, and severe drought stress was developed.

Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sample pereach treatment. Plant tissues [Flag leaf, Flower meristem and Flower]growing under low nitrogen, severe drought stress and plants grown undernormal conditions were sampled and RNA was extracted as described above.Each micro-array expression information tissue type has received a SetID as summarized in Table 19 below.

TABLE 19 Sorghum transcriptom expression sets in field experimentsExpression Set Set ID sorghum field/flag leaf/Drought A sorghumfield/flag leaf/Low N B sorghum field/flag leaf/Normal C sorghumfield/flower meristem/Drought D sorghum field/flower meristem/Low N Esorghum field/flower meristem/Normal F sorghum field/flower/Drought Gsorghum field/flower/Low N H sorghum field/flower/Normal J Table 19:Provided are the sorghum transcriptom expression sets. Flag leaf = theleaf below the flower; Flower meristem = Apical meristem followingpanicle initiation; Flower = the flower at the anthesis day.

The following parameters were collected using digital imaging system:

Average grain area (cm²)—At the end of the growing period the grainswere separated from the Plant ‘Head’. A sample of ˜200 grains wereweight, photographed and images were processed using the below describedimage processing system. The grain area was measured from those imagesand was divided by the number of grains.

Average grain length (cm)—At the end of the growing period the grainswere separated from the Plant ‘Head’. A sample of −200 grains wereweighted, photographed and images were processed using the belowdescribed image processing system. The sum of grain lengths (longestaxis) was measured from those images and was divided by the number ofgrains.

Head Average Area (cm²)

At the end of the growing period 5 ‘Heads’ were, photographed and imageswere processed using the below described image processing system. The‘Head’ area was measured from those images and was divided by the numberof ‘Heads’.

Head Average Length (cm)

At the end of the growing period 5 ‘Heads’ were, photographed and imageswere processed using the below described image processing system. The‘Head’ length (longest axis) was measured from those images and wasdivided by the number of ‘Heads’.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels(3888×2592 pixels) and stored in a low compression JPEG (JointPhotographic Experts Group standard) format. Next, image processingoutput data for seed area and seed length was saved to text files andanalyzed using the JMP statistical analysis software (SAS institute).

Additional parameters were collected either by sampling 5 plants perplot or by measuring the parameter across all the plants within theplot.

Total seed weight per head (gr.)—At the end of the experiment (plant‘Heads’) heads from plots within blocks A-C were collected. 5 heads wereseparately threshed and grains were weighted, all additional heads werethreshed together and weighted as well. The average grain weight perhead was calculated by dividing the total grain weight by number oftotal heads per plot (based on plot). In case of 5 heads, the totalgrains weight of 5 heads was divided by 5.

FW head per plant gram—At the end of the experiment (when heads wereharvested) total and 5 selected heads per plots within blocks A-C werecollected separately. The heads (total and 5) were weighted (gr.)separately and the average fresh weight per plant was calculated fortotal (FW Head/Plant gr. based on plot) and for 5 (FW Head/Plant gr.based on 5 plants).

Plant height—Plants were characterized for height during growing periodat 5 time points. In each measure, plants were measured for their heightusing a measuring tape. Height was measured from ground level to top ofthe longest leaf.

Plant leaf number—Plants were characterized for leaf number duringgrowing period at 5 time points. In each measure, plants were measuredfor their leaf number by counting all the leaves of 3 selected plantsper plot.

Relative Growth Rate was calculated using Formulas XI and XII.

Relative growth rate of plant height=Regression coefficient of plantheight along time course.  Formula XI

Relative growth rate of plant leaf number=Regression coefficient ofplant leaf number along time course.  Formula XII

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed 64 days post sowing.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot.

Vegetative dry weight and heads—At the end of the experiment (whenInflorescence were dry) all Inflorescence and vegetative material fromplots within blocks A-C were collected. The biomass and Heads weight ofeach plot was separated, measured and divided by the number of Heads.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours;

Harvest Index (HI) (Sorghum)—The harvest index was calculated usingFormula XIII

Harvest Index=Average grain dry weight per Head/(Average vegetative dryweight per Head+Average Head dry weight)  Formula XIII:

FW Heads/(FW Heads+FW Plants)—The total fresh weight of heads and theirrespective plant biomass was measured at the harvest day. The headsweight was divided by the sum of weights of heads and plants.

Experimental Results

17 different sorghum hybrids were grown and characterized for differentparameters (Table 20). The average for each of the measured parameterwas calculated using the JMP software (Tables 21-25) and a subsequentcorrelation analysis was performed (Table 26). Results were thenintegrated to the database.

TABLE 20 Sorghum correlated parameters (vectors) Correlation setCorrelation ID [Grain yield/SPAD 64 DPS], Low N 1 [Grain yield/SPAD 64DPS], Normal 2 [Grain Yield + plant biomass/SPAD 64 DPS], Low N 3 [GrainYield + plant biomass/SPAD 64 DPS], Normal 4 [Plant biomass (FW)/SPAD 64DPS], Drought 5 [Plant biomass (FW)/SPAD 64 DPS], Low N 6 [Plant biomass(FW)/SPAD 64 DPS], Normal 7 Average Grain Area (cm2), Drought 8 AverageGrain Area (cm2), Low N 9 Average Grain Area (cm2), Normal 10 FinalPlant Height (cm), Drought 11 Final Plant Height (cm), Low N 12 FinalPlant Height (cm), Normal 13 FW—Head/Plant gr. (based on 5 plants), LowN 14 FW—Head/Plant gr. (based on 5 plants), Normal 15 FW—Head/Plant gr.(based on plot), Drought 16 FW—Head/Plant gr. (based on plot), Low N 17FW—Head/Plant gr. (based on plot), Normal 18 FW Heads/(FW Heads + FWPlants)(all plot), Drought 19 FW Heads/(FW Heads + FW Plants)(all plot),Low N 20 FW Heads/(FW Heads + FW Plants)(all plot), Normal 21 FW/Plantgr. (based on plot), Drought 22 FW/Plant gr. (based on plot), Low N 23FW/Plant gr. (based on plot), Normal 24 Head Average Area (cm2), Drought25 Head Average Area (cm2), Low N 26 Head Average Area (cm2), Normal 27Head Average Length (cm), Drought 28 Head Average Length (cm), Low N 29Head Average Length (cm), Normal 30 Head Average Perimeter (cm), Drought31 Head Average Perimeter (cm), Low N 32 Head Average Perimeter (cm),Normal 33 Head Average Width (cm), Drought 34 Head Average Width (cm),Low N 35 Head Average Width (cm), Normal 36 Leaf SPAD 64 DPS (Days PostSowing), Drought 37 Leaf SPAD 64 DPS (Days Post Sowing), Low N 38 LeafSPAD 64 DPS (Days Post Sowing), Normal 39 Lower Ratio Average GrainArea, Low N 40 Lower Ratio Average Grain Area, Normal 41 Lower RatioAverage Grain Length, Low N 42 Lower Ratio Average Grain Length, Normal43 Lower Ratio Average Grain Perimeter, Low N 44 Lower Ratio AverageGrain Perimeter, Normal 45 Lower Ratio Average Grain Width, Low N 46Lower Ratio Average Grain Width, Normal 47 Total grain weight/Head(based on plot) gr., Low N 48 Total grain weight/Head gr. (based on 5heads), Low N 49 Total grain weight/Head gr. (based on 5 heads), Normal50 Total grain weight/Head gr. (based on plot), Normal 51 Total grainweight/Head gr., (based on plot), Drought 52 Upper Ratio Average GrainArea, Drought 53 Upper Ratio Average Grain Area, Low N 54 Upper RatioAverage Grain Area, Normal 55 Table 20. Provided are the Sorghumcorrelated parameters (vectors). “gr.” = grams; “SPAD” = chlorophylllevels; “FW” = Plant Fresh weight; “DW” = Plant Dry weight; “normal” =standard growth conditions; “DPS” = days post-sowing; “Low N” = LowNitrogen.

TABLE 21 Measured parameters in Sorghum accessions under normalconditions Correlation ID Seed ID 2 4 7 10 13 15 18 21 24 27 30 20 3.784.5 0.724 0.105 95.2 406 175 0.51 163 120 25.6 21 7.74 8.17 0.433 0.11279.2 518 223 0.51 213 168 26.8 22 7.01 7.87 0.858 0.131 198 148 56.40.115 335 85.1 21 24 10.1 10.7 0.583 0.129 234 423 112 0.263 313 15726.8 25 7.65 8.34 0.693 0.139 189 92 67.3 0.12 462 104 23.1 26 3.34 4.41.05 0.141 195 101 66.9 0.177 318 102 21.8 27 3.05 3.73 0.687 0.11 117424 126 0.459 151 169 31.3 28 3.9 4.83 0.929 0.113 92.8 386 108 0.432138 109 23.2 29 2.83 3.67 0.841 0.102 113 410 124 0.425 168 135 25.7 302.18 2.89 0.716 0.118 97.5 329 103 0.442 129 169 28.8 31 2.19 2.91 0.7210.121 98 391 82.3 0.458 97.6 156 28.1 32 2.41 3.12 0.705 0.111 100 43677.6 0.447 99.3 112 23 33 3.58 4.75 1.17 0.117 106 430 91.2 0.447 112155 28.1 34 2.9 3.69 0.792 0.108 151 441 150 0.513 157 172 30 35 3 3.850.849 0.105 117 416 109 0.46 131 169 30.5 36 4.85 5.83 0.984 0.11 124430 108 0.442 136 163 27.2 37 0.105 126 428 131 0.386 209 170 29.3 Table21: Provided are the values of each of the parameters (as describedabove) measured in Sorghum accessions (Seed ID) under normal conditions.Growth conditions are specified in the experimental procedure section.

TABLE 22 Additional measured parameters in Sorghum accessions undernormal growth conditions Corr. ID Seed ID 33 36 39 41 43 45 47 50 51 5520 61.2 5.97 43 0.825 0.914 0.914 0.908 41.4 31.1 1.22 21 67.9 7.92 00.74 0.884 0.869 0.833 46.3 26.4 1.3 22 56.3 4.87 43.3 0.778 0.921 0.9130.85 28.4 18.7 1.13 24 65.4 7.43 44.7 0.802 0.908 0.948 0.874 70.4 38.41.14 25 67.5 5.58 45.8 0.697 0.89 0.902 0.788 32.1 26.7 1.16 26 67.55.88 41.6 0.699 0.877 0.915 0.799 49.2 28.8 1.15 27 74.4 6.78 45.2 0.8270.913 0.913 0.904 63.5 47.7 1.19 28 56.2 5.99 45.1 0.805 0.903 0.910.893 44.5 31 1.23 29 61.6 6.62 43 0.841 0.92 0.918 0.915 56.6 40 1.2530 71.4 7.42 45.6 0.788 0.923 0.93 0.854 60 38.4 1.24 31 68.6 6.98 44.80.765 0.893 0.911 0.863 45.5 32.1 1.32 32 56.4 6.19 45.3 0.803 0.9130.916 0.885 58.2 32.7 1.22 33 67.8 7.02 46.5 0.806 0.907 0.904 0.89870.6 32.8 1.18 34 71.5 7.18 44 0.821 0.911 0.912 0.905 70.1 51.5 1.18 3578.9 7 45.1 0.814 0.904 0.905 0.91 54 35.7 1.22 36 67 7.39 45.1 0.8180.903 0.909 0.902 59.9 38.3 1.25 37 74.1 7.35 43.1 0.817 0.913 0.9050.899 52.6 42.4 1.22 Table 22: Provided are the values of each of theparameters (as described above) measured in Sorghum accessions (Seed ID)under normal conditions. Growth conditions are specified in theexperimental procedure section.

TABLE 23 Measured parameters in Sorghum accessions under Low nitrogenconditions Seed Corr. ID ID 1 3 6 9 12 14 17 20 23 26 29 20 0.677 6.025.34 0.105 104 388 215 0.505 205 96.2 23.2 21 0.784 5.91 5.12 0.111 80.9429 205 0.506 200 215 25.6 22 0.458 8.5 8.04 0.136 205 298 73.5 0.166341 98.6 20.9 24 0.871 6.75 5.88 0.121 125 280 123 0.391 241 183 28.4 250.584 13.1 12.5 0.141 225 208 153 0.21 538 120 24.3 26 0.557 9.57 9.020.134 208 304 93.2 0.192 359 110 22.6 27 1.17 4.67 3.5 0.119 121 436 1340.476 149 172 32.1 28 0.634 3.61 2.98 0.117 100 376 77.4 0.375 129 84.820.4 29 1.31 5.89 4.58 0.116 121 475 130 0.42 179 156 26.7 30 0.862 3.772.91 0.129 94.5 438 99.8 0.441 124 137 26.3 31 0.735 3.26 2.53 0.131 110383 76.9 0.429 101 138 25.4 32 0.607 3.61 3 0.12 115 375 84.2 0.387 13296.5 23.1 33 0.648 3.24 2.59 0.116 105 425 92.2 0.438 118 158 27.9 341.14 5.1 3.96 0.115 174 434 139 0.439 177 164 28.9 35 0.87 4.25 3.380.107 116 409 113 0.442 144 138 27.6 36 0.91 3.81 2.9 0.121 139 378 95.50.43 127 135 25.5 37 0.894 4.76 3.86 0.109 144 432 129 0.417 180 16630.3 Table 23: Provided are the values of each of the parameters (asdescribed above) measured in Sorghum accessions (Seed ID) under lownitrogen conditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 24 Additional measured parameters in Sorghum accessions under lownitrogen growth conditions Seed Corr. ID ID 32 35 38 40 42 44 46 48 4954 20 56.3 5.26 38.3 0.815 0.91 0.901 0.901 25.9 50.3 1.18 21 79.2 10.439 0.77 0.9 0.884 0.852 30.6 50.9 1.31 22 53.2 5.93 42.3 0.81 0.9210.915 0.893 19.4 36.1 1.11 24 76.2 8.25 40.9 0.793 0.898 0.897 0.88 35.673.1 1.21 25 67.3 6.19 43.1 0.78 0.908 0.919 0.863 25.2 37.9 1.19 2659.5 6.12 39.9 0.799 0.926 0.918 0.871 22.2 36.4 1.18 27 79.3 6.8 42.70.834 0.918 0.916 0.91 50 71.7 1.16 28 51.5 5.25 43.3 0.788 0.89 0.8910.888 27.5 35 1.23 29 69.9 7.52 39 0.806 0.901 0.898 0.899 51.1 76.71.17 30 66.2 6.59 42.7 0.772 0.909 0.907 0.857 36.8 57.6 1.22 31 67.46.85 40.1 0.741 0.886 0.895 0.842 29.4 42.9 1.24 32 57.9 5.32 44 0.8040.897 0.903 0.897 26.7 36.5 1.19 33 70.6 7.25 45.4 0.788 0.894 0.8960.887 29.4 68.6 1.23 34 73.8 7.19 44.8 0.823 0.911 0.914 0.908 51.1 71.81.16 35 66.9 6.27 42.6 0.801 0.888 0.894 0.899 37 49.3 1.34 36 65.4 6.5743.8 0.809 0.892 0.896 0.902 39.9 43.9 1.21 37 76 6.82 46.7 0.807 0.9010.897 0.897 41.8 52.1 1.21 Table 24: Provided are the values of each ofthe parameters (as described above) measured in Sorghum accessions (SeedID) under low nitrogen conditions. Growth conditions are specified inthe experimental procedure section.

TABLE 25 Measured parameters in Sorghum accessions under droughtconditions Correlation ID Seed ID 5 8 11 16 19 22 25 28 31 34 37 52 5320 5.13 0.10 89 155 0.42 208 83 21.6 52.8 4.83 40.6 22.1 1.31 21 3.380.12 76 122 0.47 138 108 21.9 64.5 6.31 40.9 16.8 1.19 22 5.67 0.11 92131 0.42 255 89 21.6 56.6 5.16 45 9.19 1.29 24 9.51 0.09 94 241 0.37 402136 22.0 64.4 7.78 42.3 104 1.46 25 5.16 0.09 151 69 0.23 234 91 21.053.2 5.28 45.2 3.24 1.21 26 9.66 0.11 111 186 0.31 392 124 28.6 71.75.49 40.6 22 1.21 27 1.99 99 62 0.41 89 86 21.3 55.6 5.04 44.8 9.97 281.12 84 39 0.44 51 85 20.8 53.0 5.07 45.1 18.6 29 2.14 99 59 0.40 87 11324.7 69.8 5.77 40.6 29.3 30 2.65 92 76 0.44 120 101 24.3 65.1 5.37 45.410.5 31 0.87 82 34 0.47 37 80 21.9 55.3 4.66 42.6 14.8 32 1.09 99 420.47 48 127 25.0 69.1 6.35 44.2 12.9 33 0.99 87 42 0.48 44 86 19.5 53.35.58 44.6 18.2 34 5.46 100 132 0.35 232 92 20.4 56.3 5.76 42.4 11.6 352.68 83 61 0.35 116 78 16.8 49.1 5.86 43.2 18.6 36 3.05 84 44 0.23 12377 18.9 51.9 5.1 40.3 16.4 37 8.40 92 185 0.33 342 40.8 Table 25:Provided are the values of each of the parameters (as described above)measured in Sorghum accessions (Seed ID) under drought conditions.Growth conditions are specified in the experimental procedure section.

TABLE 26 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under low nitrogen, normal or drought stressconditions across Sorghum accessions Gene P Corr. Exp. Gene P Corr. ExpName R value ID set ID Name R value ID set ID LNU316 0.7795 0.0079 10 FLNU316 0.9108 0.0002 48 B LNU316 0.7755 0.0084 29 H LNU401 0.9089 0.00032 F LNU316 0.7699 0.0092 9 E LNU477 0.9069 0.0003 6 E LNU316 0.76750.0095 44 H LNU421 0.9065 0.0003 30 C LNU316 0.7963 0.0058 22 G LNU4010.9035 0.0003 4 F LNU316 0.7351 0.0154 30 J LNU473 0.9005 0.0004 23 ELNU316 0.7309 0.0163 42 H LNU421 0.8970 0.0004 13 F LNU319 0.7256 0.017520 H LNU480 0.8965 0.0004 22 D LNU319 0.7173 0.0195 35 H LNU480 0.89610.0004 5 D LNU324 0.8312 0.0029 6 E LNU439 0.8943 0.0011 2 C LNU3240.8294 0.0030 3 E LNU316 0.8906 0.0005 12 B LNU324 0.8068 0.0048 5 DLNU481 0.8898 0.0013 2 C LNU324 0.7641 0.0101 13 F LNU401 0.8894 0.000618 F LNU324 0.8216 0.0035 16 D LNU473 0.8853 0.0007 6 E LNU324 0.75120.0123 20 E LNU421 0.8825 0.0007 27 C LNU324 0.8178 0.0038 22 D LNU4210.8823 0.0007 33 C LNU324 0.8534 0.0017 23 E LNU314 0.8822 0.0007 51 FLNU324 0.7055 0.0226 51 F LNU401 0.8811 0.0008 24 F LNU346 0.7275 0.01716 B LNU393 0.8811 0.0008 24 F LNU346 0.7022 0.0236 3 B LNU477 0.87850.0008 23 E LNU346 0.7742 0.0086 5 D LNU477 0.8784 0.0008 3 E LNU3460.8008 0.0054 20 B LNU431 0.8763 0.0009 1 B LNU346 0.7521 0.0121 13 FLNU465 0.8756 0.0009 13 J LNU346 0.8440 0.0021 16 D LNU401 0.8713 0.001015 F LNU346 0.7834 0.0073 17 B LNU473 0.8666 0.0012 17 E LNU346 0.70580.0226 14 E LNU324 0.8647 0.0012 17 E LNU346 0.7361 0.0152 20 H LNU4770.8608 0.0014 17 E LNU346 0.7797 0.0078 22 D LNU439 0.8595 0.0014 15 CLNU346 0.7049 0.0228 23 E LNU481 0.8579 0.0015 13 J LNU346 0.7254 0.017626 E LNU481 0.8566 0.0032 4 C LNU346 0.7488 0.0127 51 F LNU393 0.85570.0016 35 B LNU346 0.7046 0.0229 32 E LNU479 0.8544 0.0016 51 F LNU3470.7303 0.0165 11 A LNU303 0.8543 0.0016 12 B LNU347 0.8358 0.0026 30 CLNU313 0.8538 0.0017 1 E LNU347 0.8189 0.0038 10 F LNU316 0.8484 0.00191 B LNU347 0.8401 0.0023 12 B LNU393 0.8335 0.0027 1 E LNU347 0.76130.0105 9 E LNU421 0.8292 0.0030 1 B LNU347 0.7598 0.0108 33 C LNU4200.8292 0.0030 1 H LNU347 0.7113 0.0211 50 C LNU292 0.8235 0.0034 1 ELNU347 0.7336 0.0157 51 C LNU421 0.8106 0.0044 1 E LNU377 0.7291 0.016713 J LNU439 0.8095 0.0045 1 B LNU379 0.7141 0.0204 9 E LNU292 0.84780.0019 48 E LNU381 0.7525 0.0120 1 B LNU292 0.7539 0.0118 14 E LNU3810.7736 0.0144 2 C LNU303 0.7108 0.0212 5 G LNU381 0.7785 0.0135 4 CLNU303 0.7450 0.0134 16 G LNU381 0.7712 0.0090 12 B LNU303 0.7079 0.022022 G LNU381 0.7720 0.0089 37 D LNU303 0.8385 0.0024 35 H LNU381 0.77000.0092 48 B LNU303 0.8195 0.0037 26 H LNU381 0.7079 0.0220 55 F LNU3030.7418 0.0141 54 H LNU387 0.7569 0.0113 6 E LNU303 0.7122 0.0208 32 HLNU387 0.7037 0.0344 2 C LNU303 0.7290 0.0168 1 B LNU387 0.7709 0.0150 4C LNU303 0.7531 0.0119 48 B LNU387 0.7072 0.0222 3 E LNU303 0.72990.0166 5 D LNU387 0.7881 0.0068 17 E LNU303 0.7970 0.0058 16 D LNU3870.7259 0.0175 54 B LNU303 0.7340 0.0157 22 D LNU387 0.7611 0.0106 18 FLNU303 0.8268 0.0032 20 E LNU387 0.7209 0.0186 24 F LNU303 0.7030 0.02333 E LNU387 0.7100 0.0214 20 E LNU303 0.7765 0.0082 17 E LNU387 0.70370.0231 21 F LNU313 0.7466 0.0131 1 H LNU393 0.7575 0.0112 1 H LNU3130.7972 0.0057 48 E LNU393 0.8155 0.0040 49 E LNU313 0.7315 0.0162 15 JLNU393 0.7837 0.0073 26 B LNU314 0.7785 0.0080 1 E LNU393 0.7392 0.014614 B LNU314 0.7696 0.0092 1 H LNU393 0.7501 0.0125 42 H LNU314 0.83180.0028 48 E LNU393 0.7266 0.0173 14 E LNU314 0.7249 0.0177 11 D LNU3930.7456 0.0133 48 E LNU314 0.7899 0.0066 29 E LNU393 0.7301 0.0165 49 HLNU314 0.7849 0.0072 30 J LNU393 0.7909 0.0064 18 F LNU314 0.7197 0.018942 E LNU393 0.7242 0.0179 32 E LNU314 0.7117 0.0210 48 H LNU393 0.71820.0193 44 H LNU314 0.7235 0.0180 51 J LNU393 0.7180 0.0194 30 C LNU3160.7780 0.0081 5 G LNU434 0.8400 0.0046 2 C LNU434 0.8500 0.0040 4 CTable 26: “Corr. Set ID”—correlation set ID according to the correlatedparameters Table above.

Example 8 Production of Sorghum Transcriptom and High ThroughputCorrelation Analysis with Yield, NUE, and ABST Related ParametersMeasured in Semi-Hydroponics Conditions Using 44K SorguhmOligonucleotide Micro-Arrays

Sorghum vigor related parameters under low nitrogen, 100 mM NaCl, lowtemperature (10±2° c.) and normal growth conditions—Ten Sorghum hybridswere grown in 3 repetitive plots, each containing 17 plants, at a nethouse under semi-hydroponics conditions. Briefly, the growing protocolwas as follows: Sorghum seeds were sown in trays filled with a mix ofvermiculite and peat in a 1:1 ratio. Following germination, the trayswere transferred to the high salinity solution (100 mM NaCl in additionto the Full Hoagland solution), low temperature (10±2° C. in thepresence of Full Hoagland solution), low nitrogen solution (the amountof total nitrogen was reduced in 90% from the full Hoagland solution(i.e., to a final concentration of 10% from full Hoagland solution,final amount of 1.2 mM N) or at Normal growth solution (Full Hoaglandcontaining 16 mM N solution, at 28±2° C.). Plants were grown at 28±2° C.

Full Hoagland solution consists of: KNO₃-0.808 grams/liter, MgSO₄—0.12grams/liter, KH₂PO₄—0.172 grams/liter and 0.01% (volume/volume) of‘Super coratin’ micro elements (Iron-EDDHA[ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]—40.5 grams/liter;Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1grams/liter), solution's pH should be 6.5-6.8].

Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sampledper each treatment. Three tissues [leaves, meristems and roots] growingat 100 mM NaCl, low temperature (10±2° C.), low Nitrogen (1.2 mM N) orunder Normal conditions were sampled and RNA was extracted as describedabove. Each micro-array expression information tissue type has receiveda Set ID as summarized in Table 27 below.

TABLE 27 Sorghum transcriptom expression sets under semi hydroponicsconditions Expression set Set Id Sorghum roots under Low Nitrogen ASorghum leaves under Low Nitrogen B Sorghum meristems under Low NitrogenC Sorghum roots under Normal Growth D Sorghum leaves under Normal GrowthE Sorghum meristems under Normal Growth F Sorghum roots under 100 mMNaCl G Sorghum leaves under 100 mM NaCl H Sorghum meristems under 100 mMNaCl I Sorghum roots under cold J Sorghum leaves under cold K Sorghummeristems under cold L Table 27: Provided are the Sorghum transcriptomexpression sets. Cold conditions = 10 ± 2° C.; NaCl = 100 mM NaCl; lownitrogen = 1.2 mM Nitrogen; Normal conditions = 16 mM Nitrogen.

Experimental Results

10 different Sorghum hybrids were grown and characterized for variousbiomass and nitrogen use efficiency (NUE) parameters as described inTable 28, below. The average for each of the measured parameter wascalculated using the JMP software and values are summarized in Table28-32 below. Subsequent correlation analysis was performed (Table 33).Results were then integrated to the database.

TABLE 28 Sorghum correlated parameters (vectors) Correlation Correlationset ID DW Root/Plant—100 mM NaCl 1 DW Root/Plant—Cold 2 DWRoot/Plant—Low Nitrogen 3 DW Root/Plant—Normal 4 DW Shoot/Plant—100 mMNaCl 5 DW Shoot/Plant—Cold 6 DW Shoot/Plant—Low Nitrogen 7 DWShoot/Plant—Normal 8 Leaf Number TP1—100 mM NaCl 9 Leaf Number TP1—Cold10 Leaf Number TP1—Low Nitrogen 11 Leaf Number TP1—Normal 12 Leaf NumberTP2—100 mM NaCl 13 Leaf Number TP2—Cold 14 Leaf Number TP2—Low Nitrogen15 Leaf Number TP2—Normal 16 Leaf Number TP3—100 mM NaCl 17 Leaf NumberTP3—Cold 18 Leaf Number TP3—Low Nitrogen 19 Leaf Number TP3—Normal 20Shoot/Root—Normal 21 NUE per roots—Normal 22 NUE per shoots—Normal 23NUE per total biomass—Normal 24 NUE per roots biomass—Low N 25 NUE pershoots biomass—Low N 26 NUE per total biomass—Low N 27 Percent ofreduction of root biomass 28 compared to normal—Low N Percent ofreduction of shoot biomass 29 compared to normal—Low N Percent ofreduction of total biomass 30 compared to normal—Low N Plant HeightTP1—100 mM NaCl 31 Plant Height TP1—Cold 32 Plant Height TP1—Low N 33Plant Height TP1—Normal 34 Plant Height TP2—100 mM NaCl 35 Plant HeightTP2—Cold 36 Plant Height TP2—Low N 37 Plant Height TP2—Normal 38 PlantHeight TP3—100 mM NaCl 39 Plant Height TP3—Low N 40 RGR Leaf Num Normal41 Root Biomass DW [gr.]/SPAD—100 mM NaCl 42 Root Biomass DW[gr.]/SPAD—Cold 43 Root Biomass DW [gr.]/SPAD—Low N 44 Root Biomass DW[gr.]/SPAD—Normal 45 Shoot Biomass DW [gr.]/SPAD—100 mM NaCl 46 ShootBiomass DW [gr.]/SPAD—Cold 47 Shoot Biomass DW [gr.]/SPAD—Low N 48 ShootBiomass DW [gr.]/SPAD—Normal 49 Shoot/Root—Low N 50 SPAD—100 mM NaCl 51SPAD—Cold 52 SPAD—Low Nitrogen 53 SPAD—Normal 54 SPAD 100—mM NaCl 55Total Biomass DW [gr.]/SPAD—100 mM NaCl 56 Total Biomass DW[gr.]/SPAD—Cold 57 Total Biomass DW [gr.]/SPAD—Low N 58 Total Biomass DW[gr.]/SPAD—Normal 59 Table 28: Provided are the Sorghum correlatedparameters. Cold conditions = 10 ± 2° C.; NaCl = 100 mM NaCl; Lownitrogen = 1.2 mM Nitrogen; Normal conditions = 16 mM Nitrogen *TP-1-2-3 refers to time points 1, 2 and 3.

TABLE 29 Sorghum accessions, measured parameters under low nitrogengrowth conditions Corr. Seed ID ID 20 22 26 27 28 29 30 31 34 37 3 0.040.11 0.20 0.10 0.08 0.09 0.13 0.09 0.09 0.09 7 0.08 0.19 0.33 0.16 0.160.16 0.26 0.20 0.13 0.18 11 3.0 3.1 3.9 3.5 3.2 3.1 3.1 3.3 3.1 3.1 154.0 4.6 5.0 4.7 4.6 4.7 5.0 4.9 4.7 4.6 19 3.9 4.3 4.7 4.2 4.3 4.6 4.64.7 4.0 4.1 27 27.5 64.1 115.0 58.0 52.2 35.1 84.6 63.7 47.0 60.0 50 1.91.7 1.7 1.6 2.1 1.8 2.1 2.1 1.5 2.0 25 9.7 23.5 43.9 22.6 16.9 12.4 28.220.5 18.8 20.1 26 17.9 40.6 71.4 35.4 35.3 22.7 56.4 43.2 28.3 39.9 2884.5 81.0 117.0 101.0 72.5 71.8 93.5 76.1 86.8 80.5 29 81.6 79.2 105.0103.0 83.7 83.2 108.0 81.4 70.3 75.9 30 82.6 79.8 109.0 102.0 79.7 78.8102.0 79.6 76.1 77.4 53 6.89 6.57 6.31 7.45 6.89 5.87 6.15 6.05 7.686.74 33 6.73 9.77 12.70 8.67 9.77 9.23 10.30 10.10 7.93 8.23 37 13.320.6 23.7 18.0 19.3 19.2 21.9 22.1 18.2 21.0 40 22.2 31.1 34.7 30.0 30.829.9 30.9 32.4 29.4 30.7 44 0.002 0.004 0.007 0.003 0.003 0.003 0.0050.003 0.003 0.003 48 0.003 0.007 0.011 0.005 0.005 0.006 0.009 0.0070.004 0.007 53 26.9 28.0 29.6 31.5 29.6 26.8 28.5 28.2 30.5 27.6 580.005 0.011 0.018 0.008 0.008 0.009 0.014 0.010 0.007 0.010 Table 29:Provided are the values of each of the parameters (as described above)measured in Sorghum accessions (Seed ID) under low nitrogen conditions.Growth conditions are specified in the experimental procedure section.

TABLE 30 Sorghum accessions, measured parameters under 100 mM NaClgrowth conditions Corr. Seed ID ID 20 22 26 27 28 29 30 31 34 37 1 0.050.10 0.12 0.07 0.08 0.08 0.14 0.10 0.17 0.14 5 0.09 0.19 0.20 0.14 0.130.13 0.15 0.19 0.10 0.12 9 3.0 3.1 3.4 3.1 3.3 3.1 3.1 3.3 3.0 3.1 134.0 4.4 4.9 4.6 4.5 4.5 4.5 4.8 4.3 4.2 17 4.0 4.1 4.6 4.4 4.1 4.3 4.14.5 3.8 4.2 51 8.2 8.5 6.1 7.0 8.5 6.9 7.8 7.1 8.6 8.2 31 7.9 9.5 10.97.9 9.7 8.5 8.9 10.4 7.0 7.8 35 14.2 16.3 20.4 13.3 15.9 16.5 15.5 18.913.7 15.8 39 21.8 23.2 30.4 22.8 23.7 23.3 22.5 26.8 20.3 23.6 42 0.0020.003 0.004 0.002 0.002 0.003 0.004 0.003 0.005 0.004 46 0.003 0.0050.007 0.004 0.004 0.004 0.005 0.006 0.003 0.004 55 32.7 35.1 28.0 30.934.5 30.0 32.1 31.9 32.5 34.3 56 0.004 0.008 0.012 0.007 0.006 0.0070.009 0.009 0.008 0.008 Table 30: Provided are the values of each of theparameters (as described above) measured in Sorghum accessions (Seed ID)under 100 mM NaCl growth conditions. Growth conditions are specified inthe experimental procedure section.

TABLE 31 Sorghum accessions, measured parameters under cold growthconditions Corr. Seed ID ID 20 22 26 27 28 29 30 31 34 37 2 0.068 0.1080.163 0.094 0.084 0.114 0.137 0.127 0.108 0.139 6 0.078 0.154 0.1890.112 0.130 0.165 0.152 0.150 0.112 0.141 10 3.0 3.0 3.5 3.2 3.4 3.2 3.13.1 3.1 3.0 14 3.9 4.1 4.6 4.2 4.3 4.2 4.2 4.3 4.2 4.0 18 4.7 5.3 5.45.5 5.3 5.1 4.5 5.4 5.4 5.2 52 6.1 5.7 5.0 5.9 5.3 5.9 7.2 5.3 5.9 5.732 6.5 8.8 10.4 6.8 9.0 9.0 8.0 9.2 6.5 7.2 36 11.2 15.9 18.4 12.2 16.014.6 14.6 17.3 13.4 13.9 43 0.002 0.004 0.006 0.003 0.003 0.004 0.0040.004 0.003 0.005 47 0.003 0.005 0.007 0.003 0.005 0.006 0.005 0.0050.004 0.005 52 28.6 30.3 27.0 32.3 28.3 29.9 32.5 28.6 31.7 29.6 570.005 0.009 0.013 0.006 0.008 0.009 0.009 0.010 0.007 0.009 Table 31:Provided are the values of each of the parameters (as described above)measured in Sorghum accessions (Seed ID) under cold growth conditions.Growth conditions are specified in the experimental procedure section.

TABLE 32 Sorghum accessions, measured parameters under regular growthconditions Corr. Seed ID ID 20 22 26 27 28 29 30 31 34 37 4 0.05 0.130.17 0.10 0.11 0.12 0.14 0.12 0.10 0.12 8 0.10 0.24 0.31 0.16 0.19 0.190.24 0.24 0.19 0.24 12 3.0 3.1 3.8 3.2 3.2 3.2 3.1 3.4 3.0 3.0 16 4.24.5 4.8 4.6 4.5 5.0 4.6 4.9 4.5 4.6 20 5.3 5.9 6.2 5.8 5.8 5.7 5.7 6.05.6 6.1 54 5.0 5.0 4.8 5.0 4.3 4.3 5.4 4.3 5.9 5.5 21 2.0 1.9 1.9 1.61.8 1.6 1.8 2.0 1.9 2.2 22 0.9 2.2 2.8 1.7 1.8 2.0 2.3 2.0 1.1 1.9 231.7 3.9 5.1 2.6 3.2 3.1 4.0 4.0 2.0 4.0 24 2.5 6.1 8.0 4.3 4.9 5.0 6.26.0 3.1 5.9 34 7.5 9.3 12.9 8.6 8.9 8.5 10.7 10.3 7.9 8.8 38 15.0 18.222.1 17.6 18.1 18.5 22.8 22.0 20.0 21.8 41 0.16 0.19 0.16 0.17 0.17 0.170.17 0.17 0.17 0.20 45 0.002 0.005 0.006 0.004 0.004 0.005 0.005 0.0050.003 0.003 49 0.004 0.008 0.011 0.005 0.008 0.008 0.008 0.010 0.0060.007 54 26.7 29.3 29.9 29.1 25.0 24.6 30.8 25.5 32.9 33.5 59 0.0060.013 0.016 0.009 0.012 0.013 0.012 0.014 0.009 0.011 Table 32: Providedare the values of each of the parameters (as described above) measuredin Sorghum accessions (Seed ID) under regular growth conditions. Growthconditions are specified in the experimental procedure section.

TABLE 33 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under low nitrogen, normal, cold or salinitystress conditions across Sorghum accessions Gene Exp. Corr. Gene Exp.Corr. name R P set ID name R P set ID LNU433 0.76 0.0459 A 30 LNU2910.76 0.0183 I 1 LNU313 0.70 0.0340 C 53 LNU479 0.76 0.0166 I 1 LNU4800.72 0.0299 I 51 LNU401 0.74 0.0239 I 1 LNU396 0.71 0.0327 L 52 LNU3930.72 0.0271 I 1 LNU465 0.76 0.0105 J 52 LNU422 0.78 0.0123 I 1 LNU3160.75 0.0122 J 52 LNU346 0.81 0.0082 I 1 LNU432 0.75 0.0191 F 54 LNU3930.71 0.0312 L 2 LNU477 0.78 0.0141 D 54 LNU422 0.71 0.0308 L 2 LNU4320.72 0.0284 D 54 LNU481 0.79 0.0070 J 2 LNU480 0.78 0.0134 L 32 LNU2910.75 0.0197 C 3 LNU473 0.80 0.0091 L 32 LNU479 0.92 0.0004 C 3 LNU3930.75 0.0194 L 32 LNU491 0.86 0.0029 C 3 LNU422 0.83 0.0057 L 32 LNU3930.82 0.0066 C 3 LNU501 0.72 0.0289 L 32 LNU422 0.91 0.0007 C 3 LNU4790.82 0.0065 C 33 LNU346 0.81 0.0081 C 3 LNU491 0.86 0.0030 C 33 LNU4310.84 0.0050 C 3 LNU422 0.78 0.0131 C 33 LNU481 0.81 0.0265 A 3 LNU4310.86 0.0027 C 33 LNU291 0.74 0.0236 C 7 LNU439 0.74 0.0232 F 34 LNU4790.91 0.0006 C 7 LNU479 0.73 0.0252 D 34 LNU491 0.76 0.0184 C 7 LNU3950.71 0.0334 D 34 LNU393 0.74 0.0222 C 7 LNU422 0.74 0.0215 D 34 LNU4220.82 0.0068 C 7 LNU431 0.77 0.0162 D 34 LNU346 0.74 0.0219 C 7 LNU4800.79 0.0117 L 36 LNU431 0.92 0.0005 C 7 LNU387 0.74 0.0223 L 36 LNU4730.77 0.0434 A 7 LNU473 0.72 0.0303 L 36 LNU291 0.73 0.0270 L 6 LNU3930.73 0.0261 L 36 LNU480 0.71 0.0326 L 6 LNU422 0.83 0.0056 L 36 LNU3930.79 0.0110 L 6 LNU479 0.81 0.0087 C 37 LNU422 0.85 0.0034 L 6 LNU4220.71 0.0329 C 37 LNU473 0.73 0.0248 L 10 LNU431 0.80 0.0094 C 37 LNU3930.73 0.0259 L 10 LNU473 0.78 0.0378 A 37 LNU433 0.79 0.0113 L 10 LNU3970.76 0.0184 F 38 LNU291 0.76 0.0168 C 11 LNU346 0.72 0.0275 D 38 LNU4790.82 0.0073 C 11 LNU397 0.71 0.0308 I 39 LNU491 0.84 0.0050 C 11 LNU2910.75 0.0192 C 40 LNU477 0.77 0.0448 A 11 LNU479 0.83 0.0053 C 40 LNU3930.90 0.0059 A 11 LNU491 0.80 0.0094 C 40 LNU291 0.73 0.0265 F 12 LNU4220.75 0.0211 C 40 LNU291 0.83 0.0054 D 12 LNU431 0.73 0.0241 C 40 LNU3950.75 0.0210 D 12 LNU422 0.78 0.0366 A 40 LNU431 0.80 0.0089 D 12 LNU2910.73 0.0248 I 42 LNU291 0.85 0.0041 L 14 LNU479 0.78 0.0130 I 42 LNU4800.91 0.0007 L 14 LNU401 0.72 0.0298 I 42 LNU473 0.87 0.0025 L 14 LNU4220.71 0.0331 I 42 LNU393 0.92 0.0005 L 14 LNU346 0.81 0.0075 I 42 LNU4220.89 0.0012 L 14 LNU291 0.76 0.0170 L 43 LNU491 0.74 0.0150 J 14 LNU3930.78 0.0127 L 43 LNU291 0.73 0.0264 C 15 LNU422 0.78 0.0128 L 43 LNU4790.80 0.0091 C 15 LNU491 0.75 0.0124 J 43 LNU431 0.75 0.0199 C 15 LNU4810.81 0.0043 J 43 LNU422 0.89 0.0080 A 15 LNU291 0.72 0.0293 C 44 LNU4800.72 0.0293 L 18 LNU479 0.90 0.0009 C 44 LNU479 0.77 0.0143 C 19 LNU4910.84 0.0047 C 44 LNU431 0.80 0.0092 C 19 LNU393 0.83 0.0061 C 44 LNU4410.79 0.0326 A 19 LNU422 0.90 0.0009 C 44 LNU291 0.75 0.0204 C 27 LNU3460.80 0.0103 C 44 LNU479 0.82 0.0067 C 27 LNU431 0.85 0.0039 C 44 LNU4910.81 0.0088 C 27 LNU481 0.76 0.0485 A 44 LNU393 0.78 0.0129 C 27 LNU2910.79 0.0117 L 47 LNU422 0.86 0.0027 C 27 LNU480 0.71 0.0310 L 47 LNU3460.78 0.0137 C 27 LNU393 0.83 0.0057 L 47 LNU431 0.90 0.0010 C 27 LNU4220.89 0.0013 L 47 LNU387 0.90 0.0060 A 27 LNU479 0.87 0.0021 C 48 LNU4730.81 0.0275 A 27 LNU491 0.71 0.0317 C 48 LNU495 0.84 0.0181 A 50 LNU3930.72 0.0272 C 48 LNU501 0.79 0.0337 A 50 LNU422 0.79 0.0114 C 48 LNU2910.75 0.0197 C 25 LNU346 0.71 0.0314 C 48 LNU479 0.92 0.0004 C 25 LNU4310.91 0.0007 C 48 LNU491 0.86 0.0029 C 25 LNU473 0.78 0.0391 A 48 LNU3930.82 0.0066 C 25 LNU396 0.85 0.0039 L 52 LNU422 0.91 0.0007 C 25 LNU3160.87 0.0024 L 52 LNU346 0.81 0.0081 C 25 LNU396 0.79 0.0063 J 52 LNU4310.84 0.0050 C 25 LNU316 0.89 0.0006 J 52 LNU313 0.80 0.0306 A 25 LNU4770.70 0.0354 C 53 LNU481 0.84 0.0170 A 25 LNU479 0.81 0.0257 A 53 LNU3870.84 0.0167 A 25 LNU415 0.83 0.0223 A 53 LNU421 0.76 0.0465 A 25 LNU3930.86 0.0139 A 53 LNU314 0.76 0.0471 A 25 LNU324 0.78 0.0373 A 53 LNU2910.74 0.0236 C 26 LNU346 0.78 0.0401 A 53 LNU479 0.91 0.0006 C 26 LNU4730.76 0.0173 I 51 LNU491 0.76 0.0184 C 26 LNU479 0.82 0.0065 I 56 LNU3930.74 0.0222 C 26 LNU397 0.73 0.0262 I 56 LNU422 0.82 0.0068 C 26 LNU2910.80 0.0100 L 57 LNU346 0.74 0.0219 C 26 LNU393 0.83 0.0057 L 57 LNU4310.92 0.0005 C 26 LNU422 0.86 0.0027 L 57 LNU387 0.90 0.0063 A 26 LNU4910.74 0.0150 J 57 LNU473 0.85 0.0165 A 26 LNU481 0.73 0.0161 J 57 LNU4790.79 0.0106 C 28 LNU291 0.71 0.0312 C 58 LNU393 0.74 0.0224 C 28 LNU4790.90 0.0011 C 58 LNU346 0.74 0.0234 C 28 LNU491 0.77 0.0156 C 58 LNU3130.82 0.0238 A 28 LNU393 0.77 0.0148 C 58 LNU291 0.73 0.0263 C 29 LNU4220.84 0.0045 C 58 LNU479 0.75 0.0192 C 29 LNU346 0.75 0.0193 C 58 LNU2910.76 0.0170 C 30 LNU431 0.90 0.0010 C 58 LNU479 0.82 0.0068 C 30 LNU4730.76 0.0458 A 58 LNU346 0.72 0.0285 C 30 Table 33. “Corr. SetID”—correlation set ID according to the correlated parameters Tableabove. “Exp. Set” = Expression set.

Example 9 Production of Maize Transcriptom and High ThroughputCorrelation Analysis with Yield and NUE Related Parameters Using 44KMaize Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis between plantphenotype and gene expression level, the present inventors utilized amaize oligonucleotide micro-array, produced by Agilent Technologies[Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent(dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotiderepresents about 44,000 maize genes and transcripts.

Correlation of Maize Hybrids Across Ecotypes Grown Under Regular GrowthConditions

Experimental Procedures

12 Maize hybrids were grown in 3 repetitive plots, in field. Maize seedswere planted and plants were grown in the field using commercialfertilization and irrigation protocols. In order to define correlationsbetween the levels of RNA expression with NUE and yield components orvigor related parameters, the 12 different maize hybrids were analyzed.Among them, 10 hybrids encompassing the observed variance were selectedfor RNA expression analysis. The correlation between the RNA levels andthe characterized parameters was analyzed using Pearson correlation test[Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Analyzed maize tissues—All 10 selected maize hybrids were sample pereach treatment. Five types of plant tissues [flag leaf indicated inTable 34 as leaf, flower meristem, grain, Ear, and internode] growingunder Normal conditions were sampled and RNA was extracted as describedabove. Each micro-array expression information tissue type has receiveda Set ID as summarized in Table 34 below.

TABLE 34 Maize transcriptom expression sets Expression Set Set ID Maizefield/Normal/flower meristem A Maize field/Normal/Ear B Maizefield/Normal/Grain Distal C Maize field/Normal/Grain Basal D Maizefield/Normal/Internode E Maize field/Normal/Leaf F Table 34: Providedare the maize transcriptom expression sets. Leaf = the leaf below themain ear; Flower meristem = Apical meristem following male flowerinitiation; Ear = the female flower at the anthesis day. Grain Distal =maize developing grains from the cob extreme area, Grain Basal = maizedeveloping grains from the cob basal area; Internodes = internodeslocated above and below the main ear in the plant.

The following parameters were collected using digital imaging system:

Grain area (cm²)—At the end of the growing period the grains wereseparated from the ear. A sample of ˜200 grains were weight,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.

Grain Length and Grain width (cm)—At the end of the growing period thegrains were separated from the ear. A sample of −200 grains were weight,photographed and images were processed using the below described imageprocessing system. The sum of grain lengths/or width (longest axis) wasmeasured from those images and was divided by the number of grains.

Ear Area (cm²)—At the end of the growing period 5 ears were,photographed and images were processed using the below described imageprocessing system. The Ear area was measured from those images and wasdivided by the number of Ears.

Ear Length and Ear Width (cm)

At the end of the growing period 5 ears were, photographed and imageswere processed using the below described image processing system. TheEar length and width (longest axis) was measured from those images andwas divided by the number of ears.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels(3888×2592 pixels) and stored in a low compression JPEG (JointPhotographic Experts Group standard) format. Next, image processingoutput data for seed area and seed length was saved to text files andanalyzed using the JMP statistical analysis software (SAS institute).

Additional parameters were collected either by sampling 6 plants perplot or by measuring the parameter across all the plants within theplot.

Normalized Grain Weight per plant (gr.)—At the end of the experiment allears from plots within blocks A-C were collected. Six ears wereseparately threshed and grains were weighted, all additional ears werethreshed together and weighted as well. The average grain weight per earwas calculated by dividing the total grain weight by number of totalears per plot (based on plot). In case of 6 ears, the total grainsweight of 6 ears was divided by 6.

Ear FW (gr.)—At the end of the experiment (when ears were harvested)total and 6 selected ears per plots within blocks A-C were collectedseparately. The plants with (total and 6) were weighted (gr.) separatelyand the average ear per plant was calculated for total (Ear FW per plot)and for 6 (Ear FW per plant).

Plant height and Ear height—Plants were characterized for height atharvesting. In each measure, 6 plants were measured for their heightusing a measuring tape. Height was measured from ground level to top ofthe plant below the tassel. Ear height was measured from the groundlevel to the place were the main ear is located

Leaf number per plant—Plants were characterized for leaf number duringgrowing period at 5 time points. In each measure, plants were measuredfor their leaf number by counting all the leaves of 3 selected plantsper plot.

Relative Growth Rate was calculated using Formulas XI and XII (describedabove).

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed 64 days post sowing.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot. Data were taken after 46 and54 days after sowing (DPS)

Dry weight per plant—At the end of the experiment (when Inflorescencewere dry) all vegetative material from plots within blocks A-C werecollected.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours;

Harvest Index (HI) (Maize)—The harvest index was calculated usingFormula XIV.

Harvest Index=Average grain dry weight per Ear/(Average vegetative dryweight per Ear+Average Ear dry weight)  Formula XIV:

Percent Filled Ear [%]—it was calculated as the percentage of the Eararea with grains out of the total ear.

Cob diameter [cm]—The diameter of the cob without grains was measuredusing a ruler.

Kernel Row Number per Ear—The number of rows in each ear was counted.

Experimental Results

12 different maize hybrids were grown and characterized for differentparameters: The average for each of the measured parameter wascalculated using the JMP software (Tables 35-37) and a subsequentcorrelation analysis was performed (Tables 38-39). Results were thenintegrated to the database.

TABLE 35 Maize correlated parameters (vectors) Correlation setCorrelation ID SPAD 54DPS [SPAD units] 1 SPAD 46DPS [SPAD units] 2Growth Rate Leaf Num 3 Plant Height per Plot [cm] 4 Ear Height [cm] 5Leaf Number per Plant [number] 6 Ear Length [cm] 7 Percent Filled Ear[%] 8 Cob Diameter [mm] 9 Kernel Row Number per Ear [number] 10 DW perPlant [gr] 11 Ear FW per Plant [gr] 12 Normalized Grain Weight per plant[gr] 13 Ears FW per plot [gr] 14 Normalized Grain Weight per plot [gr]15 Ear Area [cm2] 16 Ear Width [cm] 17 Grain Area [cm2] 18 Grain Length[cm] 19 Grain Width [cm] 20 Table 35. SPAD 46DPS and SPAD 54DPS:Chlorophyl level after 46 and 54 days after sowing (DPS).

TABLE 36 Measured parameters in Maize accessions under normal conditionsSeed ID 1 2 3 4 5 6 7 8 9 10 11 Line 1 54.8 55.3 0.306 287 135 11.9 20.980.4 28.7 16.2 656 Line 2 54.3 51.7 0.283 278 135 12 19.7 80.6 29 16.2658 Line 3 57.2 56.4 0.221 270 116 8.4 19.1 94.3 23.8 15 472 Line 4 5653.5 0.281 275 132 11.7 20.5 82.1 28.1 16.2 641 Line 5 59.7 55.2 0.269238 114 11.8 21.3 92.7 25.7 15.9 581 Line 6 59.1 59.4 0.244 225 94.312.3 18.2 82.8 25.8 15.2 569 Line 7 58 58.5 0.244 264 121 12.4 19 73.226.4 16 511 Line 8 60.4 55.9 0.266 252 108 12.2 18.6 81.1 25.2 14.8 544Line 9 54.8 53 Line 10 53.3 50 Line 11 61.1 59.7 0.301 278 112 12.6 21.791.6 26.7 15.4 522 Line 12 51.4 53.9 0.194 164 60.4 9.28 16.7 81.1 14.3574 141 Table 36. Provided are the values of each of the parameters (asdescribed above) measured in maize accessions (Seed ID) under regulargrowth conditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 37 Additional measured parameters in Maize accessions underregular growth conditions Seed ID 12 13 14 15 16 17 18 19 20 Line 1 272157 280 140 91.6 5.73 0.806 1.23 0.824 Line 2 246 141 278 154 85.1 5.580.753 1.17 0.81 Line 3 190 129 190 121 77.9 5.1 0.674 1.07 0.794 Line 4262 154 288 152 90.5 5.67 0.755 1.18 0.803 Line 5 264 177 248 159 965.53 0.766 1.2 0.803 Line 6 178 120 176 117 72.4 5.23 0.713 1.12 0.803Line 7 189 120 192 123 74 5.22 0.714 1.14 0.791 Line 8 197 134 205 13176.5 5.33 0.753 1.13 0.837 Line 9 Line 10 Line 11 261 173 264 171 95.45.58 0.762 1.18 0.812 Line 12 54.3 143 40.8 55.2 4.12 0.796 0.921 0.675Table 37. Provided are the values of each of the parameters (asdescribed above) measured in maize accessions (Seed ID) under regulargrowth conditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 38 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under normal across maize accessions Gene Exp.Corr. Gene Exp. Corr. Name R P set ID Name R P set ID LNU348 0.79000.0196 C 3 LNU394 0.8881 0.0076 B 14 LNU348 0.7286 0.0404 C 18 LNU3940.8830 0.0084 B 19 LNU348 0.7222 0.0430 C 17 LNU394 0.8753 0.0099 B 13LNU394 0.8524 0.0035 E 8 LNU394 0.8628 0.0124 B 16 LNU394 0.7673 0.0158E 6 LNU394 0.8616 0.0127 B 17 LNU394 0.7621 0.0170 E 15 LNU394 0.85680.0138 B 15 LNU299 0.8148 0.0075 E 6 LNU394 0.7824 0.0376 B 18 LNU3000.7810 0.0130 E 6 LNU361 0.8667 0.0116 B 10 LNU307 0.9065 0.0008 E 8LNU361 0.8514 0.0151 B 19 LNU307 0.8849 0.0015 E 15 LNU361 0.8239 0.0227B 17 LNU307 0.8768 0.0019 E 13 LNU361 0.7833 0.0372 B 8 LNU307 0.87450.0020 E 20 LNU361 0.7763 0.0401 B 18 LNU307 0.8402 0.0046 E 16 LNU3610.7697 0.0430 B 6 LNU307 0.8205 0.0067 E 18 LNU299 0.8970 0.0062 B 3LNU307 0.8051 0.0088 E 17 LNU299 0.8346 0.0195 B 6 LNU307 0.7843 0.0123E 4 LNU299 0.8064 0.0285 B 17 LNU307 0.7528 0.0192 E 19 LNU299 0.77730.0397 B 19 LNU307 0.7262 0.0267 E 5 LNU299 0.7740 0.0411 B 8 LNU3010.7753 0.0141 E 6 LNU299 0.7657 0.0448 B 20 LNU317 0.8504 0.0037 E 19LNU360 0.8629 0.0124 B 20 LNU317 0.8352 0.0051 E 3 LNU300 0.9181 0.0035B 15 LNU317 0.8118 0.0079 E 7 LNU300 0.8850 0.0081 B 13 LNU317 0.80060.0095 E 6 LNU300 0.8730 0.0103 B 8 LNU317 0.7927 0.0108 E 18 LNU3000.8529 0.0147 B 4 LNU317 0.7747 0.0142 E 17 LNU300 0.8041 0.0293 B 5LNU317 0.7506 0.0198 E 13 LNU300 0.8018 0.0301 B 16 LNU317 0.7433 0.0217E 4 LNU300 0.8007 0.0305 B 3 LNU317 0.7043 0.0342 E 12 LNU300 0.79520.0325 B 6 LNU394 0.8756 0.0098 E 10 LNU300 0.7952 0.0325 B 20 LNU3940.8714 0.0106 E 19 LNU359 0.9025 0.0054 B 19 LNU394 0.8557 0.0140 E 3LNU359 0.9020 0.0055 B 17 LNU394 0.8451 0.0167 E 7 LNU359 0.9006 0.0057B 20 LNU394 0.8200 0.0239 E 17 LNU359 0.8219 0.0233 B 15 LNU394 0.80990.0273 E 13 LNU359 0.7847 0.0367 B 13 LNU394 0.8079 0.0279 E 12 LNU3070.8759 0.0097 B 5 LNU394 0.7847 0.0366 E 15 LNU307 0.8742 0.0101 B 17LNU394 0.7610 0.0469 E 16 LNU307 0.8602 0.0130 B 15 LNU394 0.7560 0.0493E 18 LNU307 0.8592 0.0132 B 4 LNU394 0.7545 0.0500 E 6 LNU307 0.84600.0164 B 19 LNU361 0.9074 0.0048 E 3 LNU307 0.8299 0.0209 B 6 LNU3600.8408 0.0178 E 4 LNU307 0.8151 0.0255 B 13 LNU360 0.8050 0.0289 E 5LNU460 0.9092 0.0045 B 6 LNU300 0.7717 0.0421 E 8 LNU460 0.9050 0.0051 B20 LNU300 0.7696 0.0430 E 6 LNU460 0.8531 0.0147 B 8 LNU300 0.76750.0440 E 18 LNU460 0.8499 0.0154 B 18 LNU300 0.7663 0.0445 E 19 LNU4600.7765 0.0401 B 19 LNU476 0.8604 0.0130 E 10 LNU460 0.7754 0.0405 B 17LNU307 0.8902 0.0072 E 18 LNU460 0.7733 0.0414 B 15 LNU307 0.8670 0.0115E 8 LNU418 0.8317 0.0203 B 10 LNU307 0.8499 0.0154 E 5 LNU469 0.87250.0104 B 14 LNU307 0.8324 0.0202 E 4 LNU469 0.8678 0.0113 B 12 LNU3070.8128 0.0262 E 17 LNU469 0.8634 0.0123 B 16 LNU307 0.8021 0.0300 E 15LNU469 0.8140 0.0259 B 7 LNU307 0.7789 0.0390 E 19 LNU469 0.7969 0.0319B 5 LNU307 0.7744 0.0409 E 6 LNU301 0.8989 0.0059 B 8 LNU332 0.91090.0043 E 4 LNU471 0.8820 0.0086 B 20 LNU332 0.8808 0.0088 E 5 LNU4710.8585 0.0134 B 8 LNU332 0.8349 0.0194 E 3 LNU471 0.8071 0.0282 B 18LNU332 0.8297 0.0209 E 14 LNU471 0.8037 0.0294 B 6 LNU332 0.8057 0.0287E 17 LNU317 0.8799 0.0090 B 14 LNU332 0.7983 0.0314 E 15 LNU317 0.79480.0327 B 12 LNU332 0.7822 0.0377 E 18 LNU317 0.7734 0.0414 B 7 LNU4590.7966 0.0320 E 20 LNU371 0.8211 0.0235 B 6 LNU519 0.8564 0.0139 E 8LNU371 0.8036 0.0295 B 3 LNU519 0.7634 0.0458 E 20 LNU371 0.7612 0.0468B 19 LNU519 0.7596 0.0476 E 6 LNU311 0.8585 0.0134 B 10 LNU317 0.84100.0177 E 3 LNU311 0.8191 0.0242 B 17 LNU371 0.7717 0.0421 E 6 LNU3110.7814 0.0380 B 19 LNU394 0.7595 0.0288 E 7 LNU361 0.8460 0.0081 C 9LNU299 0.7110 0.0480 E 8 LNU361 0.8178 0.0131 C 11 LNU476 0.7456 0.0337E 6 LNU361 0.8114 0.0145 C 3 LNU317 0.7611 0.0283 E 10 LNU361 0.74510.0339 C 17 LNU317 0.7294 0.0400 E 19 LNU361 0.7393 0.0361 C 18 LNU3710.8610 0.0060 E 6 LNU299 0.8498 0.0076 C 9 LNU394 0.7058 0.0226 F 7LNU299 0.8299 0.0108 C 4 LNU299 0.7097 0.0215 F 4 LNU299 0.7977 0.0177 C5 LNU300 0.7357 0.0153 F 6 LNU299 0.7974 0.0178 C 3 LNU476 0.7907 0.0065F 13 LNU299 0.7645 0.0271 C 11 LNU476 0.7657 0.0098 F 15 LNU299 0.72390.0423 C 14 LNU476 0.7627 0.0103 F 16 LNU299 0.7196 0.0442 C 17 LNU3070.9158 0.0002 F 20 LNU360 0.8298 0.0108 C 9 LNU307 0.8603 0.0014 F 18LNU360 0.7486 0.0326 C 11 LNU307 0.8063 0.0048 F 5 LNU360 0.7461 0.0335C 3 LNU307 0.7910 0.0064 F 19 LNU359 0.8130 0.0141 C 9 LNU307 0.78650.0070 F 4 LNU359 0.7391 0.0362 C 3 LNU307 0.7827 0.0074 F 17 LNU3590.7328 0.0387 C 11 LNU307 0.7600 0.0107 F 15 LNU476 0.8702 0.0049 C 18LNU307 0.7562 0.0114 F 6 LNU476 0.8498 0.0075 C 3 LNU307 0.7331 0.0159 F13 LNU476 0.7860 0.0207 C 19 LNU307 0.7158 0.0199 F 8 LNU476 0.71850.0447 C 17 LNU459 0.7659 0.0098 F 6 LNU332 0.8630 0.0058 C 9 LNU4590.7382 0.0148 F 20 LNU332 0.8522 0.0072 C 11 LNU459 0.7201 0.0188 F 5LNU332 0.8394 0.0092 C 3 LNU317 0.7722 0.0089 F 13 LNU332 0.7634 0.0275C 17 LNU317 0.7293 0.0167 F 7 LNU332 0.7277 0.0407 C 18 LNU317 0.71090.0212 F 16 LNU332 0.7093 0.0488 C 14 LNU394 0.8690 0.0111 F 3 LNU4600.7756 0.0237 C 9 LNU394 0.8665 0.0116 F 7 LNU460 0.7209 0.0436 C 3LNU394 0.8008 0.0305 F 12 LNU418 0.8127 0.0142 C 5 LNU394 0.7609 0.0470F 14 LNU418 0.7625 0.0278 C 4 LNU360 0.7551 0.0497 F 16 LNU301 0.81530.0137 C 9 LNU300 0.8481 0.0159 F 19 LNU301 0.7645 0.0272 C 4 LNU3000.8044 0.0291 F 18 LNU301 0.7126 0.0473 C 5 LNU300 0.8044 0.0292 F 17LNU471 0.9147 0.0015 C 17 LNU300 0.7981 0.0314 F 13 LNU471 0.8947 0.0027C 11 LNU300 0.7790 0.0390 F 15 LNU471 0.8818 0.0038 C 18 LNU300 0.75460.0499 F 8 LNU471 0.8772 0.0042 C 19 LNU307 0.9128 0.0041 F 8 LNU4710.8494 0.0076 C 9 LNU307 0.8940 0.0066 F 18 LNU471 0.8027 0.0165 C 12LNU307 0.8476 0.0160 F 17 LNU471 0.7913 0.0193 C 14 LNU307 0.8476 0.0160F 6 LNU471 0.7083 0.0493 C 10 LNU307 0.8300 0.0208 F 19 LNU339 0.81430.0139 C 9 LNU307 0.7857 0.0362 F 5 LNU339 0.7695 0.0256 C 11 LNU3070.7766 0.0400 F 15 LNU339 0.7123 0.0474 C 3 LNU307 0.7590 0.0479 F 1LNU519 0.7864 0.0206 C 11 LNU469 0.8415 0.0176 F 16 LNU519 0.7631 0.0276C 9 LNU469 0.8375 0.0187 F 12 LNU519 0.7345 0.0380 C 3 LNU469 0.80170.0301 F 10 LNU371 0.8650 0.0055 C 4 LNU469 0.7995 0.0309 F 7 LNU3710.8176 0.0132 C 5 LNU469 0.7727 0.0417 F 13 LNU311 0.8403 0.0090 C 9LNU469 0.7727 0.0417 F 19 LNU311 0.7667 0.0264 C 11 LNU469 0.7557 0.0494F 14 LNU348 0.8430 0.0086 C 9 LNU371 0.7611 0.0469 F 6 LNU348 0.80360.0163 C 11 LNU394 0.8917 0.0070 B 10 Table 38. “Corr. SetID”—correlation set ID according to the correlated parameters Tableabove.

TABLE 39 Correlation between the expression level of selected LNUhomologous genes of some embodiments of the invention in various tissuesand the phenotypic performance under normal across maize accessions GeneExp. Corr. Gene Exp. Corr. Name R P set ID Name R P set ID LNU309_H30.84 0.0050 E 6 LNU431_H1 0.89 0.0078 B 20 LNU309_H3 0.76 0.0165 E 19LNU431_H1 0.78 0.0371 B 18 LNU309_H3 0.76 0.0182 E 3 LNU431_H1 0.760.0464 B 8 LNU309_H3 0.74 0.0221 E 18 LNU417_H4 0.81 0.0159 C 9LNU494_H2 0.76 0.0459 E 19 LNU417_H4 0.74 0.0365 C 11 LNU309_H3 0.740.0138 F 5 LNU417_H4 0.73 0.0391 C 3 LNU309_H3 0.73 0.0173 F 14LNU431_H1 0.71 0.0486 C 3 Table 39. “Corr. Set ID”—correlation set IDaccording to the correlated parameters Table above. “Exp. set” =Expression set.

Example 10 Production of Tomato Transcriptom and High ThroughputCorrelation Analysis Using 44K Tomato Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis between NUErelated phenotypes and gene expression, the present inventors utilized aTomato oligonucleotide micro-array, produced by Agilent Technologies[Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent(dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotiderepresents about 44,000 Tomato genes and transcripts. In order to definecorrelations between the levels of RNA expression with NUE, ABST, yieldcomponents or vigor related parameters various plant characteristics of18 different Tomato varieties were analyzed. Among them, 10 varietiesencompassing the observed variance were selected for RNA expressionanalysis. The correlation between the RNA levels and the characterizedparameters was analyzed using Pearson correlation test [HypertextTransfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Correlation of Tomato Varieties Across Ecotypes Grown Under LowNitrogen, Drought and Regular Growth Conditions

Experimental Procedures:

10 Tomato varieties were grown in 3 repetitive blocks, each containing 6plants per plot were grown at net house. Briefly, the growing protocolwas as follows:

1. Regular growth conditions: Tomato varieties were grown under normalconditions (4-6 Liters/m² of water per day and fertilized with NPK asrecommended in protocols for commercial tomato production).

2. Low Nitrogen fertilization conditions: Tomato varieties were grownunder normal conditions (4-6 Liters/m² per day and fertilized with NPKas recommended in protocols for commercial tomato production) untilflowering. At this time, Nitrogen fertilization was stopped.

3. Drought stress: Tomato variety was grown under normal conditions (4-6Liters/m² per day) until flowering. At this time, irrigation was reducedto 50% compared to normal conditions. Plants were phenotyped on a dailybasis following the standard descriptor of tomato (Table 40). Harvestwas conducted while 50% of the fruits were red (mature). Plants wereseparated to the vegetative part and fruits, of them, 2 nodes wereanalyzed for additional inflorescent parameters such as size, number offlowers, and inflorescent weight. Fresh weight of all vegetativematerial was measured. Fruits were separated to colors (red vs. green)and in accordance with the fruit size (small, medium and large). Next,analyzed data was saved to text files and processed using the JMPstatistical analysis software (SAS institute). Data parameters collectedare summarized in Table 41, herein below.

Analyzed tomato tissues—Two tissues at different developmental stages[flower and leaf], representing different plant characteristics, weresampled and RNA was extracted as described above. For convenience, eachmicro-array expression information tissue type has received a Set ID assummarized in Table 40 below.

TABLE 40 Tomato transcriptom expression sets Expression Set Set ID Leafgrown under Normal Conditions A Leaf grown under 50% Irrigation B Flowergrown under Normal Conditions C Flower grown under 50% Irrigation D Leafgrown under Low Nitrogen E Flower grown under Low Nitrogen F Table 40:Provided are the identification (ID) letters of each of the tomatoexpression sets.

The average for each of the measured parameter was calculated using theJMP software and values are summarized in Tables 42-47 below. Subsequentcorrelation analysis was conducted (Table 48) with the correlationcoefficient (R) and the p-values. Results were integrated to thedatabase.

TABLE 41 Tomato correlated parameters (vectors) Correlation setCorrelation ID average red fruit weight (Normal) [gr.] 1 average redfruit weight (NUE) [gr.] 2 average red fruit weight Drought [gr.] 3flower cluster weight Drought/NUE 4 Fruit yield/Plant (Normal) [gr.] 5Fruit Yield/Plant (Drought) [gr.] 6 Fruit Yield/Plant (NUE) [gr.] 7 FWratio (Drought/Normal) 8 FW ratio (NUE/Normal) 9 FW/Plant (Normal) [gr.]10 FW/Plant (NUE) [gr.] 11 FW/Plant Drought [gr.] 12 HI (Low N) 13 HI(Normal) 14 Leaflet Length [cm] (Low N) 15 Leaflet Length [cm] (Normal)16 Leaflet Width (Low N) 17 Leaflet Width (Normal) 18 No flowers(Normal) [number] 19 No flowers (NUE) [number] 20 NUE [yield/SPAD] (LowN) 21 NUE [yield/SPAD] (Normal) 22 NUE2 [total biomass/SPAD] (Low N) 23NUE2 [total biomass/SPAD] (Normal) 24 Num of flowers (Drought) [number]25 Num. Flowers NUE/Normal 26 NUpE [biomass/SPAD] (Low N) 27 NUpE[biomass/SPAD] (Normal) 28 Ratio of Cluster Weight (NUE/Normal) 29 Ratioof Flower Cluster Weight (Drought/Normal) 30 Ratio of Fruit Yield(Drought/Normal) 31 Ratio of Fruits (Drought/NUE) 32 Ratio of Fruits(NUE/Normal) 33 Ratio of Number of Flowers (Drought/Normal) 34 Ratio ofNumber of Flowers (Drought/NUE) 35 Ratio of RWC (NUE/Normal) 36 Ratio ofSPAD (NUE/Normal) 37 Ratio of SPAD 100% RWC (NUE/Normal) 38 red fruitweight Drought/Normal 39 RWC (Normal) [%] 40 RWC Drought [%] 41 RWCDrought/Normal 42 RWC NUE [%] 43 SLA [leaf area/plant biomass] (Low N)44 SLA [leaf area/plant biomass] (Normal) 45 SPAD (Normal) [SPAD unit]46 SPAD 100% RWC (Normal) [SPAD unit] 47 SPAD 100% RWC (NUE) [SPAD unit]48 SPAD NUE [SPAD unit] 49 Total Leaf Area [cm{circumflex over ( )}2](Low N) 50 Total Leaf Area [cm2{circumflex over ( )}] (Normal) 51 Weightclusters (flowers) (NUE) [gr.] 52 Weight flower clusters (Drought) [gr.]53 Weight Flower clusters (Normal) [gr.] 54 Weight of 100 green fruits(Normal) 55 Weight of 100 green fruits (NUE) 56 Weight of 100 red fruits(Normal) 57 Weight of 100 red fruits (NUE) 58 Yield/SLA (Low N) 59Yield/SLA (Normal) 60 Yield/total leaf area (Low N) 61 Yield/total leafarea (Normal) 62 Table 41. Provided are the tomato correlatedparameters, RWC means relative water content, NUpE-nitrogen uptakeefficiency, HI-harvest index (vegetative weight divided on yield),SLA-specific leaf area (leaf area divided on leaf dry weight).

Fruit Yield (grams)—At the end of the experiment [when 50% of the fruitwere ripe (red)] all fruits from plots within blocks A-C were collected.The total fruits were counted and weighted. The average fruits weightwas calculated by dividing the total fruit weight by the number offruits.

Plant Fresh Weight (grams)—At the end of the experiment [when 50% of thefruit were ripe (red)] all plants from plots within blocks A-C werecollected. Fresh weight was measured (grams).

Inflorescence Weight (grams)—At the end of the experiment [when 50% ofthe fruits were ripe (red)] two Inflorescence from plots within blocksA-C were collected. The Inflorescence weight (gr.) and number of flowersper inflorescence were counted.

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed at time of flowering.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot.

Water use efficiency (WUE)—can be determined as the biomass produced perunit transpiration. To analyze WUE, leaf relative water content wasmeasured in control and transgenic plants. Fresh weight (FW) wasimmediately recorded; then leaves were soaked for 8 hours in distilledwater at room temperature in the dark, and the turgid weight (TW) wasrecorded. Total dry weight (DW) was recorded after drying the leaves at60° C. to a constant weight. Relative water content (RWC) was calculatedaccording to the following Formula I [(FW−DW/TW−DW)×100] as describedabove.

Plants that maintain high relative water content (RWC) compared tocontrol lines were considered more tolerant to drought than thoseexhibiting reduced relative water content

Experimental Results

TABLE 42 Measured parameters in Tomato accessions under droughtconditions Seed Corr. ID ID 41 42 25 53 34 35 30 4 612 72.1 0.99 16.70.37 2.94 0.88 0.32 0.69 613 74.5 0.97 6.5 0.41 0.34 1.22 1.19 1.11 61465.3 1.02 15.7 0.33 2.47 1.74 0.47 1.06 616 72.2 1.08 20.3 0.29 2.651.56 0.01 0.82 617 66.1 1.21 11.7 0.55 1.21 1.09 1.25 1.16 618 68.3 0.8825.3 0.31 3.04 1.52 0.03 1.25 620 78.1 1.34 29.7 0.45 5.95 4.96 0.561.52 621 18.5 0.28 17.3 0.56 2.08 1.08 0.96 1.19 622 73.2 1.13 14.7 0.301.47 0.98 0.42 0.76 623 62.5 0.83 29.7 0.32 4.24 4.94 0.38 1.04 624 67.21.01 15.0 0.31 1.67 0.88 0.36 0.38 625 75.8 1.20 10.3 0.31 1.29 0.800.62 0.78 626 62.8 1.11 18.3 8.36 3.44 2.12 8.20 24.10 627 70.7 1.9712.0 0.29 1.50 1.29 0.41 0.67 628 55.8 0.72 20.3 0.34 2.65 1.61 0.910.97 629 75.2 0.75 12.7 0.44 1.41 1.90 0.67 0.99 630 63.7 1.01 12.7 0.271.19 1.36 0.38 0.95 631 62.3 0.83 11.3 0.43 1.26 1.42 1.31 0.91 Table42: Provided are the values of each of the parameters (as describedabove) measured in Sorghum accessions (Seed ID) under droughtconditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 43 Additional Measured parameters in Tomato accessions underdrought conditions Seed Corr. ID ID 6 12 3 31 32 8 39 612 0.47 2.620.009 0.57 1.15 1.72 0.19 613 0.48 1.09 0.195 1.41 0.73 0.34 24.40 6140.63 1.85 0.209 1.27 1.32 0.61 25.40 616 0.35 2.22 0.005 2.88 0.76 2.630.02 617 2.04 2.63 0.102 4.20 1.51 1.18 20.30 618 0.25 2.71 0.002 0.550.71 1.36 0.04 620 0.05 3.41 0.035 0.09 5.06 4.02 0.15 621 0.45 2.110.006 1.03 0.89 1.01 0.02 622 0.29 1.95 0.005 1.39 0.67 0.61 0.86 6231.02 1.76 0.005 3.28 2.17 0.64 0.74 624 0.60 1.72 0.005 0.91 0.38 0.950.09 625 0.49 1.92 0.012 2.62 1.27 0.51 1.72 626 0.27 2.21 0.005 0.320.84 1.17 0.17 627 0.68 3.73 0.006 2.48 1.51 1.94 0.02 628 0.14 0.750.303 0.41 0.98 0.35 10.50 629 0.53 1.76 0.138 1.62 1.34 1.06 27.90 6300.55 0.63 0.041 1.76 0.38 0.21 11.80 631 0.41 1.11 0.089 1.42 0.84 0.489.98 Table 43.

TABLE 44 Measured parameters in Tomato accessions under normalconditions Seed Corr. ID ID 5 10 1 46 40 47 19 54 22 28 612 0.83 1.530.05 49.7 72.8 36.2 5.7 1.2 0.017 0.031 613 0.34 3.17 0.01 37.2 76.528.4 19.3 0.3 0.009 0.085 614 0.49 3.02 0.01 55.8 64.3 35.9 6.3 0.70.009 0.054 616 0.12 0.84 0.29 46.4 67.1 31.1 7.7 0.003 0.018 617 0.492.24 0.01 48.2 54.8 26.4 9.7 0.4 0.010 0.046 618 0.45 1.98 0.05 43.477.6 33.7 8.3 0.011 0.046 620 0.53 0.85 0.23 42.9 58.2 25.0 5.0 0.80.012 0.020 621 0.44 2.09 0.29 53.3 66.5 35.5 8.3 0.6 0.008 0.039 6220.21 3.21 0.01 58.5 64.7 37.9 10.0 0.7 0.004 0.055 623 0.31 2.75 0.0151.1 75.2 38.4 7.0 0.8 0.006 0.054 624 0.66 1.81 0.06 40.0 66.2 26.5 9.00.9 0.017 0.045 625 0.19 3.77 0.01 47.6 63.2 30.1 8.0 0.5 0.004 0.079626 0.85 1.89 0.03 57.9 56.8 32.9 5.3 1.0 0.015 0.033 627 0.27 1.93 0.2648.3 36.0 17.4 8.0 0.7 0.006 0.040 628 0.35 2.14 0.03 43.6 77.6 33.8 7.70.4 0.008 0.049 629 0.33 1.65 0.00 54.5 100.0 54.5 9.0 0.7 0.006 0.030630 0.31 3.01 0.00 41.6 63.2 26.3 10.7 0.7 0.008 0.072 631 0.29 2.290.01 59.1 75.1 44.4 9.0 0.3 0.005 0.039 Table 44: Provided are thevalues of each of the parameters (as described above) measured inSorghum accessions (Seed ID) under normal growth conditions. Growthconditions are specified in the experimental procedure section.

TABLE 45 Additional measured parameters in Tomato accessions undernormal conditions Corr. ID Seed ID 14 24 51 16 18 55 57 45 62 60 6120.35 0.05 613 0.10 0.09 614 0.14 0.06 426 6.3 3.7 0.6 0.82 141 0.00120.0035 616 0.13 0.02 582 8.0 4.8 3.1 2.46 690 0.0002 0.0002 617 0.180.06 291 5.6 3.4 0.2 0.50 130 0.0017 0.0037 618 0.19 0.06 594 7.7 4.62.6 2.76 299 0.0008 0.0015 620 0.38 0.03 948 7.9 4.4 6.3 5.32 11200.0006 0.0005 621 0.17 0.05 233 6.2 3.2 5.8 5.24 112 0.0019 0.0039 6220.06 0.06 341 6.2 3.4 0.4 0.61 106 0.0006 0.0020 623 0.10 0.06 339 5.73.1 0.3 0.66 123 0.0009 0.0025 624 0.27 0.06 190 4.4 2.4 2.0 2.70 1050.0035 0.0063 625 0.05 0.08 422 4.4 2.0 2.5 0.70 112 0.0004 0.0017 6260.31 0.05 581 6.8 3.8 1.4 2.64 308 0.0015 0.0028 627 0.12 0.05 808 7.43.7 2.0 4.67 419 0.0003 0.0007 628 0.14 0.06 784 6.7 3.0 1.4 2.17 3660.0004 0.0009 629 0.17 0.04 352 5.9 3.2 2.3 0.49 213 0.0009 0.0015 6300.09 0.08 256 4.2 2.1 0.5 0.34 85 0.0012 0.0037 631 0.11 0.04 1080 10.35.9 0.4 0.75 470 0.0003 0.0006 Table 45: Provided are the values of eachof the parameters (as described above) measured in Sorghum accessions(Seed ID) under normal growth conditions. Growth conditions arespecified in the experimental procedure section.

TABLE 46 Measured parameters in Tomato accessions under low nitrogenconditions Seed Corr. ID ID 7 11 2 33 9 49 43 48 37 38 36 20 52 26 6120.41 4.04 0.024 0.49 2.65 38.4 74.1 28.5 0.77 0.79 1.0 19.0 0.53 3.35613 0.66 1.21 0.191 1.93 0.38 39.4 99.1 39.0 1.06 1.37 1.3 5.3 0.37 0.28614 0.48 2.25 0.006 0.97 0.74 47.5 69.5 33.0 0.85 0.92 1.1 9.0 0.31 1.42616 0.46 2.54 0.005 3.80 3.01 37.0 63.2 23.4 0.80 0.75 0.9 13.0 0.351.70 617 1.35 1.85 0.096 2.78 0.83 44.6 77.4 34.5 0.93 1.31 1.4 10.70.47 1.10 618 0.35 3.06 0.004 0.78 1.54 41.7 77.9 32.5 0.96 0.97 1.016.7 0.25 2.00 620 0.01 3.13 0.006 0.02 3.70 34.4 80.5 27.7 0.80 1.111.4 6.0 0.29 1.20 621 0.51 2.54 0.007 1.16 1.22 50.0 67.4 33.7 0.94 0.951.0 16.0 0.47 1.92 622 0.44 1.84 0.006 2.07 0.58 44.7 67.2 30.0 0.760.79 1.0 15.0 0.40 1.50 623 0.47 1.52 0.013 1.51 0.55 53.7 66.1 35.51.05 0.92 0.9 6.0 0.30 0.86 624 1.59 1.91 0.021 2.41 1.06 35.7 69.6 24.80.89 0.94 1.1 17.0 0.82 1.89 625 0.39 1.86 0.005 2.06 0.49 58.8 69.340.8 1.24 1.36 1.1 13.0 0.40 1.62 626 0.32 2.47 0.006 0.38 1.31 47.5100.0 47.5 0.82 1.44 1.8 8.7 0.35 1.62 627 0.45 2.62 0.048 1.64 1.3645.2 57.7 26.1 0.94 1.50 1.6 9.3 0.43 1.17 628 0.14 1.08 0.357 0.41 0.5139.0 90.8 35.4 0.89 1.05 1.2 12.7 0.35 1.65 629 0.40 1.17 0.037 1.210.71 45.0 68.0 30.6 0.83 0.56 0.7 6.7 0.45 0.74 630 1.44 0.92 0.626 4.590.31 65.3 59.6 39.0 1.57 1.48 0.9 9.3 0.28 0.88 631 0.50 1.09 1.70 0.4751.9 72.2 37.5 0.88 0.84 1.0 8.0 0.47 0.89 Table 46: Provided are thevalues of each of the parameters (as described above) measured inSorghum accessions (Seed ID) under low nitrogen growth conditions.Growth conditions are specified in the experimental procedure section.

TABLE 47 Additional measured parameters in Tomato accessions under lownitrogen conditions Seed Corr. ID ID 29 21 27 13 23 50 15 17 56 44 61 5958 612 0.46 0.014 0.14 0.09 0.16 566 6.4 3.5 0.87 140 0.0007 0.003 1.1613 1.07 0.017 0.03 0.35 0.05 385 5.9 2.0 3.66 317 0.0017 0.002 6.9 6140.44 0.014 0.07 0.18 0.08 295 3.7 1.8 0.57 131 0.0016 0.004 0.6 616 0.010.020 0.11 0.15 0.13 378 5.4 2.6 0.37 149 0.0012 0.003 0.5 617 1.080.039 0.05 0.42 0.09 476 7.0 3.5 3.40 258 0.0028 0.005 7.2 618 0.020.011 0.09 0.10 0.11 197 3.7 1.7 0.68 64 0.0018 0.006 0.4 620 0.37 0.0000.11 0.00 0.11 453 4.4 1.9 0.45 145 0.0000 0.000 621 0.81 0.015 0.080.17 0.09 626 6.7 3.5 0.47 246 0.0008 0.002 0.6 622 0.55 0.015 0.06 0.190.08 748 6.7 3.3 0.54 406 0.0006 0.001 0.7 623 0.36 0.013 0.04 0.24 0.06454 4.4 2.5 0.39 299 0.0010 0.002 0.6 624 0.95 0.064 0.08 0.45 0.14 1653.9 2.6 0.97 86 0.0097 0.019 1.3 625 0.80 0.010 0.05 0.17 0.06 338 5.32.6 0.91 182 0.0012 0.002 1.3 626 0.34 0.007 0.05 0.12 0.06 396 6.3 3.60.36 160 0.0008 0.002 0.5 627 0.61 0.017 0.10 0.15 0.12 236 5.1 2.6 0.3590 0.0019 0.005 0.6 628 0.94 0.004 0.03 0.12 0.03 175 4.7 2.5 0.57 1610.0008 0.001 0.9 629 0.68 0.013 0.04 0.25 0.05 442 6.8 3.4 4.38 3790.0009 0.001 6.2 630 0.40 0.037 0.02 0.61 0.06 489 7.1 3.3 2.02 5310.0030 0.003 3.7 631 1.44 0.013 0.03 0.31 0.04 708 8.2 3.7 8.13 6510.0007 0.001 11.3 Table 47: Provided are the values of each of theparameters (as described above) measured in Sorghum accessions (Seed ID)under low nitrogen growth conditions. Growth conditions are specified inthe experimental procedure section.

TABLE 48 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under low nitrogen, normal or drought stressconditions across Tomato accessions Gene Exp. set Exp. set Gene Exp. setName R P ID Corr. ID ID Name R P ID LNU323 0.75 0.0116 F 21 F LNU3300.75 0.0127 F LNU429 0.73 0.0156 F 21 F LNU390 0.90 0.0003 F LNU310 0.800.0051 F 21 F LNU405 0.91 0.0003 F LNU461 0.71 0.0313 C 22 C LNU411 0.920.0002 F LNU328 0.84 0.0046 A 22 A LNU356 0.75 0.0134 E LNU405 0.700.0235 F 23 F LNU390 0.72 0.0178 E LNU357 0.74 0.0238 C 24 C LNU405 0.770.0094 E LNU331 0.82 0.0073 C 24 C LNU413 0.73 0.0156 F LNU383 0.780.0131 C 24 C LNU356 0.83 0.0032 E LNU342 0.70 0.0339 A 24 A LNU405 0.840.0026 E LNU430 0.71 0.0215 B 34 B LNU413 0.83 0.0112 C LNU455 0.860.0013 D 35 D LNU500 0.72 0.0187 E LNU506 0.75 0.0129 B 35 B LNU329 0.750.0128 E LNU468 0.75 0.0134 B 35 B LNU295 0.81 0.0157 C LNU430 0.790.0066 B 35 B LNU413 0.90 0.0020 C LNU489 0.71 0.0204 B 35 B LNU413 0.800.0058 F LNU455 0.84 0.0026 B 35 B LNU411 0.85 0.0017 F LNU455 0.760.0112 D 25 D LNU384 0.78 0.0072 E LNU430 0.72 0.0189 B 25 B LNU302 0.770.0095 A LNU357 0.76 0.0181 C 28 C LNU468 0.72 0.0294 F LNU331 0.780.0141 C 28 C LNU370 0.77 0.0250 C LNU383 0.74 0.0231 C 28 C LNU468 0.820.0038 C LNU375 0.72 0.0297 C 28 C LNU375 0.82 0.0071 F LNU430 0.730.0252 C 28 C LNU430 0.72 0.0294 F LNU342 0.75 0.0209 A 28 A LNU390 0.810.0043 E LNU461 0.76 0.0106 D 41 D LNU411 0.72 0.0183 E LNU384 0.790.0061 F 43 F LNU413 0.80 0.0059 C LNU506 0.77 0.0097 F 43 F LNU413 0.790.0062 A LNU342 0.70 0.0234 F 43 F LNU384 0.79 0.0061 E LNU383 0.880.0008 E 43 E LNU295 0.74 0.0345 C LNU384 0.70 0.0234 F 36 F LNU500 0.760.0108 E LNU506 0.74 0.0136 F 36 F LNU390 0.79 0.0061 F LNU442 0.730.0161 F 44 F LNU405 0.75 0.0127 F LNU390 0.76 0.0111 F 44 F LNU383 0.700.0341 F LNU405 0.85 0.0018 F 44 F LNU429 0.87 0.0024 F LNU430 0.810.0047 F 44 F LNU442 0.77 0.0144 F LNU500 0.80 0.0051 F 44 F LNU323 0.870.0023 F LNU500 0.72 0.0425 C 45 C LNU310 0.92 0.0005 F LNU442 0.760.0107 C 46 C LNU500 0.76 0.0176 F LNU461 0.72 0.0184 A 46 A LNU331 0.780.0130 E LNU336 0.75 0.0133 A 46 A LNU430 0.75 0.0206 E LNU356 0.700.0233 A 46 A LNU375 0.76 0.0112 D LNU310 0.85 0.0017 C 47 C LNU413 0.710.0208 D LNU506 0.89 0.0006 F 48 F LNU413 0.71 0.0214 B LNU506 0.760.0101 E 48 E LNU328 0.72 0.0186 B LNU455 0.82 0.0034 E 48 E LNU413 0.800.0056 F LNU331 0.75 0.0117 E 38 E LNU411 0.78 0.0073 F LNU383 0.720.0196 E 38 E LNU384 0.75 0.0124 E LNU454 0.79 0.0061 E 38 E LNU330 0.760.0103 E LNU455 0.73 0.0158 E 38 E LNU356 0.73 0.0161 E LNU442 0.780.0082 F 49 F LNU500 0.76 0.0108 E LNU323 0.75 0.0128 F 49 F LNU329 0.910.0003 E LNU429 0.79 0.0067 F 49 F LNU411 0.72 0.0178 E LNU430 0.860.0014 F 49 F LNU384 0.70 0.0239 D LNU462 0.70 0.0239 E 49 E LNU390 0.700.0234 B LNU295 0.73 0.0166 F 37 F LNU323 0.84 0.0023 F LNU323 0.880.0007 F 37 F LNU429 0.86 0.0016 F LNU383 0.72 0.0190 F 37 F LNU375 0.730.0157 F LNU429 0.92 0.0002 F 37 F LNU310 0.84 0.0021 F LNU375 0.730.0159 F 37 F LNU500 0.72 0.0198 F LNU310 0.88 0.0008 F 37 F LNU390 0.730.0163 D LNU331 0.81 0.0043 E 37 E LNU390 0.75 0.0131 B LNU430 0.750.0119 E 37 E LNU405 0.79 0.0069 B LNU451 0.75 0.0127 F 52 F LNU411 0.800.0051 B LNU326 0.71 0.0208 C 54 C LNU323 0.79 0.0071 F LNU442 0.780.0074 A 54 A LNU429 0.77 0.0087 F LNU326 0.80 0.0050 A 54 A LNU310 0.840.0025 F LNU489 0.84 0.0025 A 54 A LNU390 0.91 0.0002 D LNU489 0.770.0092 F 59 F LNU405 0.86 0.0013 B LNU442 0.84 0.0022 E 59 E LNU411 0.870.0010 B LNU454 0.83 0.0029 E 59 E LNU451 0.73 0.0172 D LNU489 0.820.0041 E 59 E LNU413 0.71 0.0224 D LNU357 0.71 0.0483 C 60 C LNU405 0.710.0220 F LNU331 0.83 0.0109 C 60 C LNU451 0.73 0.0167 D LNU310 0.730.0412 C 60 C LNU413 0.79 0.0070 D LNU455 0.75 0.0323 C 60 C LNU323 0.810.0043 F LNU329 0.84 0.0096 C 60 C LNU429 0.82 0.0038 F LNU295 0.830.0033 F 61 F LNU375 0.84 0.0024 F LNU429 0.73 0.0171 F 61 F LNU310 0.780.0072 F LNU310 0.82 0.0039 F 61 F LNU500 0.80 0.0060 F LNU295 0.730.0169 E 61 E LNU461 0.71 0.0334 C LNU454 0.71 0.0207 E 61 E LNU328 0.780.0133 A LNU442 0.72 0.0431 C 62 C LNU370 0.78 0.0083 A LNU455 0.870.0049 C 62 C LNU413 0.75 0.0132 A LNU329 0.77 0.0270 C 62 C LNU413 0.730.0172 F Table 48. “Corr. Set ID” - correlation set ID according to thecorrelated parameters Table above. “Exp. Set” = Expression set.

Correlation of early vigor traits across collection of Tomato ecotypesunder Low nitrogen, 300 mM NaCl, and normal growth conditions—Ten tomatohybrids were grown in 3 repetitive plots, each containing 17 plants, ata net house under semi-hydroponics conditions. Briefly, the growingprotocol was as follows: Tomato seeds were sown in trays filled with amix of vermiculite and peat in a 1:1 ratio. Following germination, thetrays were transferred to the high salinity solution (300 mM NaCl inaddition to the Full Hoagland solution), low nitrogen solution (theamount of total nitrogen was reduced in a 90% from the full Hoaglandsolution, final amount of 0.8 mM N) or at Normal growth solution (FullHoagland containing 8 mM N solution, at 28±2° C.). Plants were grown at28±2° C.

Full Hoagland solution consists of: KNO₃—0.808 grams/liter, MgSO₄—0.12grams/liter, KH₂PO₄—0.172 grams/liter and 0.01% (volume/volume) of‘Super coratin’ micro elements (Iron-EDDHA[ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]—40.5 grams/liter;Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1grams/liter), solution's pH should be 6.5—6.8].

Analyzed tomato tissues—All 10 selected Tomato varieties were sample pereach treatment. Two types of tissues [leaves and roots] were sampled andRNA was extracted as described above. For convenience, each micro-arrayexpression information tissue type has received a Set ID as summarizedin Table 49 below.

TABLE 49 Tomato transcriptom expression sets Expression Set Set IDLeaves at 300 mM NaCl A Leaves at Normal conditions B Leaves at LowNitrogen conditions C Roots at 100 mM NaCl D Roots at Normal conditionsE Roots at Low Nitrogen conditions F Table 49. Provided are the tomatotranscriptom experimental sets.

Tomato vigor related parameters—following 5 weeks of growing, plant wereharvested and analyzed for Leaf number, plant height, chlorophyll levels(SPAD units), different indices of nitrogen use efficiency (NUE) andplant biomass. Next, analyzed data was saved to text files and processedusing the JMP statistical analysis software (SAS institute). Dataparameters collected are summarized in Table 50, herein below.

TABLE 50 Tomato correlated parameters (vectors) Correlation Correlationset ID Leaf No NaCl [number] 1 Leaf No Normal [number] 2 Leaf No NUE[number] 3 Leaf No Ratio NaCl/Normal 4 Leaf No Ratio NaCl/NUE 5 Leafnumber ratio NUE/Normal 6 NUE roots (Root Biomass [Dw]/SPAD) Cold 7 NUEroots (Root Biomass [Dw]/SPAD) Low N 8 NUE roots (Root Biomass[Dw]/SPAD) NaCl 9 NUE roots (Root Biomass [Dw]/SPAD) Normal 10 NUE rootsLow N 11 NUE roots Normal 12 NUE shoots (shoot Biomass [Dw]/SPAD) Cold13 NUE shoots (shoot Biomass [Dw]/SPAD) Low N 14 NUE shoots (shootBiomass [Dw]/SPAD) NaCl 15 NUE shoots (shoot Biomass [Dw]/SPAD) Normal16 NUE shoots Low N 17 NUE shoots Normal 18 NUE total biomass (TotalBiomass [Dw]/SPAD) Cold 19 NUE total biomass (Total Biomass [Dw]/SPAD)Low N 20 NUE total biomass (Total Biomass [Dw]/SPAD) NaCl 21 NUE totalbiomass (Total Biomass [Dw]/SPAD) Normal 22 NUE total biomass Low N 23NUE total biomass Normal 24 Plant biomass NaCl [gr] 25 Plant height NaCl[cm] 26 Plant height Normal [cm] 27 Plant height NUE [cm] 28 PlantHeight Ratio NaCl/Normal 29 Plant Height Ratio NaCl/NUE 30 Plant HeightRatio NUE/Normal 31 Ratio Shoot Biomass/Root Biomass Normal 32 RatioShoot Biomass/Root Biomass NUE 33 Root Biomass reduction compared tonormal [%] Low N 34 Shoot Biomass reduction compared to normal [%] Low N35 SPAD Cold [SPAD unit] 36 SPAD NaCl [SPAD unit] 37 SPAD Normal [SPADunit] 38 SPAD NUE [SPAD unit] 39 SPAD NUE/Normal 40 Table 50. Providedare the tomato correlated parameters, NUE means nitrogen use efficiency

Experimental Results

10 different Tomato varieties were grown and characterized forparameters as described above. The average for each of the measuredparameter was calculated using the JMP software and values aresummarized in Tables 51-53 below. Subsequent correlation analysis wasconducted (Table 54). Follow, results were integrated to the database.

TABLE 51 Measured parameters in Tomato accessions under low nitrogenconditions Corr. Line ID 1139 2078 2958 5077 5080 5084 5085 5088 50895092 5113 28 36.8 39.9 34.4 47.0 46.4 45.4 47.7 39.3 41.8 41.0 27 45.347.8 40.8 55.3 56.2 48.7 55.8 37.4 49.6 46.3 39 34.6 24.9 28.6 31.6 29.731.8 30.3 30.3 31.3 28.8 6 0.85 0.90 0.98 1.09 0.88 1.02 0.87 1.06 0.911.12 31 0.81 0.83 0.84 0.85 0.83 0.93 0.85 1.05 0.84 0.88 40 1.01 0.981.02 1.00 0.98 0.98 0.93 1.05 1.01 0.99 3 5.6 6.2 7.2 6.8 5.6 6.6 5.15.9 5.6 6.3 14 0.004 0.004 0.003 0.007 0.005 0.005 0.012 0.007 0.0070.007 0.006 8 0.001 0.001 0.000 0.001 0.001 0.001 0.001 0.001 0.0010.001 0.001 20 0.005 0.005 0.003 0.008 0.005 0.006 0.013 0.008 0.0080.008 0.007 39 10.9 11.5 11.4 10.4 11.2 8.9 7.9 8.0 10.3 8.6 14.5 33 5.06.4 11.4 9.5 11.6 8.2 10.4 10.5 8.2 8.0 3.9 35 75.4 62.2 55.1 49.7 63.282.7 66.9 108.0 55.4 54.4 59.7 34 62.6 144.0 54.2 70.5 59.7 96.1 107.0112.0 81.6 32.2 87.5 17 35.4 38.4 24.1 65.0 46.7 46.7 120.0 60.1 66.356.5 60.3 11 7.0 7.7 2.5 7.0 5.0 8.0 15.1 9.0 8.8 7.3 15.9 23 58.5 69.763.8 69.3 71.1 60.5 73.9 68.8 66.7 70.8 49.7 Table 51.

TABLE 52 Measured parameters in Tomato accessions under normalconditions Corr. Line ID 1139 2078 2958 5077 5080 5084 5085 5088 50895092 5113 2 6.6 6.9 7.3 6.2 6.3 6.4 5.9 5.6 6.1 5.7 27 45.3 47.8 40.855.3 56.2 48.7 55.8 37.4 49.6 46.3 38 34.3 25.3 28.1 31.4 30.2 32.4 32.628.8 30.9 29.0 16 0.005 0.006 0.005 0.014 0.008 0.005 0.017 0.007 0.0110.012 0.009 10 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.001 0.0010.003 0.002 22 0.006 0.007 0.006 0.016 0.009 0.006 0.019 0.008 0.0120.014 0.011 38 9.3 10.2 8.9 8.4 9.8 8.6 6.6 7.0 8.7 7.4 9.4 32 5.4 12.710.0 15.4 8.8 7.5 12.6 8.0 14.3 4.8 6.3 18 4.7 6.2 4.4 13.1 7.4 5.7 17.95.6 12.0 10.4 10.1 12 1.1 0.5 0.5 1.0 0.8 0.8 0.9 0.8 1.1 2.3 1.8 24 7.59.1 8.6 8.9 7.2 7.9 9.1 7.9 8.6 8.7 6.2 Table 52.

TABLE 53 Measured parameters in Tomato accessions under salinityconditions Corr. Line ID 1139 2078 2958 5077 5080 5084 5085 5088 50895092 5113 1 3.6 3.9 5.0 4.0 3.6 4.4 3.2 3.7 4.0 4.3 26 5.6 6.5 8.5 8.68.9 7.6 8.6 5.6 5.8 9.4 25 0.36 0.44 0.26 0.71 0.46 0.54 0.66 0.40 0.520.45 4 0.54 0.57 0.68 0.64 0.56 0.68 0.54 0.67 0.65 0.75 5 0.64 0.630.69 0.59 0.64 0.67 0.62 0.63 0.72 0.68 29 0.12 0.14 0.21 0.15 0.16 0.160.15 0.15 0.12 0.20 30 0.15 0.16 0.25 0.18 0.19 0.17 0.18 0.14 0.14 0.2315 0.00051 0.00072 0.00067 0.00117 0.00172 0.00098 0.00117 0.000750.00101 0.00102 0.00069 37 11.4 10.4 11.6 10.8 10.8 7.0 9.2 8.5 10.4 8.812.4 9 0.00006 0.00005 0.00011 0.00010 0.00007 0.00009 0.00010 0.000080.00009 0.00005 21 0.00072 0.00063 0.00081 0.00142 0.00178 0.001070.00126 0.00083 0.00111 0.00069 Table 53.

TABLE 54 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under low nitrogen, normal or salinity stressconditions across Tomato accessions Gene Exp. Corr. Gene Exp. Corr. NameR P set ID ID Name R P set ID ID LNU329 0.7083 0.0493 E 28 LNU326 0.87880.0041 B 27 LNU302 0.7327 0.0387 E 28 LNU330 0.8425 0.0087 E 27 LNU3570.7723 0.0247 B 31 LNU413 0.7917 0.0192 E 27 LNU331 0.8767 0.0043 B 31LNU302 0.7866 0.0206 E 27 LNU383 0.8600 0.0062 B 31 LNU384 0.7754 0.0238C 2 LNU328 0.8777 0.0042 B 31 LNU342 0.9151 0.0014 C 2 LNU357 0.75360.0308 E 31 LNU329 0.7521 0.0314 C 2 LNU328 0.7973 0.0178 E 31 LNU3360.8290 0.0109 F 2 LNU310 0.7634 0.0275 E 31 LNU342 0.9078 0.0018 F 2LNU390 0.8277 0.0059 B 8 LNU328 0.7701 0.0254 F 2 LNU411 0.7071 0.0331 F12 LNU342 0.7150 0.0462 B 3 LNU413 0.7337 0.0245 F 24 LNU384 0.81770.0131 E 3 LNU413 0.7107 0.0482 D 9 LNU326 0.7485 0.0128 A 1 LNU4190.7467 0.0208 D 15 LNU411 0.7105 0.0213 A 1 LNU419 0.7120 0.0476 D 21LNU506 0.7282 0.0169 D 1 LNU429 0.7081 0.0328 E 11 LNU342 0.7964 0.0102C 38 LNU429 0.7655 0.0162 E 17 LNU405 0.7466 0.0208 C 38 LNU429 0.77250.0147 B 23 LNU342 0.8237 0.0064 F 38 LNU429 0.7482 0.0204 E 14 LNU2950.7157 0.0301 B 39 LNU429 0.7468 0.0208 E 20 LNU342 0.7705 0.0151 B 39LNU442 0.7235 0.0276 C 10 LNU342 0.7524 0.0193 E 39 LNU454 0.8033 0.0091B 11 LNU328 0.7810 0.0130 E 39 LNU454 0.7311 0.0252 C 16 LNU302 0.82530.0062 F 18 LNU454 0.8641 0.0027 B 17 LNU302 0.7142 0.0306 E 17 LNU4540.7053 0.0338 B 8 LNU328 0.7733 0.0145 F 11 LNU454 0.8078 0.0084 B 14LNU330 0.8985 0.0010 F 16 LNU454 0.8065 0.0086 B 20 LNU330 0.7329 0.0247E 14 LNU455 0.7856 0.0121 E 17 LNU357 0.7146 0.0305 B 8 LNU455 0.77100.0150 E 14 LNU370 0.7600 0.0175 D 15 LNU455 0.8144 0.0075 D 15 LNU3700.8050 0.0159 A 21 LNU455 0.7123 0.0313 F 22 LNU370 0.7489 0.0325 D 21LNU455 0.7665 0.0160 E 20 LNU375 0.7687 0.0155 F 24 LNU430 0.7661 0.0161C 32 LNU383 0.8420 0.0044 E 11 LNU461 0.7475 0.0206 F 32 LNU383 0.84890.0038 E 17 LNU375 0.7001 0.0357 F 32 LNU383 0.7579 0.0180 E 8 LNU3020.7956 0.0103 F 32 LNU383 0.8014 0.0094 E 14 LNU370 0.7836 0.0125 E 33LNU383 0.8080 0.0084 E 20 LNU328 0.7480 0.0328 B 40 LNU506 0.7019 0.0351B 34 LNU310 0.7821 0.0218 E 40 LNU468 0.7567 0.0183 B 34 LNU457 0.71040.0483 E 40 LNU357 0.7732 0.0145 B 34 LNU455 0.7766 0.0234 D 21 LNU3900.8615 0.0028 B 34 LNU461 0.7563 0.0184 E 23 LNU430 0.7132 0.0310 E 34LNU461 0.7072 0.0498 A 9 LNU357 0.8028 0.0092 B 35 LNU461 0.7008 0.0355F 16 LNU331 0.8319 0.0054 B 35 LNU468 0.7215 0.0282 B 11 LNU383 0.89010.0013 B 35 LNU468 0.7176 0.0295 E 11 LNU328 0.8573 0.0031 B 35 LNU4680.7581 0.0179 B 8 LNU331 0.7450 0.0213 E 35 LNU468 0.7499 0.0200 E 8LNU413 0.7913 0.0064 D 25 LNU489 0.8607 0.0029 C 10 LNU419 0.7032 0.0233D 25 LNU489 0.8498 0.0037 C 12 LNU383 0.8065 0.0048 A 26 LNU489 0.83790.0048 F 12 LNU384 0.7849 0.0072 D 26 LNU489 0.8969 0.0010 F 10 LNU3290.7535 0.0118 D 26 LNU489 0.7049 0.0340 B 8 LNU326 0.8788 0.0041 C 27LNU489 0.7166 0.0298 F 22 LNU330 0.8425 0.0087 F 27 LNU489 0.8254 0.0116A 21 LNU413 0.7917 0.0192 F 27 LNU489 0.8603 0.0061 D 21 LNU302 0.78660.0206 F 27 LNU506 0.7567 0.0183 E 14 LNU326 0.8727 0.0047 B 28 LNU4130.7216 0.0433 E 28 Table 54. “Corr. Set ID” - correlation set IDaccording to the correlated parameters Table above.

Example 11 Production of Barley Transcriptom and High ThroughputCorrelation Analysis Using 60K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparingbetween plant phenotype and gene expression level, the present inventorsutilized a Barley oligonucleotide micro-array, produced by AgilentTechnologies [Hypertext Transfer Protocol://World Wide Web (dot) chem.(dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. The arrayoligonucleotide represents about 60K Barley genes and transcripts. Inorder to define correlations between the levels of RNA expression andyield or vigor related parameters, various plant characteristics of 15different Barley accessions were analyzed. Among them, 10 accessionsencompassing the observed variance were selected for RNA expressionanalysis. The correlation between the RNA levels and the characterizedparameters was analyzed using Pearson correlation test [HypertextTransfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Barley tissues—Four tissues at different developmental stages[leaf, meristem, root tip and adventitious root], representing differentplant characteristics, were sampled and RNA was extracted as describedabove. Each micro-array expression information tissue type has receiveda Set ID as summarized in Table 55 below.

TABLE 55 Barley transcriptom expression sets Expression Set Set IDLeaf/drought/reproductive A Leaf/drought/vegetative B Leaf/low N/TP3 CLeaf/normal/TP3 D Root tip/low N/TP3 E Root tip/normal/TP3 F Roottip/drought/vegetative G Root tip/recovery drought/vegetative H Advroot/low N/TP3 I Adv root/normal/TP3 J Meristem/drought/vegetative K

Barley yield components and vigor related parameters assessment—15Barley accessions in 5 repetitive blocks, each containing 5 plants perpot were grown at net house. Three different treatments were applied:plants were regularly fertilized and watered during plant growth untilharvesting (as recommended for commercial growth) or under low Nitrogen(80% percent less Nitrogen) or drought stress. Plants were phenotyped ona daily basis following the parameters listed in Table 56 below. Harvestwas conducted while all the spikes were dry. All material was oven driedand the seeds were threshed manually from the spikes prior tomeasurement of the seed characteristics (weight and size) using scanningand image analysis. The image analysis system included a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37 (Java based image processing program, which wasdeveloped at the U.S. National Institutes of Health and freely availableon the internet [Hypertext Transfer Protocol://rsbweb (dot) nih (dot)gov/]. Next, analyzed data was saved to text files and processed usingthe JMP statistical analysis software (SAS institute).

Grains number—The total number of grains from all spikes that weremanually threshed was counted. No. of grains per plot were counted.

Grain weight (gr.)—At the end of the experiment all spikes of the potswere collected. The total grains from all spikes that were manuallythreshed were weight. The grain yield was calculated by per plot.

Spike length and width analysis—At the end of the experiment the lengthand width of five chosen spikes per plant were measured using measuringtape excluding the awns.

Spike number analysis—The spikes per plant were counted.

Plant height—Each of the plants was measured for its height usingmeasuring tape. Height was measured from ground level to top of thelongest spike excluding awns at two time points at the Vegetative growth(30 days after sowing) and at harvest.

Spike weight—The biomass and spikes weight of each plot was separated,measured and divided by the number of plants.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours at twotime points at the Vegetative growth (30 days after sowing) and atharvest.

Root dry weight=total weight of the root portion underground afterdrying at 70° C. in oven for 48 hours at harvest.

Root/Shoot Ratio—The Root/Shoot Ratio is calculated using Formula XV.

Root/Shoot Ratio=total weight of the root at harvest/total weight of thevegetative portion above ground at harvest.  Formula XV:

Total No. of tillers-all tillers were counted per plot at two timepoints at the Vegetative growth (30 days after sowing) and at harvest.

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed at time of flowering.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot.

Root FW (gr.), root length (cm) and No of lateral roots—3 plants perplot were selected for measurement of root weight, root length and forcounting the number of lateral roots formed.

Shoot FW—weight of 3 plants per plot were recorded at differenttime-points.

Relative water content—Fresh weight (FW) of three leaves from threeplants each from different seed ID was immediately recorded; then leaveswere soaked for 8 hours in distilled water at room temperature in thedark, and the turgid weight (TW) was recorded. Total dry weight (DW) wasrecorded after drying the leaves at 60° C. to a constant weight.Relative water content (RWC) is calculated according to Formula I above.

Harvest Index (for barley)—The harvest index is calculated using FormulaX above.

Relative growth rate: the relative growth rate (RGR) of Plant Height(Formula XI above), Spad (Formula XVI) and number of tillers (FormulaXVII) are calculated as follows:

Relative growth rate of SPAD=Regression coefficient of SPAD measurementsalong time course.  Formula XVI:

Relative growth rate of Number of tillers=Regression coefficient ofNumber of tillers along time course.  Formula XVII:

TABLE 56 Barley correlated parameters (vectors) Correlation setCorrelation ID Chlorophyll level 30DAG [SPAD] Drought 1 Chlorophylllevel at TP3 [SPAD] Low N 2 Chlorophyll level at TP3 [SPAD] Normal 3Grain yield per plant [gr.] Drought 4 Grain yield per plot [gr.] Low N 5Grain yield per plot [gr.] Normal 6 Grain yield per plot [gr.] Normal 7Grains per plant [number] Drought 8 Grains per plot [number] Low N 9Grains per plot [number] Normal 10 Harvest index [number] Drought 11Lateral roots per plant 30DAG [number] Drought 12 Lateral roots perplant at TP3 [number] Low N 13 Lateral roots per plant at TP3 [number]Normal 14 Leaf Area at TP4 [number] Low N 15 Leaf Area at TP4 [number]Normal 16 Leaf maximal length at TP4 [mm] Low N 17 Leaf maximal lengthat TP4 [mm] Normal 18 Leaf maximal width at TP4 [mm] Low N 19 Leafmaximal width at TP4 [mm]Normal 20 Number of leaves per plant at TP4[number] 21 Low N Number of leaves per plant at TP4 [number] 22 NormalPlant height per plant at TP3 [cm] Low N 23 Plant height per plot atharvest [cm] Drought 24 Plant height per plot at harvest [cm] Low N 25Plant height per plot at harvest [cm] Normal 26 Relative water content30DAG [percent] Drought 27 Root DW per plant at harvest [gr.]/Shoot DWper 28 plant at harvest [gr.] Drought Root DW per plant at harvest [gr.]Drought 29 Root FW per plant 30DAG [gr.] Drought 30 Root FW per plant atTP3 [gr.] Low N 31 Root FW per plant at TP3 [gr.] Normal 32 Root lengthper plant 30DAG [cm] Drought 33 Root length per plant at TP3 [cm] Low N34 Root length per plant at TP3 [cm]Normal 35 Shoot DW at harvest perplant [gr.] Drought 36 Shoot FW per plant at 30DAG [gr.] Drought 37Shoot FW per plant at TP3 [gr.] Low N 38 Shoot FW per plant at TP3 [gr.]Normal 39 Spike length [cm] Drought 40 Spike length [cm] Low N 41 Spikelength [cm] Normal 42 Spike width [mm] Drought 43 Spike width [mm] Low N44 Spike width [mm] Normal 45 Spikes per plant [number] Drought 46Spikes per plot [number] Low N 47 Spikes per plot [number] Normal 48Spikes weight per plant [gr.] Drought 49 Spikes yield per plot [gr.] LowN 50 Spikes yield per plot [gr.] Normal 51 Tillers per plant at TP3[number] Low N 52 Tillers per plant at harvest [number] Drought 53Tillers per plot at harvest [number] Low N 54 Tillers per plot atharvest [number] Normal 55 Tillers per plant at TP3 [number] Normal 56Table 56. Provided are the barley correlated parameters, TP means timepoint, DW-dry weight, FW-fresh weight and Low N-Low Nitrogen.

Experimental Results

15 different Barley accessions were grown and characterized fordifferent parameters as described above. The average for each of themeasured parameter was calculated using the JMP software and values aresummarized in Tables 57-60 below. Subsequent correlation analysisbetween the various transcriptom sets and the average parameters (Table61) was conducted. Follow, results were integrated to the database.

TABLE 57 Measured parameters of correlation Ids in Barley accessionsunder low Nitrogen conditions Corr. Line ID 2 4 6 9 11 13 15 31 50 53 2110.0 8.6 7.5 7.5 8.0 8.0 10.0 11.5 8.5 6.3 17 152 124 112 124 108 103135 149 142 95 19 5.2 5.3 5.1 5.2 5.2 5.3 5.1 5.3 5.3 5.1 13 6.3 6.7 4.35.7 6.0 5.0 7.3 6.0 6.0 4.7 25 65.8 53.8 61.4 81.8 82.0 41.0 44.6 47.859.4 56.4 23 22.5 19.7 17.3 19.2 18.8 16.3 19.2 18.2 26.0 19.8 31 0.880.43 0.12 0.30 0.23 0.38 0.55 0.50 0.40 0.32 34 22.2 30.5 22.0 23.8 21.724.7 24.5 23.0 21.7 22.8 9 106.0 219.0 88.2 202.0 165.0 230.0 125.0223.0 134.0 143.0 5 6.0 7.4 3.3 7.8 7.3 9.8 6.3 9.7 5.1 5.8 6 30.3 37.010.8 35.4 19.8 46.4 38.3 54.1 22.6 42.0 38 0.78 0.45 0.33 0.50 0.43 0.430.62 0.53 0.58 0.43 2 26.6 25.4 26.5 25.0 23.3 24.0 26.1 23.2 23.9 24.241 90.2 20.4 16.3 18.8 19.6 15.2 16.6 16.4 19.3 18.8 50 11.3 12.2 9.212.2 13.4 13.7 10.6 15.1 11.6 10.9 44 9.6 7.1 9.4 10.0 8.1 8.0 9.4 7.24.9 8.5 15 67.8 52.4 51.5 68.0 46.3 39.4 57.9 64.2 57.1 46.2 47 7.8 15.011.6 5.4 9.0 12.2 8.4 14.5 25.0 7.0 54 12.5 21.2 16.0 6.8 14.6 16.2 14.018.8 20.8 11.0 Table 57.

TABLE 58 Measured parameters of correlation Ids in Barley accessionsunder normal conditions Corr. Line ID 2 4 6 9 11 13 15 31 50 53 6 30.337.0 10.8 35.4 19.8 46.4 38.3 54.1 22.6 42.0 10 621 903 242 984 510 1090768 1070 582 950 26 72.0 65.8 67.4 91.6 84.0 64.7 66.2 56.6 82.0 62.8 4834.2 49.8 36.0 19.3 32.0 41.5 38.0 45.6 71.4 28.0 42 17.2 20.3 18.3 16.519.2 16.5 16.1 19.1 20.4 21.7 45 10.5 7.4 8.3 10.2 9.1 9.5 10.3 8.8 6.610.4 51 60.8 62.7 34.9 55.9 39.4 69.4 59.7 79.1 50.3 60.0 55 34.6 49.240.0 27.5 41.6 46.7 38.8 48.6 48.8 29.0 35 27.2 24.0 21.8 21.5 15.0 21.315.2 16.0 20.3 13.5 14 10.7 9.7 8.3 10.0 8.7 7.0 9.7 9.7 9.7 8.7 32 0.620.35 0.25 0.23 0.27 0.27 0.27 0.27 0.35 0.32 56 2.3 2.3 1.0 1.3 2.0 2.01.7 3.3 2.3 1.3 3 34.2 37.0 35.2 35.0 41.4 39.1 36.8 42.8 33.7 36.9 3915.6 2.6 1.3 2.2 1.9 2.2 1.8 3.0 3.0 1.8 22 23.2 22.2 22.7 17.3 18.224.2 22.0 28.3 25.5 19.0 16 313 259 273 299 199 294 296 309 276 291 204.6 5.8 5.8 5.8 5.5 5.8 6.0 5.3 6.0 5.4 18 535 479 499 384 348 502 470551 594 399 Table 58.

TABLE 59 Measured parameters of correlation Ids in Barley accessionsunder drought conditions Corr. Line ID 2 4 6 8 9 10 11 13 11 0.69 0.600.29 0.44 0.78 0.47 0.66 0.53 27 69.8 87.4 58.3 80.6 73.1 80.6 53.4 55.928 0.02 0.01 0.02 0.01 0.01 0.01 0.01 0.01 8 252 348 72 160 377 170 268111 4 7.75 8.50 2.05 5.38 11.00 5.55 9.80 3.55 24 48.0 40.8 47.4 64.852.6 46.0 52.8 35.0 46 3.43 8.55 3.05 4.07 3.72 4.20 4.36 7.60 40 15.616.0 14.2 14.8 16.5 16.7 16.8 13.3 43 7.62 6.06 7.84 7.81 8.35 8.64 9.077.82 49 15.0 22.0 11.7 18.8 21.0 17.7 24.2 18.2 36 3.55 5.67 5.12 6.863.11 6.15 5.05 3.20 29 70.7 66.2 117.0 84.1 37.5 77.5 60.2 27.1 33 18.321.7 17.0 15.2 27.0 21.7 20.3 22.0 12 6.67 6.00 6.33 7.00 7.00 8.33 8.677.33 30 1.68 1.45 0.58 0.63 1.07 2.07 1.48 1.12 53 8.78 13.90 8.45 9.155.12 11.70 9.04 10.90 1 39.7 42.1 42.4 42.3 36.8 41.3 33.6 36.6 37 1.221.88 0.90 0.90 1.43 1.90 1.52 1.17 Table 59.

TABLE 60 Additional measured parameters of correlation IDs in Barleyaccessions under drought conditions Corr. Line ID 15 31 38 50 53 93 13s11 0.53 0.69 0.75 0.81 0.87 0.41 0.69 27 43.2 45.5 76.5 28 0.03 0.010.01 0.01 0.02 0.03 0.01 8 154 288 274 358 521 105 205 4 5.28 9.92 10.2014.00 17.50 2.56 7.20 24 45.20 37.70 41.20 49.90 43.00 32.00 38.00 464.92 6.90 5.80 9.67 5.42 3.21 8.44 40 14.20 15.70 17.50 18.30 17.4012.70 13.50 43 8.74 6.98 8.05 6.72 9.55 5.47 7.32 49 19.50 23.40 28.2033.00 34.80 9.88 18.00 36 4.76 4.52 3.38 3.31 2.65 3.74 3.28 29 117 3726 22 41 99 19 33 20.7 21.0 20.3 19.7 16.7 15.0 24.0 12 6.67 7.67 6.678.67 7.67 6.67 7.67 30 1.67 1.62 0.85 1.38 0.82 0.70 1.87 53 10.30 13.007.44 11.00 6.78 16.10 10.20 1 45.10 38.30 36.20 31.80 33.50 40.60 40.5037 1.90 1.75 1.58 1.73 1.00 0.83 1.95 Table 60.

TABLE 61 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under low nitrogen, normal or drought stressconditions across Barley accessions Gene Exp. Corr. Gene Exp. Corr. NameR P Set ID ID Name R P set ID ID LNU488 0.72 0.0462 D 10 LNU322 0.890.0032 B 36 LNU407 0.74 0.0371 D 10 LNU436 0.85 0.0037 K 36 LNU507 0.740.0349 D 10 LNU425 0.76 0.0164 K 36 LNU502 0.76 0.0174 I 9 LNU436 0.860.0056 G 36 LNU409 0.72 0.0293 I 9 LNU435 0.81 0.0085 K 37 LNU488 0.860.0014 E 9 LNU305 0.70 0.0342 H 37 LNU436 0.90 0.0004 E 9 LNU407 0.820.0236 A 8 LNU437 0.83 0.0030 E 9 LNU502 0.76 0.0491 A 8 LNU507 0.830.0030 E 9 LNU466 0.73 0.0378 G 8 LNU425 0.82 0.0067 C 13 LNU448 0.720.0428 G 8 LNU448 0.83 0.0028 E 13 LNU448 0.78 0.0129 H 8 LNU487 0.880.0008 E 13 LNU487 0.76 0.0488 A 4 LNU425 0.77 0.0251 D 16 LNU502 0.840.0176 A 4 LNU467 0.79 0.0207 D 16 LNU297 0.71 0.0312 K 4 LNU472 0.820.0063 C 17 LNU466 0.71 0.0477 G 4 LNU435 0.81 0.0155 D 18 LNU448 0.710.0306 H 4 LNU407 0.87 0.0026 J 3 LNU437 0.73 0.0262 H 4 LNU466 0.800.0099 J 3 LNU502 0.85 0.0165 A 11 LNU425 0.82 0.0072 J 3 LNU466 0.720.0457 G 11 LNU507 0.75 0.0328 D 3 LNU437 0.73 0.0269 H 11 LNU407 0.720.0420 F 3 LNU391 0.76 0.0483 A 12 LNU466 0.73 0.0408 F 3 LNU499 0.810.0286 A 24 LNU488 0.83 0.0058 I 19 LNU322 0.77 0.0426 A 24 LNU391 0.750.0206 I 19 LNU322 0.78 0.0235 B 24 LNU408 0.83 0.0054 I 19 LNU436 0.850.0080 B 24 LNU488 0.85 0.0040 C 19 LNU437 0.76 0.0274 B 24 LNU407 0.780.0130 C 19 LNU507 0.79 0.0199 B 24 LNU436 0.72 0.0284 C 19 LNU472 0.740.0217 K 24 LNU425 0.78 0.0081 E 19 LNU409 0.72 0.0300 K 24 LNU507 0.700.0340 I 21 LNU499 0.81 0.0154 G 24 LNU425 0.76 0.0175 C 21 LNU436 0.770.0262 G 24 LNU425 0.77 0.0089 E 21 LNU437 0.74 0.0352 G 24 LNU435 0.760.0182 J 22 LNU409 0.74 0.0356 G 24 LNU435 0.88 0.0036 D 22 LNU407 0.900.0061 A 28 LNU456 0.80 0.0173 D 22 LNU425 0.86 0.0129 A 28 LNU436 0.710.0468 F 22 LNU447 0.83 0.0199 A 28 LNU322 0.77 0.0148 I 23 LNU409 0.850.0163 A 28 LNU436 0.75 0.0187 C 23 LNU425 0.84 0.0083 B 28 LNU472 0.730.0260 C 23 LNU456 0.80 0.0165 B 28 LNU425 0.86 0.0014 E 23 LNU408 0.730.0412 G 28 LNU437 0.74 0.0235 C 25 LNU425 0.84 0.0099 G 28 LNU467 0.820.0131 D 26 LNU322 0.82 0.0069 H 28 LNU322 0.72 0.0445 F 26 LNU407 0.800.0313 H 27 LNU436 0.79 0.0206 F 26 LNU448 0.88 0.0093 A 29 LNU436 0.790.0109 I 31 LNU305 0.76 0.0293 B 29 LNU487 0.76 0.0176 I 31 LNU322 0.760.0284 B 29 LNU438 0.80 0.0097 C 31 LNU456 0.79 0.0195 B 29 LNU425 0.860.0030 C 31 LNU507 0.77 0.0244 B 29 LNU472 0.74 0.0216 C 31 LNU409 0.870.0049 B 29 LNU502 0.72 0.0303 C 31 LNU408 0.73 0.0389 G 29 LNU437 0.780.0135 J 32 LNU438 0.85 0.0075 G 29 LNU499 0.79 0.0186 D 32 LNU456 0.720.0456 G 29 LNU425 0.72 0.0428 F 32 LNU409 0.76 0.0290 G 29 LNU466 0.790.0067 E 34 LNU407 0.71 0.0334 H 29 LNU436 0.79 0.0063 E 34 LNU438 0.740.0361 B 30 LNU437 0.76 0.0170 J 35 LNU305 0.70 0.0347 H 30 LNU437 0.740.0364 F 35 LNU438 0.73 0.0411 B 33 LNU436 0.76 0.0184 I 38 LNU447 0.730.0245 H 33 LNU425 0.71 0.0324 I 38 LNU305 0.74 0.0364 G 40 LNU472 0.800.0096 I 38 LNU305 0.78 0.0376 A 46 LNU438 0.79 0.0107 C 38 LNU499 0.820.0129 B 46 LNU425 0.76 0.0185 C 38 LNU467 0.73 0.0415 B 46 LNU472 0.760.0176 C 38 LNU502 0.80 0.0180 B 46 LNU502 0.79 0.0121 C 38 LNU472 0.870.0022 H 46 LNU437 0.82 0.0068 J 39 LNU502 0.88 0.0092 A 49 LNU499 0.830.0105 D 39 LNU502 0.80 0.0167 B 49 LNU425 0.72 0.0294 I 41 LNU297 0.820.0074 K 49 LNU438 0.81 0.0082 C 41 LNU437 0.71 0.0311 H 49 LNU425 0.780.0139 C 41 LNU466 0.88 0.0090 A 43 LNU487 0.70 0.0351 C 41 LNU425 0.820.0228 A 43 LNU502 0.86 0.0027 C 41 LNU447 0.80 0.0313 A 43 LNU448 0.780.0213 D 42 LNU409 0.80 0.0296 A 43 LNU487 0.72 0.0455 D 42 LNU305 0.770.0244 B 43 LNU305 0.77 0.0143 I 44 LNU507 0.74 0.0356 B 43 LNU487 0.760.0185 I 44 LNU391 0.72 0.0302 K 43 LNU499 0.79 0.0203 D 45 LNU488 0.830.0110 B 53 LNU425 0.72 0.0437 D 45 LNU435 0.85 0.0036 K 53 LNU436 0.810.0080 C 47 LNU467 0.84 0.0050 K 53 LNU322 0.87 0.0024 J 48 LNU467 0.790.0193 G 53 LNU488 0.76 0.0284 D 48 LNU435 0.73 0.0271 H 53 LNU407 0.770.0269 D 48 LNU456 0.83 0.0053 C 2 LNU425 0.77 0.0256 D 48 LNU407 0.850.0038 I 5 LNU437 0.86 0.0067 D 48 LNU435 0.80 0.0099 I 5 LNU437 0.790.0198 F 48 LNU408 0.80 0.0100 I 5 LNU436 0.72 0.0289 J 48 LNU467 0.860.0033 I 5 LNU487 0.71 0.0498 D 48 LNU502 0.86 0.0027 I 5 LNU435 0.700.0350 I 50 LNU409 0.78 0.0134 I 5 LNU408 0.76 0.0177 I 50 LNU466 0.710.0315 C 5 LNU438 0.72 0.0302 I 50 LNU391 0.73 0.0250 C 5 LNU467 0.790.0106 I 50 LNU507 0.71 0.0308 C 5 LNU507 0.73 0.0243 I 50 LNU322 0.770.0095 E 5 LNU466 0.78 0.0123 C 50 LNU436 0.81 0.0046 E 5 LNU391 0.810.0081 C 50 LNU437 0.76 0.0107 E 5 LNU437 0.72 0.0191 E 50 LNU502 0.720.0186 E 5 LNU408 0.75 0.0201 J 51 LNU408 0.71 0.0327 J 6 LNU507 0.730.0409 D 51 LNU507 0.77 0.0251 D 6 LNU488 0.73 0.0387 F 51 LNU407 0.840.0046 I 9 LNU488 0.72 0.0284 I 52 LNU435 0.78 0.0134 I 9 LNU467 0.730.0269 J 56 LNU408 0.88 0.0016 I 9 LNU488 0.71 0.0484 D 56 LNU438 0.860.0030 I 9 LNU407 0.72 0.0451 D 56 LNU467 0.79 0.0114 I 9 LNU437 0.720.0452 D 56 LNU408 0.81 0.0082 J 10 LNU436 0.74 0.0343 F 56 LNU438 0.720.0271 J 10 Table 61.

Example 12 Production of Maize Transcriptom and High ThroughputCorrelation Analysis with Yield and NUE Related Parameters Using 44KMaize Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis between plantphenotype and gene expression level, the present inventors utilized amaize oligonucleotide micro-array, produced by Agilent Technologies[Hypertext Transfer Protocol://World Wide Web (dot) chem. (dot) agilent(dot) com/Scripts/PDS (dot) asp?1Page=50879]. The array oligonucleotiderepresents about 44,000 maize genes and transcripts.

Correlation of Maize Hybrids Across Ecotypes Grown Under Low NitrogenConditions

Experimental Procedures

12 Maize hybrids were grown in 3 repetitive plots, in field. Maize seedswere planted and plants were grown in the field using commercialfertilization and irrigation protocols. In order to define correlationsbetween the levels of RNA expression with NUE and yield components orvigor related parameters, the 12 different maize hybrids were analyzed.Among them, 11 hybrids encompassing the observed variance were selectedfor RNA expression analysis. The correlation between the RNA levels andthe characterized parameters was analyzed using Pearson correlation test[Hypertext Transfer Protocol://World Wide Web (dot) davidmlane (dot)com/hyperstat/A34739 (dot) html].

Analyzed Maize tissues—All 10 selected maize hybrids were sample pereach treatment. Plant tissues [flag leaf, flower meristem, grain, earand internode] growing under Normal conditions were sampled and RNA wasextracted as described above. Each micro-array expression informationtissue type has received a Set ID as summarized in Table 62 below.

TABLE 62 Maize transcriptom expression sets Expression Set Set ID Maizefield/Low/N/Ear/TP5 A Maize field/Low/N/Ear/TP6 B Maizefield/Low/N/Internodes/TP2 C Maize field/Low/N/Internodes/TP5 D Maizefield/Low/N/Leaf/TP5 E Maize field/Low/N/Leaf/TP6 F Maizefield/Normal/Ear/R1-R2 G Maize field/Normal/Grain/Distal/R4-R5 H Maizefield/Normal/Internode/R3-R4 J Maize field/Normal/Internode/V6-V8 KMaize field/Normal/Leaf/R1-R2 L Maize field/Normal/Leaf/V6-V8 M Maizefield/Low/N/Internodes/TP6 N Table 62: Provided are the maizetranscriptom expression sets. Leaf = the leaf below the main ear; Flowermeristem = Apical meristem following male flower initiation; Ear = thefemale flower at the anthesis day. Grain Distal = maize developinggrains from the cob extreme area, Grain Basal = maize developing grainsfrom the cob basal area; Internodes = internodes located above and belowthe main ear in the plant.

The following parameters were collected using digital imaging system:

Grain Area (cm²)—At the end of the growing period the grains wereseparated from the ear. A sample of ˜200 grains were weighted,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.

Grain Length and Grain width (cm)—At the end of the growing period thegrains were separated from the ear. A sample of ˜200 grains wereweighted, photographed and images were processed using the belowdescribed image processing system. The sum of grain lengths/or width(longest axis) was measured from those images and was divided by thenumber of grains.

Ear Area (cm²)—At the end of the growing period 5 ears were,photographed and images were processed using the below described imageprocessing system. The Ear area was measured from those images and wasdivided by the number of Ears.

Ear Length and Ear Width (cm) At the end of the growing period 5 earswere, photographed and images were processed using the below describedimage processing system. The Ear length and width (longest axis) wasmeasured from those images and was divided by the number of ears.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/. Images were captured in resolution of 10 Mega Pixels(3888×2592 pixels) and stored in a low compression JPEG (JointPhotographic Experts Group standard) format. Next, image processingoutput data for seed area and seed length was saved to text files andanalyzed using the JMP statistical analysis software (SAS institute).

Additional parameters were collected either by sampling 6 plants perplot or by measuring the parameter across all the plants within theplot.

Normalized Grain Weight per plant (gr.)—At the end of the experiment allears from plots within blocks A-C were collected. Six ears wereseparately threshed and grains were weighted, all additional ears werethreshed together and weighted as well. The average grain weight per earwas calculated by dividing the total grain weight by number of totalears per plot (based on plot). In case of 6 ears, the total grainsweight of 6 ears was divided by 6.

Ear FW (gr.)—At the end of the experiment (when ears were harvested)total and 6 selected ears per plots within blocks A-C were collectedseparately. The plants with (total and 6) were weighted (gr.) separatelyand the average ear per plant was calculated for total (Ear FW per plot)and for 6 (Ear FW per plant).

Plant height and Ear height—Plants were characterized for height atharvesting. In each measure, 6 plants were measured for their heightusing a measuring tape. Height was measured from ground level to top ofthe plant below the tassel. Ear height was measured from the groundlevel to the place were the main ear is located.

Leaf number per plant—Plants were characterized for leaf number duringgrowing period at 5 time points. In each measure, plants were measuredfor their leaf number by counting all the leaves of 3 selected plantsper plot.

Relative Growth Rate was calculated using Formulas XI and XII (describedabove).

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed at early stages of grainfilling (R1-R2) and late stage of grain filling (R3-R4). SPAD meterreadings were done on young fully developed leaf. Three measurements perleaf were taken per plot. Data were taken after 46 and 54 days aftersowing (DPS).

Dry weight per plant—At the end of the experiment (when Inflorescencewere dry) all vegetative material from plots within blocks A-C werecollected.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours;

Harvest Index (HI) (Maize)—The harvest index was calculated usingFormula XIV.

Percent Filled Ear [%]—it was calculated as the percentage of the Eararea with grains out of the total ear.

Cob diameter [cm]—The diameter of the cob without grains was measuredusing a ruler.

Kernel Row Number per Ear—The number of rows in each ear was counted.

Experimental Results

11 different maize hybrids were grown and characterized for differentparameters: The average for each of the measured parameter wascalculated using the JMP software (Tables 63-65) and a subsequentcorrelation analysis was performed (Tables 66-67). Results were thenintegrated to the database.

TABLE 63 Maize correlated parameters (vectors) Correlation setCorrelation ID Ear Length [cm] Low N 1 Ear Length [cm] Normal 2 EarLength of filled area [cm] Low N 3 Ear Length of filled area [cm] Normal4 Ear width [mm] Low N 5 Ear width [mm] Normal 6 Ears weight per plot[kg] Low N 7 Ears weight per plot [kg] Normal 8 Final Leaf Area [number]Low N 9 Final Leaf Area [number] Normal 10 Final Leaf Number [number]Low N 11 Final Leaf Number [number] Normal 12 Final Main Ear Height [cm]Low N 13 Final Main Ear Height [cm] Normal 14 Final Plant DW [kg] Low N15 Final Plant DW [kg] Normal 16 Final Plant Height [cm] Low N 17 FinalPlant Height [cm] Normal 18 No of rows per ear [number] Low N 19 No ofrows per ear [number] Normal 20 NUE at early grain filling [R1-R2] yieldkg/N 21 in plant per SPAD Low N NUE at early grain filling [R1-R2] yieldkg/N 22 in plant per SPAD Normal NUE at grain filling [R3-R4] yield kg/Nin 23 plant per SPAD Low N NUE at grain filling [R3-R4] yield kg/N in 24plant per SPAD Normal NUE yield kg/N applied in soil kg Low N 25 NUEyield kg/N applied in soil kg Normal 26 NUpE [biomass/N applied] Low N27 NUpE [biomass/N applied] Normal 28 Seed yield per dunam [kg] Low N 29Seed yield per dunam [kg] Normal 30 seed yield per plant [kg] Normal 31seed yield per plant [kg] Low N 32 SPAD at R1-R2 [number] Low N 33 SPADat R1-R2 [number] Normal 34 SPAD at R3-R4 [number] Low N 35 SPAD atR3-R4 [number] Normal 36 Stalk width at TP5 Normal 37 Stalk width at TP5Low N 38 Yield/LAI Low N 39 Yield/LAI Normal 40 Yield/stalk width Normal41 Yield/stalk width Low N 42 Table 63. SPAD at R1-R2 and SPAD R3-R4:Chlorophyl leve after early and late stages of grain filling,NUE-nitrogen use efficiency, NUpE-nitrogen uptake efficiency, LAI-leafarea, Low N-Low Nitrogen.

TABLE 64 Measured parameters in Maize accessions under normal conditionsCorr. Line ID 1 2 3 4 5 6 7 8 9 10 11 16 1.27 1.30 1.33 1.50 1.30 1.581.42 1.37 1.70 11.40 0.42 8 8.94 7.02 7.53 7.99 8.48 5.63 6.10 6.66 8.218.40 1.88 31 0.17 0.14 0.15 0.16 0.15 0.12 0.12 0.13 0.15 0.17 0.04 301340 1090 1200 1270 1200 937 986 1050 1230 1370 301 18 273 260 288 238287 225 264 252 279 278 164 14 130.0 122.0 128.0 113.0 135.0 94.3 121.0108.0 140.0 112.0 60.4 12 11.8 11.1 13.3 11.8 11.9 12.3 12.4 12.2 11.712.6 9.3 37 2.9 2.6 2.7 2.9 2.7 2.6 2.9 2.7 2.7 2.8 2.3 2 19.9 20.2 18.119.9 19.5 17.7 17.7 17.3 17.5 20.5 19.9 6 51.1 46.3 45.9 47.6 51.4 47.447.3 46.8 48.3 49.3 41.8 4 16.2 17.5 17.7 18.4 15.7 14.7 12.9 14.0 12.318.8 16.1 20 16.1 14.7 15.4 15.9 16.2 15.2 16.0 14.8 17.7 15.4 14.3 3456.9 57.2 59.3 61.6 58.6 61.2 60.2 61.1 57.5 62.2 52.0 36 59.9 60.9 56.958.7 58.7 63.2 59.8 62.4 57.2 61.9 49.3 26 4.5 3.6 4.0 4.2 4.0 3.1 3.33.5 4.1 4.6 1.0 24 25.0 17.8 20.3 20.0 19.0 13.9 16.2 17.2 21.5 21.0 5.522 23.4 19.1 20.3 20.7 20.5 15.4 16.4 17.2 21.0 22.0 5.7 41 457 412 443439 447 357 337 386 472 482 140 28 0.008 0.009 0.009 0.010 0.009 0.0110.009 0.009 0.004 0.076 0.003 10 3.2 4.0 3.3 4.0 3.9 4.2 4.0 4.3 4.3 2.940 426 313 307 362 314 225 266 262 482 Table 64. Provided are the valuesof each of the parameters (as described above) measured in maizeaccessions (Seed ID) under regular growth conditions. Growth conditionsare specified in the experimental procedure section.

TABLE 65 Additional measured parameters in Maize accessions under lowNitrogen conditions Corr. Line ID 1 2 3 4 5 6 7 8 9 10 11 15 1.59 1.431.53 1.95 1.48 1.60 1.58 1.28 1.51 1.52 0.43 7 6.61 7.97 9.63 9.22 7.637.21 7.92 29.00 7.80 9.78 2.41 32 0.14 0.16 0.19 0.19 0.14 0.15 0.150.16 0.14 0.20 0.05 29 1080 1260 1550 1500 1140 1160 1210 1250 1150 1590383 17 306 271 291 252 260 227 272 249 279 270 171 13 158 136 128 133138 100 130 115 144 114 62 11 15.0 11.6 13.5 11.6 11.8 11.9 12.6 11.712.4 13.2 9.3 38 2.8 2.4 2.7 2.8 2.7 2.6 3.0 2.6 2.7 2.8 2.3 1 20.6 21.020.2 20.1 20.1 18.5 19.1 18.2 20.1 21.2 17.8 5 46.7 48.2 48.3 49.9 52.947.4 49.6 48.6 52.4 50.0 42.6 3 18.4 18.4 19.8 18.8 16.2 16.0 15.3 15.716.8 19.6 14.1 19 14.2 15.2 15.0 15.7 16.0 15.9 15.6 14.5 16.4 15.7 14.433 60.2 57.9 58.8 59.5 58.5 64.0 56.4 60.0 58.3 61.7 53.1 35 59.3 57.658.4 59.2 58.2 62.7 61.0 59.9 57.5 61.9 49.6 25 7.2 8.4 10.3 10.0 7.67.7 8.1 8.3 7.6 10.6 2.6 23 18.4 21.9 26.5 25.3 19.7 18.5 19.8 20.9 19.925.9 7.7 21 18.0 21.8 26.3 25.1 19.5 18.0 21.4 20.8 19.7 25.7 7.2 42 417528 583 541 428 444 407 477 446 562 168 27 0.011 0.010 0.010 0.013 0.0100.011 0.011 0.009 0.010 0.010 0.003 9 2.92 3.15 3.33 2.87 2.79 3.76 3.505.02 3.16 39 342 408 465 522 440 313 346 288 501 Table 65. Provided arethe values of each of the parameters (as described above) measured inmaize accessions (Seed ID) under regular growth conditions. Growthconditions are specified in the experimental procedure section.

TABLE 66 Correlation between the expression level of selected LNU genesof some embodiments of the invention in various tissues and thephenotypic performance under normal conditions across maize accessionsGene Exp. set Corr. Gene Exp. Corr. Name R P ID ID Name R P set ID IDLNU469 0.86 0.0135 A 21 LNU476 0.90 0.0060 G 41 LNU469 0.76 0.0289 E 21LNU476 0.72 0.0191 M 41 LNU476 0.90 0.0054 G 22 LNU519 0.78 0.0225 B 40LNU476 0.74 0.0152 M 22 LNU519 0.76 0.0291 H 41 LNU519 0.79 0.0205 H 22LNU519 0.83 0.0220 I 41 LNU519 0.79 0.0337 I 22 LNU299 0.73 0.0248 N 1LNU519 0.82 0.0120 B 21 LNU299 0.83 0.0102 F 1 LNU299 0.90 0.0065 A 23LNU311 0.82 0.0244 A 1 LNU299 0.80 0.0175 B 23 LNU317 0.82 0.0250 A 1LNU299 0.87 0.0011 C 23 LNU348 0.88 0.0019 K 2 LNU299 0.91 0.0020 F 23LNU394 0.79 0.0202 E 1 LNU300 0.77 0.0433 A 23 LNU394 0.88 0.0039 B 1LNU300 0.80 0.0172 B 23 LNU418 0.74 0.0343 H 2 LNU300 0.72 0.0428 F 23LNU418 0.87 0.0103 A 1 LNU301 0.78 0.0381 A 23 LNU460 0.89 0.0030 F 1LNU301 0.79 0.0202 B 23 LNU469 0.85 0.0143 A 1 LNU307 0.80 0.0058 C 23LNU469 0.79 0.0202 E 1 LNU307 0.79 0.0116 N 23 LNU476 0.85 0.0073 E 1LNU307 0.83 0.0103 E 23 LNU519 0.72 0.0420 J 2 LNU307 0.72 0.0437 F 23LNU299 0.81 0.0149 F 3 LNU339 0.89 0.0074 A 23 LNU317 0.88 0.0097 A 3LNU339 0.74 0.0351 E 23 LNU339 0.89 0.0070 A 3 LNU348 0.91 0.0018 F 23LNU348 0.76 0.0296 F 3 LNU360 0.80 0.0166 E 23 LNU376 0.71 0.0486 F 3LNU361 0.78 0.0387 A 23 LNU394 0.75 0.0129 M 4 LNU361 0.72 0.0438 E 23LNU394 0.82 0.0130 E 3 LNU371 0.80 0.0300 A 23 LNU394 0.84 0.0181 A 3LNU376 0.75 0.0316 F 23 LNU394 0.76 0.0300 F 3 LNU394 0.74 0.0348 E 23LNU394 0.86 0.0065 B 3 LNU394 0.76 0.0459 A 23 LNU418 0.82 0.0251 A 3LNU394 0.84 0.0085 B 23 LNU460 0.78 0.0220 F 3 LNU459 0.82 0.0124 F 23LNU469 0.88 0.0086 G 4 LNU469 0.84 0.0180 A 23 LNU469 0.71 0.0313 K 4LNU469 0.77 0.0265 E 23 LNU469 0.79 0.0337 L 4 LNU476 0.71 0.0478 F 23LNU469 0.79 0.0353 A 3 LNU519 0.78 0.0215 B 23 LNU469 0.72 0.0436 F 3LNU300 0.90 0.0051 G 24 LNU519 0.70 0.0353 K 4 LNU307 0.88 0.0089 G 24LNU299 0.89 0.0072 A 5 LNU307 0.83 0.0206 I 24 LNU299 0.88 0.0042 B 5LNU307 0.84 0.0044 K 24 LNU299 0.77 0.0089 C 5 LNU307 0.78 0.0388 L 24LNU299 0.82 0.0125 E 5 LNU311 0.80 0.0321 G 24 LNU299 0.89 0.0032 F 5LNU311 0.76 0.0280 H 24 LNU300 0.91 0.0043 A 5 LNU332 0.72 0.0419 H 24LNU300 0.88 0.0044 B 5 LNU332 0.84 0.0172 I 24 LNU300 0.75 0.0126 C 5LNU348 0.89 0.0071 I 24 LNU300 0.71 0.0463 E 5 LNU359 0.82 0.0231 G 24LNU301 0.88 0.0083 A 5 LNU360 0.87 0.0101 L 24 LNU301 0.78 0.0228 B 5LNU361 0.79 0.0360 G 24 LNU307 0.77 0.0425 A 5 LNU361 0.76 0.0300 H 24LNU307 0.82 0.0121 B 5 LNU371 0.74 0.0341 H 24 LNU307 0.73 0.0171 C 5LNU394 0.84 0.0172 G 24 LNU307 0.77 0.0144 N 5 LNU460 0.76 0.0472 G 24LNU307 0.83 0.0105 E 5 LNU476 0.87 0.0103 G 24 LNU307 0.82 0.0119 F 5LNU476 0.72 0.0200 M 24 LNU332 0.84 0.0083 B 5 LNU519 0.74 0.0370 H 24LNU339 0.76 0.0487 A 5 LNU299 0.91 0.0042 A 24 LNU339 0.76 0.0293 E 5LNU299 0.85 0.0080 B 24 LNU348 0.85 0.0072 F 5 LNU299 0.89 0.0007 C 24LNU359 0.91 0.0049 A 5 LNU299 0.72 0.0273 N 24 LNU359 0.73 0.0379 B 5LNU299 0.76 0.0271 E 24 LNU360 0.77 0.0440 A 5 LNU299 0.91 0.0016 F 24LNU360 0.82 0.0128 E 5 LNU300 0.82 0.0248 A 24 LNU361 0.79 0.0359 A 5LNU300 0.85 0.0080 B 24 LNU361 0.72 0.0419 E 5 LNU300 0.71 0.0487 F 24LNU371 0.78 0.0382 A 5 LNU301 0.79 0.0354 A 24 LNU371 0.75 0.0117 C 5LNU301 0.81 0.0137 B 24 LNU371 0.76 0.0293 E 5 LNU307 0.77 0.0408 A 24LNU394 0.77 0.0242 B 5 LNU307 0.73 0.0395 B 24 LNU459 0.87 0.0046 F 5LNU307 0.77 0.0088 C 24 LNU460 0.91 0.0044 A 5 LNU307 0.76 0.0168 N 24LNU460 0.72 0.0424 B 5 LNU307 0.86 0.0060 E 24 LNU469 0.81 0.0285 A 5LNU307 0.74 0.0343 F 24 LNU469 0.82 0.0118 E 5 LNU339 0.85 0.0164 A 24LNU519 0.85 0.0081 B 5 LNU339 0.74 0.0365 E 24 LNU299 0.90 0.0025 H 6LNU348 0.88 0.0044 F 24 LNU299 0.84 0.0047 K 6 LNU360 0.83 0.0113 E 24LNU301 0.76 0.0486 G 6 LNU361 0.79 0.0344 A 24 LNU301 0.79 0.0202 H 6LNU361 0.76 0.0277 E 24 LNU307 0.85 0.0164 G 6 LNU371 0.83 0.0217 A 24LNU311 0.91 0.0016 H 6 LNU371 0.72 0.0294 N 24 LNU332 0.92 0.0014 H 6LNU376 0.71 0.0463 B 24 LNU348 0.77 0.0419 I 6 LNU376 0.73 0.0408 F 24LNU359 0.89 0.0066 G 6 LNU394 0.74 0.0367 E 24 LNU361 0.86 0.0065 J 6LNU394 0.85 0.0081 B 24 LNU361 0.79 0.0121 K 6 LNU459 0.84 0.0088 F 24LNU394 0.76 0.0460 I 6 LNU460 0.76 0.0486 A 24 LNU418 0.81 0.0139 H 6LNU469 0.81 0.0281 A 24 LNU459 0.76 0.0276 J 6 LNU469 0.80 0.0170 E 24LNU460 0.88 0.0093 G 6 LNU519 0.81 0.0141 B 24 LNU460 0.88 0.0038 H 6LNU299 0.76 0.0275 B 26 LNU471 0.79 0.0197 H 6 LNU299 0.79 0.0109 N 26LNU476 0.85 0.0152 G 6 LNU299 0.89 0.0034 E 26 LNU476 0.82 0.0126 H 6LNU299 0.81 0.0156 F 26 LNU518 0.70 0.0351 K 6 LNU300 0.77 0.0444 D 26LNU519 0.90 0.0020 H 6 LNU300 0.71 0.0488 E 26 LNU519 0.81 0.0267 I 6LNU300 0.81 0.0159 F 26 LNU299 0.72 0.0458 H 8 LNU371 0.71 0.0207 C 26LNU300 0.89 0.0067 G 8 LNU371 0.74 0.0357 F 26 LNU300 0.76 0.0495 L 8LNU394 0.71 0.0463 E 26 LNU307 0.90 0.0061 G 8 LNU418 0.76 0.0286 B 26LNU307 0.79 0.0338 I 8 LNU460 0.90 0.0052 D 26 LNU307 0.86 0.0032 K 8LNU469 0.76 0.0293 E 26 LNU307 0.78 0.0369 L 8 LNU471 0.89 0.0068 A 26LNU307 0.77 0.0099 M 8 LNU299 0.81 0.0154 H 26 LNU311 0.79 0.0362 G 8LNU299 0.86 0.0030 K 26 LNU311 0.79 0.0184 H 8 LNU300 0.83 0.0215 L 26LNU317 0.73 0.0401 E 7 LNU311 0.92 0.0037 G 26 LNU332 0.83 0.0113 B 7LNU317 0.71 0.0324 K 26 LNU332 0.82 0.0134 H 8 LNU361 0.71 0.0304 K 26LNU332 0.81 0.0281 I 8 LNU371 0.78 0.0387 G 26 LNU339 0.78 0.0224 F 7LNU371 0.78 0.0400 L 26 LNU339 0.74 0.0363 H 8 LNU394 0.89 0.0070 G 26LNU348 0.89 0.0032 E 7 LNU418 0.77 0.0431 G 26 LNU348 0.75 0.0318 H 8LNU460 0.71 0.0479 H 26 LNU348 0.91 0.0050 I 8 LNU469 0.86 0.0128 L 26LNU358 0.77 0.0252 B 7 LNU471 0.80 0.0306 I 26 LNU359 0.83 0.0196 A 7LNU476 0.90 0.0056 I 26 LNU359 0.84 0.0169 G 8 LNU299 0.71 0.0492 J 28LNU359 0.76 0.0278 H 8 LNU299 0.87 0.0108 L 28 LNU360 0.80 0.0315 A 7LNU299 0.89 0.0067 A 27 LNU360 0.89 0.0077 D 7 LNU299 0.86 0.0064 B 27LNU360 0.79 0.0210 H 8 LNU299 0.75 0.0122 C 27 LNU360 0.88 0.0095 L 8LNU299 0.73 0.0241 N 27 LNU361 0.72 0.0419 F 7 LNU299 0.88 0.0037 E 27LNU361 0.80 0.0319 G 8 LNU299 0.80 0.0172 F 27 LNU361 0.81 0.0146 H 8LNU300 0.91 0.0045 A 27 LNU371 0.77 0.0429 A 7 LNU300 0.86 0.0057 B 27LNU394 0.83 0.0204 G 8 LNU300 0.82 0.0035 C 27 LNU361 0.88 0.0084 A 7LNU300 0.90 0.0008 N 27 LNU358 0.83 0.0213 A 7 LNU300 0.91 0.0018 E 27LNU418 0.77 0.0255 H 8 LNU300 0.90 0.0024 F 27 LNU460 0.89 0.0075 A 7LNU301 0.74 0.0355 B 27 LNU460 0.80 0.0299 G 8 LNU307 0.78 0.0214 H 28LNU460 0.77 0.0262 H 8 LNU307 0.74 0.0374 B 27 LNU469 0.91 0.0048 A 7LNU307 0.76 0.0115 C 27 LNU471 0.81 0.0159 H 8 LNU307 0.85 0.0039 N 27LNU476 0.90 0.0061 G 8 LNU307 0.87 0.0048 F 27 LNU476 0.79 0.0189 H 8LNU311 0.86 0.0015 M 28 LNU476 0.71 0.0220 M 8 LNU311 0.71 0.0489 B 27LNU519 0.80 0.0176 E 7 LNU317 0.76 0.0469 I 28 LNU519 0.88 0.0039 H 8LNU332 0.73 0.0383 B 27 LNU519 0.77 0.0418 I 8 LNU339 0.74 0.0373 E 27LNU332 0.83 0.0214 B 9 LNU348 0.85 0.0068 F 27 LNU348 0.77 0.0427 E 9LNU359 0.79 0.0340 G 28 LNU358 0.87 0.0116 B 9 LNU359 0.80 0.0304 A 27LNU359 0.83 0.0212 E 9 LNU359 0.83 0.0107 B 27 LNU361 0.82 0.0253 F 9LNU394 0.71 0.0211 C 27 LNU376 0.89 0.0013 C 9 LNU361 0.78 0.0220 B 27LNU471 0.82 0.0235 D 9 LNU376 0.90 0.0021 B 27 LNU471 0.83 0.0200 E 9LNU376 0.84 0.0086 F 27 LNU519 0.76 0.0483 E 9 LNU394 0.73 0.0418 E 27LNU299 0.89 0.0067 G 12 LNU394 0.82 0.0119 J 28 LNU299 0.72 0.0273 K 12LNU394 0.84 0.0191 L 28 LNU299 0.77 0.0417 A 11 LNU394 0.74 0.0348 B 27LNU299 0.70 0.0342 N 11 LNU418 0.80 0.0300 A 27 LNU299 0.77 0.0244 E 11LNU459 0.78 0.0234 E 27 LNU300 0.90 0.0057 G 12 LNU459 0.74 0.0372 F 27LNU300 0.82 0.0130 H 12 LNU460 0.83 0.0205 A 27 LNU300 0.72 0.0197 M 12LNU460 0.87 0.0046 B 27 LNU301 0.85 0.0149 G 12 LNU469 0.92 0.0013 H 28LNU301 0.75 0.0499 A 11 LNU469 0.77 0.0449 A 27 LNU301 0.74 0.0215 N 11LNU469 0.85 0.0164 D 27 LNU307 0.77 0.0410 G 12 LNU469 0.79 0.0207 E 27LNU307 0.86 0.0141 L 12 LNU471 0.83 0.0108 B 27 LNU307 0.88 0.0041 E 11LNU299 0.91 0.0042 A 32 LNU311 0.76 0.0451 G 12 LNU299 0.85 0.0080 B 32LNU311 0.78 0.0238 B 11 LNU299 0.89 0.0007 C 32 LNU317 0.71 0.0475 J 12LNU299 0.72 0.0273 N 32 LNU317 0.81 0.0083 K 12 LNU299 0.76 0.0271 E 32LNU317 0.80 0.0057 C 11 LNU299 0.91 0.0016 F 32 LNU317 0.72 0.0426 F 11LNU300 0.82 0.0248 A 32 LNU332 0.75 0.0306 B 11 LNU300 0.85 0.0080 B 32LNU339 0.79 0.0188 E 11 LNU300 0.71 0.0487 F 32 LNU359 0.90 0.0052 G 12LNU301 0.79 0.0354 A 32 LNU359 0.82 0.0234 D 11 LNU301 0.81 0.0137 B 32LNU360 0.77 0.0407 G 12 LNU307 0.77 0.0408 A 32 LNU360 0.71 0.0312 N 11LNU307 0.73 0.0395 B 32 LNU360 0.71 0.0479 E 11 LNU307 0.77 0.0088 C 32LNU360 0.72 0.0443 F 11 LNU307 0.76 0.0168 N 32 LNU361 0.90 0.0053 G 12LNU307 0.86 0.0060 E 32 LNU371 0.86 0.0135 G 12 LNU307 0.74 0.0343 F 32LNU371 0.78 0.0402 I 12 LNU339 0.85 0.0164 A 32 LNU371 0.70 0.0343 K 12LNU339 0.74 0.0365 E 32 LNU371 0.77 0.0439 L 12 LNU348 0.88 0.0044 F 32LNU376 0.80 0.0183 B 11 LNU360 0.83 0.0113 E 32 LNU394 0.76 0.0467 G 12LNU361 0.79 0.0344 A 32 LNU394 0.78 0.0372 I 12 LNU361 0.76 0.0277 E 32LNU394 0.78 0.0139 K 12 LNU371 0.83 0.0217 A 32 LNU418 0.76 0.0473 G 12LNU371 0.72 0.0294 N 32 LNU418 0.79 0.0121 N 11 LNU376 0.71 0.0463 B 32LNU459 0.87 0.0010 M 12 LNU376 0.73 0.0408 F 32 LNU460 0.87 0.0050 J 12LNU394 0.74 0.0367 E 32 LNU476 0.84 0.0187 G 12 LNU394 0.85 0.0081 B 32LNU476 0.79 0.0363 I 12 LNU459 0.84 0.0088 F 32 LNU476 0.76 0.0294 J 12LNU460 0.76 0.0486 A 32 LNU518 0.73 0.0254 N 11 LNU469 0.81 0.0281 A 32LNU519 0.76 0.0469 I 12 LNU469 0.80 0.0170 E 32 LNU299 0.90 0.0055 A 13LNU519 0.81 0.0141 B 32 LNU300 0.89 0.0068 A 13 LNU300 0.91 0.0046 G 32LNU300 0.77 0.0149 N 13 LNU300 0.76 0.0451 L 32 LNU300 0.76 0.0283 E 13LNU307 0.89 0.0072 G 32 LNU300 0.87 0.0048 F 13 LNU307 0.76 0.0476 I 32LNU301 0.86 0.0134 A 13 LNU307 0.89 0.0012 K 32 LNU307 0.79 0.0061 C 13LNU307 0.79 0.0351 L 32 LNU307 0.72 0.0297 N 13 LNU307 0.77 0.0095 M 32LNU307 0.72 0.0433 E 13 LNU311 0.79 0.0348 G 32 LNU307 0.77 0.0269 F 13LNU332 0.80 0.0324 I 32 LNU311 0.83 0.0104 B 13 LNU348 0.88 0.0081 I 32LNU317 0.76 0.0295 B 13 LNU359 0.86 0.0123 G 32 LNU317 0.82 0.0131 F 13LNU360 0.86 0.0138 L 32 LNU332 0.86 0.0064 B 13 LNU361 0.82 0.0245 G 32LNU332 0.84 0.0084 E 13 LNU361 0.72 0.0460 H 32 LNU339 0.88 0.0085 A 13LNU394 0.85 0.0153 G 32 LNU339 0.76 0.0463 D 13 LNU394 0.78 0.0373 I 32LNU339 0.91 0.0017 E 13 LNU394 0.72 0.0288 K 32 LNU348 0.76 0.0472 D 13LNU460 0.82 0.0249 G 32 LNU348 0.84 0.0099 F 13 LNU471 0.71 0.0500 H 32LNU359 0.73 0.0385 B 13 LNU476 0.90 0.0063 G 32 LNU360 0.81 0.0147 B 13LNU476 0.74 0.0138 M 32 LNU360 0.80 0.0181 F 13 LNU519 0.78 0.0223 H 32LNU361 0.86 0.0138 A 13 LNU519 0.78 0.0391 I 32 LNU376 0.77 0.0256 B 13LNU299 0.78 0.0126 K 34 LNU394 0.85 0.0145 A 13 LNU299 0.77 0.0451 A 34LNU459 0.71 0.0316 N 13 LNU299 0.79 0.0202 B 34 LNU460 0.91 0.0046 A 13LNU299 0.73 0.0168 C 34 LNU469 0.91 0.0039 A 13 LNU299 0.85 0.0143 D 34LNU469 0.82 0.0035 C 13 LNU299 0.77 0.0161 N 34 LNU469 0.73 0.0419 E 13LNU299 0.80 0.0161 E 34 LNU299 0.75 0.0311 H 14 LNU299 0.75 0.0339 F 34LNU300 0.82 0.0232 G 14 LNU300 0.80 0.0291 I 34 LNU301 0.71 0.0499 H 14LNU300 0.79 0.0109 K 34 LNU307 0.90 0.0059 G 14 LNU300 0.81 0.0137 B 34LNU307 0.77 0.0424 I 14 LNU301 0.82 0.0234 G 34 LNU307 0.70 0.0353 K 14LNU301 0.71 0.0311 K 34 LNU307 0.83 0.0217 L 14 LNU301 0.80 0.0163 B 34LNU307 0.84 0.0024 M 14 LNU301 0.71 0.0228 C 34 LNU332 0.86 0.0124 I 14LNU301 0.77 0.0245 E 34 LNU348 0.89 0.0074 I 14 LNU307 0.80 0.0303 G 34LNU359 0.77 0.0429 G 14 LNU307 0.83 0.0109 H 34 LNU360 0.82 0.0235 I 14LNU307 0.79 0.0359 L 34 LNU360 0.91 0.0041 L 14 LNU317 0.76 0.0180 K 34LNU371 0.83 0.0102 H 14 LNU317 0.71 0.0209 M 34 LNU418 0.84 0.0097 H 14LNU317 0.77 0.0090 C 34 LNU459 0.71 0.0217 M 14 LNU360 0.77 0.0450 A 34LNU460 0.71 0.0470 H 14 LNU361 0.83 0.0209 G 34 LNU471 0.77 0.0448 G 14LNU361 0.86 0.0058 E 34 LNU476 0.80 0.0297 G 14 LNU371 0.86 0.0141 G 34LNU518 0.70 0.0342 K 14 LNU371 0.83 0.0214 I 34 LNU519 0.79 0.0356 I 14LNU371 0.81 0.0087 K 34 LNU299 0.89 0.0067 A 15 LNU371 0.77 0.0433 L 34LNU299 0.86 0.0064 B 15 LNU376 0.78 0.0212 B 34 LNU299 0.75 0.0122 C 15LNU394 0.82 0.0253 I 34 LNU299 0.73 0.0241 N 15 LNU394 0.76 0.0166 K 34LNU299 0.88 0.0037 E 15 LNU459 0.71 0.0216 M 34 LNU299 0.80 0.0172 F 15LNU460 0.83 0.0219 G 34 LNU299 0.71 0.0492 J 16 LNU460 0.75 0.0313 B 34LNU299 0.87 0.0108 L 16 LNU469 0.87 0.0048 H 34 LNU300 0.91 0.0045 A 15LNU471 0.88 0.0083 A 34 LNU300 0.86 0.0057 B 15 LNU476 0.80 0.0291 G 34LNU300 0.82 0.0035 C 15 LNU476 0.84 0.0189 A 34 LNU300 0.90 0.0008 N 15LNU476 0.86 0.0067 B 34 LNU300 0.91 0.0018 E 15 LNU518 0.76 0.0286 B 34LNU300 0.90 0.0024 F 15 LNU519 0.74 0.0235 N 34 LNU301 0.74 0.0355 B 15LNU299 0.86 0.0067 J 36 LNU307 0.74 0.0374 B 15 LNU299 0.91 0.0041 A 36LNU307 0.76 0.0115 C 15 LNU299 0.79 0.0185 B 36 LNU307 0.85 0.0039 N 15LNU299 0.89 0.0006 C 36 LNU307 0.87 0.0048 F 15 LNU299 0.78 0.0387 D 36LNU307 0.78 0.0214 H 16 LNU299 0.80 0.0101 N 36 LNU311 0.71 0.0489 B 15LNU299 0.90 0.0023 E 36 LNU311 0.86 0.0015 M 16 LNU299 0.83 0.0110 F 36LNU317 0.76 0.0469 I 16 LNU300 0.92 0.0037 G 36 LNU332 0.73 0.0383 B 15LNU300 0.80 0.0315 I 36 LNU339 0.74 0.0373 E 15 LNU300 0.74 0.0239 K 36LNU348 0.85 0.0068 F 15 LNU300 0.83 0.0202 A 36 LNU359 0.80 0.0304 A 15LNU300 0.89 0.0030 B 36 LNU359 0.83 0.0107 B 15 LNU300 0.86 0.0014 C 36LNU359 0.79 0.0340 G 16 LNU301 0.80 0.0308 G 36 LNU361 0.78 0.0220 B 15LNU301 0.77 0.0426 I 36 LNU376 0.90 0.0021 B 15 LNU301 0.77 0.0152 K 36LNU376 0.84 0.0086 F 15 LNU301 0.86 0.0068 B 36 LNU394 0.71 0.0211 C 15LNU301 0.89 0.0007 C 36 LNU394 0.74 0.0348 B 15 LNU301 0.86 0.0135 D 36LNU394 0.82 0.0119 J 16 LNU307 0.89 0.0071 G 36 LNU394 0.84 0.0191 L 16LNU307 0.76 0.0282 H 36 LNU394 0.73 0.0418 E 15 LNU307 0.83 0.0197 I 36LNU418 0.80 0.0300 A 15 LNU307 0.77 0.0143 K 36 LNU459 0.78 0.0234 E 15LNU307 0.80 0.0293 L 36 LNU459 0.74 0.0372 F 15 LNU307 0.80 0.0053 M 36LNU460 0.83 0.0205 A 15 LNU307 0.84 0.0170 A 36 LNU460 0.87 0.0046 B 15LNU307 0.85 0.0082 E 36 LNU469 0.77 0.0449 A 15 LNU307 0.84 0.0085 F 36LNU469 0.85 0.0164 D 15 LNU311 0.73 0.0396 B 36 LNU469 0.79 0.0207 E 15LNU332 0.78 0.0237 B 36 LNU469 0.92 0.0013 H 16 LNU339 0.83 0.0220 D 36LNU471 0.83 0.0108 B 15 LNU348 0.78 0.0383 I 36 LNU299 0.88 0.0088 A 17LNU348 0.79 0.0197 B 36 LNU299 0.77 0.0098 C 17 LNU359 0.88 0.0090 G 36LNU299 0.76 0.0295 F 17 LNU359 0.77 0.0448 A 36 LNU299 0.76 0.0494 G 18LNU359 0.72 0.0446 B 36 LNU299 0.71 0.0205 M 18 LNU360 0.82 0.0225 A 36LNU300 0.79 0.0209 F 17 LNU360 0.77 0.0268 E 36 LNU300 0.83 0.0197 G 18LNU361 0.70 0.0236 C 36 LNU300 0.83 0.0111 H 18 LNU361 0.80 0.0173 E 36LNU301 0.86 0.0127 A 17 LNU371 0.77 0.0438 A 36 LNU307 0.71 0.0468 B 17LNU371 0.86 0.0060 E 36 LNU307 0.79 0.0063 C 17 LNU371 0.72 0.0450 F 36LNU307 0.85 0.0079 E 17 LNU376 0.88 0.0037 B 36 LNU307 0.76 0.0292 F 17LNU394 0.72 0.0302 K 36 LNU307 0.85 0.0144 G 18 LNU459 0.77 0.0437 I 36LNU307 0.81 0.0269 I 18 LNU459 0.82 0.0124 F 36 LNU307 0.75 0.0198 K 18LNU460 0.89 0.0068 G 36 LNU307 0.76 0.0455 L 18 LNU460 0.79 0.0364 A 36LNU307 0.77 0.0091 M 18 LNU460 0.78 0.0238 B 36 LNU311 0.82 0.0119 B 17LNU460 0.80 0.0292 D 36 LNU317 0.76 0.0481 D 17 LNU469 0.71 0.0473 E 36LNU317 0.77 0.0252 F 17 LNU471 0.84 0.0178 G 36 LNU317 0.78 0.0138 K 18LNU471 0.85 0.0156 A 36 LNU332 0.83 0.0111 B 17 LNU476 0.80 0.0322 G 36LNU332 0.85 0.0142 D 17 LNU518 0.73 0.0411 B 36 LNU332 0.74 0.0356 E 17LNU519 0.91 0.0043 I 36 LNU332 0.83 0.0211 I 18 LNU519 0.81 0.0147 B 36LNU339 0.86 0.0134 A 17 LNU519 0.85 0.0035 N 36 LNU339 0.92 0.0014 E 17LNU299 0.87 0.0026 K 37 LNU348 0.91 0.0047 D 17 LNU299 0.86 0.0058 B 36LNU348 0.78 0.0222 F 17 LNU299 0.81 0.0155 E 36 LNU348 0.84 0.0177 I 18LNU299 0.72 0.0446 F 36 LNU359 0.77 0.0415 G 18 LNU300 0.88 0.0088 G 37LNU360 0.88 0.0036 E 17 LNU300 0.79 0.0344 L 37 LNU360 0.80 0.0177 F 17LNU300 0.77 0.0088 M 37 LNU360 0.88 0.0097 I 18 LNU300 0.90 0.0055 A 36LNU360 0.76 0.0462 L 18 LNU300 0.85 0.0070 B 36 LNU361 0.84 0.0175 A 17LNU300 0.89 0.0005 C 36 LNU361 0.82 0.0129 E 17 LNU301 0.77 0.0433 A 36LNU371 0.79 0.0203 H 18 LNU301 0.74 0.0154 C 36 LNU376 0.77 0.0266 B 17LNU301 0.72 0.0276 N 36 LNU460 0.81 0.0282 A 17 LNU307 0.83 0.0196 G 37LNU460 0.76 0.0487 G 18 LNU307 0.73 0.0172 M 37 LNU469 0.79 0.0327 A 17LNU307 0.83 0.0117 B 36 LNU469 0.71 0.0203 C 17 LNU307 0.72 0.0461 F 36LNU469 0.71 0.0499 J 18 LNU311 0.83 0.0209 G 37 LNU476 0.81 0.0275 G 18LNU311 0.89 0.0028 B 36 LNU519 0.80 0.0325 D 17 LNU332 0.89 0.0031 B 36LNU519 0.80 0.0317 I 18 LNU339 0.75 0.0308 B 36 LNU307 0.73 0.0410 H 10LNU348 0.87 0.0109 I 37 LNU332 0.73 0.0404 K 10 LNU394 0.84 0.0023 C 36LNU332 0.84 0.0049 M 10 LNU359 0.84 0.0183 G 37 LNU471 0.79 0.0209 K 10LNU360 0.81 0.0281 L 37 LNU300 0.91 0.0046 G 20 LNU360 0.74 0.0354 E 36LNU300 0.76 0.0451 L 20 LNU361 0.85 0.0153 G 37 LNU307 0.89 0.0072 G 20LNU371 0.85 0.0071 E 36 LNU307 0.76 0.0476 I 20 LNU376 0.73 0.0398 B 36LNU307 0.89 0.0012 K 20 LNU394 0.80 0.0302 D 36 LNU307 0.79 0.0351 L 20LNU394 0.85 0.0158 G 37 LNU307 0.77 0.0095 M 20 LNU394 0.81 0.0264 I 37LNU311 0.79 0.0348 G 20 LNU418 0.73 0.0379 E 36 LNU332 0.80 0.0324 I 20LNU459 0.74 0.0368 F 36 LNU348 0.88 0.0081 I 20 LNU469 0.72 0.0439 E 36LNU359 0.86 0.0123 G 20 LNU476 0.82 0.0232 G 37 LNU360 0.86 0.0138 L 20LNU519 0.78 0.0221 B 36 LNU361 0.82 0.0245 G 20 LNU299 0.79 0.0328 F 39LNU361 0.72 0.0460 H 20 LNU307 0.77 0.0445 F 39 LNU394 0.85 0.0153 G 20LNU311 0.73 0.0398 H 40 LNU394 0.78 0.0373 I 20 LNU339 0.79 0.0195 H 40LNU394 0.72 0.0288 K 20 LNU348 0.90 0.0022 K 40 LNU460 0.82 0.0249 G 20LNU361 0.74 0.0369 H 40 LNU471 0.71 0.0500 H 20 LNU376 0.78 0.0370 F 39LNU476 0.90 0.0063 G 20 LNU394 0.83 0.0196 F 39 LNU476 0.74 0.0138 M 20LNU394 0.90 0.0059 B 39 LNU519 0.78 0.0223 H 20 LNU460 0.74 0.0360 N 39LNU519 0.78 0.0391 I 20 LNU469 0.91 0.0048 B 39 LNU299 0.92 0.0037 A 21LNU469 0.89 0.0071 F 39 LNU299 0.83 0.0116 B 21 LNU518 0.88 0.0099 E 39LNU299 0.86 0.0014 C 21 LNU518 0.81 0.0278 F 39 LNU299 0.91 0.0018 F 21LNU519 0.72 0.0431 H 40 LNU300 0.89 0.0071 G 22 LNU299 0.88 0.0082 A 40LNU300 0.76 0.0452 L 22 LNU299 0.78 0.0219 B 40 LNU300 0.80 0.0301 A 21LNU299 0.91 0.0002 C 40 LNU300 0.80 0.0171 B 21 LNU299 0.88 0.0043 F 40LNU300 0.72 0.0446 F 21 LNU300 0.82 0.0135 B 40 LNU301 0.83 0.0198 A 21LNU300 0.77 0.0259 F 40 LNU301 0.77 0.0240 B 21 LNU300 0.76 0.0483 L 41LNU307 0.90 0.0052 G 22 LNU301 0.85 0.0081 B 40 LNU307 0.80 0.0326 I 22LNU307 0.76 0.0451 A 40 LNU307 0.89 0.0012 K 22 LNU307 0.80 0.0059 C 40LNU307 0.80 0.0314 L 22 LNU307 0.76 0.0164 N 40 LNU307 0.77 0.0097 M 22LNU307 0.85 0.0072 E 40 LNU307 0.72 0.0429 B 21 LNU307 0.72 0.0446 F 40LNU307 0.82 0.0034 C 21 LNU307 0.92 0.0035 G 41 LNU307 0.80 0.0096 N 21LNU307 0.78 0.0404 I 41 LNU307 0.84 0.0087 E 21 LNU307 0.92 0.0005 K 41LNU307 0.77 0.0262 F 21 LNU307 0.82 0.0236 L 41 LNU311 0.78 0.0373 G 22LNU307 0.78 0.0074 M 41 LNU311 0.75 0.0308 H 22 LNU311 0.77 0.0426 G 41LNU332 0.73 0.0405 H 22 LNU332 0.81 0.0261 I 41 LNU332 0.80 0.0293 I 22LNU339 0.87 0.0100 A 40 LNU339 0.89 0.0077 A 21 LNU339 0.75 0.0326 E 40LNU339 0.73 0.0247 N 21 LNU348 0.92 0.0013 F 40 LNU339 0.79 0.0208 E 21LNU348 0.92 0.0036 I 41 LNU348 0.91 0.0043 I 22 LNU358 0.71 0.0339 N 40LNU348 0.90 0.0022 F 21 LNU359 0.88 0.0091 G 41 LNU359 0.85 0.0157 G 22LNU360 0.76 0.0272 E 40 LNU360 0.89 0.0072 L 22 LNU360 0.86 0.0137 L 41LNU360 0.84 0.0097 E 21 LNU361 0.85 0.0154 A 40 LNU361 0.80 0.0317 G 22LNU361 0.80 0.0313 G 41 LNU361 0.75 0.0328 H 22 LNU371 0.83 0.0214 A 40LNU361 0.79 0.0362 A 21 LNU371 0.71 0.0481 B 40 LNU361 0.75 0.0326 E 21LNU376 0.71 0.0480 B 40 LNU371 0.80 0.0322 A 21 LNU376 0.77 0.0266 F 40LNU376 0.74 0.0359 F 21 LNU394 0.77 0.0447 A 40 LNU394 0.74 0.0366 E 21LNU394 0.81 0.0141 B 40 LNU394 0.82 0.0232 G 22 LNU394 0.82 0.0255 G 41LNU394 0.76 0.0487 A 21 LNU394 0.71 0.0311 K 41 LNU394 0.82 0.0119 B 21LNU459 0.88 0.0036 F 40 LNU418 0.72 0.0459 H 22 LNU460 0.84 0.0177 G 41LNU459 0.85 0.0082 F 21 LNU469 0.78 0.0399 A 40 LNU460 0.81 0.0286 G 22LNU471 0.73 0.0392 H 41 LNU460 0.77 0.0410 A 21 LNU476 0.71 0.0465 F 40Table 66. “Corr. Set ID” - correlation set ID according to thecorrelated parameters Table above.

TABLE 67 Correlation between the expression level of selected LNUhomologous genes of some embodiments of the invention in various tissuesand the phenotypic performance under normal conditions across maizeaccessions Exp. Exp. Gene set Corr. Gene set Corr. Name R P ID ID Name RP ID ID LNU494_H2 0.76 0.0460 A 21 LNU309_H3 0.71 0.0336 K 6 LNU494_H20.79 0.0358 A 23 LNU431_H1 0.76 0.0289 H 6 LNU417_H4 0.73 0.0414 H 24LNU417_H4 0.76 0.0492 A 7 LNU417_H4 0.78 0.0237 B 26 LNU417_H4 0.900.0024 F 7 LNU309_H3 0.79 0.0111 K 28 LNU417_H4 0.79 0.0198 H 8LNU494_H2 0.85 0.0078 B 27 LNU431_H1 0.77 0.0447 A 7 LNU309_H3 0.710.0331 K 32 LNU417_H4 0.91 0.0048 F 9 LNU309_H3 0.78 0.0129 K 34LNU494_H2 0.90 0.0056 E 9 LNU417_H4 0.75 0.0128 C 34 LNU309_H3 0.800.0299 A 11 LNU417_H4 0.87 0.0111 G 36 LNU309_H3 0.73 0.0390 F 11LNU431_H1 0.78 0.0388 G 36 LNU431_H1 0.79 0.0348 G 12 LNU309_H3 0.800.0172 N 39 LNU494_H2 0.71 0.0305 N 11 LNU417_H4 0.71 0.0497 H 40LNU309_H3 0.78 0.0227 F 13 LNU309_H3 0.71 0.0331 K 41 LNU494_H2 0.770.0264 B 13 LNU431_H1 0.76 0.0476 A 40 LNU309_H3 0.73 0.0169 M 14LNU309_H3 0.87 0.0010 C 1 LNU431_H1 0.79 0.0327 G 14 LNU494_H2 0.800.0321 A 1 LNU309_H3 0.79 0.0111 K 16 LNU309_H3 0.74 0.0148 C 3LNU494_H2 0.85 0.0078 B 15 LNU309_H3 0.71 0.0321 N 3 LNU309_H3 0.730.0417 F 17 LNU494_H2 0.85 0.0162 A 3 LNU309_H3 0.71 0.0331 K 20LNU309_H3 0.77 0.0253 H 6 LNU417_H4 0.72 0.0428 H 22 Table 67. “Corr.Set ID”—correlation set ID according to the correlated parameters Tableabove.

Example 13 Gene Cloning and Generation of Binary Vectors for PlantExpression

To validate their role in improving yield, selected genes wereover-expressed in plants, as follows.

Cloning Strategy

Selected genes from those presented in Examples 1-12 hereinabove werecloned into binary vectors for the generation of transgenic plants. Forcloning, the full-length open reading frame (ORF) was first identified.In case of ORF-EST clusters and in some cases already published mRNAsequences were analyzed to identify the entire open reading frame bycomparing the results of several translation algorithms to knownproteins from other plant species. To clone the full-length cDNAs,reverse transcription (RT) followed by polymerase chain reaction (PCR;RT-PCR) was performed on total RNA extracted from leaves, flowers,siliques or other plant tissues, growing under normal and differenttreated conditions. Total RNA was extracted as described in “GENERALEXPERIMENTAL AND BIOINFORMATICS METHODS” above. Production of cDNA andPCR amplification was performed using standard protocols describedelsewhere (Sambrook J., E. F. Fritsch, and T. Maniatis. 1989. MolecularCloning. A Laboratory Manual., 2nd Ed. Cold Spring Harbor LaboratoryPress, New York.) which are well known to those skilled in the art. PCRproducts are purified using PCR purification kit (Qiagen). In case wherethe entire coding sequence was not found, RACE kit from Invitrogen(RACE=Rapid Amplification of cDNA Ends) was used to access the full cDNAtranscript of the gene from the RNA samples described above. RACEproducts were cloned into high copy vector followed by sequencing ordirectly sequenced.

The information from the RACE procedure was used for cloning of the fulllength ORF of the corresponding genes.

In case genomic DNA was cloned, the genes were amplified by direct PCRon genomic DNA extracted from leaf tissue using the DNAeasy kit (QiagenCat. No. 69104).

Usually, 2 sets of primers were synthesized for the amplification ofeach gene from a cDNA or a genomic sequence; an external set of primersand an internal set (nested PCR primers). When needed (e.g., when thefirst PCR reaction does not result in a satisfactory product forsequencing), an additional primer (or two) of the nested PCR primerswere used.

To facilitate cloning of the cDNAs/genomic sequences, a 8-12 bpextension was added to the 5′ of each primer. The primer extensionincludes an endonuclease restriction site. The restriction sites wereselected using two parameters: (a). The site does not exist in the cDNAsequence; and (b). The restriction sites in the forward and reverseprimers were designed such that the digested cDNA was inserted in thesense formation into the binary vector utilized for transformation.

Each digested PCR product was inserted into a high copy vector pUC19(New England BioLabs Inc], or into plasmids originating from thisvector. In some cases the undigested PCR product was inserted intopCR-Blunt II-TOPO (Invitrogen).

Sequencing of the amplified PCR products was performed, using ABI 377sequencer (Amersham Biosciences Inc). In some cases, after confirmingthe sequences of the cloned genes, the cloned cDNA was introduced into amodified pGI binary vector containing the At6669 promoter via digestionwith appropriate restriction endonucleases. In any case the insert wasfollowed by single copy of the NOS terminator (SEQ ID NO:3825). Thedigested products and the linearized plasmid vector are ligated using T4DNA ligase enzyme (Roche, Switzerland).

High copy plasmids containing the cloned genes were digested with therestriction endonucleases (New England BioLabs Inc) according to thesites designed in the primers and cloned into binary vectors as shown inTable 68, below.

Several DNA sequences of the selected genes were synthesized by acommercial supplier GeneArt [Hypertext Transfer Protocol://World WideWeb (dot) geneart (dot) coma Synthetic DNA was designed in silico.Suitable restriction enzymes sites were added to the cloned sequences atthe 5′ end and at the 3′ end to enable later cloning into the pQFNcbinary vector downstream of the At6669 promoter (SEQ ID NO: 3829).

Binary vectors used for cloning: The plasmid pPI is constructed byinserting a synthetic poly-(A) signal sequence, originating from pGL3basic plasmid vector (Promega, Acc No U47295; bp 4658-4811) into theHindlll restriction site of the binary vector pBI101.3 (Clontech, Acc.No. U12640). pGI (pBXYN) is similar to pPI, but the original gene in thebackbone, the GUS gene, is replaced by the GUS-Intron gene followed bythe NOS terminator (SEQ ID NO:3825) (Vancanneyt. G, et al MGG 220,245-50, 1990). pGI was used in the past to clone the polynucleotidesequences, initially under the control of 35S promoter [Odell, J T, etal. Nature 313, 810-812 (28 Feb. 1985); SEQ ID NO:3834].

The modified pGI vectors [pQXNc (FIG. 8); or pQFN (FIG. 2), pQFNc (FIG.2) or pQYN_6669 (FIG. 1)] are modified versions of the pGI vector inwhich the cassette is inverted between the left and right borders so thegene and its corresponding promoter are close to the right border andthe NPTII gene is close to the left border.

At6669, the Arabidopsis thaliana promoter sequence (SEQ ID NO:3829) wasinserted in the modified pGI binary vector, upstream to the clonedgenes, followed by DNA ligation and binary plasmid extraction frompositive E. coli colonies, as described above.

Colonies were analyzed by PCR using the primers covering the insertwhich were designed to span the introduced promoter and gene. Positiveplasmids were identified, isolated and sequenced.

Genes which were cloned by the present inventors are provided in Table68 below, along with the primers used for cloning.

TABLE 68 Genes cloned in High copy number plasmids Primers used Polyn.Polyp. High copy SEQ ID SEQ SEQ Gene Name plasmid Organism NOs: ID NO:ID NO: LNU290 Topo B WHEAT Triticum aestivum L. ND 3819, 3991 266 717LNU291 pUC19c SORGHUM Sorghum bicolor ND 3820, 3992, 4153, 4264 267 471LNU292 pUC19c SORGHUM Sorghum bicolor ND 3821, 3993, 4154, 4265 268 472LNU293 pUC19c RICE Oryza sativa L. Japonica Nipponbare 4155, 4266 269473 LNU294 pUC19c SOYBEAN Glycine max 40-219 3822, 3994, 4156, 3994 270718 LNU295 pUC19c TOMATO Lycopersicum esculentum MD 3823, 3995, 4157,4267 271 475 LNU296 272 476 LNU298 273 478 LNU299 pUC19c MAIZE Zea maysL. B73 3824, 3996, 4158, 4268 274 479 LNU300 pUC19c MAIZE Zea mays L.B73 3835, 3997, 4159, 4269 275 480 LNU301 pUC19c MAIZE Zea mays L. B733836, 3998, 3836, 4270 276 481 LNU302 pUC19c TOMATO Lycopersicumesculentum MD 3837, 3999, 4160, 4271 277 482 LNU303 Topo B SORGHUMSorghum bicolor ND 4161, 4272 278 483 LNU304 pUC19c RICE Oryza sativa L.Japonica Nipponbare 3838, 4000, 4162, 4273 279 484 LNU305 pUC19c BARLEYHordeum vulgare L. Manit 3839, 4001 280 719 LNU306 pUC19d ARABIDOPSISArabidopsis thaliana Kondara 3840, 4002 281 486 LNU307 Topo B MAIZE Zeamays L. B73 3841, 4003 282 720 LNU308 pUC19c ARABIDOPSIS Arabidopsisthaliana Kondara 3842, 4004, 4163, 4274 283 488 LNU309H3 462 676 LNU310pUC19c TOMATO Lycopersicum esculentum MD 3843, 4005, 4164, 4275 284 721LNU311 285 491 LNU312 pUC19c RICE Oryza sativa L. Japonica Nipponbare3844, 4006, 4165, 4006 286 492 LNU314 Topo B SORGHUM Sorghum bicolor ND3845, 4007, 4166, 4276 287 493 LNU315 Topo B WHEAT Triticum aestivum L.ND 3846, 4008 288 494 LNU316 pUC19c SORGHUM Sorghum bicolor ND 3847,4009, 3847, 4277 289 495 LNU317 pUC19c MAIZE Zea mays L. B73 3848, 4010,4167, 4278 290 496 LNU318 291 497 LNU319 pUC19c SORGHUM Sorghum bicolorND 3849, 4011 292 498 LNU322 pUC19c BARLEY Hordeum vulgare L. Manit3850, 4012, 4168, 4279 293 499 LNU323 pUC19c TOMATO Lycopersicumesculentum MD 3851, 4013, 3851, 4280 294 722 LNU324 Topo B SORGHUMSorghum bicolor ND 3852, 4014, 4169, 4281 295 723 LNU326 pUC19c TOMATOLycopersicum esculentum MD 3853, 4015, 4170, 4282 296 724 LNU327 Topo BWHEAT Triticum aestivum L. EYAL 3854, 4016, 3854, 4283 297 503 LNU328pUC19c TOMATO Lycopersicum esculentum MD 3855, 4017, 4171, 4284 298 725LNU329 pUC19c TOMATO Lycopersicum esculentum MD 3856, 4018, 4172, 4285299 726 LNU330 pUC19c TOMATO Lycopersicum esculentum MD 3857, 4019,4173, 4286 300 506 LNU331 Topo B TOMATO Lycopersicum esculentum MD 3858,4020, 4174, 4287 301 727 LNU332 pUC19c MAIZE Zea mays L. B73 4175, 4288302 508 LNU333 303 509 LNU335 Topo B WHEAT Triticum aestivum L. ND 3859,4021, 3859, 4021 304 728 LNU336 Topo B TOMATO Lycopersicum esculentum MD3860, 4022, 4176, 4289 305 729 LNU337 pUC19d GRAPE Vitis vinifera ND(redglob (red) x salt 3861, 4023, 4177, 4177 306 730 krik) LNU339 Topo BMAIZE Zea mays L. ND 3862, 4024, 4178, 4290 307 513 LNU340 pUC19c WHEATTriticum aestivum L. EYAL 3863, 4025, 3863, 4291 308 514 LNU341 309 515LNU342 Topo B TOMATO Lycopersicum esculentum MD 3864, 4026, 4179, 4292310 516 LNU343 Topo B WHEAT Triticum aestivum L. EYAL 4180, 4293 311 731LNU344 pUC19c WHEAT Triticum aestivum L. ND 3865, 4027 312 518 LNU345Topo B WHEAT Triticum aestivum L. EYAL 3866, 4028, 4181, 4294 313 519LNU346 pUC19c SORGHUM Sorghum bicolor ND 4182, 4295 314 520 LNU347pUC19c SORGHUM Sorghum bicolor ND 3867, 4029 315 521 LNU348 pUC19c MAIZEZea mays L. B73 3868, 4030 316 522 LNU349 pUC19c SOYBEAN Glycine max40-219 3869, 4031 317 523 LNU350 pUC19c WHEAT Triticum aestivum L. ND3870, 4032, 4183, 4296 318 732 LNU351 pUC19c WHEAT Triticum aestivum L.EYAL 3871, 4033, 4184, 4297 319 525 LNU352 pUC19c WHEAT Triticumaestivum L. EYAL 3872, 4034, 3872, 4298 320 526 LNU353 Topo B WHEATTriticum aestivum L. ND 3873, 4035, 4185, 4299 321 527 LNU354 pUC19cWHEAT Triticum aestivum L. EYAL 3874, 4036, 4186, 4300 322 528 LNU355pUC19d WHEAT Triticum aestivum L. EYAL 4187, 4301 323 529 LNU356 pUC19cTOMATO Lycopersicum esculentum MD 3875, 4037, 4188, 4302 324 530 LNU357pUC19c TOMATO Lycopersicum esculentum MD 3876, 4038, 3876, 4303 325 531LNU359 326 532 LNU360 Topo B MAIZE Zea mays L. B73 3877, 4039, 4189,4304 327 733 LNU361 pUC19c MAIZE Zea mays L. B73 4190, 4305 328 734LNU362 pUC19c RICE Oryza sativa L. Japonica Nipponbare 3878, 4040, 4191,4306 329 535 LNU363 Topo B RICE Oryza sativa L. Japonica Nipponbare3879, 4041, 4192, 4307 330 536 LNU364 pUC19c RICE Oryza sativa L.Japonica Nipponbare 3880, 4042, 4193, 4308 331 537 LNU365 pUC19c RICEOryza sativa L. Japonica Nipponbare 4194, 4309 332 538 LNU366 Topo BRICE Oryza sativa L. Japonica Nipponbare 4195, 4310 333 539 LNU367 TopoB RICE Oryza sativa L. Japonica Nipponbare 4196, 4311 334 540 LNU368pUC19c WHEAT Triticum aestivum L. ND 3881, 4043, 3881, 4312 335 735LNU369 pUC19c WHEAT Triticum aestivum L. ND 3882, 4044 336 542 LNU370pUC19c TOMATO Lycopersicum esculentum MD 3883, 4045, 3883, 4313 337 543LNU371 pUC19c MAIZE Zea mays L. B73 4197, 4314 338 736 LNU372 Topo BWHEAT Triticum aestivum L. ND 3884, 4046, 3884, 4315 339 737 LNU373pUC19c RICE Oryza sativa L. Indica Lebbonet 3885, 4047, 4198, 4316 340546 LNU374 pUC19c RICE Oryza sativa L. Japonica Nipponbare 3886, 4048,4199, 4317 341 547 LNU375 pUC19c TOMATO Lycopersicum esculentum MD 3887,4049, 4200, 4318 342 548 LNU376 pUC19c MAIZE Zea mays L. B73 3888, 4050,4201, 4319 343 549 LNU377 pUC19c SORGHUM Sorghum bicolor ND 3889, 4051,3889, 4320 344 550 LNU378 pUC19c WHEAT Triticum aestivum L. EYAL 3890,4052, 4202, 4052 235 738 LNU379 pUC19c SORGHUM Sorghum bicolor ND 3891,4053, 3891, 4321 345 552 LNU380 Topo B WHEAT Triticum aestivum L. ND3892, 4054 346 739 LNU381 pUC19c SORGHUM Sorghum bicolor ND 3893, 4055,3893, 4322 347 554 LNU382 pUC19c ARABIDOPSIS Arabidopsis thalianaKondara 3894, 4056, 4203, 4056 348 740 LNU383 pUC19c TOMATO Lycopersicumesculentum MD 3895, 4057, 4204, 4323 349 556 LNU384 Topo B TOMATOLycopersicum esculentum MD 3896, 4058, 4205, 4324 350 741 LNU385 Topo BRICE Oryza sativa L. Japonica Nipponbare 3897, 4059, 4206, 4325 351 558LNU386 pUC19c RICE Oryza sativa L. Indica Lebbonet 4207, 4326 352 559LNU387 pUC19c SORGHUM Sorghum bicolor ND 3898, 4060 353 742 LNU388 354561 LNU390 pUC19d TOMATO Lycopersicum esculentum MD 3899, 4061, 3899,4327 355 743 LNU391 pUC19c BARLEY Hordeum vulgare L. Manit 3900, 4062,4208, 4328 356 563 LNU392 pUC19c RICE Oryza sativa L. JaponicaNipponbare 3901, 4063, 4209, 4329 357 564 LNU393 pUC19c SORGHUM Sorghumbicolor ND 3902, 4064, 3902, 4330 358 744 LNU395 Topo B SORGHUM Sorghumbicolor ND 3903, 4065 359 566 LNU396 pUC19c SORGHUM Sorghum bicolor ND3904, 4066, 4210, 4331 360 567 LNU397 Topo B SORGHUM Sorghum bicolor ND4211, 4332 361 745 LNU399 pUC19c WHEAT Triticum aestivum L. EYAL 3905,4067, 4212, 4333 362 569 LNU401 pUC19c SORGHUM Sorghum bicolor ND 3906,4068, 4213, 4334 363 746 LNU402 pUC19c WHEAT Triticum aestivum L. ND3907, 4069, 3907, 4335 364 747 LNU403 pUC19c SORGHUM Sorghum bicolor ND3908, 4070 365 572 LNU405 pUC19c TOMATO Lycopersicum esculentum MD 3909,4071, 3909, 4336 366 748 LNU407 Topo B BARLEY Hordeum vulgare L. Manit3910, 4072, 4214, 4337 367 749 LNU408 pUC19c BARLEY Hordeum vulgare L.Spontaneum 3911, 4073 368 575 LNU409 Topo B BARLEY Hordeum vulgare L.Manit 3912, 4074, 3912, 4338 369 750 LNU410 pUC19c WHEAT Triticumaestivum L. ND 4215, 4339 370 577 LNU411 pUC19c TOMATO Lycopersicumesculentum MD 3913, 4075, 3913, 4340 371 578 LNU412 pUC19c COTTONGossypium barbadense Pima 3914, 4076, 4216, 4341 372 751 LNU413 pUC19cTOMATO Lycopersicum esculentum MD 4217, 4342 373 752 LNU414 pUC19c WHEATTriticum aestivum L. ND 3915, 4077 374 753 LNU415 pUC19c SORGHUM Sorghumbicolor ND 3916, 4078, 4218, 4343 375 582 LNU416 pUC19c MUSTARD Brassicajuncea ND 3917, 4079 376 754 LNU419 pUC19c TOMATO Lycopersicumesculentum MD 3918, 4080, 4219, 4344 377 755 LNU420 pUC19c SORGHUMSorghum bicolor ND 3919, 4081, 4220, 4345 378 586 LNU421 pUC19c SORGHUMSorghum bicolor ND 3920, 4082, 3920, 4346 379 756 LNU422 pUC19c SORGHUMSorghum bicolor ND 4221, 4347 380 588 LNU423 pUC19c SORGHUM Sorghumbicolor ND 4222, 4348 381 589 LNU424 pUC19c ARABIDOPSIS Arabidopsisthaliana Kondara 3921, 4083, 4223, 4349 382 590 LNU425 pUC19c BARLEYHordeum vulgare L. Manit 3922, 4084, 4224, 4350 383 591 LNU426 384 592LNU427 pUC19c RICE Oryza sativa L. Japonica Nipponbare 3923, 4085, 4225,4351 385 593 LNU429 pUC19c TOMATO Lycopersicum esculentum MD 3924, 4086,4226, 4086 386 594 LNU430 pUC19c TOMATO Lycopersicum esculentum MD 4227,4352 387 595 LNU432 pUC19c SORGHUM Sorghum bicolor ND 3925, 4087, 4228,4353 388 597 LNU433 Topo B SORGHUM Sorghum bicolor ND 3926, 4088, 4229,4354 389 598 LNU434 390 599 LNU435 Topo B BARLEY Hordeum vulgare L.Manit 3927, 4089, 4230, 4355 391 600 LNU436 Topo B BARLEY Hordeumvulgare L. Manit 3928, 4090 392 601 LNU437_H2 Topo B RICE Oryza sativaL. Japonica Nipponbare 3929, 4091, 4231, 4356 465 679 LNU438 pUC19cBARLEY Hordeum vulgare L. Manit 3930, 4092, 3930, 4357 393 603 LNU439pUC19c SORGHUM Sorghum bicolor ND 3931, 4093, 4232, 4358 394 757 LNU442pUC19c TOMATO Lycopersicum esculentum MD 3932, 4094, 4233, 4359 395 758LNU443 Topo B BRACHYPODIUM Brachypodiums distachyon 3933, 4095, 3933,4360 396 607 ND LNU444 pUC19c COTTON Gossypium barbadense Pima 3934,4096 397 759 LNU446 pUC19c SOYBEAN Glycine max 40-219 3935, 4097, 3935,4361 398 610 LNU447 pUC19c BARLEY Hordeum vulgare L. Manit 3936, 4098,3936, 4362 399 760 LNU448 pUC19c BARLEY Hordeum vulgare L. Spontaneum3937, 4099, 4234, 4363 400 761 LNU449 pUC19c COTTON Gossypium barbadensePima 3938, 4100 401 762 LNU450 pUC19c COTTON Gossypium barbadense Pima3939, 4101, 4235, 4364 402 763 LNU451 pUC19c TOMATO Lycopersicumesculentum MD 3940, 4102, 4236, 4365 403 615 LNU453 404 616 LNU454 TopoB TOMATO Lycopersicum esculentum MD 3941, 4103 405 764 LNU455 pUC19cTOMATO Lycopersicum esculentum MD 3942, 4104, 3942, 4366 406 618 LNU456pUC19c BARLEY Hordeum vulgare L. Manit 3943, 4105, 3943, 4367 407 619LNU458 pUC19c COTTON Gossypium barbadense Pima 3944, 4106, 3944, 4368408 621 LNU459 pUC19c MAIZE Zea mays L. B73 3945, 4107, 4237, 4369 409622 LNU460 pUC19c MAIZE Zea mays L. B73 3946, 4108, 3946, 4370 410 765LNU461 Topo B TOMATO Lycopersicum esculentum MD 3947, 4109, 4238, 4371411 766 LNU462 pUC19c TOMATO Lycopersicum esculentum MD 3948, 4110,4239, 4372 412 625 LNU463 pUC19c GRAPE Vitis vinifera ND(red glob (red)x salt 3949, 4111 413 767 krik) LNU464 414 627 LNU465 Topo B SORGHUMSorghum bicolor ND 3950, 4112, 3950, 4373 415 768 LNU466 416 629 LNU467pUC19c BARLEY Hordeum vulgare L. Spontaneum 3951, 4113, 4240, 4374 417630 LNU468 pUC19c TOMATO Lycopersicum esculentum MD 3952, 4114, 4241,4375 418 769 LNU469 pUC19c MAIZE Zea mays L. B73 3953, 4115, 3953, 4376419 632 LNU470 Topo B BARLEY Hordeum vulgare L. Spontaneum 3954, 4116,4242, 4377 420 770 LNU471 Topo B MAIZE Zea mays L. B73 3955, 4117, 4243,4378 421 771 LNU472 pUC19c BARLEY Hordeum vulgare L. Manit 3956, 4118,4244, 4379 422 772 LNU473 423 636 LNU474 pUC19c SOYBEAN Glycine max40-219 3957, 4119, 4245, 4119 424 773 LNU476 pUC19c MAIZE Zea mays L.B73 3958, 4120, 3958, 4380 425 774 LNU477 pUC19c SORGHUM Sorghum bicolorND 3959, 4121, 4246, 4381 426 639 LNU479 427 640 LNU480 Topo B SORGHUMSorghum bicolor ND 3960, 4122, 3960, 4382 428 641 LNU481 Topo B SORGHUMSorghum bicolor ND 3961, 4123, 4247, 4383 429 642 LNU482 Topo B COTTONGossypium barbadense Pima 3962, 4124, 4248, 4384 430 775 LNU483 Topo BRICE Oryza sativa L. Japonica Nipponbare 4249, 4385 431 644 LNU485pUC19c RICE Oryza sativa L. Japonica Nipponbare 3963, 4125, 4250, 4386432 776 LNU486 pUC19c RICE Oryza sativa L. Japonica Nipponbare 3964,4126 433 646 LNU487 pUC19c BARLEY Hordeum vulgare L. Manit 4251, 4387469 — LNU488 216 — LNU489 pUC19c TOMATO Lycopersicum esculentum MD 3965,4127, 4252, 4388 434 647 LNU490 435 648 LNU491 pUC19c SORGHUM Sorghumbicolor ND 3966, 4128, 4253, 4389 436 649 LNU492 pUC19c RICE Oryzasativa L. Japonica Nipponbare 3967, 4129 437 650 LNU493 pUC19c RICEOryza sativa L. Japonica Nipponbare 3968, 4130 438 651 LNU494 439 652LNU495 pUC19c SORGHUM Sorghum bicolor ND 3969, 4131, 3969, 4390 440 777LNU496 pUC19c WHEAT Triticum aestivum L. ND 3970, 4132, 4254, 4391 441778 LNU497 pUC19c WHEAT Triticum aestivum L. ND 3971, 4133, 4255, 4392442 655 LNU498 pUC19c SORGHUM Sorghum bicolor ND 3972, 4134, 4256, 4393443 656 LNU499 Topo B BARLEY Hordeum vulgare L. Manit 3973, 4135, 3973,4394 444 779 LNU500 pUC19c TOMATO Lycopersicum esculentum MD 3974, 4136445 658 LNU501 pUC19c SORGHUM Sorghum bicolor ND 3975, 4137, 3975, 4395446 659 LNU502 pUC19c BARLEY Hordeum vulgare L. Spontaneum 3976, 4138,3976, 4396 447 660 LNU503 pUC19c RICE Oryza sativa L. JaponicaNipponbare 3977, 4139 448 661 LNU504 pUC19c ARABIDOPSIS Arabidopsisthaliana Kondara 3978, 4140 449 780 LNU507 pUC19c BARLEY Hordeum vulgareL. Manit 3979, 4141 450 781 LNU508 Topo B RICE Oryza sativa L. JaponicaNipponbare 3980, 4142, 4257, 4257 451 665 LNU509 pUC19c RICE Oryzasativa L. Japonica Nipponbare 3981, 4143, 3981, 4397 452 666 LNU510 TopoB RICE Oryza sativa L. Japonica Nipponbare 3982, 4144, 4258, 4398 453667 LNU511 pUC19c RICE Oryza sativa L. Japonica Nipponbare 4259, 4399454 668 LNU512 pUC19c ARABIDOPSIS Arabidopsis thaliana Kondara 3983,4145, 4260, 4400 455 669 LNU513 pUC19c SOYBEAN Glycine max 40-219 3984,4146, 3984, 4401 456 782 LNU514 Topo B RICE Oryza sativa L. JaponicaNipponbare 3985, 4147, 3985, 4402 457 671 LNU517 pUC19c SOYBEAN Glycinemax 40-219 3986, 4148, 4261, 4403 458 783 LNU518 Topo B MAIZE Zea maysL. B73 3987, 4149 459 673 LNU519 Topo B MAIZE Zea mays L. B73 3988,4150, 4262, 4404 460 784 LNU520 Topo B SORGHUM Sorghum bicolor ND 3989,4151 461 675 LNU313 pUC19c SORGHUM Sorghum bicolor ND 4263, 4405 466 —LNU358 212 — LNU394 467 — LNU418 pUC19c MAIZE Zea mays L. B73 3990,4152, 3990, 4406 468 — Table 68. Provided are the genes which werecloned in high copy plasmids, along with the primers used for cloning,the organisms from which the genes were cloned and the resultingpolynucleotide (“polyn.”) and polypeptide (“polyp.”) sequences of thecloned gene.

Example 14 Transforming Agrobacterium tumefaciens Cells with BinaryVectors Harboring Putative Genes

Each of the binary vectors described in Example 13 above were used totransform Agrobacterium cells. Two additional binary constructs, havingonly the At6669, or the RootP promoter or no additional promoter wereused as negative controls.

The binary vectors were introduced to Agrobacterium tumefaciens GV301,or LB4404 competent cells (about 10⁹ cells/mL) by electroporation. Theelectroporation was performed using a MicroPulser electroporator(Biorad), 0.2 cm cuvettes (Biorad) and EC-2 electroporation program(Biorad). The treated cells were cultured in LB liquid medium at 28° C.for 3 hours, then plated over LB agar supplemented with gentamycin (50mg/L; for Agrobacterium strains GV301) or streptomycin (300 mg/L; forAgrobacterium strain LB4404) and kanamycin (50 mg/L) at 28° C. for 48hours. Agrobacterium colonies, which were developed on the selectivemedia, were further analyzed by PCR using the primers designed to spanthe inserted sequence in the pPI plasmid. The resulting PCR productswere isolated and sequenced as described in Example 13 above, to verifythat the correct polynucleotide sequences of the invention are properlyintroduced to the Agrobacterium cells.

Example 15 Transformation of Arabidopsis thaliana Plants with thePolynucleotides of the Invention

Arabidopsis thaliana Columbia plants (T₀ plants) were transformed usingthe Floral Dip procedure described by Clough and Bent, 1998 (Floral dip:a simplified method for Agrobacterium-mediated transformation ofArabidopsis thaliana. Plant J 16:735-43) and by Desfeux et al., 2000(Female Reproductive Tissues Are the Primary Target ofAgrobacterium-Mediated Transformation by the Arabidopsis Floral-DipMethod. Plant Physiol, July 2000, Vol. 123, pp. 895-904), with minormodifications. Briefly, To Plants were sown in 250 ml pots filled withwet peat-based growth mix. The pots were covered with aluminum foil anda plastic dome, kept at 4° C. for 3-4 days, then uncovered and incubatedin a growth chamber at 18-24° C. under 16/8 hour light/dark cycles. TheT₀ plants were ready for transformation six days before anthesis.

Single colonies of Agrobacterium carrying the binary constructs, weregenerated as described in Examples 13 and 14 above. Colonies werecultured in LB medium supplemented with kanamycin (50 mg/L) andgentamycin (50 mg/L). The cultures were incubated at 28° C. for 48 hoursunder vigorous shaking and then centrifuged at 4000 rpm for 5 minutes.The pellets comprising the Agrobacterium cells were re-suspended in atransformation medium containing half-strength (2.15 g/L)Murashige-Skoog (Duchefa); 0.044 μM benzylamino purine (Sigma); 112 μg/LB5 Gambourg vitamins (Sigma); 5% sucrose; and 0.2 ml/L Silwet L-77 (OSISpecialists, CT) in double-distilled water, at pH of 5.7.

Transformation of T₀ plants was performed by inverting each plant intoan Agrobacterium suspension, such that the above ground plant tissue wassubmerged for 3-5 seconds. Each inoculated T₀ plant was immediatelyplaced in a plastic tray, then covered with clear plastic dome tomaintain humidity and was kept in the dark at room temperature for 18hours, to facilitate infection and transformation. Transformed(transgenic) plants were then uncovered and transferred to a greenhousefor recovery and maturation. The transgenic T₀ plants were grown in thegreenhouse for 3-5 weeks until siliques are brown and dry. Seeds wereharvested from plants and kept at room temperature until sowing.

For generating T₁ and T₂ transgenic plants harboring the genes, seedscollected from transgenic T₀ plants were surface-sterilized by soakingin 70% ethanol for 1 minute, followed by soaking in 5% sodiumhypochloride and 0.05% triton for 5 minutes. The surface-sterilizedseeds were thoroughly washed in sterile distilled water then placed onculture plates containing half-strength Murashige-Skoog (Duchefa); 2%sucrose; 0.8% plant agar; 50 mM kanamycin; and 200 mM carbenicylin(Duchefa). The culture plates were incubated at 4° C. for 48 hours, thentransferred to a growth room at 25° C. for an additional week ofincubation. Vital T₁ Arabidopsis plants were transferred to freshculture plates for another week of incubation. Following incubation theT₁ plants were removed from culture plates and planted in growth mixcontained in 250 ml pots. The transgenic plants were allowed to grow ina greenhouse to maturity. Seeds harvested from T₁ plants were culturedand grown to maturity as T₂ plants under the same conditions as used forculturing and growing the T₁ plants.

Example 16 Evaluating Transgenic Arabidopsis NUE Under Low or NormalNitrogen Conditions Using In Vitro (Tissue Culture) Assays

Assay 1: Plant Growth Under Low and Favorable Nitrogen ConcentrationLevels

Surface sterilized seeds were sown in basal media [50% Murashige-Skoogmedium (MS) supplemented with 0.8% plant agar as solidifying agent] inthe presence of Kanamycin (used as a selecting agent). After sowing,plates were transferred for 2-3 days for stratification at 4° C. andthen grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7to 10 days. At this time point, seedlings randomly chosen were carefullytransferred to plates containing ½ MS media (15 mM N) for the normalnitrogen concentration treatment and 0.75 mM nitrogen for the lownitrogen concentration treatments. For experiments performed in T₂lines, each plate contained 5 seedlings of the same transgenic event,and 3-4 different plates (replicates) for each event. For eachpolynucleotide of the invention at least four-five independenttransformation events were analyzed from each construct. For experimentsperformed in T₁ lines, each plate contained 5 seedlings of 5 independenttransgenic events and 3-4 different plates (replicates) were planted. Intotal, for T₁ lines, 20 independent events were evaluated. Plantsexpressing the polynucleotides of the invention were compared to theaverage measurement of the control plants (empty vector or GUS reportergene under the same promoter) used in the same experiment.

Digital imaging—A laboratory image acquisition system, which consists ofa digital reflex camera (Canon EOS 300D) attached with a 55 mm focallength lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which includes 4 light units (4×150 Watts light bulb) andlocated in a darkroom, is used for capturing images of plantlets sawn inagar plates.

The image capturing process is repeated every 3-4 days starting at day 1till day 10. An image analysis system was used, which consists of apersonal desktop computer (Intel P4 3.0 GHz processor) and a publicdomain program—ImageJ 1.39 [Java based image processing program whichwas developed at the U.S. National Institutes of Health and freelyavailable on the internet at Hypertext Transfer Protocol://rsbweb (dot)nih (dot) gov/]. Images were captured in resolution of 10 Mega Pixels(3888×2592 pixels) and stored in a low compression JPEG (JointPhotographic Experts Group standard) format. Next, analyzed data wassaved to text files and processed using the JMP statistical analysissoftware (SAS institute).

Seedling analysis—Using the digital analysis seedling data wascalculated, including leaf area, root coverage and root length.

The relative growth rate for the various seedling parameters wascalculated according to the following Formulas VI (RGR of leaf area,above), XVIII (RGR root length, below) and Formula VII (RGR of rootcoverage, above).

Relative growth rate of root length=Regression coefficient of rootlength along time course.  Formula XVIII—

At the end of the experiment, plantlets were removed from the media andweighed for the determination of plant fresh weight. Plantlets were thendried for 24 hours at 60° C., and weighed again to measure plant dryweight for later statistical analysis. Growth rate was determined bycomparing the leaf area coverage, root coverage and root length, betweeneach couple of sequential photographs, and results are used to resolvethe effect of the gene introduced on plant vigor under optimalconditions. Similarly, the effect of the gene introduced on biomassaccumulation, under optimal conditions, was determined by comparing theplants' fresh and dry weight to that of control plants (containing anempty vector or the GUS reporter gene under the same promoter). Fromevery construct created, 3-5 independent transformation events areexamined in replicates.

Statistical analyses—To identify genes conferring significantly improvedplant vigor or enlarged root architecture, the results obtained from thetransgenic plants were compared to those obtained from control plants.To identify outperforming genes and constructs, results from theindependent transformation events tested were analyzed separately. Toevaluate the effect of a gene event over a control the data was analyzedby Student's t-test and the p value is calculated. Results wereconsidered significant if p≤0.1. The JMP statistics software package wasused (Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results:

The genes presented in the following Tables were cloned under theregulation of a constitutive promoter (At6669). The evaluation of eachgene was carried out by testing the performance of different number ofevents. Some of the genes were evaluated in more than one tissue cultureassay. The results obtained in these second experiments weresignificantly positive as well. The evaluation of each gene wasperformed by testing the performance of different number of events.Event with p-value<0.1 was considered statistically significant.

The genes presented in Tables 69-72 showed a significant improvement inplant NUE since they produced larger plant biomass (plant fresh and dryweight; leaf area, root length and root coverage) in T2 generation(Tables 69-70) or T1 generation (Tables 71-72) when grown under limitingnitrogen growth conditions, compared to control plants. Plants producinglarger root biomass have better possibilities to absorb larger amount ofnitrogen from soil.

TABLE 69 Genes showing improved plant performance at nitrogen deficientconditions (T2 generation) Dry Weight [mg] Fresh Weight [mg] % P- % GeneName Event # P-Ave. Val. Incr. Ave. Val. Incr. LNU437_H2 66104.1 5.20.02 43 79.4 0.17 21 LNU437_H2 66104.2 4.7 0.29 29 — — — LNU437_H266104.3 4.8 0.08 31 81.4 0.18 24 LNU437_H2 66105.3 4.8 0.11 31 93.8 0.1343 LNU426 66147.3 6.6 L 80 120.6 L 84 LNU420 64008.4 5.0 0.09 36 — — —LNU352 64199.1 6.3 L 71 106.9 L 63 LNU292 64085.4 4.8 0.08 31 87.7 0.0534 CONT. — 3.7 — — 65.5 — — LNU483 64803.2 7.5 0.07 33 153.0 L 42 LNU48364805.1 8.6 0.03 52 139.3 L 29 LNU483 64805.2 6.5 0.09 16 137.8 L 28LNU483 64806.2 7.3 0.12 29 141.4 0.04 31 LNU477 63886.1 6.4 0.07 13128.0 0.07 19 LNU477 63888.1 6.4 0.20 14 123.2 0.18 14 LNU464 65076.46.4 0.27 13 138.2 0.09 28 LNU447 65000.4 7.2 0.14 28 138.2 0.10 28LNU447 65002.2 6.4 0.18 13 138.8 0.07 29 LNU447 65002.3 — — — 119.3 0.2711 LNU439 64616.2 7.6 0.04 35 148.7 0.11 38 LNU439 64616.3 8.2 0.07 45152.3 0.04 41 LNU439 64618.3 7.1 0.11 26 134.1 0.11 24 LNU425 63910.9 —— — 119.3 0.25 11 LNU425 63911.9 8.7 0.04 55 172.5 0.03 60 LNU41464475.1 7.8 0.02 39 138.4 L 28 LNU414 64479.1 8.1 0.02 44 150.5 L 40LNU414 64480.2 7.8 0.12 38 152.4 0.09 41 LNU346 65008.2 7.1 0.14 26136.5 0.01 27 LNU336 64447.2 — — — 135.7 0.12 26 LNU336 64448.2 8.4 0.1050 163.0 0.03 51 LNU336 64448.3 6.3 0.05 12 126.5 0.01 17 LNU336 64449.37.4 0.03 31 135.3 0.25 26 CONT. — 5.6 — — 107.8 — — LNU473 65770.4 5.40.13 21 102.8 0.26 18 LNU470 64229.1 6.0 0.13 33 123.1 0.13 41 LNU46064359.4 — — — 95.3 0.26  9 LNU421 64303.3 6.0 0.19 35 136.2 0.06 56LNU421 64304.4 — — — 95.8 0.24 10 LNU408 64248.10 5.2 0.28 16 108.7 0.2925 LNU408 64250.8 6.0 0.17 35 125.6 0.25 44 LNU380 65765.3 — — — 109.10.04 25 LNU340 64290.7 6.1 0.17 36 124.9 0.08 43 LNU331 64212.1 — — —111.9 0.25 29 LNU331 64214.2 — — — 122.7 0.04 41 LNU331 64215.1 8.0 L 78156.9 L 80 LNU306 64132.6 — — — 99.3 0.21 14 CONT. — 4.5 — — 87.1 — —LNU456 63991.8 — — — 85.1 0.26 23 LNU456 63992.6 — — — 106.8 0.06 55LNU430 63934.3 6.4 0.04 67 129.1 0.04 87 LNU430 63952.1 — — — 81.3 0.2718 LNU412 63940.1 — — — 93.1 0.08 35 LNU412 63940.12 — — — 84.8 0.08 23LNU412 63940.8 5.3 0.29 39 — — — LNU407 64218.1 4.9 0.12 28 96.2 0.14 40LNU407 64218.2 — — — 93.1 0.11 35 LNU407 64219.2 6.9 L 79 115.7 L 68LNU384 64161.1 — — — 78.9 0.27 14 LNU384 64161.3 — — — 91.2 0.03 32LNU384 64161.7 — — — 82.2 0.13 19 LNU360 64029.3 — — — 91.5 0.11 33LNU335 64168.18 — — — 82.2 0.22 19 LNU335 64169.2 5.5 0.10 44 113.0 0.0964 LNU301 63927.3 — — — 167.8 0.01 143  LNU301 63927.5 — — — 86.0 0.1225 LNU301 63950.3 7.2 0.02 88 136.0 L 97 CONT. — 3.8 — — 68.9 — — LNU45063708.3 6.8 0.14 34 136.4 0.05 63 LNU450 63710.2 6.3 0.01 24 122.7 L 46LNU450 63712.3 7.0 L 39 117.8 0.12 40 LNU429 63937.4 — — — 102.1 0.03 22LNU416 64134.2 — — — 95.7 0.10 14 LNU416 64136.4 7.3 0.05 45 132.1 0.0158 LNU412 63940.12 — — — 136.8 0.15 63 LNU412 63940.8 — — — 114.6 0.2037 LNU359 66154.5 — — — 97.3 0.06 16 LNU359 66154.6 — — — 106.3 0.16 27LNU349 63990.4 — — — 101.5 0.02 21 LNU293 65048.1 — — — 109.3 L 30LNU293 65050.3 — — — 97.5 0.11 16 LNU293 65051.3 — — — 120.5 0.14 44CONT. — 5.1 — — 83.8 — — LNU498 64185.3 5.1 0.13 32 119.0 0.15 28 LNU49364190.3 — — — 108.9 0.26 17 LNU493 64191.4 — — — 113.4 0.29 22 LNU45564187.5 — — — 109.8 0.30 18 LNU343 64208.4 4.4 0.13 15 — — — LNU32263918.1 4.6 0.09 21 — — — LNU305 64111.2 4.3 0.26 13 — — — CONT. — 3.8 —— 93.1 — — LNU487 64706.2 4.8 0.02 22 — — — LNU465 64020.1 4.6 0.07 1784.2 0.23 13 LNU446 64546.2 4.6 0.26 18 91.9 0.13 23 LNU446 64546.3 5.10.18 28 — — — LNU443 64023.2 5.0 0.18 26 82.5 0.29 11 LNU443 64024.3 6.4L 62 — — — LNU436 64240.1 4.9 0.03 24 — — — LNU436 64240.2 5.4 0.10 36103.7 0.09 39 LNU436 64242.2 5.6 L 43 — — — LNU379 64170.2 4.9 0.28 2395.0 0.22 27 LNU315 64224.1 4.8 0.09 20 82.9 0.28 11 LNU315 64224.3 5.80.07 47 104.6 L 40 LNU315 64225.1 4.8 0.02 22 88.2 0.16 18 LNU31564227.3 4.4 0.28 11 — — — CONT. — 4.0 — — 74.6 — — LNU449 63890.1 8.10.25 24 — — — LNU449 64571.3 9.1 0.23 39 188.3 0.13 43 LNU435 64464.310.4  0.04 57 220.1 L 67 LNU432 64559.2 9.9 0.19 50 201.3 0.21 53 LNU36764398.2 8.9 0.23 35 195.4 0.13 48 LNU362 64324.3 — — — 148.4 0.25 13CONT. — 6.6 — — 131.6 — — LNU495 64697.2 — — — 99.4 0.03 63 LNU49564697.3 — — — 114.5 0.13 87 LNU495 64698.2 4.7 0.26 14 95.0 L 55 LNU49564701.3 — — — 99.1 0.03 62 LNU487 64702.1 — — — 89.5 0.26 46 LNU48764702.3 — — — 77.1 0.28 26 LNU487 64704.2 — — — 106.3 0.19 74 LNU48764705.4 — — — 107.3 L 75 LNU487 64706.2 — — — 93.0 0.07 52 LNU47464379.1 — — — 88.2 0.13 44 LNU474 64381.1 — — — 84.8 0.11 39 LNU47464382.3 — — — 88.9 0.03 45 LNU474 64383.2 — — — 92.4 0.03 51 LNU46564020.1 — — — 96.3 0.06 58 LNU465 64020.4 — — — 80.2 0.11 31 LNU46564021.3 — — — 73.8 0.29 21 LNU465 64021.7 — — — 93.1 0.04 52 LNU44664546.2 — — — 77.5 0.19 27 LNU446 64546.3 — — — 123.7 0.12 102  LNU44664548.1 — — — 82.4 0.08 35 LNU446 64548.2 — — — 101.7 0.29 66 LNU44664549.3 — — — 93.5 0.01 53 LNU443 64023.2 — — — 91.8 0.01 50 LNU44364023.9 — — — 72.1 0.27 18 LNU436 64240.1 — — — 96.7 0.02 58 LNU43664240.2 — — — 98.0 0.06 60 LNU436 64241.3 5.6 0.22 36 124.6 0.02 104 LNU436 64242.2 — — — 102.4 0.13 68 LNU436 64243.1 5.0 0.26 23 134.4 0.06120  LNU379 64170.2 — — — 74.5 0.19 22 LNU379 64170.3 — — — 76.5 0.15 25LNU379 64172.1 — — — 84.0 0.11 37 LNU379 64172.2 — — — 77.3 0.14 26LNU315 64224.1 — — — 105.7 0.02 73 LNU315 64225.2 5.3 0.09 29 139.4 0.04128  LNU315 64226.3 5.2 0.26 26 103.4 0.06 69 LNU315 64227.3 5.2 L 27110.2 L 80 CONT. — 4.1 — — 61.1 — — LNU520 64156.7 6.1 0.16 22 114.80.25 15 LNU405 64158.9 6.9 0.14 39 138.8 L 39 LNU403 64239.1 6.7 0.26 33125.2 0.18 26 CONT. — 5.0 — — 99.7 — — LNU519 64678.1 4.0 0.30 16 — — —LNU519 64679.1 — — — 66.8 0.25 11 LNU519 64681.8 — — — 76.5 0.13 27LNU500 64220.1 — — — 74.8 0.02 24 LNU500 64223.1 4.0 0.29 16 82.8 0.0937 LNU459 64542.3 — — — 83.5 0.08 38 LNU348 64472.2 4.1 0.17 19 86.70.04 44 LNU348 64474.1 — — — 71.9 0.11 19 LNU348 64474.2 — — — 83.8 0.1439 LNU329 63428.2 — — — 68.7 0.19 14 LNU329 63429.1 — — — 70.4 0.16 17CONT. — 3.4 — — 60.4 — — LNU499 64146.11 4.5 0.06 27 — — — LNU49066095.2 4.1 0.05 17 — — — LNU437_H2 66104.1 5.5 L 54 98.0 L 40 LNU437_H266104.2 4.3 0.06 23 — — — LNU433 64814.1 4.9 0.20 39 — — — LNU43364816.1 4.9 0.08 39 — — — LNU433 64817.5 4.0 0.14 15 — — — LNU41664134.1 4.3 0.01 22 79.6 0.21 14 LNU416 64134.11 4.4 L 25 76.8 0.22 10LNU416 64134.2 4.5 0.16 27 90.6 0.02 30 LNU395 64142.5 4.8 0.02 34 83.40.28 19 LNU395 64143.6 5.5 0.12 56 94.2 0.19 35 LNU312 64000.1 4.8 L 34— — — LNU312 64000.2 4.2 0.05 20 — — — LNU312 64002.2 4.5 0.14 26 — — —LNU312 64002.3 4.3 0.13 22 — — — LNU312 64002.5 6.0 L 69 96.8 0.01 39LNU311 66099.1 4.5 0.18 27 — — — LNU311 66100.3 4.4 0.14 25 88.2 0.23 26CONT. — 3.5 — — 69.8 — — LNU468 63491.1 — — — 143.7 0.04 24 LNU46763718.2 — — — 152.4 0.21 32 LNU347 63510.2 7.4 0.05 16 145.8 0.10 26LNU347 63513.3 9.3 0.06 45 178.1 L 54 CONT. — 6.4 — — 115.5 — — LNU49764207.2 6.2 0.05 47 119.8 0.07 31 LNU491 64404.3 5.5 0.27 29 122.7 0.2434 LNU491 64404.6 5.5 0.03 29 108.6 0.17 19 LNU449 63890.1 4.8 0.26 13 —— — LNU449 63892.1 5.6 0.07 32 125.4 0.04 37 LNU432 64066.2 6.0 0.23 42122.2 0.22 34 CONT. — 4.2 — — 91.5 — — LNU438 63994.5 7.7 0.14 24 — — —LNU354 63970.7 8.0 0.15 27 — — — LNU295 63899.5 7.6 0.13 22 — — — CONT.— 6.2 — — — — — LNU483 64803.2 5.0 0.13 24 — — — LNU483 64803.3 5.9 0.0745 125.1 0.13 31 LNU483 64805.2 5.3 0.05 30 — — — LNU414 64475.1 4.70.18 16 — — — LNU378 64494.2 5.1 0.13 25 — — — LNU364 64441.3 4.9 0.0921 — — — LNU346 65008.2 5.9 0.08 44 — — — CONT. — 4.1 — — 95.3 — —LNU510 64152.1 — — — 80.1 0.12 15 LNU510 64154.2 — — — 101.7 0.27 46LNU438 63994.12 — — — 83.5 0.27 20 LNU354 63970.7 4.8 0.28 14 84.6 0.2321 LNU310 63904.1 — — — 77.8 0.27 12 LNU295 63899.5 — — — 79.2 0.28 14LNU295 63899.8 6.1 0.01 45 109.6 0.03 57 CONT. — 4.2 — — 69.8 — — LNU49066092.3 — — — 76.4 0.18 23 LNU443 64024.4 4.3 0.18 43 97.2 0.08 57LNU443 64024.7 4.6 0.04 50 95.4 0.11 54 LNU439 64616.2 4.2 0.12 36 80.70.13 30 LNU439 64618.3 4.9 0.03 60 95.2 0.04 54 LNU437_H2 66104.1 3.90.15 29 78.8 0.15 27 LNU436 64240.2 4.3 0.05 41 85.2 0.04 38 LNU43664242.2 4.2 0.07 37 79.9 0.09 29 LNU436 64243.1 3.9 0.21 28 83.4 0.17 35LNU433 64815.1 4.8 0.11 56 95.2 0.08 54 LNU433 64815.2 4.6 0.15 52 88.20.09 43 LNU433 64816.1 5.8 L 90 97.0 0.03 57 LNU298 66089.1 — — — 73.50.25 19 LNU293 65050.3 3.8 0.20 24 — — — LNU293 65051.3 5.0 0.08 63 91.20.05 47 CONT. — 3.0 — — 61.9 — — Table 69: “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant.

TABLE 70 Genes showing improved plant performance at nitrogen deficientconditions (T2 generation) Roots Coverage Leaf Area [cm²] [cm²] RootsLength [cm²] P- % P- % P- % Gene Name Event # Ave. Val. Incr. Ave. Val.Incr. Ave. Val. Incr. LNU437_H2 66104.1 0.4 0.05 28 9.0 0.10 26 — — —LNU437_H2 66104.2 0.5 0.16 34 — — — — — — LNU437_H2 66104.3 0.5 0.02 42— — — — — — LNU437_H2 66105.3 0.5 0.02 43 10.1 0.05 40 — — — LNU42666147.3 0.6 L 72 10.0 0.03 38 — — — LNU420 64006.3 — — — — — — 7.4 0.168 LNU420 64007.3 0.4 0.07 26 9.1 0.06 27 7.6 0.04 10 LNU352 64199.1 0.6L 88 12.9 L 79 8.1 L 17 LNU352 64200.1 0.5 0.04 37 — — — — — — LNU35264200.10 — — — — — — 7.3 0.21 6 LNU352 64200.4 0.4 0.06 32 10.0 0.03 397.6 0.07 10 LNU292 64084.1 — — — — — — 7.3 0.22 5 LNU292 64085.4 0.40.15 20 8.5 0.21 18 7.6 0.10 10 CONT. — 0.3 — — 7.2 — — 6.9 — — LNU48364803.2 — — — 17.0 0.01 38 — — — LNU483 64803.3 — — — 15.9 0.08 30 — — —LNU483 64805.1 — — — 21.9 L 79 7.8 L 9 LNU483 64805.2 — — — 20.9 L 71 —— — LNU483 64806.2 — — — 17.4 0.08 42 — — — LNU477 63889.2 — — — — — —7.4 0.22 3 LNU464 65073.1 — — — — — — 7.7 0.02 7 LNU464 65076.1 — — — —— — 7.6 0.02 6 LNU464 65076.4 0.7 0.29 10 — — — — — — LNU447 65000.1 — —— — — — 7.4 0.22 4 LNU447 65002.3 — — — 13.1 0.18  7 — — — LNU43964616.2 0.7 0.24 16 — — — — — — LNU439 64616.3 0.8 0.05 17 14.2 0.13 16— — — LNU439 64618.3 0.7 0.26  8 — — — — — — LNU425 63911.9 0.8 L 19 — —— 7.5 0.22 4 LNU414 64475.1 0.7 0.09  7 — — — — — — LNU414 64479.1 0.8 L21 — — — — — — LNU414 64480.2 0.8 0.14 23 — — — 7.5 0.13 5 LNU34665007.3 — — — — — — 7.4 0.18 4 LNU346 65008.2 0.7 L 15 — — — 7.5 0.06 5LNU346 65009.2 — — — — — — 7.5 0.13 4 LNU336 64448.2 0.9 0.03 34 14.00.23 14 — — — LNU336 64448.3 0.7 0.26  6 — — — — — — CONT. — 0.6 — —12.2 — — 7.2 — — LNU473 65770.4 0.6 0.23 12 12.8 0.25  9 7.5 0.17 5LNU470 64228.3 — — — 13.7 0.06 16 — — — LNU470 64229.1 0.7 0.06 27 14.40.06 22 7.9 0.03 9 LNU460 64359.3 — — — 14.8 0.20 25 8.0 0.04 12 LNU46064361.4 0.6 0.24 13 — — — — — — LNU460 64362.1 — — — 13.4 0.14 13 — — —LNU421 64302.7 0.6 0.25 12 13.3 0.02 13 7.8 0.01 9 LNU421 64303.3 0.70.12 26 — — — 7.6 0.28 5 LNU421 64304.4 — — — — — — 7.6 0.06 6 LNU42164305.11 — — — — — — 7.9 0.01 10 LNU408 64248.10 0.6 0.09 19 13.6 0.1015 7.4 0.22 4 LNU408 64248.12 — — — — — — 7.6 0.07 5 LNU408 64248.16 — —— — — — 7.8 0.07 9 LNU380 65764.2 — — — — — — 7.4 0.10 3 LNU380 65764.3— — — 13.2 0.15 12 7.4 0.22 3 LNU380 65765.4 — — — — — — 7.4 0.23 3LNU340 64290.7 0.6 0.10 23 15.4 0.03 30 8.1 L 13 LNU340 64291.10 — — — —— — 7.8 L 8 LNU340 64292.5 — — — 13.1 0.25 11 7.8 L 9 LNU331 64212.1 — —— — — — 7.6 0.07 6 LNU331 64212.3 — — — — — — 7.7 0.10 7 LNU331 64214.20.7 0.03 29 14.1 0.02 19 7.8 0.04 9 LNU331 64215.1 0.8 L 46 17.1 L 448.1 0.03 13 LNU331 64215.3 — — — — — — 7.4 0.16 3 CONT. — 0.5 — — 11.8 —— 7.2 — — LNU456 63991.8 — — — — — — 7.7 0.23 7 LNU430 63934.3 0.5 0.0720 — — — — — — LNU430 63936.2 0.5 0.20 12 — — — 7.7 0.24 6 LNU40764218.1 — — — 10.2 0.26 12 — — — LNU407 64219.1 — — — 10.4 0.11 15 7.90.11 8 LNU407 64219.2 0.6 0.05 35 — — — — — — LNU402 63914.2 0.5 0.14 1611.5 0.15 27 8.1 0.07 12 LNU360 64029.3 — — — 10.6 0.23 17 — — — LNU33564168.15 — — — — — — 8.0 0.10 11 LNU335 64169.2 0.5 0.04 21 — — — — — —LNU301 63927.3 0.6 0.19 40 — — — — — — LNU301 63950.3 0.6 L 57 13.0 0.0344 — — — CONT. — 0.4 — — 9.0 — — 7.3 — — LNU450 63708.3 0.7 0.02 32 14.50.10 20 7.7 0.22 7 LNU450 63710.2 0.7 0.03 22 — — — 7.7 0.21 7 LNU45063712.3 0.7 0.04 28 14.7 0.03 22 7.5 0.26 5 LNU429 63937.4 — — — — — —8.1 0.01 12 LNU429 63938.2 — — — — — — 7.6 0.25 5 LNU416 64134.2 0.60.22  6 — — — 7.5 0.29 4 LNU416 64136.4 0.7 0.03 20 15.4 0.04 27 7.80.05 9 LNU412 63940.12 0.7 0.27 25 — — — — — — LNU412 63940.8 0.7 0.2018 — — — 7.6 0.27 6 LNU359 66154.6 0.7 L 20 — — — 7.5 0.29 4 LNU34963989.5 — — — — — — 7.5 0.28 4 LNU349 63990.2 — — — — — — 7.5 0.24 5LNU293 65048.1 0.6 0.25  8 — — — — — — LNU293 65051.3 0.7 0.21 20 — — —— — — CONT. — 0.6 — — 12.1 — — 7.2 — — LNU498 64184.3 — — — 9.4 0.06 257.3 0.12 10 LNU498 64186.1 — — — 8.3 0.07 11 — — — LNU498 64186.2 — — —9.9 L 33 7.4 0.02 11 LNU498 64186.3 — — — 8.5 0.02 14 7.3 0.04 9 LNU49364190.1 — — — 9.8 L 31 7.3 0.01 10 LNU493 64190.3 — — — 9.4 0.02 26 7.00.24 4 LNU493 64191.2 0.5 0.14 20 9.6 0.07 28 7.1 0.19 7 LNU493 64191.3— — — — — — 7.4 L 11 LNU493 64191.4 — — — — — — 7.2 0.15 8 LNU45564187.5 0.5 0.06 16 10.1 L 34 7.5 0.05 12 LNU455 64189.4 — — — 9.9 0.0133 7.2 0.05 7 LNU455 64189.7 — — — — — — 7.0 0.19 5 LNU343 64208.4 — — —8.5 0.29 14 7.3 0.02 10 LNU328 64150.1 — — — 9.4 0.06 26 7.4 0.07 11LNU328 64150.2 — — — 9.4 0.19 26 7.0 0.21 5 LNU328 64150.4 — — — 8.60.02 15 7.3 0.01 10 LNU328 64151.1 — — — 8.9 L 19 7.2 0.11 8 LNU32263917.2 — — — 9.5 0.01 28 7.3 0.02 10 LNU322 63918.1 0.5 0.05 15 9.10.02 21 — — — LNU322 63918.4 — — — — — — 7.1 0.11 7 LNU317 64097.2 — — —9.0 0.12 20 7.5 0.03 12 LNU305 64114.1 — — — — — — 7.2 0.05 8 LNU30564115.1 — — — 9.4 0.22 25 7.3 0.02 9 CONT. — 0.4 — — 7.5 — — 6.7 — —LNU495 64697.3 — — — — — — 7.6 0.06 6 LNU495 64701.3 — — — 10.2 0.10 11— — — LNU487 64702.3 — — — — — — 7.6 L 7 LNU487 64706.2 0.5 0.25  8 — —— — — — LNU474 64381.1 — — — — — — 7.5 0.25 5 LNU474 64383.2 — — — — — —7.5 0.19 5 LNU465 64020.1 0.5 0.12 17 — — — — — — LNU465 64020.4 0.50.19 18 10.5 0.30 14 — — — LNU446 64546.2 0.5 0.16 15 13.6 0.02 48 7.50.05 6 LNU446 64546.3 — — — — — — 7.3 0.24 2 LNU446 64548.2 — — — — — —7.6 0.20 6 LNU446 64549.2 — — — 10.8 0.11 18 7.9 0.03 11 LNU443 64023.2— — — 11.6 0.09 26 7.8 L 9 LNU443 64023.9 — — — — — — 7.7 0.07 8 LNU44364024.7 — — — 10.3 0.16 13 7.6 0.14 7 LNU436 64240.1 0.6 0.18 23 — — —7.5 0.09 5 LNU436 64240.2 — — — 11.2 0.28 23 — — — LNU436 64243.1 0.6 L21 10.9 0.07 19 7.6 0.05 6 LNU379 64170.2 — — — 12.0 0.20 31 — — —LNU379 64170.4 — — — — — — 7.7 0.12 8 LNU315 64224.3 0.5 0.06 16 13.1 L43 7.6 0.07 7 LNU315 64225.1 0.5 0.28 11 — — — — — — CONT. — 0.5 — — 9.2— — 7.1 — — LNU497 64206.2 — — — — — — 7.1 0.27 7 LNU497 64207.3 — — — —— — 7.2 0.14 9 LNU491 64404.3 — — — — — — 6.9 0.18 4 LNU491 64405.2 0.70.13 31 11.2 0.16 21 7.4 0.01 13 LNU491 64406.4 0.7 0.08 31 12.8 0.05 387.9 0.01 20 LNU449 64570.1 — — — — — — 7.4 0.07 12 LNU449 64571.3 0.70.03 35 13.7 0.13 48 7.5 0.01 14 LNU435 64464.3 0.9 0.04 66 13.1 0.08 417.4 0.02 13 LNU432 64065.2 0.6 0.24  8 — — — 7.2 L 10 LNU432 64559.2 0.7L 30 13.1 0.04 41 7.5 L 15 LNU432 64560.3 0.6 0.26 13 — — — — — — LNU43264560.5 0.6 0.21 17 12.2 0.24 32 6.9 0.27 5 LNU378 64493.3 — — — — — —7.0 0.28 6 LNU378 64495.2 — — — — — — 7.1 0.17 8 LNU378 64495.4 — — — —— — 7.4 0.07 12 LNU367 64397.1 0.6 0.28 10 — — — — — — LNU367 64398.20.7 0.19 28 12.2 0.20 32 7.4 0.04 13 LNU367 64399.1 — — — — — — 7.4 0.0513 LNU364 64441.2 — — — 12.0 0.26 29 7.3 0.19 11 LNU362 64323.1 — — — —— — 7.0 0.06 6 LNU362 64324.3 0.6 0.20 20 — — — — — — CONT. — 0.5 — —9.3 — — 6.6 — — LNU495 64697.2 0.5 0.07 12 — — — — — — LNU495 64697.30.6 0.11 28 10.9 0.13 22 7.6 0.06 10 LNU495 64698.2 0.5 0.15 16 — — — —— — LNU487 64702.1 — — — — — — 7.5 0.17 8 LNU487 64705.4 0.6 0.10 25 — —— — — — LNU487 64706.2 — — — 12.9 0.07 44 7.4 0.12 6 LNU474 64379.1 — —— — — — 7.6 0.11 9 LNU474 64382.2 — — — — — — 7.4 0.21 7 LNU474 64382.30.5 0.08 16 — — — 7.5 0.07 8 LNU474 64383.2 0.5 0.04 20 — — — 7.3 0.27 5LNU465 64020.1 0.5 0.08 17 — — — — — — LNU446 64546.2 — — — — — — 7.20.20 4 LNU446 64548.2 — — — — — — 7.2 0.27 3 LNU446 64549.3 0.5 0.04 15— — — — — — LNU443 64023.2 0.6 0.02 20 10.5 0.16 18 7.5 0.17 9 LNU44364024.4 — — — — — — 7.3 0.19 5 LNU443 64024.7 — — — — — — 7.8 0.01 13LNU436 64240.2 0.6 0.14 20 11.1 0.11 24 7.5 0.07 8 LNU436 64241.3 0.70.02 49 11.5 0.26 28 7.3 0.16 5 LNU436 64243.1 0.6 0.04 23 — — — — — —LNU379 64170.2 — — — — — — 7.7 0.09 11 LNU315 64224.1 0.6 0.24 22 — — —— — — LNU315 64225.2 0.6 0.06 40 — — — — — — LNU315 64226.3 0.6 0.09 3611.5 0.25 29 — — — LNU315 64227.3 0.6 0.04 34 — — — — — — CONT. — 0.5 —— 8.9 — — 6.9 — — LNU520 64156.13 — — — 10.9 0.19 11 7.9 0.16 7 LNU52064156.7 — — — 11.3 0.11 16 — — — LNU518 64015.4 — — — — — — 7.9 0.10 7LNU518 64016.3 — — — — — — 7.8 0.01 6 LNU502 64038.2 — — — — — — 7.60.23 4 LNU502 64039.3 — — — 10.9 0.26 11 7.8 0.08 6 LNU482 64164.8 — — —12.3 0.08 26 — — — LNU405 64158.9 0.9 0.04 43 14.9 0.04 52 8.0 0.25 8LNU405 64159.8 — — — — — — 7.9 0.20 8 LNU403 64239.1 0.8 0.07 35 12.20.18 24 — — — LNU393 63977.6 — — — 12.3 0.12 25 — — — LNU385 64245.3 — —— 11.8 0.03 20 7.7 0.13 4 LNU374 63997.2 — — — — — — 7.9 0.10 7 CONT. —0.6 — — 9.8 — — 7.4 — — LNU519 64678.1 0.5 0.02 32 10.3 0.12 29 — — —LNU519 64679.1 0.5 0.11 18 — — — — — — LNU519 64680.2 — — — 9.9 0.16 257.5 0.06 9 LNU519 64681.3 — — — 10.5 0.14 32 7.4 0.20 7 LNU519 64681.80.4 0.10 17 10.2 0.05 29 7.7 0.02 12 LNU500 64220.1 0.5 0.09 18 — — — —— — LNU500 64221.2 — — — 10.3 0.08 30 7.8 0.14 13 LNU500 64221.6 0.40.23 11 — — — — — — LNU500 64223.1 0.6 0.02 53 11.0 0.05 39 7.8 0.04 13LNU500 64223.2 0.4 0.18 15 9.9 0.07 25 7.9 L 15 LNU459 64541.4 — — — — —— 7.5 0.08 9 LNU459 64542.1 — — — — — — 7.5 0.07 9 LNU459 64542.3 0.50.01 36 10.4 0.07 32 — — — LNU459 64542.4 — — — 9.6 0.28 21 7.3 0.24 6LNU459 64543.2 — — — 9.4 0.26 19 7.6 0.06 10 LNU348 64472.2 0.5 0.01 2910.3 0.05 29 7.5 0.08 8 LNU348 64472.3 0.5 0.16 20 — — — — — — LNU34864474.1 0.5 L 29 — — — — — — LNU348 64474.2 0.5 0.13 30 12.2 0.09 54 7.40.13 8 LNU329 63427.3 0.4 0.23 12 10.0 0.05 26 — — — LNU329 63429.1 0.5L 35 12.6 L 59 8.4 L 22 LNU329 63430.3 0.4 0.27 11 — — — — — — LNU31664068.1 0.4 0.15 14 — — — — — — LNU316 64564.5 — — — — — — 7.7 0.02 12CONT. — 0.4 — — 7.9 — — 6.9 — — LNU437_H2 66104.1 0.6 L 36 10.8 0.02 436.9 0.07 12 LNU312 64002.3 0.5 0.15 16 — — — — — — LNU312 64002.5 0.50.18 18 9.8 0.07 30 6.7 0.12 9 CONT. — 0.4 — — 7.6 — — 6.1 — — LNU34763513.3 0.8 0.05 21 12.5 0.12 17 — — — CONT. — 0.7 — — 10.7 — — — — —LNU497 64206.2 — — — — — — 7.9 0.10 7 LNU497 64207.2 0.7 0.04 25 — — — —— — LNU497 64207.3 — — — 15.4 L 37 7.8 0.13 5 LNU491 64404.3 0.7 0.02 2514.9 L 32 7.9 0.15 7 LNU491 64404.6 0.7 0.04 28 14.5 0.03 29 8.1 0.04 10LNU491 64406.4 — — — — — — 7.8 0.17 5 LNU449 63892.1 0.7 0.05 22 — — — —— — LNU432 64066.2 0.7 0.14 28 — — — — — — CONT. — 0.5 — — 11.2 — — 7.4— — LNU510 64152.1 — — — — — — 7.7 0.03 8 LNU489 64012.1 — — — 12.1 0.1814 8.0 0.02 12 LNU438 63994.3 — — — — — — 7.5 0.14 5 LNU438 63994.5 — —— 13.1 0.02 22 7.7 0.05 7 LNU383 63982.1 — — — — — — 7.9 0.03 11 LNU38363982.7 — — — — — — 7.8 0.02 10 LNU354 63970.6 — — — — — — 7.7 0.05 8LNU354 63970.7 0.7 0.16 18 — — — — — — LNU354 63972.8 — — — — — — 7.90.06 11 LNU310 63904.1 — — — — — — 7.9 0.11 11 LNU310 63904.3 — — — 13.00.30 22 8.1 0.02 13 LNU299 64326.2 — — — 12.3 0.28 16 7.7 0.05 8 LNU29964328.2 0.7 0.26 11 — — — 7.5 0.16 5 LNU295 63899.5 — — — 12.9 0.10 217.9 0.02 11 LNU295 63899.8 — — — — — — 8.0 0.03 12 LNU295 63901.3 — — —— — — 7.5 0.14 5 LNU295 63902.3 — — — — — — 7.9 L 11 CONT. — 0.6 — —10.7 — — 7.1 — — LNU483 64803.2 0.6 0.08 19 13.4 L 42 — — — LNU48364803.3 0.6 0.02 39 14.8 0.06 58 — — — LNU483 64805.1 — — — 13.0 0.02 39— — — LNU483 64805.2 — — — 15.0 0.29 60 — — — LNU483 64806.2 — — — 11.70.15 25 — — — LNU378 64494.2 — — — 10.9 0.25 17 — — — LNU378 64495.4 0.50.19 16 — — — — — — LNU346 65008.2 0.6 0.23 19 — — — — — — LNU34665008.3 0.5 0.30 12 — — — — — — LNU290 64369.6 0.5 0.27 11 — — — — — —CONT. — 0.5 — — 9.4 — — — — — LNU510 64152.1 0.5 0.25 18 9.8 0.25 11 — —— LNU510 64154.2 0.5 0.27 20 11.0 0.07 25 7.7 0.09 8 LNU489 64010.8 — —— 10.3 0.19 17 — — — LNU489 64012.1 — — — 10.1 0.27 15 8.3 L 18 LNU48964013.1 — — — — — — 7.5 0.28 6 LNU438 63994.1 — — — — — — 7.5 0.29 6LNU438 63994.12 — — — 10.4 0.07 18 8.0 0.02 13 LNU438 63994.3 — — — — —— 7.5 0.16 7 LNU427 64178.6 — — — — — — 7.5 0.22 6 LNU383 63982.7 — — —— — — 7.7 0.12 9 LNU383 63983.1 — — — — — — 7.7 0.17 8 LNU354 63971.5 —— — — — — 7.5 0.28 6 LNU354 63972.8 — — — 9.9 0.20 12 7.7 0.07 9 LNU31063905.1 0.5 0.17 16 12.4 0.05 41 7.9 0.03 12 LNU295 63899.5 — — — 11.10.05 26 7.9 0.03 11 LNU295 63899.8 0.6 L 35 12.3 0.04 40 8.0 0.05 13LNU295 63901.3 — — — — — — 7.9 0.07 12 LNU295 63902.3 — — — — — — 7.60.17 8 CONT. — 0.4 — — 8.8 — — 7.1 — — LNU490 66092.3 0.4 0.24 19 — — —— — — LNU490 66093.1 — — — 7.0 0.22 18 6.6 0.25 4 LNU490 66093.2 — — —6.8 0.26 15 6.7 0.10 6 LNU490 66096.1 — — — 8.2 0.03 38 7.2 L 14 LNU44364023.2 — — — 8.2 0.08 37 — — — LNU443 64024.4 0.5 0.07 35 8.4 0.08 416.8 0.11 9 LNU443 64024.7 0.5 0.01 49 8.1 0.10 37 6.6 0.30 6 LNU43964615.4 — — — — — — 6.6 0.24 6 LNU439 64616.2 0.4 0.12 29 8.0 0.12 346.9 0.06 9 LNU439 64618.3 0.6 L 61 9.5 0.01 60 6.9 0.03 9 LNU437_H266104.1 0.5 0.09 30 8.7 L 46 6.8 0.13 9 LNU437_H2 66104.2 — — — 7.5 0.1627 6.7 0.13 6 LNU437_H2 66104.3 0.4 0.27 17 7.6 0.03 27 6.7 0.15 6LNU436 64240.1 — — — 8.1 0.02 36 7.0 0.01 11 LNU436 64240.2 0.4 0.09 298.4 0.03 41 6.9 0.05 9 LNU436 64241.3 — — — 8.2 0.01 37 6.8 0.05 8LNU436 64242.2 0.5 0.09 30 — — — — — — LNU436 64243.1 0.4 0.17 28 — — —— — — LNU433 64815.1 0.5 0.04 50 — — — — — — LNU433 64816.1 — — — 10.10.07 70 — — — LNU311 66099.1 — — — 8.4 0.15 42 — — — LNU311 66099.2 0.40.22 20 7.0 0.13 18 6.8 0.12 9 LNU298 66086.4 0.4 0.24 19 8.8 L 48 7.00.01 12 LNU298 66088.3 — — — 8.8 0.02 48 6.7 0.13 7 LNU298 66089.1 0.40.14 26 8.0 0.01 34 6.7 0.12 6 LNU298 66089.3 — — — 8.0 0.19 34 6.8 0.198 LNU293 65048.1 — — — 7.2 0.20 22 — — — LNU293 65049.1 — — — 8.5 L 44 —— — LNU293 65050.3 0.5 0.07 34 8.8 0.06 48 — — — LNU293 65051.3 0.5 0.1040 8.9 0.09 49 6.6 0.29 4 CONT. — 0.3 — — 5.9 — — 6.3 — — Table 70:CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant.

TABLE 71 Genes showing improved plant performance at nitrogen deficientconditions (T1 generation) Dry Weight [mg] Fresh Weight [mg] Gene NameAve. P-Val. % Incr. Ave. P-Val. % Incr. LNU488 54.5 0.06 51 115.3 0.0639 LNU466 50.5 0.13 40 107.7 0.14 30 LNU453 57.0 0.02 58 113.2 0.10 36LNU359 52.2 0.22 45 110.4 0.08 33 LNU358 — — — 108.4 0.08 30 LNU341 — —— 107.6 0.11 29 LNU309_H3 — — — 138.0 L 66 CONT. 36.0 — — 83.2 — — Table71: “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment.“p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant.

TABLE 72 Genes showing improved plant performance at nitrogen deficientconditions (T1 generation) Leaf Area [cm²] Roots Coverage [cm²] RootsLength [cm²] P- % P- % P- % Gene Name Ave. Val. Incr. Ave. Val. Incr.Ave. Val. Incr. LNU490 — — — 6.6 0.15 83 5.3 0.03 27 LNU417_H4 — — — — —— 4.8 0.08 14 LNU394 — — — 4.9 0.12 36 4.8 0.03 16 CONT. — — — 3.6 — —4.2 — — LNU488 0.6 0.02 42 8.7 0.03 81 6.5 L 28 LNU466 — — — 6.7 0.06 386.2 0.03 22 LNU453 0.7 0.04 43 6.5 0.20 35 — — — LNU359 0.6 0.25 29 7.20.10 50 6.3 0.10 25 LNU358 0.6 0.06 22 7.8 L 62 6.3 L 24 LNU341 0.6 0.0126 5.4 0.28 11 — — — LNU309_H3 0.7 L 55 9.3 L 93 7.0 L 37 CONT. 0.5 — —4.8 — — 5.1 — — Table 72: “CONT.”—Control; “Ave.”—Average; “% Incr.” = %increment; “p-val.”—p-value; L means that p-value is less than 0.01, p <0.1 was considered as significant.

The genes listed in Tables 73-74 have improved plant relative growthrate (relative growth rate of the leaf area, root coverage and rootlength) when grown under limiting nitrogen growth conditions, comparedto control plants (T2 and T1 generations). Plants showing fast growthrate show a better plant establishment in soil under nitrogen deficientconditions. Faster growth was observed when growth rate of leaf area androot length and coverage was measured.

TABLE 73 Genes showing improved plant growth rate at nitrogen deficientconditions (T2 generation) RGR Of RGR Of Root RGR Of Roots Leaf AreaCoverage Length P- % P- % P- % Gene Name Event # Ave. Val. Incr. Ave.Val. Incr. Ave. Val. Incr. LNU437_H2 66104.1 0.0 0.13 31 — — — — — —LNU437_H2 66104.2 0.0 0.09 41 — — — — — — LNU437_H2 66104.3 0.1 0.05 44— — — — — — LNU437_H2 66105.3 0.1 0.05 44 — — — — — — LNU426 66147.3 0.1L 73 — — — — — — LNU420 64006.3 0.0 0.29 26 — — — — — — LNU420 64007.30.0 0.16 29 — — — — — — LNU352 64199.1 0.1 L 92 — — — 0.8 0.08 13 LNU35264200.1 0.0 0.07 41 — — — — — — LNU352 64200.4 0.0 0.10 37 — — — 0.80.07 13 LNU292 64085.4 0.0 0.29 22 — — — 0.7 0.18  9 CONT. — 0.0 — — — —— 0.7 — — LNU483 64805.2 — — — — — — 0.7 0.17 11 LNU464 65076.1 — — — —— — 0.7 0.24  9 LNU439 64616.2 0.1 0.25 15 — — — — — — LNU439 64616.30.1 0.18 15 — — — — — — LNU439 64618.3 0.1 0.25 13 — — — — — — LNU42563911.9 0.1 0.05 21 — — — — — — LNU414 64479.1 0.1 0.03 25 — — — — — —LNU414 64480.2 0.1 0.06 26 — — — — — — LNU346 65008.2 0.1 0.09 20 — — —— — — LNU336 64448.2 0.1 L 40 — — — — — — CONT. — 0.1 — — — — — 0.6 — —LNU473 65770.4 0.1 0.28 14 — — — 0.8 0.02 14 LNU470 64228.3 — — — — — —0.7 0.16  9 LNU470 64229.1 0.1 0.14 18 — — — 0.8 0.08 11 LNU460 64359.3— — — — — — 0.8 0.02 15 LNU421 64303.3 0.1 0.08 24 — — — — — — LNU40864248.10 0.1 0.17 16 — — — — — — LNU380 65764.1 — — — — — — 0.7 0.13  8LNU380 65764.2 — — — — — — 0.7 0.24  5 LNU380 65764.3 — — — — — — 0.80.03 11 LNU380 65765.4 — — — — — — 0.8 0.05 10 LNU340 64290.7 0.1 0.1021 — — — 0.8 0.05 12 LNU340 64292.5 — — — — — — 0.7 0.16  6 LNU33164214.2 0.1 0.01 31 — — — 0.7 0.07 10 LNU331 64215.1 0.1 L 44 — — — 0.80.02 14 CONT. — 0.1 — — — — — 0.7 — — LNU430 63934.3 0.0 0.20 18 — — — —— — LNU407 64219.2 0.1 0.18 22 — — — — — — LNU402 63914.2 0.0 0.29 16 —— — — — — LNU335 64168.15 — — — — — — 0.7 0.30 10 LNU335 64169.2 0.00.17 20 — — — — — — LNU301 63927.3 0.1 0.16 29 — — — — — — LNU30163950.3 0.1 L 64 — — — — — — CONT. — 0.0 — — — — — 0.7 — — LNU45063708.3 0.1 0.02 29 — — — — — — LNU450 63710.2 0.1 0.07 21 — — — — — —LNU450 63712.3 0.1 0.04 28 — — — — — — LNU416 64136.4 0.1 0.09 21 — — —0.8 0.20 11 LNU412 63940.12 0.1 0.20 22 — — — — — — LNU412 63940.8 0.10.25 16 — — — — — — LNU359 66154.6 0.1 0.13 16 — — — — — — LNU29365051.3 0.1 0.19 18 — — — — — — CONT. — 0.1 — — — — — 0.7 — — LNU49864186.2 — — — — — — 0.7 0.23  8 LNU498 64186.3 — — — — — — 0.7 0.14  9LNU493 64191.2 0.0 0.14 17 — — — — — — LNU455 64187.5 0.0 0.13 16 — — —— — — LNU328 64150.4 — — — — — — 0.7 0.07 11 LNU328 64151.1 0.0 0.21 15— — — — — — LNU322 63917.2 — — — — — — 0.7 0.30  7 LNU322 63918.1 0.00.16 14 — — — — — — LNU317 64097.2 — — — — — — 0.7 0.11 11 LNU30564114.1 — — — — — — 0.7 0.28  6 CONT. — 0.0 — — — — — 0.7 — — LNU49564697.3 — — — — — — 0.7 0.04 14 LNU495 64698.2 — — — — — — 0.7 0.12 11LNU487 64702.3 — — — — — — 0.7 0.07 17 LNU487 64705.4 — — — — — — 0.70.20  8 LNU487 64706.2 0.1 0.11 16 — — — — — — LNU474 64381.1 — — — — —— 0.7 0.17 10 LNU465 64020.1 0.1 0.14 17 — — — 0.7 0.11 14 LNU46564020.4 0.1 0.08 22 — — — 0.7 0.14 10 LNU446 64546.2 0.1 0.03 24 — — — —— — LNU446 64549.2 — — — — — — 0.7 0.10 11 LNU443 64023.2 0.1 0.06 24 —— — 0.7 0.16 11 LNU443 64023.9 — — — — — — 0.7 0.04 15 LNU443 64024.7 —— — — — — 0.7 0.07 13 LNU436 64240.1 0.1 0.13 20 — — — — — — LNU43664240.2 — — — — — — 0.7 0.26  9 LNU436 64241.3 0.1 0.14 17 — — — — — —LNU436 64243.1 0.1 0.01 24 — — — 0.7 0.07 13 LNU379 64170.2 — — — — — —0.7 0.23 10 LNU379 64170.3 — — — — — — 0.7 0.20  8 LNU379 64170.4 — — —— — — 0.7 0.25  9 LNU379 64172.2 — — — — — — 0.7 0.18  9 LNU315 64224.30.1 0.18 14 — — — 0.8 0.01 23 LNU315 64225.1 0.1 0.21 13 — — — — — —CONT. — 0.0 — — — — — 0.6 — — LNU497 64207.3 — — — — — — 0.6 0.28  7LNU491 64405.2 0.1 0.04 38 — — — 0.7 0.06 12 LNU491 64406.4 0.1 0.04 37— — — 0.7 L 24 LNU449 64571.3 0.1 0.06 32 — — — 0.7 0.01 19 LNU43564464.3 0.1 L 67 — — — 0.7 0.04 12 LNU435 64465.1 — — — — — — 0.7 0.2910 LNU432 64065.2 — — — — — — 0.7 0.03 11 LNU432 64559.2 0.1 0.05 31 — —— 0.7 L 19 LNU432 64560.3 0.1 0.22 19 — — — — — — LNU432 64560.5 0.10.13 24 — — — 0.7 L 17 LNU367 64398.2 0.1 0.18 25 — — — 0.7 0.15 10LNU367 64399.1 — — — — — — 0.7 0.26  8 LNU364 64441.2 0.1 0.21 22 — — —0.7 0.20 12 LNU362 64324.3 0.1 0.27 19 — — — — — — CONT. — 0.1 — — — — —0.6 — — LNU495 64697.2 0.1 0.01 21 — — — — — — LNU495 64697.3 0.1 L 42 —— — 0.7 0.01 25 LNU495 64698.2 0.1 0.01 31 — — — — — — LNU495 64701.30.1 0.10 23 — — — 0.7 0.06 15 LNU487 64702.1 — — — — — — 0.7 0.05 17LNU487 64702.3 — — — — — — 0.6 0.26 10 LNU487 64704.2 — — — — — — 0.60.23 11 LNU487 64705.4 0.1 L 38 — — — — — — LNU487 64706.2 0.1 0.04 22 —— — — — — LNU474 64379.1 — — — — — — 0.7 0.18 13 LNU474 64382.3 0.1 0.0226 — — — — — — LNU474 64383.2 0.1 L 29 — — — 0.7 0.18 13 LNU465 64020.10.1 0.10 19 — — — — — — LNU465 64021.7 0.0 0.30 11 — — — — — — LNU44664546.3 0.1 0.15 21 — — — — — — LNU446 64549.3 0.1 0.04 21 — — — — — —LNU443 64023.2 0.1 L 31 — — — 0.7 0.27 12 LNU443 64024.7 — — — — — — 0.7L 23 LNU436 64240.1 0.0 0.15 13 — — — — — — LNU436 64240.2 0.1 0.04 29 —— — 0.7 0.13 15 LNU436 64241.3 0.1 L 55 — — — — — — LNU436 64242.2 0.10.14 17 — — — — — — LNU436 64243.1 0.1 0.01 31 — — — — — — LNU37964170.2 — — — — — — 0.7 0.07 19 LNU379 64172.1 0.0 0.20 15 — — — 0.60.24 10 LNU315 64224.1 0.1 0.13 25 — — — — — — LNU315 64225.2 0.1 0.0145 — — — — — — LNU315 64226.3 0.1 0.01 45 — — — — — — LNU315 64227.3 0.1L 36 — — — — — — CONT. — 0.0 — — — — — 0.6 — — LNU518 64015.4 — — — — —— 0.7 0.21 10 LNU502 64039.3 — — — — — — 0.7 0.25  9 LNU482 64164.1 — —— — — — 0.7 0.12 12 LNU405 64158.9 0.1 0.06 42 — — — 0.7 0.28 13 LNU40364237.6 — — — — — — 0.7 0.21  9 LNU403 64239.1 0.1 0.13 33 — — — — — —LNU374 63997.2 — — — — — — 0.7 0.18 12 CONT. — 0.1 — — — — — 0.6 — —LNU519 64678.1 0.1 0.03 33 — — — — — — LNU519 64679.1 0.0 0.29 15 — — —— — — LNU519 64680.2 — — — — — — 0.7 0.27 10 LNU519 64681.3 — — — — — —0.7 0.29 10 LNU519 64681.8 0.0 0.18 18 — — — 0.7 0.27 11 LNU500 64220.10.0 0.22 17 — — — — — — LNU500 64223.1 0.1 L 59 — — — 0.8 0.11 15 LNU50064223.2 0.0 0.24 17 — — — 0.8 0.04 18 LNU459 64542.1 — — — — — — 0.80.16 12 LNU459 64542.3 0.1 0.01 38 — — — — — — LNU459 64542.4 — — — — —— 0.7 0.21 11 LNU459 64543.2 — — — — — — 0.8 0.14 13 LNU348 64472.2 0.10.03 31 — — — — — — LNU348 64472.3 0.0 0.13 23 — — — — — — LNU34864474.1 0.1 0.04 31 — — — — — — LNU348 64474.2 0.1 0.06 33 — — — — — —LNU329 63429.1 0.1 0.01 35 — — — 0.8 0.05 19 LNU316 64068.1 0.0 0.24 16— — — — — — CONT. — 0.0 — — — — — 0.7 — — LNU499 64146.11 — — — — — —0.6 0.24 11 LNU490 66093.2 — — — — — — 0.6 0.07 18 LNU490 66095.2 — — —— — — 0.6 0.17 14 LNU437_H2 66104.1 0.1 0.04 38 — — — 0.6 0.11 17LNU437_H2 66104.2 — — — — — — 0.6 0.17 14 LNU395 64143.6 — — — — — — 0.60.07 17 LNU312 64002.5 — — — — — — 0.7 0.04 21 CONT. — 0.0 — — — — — 0.5— — LNU392 63696.1 — — — — — — 0.7 0.23 11 LNU347 63510.4 — — — — — —0.7 0.22 11 LNU347 63513.3 0.1 0.01 25 — — — — — — CONT. — 0.1 — — — — —0.6 — — LNU497 64207.2 0.1 0.16 19 — — — — — — LNU491 64404.3 0.1 0.0821 — — — — — — LNU491 64404.6 0.1 0.09 23 — — — — — — LNU449 63892.1 0.10.24 15 — — — — — — LNU432 64066.2 0.1 0.19 20 — — — — — — CONT. — 0.1 —— — — — — — — LNU489 64012.1 — — — — — — 0.8 0.29  9 LNU354 63970.6 — —— — — — 0.8 0.17 11 LNU354 63972.8 — — — — — — 0.8 0.25 10 LNU29964328.2 0.1 0.30 13 — — — — — — LNU295 63901.3 0.1 0.15 19 — — — 0.80.26  9 CONT. — 0.1 — — — — — 0.7 — — LNU483 64803.3 0.1 0.09 34 — — — —— — CONT. — 0.0 — — — — — — — — LNU510 64154.2 0.1 0.12 30 — — — 0.70.24 10 LNU489 64012.1 — — — — — — 0.8 0.04 18 LNU438 63994.1 — — — — —— 0.7 0.26  9 LNU438 63994.12 — — — — — — 0.8 0.05 16 LNU438 63994.3 — —— — — — 0.7 0.18 11 LNU438 63994.5 — — — — — — 0.7 0.20 11 LNU42764178.6 — — — — — — 0.7 0.24  9 LNU427 64180.3 — — — — — — 0.7 0.27 11LNU383 63982.7 — — — — — — 0.7 0.24 10 LNU383 63983.1 — — — — — — 0.70.23 10 LNU354 63972.8 — — — — — — 0.7 0.29  8 LNU310 63905.1 0.1 0.1329 — — — 0.8 0.09 16 LNU310 63906.2 — — — — — — 0.7 0.18 12 LNU29563899.8 0.1 0.03 39 — — — 0.8 0.04 18 LNU295 63901.3 — — — — — — 0.80.04 17 LNU295 63902.3 — — — — — — 0.7 0.25 10 CONT. — 0.0 — — — — — 0.7— — LNU490 66092.3 0.0 0.29 25 — — — — — — LNU490 66093.1 — — — — — —0.6 0.12 10 LNU443 64023.2 — — — — — — 0.6 0.23  9 LNU443 64024.4 0.00.15 36 — — — 0.6 0.25  9 LNU443 64024.7 0.0 0.13 42 — — — — — — LNU43964614.4 — — — — — — 0.6 0.24  9 LNU439 64616.2 0.0 0.12 38 — — — 0.60.16 10 LNU439 64618.3 0.1 0.01 66 — — — — — — LNU437_H2 66104.1 0.00.23 28 — — — — — — LNU437_H2 66104.2 — — — — — — 0.6 0.06 13 LNU437_H266104.3 — — — — — — 0.6 0.11 11 LNU436 64240.1 — — — — — — 0.6 0.06 14LNU436 64240.2 0.0 0.20 31 — — — — — — LNU436 64241.3 — — — — — — 0.60.24  9 LNU436 64242.2 0.0 0.23 29 — — — — — — LNU436 64243.1 0.0 0.2429 — — — — — — LNU433 64815.1 0.1 0.04 54 — — — — — — LNU311 66099.1 — —— — — — 0.6 0.28 12 LNU298 66086.4 — — — — — — 0.6 0.08 13 LNU29866088.3 — — — — — — 0.6 0.03 16 LNU298 66089.1 0.0 0.16 34 — — — 0.60.09 13 LNU298 66089.3 — — — — — — 0.6 0.17 11 LNU293 65048.1 — — — — —— 0.6 0.09 14 LNU293 65049.1 — — — — — — 0.6 0.14 12 LNU293 65050.3 0.00.22 31 — — — — — — LNU293 65051.3 0.0 0.10 44 — — — 0.6 0.05 14 CONT. —0.0 — — — — — 0.5 — — Table 73: “CONT.”—Control; “Ave.”—Average; “%Incr.” = % increment; “p-val.”—p-value; L means that p-value is lessthan 0.01, p < 0.1 was considered as significant.

TABLE 74 Genes showing improved plant growth rate at nitrogen deficientconditions (T1 generation) RGR Of RGR Of Root RGR Of Leaf Area CoverageRoots Length P- % P- % P- % Gene Name Ave. Val. Incr. Ave. Val. Incr.Ave. Val. Incr. LNU490 0.1 0.29 18 — — — 0.6 L 27 LNU417_H4 — — — — — —0.5 0.14 14 LNU394 — — — — — — 0.6 0.05 17 CONT. 0.1 — — — — — 0.5 — —LNU488 0.1 L 55 — — — 0.7 L 34 LNU466 0.1 0.16 17 — — — 0.7 L 28 LNU4530.1 L 49 — — — 0.6 0.20 10 LNU359 0.1 0.05 34 — — — 0.7 L 29 LNU358 0.10.02 28 — — — 0.7 L 28 LNU341 0.1 0.03 27 — — — — — — LNU309_H3 0.1 L 67— — — 0.8 L 42 CONT. 0.0 — — — — — 0.5 — — Table 74: “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant.

The genes listed in Tables 75-78 improved plant NUE when grown atstandard nitrogen concentration levels. These genes produced largerplant biomass (plant fresh and dry weight; leaf area, root coverage androots length) when grown under standard nitrogen growth conditions,compared to control plants in T2 (Tables 75-76) and T1 (Tables 77-78)generations. Larger plant biomass under this growth conditions indicatesthe high ability of the plant to better metabolize the nitrogen presentin the medium. Plants producing larger root biomass have betterpossibilities to absorb larger amount of nitrogen from soil.

TABLE 75 Genes showing improved plant performance at standard nitrogengrowth conditions (T2 generation) Gene Dry Weight [mg] Fresh Weight [mg]Name Event # Ave. P-Val. % Incr. Ave. P-Val. % Incr. LNU437_H2 66104.14.2 0.09 38 — — — LNU437_H2 66104.2 5.2 L 70  78.6 L 48 LNU437_H266104.3 5.5 L 80  91.3 0.03 71 LNU437_H2 66105.1 3.7 0.12 21 — — —LNU426 66146.1 4.4 0.11 46  71.1 0.06 34 LNU426 66147.3 6.7 L 122  117.6L 121  LNU420 64007.3 4.6 0.08 52  68.5 0.22 29 LNU420 64008.4 5.2 L 72 77.3 L 45 LNU420 64009.1 4.2 0.23 40 — — — LNU359 66154.6 3.7 0.19 21 —— — LNU359 66156.1 5.5 L 82  68.6 0.02 29 LNU352 64199.1 7.7 L 154 124.9 L 135  LNU352 64200.1 3.9 0.21 30 — — — LNU352 64200.10 4.0 0.0232  67.3 0.05 26 LNU352 64200.4 4.0 0.23 32 — — — LNU352 64201.1 4.00.18 34 — — — LNU292 64085.4 4.6 L 52  72.6 L 36 CONT. — 3.0 — —  53.2 —— LNU483 64803.2 7.1 0.07 24 122.8 0.06 23 LNU483 64803.3 — — — 120.70.17 21 LNU483 64805.1 6.9 0.28 20 116.9 0.05 18 LNU483 64805.2 7.9 0.0637 132.6 0.02 33 LNU477 63886.1 7.4 0.09 29 130.0 0.06 31 LNU477 63888.1— — — 124.5 0.15 25 LNU477 63889.5 — — — 108.2 0.25  9 LNU447 65000.17.5 0.27 31 141.4 0.09 42 LNU447 65000.4 — — — 116.6 0.24 17 LNU44765002.3 7.4 0.19 29 140.6 0.05 41 LNU447 65004.1 — — — 131.6 0.15 32LNU439 64616.2 7.9 0.18 37 132.8 0.01 34 LNU439 64616.3 8.9 0.02 56154.7 0.02 56 LNU425 63910.9 — — — 116.5 0.19 17 LNU425 63911.11 — — —118.7 0.03 19 LNU425 63911.12 — — — 116.9 0.12 18 LNU425 63911.9 7.50.15 30 134.3 0.10 35 LNU414 64475.1 8.3 0.14 44 143.4 0.16 44 LNU41464480.2 — — — 120.2 0.17 21 LNU336 64447.2 9.0 0.13 56 172.0 0.02 73LNU336 64448.3 — — — 131.3 0.10 32 LNU336 64449.3 6.7 0.29 17 113.6 0.0914 LNU336 64449.4 7.8 0.11 35 136.5 0.03 37 CONT. — 5.8 — —  99.4 — —LNU473 65770.4 — — — 110.9 0.20  8 LNU470 64228.3 6.5 0.22 24 126.1 0.2223 LNU470 64229.1 5.9 0.19 13 — — — LNU460 64359.3 9.7 0.05 86 173.90.03 69 LNU460 64362.1 6.0 0.25 15 — — — LNU408 64248.10 6.2 0.14 18115.2 0.10 12 LNU408 64250.8 6.7 0.02 29 135.8 0.01 32 LNU380 65765.46.2 0.13 20 123.1 0.20 20 LNU331 64215.1 6.9 0.25 32 116.8 0.16 14LNU306 64131.2 6.0 0.22 14 119.4 0.04 16 CONT. — 5.2 — — 102.8 — —LNU412 63940.8 — — — 118.0 0.24 41 LNU407 64218.1 — — — 103.4 0.19 24LNU407 64219.2 — — — 106.8 0.10 28 LNU402 63915.1 — — — 105.5 0.27 26LNU384 64161.7 — — — 121.1 0.22 45 LNU360 64029.3 — — — 109.0 0.29 30LNU335 64168.18 — — — 101.5 0.26 21 LNU301 63927.5 — — — 122.8 0.12 47CONT. — — — —  83.6 — — LNU450 63708.3 — — — 109.0 0.14 22 LNU45063710.2 — — —  99.8 0.24 11 LNU426 66148.1 7.0 0.22 24 — — — LNU41664134.11 — — — 113.9 0.05 27 LNU416 64134.2 — — — 108.3 0.28 21 LNU41664136.4 7.0 0.04 24 111.6 0.10 25 LNU412 63940.8 7.6 0.27 35 125.8 0.0740 LNU349 63990.2 6.9 0.07 21 — — — LNU349 63990.4 8.0 0.01 42 129.0 L44 LNU293 65048.1 — — — 116.2 0.17 30 LNU293 65051.3 6.4 0.15 13 120.60.01 35 CONT. — 5.6 — —  89.6 — — LNU498 64185.3 5.0 L 64 102.5 L 46LNU493 64190.1 4.1 0.04 35  83.0 0.24 18 LNU493 64191.4 4.9 0.09 61 — —— LNU455 64187.5 4.6 0.09 52 111.3 L 59 LNU455 64189.2 4.0 0.14 30 — — —LNU455 64189.4 5.2 0.04 69 112.1 0.10 60 LNU343 64208.1 4.5 0.02 46126.1 0.01 80 LNU343 64209.1 4.0 0.18 29 107.1 0.16 53 LNU328 64150.14.2 0.06 37 — — — LNU328 64150.2 4.0 0.27 32  89.2 0.13 27 LNU32864150.4 3.7 0.20 20  87.4 0.13 25 LNU328 64151.1 — — —  88.2 0.21 26LNU328 64151.2 — — —  85.9 0.04 22 LNU322 63917.2 4.4 0.20 44 106.5 0.2552 LNU322 63918.1 4.1 0.28 34  99.4 0.12 42 LNU322 63918.3 4.3 0.03 42 93.2 0.04 33 LNU317 64097.3 3.6 0.22 19  83.6 0.21 19 LNU305 64111.1 —— —  77.8 0.30 11 LNU305 64111.3 4.7 0.01 53 111.1 0.15 58 LNU30564115.1 3.8 0.20 25  89.7 0.27 28 CONT. — 3.1 — —  70.1 — — LNU49564701.3 4.2 0.30  8 — — — LNU487 64702.1 — — —  78.4 0.12 25 LNU48764702.3 4.6 0.16 18  76.7 0.06 22 LNU474 64381.1 6.0 0.10 53 102.8 0.0764 LNU474 64382.2 5.2 L 31  79.6 0.05 27 LNU474 64383.2 — — —  74.2 0.2218 LNU465 64020.1 5.2 0.11 32  98.1 0.26 56 LNU465 64022.2 4.6 0.05 18 81.0 0.05 29 LNU446 64546.2 4.8 0.13 22  76.8 0.27 22 LNU443 64024.37.1 0.02 82 144.5 L 130 LNU443 64024.7 4.8 0.25 22 — — — LNU436 64240.16.2 0.16 59  91.7 0.04 46 LNU436 64240.2 4.8 0.16 21  85.4 0.03 36LNU436 64242.2 12.3  0.03 213  181.2 L 189  LNU436 64243.1 — — —  81.90.21 31 LNU379 64170.4 — — —  81.2 0.21 29 LNU315 64224.1 5.2 0.15 31 79.0 0.21 26 LNU315 64224.3 5.2 0.17 33  91.2 L 45 LNU315 64225.1 — — — 78.0 0.18 24 LNU315 64226.3 5.5 L 40 105.2 0.04 68 LNU315 64227.3 5.9 L51  88.3 0.06 41 CONT. — 3.9 — —  62.7 — — LNU497 64207.2 7.0 0.27 18 —— — LNU491 64406.4 8.1 0.27 37 165.8 0.30 38 LNU449 63890.1 6.9 0.10 17— — — LNU449 64571.3 9.4 0.11 60 — — — LNU432 64065.2 9.9 0.09 67 179.40.13 50 LNU432 64559.2 8.8 0.19 50 185.1 0.20 55 LNU432 64560.3 — — —144.1 0.22 20 LNU367 64398.2 9.1 0.16 55 196.8 0.14 64 LNU367 64399.17.8 0.03 32 174.7 L 46 CONT. — 5.9 — — 119.8 — — LNU436 64241.3 6.9 0.1047 — — — LNU379 64170.3 5.4 0.19 13 — — — LNU379 64172.2 7.1 0.14 50156.5 0.09 50 LNU315 64225.2 7.1 L 49 138.9 0.02 33 CONT. — 4.7 — —104.3 — — LNU519 64678.1 4.1 0.04 28  74.2 0.04 23 LNU519 64679.1 5.00.06 56  78.1 L 29 LNU519 64681.3 3.8 0.24 19 — — — LNU519 64681.8 3.90.04 21  74.0 L 23 LNU459 64542.3 4.2 0.12 29  75.4 0.17 25 LNU45964543.2 3.9 0.16 22  73.0 0.29 21 LNU348 64472.2 — — —  76.2 0.10 26LNU348 64472.3 — — —  65.7 0.16  9 LNU348 64474.1 4.0 0.09 24  74.1 0.0423 LNU348 64474.2 4.8 0.05 48  81.8 0.05 35 LNU329 63427.3 — — —  72.90.10 21 LNU329 63428.2 4.0 0.02 24  82.2 0.10 36 LNU329 63429.1 4.2 0.2332 — — — LNU329 63430.3 3.7 0.12 14  68.1 0.08 13 CONT. — 3.2 — —  60.4— — LNU499 64146.12 — — — 105.9 0.30 20 LNU312 64002.5 6.0 0.07 18 — — —LNU311 66100.3 — — — 100.9 0.23 14 CONT. — 5.1 — —  88.2 — — LNU39263697.4 — — — 152.5 0.13 24 LNU392 63701.2 8.0 0.22 21 150.6 0.24 22CONT. — 6.6 — — 123.3 — — LNU497 64207.2 6.0 0.03 49 103.5 0.02 40LNU497 64207.3 5.2 0.16 29 — — — LNU491 64404.3 6.3 0.02 57 108.3 0.0246 LNU491 64404.6 5.0 0.17 24  89.8 0.15 21 LNU449 63890.1 — — —  92.10.09 24 LNU449 64570.1 6.6 0.01 64 109.3 0.02 47 LNU432 64065.2 5.3 0.0632  94.4 0.16 27 LNU432 64066.2 6.1 0.05 53 115.4 L 56 LNU432 64559.25.0 0.22 24  90.2 0.18 22 LNU367 64398.2 5.6 0.01 41  93.0 0.09 25LNU367 64398.3 — — —  88.0 0.26 19 LNU362 64323.1 5.2 0.21 31 — — —CONT. — 4.0 — —  74.2 — — LNU438 63994.12 8.8 0.18 36 — — — LNU43863994.5 7.5 0.25 16 — — — LNU310 63904.3 10.4  0.10 62 178.4 0.29 33CONT. — 6.5 — — 134.4 — — LNU483 64803.2 5.7 L 42 115.9 L 35 LNU48364803.3 5.2 0.05 30 105.3 0.05 22 LNU483 64805.1 — — — 102.7 0.24 19LNU483 64805.2 5.1 0.23 27 109.1 0.04 27 LNU483 64806.2 5.6 0.02 40110.1 0.10 28 LNU435 64463.3 7.6 0.05 89 150.3 0.03 75 LNU378 64494.2 —— — 112.0 0.08 30 LNU346 65008.2 4.9 0.19 20 — — — CONT. — 4.0 — —  86.1— — LNU489 64010.8 — — —  90.2 0.25 28 LNU438 63994.5 6.4 0.13 68 106.30.07 51 LNU310 63904.1 6.0 0.14 59  93.5 0.23 33 LNU295 63899.8 6.0 0.1159 110.0 0.12 56 LNU295 63902.3 6.6 0.10 75 125.0 0.12 78 CONT. — 3.8 ——  70.3 — — LNU490 66093.2 4.2 0.28 18 — — — LNU443 64023.2 4.3 0.29 23— — — LNU443 64024.4 4.3 0.25 22 — — — LNU443 64024.7 5.6 0.01 60  97.70.03 35 LNU439 64614.4 5.6 0.15 61 109.5 0.21 51 LNU439 64618.3 6.0 0.2770 109.1 0.21 51 LNU436 64240.2 4.5 0.26 27  90.6 0.10 25 LNU436 64242.25.4 0.01 53 101.0 0.03 39 LNU433 64815.2 7.8 L 123  132.9 L 83 LNU43364816.1 5.0 0.18 42 — — — LNU293 65051.3 4.2 0.28 20 — — — CONT. — 3.5 ——  72.5 — — Table 75: “CONT.”—Control; “Ave.”—Average; “% Incr.” = %increment; “p-val.”—p-value; L means that p-value is less than 0.01, p <0.1 was considered as significant.

TABLE 76 Genes showing improved plant performance at standard nitrogengrowth conditions (T2 generation) Roots Coverage Leaf Area [cm²] [cm²]Roots Length [cm²] P- % P- % P- % Gene Name Event # Ave. Val. Incr. Ave.Val. Incr. Ave. Val. Incr. LNU437_H2 66104.1 — — — 5.1 0.21 27 6.8 0.0217 LNU437_H2 66104.2 0.4 0.01 35 5.1 0.04 28 — — — LNU437_H2 66104.3 0.5L 53 6.7 0.04 67 6.8 0.04 18 LNU437_H2 66105.1 0.4 0.20 14 — — — — — —LNU437_H2 66105.3 0.4 0.17 15 — — — — — — LNU426 66147.1 — — — — — — 6.40.04 11 LNU426 66147.3 0.6 L 74 5.8 L 46 6.3 0.17 10 LNU420 64006.3 — —— — — — 6.3 0.11  9 LNU420 64007.3 — — — 4.9 0.19 22 6.3 0.04 10 LNU42064008.4 0.4 0.07 24 5.1 0.02 28 6.3 0.19  9 LNU420 64009.1 — — — — — —6.1 0.21  7 LNU359 66154.6 0.4 0.26 14 — — — — — — LNU352 64199.1 0.6 L83 7.1 L 78 7.0 L 22 LNU352 64200.1 0.4 0.14 24 — — — — — — LNU35264200.10 0.4 0.14 20 6.0 0.08 49 6.6 0.06 14 LNU352 64200.4 — — — 4.70.15 19 6.3 0.15  9 LNU292 64084.1 — — — — — — 6.1 0.25  5 LNU29264085.4 0.4 0.25 12 4.7 0.10 17 6.6 0.02 14 CONT. — 0.3 — — 4.0 — — 5.8— — LNU483 64803.2 0.7 0.05 15 10.7  0.01 54 7.3 0.02 11 LNU483 64803.30.7 0.10 18 11.0  L 57 — — — LNU483 64805.1 — — — 13.7  L 97 7.5 L 15LNU483 64805.2 0.7 0.06 21 10.7  L 53 — — — LNU483 64806.2 — — — 8.60.29 23 — — — LNU477 63886.1 0.7 0.04 20 — — — — — — LNU477 63888.1 0.70.14 17 — — — 7.1 0.02  9 LNU477 63889.2 — — — — — — 6.9 0.17  6 LNU47763889.5 — — — — — — 6.8 0.12  5 LNU464 65076.1 — — — — — — 6.9 L  6LNU464 65076.4 0.7 0.15 17 — — — 6.7 0.30  2 LNU447 65000.1 0.7 0.08 209.2 0.07 32 7.1 0.04 10 LNU447 65000.4 0.6 0.26 12 — — — 7.0 0.08  8LNU447 65002.3 0.7 0.12 18 8.1 0.11 16 6.8 0.29  4 LNU439 64614.4 — — —— — — 6.9 0.29  6 LNU439 64616.2 0.7 L 29 — — — 6.9 0.04  6 LNU43964616.3 0.8 L 40 9.2 0.04 31 7.5 0.02 14 LNU439 64618.3 0.7 0.16 24 8.60.04 23 7.0 0.25  8 LNU425 63910.9 — — — 8.2 0.18 17 — — — LNU42563911.11 0.7 0.14 13 12.6  L 80 7.3 L 11 LNU425 63911.12 0.6 0.24  8 — —— 6.8 0.17  4 LNU425 63911.7 — — — — — — 6.7 0.22  3 LNU425 63911.9 0.70.11 19 8.5 0.24 21 7.0 0.13  7 LNU414 64475.1 0.8 0.11 30 — — — 6.90.01  6 LNU414 64480.2 0.7 0.25 18 8.8 0.25 26 7.0 L  8 LNU346 65006.1 —— — — — — 6.9 0.03  6 LNU346 65007.3 — — — — — — 6.8 0.04  4 LNU34665008.2 — — — 7.9 0.17 13 7.1 0.14  8 LNU346 65008.3 — — — — — — 6.80.05  5 LNU346 65009.2 — — — — — — 7.0 L  7 LNU336 64447.2 0.8 0.05 319.1 0.12 30 7.0 0.06  8 LNU336 64448.2 — — — 9.0 0.28 29 7.2 L 10 LNU33664448.3 0.7 0.12 19 — — — — — — LNU336 64449.4 0.7 0.10 14 — — — 6.80.28  5 CONT. — 0.6 — — 7.0 — — 6.5 — — LNU470 64228.3 0.7 0.10 15 10.6 L 39 7.5 0.03 12 LNU470 64229.1 — — — 9.0 0.09 19 7.7 0.08 14 LNU46064359.3 0.8 0.04 39 11.6  0.01 53 7.7 L 14 LNU460 64362.1 0.6 0.29  6 —— — 7.1 0.03  5 LNU421 64302.7 — — — 9.2 0.23 21 7.6 0.02 13 LNU40864248.10 0.6 0.30  8 10.2  0.05 34 7.7 L 15 LNU408 64248.12 — — — — — —7.3 0.21  9 LNU408 64248.16 — — — — — — 7.2 0.16  8 LNU408 64250.8 0.70.08 13 10.8  0.08 42 7.7 0.05 15 LNU380 65764.2 — — — — — — 7.1 0.19  5LNU380 65765.4 — — — 9.1 0.11 20 7.3 0.30  9 LNU340 64290.11 — — — — — —7.7 L 14 LNU340 64290.7 — — — 9.8 0.10 29 7.9 L 17 LNU340 64291.10 — — —— — — 7.8 L 16 LNU340 64292.5 — — — — — — 6.9 0.17  3 LNU331 64214.2 — —— — — — 7.2 0.14  7 LNU331 64215.1 — — — — — — 7.4 0.08 10 LNU33164215.3 — — — — — — 7.4 0.04 10 LNU306 64131.2 — — — 9.8 0.12 29 7.40.13 10 CONT. — 0.6 — — 7.6 — — 6.7 — — LNU456 63991.2 — — — 6.4 0.13 166.8 0.24  7 LNU456 63991.8 — — — 6.3 0.17 14 6.8 0.12  7 LNU412 63940.1— — — — — — 6.9 0.01  8 LNU412 63941.2 — — — — — — 7.0 0.03 10 LNU40764218.2 — — — — — — 7.2 L 13 LNU407 64219.1 — — — 6.4 0.22 16 7.2 L 13LNU407 64219.2 0.5 0.08 14 — — — — — — LNU402 63914.2 — — — — — — 6.70.19  5 LNU384 64161.3 — — — 7.3 0.16 32 7.2 0.07 12 LNU384 64161.7 — —— 6.9 0.12 24 7.0 0.16  9 LNU360 64029.2 — — — 6.7 L 22 6.7 0.21  5LNU360 64029.3 — — — 6.8 0.07 22 7.0 0.12 10 LNU360 64030.4 — — — 7.30.06 33 7.4 0.05 16 LNU360 64030.6 — — — 6.3 0.29 14 7.0 0.01 10 LNU33564168.15 — — — — — — 6.7 0.27  4 LNU301 63950.3 — — — 6.7 0.24 21 7.3 L14 CONT. — 0.5 — — 5.5 — — 6.4 — — LNU450 63708.3 0.7 0.19 16 7.7 0.2515 — — — LNU450 63710.2 0.7 0.12 17 — — — — — — LNU450 63712.3 0.6 0.1210 7.9 0.03 18 — — — LNU429 63938.2 — — — 8.1 L 22 7.4 L 12 LNU42666150.2 — — — 7.2 0.29  8 7.2 0.04  9 LNU416 64134.11 0.7 0.05 17 8.40.08 27 7.1 0.08  7 LNU416 64134.2 — — — 9.2 L 39 7.1 0.07  8 LNU41263940.12 — — — 7.6 0.28 14 — — — LNU412 63940.8 0.7 0.22 22 9.0 0.05 357.1 0.10  7 LNU412 63941.2 — — — 7.8 0.09 17 7.1 0.08  8 LNU349 63990.2— — — 7.5 0.17 13 7.1 0.24  8 LNU349 63990.4 0.6 0.24 11 8.6 L 29 7.40.01 11 LNU293 65048.1 0.7 0.26 21 8.3 0.11 24 7.0 0.14  6 LNU29365051.3 0.7 L 24 9.2 L 39 7.4 L 12 CONT. — 0.6 — — 6.7 — — 6.6 — —LNU498 64184.3 — — — 6.6 0.23 19 7.5 0.22  8 LNU498 64185.3 0.5 0.02 30— — — — — — LNU498 64186.2 — — — 7.7 L 39 7.6 0.06 10 LNU498 64186.3 — —— — — — 7.5 0.20  9 LNU493 64191.4 — — — 7.2 0.29 31 7.7 0.30 11 LNU45564187.4 — — — 6.3 0.15 15 7.7 L 11 LNU455 64187.5 0.5 L 42 7.7 0.07 397.8 0.01 13 LNU455 64189.4 0.5 0.14 21 8.7 0.02 57 7.6 0.01 10 LNU45564189.7 — — — 6.2 0.24 11 7.6 0.01 10 LNU343 64208.1 0.4 0.19 15 — — — —— — LNU343 64208.4 — — — — — — 7.3 0.09  5 LNU343 64209.1 0.4 0.19 15 —— — — — — LNU328 64150.1 0.5 0.09 19 8.0 L 45 7.8 0.05 13 LNU328 64150.4— — — 7.2 0.13 30 7.8 L 13 LNU328 64151.1 0.4 0.28 13 6.6 0.14 19 7.50.02  8 LNU328 64151.2 — — — — — — 7.1 0.30  3 LNU322 63917.2 — — — 7.00.05 27 7.7 L 11 LNU322 63918.1 0.5 0.06 23 6.4 0.14 16 — — — LNU32263918.3 0.5 0.23 25 — — — — — — LNU317 64097.2 — — — 7.2 0.08 30 7.60.17 10 LNU305 64111.3 0.5 0.04 32 6.9 0.11 24 7.9 0.02 14 LNU30564115.1 — — — 6.3 0.17 14 7.6 L  9 CONT. — 0.4 — — 5.5 — — 6.9 — —LNU495 64697.3 — — — 5.6 0.17 16 — — — LNU495 64701.3 — — — 5.4 0.25 11— — — LNU495 64701.4 — — — 7.1 L 47 6.8 0.16  8 LNU487 64702.1 0.5 L 267.3 L 51 7.1 0.03 13 LNU487 64702.3 0.5 0.10 14 6.7 0.06 38 — — — LNU47464381.1 — — — 6.7 0.21 38 — — — LNU474 64382.2 0.5 0.12 11 5.9 0.16 22 —— — LNU474 64382.3 — — — — — — 7.0 0.03 11 LNU465 64020.1 0.5 0.24 25 —— — — — — LNU465 64022.2 — — — 6.2 0.23 27 6.6 0.24  5 LNU446 64546.2 —— — 7.0 L 45 7.1 0.02 12 LNU446 64546.3 — — — — — — 7.0 0.12 12 LNU44664549.2 — — — 6.2 0.04 29 6.7 0.21  7 LNU443 64023.2 — — — 6.4 L 33 6.90.14  9 LNU443 64024.4 — — — 6.3 0.09 31 — — — LNU443 64024.7 0.5 0.20 6 — — — — — — LNU436 64240.1 0.6 0.10 36 — — — 6.6 0.24  6 LNU43664240.2 0.5 0.02 20 — — — — — — LNU436 64241.3 — — — 6.0 0.18 25 — — —LNU379 64170.3 — — — 6.1 0.02 25 6.9 0.20  9 LNU379 64170.4 — — — 6.30.05 30 7.1 0.06 12 LNU315 64224.1 — — — 6.2 0.16 29 — — — LNU31564224.3 — — — 5.8 0.05 19 — — — LNU315 64225.1 — — — 7.6 L 58 7.4 0.0217 LNU315 64226.3 0.6 L 33 10.0  L 107  7.7 L 23 LNU315 64227.3 0.5 0.1010 5.6 0.14 17 — — — CONT. — 0.4 — — 4.8 — — 6.3 — — LNU497 64206.2 — —— 6.1 0.08 15 6.7 0.25  7 LNU491 64406.4 — — — 6.4 0.27 21 — — — LNU44964570.1 — — — — — — 6.9 0.10 11 LNU449 64571.3 0.7 0.16 28 7.8 0.10 466.8 0.12  9 LNU432 64065.2 0.7 0.17 42 10.1  0.20 90 7.4 0.17 19 LNU43264066.2 0.6 0.26 15 — — — — — — LNU432 64559.2 0.7 0.14 35 8.1 0.15 526.9 0.14 11 LNU432 64560.5 — — — 6.5 0.20 23 — — — LNU378 64495.4 — — —6.3 0.06 19 7.1 L 14 LNU367 64398.2 0.7 0.13 34 7.4 0.18 40 — — — LNU36764398.3 — — — — — — 6.6 0.23  6 LNU367 64399.1 0.6 0.22 14 — — — — — —LNU367 64399.2 0.7 0.07 39 — — — — — — LNU364 64441.3 — — — — — — 6.60.21  6 LNU362 64324.2 — — — 6.3 0.16 19 — — — CONT. — 0.5 — — 5.3 — —6.2 — — LNU495 64697.3 — — — — — — 6.5 0.06  5 LNU487 64704.2 — — — 6.60.18 20 6.8 0.04 11 LNU474 64379.1 — — — — — — 6.6 0.11  7 LNU47464382.3 — — — — — — 6.5 0.16  6 LNU474 64383.2 — — — 6.8 0.16 23 6.70.12  8 LNU446 64546.2 — — — — — — 6.5 0.02  5 LNU446 64546.3 0.6 0.2113 7.2 0.06 30 7.1 0.02 14 LNU446 64548.2 — — — — — — 6.5 0.19  5 LNU44364023.2 0.6 0.11 22 — — — 6.8 0.05 11 LNU436 64240.2 — — — — — — 6.70.09  8 LNU436 64241.3 0.7 0.02 32 7.6 0.23 37 6.8 0.26  9 LNU37964170.2 — — — — — — 6.9 0.04 12 LNU379 64170.3 0.6 0.05 20 7.3 0.03 336.8 0.06 10 LNU379 64172.2 0.7 L 44 7.8 0.02 41 6.7 0.02  7 LNU31564225.2 0.7 L 39 7.4 0.02 34 — — — LNU315 64226.3 0.6 0.02 22 — — — — —— LNU315 64227.3 0.6 0.09 20 — — — 6.9 L 12 CONT. — 0.5 — — 5.5 — — 6.2— — LNU520 64155.1 — — — — — — 6.8 0.06  8 LNU520 64156.13 — — — — — —6.7 0.12  7 LNU520 64156.7 — — — 6.5 0.19  9 — — — LNU518 64014.3 — — —— — — 6.7 0.24  6 LNU518 64015.4 — — — — — — 7.0 0.03 12 LNU502 64040.4— — — 6.8 0.29 13 6.9 0.05 10 LNU405 64159.6 — — — — — — 6.7 0.29  6LNU405 64159.8 — — — — — — 6.9 0.14 11 LNU405 64159.9 — — — — — — 7.00.05 11 LNU403 64236.3 — — — 6.5 0.21  9 6.6 0.24  5 LNU403 64236.4 — —— — — — 6.7 0.22  7 LNU393 63977.5 — — — — — — 6.9 0.05 10 LNU39363977.6 — — — — — — 6.9 0.06 11 LNU385 64245.3 — — — — — — 7.1 0.06 13LNU385 64246.3 — — — — — — 6.9 0.13  9 LNU385 64247.1 — — — — — — 6.90.03 11 LNU385 64247.2 — — — — — — 6.9 0.02 10 LNU374 63997.1 — — — — —— 6.9 0.03 10 CONT. — — — — 6.0 — — 6.3 — — LNU519 64678.1 0.5 0.01 24 —— — — — — LNU519 64679.1 0.5 0.06 11 6.7 0.02 22 — — — LNU519 64681.3 —— — 7.1 0.12 30 — — — LNU519 64681.8 0.5 0.17  7 6.8 0.05 25 — — —LNU500 64221.2 — — — 7.0 0.06 27 7.1 0.03  8 LNU500 64223.1 — — — 6.90.12 26 7.0 0.04  7 LNU500 64223.2 — — — 6.5 0.19 18 7.0 0.28  7 LNU45964542.3 0.5 L 19 7.3 L 34 7.0 0.02  7 LNU459 64543.2 — — — 6.0 0.27 11 —— — LNU348 64472.3 — — — 6.0 0.25 10 — — — LNU348 64474.1 0.5 0.09 17 —— — — — — LNU348 64474.2 0.5 0.06 18 8.0 L 46 6.9 0.11  5 LNU329 63428.1— — — — — — 6.9 0.23  5 LNU329 63428.2 0.5 0.29 14 — — — — — — LNU32963429.1 — — — 6.6 0.05 21 — — — LNU329 63430.3 0.5 0.29  5 — — — — — —CONT. — 0.4 — — 5.5 — — 6.6 — — LNU490 66096.1 — — — — — — 5.7 0.10 12LNU437_H2 66104.2 0.6 0.21 22 5.4 0.27 34 — — — LNU416 64134.11 — — — —— — 5.5 0.29  9 LNU416 64134.2 — — — — — — 5.8 0.10 15 LNU395 64142.50.6 0.07 18 4.8 0.11 19 5.4 0.28  7 LNU395 64143.6 — — — — — — 5.7 0.1413 LNU312 64000.1 — — — 5.1 0.23 25 5.6 0.05 11 LNU312 64002.3 0.6 0.0934 — — — — — — LNU312 64002.5 0.5 0.23 12 5.0 0.17 23 — — — LNU29866086.4 — — — 5.0 0.11 24 5.9 0.01 16 CONT. — 0.5 — — 4.0 — — 5.1 — —LNU468 63491.1 — — — — — — 6.7 0.20  9 LNU468 63492.2 — — — — — — 6.9 L14 LNU468 63493.4 — — — — — — 6.9 0.03 13 LNU467 63715.1 — — — — — — 6.30.30  4 LNU467 63716.1 — — — 7.6 0.07 17 — — — LNU462 63504.1 — — — — —— 6.6 0.26  8 LNU450 63708.3 — — — 10.9  0.02 68 7.1 L 16 LNU450 63708.6— — — — — — 6.4 0.28  6 LNU450 63710.2 — — — — — — 6.8 0.01 12 LNU45063712.3 — — — — — — 6.4 0.22  5 LNU448 63705.2 — — — — — — 7.1 0.04 16LNU448 63705.3 — — — 7.1 0.29  9 6.6 0.14  8 LNU448 63707.2 — — — — — —6.9 0.06 13 LNU392 63696.1 — — — — — — 6.7 0.02 10 LNU392 63701.2 0.70.13 12 — — — — — — LNU390 63539.2 — — — — — — 6.9 L 14 LNU390 63539.3 —— — — — — 6.6 0.09  9 LNU390 63539.4 — — — 8.2 0.01 26 6.7 0.06 11LNU390 63540.9 — — — 7.3 0.16 12 6.8 0.03 12 LNU347 63508.1 — — — — — —6.4 0.21  5 LNU347 63510.2 — — — 7.6 0.20 17 6.7 0.04  9 LNU347 63513.3— — — — — — 6.5 0.17  6 LNU347 63513.4 — — — — — — 6.7 0.12 10 LNU32363421.2 — — — — — — 6.5 0.14  6 LNU323 63424.4 — — — — — — 6.9 L 13CONT. — 0.6 — — 6.5 — — 6.1 — — LNU497 64206.2 — — — — — — 7.1 0.01 10LNU497 64207.2 0.6 0.02 29 — — — — — — LNU497 64207.3 — — — 6.8 0.08 376.7 0.11  4 LNU491 64404.3 0.7 L 52 7.8 0.04 56 7.5 0.02 16 LNU49164404.6 0.6 0.02 28 6.4 0.25 28 7.0 0.23  8 LNU491 64406.4 — — — 6.00.11 20 7.2 0.02 12 LNU449 63890.1 — — — 6.3 0.16 25 7.0 0.19  9 LNU43264065.2 0.6 0.15 23 — — — — — — LNU432 64066.2 0.7 0.02 52 6.5 0.18 31 —— — LNU432 64559.2 — — — 5.8 0.20 17 6.9 0.04  7 LNU432 64560.5 — — —5.9 0.20 19 — — — LNU367 64398.2 0.6 0.12 18 — — — — — — CONT. — 0.5 — —5.0 — — 6.4 — — LNU510 64152.1 — — — 8.1 0.02 29 7.6 L 25 LNU510 64153.5— — — — — — 7.0 0.11 15 LNU489 64011.1 — — — — — — 7.2 0.05 20 LNU48964012.1 — — — 7.5 0.06 19 7.7 L 27 LNU489 64013.1 — — — 7.9 0.21 26 7.60.03 26 LNU438 63994.12 0.7 0.23 16 8.4 0.25 33 7.2 0.15 19 LNU43863994.2 — — — 7.8 0.05 23 7.7 L 28 LNU438 63994.3 — — — 7.4 0.29 18 7.40.02 22 LNU438 63994.5 — — — 9.1 0.06 44 8.1 0.01 33 LNU427 64178.6 — —— — — — 7.1 0.13 16 LNU427 64180.3 — — — — — — 7.1 0.05 17 LNU42764180.4 — — — — — — 6.6 0.24  9 LNU383 63982.1 — — — 8.2 0.02 30 7.9 L31 LNU383 63982.7 — — — 8.1 0.13 29 7.9 L 30 LNU354 63970.6 — — — 7.70.21 22 7.7 L 27 LNU354 63972.5 — — — — — — 6.6 0.26 10 LNU354 63972.8 —— — — — — 7.5 L 24 LNU310 63904.1 — — — — — — 7.1 0.04 17 LNU310 63904.3— — — 9.2 0.07 45 7.5 0.01 24 LNU310 63905.3 — — — — — — 6.7 0.28 10LNU299 64326.2 — — — 9.1 0.09 45 7.6 0.01 25 LNU299 64327.2 — — — — — —6.8 0.13 12 LNU299 64328.2 — — — — — — 6.8 0.16 12 LNU295 63899.5 — — —9.3 0.06 47 7.5 L 24 LNU295 63899.8 — — — — — — 6.7 0.29 11 LNU29563901.3 — — — 7.6 0.11 20 7.2 0.03 19 LNU295 63902.3 — — — — — — 7.50.01 25 CONT. — 0.6 — — 6.3 — — 6.1 — — LNU483 64803.2 0.6 0.05 30 9.00.01 48 — — — LNU483 64803.3 0.7 L 41 9.0 L 50 — — — LNU483 64805.1 0.60.15 22 8.7 0.10 44 — — — LNU483 64805.2 0.7 L 34 9.2 0.01 53 — — —LNU483 64806.2 — — — 8.6 L 42 — — — LNU464 65076.4 0.6 0.19 19 — — — — —— LNU435 64463.3 0.7 0.06 37 8.4 0.08 38 — — — LNU346 65007.3 — — — — —— 7.3 0.04  7 LNU346 65008.2 0.5 0.22 12 — — — — — — CONT. — 0.5 — — 6.0— — 6.8 — — LNU510 64153.5 — — — 5.9 0.12 23 7.1 L 12 LNU489 64010.8 0.50.26 14 6.3 0.09 33 — — — LNU489 64012.1 — — — 5.9 0.18 24 6.8 0.01  8LNU489 64013.1 — — — — — — 6.8 0.07  7 LNU438 63994.1 — — — 7.4 0.03 557.7 L 22 LNU438 63994.12 — — — 6.6 0.06 38 7.3 0.01 15 LNU438 63994.2 —— — 7.0 0.01 47 7.5 0.01 19 LNU438 63994.5 0.5 0.17 28 — — — — — —LNU427 64180.4 — — — 6.4 0.02 34 7.1 0.03 12 LNU383 63982.1 0.5 0.16 185.6 0.22 17 — — — LNU354 63970.6 — — — 6.5 0.01 35 7.0 0.01 11 LNU35463971.5 — — — 6.4 0.09 33 7.3 0.12 15 LNU354 63972.5 — — — 5.8 0.09 227.0 0.19 10 LNU354 63972.8 0.5 0.19 17 8.1 L 69 7.7 0.01 22 LNU31063904.3 — — — 6.9 L 44 7.2 0.03 14 LNU310 63905.1 — — — 6.3 0.25 31 7.00.19 11 LNU310 63905.3 — — — — — — 6.9 0.06  9 LNU299 64327.2 — — — 6.7L 40 7.2 L 14 LNU299 64330.5 — — — 7.2 0.15 51 — — — LNU295 63899.5 0.50.09 23 7.0 L 46 7.3 L 15 LNU295 63899.8 0.5 0.28 19 8.6 0.06 81 7.50.07 18 LNU295 63901.3 — — — 5.7 0.27 19 7.1 0.13 12 LNU295 63902.3 0.60.11 51 9.7 0.08 102  7.6 0.05 20 CONT. — 0.4 — — 4.8 — — 6.3 — — LNU49066093.2 0.5 0.16 19 5.0 0.01 56 6.1 0.08 12 LNU490 66096.1 — — — 4.80.03 50 6.6 L 22 LNU443 64023.2 — — — 4.7 L 47 5.7 0.16  6 LNU44364024.3 — — — 3.8 0.27 18 — — — LNU443 64024.4 0.5 0.21 15 5.6 0.01 756.2 0.03 15 LNU443 64024.7 0.6 L 59 5.4 L 70 6.3 0.03 16 LNU439 64614.40.5 0.12 36 5.1 0.06 61 — — — LNU439 64616.2 — — — 3.6 0.25 14 — — —LNU439 64618.3 0.6 0.20 48 5.9 0.08 86 6.4 L 19 LNU437_H2 66104.1 — — —4.6 0.04 45 6.0 0.06 12 LNU437_H2 66104.2 — — — 5.3 0.01 66 5.8 0.12  7LNU436 64240.1 — — — 4.6 L 46 5.8 0.16  8 LNU436 64240.2 0.5 0.08 33 4.40.07 40 6.1 0.12 13 LNU436 64241.3 — — — 3.6 0.25 14 — — — LNU43664242.2 0.5 0.06 28 4.7 0.15 48 6.0 0.22 10 LNU436 64243.1 — — — 4.60.09 44 5.9 0.17  9 LNU433 64815.2 0.5 0.03 28 6.0 0.04 89 — — — LNU31166099.1 — — — 5.5 0.09 73 6.2 0.12 15 LNU311 66099.2 — — — 4.2 0.05 316.4 L 18 LNU311 66100.3 — — — 3.7 0.29 15 5.8 0.21  7 LNU298 66086.4 — —— 4.7 L 48 6.0 0.05 12 LNU298 66088.3 — — — 5.0 L 56 — — — LNU29866089.1 — — — 3.9 0.15 23 — — — LNU298 66089.3 — — — 4.5 0.07 40 5.90.22  9 LNU293 65048.1 — — — 5.1 0.01 60 6.4 0.01 18 LNU293 65049.1 — —— 4.0 0.07 27 — — — LNU293 65050.3 — — — 3.9 0.19 21 — — — LNU29365051.3 0.5 0.08 21 4.7 0.04 47 6.1 0.11 13 CONT. — 0.4 — — 3.2 — — 5.4— — Table 76: “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant.

TABLE 77 Genes showing improved plant performance at standard nitrogengrowth conditions (T1 generation) Gene Dry Weight [mg] Fresh Weight [mg]Name Ave. P-Val. % Incr. Ave. P-Val. % Incr. LNU488 45.8 0.11 26 100.70.06 32 LNU359 60.2 0.04 66 109.6 L 44 LNU358 — — — 98.4 0.11 29 LNU341— — — 90.3 0.04 18 LNU309_H3 — — — 96.2 0.05 26 CONT. 36.3 — — 76.3 — —Table 77: “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant.

TABLE 78 Genes showing improved plant performance at standard nitrogengrowth conditions (T1 generation) Leaf Area [cm²] Roots Coverage [cm²]Roots Length [cm²] P- % P- % P- % Gene Name Ave. Val. Incr. Ave. Val.Incr. Ave. Val. Incr. LNU490 — — — 2.3 0.12 35 3.7 0.03 29 LNU417_H4 — —— 2.1 0.28 24 3.6 0.10 27 LNU394 — — — 2.2 0.19 30 3.6 0.07 26 CONT. — —— 1.7 — — 2.8 — — LNU488 0.6 0.01 34 5.4 L 96 6.1 L 46 LNU466 — — — 3.80.04 36 5.1 0.04 21 LNU453 0.5 0.08 16 3.4 0.09 24 4.9 0.04 18 LNU3590.6 0.05 45 5.5 0.01 98 5.9 L 42 LNU358 0.5 0.19 21 4.0 L 46 5.2 0.08 25LNU341 0.5 0.07 33 — — — — — — LNU309_H3 0.6 0.11 33 4.9 0.17 75 5.90.02 40 CONT. 0.4 — — 2.8 — — 4.2 — — Table 78: “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant.

The genes listed in Tables 79-80 improved plant relative growth rate(RGR of leaf area, root length and root coverage) when grown at standardnitrogen concentration levels. These produced plants that grew fasterthan control plants when grown under standard nitrogen growthconditions. Faster growth was observed when growth rate of leaf area androot length and coverage was measured.

TABLE 79 Genes showing improved growth rate at standard nitrogen growthconditions (T2 generation) RGR Of RGR Of Root RGR Of Roots Leaf AreaCoverage Length P- % P- % P- % Gene Name Event # Ave. Val. Incr. Ave.Val. Incr. Ave. Val. Incr. LNU437_H2 66104.1 — — — — — — 0.6 0.02 19LNU437_H2 66104.2 0.0 0.02 42 — — — 0.6 0.26  8 LNU437_H2 66104.3 0.1 L54 — — — 0.6 0.06 15 LNU426 66147.3 0.1 L 77 — — — 0.6 0.09 14 LNU42064008.4 0.0 0.14 25 — — — — — — LNU352 64199.1 0.1 L 85 — — — 0.6 0.0219 LNU352 64200.1 0.0 0.15 26 — — — — — — LNU352  64200.10 0.0 0.14 26 —— — 0.6 0.04 16 LNU352 64200.4 — — — — — — 0.6 0.09 12 LNU292 64085.4 —— — — — — 0.6 0.06 13 CONT. — 0.0 — — — — — 0.5 — — LNU483 64803.2 0.10.09 17 — — — 0.6 0.29 14 LNU483 64803.3 0.1 0.15 17 — — — — — — LNU48364805.2 0.1 0.30 12 — — — — — — LNU477 63886.1 0.1 0.05 21 — — — — — —LNU447 65000.1 0.1 0.05 23 — — — — — — LNU447 65002.3 0.1 0.11 20 — — —— — — LNU447 65004.1 0.1 0.16 19 — — — — — — LNU439 64614.4 — — — — — —0.7 0.16 17 LNU439 64616.2 0.1 0.03 21 — — — — — — LNU439 64616.3 0.1 L41 — — — 0.7 0.10 22 LNU439 64618.3 0.1 0.13 23 — — — — — — LNU425 63911.11 0.1 0.25 13 — — — — — — LNU425 63911.9 0.1 0.24 14 — — — — — —LNU414 64475.1 0.1 0.04 34 — — — — — — LNU336 64447.2 0.1 0.02 32 — — —— — — LNU336 64448.2 0.1 0.22 29 — — — — — — LNU336 64448.3 0.1 0.18 17— — — — — — LNU336 64449.4 0.1 0.12 17 — — — — — — CONT. — 0.1 — — — — —0.6 — — LNU473 65770.4 — — — — — — 0.7 0.23  8 LNU473 65771.3 — — — — —— 0.7 0.22  9 LNU470 64228.3 0.1 0.20 14 — — — 0.7 0.02 17 LNU47064229.1 — — — — — — 0.7 0.03 19 LNU460 64359.3 0.1 L 39 — — — 0.8 L 23LNU460 64362.1 — — — — — — 0.7 0.22  8 LNU421 64302.7 — — — — — — 0.70.28  7 LNU421 64303.3 — — — — — — 0.7 0.12 12 LNU408  64248.10 — — — —— — 0.7 0.02 16 LNU408  64248.12 — — — — — — 0.7 0.16 12 LNU408 64250.80.1 0.21 13 — — — 0.7 0.04 16 LNU380 65764.2 — — — — — — 0.7 0.05 13LNU380 65764.3 — — — — — — 0.7 0.08 13 LNU380 65765.4 — — — — — — 0.70.06 17 LNU340  64290.11 — — — — — — 0.8 L 21 LNU340 64290.7 — — — — — —0.8 L 25 LNU340  64291.10 — — — — — — 0.7 L 19 LNU331 64214.2 — — — — —— 0.7 0.26  8 LNU331 64215.1 — — — — — — 0.7 0.18 11 LNU331 64215.3 — —— — — — 0.7 0.04 14 LNU306 64131.2 — — — — — — 0.7 0.09 13 CONT. — 0.1 —— — — — 0.6 — — LNU456 63991.2 — — — — — — 0.7 0.02 19 LNU456 63991.8 —— — — — — 0.6 0.02 16 LNU430 63934.3 — — — — — — 0.6 0.27 10 LNU43063936.1 — — — — — — 0.6 0.13 12 LNU412 63940.1 — — — — — — 0.6 0.16  9LNU412 63941.2 — — — — — — 0.6 0.19  9 LNU407 64218.2 — — — — — — 0.60.05 13 LNU407 64219.1 — — — — — — 0.6 0.01 17 LNU402 63913.1 — — — — —— 0.6 0.14 10 LNU384 64161.3 — — — — — — 0.7 L 27 LNU384 64161.7 — — — —— — 0.7 0.01 22 LNU360 64029.2 — — — — — — 0.6 0.26  8 LNU360 64029.3 —— — — — — 0.6 0.27  9 LNU360 64030.4 — — — — — — 0.7 L 24 LNU360 64030.6— — — — — — 0.6 0.11 11 LNU335  64168.15 — — — — — — 0.6 0.22  8 LNU33564169.2 — — — — — — 0.6 0.23  8 LNU301 63927.5 — — — — — — 0.6 0.03 18LNU301 63950.3 — — — — — — 0.7 L 19 CONT. — — — — — — — 0.6 — — LNU45063710.2 0.1 0.24 14 — — — — — — LNU450 63712.3 0.1 0.26 12 — — — — — —LNU429 63938.2 — — — — — — 0.7 0.12 15 LNU426 66150.2 — — — — — — 0.60.25 11 LNU416  64134.11 0.1 0.15 16 — — — 0.7 0.21 12 LNU416 64134.20.1 0.25 15 — — — — — — LNU416 64134.5 — — — — — — 0.7 0.19 12 LNU41263940.8 0.1 0.10 24 — — — — — — LNU359 66154.5 — — — — — — 0.7 0.17 13LNU349 63990.4 0.1 0.15 17 — — — 0.7 0.28 12 LNU293 65048.1 0.1 0.12 24— — — — — — LNU293 65051.3 0.1 0.03 25 — — — — — — CONT. — 0.1 — — — — —0.6 — — LNU498 64185.3 0.0 0.05 33 — — — — — — LNU498 64186.2 — — — — —— 0.7 0.13  8 LNU493 64191.4 — — — — — — 0.7 0.16 11 LNU455 64187.4 — —— — — — 0.7 0.06  9 LNU455 64187.5 0.1 0.01 45 — — — 0.7 0.07 11 LNU45564189.4 0.0 0.09 30 — — — — — — LNU455 64189.7 — — — — — — 0.7 0.08  9LNU343 64208.1 0.0 0.29 18 — — — — — — LNU343 64208.4 — — — — — — 0.70.06  9 LNU343 64209.1 0.0 0.27 18 — — — — — — LNU328 64150.1 0.0 0.1623 — — — — — — LNU328 64150.4 — — — — — — 0.7 0.10  9 LNU328 64151.2 — —— — — — 0.7 0.10  8 LNU322 63917.2 0.0 0.23 20 — — — 0.8 L 18 LNU32263918.1 0.0 0.19 21 — — — — — — LNU322 63918.3 0.0 0.16 30 — — — — — —LNU317 64097.2 — — — — — — 0.7 0.11 12 LNU305 64111.3 0.0 0.08 31 — — —0.7 0.02 13 CONT. — 0.0 — — — — — 0.7 — — LNU495 64701.4 0.0 0.13 17 — —— 0.6 0.14 17 LNU487 64702.1 0.1 L 35 — — — 0.6 0.25 13 LNU487 64702.30.0 0.09 18 — — — — — — LNU487 64706.2 — — — — — — 0.6 0.16 15 LNU47464381.1 0.0 0.15 17 — — — — — — LNU474 64382.2 0.0 0.02 22 — — — — — —LNU474 64382.3 — — — — — — 0.6 0.16 15 LNU465 64020.1 0.1 0.07 30 — — —— — — LNU446 64546.2 0.0 0.16 14 — — — — — — LNU446 64546.3 — — — — — —0.6 0.26 12 LNU443 64024.3 0.1 L 38 — — — — — — LNU436 64240.1 0.1 0.0237 — — — — — — LNU436 64240.2 0.0 0.04 21 — — — — — — LNU436 64242.2 0.10.02 56 — — — 0.7 0.13 29 LNU436 64243.1 0.0 0.18 18 — — — — — — LNU37964170.3 — — — — — — 0.6 0.10 18 LNU315 64224.1 0.0 0.27 14 — — — — — —LNU315 64224.3 0.0 0.24 13 — — — — — — LNU315 64225.1 0.0 0.14 22 — — —0.6 0.07 23 LNU315 64226.3 0.1 L 37 — — — 0.7 0.04 28 LNU315 64227.3 0.00.20 13 — — — — — — CONT. — 0.0 — — — — — 0.5 — — LNU491 64406.4 0.10.18 29 — — — — — — LNU449 64571.3 0.1 0.13 31 — — — 0.6 0.25 10 LNU43264065.2 0.1 0.11 44 — — — 0.6 0.21 17 LNU432 64559.2 0.1 0.06 42 — — —0.6 0.10 16 LNU432 64560.5 — — — — — — 0.6 0.11 14 LNU367 64398.2 0.10.13 33 — — — — — — LNU367 64399.1 0.1 0.26 19 — — — — — — LNU36764399.2 0.1 0.08 38 — — — — — — LNU364 64441.3 — — — — — — 0.6 0.25  9CONT. — 0.0 — — — — — 0.6 — — LNU495 64697.3 — — — — — — 0.6 L 24 LNU49564701.3 — — — — — — 0.5 0.25 10 LNU487 64702.1 — — — — — — 0.6 0.11 14LNU487 64702.3 — — — — — — 0.5 0.30  9 LNU487 64704.2 0.1 0.26 16 — — —0.6 0.05 18 LNU487 64705.4 — — — — — — 0.5 0.16 11 LNU487 64706.2 — — —— — — 0.5 0.10 13 LNU474 64379.1 — — — — — — 0.6 0.01 24 LNU474 64382.3— — — — — — 0.6 0.13 14 LNU474 64383.2 — — — — — — 0.6 0.05 18 LNU46564022.2 — — — — — — 0.5 0.17 10 LNU446 64546.2 — — — — — — 0.5 0.18  9LNU446 64546.3 0.1 0.12 18 — — — 0.6 0.07 19 LNU446 64548.1 — — — — — —0.5 0.26 12 LNU443 64023.2 0.1 0.06 25 — — — 0.6 0.02 21 LNU436 64240.2— — — — — — 0.6 0.06 16 LNU436 64241.3 0.1 L 35 — — — 0.6 0.05 23 LNU37964170.2 — — — — — — 0.6 0.04 21 LNU379 64170.3 0.1 0.04 24 — — — 0.6 L30 LNU379 64172.2 0.1 L 51 — — — 0.6 L 25 LNU315 64225.2 0.1 L 40 — — —0.6 0.04 20 LNU315 64226.3 0.1 0.02 25 — — — 0.6 0.04 18 LNU315 64227.30.1 0.11 19 — — — 0.6 0.04 17 CONT. — 0.0 — — — — — 0.5 — — LNU50264040.4 — — — — — — 0.6 0.25 11 CONT. — — — — — — — 0.5 — — LNU51964678.1 0.1 L 25 — — — — — — LNU519 64679.1 0.0 0.12 10 — — — — — —LNU519 64681.3 — — — — — — 0.7 0.09 13 LNU519 64681.8 0.0 0.11 10 — — —— — — LNU500 64223.1 — — — — — — 0.7 0.22  9 LNU500 64223.2 — — — — — —0.7 0.15 12 LNU459 64542.3 0.1 L 23 — — — 0.7 0.06 14 LNU348 64474.1 0.10.05 16 — — — — — — LNU348 64474.2 0.1 0.01 20 — — — 0.7 0.20 10 LNU32963428.1 — — — — — — 0.7 0.10 11 LNU329 63428.2 0.0 0.27 11 — — — — — —LNU329 63429.1 0.1 0.20 13 — — — — — — LNU316 64565.4 — — — — — — 0.70.17 10 CONT. — 0.0 — — — — — 0.6 — — LNU490 66093.2 — — — — — — 0.50.10 15 LNU490 66095.2 — — — — — — 0.5 0.02 24 LNU437_H2 66104.1 — — — —— — 0.5 0.20 17 LNU437_H2 66104.2 0.1 0.12 25 — — — 0.5 0.07 21LNU437_H2 66104.3 — — — — — — 0.5 0.29 12 LNU416  64134.11 — — — — — —0.5 0.11 18 LNU416 64134.2 — — — — — — 0.5 0.01 27 LNU416 64136.4 — — —— — — 0.5 0.10 16 LNU395 64142.5 0.1 0.11 21 — — — — — — LNU395 64143.60.1 0.29 13 — — — 0.5 L 32 LNU312 64000.1 — — — — — — 0.5 0.02 21 LNU31264002.2 — — — — — — 0.5 0.23 15 LNU312 64002.3 0.1 0.04 34 — — — — — —LNU312 64002.5 — — — — — — 0.5 0.09 17 LNU311 66099.1 — — — — — — 0.50.08 17 LNU298 66086.4 — — — — — — 0.5 L 27 LNU298 66089.1 — — — — — —0.5 0.17 13 CONT. — 0.0 — — — — — 0.4 — — LNU468 63491.1 — — — — — — 0.60.10 16 LNU468 63492.2 — — — — — — 0.6 0.06 16 LNU468 63493.4 — — — — —— 0.6 0.13 14 LNU467 63715.1 — — — — — — 0.6 0.25  9 LNU462 63505.1 — —— — — — 0.6 0.14 12 LNU450 63708.3 — — — — — — 0.7 0.01 24 LNU45063710.2 — — — — — — 0.6 0.10 14 LNU448 63705.2 — — — — — — 0.7 L 26LNU448 63705.3 — — — — — — 0.6 0.27 10 LNU448 63707.2 — — — — — — 0.60.04 20 LNU392 63696.1 — — — — — — 0.6 0.08 15 LNU392 63697.4 — — — — —— 0.6 0.16 12 LNU392 63698.2 — — — — — — 0.6 0.07 18 LNU390 63539.4 — —— — — — 0.6 0.17 12 LNU347 63508.1 — — — — — — 0.6 0.29  9 LNU34763510.2 — — — — — — 0.6 0.01 23 LNU347 63510.4 — — — — — — 0.6 0.18 14LNU347 63513.3 — — — — — — 0.6 0.08 15 LNU347 63513.4 — — — — — — 0.60.03 21 LNU323 63421.2 — — — — — — 0.6 0.23 10 LNU323 63424.4 — — — — —— 0.6 0.04 18 CONT. — — — — — — — 0.5 — — LNU497 64206.2 — — — — — — 0.60.02 16 LNU497 64207.2 0.1 0.18 22 — — — — — — LNU491 64403.1 — — — — —— 0.6 0.17 11 LNU491 64404.3 0.1 L 47 — — — — — — LNU491 64404.6 0.10.18 22 — — — — — — LNU491 64406.4 — — — — — — 0.6 0.15 11 LNU44963890.1 — — — — — — 0.6 0.25 12 LNU432 64066.2 0.1 0.01 48 — — — — — —LNU432 64559.2 — — — — — — 0.6 0.20  9 LNU432 64560.5 — — — — — — 0.60.05 14 CONT. — 0.0 — — — — — 0.6 — — LNU510 64152.1 — — — — — — 0.70.06 34 LNU489 64011.1 — — — — — — 0.6 0.23 22 LNU489 64012.1 — — — — —— 0.7 0.06 34 LNU489 64013.1 — — — — — — 0.7 0.13 28 LNU438  63994.12 —— — — — — 0.6 0.26 22 LNU438 63994.2 — — — — — — 0.7 0.04 37 LNU43863994.3 — — — — — — 0.7 0.12 28 LNU438 63994.5 — — — — — — 0.7 0.04 40LNU427 64178.6 — — — — — — 0.7 0.21 23 LNU427 64180.3 — — — — — — 0.70.18 24 LNU383 63982.1 — — — — — — 0.7 0.05 35 LNU383 63982.7 — — — — —— 0.7 0.06 35 LNU354 63970.6 — — — — — — 0.7 0.06 33 LNU354 63972.8 — —— — — — 0.7 0.22 23 LNU310 63904.1 — — — — — — 0.7 0.15 26 LNU31063904.3 0.1 0.18 17 — — — 0.7 0.15 28 LNU299 64326.2 — — — — — — 0.70.16 26 LNU299 64328.2 — — — — — — 0.6 0.28 20 LNU295 63899.5 — — — — —— 0.6 0.21 22 LNU295 63901.3 — — — — — — 0.7 0.11 29 LNU295 63902.3 — —— — — — 0.7 0.17 29 CONT. — 0.1 — — — — — 0.5 — — LNU483 64803.2 0.10.11 27 — — — — — — LNU483 64803.3 0.1 0.02 41 — — — — — — LNU48364805.2 0.1 0.06 31 — — — — — — LNU435 64463.3 0.1 0.05 36 — — — — — —LNU378 64494.2 — — — — — — 0.7 0.21 14 LNU346 65007.3 — — — — — — 0.70.20  8 CONT. — 0.0 — — — — — 0.6 — — LNU510 64153.5 — — — — — — 0.70.02 12 LNU489 64010.8 0.0 0.28 23 — — — 0.7 0.08 13 LNU489 64012.1 — —— — — — 0.6 0.05 10 LNU438 63994.1 — — — — — — 0.7 L 25 LNU438  63994.12— — — — — — 0.7 L 17 LNU438 63994.2 — — — — — — 0.7 L 20 LNU438 63994.50.1 0.13 39 — — — — — — LNU427 64180.4 — — — — — — 0.7 0.04 11 LNU35463970.6 — — — — — — 0.7 L 15 LNU354 63971.5 — — — — — — 0.7 0.02 19LNU354 63972.5 — — — — — — 0.7 0.11 11 LNU354 63972.8 0.0 0.21 26 — — —0.7 L 19 LNU310 63904.1 0.0 0.28 26 — — — — — — LNU310 63904.3 — — — — —— 0.7 L 17 LNU310 63905.1 — — — — — — 0.7 0.02 18 LNU310 63905.3 — — — —— — 0.6 0.07 10 LNU299 64327.2 — — — — — — 0.7 0.01 13 LNU295 63899.5 —— — — — — 0.6 0.11  8 LNU295 63899.8 0.0 0.25 27 — — — 0.7 L 21 LNU29563901.3 — — — — — — 0.7 0.09 11 LNU295 63902.3 0.1 0.03 60 — — — 0.70.01 21 CONT. — 0.0 — — — — — 0.6 — — LNU490 66093.2 0.0 0.26 20 — — —0.5 0.14 19 LNU490 66096.1 — — — — — — 0.5 0.11 18 LNU443 64023.2 — — —— — — 0.5 0.21 13 LNU443 64024.4 — — — — — — 0.5 0.02 26 LNU443 64024.70.1 0.01 50 — — — 0.5 0.05 22 LNU439 64614.4 0.1 0.08 38 — — — — — —LNU439 64618.3 0.1 0.07 52 — — — 0.5 0.08 21 LNU437_H2 66104.1 — — — — —— 0.5 0.19 15 LNU437_H2 66104.2 — — — — — — 0.5 0.18 13 LNU436 64240.20.1 0.14 29 — — — 0.5 0.08 22 LNU436 64242.2 0.0 0.26 21 — — — — — —LNU436 64243.1 — — — — — — 0.5 0.02 27 LNU433 64815.2 0.0 0.13 27 — — —0.5 0.25 14 LNU311 66099.1 — — — — — — 0.5 0.02 31 LNU311 66099.2 — — —— — — 0.5 0.04 21 LNU311 66100.3 — — — — — — 0.5 0.24 12 LNU298 66086.4— — — — — — 0.5 0.10 18 LNU298 66088.3 — — — — — — 0.5 0.10 17 LNU29866089.3 — — — — — — 0.5 0.22 15 LNU293 65048.1 — — — — — — 0.5 L 28LNU293 65051.3 0.0 0.16 25 — — — 0.5 0.04 24 CONT. — 0.0 — — — — — 0.4 —— Table 79: “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment“p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant.

TABLE 80 Genes showing improved growth rate at standard nitrogen growthconditions (T1 generation) RGR Of Leaf Area RGR Of Root Coverage RGR OfRoots Length Gene Name Ave. P-Val. % Incr. Ave. P-Val. % Incr. Ave.P-Val. % Incr. LNU490 — — — — — — 0.4 0.05 29 LNU417_H4 — — — — — — 0.40.12 27 LNU394 — — — — — — 0.4 0.09 27 CONT. — — — — — — 0.3 — — LNU4880.1 L 49 — — — 0.7 L 55 LNU466 — — — — — — 0.5 L 26 LNU453 0.0 0.11 20 —— — 0.5 0.01 25 LNU359 0.1 L 66 — — — 0.7 L 52 LNU358 0.1 0.04 34 — — —0.6 0.01 33 LNU341 0.1 0.02 36 — — — 0.5 0.13 27 LNU309_H3 0.1 0.02 44 —— — 0.6 L 45 CONT. 0.0 — — — — — 0.4 — — Table 80. “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant.

Example 17 Evaluation of Transgenic Arabidopsis NUE, Yield and PlantGrowth Rate Under Low or Normal Nitrogen Fertilization in GreenhouseAssay

Assay 1: Nitrogen Use efficiency: Seed yield plant biomass and plantgrowth rate at limited and optimal nitrogen concentration undergreenhouse conditions—This assay follows seed yield production, thebiomass formation and the rosette area growth of plants grown in thegreenhouse at limiting and non-limiting nitrogen growth conditions.Transgenic Arabidopsis seeds were sown in agar media supplemented with ½MS medium and a selection agent (Kanamycin). The T₂ transgenic seedlingswere then transplanted to 1.7 trays filled with peat and perlite in a1:1 ratio. The trays were irrigated with a solution containing nitrogenlimiting conditions, which were achieved by irrigating the plants with asolution containing 1.5 mM inorganic nitrogen in the form of KNO₃,supplemented with 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂ andmicroelements, while normal nitrogen levels were achieved by applying asolution of 6 mM inorganic nitrogen also in the form of KNO₃ with 1 mMKH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements. All plants were grownin the greenhouse until mature seeds. Seeds were harvested, extractedand weight. The remaining plant biomass (the above ground tissue) wasalso harvested, and weighted immediately or following drying in oven at50° C. for 24 hours.

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying the35S promoter and the selectable marker was used as control.

The plants were analyzed for their overall size, growth rate, flowering,seed yield, 1,000-seed weight, dry matter and harvest index (HI-seedyield/dry matter). Transgenic plants performance was compared to controlplants grown in parallel under the same conditions. Mock-transgenicplants expressing the uidA reporter gene (GUS-Intron) or with no gene atall, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital imaging—A laboratory image acquisition system, which consists ofa digital reflex camera (Canon EOS 300D) attached with a 55 mm focallength lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which includes 4 light units (4×150 Watts light bulb) isused for capturing images of plant samples.

The image capturing process is repeated every 2 days starting from day 1after transplanting till day 15. Same camera, placed in a custom madeiron mount, is used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. The tubs are squareshape include 1.7 liter trays. During the capture process, the tubs areplaced beneath the iron mount, while avoiding direct sun light andcasting of shadows.

An image analysis system is used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Imagesare captured in resolution of 10 Mega Pixels (3888×2592 pixels) andstored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data is saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data is calculated,including leaf number, rosette area, rosette diameter, leaf blade area.

Vegetative growth rate: the relative growth rate (RGR) of leaf number[formula XII (described above)], rosette area (formula V, above), plotcoverage (Formula XIX, below) and harvest index (Formula IV, above) iscalculated with the indicated formulas.

Relative growth rate of plot coverage=Regression coefficient of plotcoverage along time course.  Formula XIX

Seeds average weight—At the end of the experiment all seeds arecollected. The seeds are scattered on a glass tray and a picture wastaken. Using the digital analysis, the number of seeds in each sample iscalculated.

Dry weight and seed yield—On about day 80 from sowing, the plants areharvested and left to dry at 30° C. in a drying chamber. The biomass andseed weight of each plot are measured and divided by the number ofplants in each plot. Dry weight=total weight of the vegetative portionabove ground (excluding roots) after drying at 30° C. in a dryingchamber; Seed yield per plant=total seed weight per plant (gr). 1000seed weight (the weight of 1000 seeds) (gr.).

The harvest index (HI) was calculated using Formula IV as describedabove.

Oil percentage in seeds—At the end of the experiment all seeds from eachplot are collected. Seeds from 3 plots are mixed grounded and thenmounted onto the extraction chamber. 210 ml of n-Hexane (Cat No. 080951Biolab Ltd.) are used as the solvent. The extraction is performed for 30hours at medium heat 50° C. Once the extraction has ended the n-Hexanewas evaporated using the evaporator at 35° C. and vacuum conditions. Theprocess is repeated twice. The information gained from the Soxhletextractor (Soxhlet, F. Die gewichtsanalytische Bestimmung desMilchfettes, Polytechnisches J. (Dingler's) 1879, 232, 461) is used tocreate a calibration curve for the Low Resonance NMR. The content of oilof all seed samples is determined using the Low Resonance NMR (MARANUltra-Oxford Instrument) and its MultiQuant software package.

Silique length analysis—On day 50 from sowing, 30 siliques fromdifferent plants in each plot are sampled in block A. The chosensiliques are green-yellow in color and are collected from the bottomparts of a grown plant's stem. A digital photograph is taken todetermine silique's length.

Statistical analyses—To identify genes conferring significantly improvedtolerance to abiotic stresses, the results obtained from the transgenicplants are compared to those obtained from control plants. To identifyoutperforming genes and constructs, results from the independenttransformation events tested are analyzed separately. Data is analyzedusing Student's t-test and results are considered significant if the pvalue was less than 0.]. The JMP statistics software package is used(Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Tables 81-90 summarize the observed phenotypes of transgenic plantsexogenously expressing the gene constructs using the greenhouse seedmaturation (GH-SM) assays under low nitrogen (Tables 81-85) or normalnitrogen (Tables 86-90) conditions. The evaluation of each gene wasperformed by testing the performance of different number of events.Event with p-value<0.1 was considered statistically significant.

TABLE 81 Genes showing improved plant performance at low Nitrogen growthconditions under regulation of At6669 promoter Dry Weight Inflorescence[mg] Flowering Emergence Gene P- % P- % P- % Name Event # Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LNU519 64681.8 238.8 0.10  8 — — —— — — LNU459 64542.4 239.2 0.29  8 — — — — — — LNU409 64687.2 268.8 L 22— — — — — — LNU408 64250.8 231.9 0.30  5 — — — — — — LNU385 64245.3244.4 0.24 11 — — — — — — LNU385 64245.5 251.9 L 14 — — — — — — LNU36064029.2 237.5 0.25  8 — — — — — — LNU348 64472.2 265.6 L 20 — — — — — —LNU340 64292.5 243.1 0.07 10 — — — — — — LNU336 64449.4 — — — 14.6 0.08−10  10.8 0.01 −14  LNU331 64212.1 249.4 0.05 13 — — — — — — LNU33164214.2 242.5 0.29 10 — — — — — — LNU331 64215.3 240.6 0.05  9 — — — — —— LNU327 64491.2 245.0 0.02 11 — — — — — — LNU316 64565.3 245.6 0.02 11— — — — — — LNU290 64368.4 245.6 0.03 11 — — — — — — CONT. — 220.8 — —16.3 — — 12.5 — — LNU502 64038.5 229.4 0.04 19 15.8 0.17 −2 11.2 0.04 −6LNU502 64039.4 — — — — — — 11.5 0.12 −4 LNU500 64221.6 — — — 15.6 0.04−3 11.3 0.22 −5 LNU500 64222.1 212.5 0.12 10 — — — — — — LNU498 64185.3216.2 0.15 12 — — — — — — LNU493 64190.3 — — — 15.9 0.28 −1 — — — LNU49364191.3 226.9 0.15 18 15.7 0.24 −2 11.2 0.03 −6 LNU485 63825.1 308.1 L60 — — — 11.2 0.04 −6 LNU456 63991.2 209.4 0.16  9 — — — 11.1 L −7LNU456 63991.8 218.1 0.09 13 15.9 0.28 −1 11.5 0.12 −4 LNU455 64187.4210.0 0.29  9 15.9 0.28 −1 11.4 0.18 −5 LNU455 64187.5 — — — 15.8 0.17−2 — — — LNU455 64189.2 — — — 15.8 0.17 −2 — — — LNU384 64161.2 — — — —— — 11.5 0.12 −4 LNU384 64161.6 226.9 0.07 18 — — — — — — LNU384 64161.9204.4 0.30  6 — — — — — — LNU371 63974.6 — — — — — — 11.2 0.04 −6 LNU37163975.1 221.9 0.03 15 — — — — — — LNU360 64030.6 — — — 15.9 0.28 −1 — —— LNU343 64208.1 — — — 15.8 0.17 −2 11.6 0.19 −3 LNU343 64208.4 — — —15.9 0.28 −1 11.5 0.12 −4 LNU328 64150.1 206.9 0.20  7 14.5 0.10 −10 11.0 L −8 LNU328 64150.2 — — — — — — 11.3 0.04 −5 LNU328 64151.2 — — — —— — 11.2 0.03 −6 LNU322 63917.2 — — — 15.7 0.24 −2 — — — LNU317 64093.3— — — 15.9 0.28 −1 11.5 0.12 −4 LNU317 64097.2 — — — 15.5 0.13 −4 11.50.12 −4 LNU317 64097.3 215.6 0.07 12 — — — 11.5 0.12 −4 LNU306 64132.1 —— — 15.9 0.28 −1 — — — LNU306 64132.6 — — — — — — 11.2 0.03 −6 LNU30564111.3 — — — 15.6 0.04 −3 — — — LNU305 64114.1 237.5 0.11 23 — — — — —— LNU305 64115.1 — — — — — — 11.5 0.12 −4 CONT. — 192.5 — — 16.1 — —11.9 — — LNU499 64146.8 323.1 0.03 13 — — — — — — LNU499  64147.11 347.50.17 21 — — — — — — LNU485 63825.1 401.2 0.01 40 — — — — — — LNU48563827.1 307.5 0.17  7 — — — — — — LNU468 63492.2 315.6 0.12 10 — — — — —— LNU468 63493.4 337.5 0.08 18 — — — — — — LNU467 63715.1 327.9 0.12 14— — — — — — LNU462 63503.2 334.4 0.28 16 — — — — — — LNU450 63708.3449.4 0.13 56 — — — — — — LNU450 63708.6 463.8 0.01 62 — — — — — —LNU450 63709.4 456.2 0.06 59 — — — — — — LNU450 63710.2 444.4 0.03 55 —— — — — — LNU450 63712.3 478.8 0.06 67 — — — — — — LNU448 63706.5 475.60.04 66 — — — — — — LNU429 63937.3 423.8 L 48 — — — — — — LNU429 63937.4342.5 0.10 19 — — — — — — LNU429 63938.5 315.7 0.27 10 — — — — — —LNU416 64134.5 323.1 0.07 13 — — — — — — LNU399 63944.4 324.4 0.02 13 —— — — — — LNU399 63944.6 326.9 0.03 14 — — — — — — LNU395 64143.6 328.10.29 14 — — — — — — LNU395 64145.4 350.6 0.20 22 — — — — — — LNU39263698.2 325.0 0.09 13 — — — — — — LNU390 63539.2 321.9 0.10 12 — — — — —— LNU375 63452.2 316.4 0.18 10 — — — — — — LNU375 63454.2 358.1 0.03 25— — — — — — LNU349 63989.1 309.3 0.20  8 — — — — — — LNU347 63510.2348.8 0.27 21 — — — — — — LNU323 63420.1 308.1 0.29  7 — — — — — —LNU323 63421.2 336.9 0.13 17 — — — — — — LNU323 63424.4 324.6 0.26 13 —— — — — — CONT. — 287.1 — — — — — — — — Table 81: “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant. Thetransgenes were under the transcriptional regulation of the new At6669promoter (SEQ ID NO: 3829).

TABLE 82 Genes showing improved plant performance at Low N growthconditions under regulation of At6669 promoter Leaf Blade Area PlotCoverage [cm²] Leaf Number [cm²] Gene P- % P- % P- % Name Event # Ave.Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LNU519 64678.1 — — — — — —62.3 0.25 16 LNU519 64679.1 1.0 0.13  9 — — — — — — LNU519 64681.8 1.00.02 12 10.8 0.10 3 62.3 0.02 16 LNU503 64203.3 1.0 0.23  6 — — — 59.20.15 10 LNU460 64359.4 1.0 0.02 13 11.1 0.02 6 62.3 0.08 16 LNU46064362.1 — — — — — — 57.7 0.26  7 LNU459 64542.1 0.9 0.25  5 — — — 57.10.26  6 LNU421 64302.7 1.0 0.10 10 — — — — — — LNU412  63940.11 1.0 0.09 9 — — — — — — LNU409 64687.2 1.0 0.01 16 — — — 63.0 0.01 17 LNU40964688.2 1.0 0.03 17 — — — 62.1 0.02 15 LNU408 64249.4 1.0 0.20  6 — — —— — — LNU385 64245.3 1.0 0.20 11 10.7 0.25 2 61.3 0.10 14 LNU360 64029.21.0 0.24  6 — — — — — — LNU360 64030.1 1.1 0.02 22 — — — 69.1 L 28LNU336 64447.2 — — — 10.9 0.04 5 — — — LNU336 64447.4 — — — — — — 57.00.26  6 LNU331 64212.3 — — — — — — 57.6 0.19  7 LNU331 64215.3 1.0 0.06 9 — — — 62.2 0.05 15 LNU327 64490.3 1.0 0.05 15 — — — 63.7 0.02 18LNU290 64369.3 1.0 0.20  6 — — — — — — CONT. — 0.9 — — 10.5 — — 53.8 — —LNU502 64038.5 0.9 0.02 13 — — — 52.4 0.15  8 LNU498 64184.3 — — — 10.80.14 5 — — — LNU493 64191.3 0.9 0.05 10 11.4 0.17 11  57.1 0.01 17LNU485 63825.1 — — — 10.8 0.02 5 54.0 0.06 11 LNU456 63991.2 0.9 0.24  6— — — — — — LNU456 63991.8 0.9 0.20  9 11.4 0.27 11  55.3 0.08 14 LNU45564187.4 0.9 0.07 10 — — — 51.8 0.21  7 LNU455 64187.5 0.9 0.18 10 10.60.16 3 55.2 0.17 14 LNU371 63974.6 0.9 0.07 10 — — — 55.3 0.04 14 LNU34364208.4 — — — 11.1 0.23 8 — — — LNU328 64150.1 — — — 11.0 0.28 7 55.60.11 14 LNU328 64151.2 1.0 0.02 14 — — — 54.4 0.05 12 LNU322 63917.2 — —— 10.9 L 6 — — — LNU305 64115.1 0.9 0.27  7 — — — 52.5 0.14  8 CONT. —0.8 — — 10.3 — — 48.6 — — LNU468 63492.2 0.8 0.12  9 — — — — — — LNU46763714.4 0.8 0.29  5 — — — — — — LNU450 63708.6 0.8 0.01 16 — — — 44.90.15  8 LNU448 63705.4 0.8 0.13  8 — — — — — — LNU448 63706.5 0.8 0.2312 — — — — — — LNU375 63454.2 0.9 0.06 18 — — — 48.0 0.02 15 LNU32363421.2 0.8 0.26  6 — — — — — — CONT. — 0.7 — — — — — 41.6 — — Table 82.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant. The transgenes were under the transcriptionalregulation of the new At6669 promoter (SEQ ID NO: 3829).

TABLE 83 Genes showing improved plant performance at low Nitrogen growthconditions under regulation of At6669 promoter RGR Of RGR Of RGR OfRosette Leaf Number Plot Coverage Diameter Gene % % % Name Event # Ave.P-Val. Incr. Ave. P-Val. Incr. Ave. P-Val. Incr. LNU519 64678.1 — — —7.9 0.12 18 — — — LNU519 64681.8 — — — 8.0 0.10 19 0.4 0.12 16 LNU50364203.3 — — — 7.6 0.27 13 0.4 0.23 13 LNU460 64359.4 — — — 8.0 0.10 190.4 0.05 21 LNU460 64360.3 — — — — — — 0.4 0.22 12 LNU409 64687.2 — — —8.1 0.09 20 0.4 0.17 14 LNU409 64688.2 — — — 7.9 0.12 18 0.4 0.07 19LNU385 64245.3 — — — 7.6 0.25 13 0.4 0.30 11 LNU385 64246.6 — — — — — —0.4 0.25 12 LNU360 64029.2 — — — — — — 0.4 0.15 15 LNU360 64030.1 — — —8.9 L 32 0.4 0.03 23 LNU360 64030.4 — — — — — — 0.4 0.29 12 LNU33164212.3 — — — — — — 0.4 0.26 11 LNU331 64215.3 — — — 7.8 0.15 16 0.40.21 13 LNU327 64490.3 — — — 8.3 0.06 23 0.4 0.05 21 LNU290 64369.3 — —— — — — 0.4 0.12 17 CONT. — — — — 6.7 — — 0.3 — — LNU493 64191.3 0.80.10 19 6.9 0.07 15 — — — LNU493 64191.4 — — — 7.0 0.08 17 — — — LNU48563825.1 — — — 6.6 0.27  9 — — — LNU456 63991.8 0.8 0.03 27 6.9 0.09 14 —— — LNU455 64187.5 — — — 6.9 0.09 14 — — — LNU371 63974.6 0.8 0.25 146.9 0.10 14 0.4 0.21 10 LNU360 64030.4 — — — — — — 0.4 0.27  9 LNU34364208.4 0.8 0.09 20 — — — — — — LNU328 64150.1 0.8 0.26 15 6.8 0.11 13 —— — LNU328 64151.2 — — — 6.9 0.10 14 0.4 0.15 12 LNU306 64132.6 0.8 0.1816 — — — — — — CONT. — 0.7 — — 6.0 — — 0.3 — — LNU468 63492.2 — — — — —— 0.4 0.19 13 LNU462 63503.2 — — — — — — 0.4 0.26 11 LNU375 63454.2 — —— 6.3 0.24 17 0.4 0.10 15 LNU323 63421.2 — — — — — — 0.4 0.28 10 CONT. —— — — 5.4 — — 0.4 — — Table 83. “CONT.”—Control; “Ave.”—Average; “%Incr.” = % increment; “p-val.”—p-value; L means that p-value is lessthan 0.01 p < 0.1 was considered as significant. The transgenes wereunder the transcriptional regulation of the new At6669 promoter (SEQ IDNO: 3829).

TABLE 84 Genes showing improved plant performance at low Nitrogen growthconditions under regulation of At6669 promoter Rosette Area RosetteDiameter Harvest Index [cm²] [cm] Gene P- % P- % P- % Name Event # Ave.Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LNU519 64678.1 — — — 7.8 0.2516 — — — LNU519 64681.8 — — — 7.8 0.02 16 4.6 0.04 7 LNU503 64203.3 — —— 7.4 0.15 10 4.5 0.17 4 LNU460 64359.4 0.4 0.29  4 7.8 0.08 16 4.7 0.168 LNU460 64362.1 — — — 7.2 0.26  7 — — — LNU459 64542.1 0.4 0.15  7 7.10.26  6 4.5 0.12 5 LNU421 64303.4 — — — — — — 4.5 0.17 4 LNU409 64687.2— — — 7.9 0.01 17 4.8 0.01 10  LNU409 64688.2 — — — 7.8 0.02 15 4.8 0.1010  LNU385 64245.3 — — — 7.7 0.10 14 4.7 0.15 9 LNU360 64030.1 — — — 8.6L 28 4.9 L 14  LNU360 64030.4 0.4 0.10  9 — — — — — — LNU336 64447.4 — —— 7.1 0.26  6 — — — LNU336 64449.4 0.4 0.21  7 — — — — — — LNU33164212.3 — — — 7.2 0.19  7 4.6 0.10 6 LNU331 64214.2 — — — — — — 4.5 0.283 LNU331 64215.3 — — — 7.8 0.05 15 4.7 0.01 9 LNU327 64490.3 — — — 8.00.02 18 4.8 0.02 10  LNU290 64369.3 — — — — — — 4.5 0.22 4 CONT. — 0.4 —— 6.7 — — 4.3 — — LNU502 64038.5 — — — 6.6 0.15  8 4.5 0.05 7 LNU50264039.4 0.4 L  8 — — — — — — LNU500 64221.2 0.5 0.30 19 — — — — — —LNU500 64223.1 0.4 0.09  5 — — — — — — LNU493 64191.3 — — — 7.1 0.01 174.5 0.04 7 LNU485 63825.1 — — — 6.7 0.06 11 — — — LNU480 64018.4 0.40.29  9 — — — — — — LNU456 63991.8 — — — 6.9 0.08 14 4.4 0.21 7 LNU45663992.5 0.5 0.04 16 — — — — — — LNU455 64187.4 — — — 6.5 0.21  7 4.40.13 5 LNU455 64187.5 0.5 L 12 6.9 0.17 14 4.5 0.04 9 LNU455 64189.7 0.40.15  4 — — — — — — LNU371  63973.10 0.4 0.26  3 — — — — — — LNU37163974.6 — — — 6.9 0.04 14 4.5 0.04 7 LNU360 64030.1 0.4 0.13  4 — — — —— — LNU328 64150.1 — — — 7.0 0.11 14 4.5 0.11 7 LNU328 64151.2 0.4 0.17 4 6.8 0.05 12 4.5 0.06 8 LNU317 64097.2 0.4 0.14  9 — — — — — — LNU30664132.2 0.5 0.19 13 — — — — — — LNU305 64115.1 0.5 0.16 13 6.6 0.14  84.4 0.11 5 CONT. — 0.4 — — 6.1 — — 4.2 — — LNU499 64146.8 0.3 0.01 21 —— — — — — LNU485 63828.3 0.3 0.03 22 — — — — — — LNU468 63493.4 0.3 0.0122 — — — — — — LNU467 63718.1 0.3 0.05 14 — — — — — — LNU467 63718.2 0.30.07 17 — — — — — — LNU462 63502.2 0.3 0.09 19 — — — — — — LNU46263503.2 0.3 0.08 18 — — — — — — LNU450 63708.3 0.3 L 27 — — — — — —LNU450 63708.6 — — — 5.6 0.15  8 — — — LNU450 63710.2 0.3 0.06 27 — — —— — — LNU450 63712.3 0.3 0.24 25 — — — — — — LNU448 63705.2 0.3 0.23  9— — — — — — LNU448 63706.5 0.3 0.02 20 — — — — — — LNU448 63707.2 0.3 L32 — — — — — — LNU429 63937.4 0.3 0.22 17 — — — — — — LNU416  64134.110.3 0.19 18 — — — — — — LNU416 64134.2 0.3 0.15 11 — — — — — — LNU39963944.2 0.3 0.12 19 — — — — — — LNU395 64142.8 0.3 0.06 14 — — — — — —LNU395 64143.5 0.3 0.06 14 — — — — — — LNU392 63697.4 0.3 0.11 16 — — —— — — LNU390 63539.4 0.3 0.12 11 — — — — — — LNU375 63452.3 0.3 0.28 23— — — — — — LNU375 63454.2 0.3 L 31 6.0 0.02 15 4.4 0.01 11  LNU34963989.6 0.3 L 24 — — — — — — LNU349 63990.2 0.3 0.24 11 — — — — — —LNU347 63508.1 0.3 0.22 13 — — — — — — LNU347 63513.4 0.3 0.09 16 — — —— — — LNU329 63427.3 0.3 0.22 22 — — — — — — LNU329 63430.3 0.3 0.05 19— — — — — — LNU323 63421.2 0.3 0.29 19 — — — 4.1 0.16 5 CONT. — 0.3 — —5.2 — — 3.9 — — Table 84. “CONT.”—Control; “Ave.”—Average; “% Incr.” = %increment; “p-val.”—p-value; L means that p-value is less than 0.01, p <0.1 was considered as significant. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 3829).

TABLE 85 Genes showing improved plant performance at low Nitrogen growthconditions under regulation of At6669 promoter 1000 Seed Weight SeedYield [mg] [mg] Gene % % Name Event # Ave. P-Val. Incr. Ave. P-Val.Incr. LNU508 64460.1 94.2 0.17  6 — — — LNU421 64303.4 — — — 25.2 L 14 LNU409 64687.2 — — — 23.5 0.02 7 LNU408 64250.8 — — — 23.4 0.02 7 LNU38564245.3 97.2 0.05 10 — — — LNU348 64472.2 — — — 26.9 0.13 22  LNU34064292.5 94.1 0.19  6 — — — LNU336 64447.4 — — — 22.7 0.20 3 LNU33164212.1 — — — 24.3 L 10  LNU327 64491.2 101.1  0.06 14 — — — LNU31664567.1 — — — 26.1 L 19  LNU290 64369.3 — — — 23.9 0.29 9 CONT. — 88.5 —— 22.0 — — LNU502 64038.2 — — — 22.8 0.14 10  LNU502 64038.5 — — — 24.4L 18  LNU502 64039.3 — — — 22.0 0.17 6 LNU500 64222.1 — — — 24.9 0.0720  LNU498 64185.3 88.3 0.18 11 — — — LNU498 64186.1 87.7 0.14 11 — — —LNU498 64186.2 86.9 0.12 10 — — — LNU493 64191.3 — — — 22.7 0.09 9LNU485 63825.1 — — — 30.8 0.01 48  LNU485 63828.3 — — — 21.3 0.22 2LNU456 63991.2 86.6 0.13  9 — — — LNU456 63991.8 91.7 0.14 16 — — —LNU456 63992.6 — — — 21.3 0.16 3 LNU455 64189.4 — — — 21.3 0.22 2 LNU38464161.2 — — — 22.1 0.08 6 LNU371  63973.12 — — — 21.8 0.22 5 LNU37163974.4 — — — 22.4 0.03 8 LNU371 63974.6 — — — 23.6 L 14  LNU360 64029.890.6 0.04 14 — — — LNU360 64030.1 — — — 21.3 0.29 2 LNU343 64208.1 — — —23.6 0.26 14  LNU343 64208.2 — — — 24.7 L 19  LNU328 64151.1 — — — 23.40.12 13  LNU322 63918.4 — — — 21.8 0.03 5 LNU317 64097.1 — — — 21.3 0.223 LNU306 64130.7 — — — 21.5 0.24 3 LNU306 64132.6 — — — 24.4 0.07 17 CONT. — 79.2 — — 20.8 — — LNU499 64146.8 102.9  L 35 — — — LNU499 64147.11 98.8 0.19 30 — — — LNU485 63825.1 95.6 0.05 26 24.4 0.02 51 LNU485 63825.2 87.4 0.27 15 — — — LNU485 63828.3 — — — 16.9 0.26 5LNU468 63493.2 96.7 0.03 27 16.8 0.30 4 LNU468 63493.4 108.7  0.03 43 —— — LNU467 63714.4 — — — 17.2 0.24 6 LNU467 63718.1 — — — 17.2 0.05 7LNU462 63503.2 103.3  0.01 36 17.4 0.04 8 LNU462 63505.1 — — — 17.0 0.086 LNU450 63708.3 150.5  0.05 98 — — — LNU450 63708.6 131.6  L 73 22.5 L39  LNU450 63709.4 123.4  0.18 62 — — — LNU450 63710.2 148.3  0.07 95 —— — LNU450 63712.3 157.1  L 107  — — — LNU448 63705.3 — — — 16.8 0.17 4LNU448 63706.5 149.8  L 97 — — — LNU448 63707.2 91.0 0.15 20 18.3 L 13 LNU429 63937.3 — — — 20.1 0.04 24  LNU429 63937.4 105.1  L 38 — — —LNU429 63938.5 — — — 16.9 0.30 5 LNU416 64134.2 — — — 16.8 0.20 4 LNU39963944.2 90.8 0.08 19 — — — LNU399 63945.3 87.2 0.20 15 — — — LNU39564142.8 — — — 16.7 0.26 3 LNU395 64143.5 100.6  0.10 32 — — — LNU39564145.4 96.9 0.26 27 — — — LNU392 63701.2 — — — 21.1 L 31  LNU39063539.2 94.6 0.15 24 — — — LNU390 63539.3 — — — 17.5 0.02 8 LNU39063539.4 — — — 16.7 0.23 3 LNU375 63452.3 102.5  0.01 35 — — — LNU37563454.2 123.5  0.01 62 — — — LNU349 63989.6 93.9 0.05 23 16.7 0.30 4LNU349 63990.2 91.3 0.27 20 — — — LNU329 63428.2 — — — 18.6 0.01 16 LNU323 63421.2 106.4  0.26 40 — — — LNU323 63424.4 84.4 0.29 11 — — —CONT. — 76.1 — — 16.1 — — Table 85. “CONT.”—Control; “Ave.”—Average; “%Incr.” = % increment; “p-val.”—p-value; L means that p-value is lessthan 0.01, p < 0.1 was considered as significant. The transgenes wereunder the transcriptional regulation of the new At6669 promoter (SEQ IDNO: 3829).

TABLE 86 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Inflorescence Dry Weight[mg] Flowering Emergence Gene P- % P- % P- % Name Event # Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LNU503 64203.3 620.0 0.23 9 — — —— — — LNU460 64360.1 623.1 0.13 10 — — — — — — LNU421 64303.3 — — — 16.50.25 −1 — — — LNU421 64303.4 615.6 0.19 8 16.5 0.25 −1 — — — LNU33664449.4 — — — 15.9 L −4 11.9 0.07 −5 LNU290 64372.2 — — — 16.4 0.27 −1 —— — CONT. — 568.8 — — 16.6 — — 12.6 — — LNU502 64038.5 734.4 L 9 — — —11.1 0.07 −6 LNU502 64039.4 698.8 0.06 4 — — — — — — LNU500 64221.2693.8 0.10 3 — — — — — — LNU500 64221.6 — — — — — — 11.2 0.11 −5 LNU50064222.1 760.6 0.24 13 — — — — — — LNU498 64184.2 705.0 0.05 5 — — — — —— LNU498 64185.3 700.6 0.15 4 — — — — — — LNU498 64186.1 747.5 L 11 — —— — — — LNU493 64190.1 — — — — — — 11.2 0.11 −5 LNU493 64191.3 799.4 L19 — — — — — — LNU485 63825.1 878.8 0.18 31 — — — 11.4 0.27 −4 LNU45663991.2 — — — — — — 11.0 0.04 −7 LNU456 63991.8 — — — 16.1 0.28 −2 11.20.11 −5 LNU456 63992.5 — — — — — — 11.2 0.10 −6 LNU455 64187.4 — — — — —— 11.1 0.06 −7 LNU455 64189.4 712.5 0.20 6 — — — — — — LNU412  63940.10764.4 L 14 — — — — — — LNU384 64161.6 763.8 0.09 14 — — — — — — LNU37163974.6 — — — — — — 11.4 0.27 −4 LNU343 64208.1 706.2 0.20 5 — — — — — —LNU343 64208.4 731.2 L 9 — — — — — — LNU343 64209.2 748.8 0.15 11 — — —— — — LNU328 64150.1 693.8 0.22 3 — — — 11.0 0.04 −7 LNU328 64150.2 — —— — — — 11.3 0.16 −4 LNU328 64151.1 — — — — — — 11.1 0.06 −7 LNU32864151.2 — — — — — — 11.1 0.07 −6 LNU322 63918.3 687.5 0.26 2 — — — 11.40.27 −4 LNU317 64097.1 — — — 15.9 0.03 −3 11.0 0.04 −7 LNU317 64097.2 —— — — — — 11.3 0.28 −5 LNU317 64097.3 — — — — — — 11.4 0.27 −4 LNU30564111.1 — — — — — — 11.1 0.07 −6 LNU305 64115.1 768.8 0.05 14 — — — — —— CONT. — 672.5 — — 16.4 — — 11.9 — — LNU499  64146.12 — — — — — — 13.60.02 −14 LNU485 63825.2 — — — 16.7 0.26 −3 — — — LNU468 63493.2 — — — —— — 14.1 0.05 −12 LNU467 63716.1 — — — — — — 13.5 0.02 −15 LNU46763718.2 — — — — — — 13.8 0.02 −14 LNU462 63503.1 — — — — — — 13.5 0.02−15 LNU450 63708.3 996.2 0.28 22 — — — — — — LNU450 63712.3 940.0 0.1415 — — — — — — LNU448 63706.5 1195.0  0.06 46 — — — — — — LNU429 63937.4— — — — — — 13.8 0.02 −14 LNU416 64134.1 — — — — — — 13.9 0.06 −12LNU395 64145.1 — — — — — — 14.1 0.05 −12 LNU375 63452.2 — — — 16.2 0.10−6 — — — LNU349 63990.4 — — — — — — 13.8 0.02 −14 LNU347 63510.2 — — — —— — 13.5 0.02 −15 LNU329 63427.3 — — — 16.7 0.26 −3 13.5 0.02 −15 CONT.— 816.2 — — 17.3 — — 15.9 — — Table 86. “CONT.”—Control; “Ave.”—Average;“% Incr.” = % increment; “p-val.”—p-value; L means that p-value is lessthan 0.01, p < 0.1 was considered as significant. The transgenes wereunder the transcriptional regulation of the new At6669 promoter (SEQ IDNO: 3829).

TABLE 87 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Leaf Blade Area Leaf PlotCoverage [cm²] Number [cm²] Gene P- % P- % P- % Name Event # Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LNU519 64678.1 — — — 11.4 0.24 7 —— — LNU508 64457.2 — — — 11.2 0.03 6 — — — LNU503 64203.3 — — — 11.00.14 4 — — — LNU503 64204.2 — — — 11.1 0.22 5 — — — LNU460 64359.4 1.30.09 5 11.1 0.17 4 — — — LNU460 64362.1 1.4 0.14 12 11.6 L 10 90.0 0.0220 LNU421 64302.7 — — — 11.1 0.22 5 — — — LNU421 64303.3 — — — 10.9 0.213 — — — LNU385 64245.5 — — — 11.3 0.20 7 — — — LNU360 64030.1 — — — 11.80.02 11 — — — LNU348 64472.2 1.3 0.15 5 11.4 0.04 8 81.2 0.03  8 LNU34864474.2 1.4 0.23 15 11.1 0.07 5 85.5 0.12 14 LNU340  64290.11 — — — 11.20.11 6 — — — LNU336 64449.4 1.3 0.02 8 — — — — — — LNU331 64212.1 — — —11.4 0.02 7 82.5 0.15 10 LNU331 64215.1 1.3 0.21 10 — — — — — — LNU33164215.3 — — — 11.9 L 13 — — — LNU290 64369.3 — — — 11.3 0.20 7 — — —LNU290 64372.1 1.4 0.08 11 — — — 82.5 0.12 10 CONT. — 1.2 — — 10.6 — —74.9 — — LNU502 64038.5 1.3 0.23 10 11.3 L 6 79.3 0.22 12 LNU500 64221.6— — — 11.4 0.10 7 — — — LNU498 64186.1 1.3 0.18 9 11.4 L 7 82.1 0.10 16LNU493 64190.1 — — — 10.9 0.08 2 — — — LNU493 64191.3 — — — 11.4 0.03 780.8 0.11 14 LNU493 64191.4 1.3 0.18 9 11.2 0.17 5 80.0 0.16 13 LNU48563825.1 1.4 0.10 23 — — — 87.7 0.02 23 LNU480 64018.1 1.3 0.21 10 11.40.18 7 80.4 0.11 13 LNU455 64187.4 — — — 11.5 0.15 8 — — — LNU37163974.6 — — — 10.9 0.08 2 — — — LNU360 64029.8 — — — 11.4 0.22 7 — — —LNU343 64208.4 — — — 11.8 0.27 10 — — — LNU343 64209.2 — — — 11.1 0.08 4— — — LNU328 64150.1 — — — 10.8 0.25 1 — — — LNU328 64151.1 1.3 0.13 11— — — — — — LNU328 64151.2 — — — 11.1 0.29 4 — — — LNU322 63917.2 — — —11.4 L 7 — — — LNU317 64097.3 1.3 0.26 8 — — — 81.3 0.09 14 LNU30564111.1 — — — 11.4 L 7 79.8 0.13 12 LNU305 64111.3 — — — 11.1 0.02 4 — —— LNU305 64115.1 1.3 0.06 13 11.6 0.30 9 85.5 0.03 20 CONT. — 1.2 — —10.7 — — 71.1 — — LNU462 63505.1 0.9 0.28 12 — — — — — — LNU429 63938.5— — — 10.5 0.29 10 — — — LNU395 64143.5 0.9 0.16 9 10.2 0.15 7 — — —LNU375 63452.2 — — — 10.1 0.16 5 — — — LNU323 63424.4 0.9 0.19 9 — — — —— — CONT. — 0.8 — —  9.6 — — — — — Table 87. “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant. Thetransgenes were under the transcriptional regulation of the new At6669promoter (SEQ ID NO: 3829).

TABLE 88 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of RGR Of RGR Of LeafNumber Plot Coverage Rosette Diameter Gene P- % P- % P- % Name Event #Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LNU503 64204.2 0.9 0.2314 — — — — — — LNU460 64360.1 0.8 0.29 12 — — — — — — LNU460 64362.1 — —— 11.7 0.12 21 0.5 0.04 14 LNU421 64302.7 0.8 0.28 11 — — — — — — LNU41263940.10 — — — 11.3 0.24 17 — — — LNU408 64248.10 — — — — — — 0.5 0.23 8LNU360 64030.1 0.9 0.13 17 — — — — — — LNU360 64030.6 0.9 0.24 14 — — —— — — LNU348 64474.2 — — — 11.2 0.27 15 0.5 0.11 12 LNU340 64290.11 0.90.18 17 — — — — — — LNU340 64292.5 — — — 11.3 0.25 16 — — — LNU33664449.4 — — — — — — 0.5 0.05 16 LNU331 64215.3 0.9 0.17 15 — — — — — —LNU290 64369.4 — — — — — — 0.5 0.17 9 CONT. — 0.8 — — 9.7 — — 0.5 — —LNU500 64221.6 0.8 0.18 14 — — — — — — LNU498 64186.1 0.9 0.05 21 10.70.28 16 — — — LNU485 63825.1 — — — 11.3 0.15 22 — — — LNU480 64018.1 0.90.16 19 — — — — — — LNU456 63991.2 0.8 0.20 15 — — — — — — LNU45564187.4 0.9 0.04 22 — — — — — — LNU360 64029.8 0.9 0.06 20 — — — — — —LNU343 64208.4 0.9 0.05 24 — — — — — — LNU328 64151.2 0.8 0.23 13 — — —— — — LNU305 64111.1 0.8 0.28 12 — — — — — — LNU305 64115.1 0.9 0.17 1611.1 0.19 20 — — — CONT. — 0.7 — — 9.2 — — — — — LNU429 63938.5 — — — —— — 0.5 0.12 15 LNU395 64143.5 — — — — — — 0.5 0.09 15 CONT. — — — — — —— 0.4 — — Table 88. “CONT.”—Control; “Ave.”—Average; “% Incr.” = %increment; “p-val.”—p-value; L means that p-value is less than 0.01, p <0.1 was considered as significant. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 3829).

TABLE 89 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Rosette Area RosetteDiameter Harvest Index [cm²] [cm] Gene P- % P- % P- % Name Event # Ave.Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LNU508 64459.2 0.4 0.27 4 — —— — — — LNU503 64204.2 — — — — — — 5.4 0.17 2 LNU460 64360.3 0.4 0.24 6— — — — — — LNU460 64362.1 — — — 11.2 0.02 20 5.8 0.03 10 LNU459 64543.2— — — — — — 5.4 0.28 2 LNU408 64248.10 — — — — — — 5.4 0.09 3 LNU34864472.2 — — — 10.2 0.03 8 — — — LNU348 64474.2 — — — 10.7 0.12 14 5.60.07 7 LNU336 64449.4 — — — — — — 5.7 0.28 8 LNU331 64212.1 — — — 10.30.15 10 — — — LNU331 64215.1 — — — — — — 5.6 L 6 LNU290 64369.4 — — — —— — 5.5 0.06 5 LNU290 64372.1 — — — 10.3 0.12 10 5.6 0.27 6 CONT. — 0.3— — 9.4 — — 5.3 — — LNU502 64038.5 — — — 9.9 0.22 12 5.7 0.28 8 LNU50264039.4 0.5 0.04 9 — — — — — — LNU498 64185.3 0.5 0.16 8 — — — — — —LNU498 64186.1 — — — 10.3 0.10 16 — — — LNU493 64191.3 — — — 10.1 0.1114 — — — LNU493 64191.4 — — — 10.0 0.16 13 — — — LNU485 63825.1 — — —11.0 0.02 23 5.8 0.06 9 LNU480 64018.1 — — — 10.1 0.11 13 5.7 0.14 8LNU455 64187.4 0.5 0.29 14 — — — — — — LNU455 64189.4 0.5 0.26 4 — — — —— — LNU412 63940.8 0.5 0.02 10 — — — — — — LNU322 63918.4 0.5 0.03 9 — —— — — — LNU317 64097.3 — — — 10.2 0.09 14 5.7 0.09 8 LNU305 64111.1 — —— 10.0 0.13 12 — — — LNU305 64115.1 — — — 10.7 0.03 20 5.6 0.21 6 CONT.— 0.5 — — 8.9 — — 5.3 — — LNU485 63826.1 0.3 0.02 14 — — — — — — LNU48563828.3 0.3 0.22 11 — — — — — — LNU468 63492.2 0.3 0.11 25 — — — — — —LNU462 63502.2 0.3 0.23 11 — — — — — — LNU462 63503.2 0.3 0.09 24 — — —— — — LNU450 63712.3 0.3 0.03 19 — — — — — — LNU448 63705.2 0.3 0.15 7 —— — — — — LNU448 63705.3 0.3 0.05 11 — — — — — — LNU448 63707.2 0.3 0.119 — — — — — — LNU429 63938.5 0.3 0.19 16 — — — 4.7 0.14 10 LNU39963944.2 0.3 0.24 15 — — — — — — LNU395 64143.5 — — — — — — 4.5 0.09 7LNU392 63698.2 0.3 0.09 8 — — — — — — LNU390 63539.3 0.3 0.25 6 — — — —— — LNU375 63452.3 0.3 0.07 19 — — — — — — LNU375 63454.2 0.3 L 18 — — —— — — LNU349 63989.6 0.3 0.19 10 — — — — — — LNU347 63513.3 0.3 0.02 32— — — — — — LNU329 63427.3 0.3 L 31 — — — — — — LNU329 63429.1 0.3 0.089 — — — — — — LNU323 63421.2 0.3 0.03 13 — — — — — — CONT. — 0.3 — — — —— 4.2 — — Table 89. “CONT.”—Control; “Ave.”—Average; “% Incr.” = %increment; “p-val.”—p-value; L means that p-value is less than 0.01, p <0.1 was considered as significant. The transgenes were under thetranscriptional regulation of the new At6669 promoter (SEQ ID NO: 3829).

TABLE 90 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Seed Yield [mg] 1000 SeedWeight [mg] Gene % % Name Event # Ave. P-Val. Incr. Ave. P-Val. Incr.LNU460 64360.1 215.8 0.21 12 — — — LNU421 64303.4 204.8 0.21 6 — — —LNU421 64304.3 207.3 0.09 8 — — — LNU408 64248.10 — — — 23.0 0.20 8LNU348 64472.2 — — — 26.4 0.07 24 LNU340 64292.5 202.0 0.25 5 — — —LNU331 64212.1 — — — 23.4 0.15 10 LNU331 64215.3 202.6 0.25 5 — — —CONT. — 192.8 — — 21.3 — — LNU502 64038.2 — — — 22.0 0.03 7 LNU50264038.5 — — — 29.6 0.29 44 LNU502 64039.4 350.0 0.04 14 — — — LNU50064222.1 — — — 25.2 L 22 LNU500 64223.1 — — — 21.4 0.24 4 LNU498 64185.3346.1 0.02 12 21.2 0.29 3 LNU498 64186.1 366.9 0.11 19 22.3 0.06 8LNU493 64191.3 — — — 24.4 0.14 18 LNU485 63825.1 — — — 27.4 0.06 33LNU485 63828.3 — — — 22.2 0.03 7 LNU455 64189.4 340.6 0.03 11 — — —LNU412 63940.8 337.2 0.04 9 — — — LNU384 64161.6 — — — 22.6 0.22 10LNU371 63974.4 — — — 21.2 0.26 3 LNU343 64208.1 — — — 21.4 0.25 4 LNU32864151.1 — — — 24.3 L 18 LNU306 64130.7 — — — 22.3 0.19 8 LNU306 64132.6— — — 24.3 0.04 18 LNU305 64115.1 373.2 0.15 21 22.2 0.28 8 CONT. —308.1 — — 20.6 — — LNU499 64146.12 — — — 21.0 0.06 22 LNU499 64146.8219.5 0.26 8 19.2 0.11 11 LNU485 63825.1 — — — 23.0 0.15 33 LNU48563826.1 226.6 0.13 12 — — — LNU467 63716.1 — — — 19.7 0.11 14 LNU46763718.2 219.7 0.26 8 — — — LNU462 63503.1 220.5 0.28 9 — — — LNU45063708.3 246.0 0.03 21 — — — LNU450 63712.3 279.6 L 38 — — — LNU44863705.3 259.6 0.22 28 — — — LNU448 63706.5 305.0 0.04 50 18.3 0.18 6LNU429 63938.5 220.5 0.23 9 — — — LNU416 64134.1 235.0 0.18 16 — — —LNU416 64134.2 224.4 0.16 11 — — — LNU399 63944.2 238.6 0.15 18 — — —LNU399 63944.6 228.5 0.28 13 — — — LNU395 64145.1 — — — 21.4 0.20 23LNU392 63701.2 — — — 20.7 0.12 20 LNU347 63513.3 237.3 0.05 17 — — —LNU329 63427.3 239.9 0.04 18 — — — CONT. — 202.7 — — 17.3 — — Table 90.“CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant. The transgenes were under the transcriptionalregulation of the new At6669 promoter (SEQ ID NO: 3829).

Example 18 Evaluation of Transgenic Arabidopsis NUE, Yield and PlantGrowth Rate Under Low or Normal Nitrogen Fertilization in GreenhouseAssay

Assay 2: Nitrogen Use efficiency measured until bolting stage: plantbiomass and plant growth rate at limited and optimal nitrogenconcentration under greenhouse conditions—This assay follows the plantbiomass formation and the rosette area growth of plants grown in thegreenhouse at limiting and non-limiting nitrogen growth conditions.Transgenic Arabidopsis seeds were sown in agar media supplemented with ½MS medium and a selection agent (Kanamycin). The T₂ transgenic seedlingswere then transplanted to 1.7 trays filled with peat and perlite in a1:1 ratio. The trays were irrigated with a solution containing nitrogenlimiting conditions, which were achieved by irrigating the plants with asolution containing 1.5 mM inorganic nitrogen in the form of KNO₃,supplemented with 1 mM KH₂PO₄, 1 mM MgSO₄, 3.6 mM KCl, 2 mM CaCl₂ andmicroelements, while normal nitrogen levels were achieved by applying asolution of 6 mM inorganic nitrogen also in the form of KNO₃ with 1 mMKH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂ and microelements. All plants were grownin the greenhouse until bolting. Plant biomass (the above ground tissue)was weighted in directly after harvesting the rosette (plant freshweight [FW]). Following plants were dried in an oven at 50° C. for 48hours and weighted (plant dry weight [DW]).

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying theAT6669 promoter and the selectable marker was used as control.

The plants were analyzed for their overall size, growth rate, freshweight and dry matter. Transgenic plants performance was compared tocontrol plants grown in parallel under the same conditions.Mock-transgenic plants expressing the uidA reporter gene (GUS-Intron) orwith no gene at all, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital imaging—A laboratory image acquisition system, which consists ofa digital reflex camera (Canon EOS 300D) attached with a 55 mm focallength lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which includes 4 light units (4×150 Watts light bulb) wasused for capturing images of plant samples.

The image capturing process was repeated every 2 days starting from day1 after transplanting till day 15. Same camera, placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. The tubs were squareshape include 1.7 liter trays. During the capture process, the tubeswere placed beneath the iron mount, while avoiding direct sun light andcasting of shadows.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat Hypertext Transfer Protocol://rsbweb (dot) nih (dot) gov/]. Imageswere captured in resolution of 10 Mega Pixels (3888×2592 pixels) andstored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, analyzed data was saved to text files andprocessed using the JMP statistical analysis software (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated,including leaf number, rosette area, rosette diameter, leaf blade area.

Vegetative growth rate: the relative growth rate (RGR) of leaf number(Formula XII, described above), rosette area (Formula V described above)and plot coverage (Formula XIX, described above) are calculated usingthe indicated formulas.

Plant Fresh and Dry weight—On about day 80 from sowing, the plants wereharvested and directly weight for the determination of the plant freshweight (FW) and left to dry at 50° C. in a drying chamber for about 48hours before weighting to determine plant dry weight (DW).

Statistical analyses—To identify genes conferring significantly improvedtolerance to abiotic stresses, the results obtained from the transgenicplants were compared to those obtained from control plants. To identifyoutperforming genes and constructs, results from the independenttransformation events tested were analyzed separately. Data was analyzedusing Student's t-test and results are considered significant if the pvalue was less than 0.1. The JMP statistics software package was used(Version 5.2.1, SAS Institute Inc., Cary, N.C., USA).

Experimental Results:

The genes listed in the following Tables were cloned under theregulation of a constitutive (At6669). The evaluation of each gene wasperformed by testing the performance of different number of events.Event with p-value<0.1 was considered statistically significant.

The genes listed in Tables 91-92 improved plant NUE when grown atlimiting nitrogen concentration levels. These genes produced largerplants with a larger photosynthetic area, biomass (fresh weight, dryweight, leaf number, rosette diameter, rosette area and plot coverage)when grown under limiting nitrogen conditions.

TABLE 91 Genes showing improved plant biomass production at limitingnitrogen growth conditions Dry Weight Fresh Weight Leaf [mg] [mg] NumberGene P- % P- % P- % Name Event # Ave. Val. Incr. Ave. Val. Incr. Ave.Val. Incr. LNU507 64087.1 — — — — — — 9.6 0.17 4 LNU507 64584.2 — — —237.5 0.12 11 — — — LNU479 65497.2 30.6 0.06 35 268.8 L 25 — — — LNU47965499.1 — — — 262.5 0.07 22 9.7 0.09 5 LNU423 64102.1 25.0 0.19 10 — — —— — — LNU418 65025.1 — — — — — — 9.8 0.09 6 LNU418 65027.2 — — — — — —9.8 0.22 6 LNU418 65028.2 — — — — — — 9.8 0.09 6 LNU401 65493.2 — — —231.2 0.27 8 — — — LNU377 64603.2 — — — 231.2 0.27 8 — — — LNU34463520.4 28.1 0.02 24 — — — 9.5 0.27 3 LNU337 64952.1 27.5 0.26 21 262.50.01 22 — — — LNU333 65295.1 — — — — — — 9.8 0.04 6 LNU333 65297.1 25.00.19 10 — — — — — — LNU304 64573.1 25.4 0.14 12 — — — — — — CONT. — 22.7— — 214.6 — — 9.2 — — LNU494 65302.1 25.6 0.12 28 206.2 0.18 18 — — —LNU479 65499.1 25.0 0.08 25 206.2 0.18 18 12.2 0.15 5 LNU423 64596.1 — —— — — — 13.2 0.05 14 LNU423 64598.3 24.4 0.19 22 — — — 12.0 0.29 3LNU418 65024.2 31.9 0.09 59 275.0 0.07 57 — — — LNU388 65487.1 26.2 0.1631 206.2 0.18 18 — — — LNU388 65487.2 — — — — — — 12.2 0.28 5 LNU37764604.3 — — — — — — 12.9 0.01 11 LNU339 65056.1 — — — — — — 12.8 0.06 10LNU339 65058.2 — — — — — — 12.2 0.19 6 LNU337 64955.2 23.8 0.17 19 — — —— — — LNU333 65295.1 24.4 0.19 22 — — — — — — LNU333 65295.2 22.5 0.2712 — — — — — — LNU333 65297.1 — — — — — — 13.1 0.13 13 LNU324 64233.7 —— — — — — 12.4 0.08 7 LNU292 64085.2 23.8 0.17 19 225.0 0.08 29 — — —CONT. — 20.0 — — 175.0 — — 11.6 — — LNU519 64679.1 — — — 264.3 0.16 11 —— — LNU508 64457.2 — — — 256.2 0.30 8 — — — LNU469 64308.4 — — — 300.00.03 26 — — — LNU469 64311.5 — — — — — — 9.8 0.09 4 LNU460 64359.3 — — —287.5 0.06 21 — — — LNU460 64360.1 45.6 0.04 26 — — — — — — LNU46064361.4 45.0 0.08 24 306.2 0.23 29 — — — LNU459 64541.4 48.1 0.24 33300.0 L 26 — — — LNU459 64542.4 — — — — — — 10.1 0.08 8 LNU442 64056.1 —— — 300.0 0.18 26 — — — LNU442 64057.1 48.8 0.18 34 — — — — — — LNU43964615.2 — — — 267.0 0.11 12 — — — LNU439 64615.4 40.0 0.27 10 — — — — —— LNU439 64616.2 — — — 262.5 0.27 11 — — — LNU439 64618.3 40.6 0.18 12287.5 0.24 21 — — — LNU421 64303.3 — — — 275.0 0.12 16 9.8 0.09 4 LNU42164303.4 — — — — — — 9.9 0.05 6 LNU421 64304.4 — — — 293.8 0.12 24 10.2 L10 LNU420 64006.3 46.2 0.15 28 287.5 0.24 21 — — — LNU420 64009.1 — — —295.5 0.29 24 — — — LNU409 64688.2 — — — 267.9 0.30 13 — — — LNU40964689.3 46.4 0.13 28 — — — — — — LNU408 64248.16 — — — 281.2 0.18 18 — —— LNU408 64249.4 — — — — — — 9.6 0.19 3 LNU368 64003.1 45.0 0.08 24 — —— — — — LNU368 64004.2 — — — 262.5 0.16 11 — — — LNU368 64004.3 — — — —— — 9.6 0.18 2 LNU363 64410.1 — — — 293.8 0.01 24 10.3 0.15 10 LNU36364411.2 — — — 306.2 L 29 — — — LNU363 64413.2 53.1 L 47 293.8 0.12 249.6 0.19 3 LNU331 64214.2 — — — 275.0 0.12 16 — — — LNU331 64215.1 — — —256.2 0.30 8 — — — LNU331 64215.3 45.0 0.03 24 312.5 L 32 10.0 0.17 7LNU316 64564.5 40.6 0.18 12 — — — — — — LNU314 64434.2 41.9 0.10 16 — —— 9.9 0.11 6 LNU290 64369.6 — — — 262.5 0.27 11 — — — CONT. — 36.2 — —237.5 — — 9.3 — — LNU509 64692.3 — — — — — — 9.5 0.20 3 LNU509 64692.6 —— — — — — 9.5 0.20 3 LNU504 64453.2 — — — — — — 9.7 0.15 5 LNU50164197.1 — — — — — — 9.6 0.24 4 LNU397 64376.4 — — — — — — 9.5 0.20 3LNU396 64315.15 84.4 0.25 81 — — — — — — LNU396 64317.4 — — — — — — 9.60.24 4 CONT. — 46.7 — — — — — 9.2 — — LNU513 63458.2 106.3 0.06 24 963.40.11 19 — — — LNU513 63459.2 99.4 0.02 16 868.8 0.30 7 9.6 0.07 4 LNU51263468.3 106.8 L 25 — — — — — — LNU512 63468.4 — — — — — — 9.4 0.19 3LNU512 63470.1 98.8 0.08 16 — — — — — — LNU512 63471.3 100.6 0.01 18893.8 0.21 10 9.6 0.23 4 LNU512 63471.4 93.8 0.13 10 — — — 9.7 0.03 5LNU451 63496.2 — — — — — — 9.4 0.29 2 LNU451 63497.5 — — — — — — 9.50.19 3 LNU451 63499.1 98.1 0.12 15 — — — — — — LNU451 63499.5 120.0 L 401018.8 L 25 — — — LNU451 63500.1 106.9 0.04 25 — — — — — — LNU42463474.3 97.5 0.25 14 868.8 0.30 7 — — — LNU424 63476.3 — — — — — — 9.50.19 3 LNU424 63477.2 — — — — — — 9.7 0.15 5 LNU415 63692.1 — — — — — —9.6 0.09 5 LNU415 63692.3 94.3 0.13 10 — — — — — — LNU411 63514.3 108.8L 27 987.5 0.01 22 — — — LNU411 63515.3 103.8 L 21 881.2 0.22 8 — — —LNU411 63518.1 — — — 956.2 0.03 18 — — — LNU375 63451.3 111.9 0.06 311000.0 0.07 23 — — — LNU375 63452.2 114.6 L 34 971.4 0.03 20 — — —LNU375 63454.1 103.1 0.02 21 943.8 0.04 16 9.6 0.23 4 LNU375 63454.2113.8 L 33 1050.0 L 29 — — — LNU370 63544.3 105.0 L 23 937.5 0.16 15 — —— LNU370 63545.6 107.5 0.20 26 1006.3 L 24 — — — LNU370 63548.2 101.4 L19 912.5 0.10 12 — — — LNU357 63533.1 97.5 0.03 14 — — — — — — LNU35763533.8 109.4 L 28 900.0 0.16 11 9.8 0.09 7 LNU357 63534.1 105.6 0.19 24943.8 0.18 16 9.5 0.19 3 LNU356 63444.1 97.5 0.03 14 — — — — — — LNU35663444.2 96.9 0.03 13 — — — — — — LNU356 63445.1 104.4 0.02 22 — — — — —— LNU351 63462.3 107.5 0.24 26 943.8 0.04 16 — — — LNU351 63463.2 105.00.03 23 868.8 0.30 7 — — — LNU351 63464.1 — — — — — — 9.8 0.04 6 LNU35163466.1 — — — — — — 9.6 0.07 4 LNU344 63520.2 93.1 0.28 9 — — — — — —LNU344 63521.1 98.1 0.12 15 — — — — — — LNU330 63438.1 104.4 0.10 22 — —— — — — LNU330 63440.2 98.1 0.02 15 — — — — — — LNU330 63441.1 — — — — —— 9.6 0.07 4 LNU326 63435.1 105.6 0.01 24 987.5 0.04 22 9.7 0.03 5LNU326 63436.1 99.4 0.17 16 893.8 0.15 10 — — — LNU319 63527.1 99.4 0.3016 893.8 0.21 10 — — — LNU319 63527.2 — — — — — — 9.5 0.10 3 LNU31963530.3 118.7 0.15 39 998.2 0.17 23 — — — LNU308 63414.1 100.6 0.08 18 —— — — — — LNU308 63414.4 106.9 0.22 25 943.8 0.04 16 — — — LNU30863417.5 115.6 0.15 35 — — — 9.7 0.03 5 LNU308 63417.8 — — — 977.7 0.1620 — — — LNU302 63378.3 108.1 0.07 27 968.8 0.13 19 — — — LNU302 63380.1118.8 0.12 39 1045.5 L 29 — — — LNU302 63381.1 111.9 L 31 1000.0 0.03 23— — — LNU302 63382.2 111.9 L 31 931.2 0.06 15 9.5 0.10 3 LNU291 63385.2101.9 0.03 19 975.0 0.05 20 9.7 0.03 5 LNU291 63387.1 93.8 0.09 10 — — —10.1 L 10 LNU291 63387.3 — — — 943.8 0.18 16 9.8 0.04 6 LNU291 63388.1118.8 L 39 1062.5 L 31 9.6 0.23 4 CONT. — 85.4 — — 812.5 — — 9.2 — —LNU482 64165.5 — — — — — — 9.8 0.12 4 LNU469 64313.9 41.9 0.17 12 350.00.23 9 — — — LNU444 64182.3 — — — 368.8 0.16 15 10.1 0.03 7 LNU44464183.1 — — — 350.0 0.23 9 — — — LNU442 64056.1 — — — 375.0 0.05 17 — —— LNU442 64553.1 — — — — — — 10.2 0.06 8 LNU442 64555.1 — — — — — — 9.90.05 5 LNU430 63935.1 43.8 0.03 17 375.0 0.21 17 — — — LNU430 63935.4 —— — — — — 9.9 0.11 5 LNU430 63936.2 — — — — — — 10.0 0.03 6 LNU39163979.3 — — — — — — 9.8 0.12 4 LNU391 63980.3 43.1 0.27 16 356.2 0.10 1110.2 0.10 9 LNU376 63986.8 — — — — — — 9.9 0.11 5 LNU366 64028.3 — — —381.2 0.02 19 10.4 0.12 11 LNU314 64433.3 — — — 381.2 0.10 19 — — —LNU314 64437.2 — — — 368.8 0.04 15 9.9 0.05 5 CONT. — 37.3 — — 320.8 — —9.4 — — LNU511 65037.1 — — — — — — 11.4 0.12 11 LNU492 64176.4 — — —187.5 0.15 8 11.4 0.12 11 LNU463 64283.4 — — — 237.5 0.05 37 12.2 0.0318 LNU463 64283.5 — — — 200.0 0.22 15 — — — LNU454 64796.2 — — — — — —11.1 0.27 7 LNU454 64796.3 — — — 193.8 0.11 11 11.2 0.20 9 LNU45464799.2 — — — — — — 11.8 0.14 14 LNU454 64800.5 — — — — — — 11.1 0.27 7LNU413 65022.4 25.6 0.24 62 — — — 11.4 0.14 10 LNU410 64971.2 — — —187.5 0.15 8 — — — LNU387 64808.1 — — — — — — 11.4 0.15 11 LNU37364826.4 21.2 0.21 34 — — — — — — LNU373 64830.1 23.1 L 46 — — — — — —LNU361 64835.2 — — — 193.8 0.11 11 — — — LNU355 65012.1 — — — — — — 11.10.28 7 LNU355 65015.2 — — — 218.8 L 26 12.1 0.03 17 LNU332 64823.2 — — —— — — 11.6 0.09 12 LNU307 64958.2 — — — 187.5 0.15 8 — — — LNU30764962.2 18.6 0.27 18 199.1 0.26 15 — — — LNU300 65030.2 — — — — — — 11.10.24 8 LNU300 65033.3 20.0 0.30 27 — — — — — — CONT. — 15.8 — — 173.8 —— 10.3 — — LNU517 64296.3 40.0 0.23 19 300.0 0.25 19 10.1 L 10 LNU51764297.9 — — — 287.5 0.12 14 — — — LNU514 64364.2 — — — 312.5 0.04 24 — —— LNU514 64365.3 — — — 325.0 0.02 29 10.1 0.17 9 LNU514 64366.1 — — —331.2 0.05 31 — — — LNU509 64690.3 — — — 300.0 0.09 19 9.9 0.03 7 LNU50964690.6 — — — — — — 9.6 0.08 4 LNU509 64692.3 47.5 0.04 41 — — — — — —LNU509 64692.6 — — — 300.0 0.09 19 — — — LNU501 64197.1 45.6 0.18 35300.0 0.13 19 9.6 0.15 5 LNU501 64197.3 — — — 325.0 0.02 29 — — — LNU50164723.1 41.9 0.28 24 — — — — — — LNU461 64666.1 43.1 0.10 28 — — — — — —LNU397 64375.1 50.6 0.18 50 312.5 0.04 24 — — — LNU396 64315.13 — — — —— — 9.4 0.22 2 LNU396 64315.16 — — — 300.0 0.25 19 — — — LNU396 64317.3— — — 331.2 0.29 31 — — — LNU396 64317.4 — — — — — — 9.8 0.22 6 LNU38664394.3 — — — — — — 9.6 0.26 4 LNU381 64285.5 — — — — — — 9.9 0.30 7LNU372 64483.3 — — — 306.2 0.04 21 9.7 0.16 5 LNU369 64386.1 43.8 0.0830 293.8 0.08 17 — — — LNU369 64387.1 42.5 0.21 26 306.2 0.12 21 — — —LNU369 64387.2 — — — 325.0 0.02 29 10.1 L 9 LNU369 64389.2 — — — 300.00.09 19 9.8 0.05 6 LNU365 64708.1 — — — 305.4 0.14 21 — — — LNU36564711.3 — — — — — — 10.1 0.30 10 LNU350 64674.4 — — — 337.5 0.21 34 — —— LNU350 64676.2 — — — 281.2 0.20 12 — — — LNU350 64677.2 40.0 0.27 19306.2 0.29 21 — — — LNU342 64035.3 — — — 306.2 0.12 21 — — — LNU34264035.8 40.0 0.23 19 312.5 0.04 24 — — — LNU313 64661.8 — — — 331.2 0.0531 — — — LNU313 64663.2 — — — — — — 9.9 L 7 LNU313 64664.1 46.2 0.05 37325.0 0.02 29 — — — LNU313 64664.3 — — — 281.2 0.20 12 — — — LNU29464658.7 40.6 0.27 20 — — — — — — CONT. — 33.8 — — 252.1 — — 9.2 — —LNU472 63949.8 — — — — — — 9.6 0.26 4 LNU458 63893.1 33.8 0.13 46 — — —— — — LNU425 63911.12 — — — — — — 9.4 0.07 3 LNU419 63896.2 — — — 250.00.27 7 9.5 0.17 3 LNU403 64237.1 — — — 268.8 0.04 15 — — — LNU39363978.6 — — — 262.5 0.06 12 — — — LNU374 63997.2 — — — — — — 9.6 0.01 4LNU371 63974.4 — — — — — — 9.7 0.18 5 LNU335 64169.2 — — — 262.5 0.20 12— — — CONT. — 23.1 — — 234.4 — — 9.2 — — LNU520 64157.4 — — — — — — 9.20.25 3 LNU518 64014.5 — — — — — — 9.2 0.29 3 LNU518 64016.4 — — — — — —9.5 0.20 6 LNU500 64221.6 38.1 0.02 29 — — — 9.7 0.06 8 LNU496 64195.1 —— — — — — 9.9 0.12 11 LNU493 64190.1 — — — — — — 9.3 0.25 4 LNU49364191.3 — — — — — — 9.6 0.03 8 LNU481 64140.1 — — — — — — 9.6 0.09 7LNU472 63919.5 — — — — — — 9.2 0.29 3 LNU472 63949.7 35.6 0.06 20 — — —— — — LNU458 63894.3 — — — — — — 9.8 0.04 10 LNU458 63895.1 — — — — — —9.4 0.06 6 LNU419 63896.2 46.2 0.07 56 — — — 9.4 0.28 5 LNU419 63897.633.2 0.29 12 — — — — — — LNU405 64159.8 38.8 0.07 31 — — — — — — LNU34364208.2 35.9 0.08 21 — — — — — — LNU340 64290.7 — — — 237.5 0.18 10 — —— LNU328 64150.4 39.4 0.30 33 237.5 0.18 10 — — — LNU327 64491.2 45.0 L52 268.8 0.01 24 — — — LNU322 63917.2 38.8 0.28 31 — — — — — — LNU32263918.1 43.1 L 46 — — — — — — LNU322 63918.3 — — — — — — 9.3 0.25 4LNU306 64132.1 — — — — — — 9.3 0.13 4 LNU305 64111.1 41.9 0.16 42 — — —9.2 0.19 3 LNU305 64111.3 — — — 237.5 0.18 10 9.6 0.27 8 LNU305 64114.1— — — — — — 9.2 0.29 3 CONT. — 29.6 — — 216.1 — — 8.9 — — LNU503 64203.383.1 0.23 9 606.2 0.23 7 — — — LNU503 64203.5 — — — 781.2 0.25 38 — — —LNU502 64038.5 — — — — — — 10.9 0.21 5 LNU502 64040.4 108.8 0.29 42756.2 0.09 33 — — — LNU482 64164.8 — — — 637.5 0.13 12 — — — LNU48064018.3 103.8 0.19 36 — — — — — — LNU444 64181.1 — — — — — — 11.0 0.26 7LNU444 64181.2 108.1 L 41 693.8 L 22 10.8 0.20 5 LNU444 64182.3 86.90.09 14 — — — — — — LNU430 63934.3 83.1 0.23 9 662.5 0.18 17 — — —LNU430 63935.1 90.6 L 19 656.2 0.25 16 — — — LNU430 63936.1 94.4 L 23 —— — — — — LNU430 63952.1 115.6 0.25 51 806.2 L 42 — — — LNU403 64236.3 —— — 606.2 0.23 7 — — — LNU403 64237.1 — — — 681.2 0.09 20 — — — LNU40364239.1 86.9 0.09 14 643.8 0.29 14 — — — LNU393 63976.2 90.0 0.29 18637.5 0.13 12 — — — LNU393 63977.3 98.1 0.04 28 706.2 0.07 25 — — —LNU393 63977.6 96.2 0.27 26 693.8 0.08 22 — — — LNU391 63980.6 80.6 0.285 — — — — — — LNU385 64246.3 — — — 797.3 0.06 41 — — — LNU376 63985.193.1 0.08 22 681.2 0.09 20 — — — LNU376 63987.2 101.9 L 33 712.5 0.10 26— — — LNU374 63997.2 96.9 0.05 27 700.0 L 24 — — — LNU374 63997.4 86.20.04 13 687.5 L 21 — — — LNU366 64027.2 91.9 0.16 20 — — — — — — LNU36664027.7 — — — 643.8 0.18 14 — — — LNU366 64028.3 107.5 0.22 41 762.50.01 35 — — — LNU353 64033.2 91.2 L 19 656.2 0.04 16 — — — LNU35364033.3 91.2 0.26 19 687.5 0.04 21 — — — LNU352 64199.1 — — — 700.0 0.0324 — — — LNU352 64200.10 128.1 0.06 68 850.0 L 50 11.2 0.05 8 LNU35264201.1 91.2 L 19 681.2 L 20 — — — LNU335 64168.18 98.1 0.23 28 756.20.15 33 10.7 0.25 4 LNU335 64168.19 150.0 L 96 950.0 L 68 10.9 0.12 5LNU317 64094.1 82.5 0.14 8 — — — — — — LNU317 64097.2 — — — 593.8 0.26 5— — — CONT. — 76.5 — — 566.7 — — 10.3 — — LNU499 64146.12 133.8 0.25 28968.8 0.05 14 — — — LNU499 64146.8 — — — 925.0 0.19 9 — — — LNU46863492.2 — — — 906.2 0.29 6 11.5 0.28 4 LNU467 63718.2 116.2 0.28 11 — —— — — — LNU462 63502.2 — — — — — — 12.2 0.16 11 LNU462 63505.1 — — — — —— 11.3 0.15 2 LNU455 64187.4 — — — — — — 11.7 0.10 6 LNU450 63708.6126.9 0.12 21 — — — — — — LNU450 63709.4 151.2 0.09 44 968.8 0.30 14 — —— LNU450 63710.2 — — — — — — 11.6 0.04 5 LNU448 63706.5 130.0 0.16 24993.8 0.23 17 — — — LNU429 63937.4 129.4 0.07 24 962.5 0.28 13 — — —LNU402 63914.2 — — — — — — 11.8 L 7 LNU399 63944.6 141.9 0.11 35 — — — —— — LNU395 64142.8 124.4 0.13 19 1018.8 0.03 20 — — — LNU392 63696.2141.9 0.01 35 962.5 0.05 13 — — — LNU392 63698.2 135.6 0.10 29 — — — — —— LNU392 63700.3 148.1 0.20 41 — — — 11.2 0.23 2 LNU349 63989.1 125.00.09 19 — — — — — — LNU349 63989.5 — — — 937.5 0.21 10 — — — LNU34763508.1 — — — — — — 11.6 0.02 5 LNU347 63510.4 — — — 925.0 0.27 9 11.60.21 5 LNU347 63513.3 122.5 0.15 17 987.5 0.03 16 — — — LNU329 63429.1178.1 0.03 70 1112.5 0.01 30 11.9 0.17 8 LNU323 63421.4 131.9 0.04 261043.8 L 22 11.4 0.23 4 LNU323 63424.1 150.6 0.02 44 — — — — — — CONT. —104.8 — — 852.5 — — 11.1 — — LNU511 65038.1 30.6 0.08 28 — — — — — —LNU492 64174.1 — — — 250.0 0.01 19 — — — LNU471 64838.1 — — — 231.2 0.1410 9.8 0.09 8 LNU413 65022.4 — — — 243.8 0.04 16 — — — LNU410 64971.128.1 0.13 17 237.5 0.05 13 — — — LNU410 64973.2 — — — — — — 9.4 0.08 3LNU387 64810.4 — — — 225.0 0.22 7 9.3 0.25 2 LNU382 64429.3 — — — 231.20.14 10 9.6 0.06 5 LNU373 64828.1 — — — — — — 9.6 0.02 5 LNU373 64830.134.4 0.02 43 256.2 0.01 22 — — — LNU355 65013.2 — — — — — — 9.5 0.04 4LNU307 64958.2 — — — 231.2 0.14 10 9.4 0.08 3 LNU307 64959.2 — — — 243.80.28 16 — — — LNU303 65043.1 — — — 237.5 0.05 13 9.6 0.25 5 LNU30365046.3 — — — — — — 9.9 0.29 9 LNU300 65032.1 30.6 0.03 28 — — — — — —CONT. — 24.0 — — 210.4 — — 9.1 — — LNU512 63468.3 87.5 0.20 21 — — — — —— LNU451 63499.1 82.5 0.24 14 881.2 0.08 14 — — — LNU424 63476.3 92.50.08 28 981.2 0.01 27 — — — LNU424 63478.1 — — — 818.8 0.28 6 — — —LNU424 63478.3 84.4 0.23 16 — — — — — — LNU415 63691.2 83.8 0.02 16850.0 0.08 10 — — — LNU415 63692.2 77.5 0.29 7 — — — — — — LNU41163514.3 78.8 0.20 9 — — — — — — LNU411 63517.1 86.9 0.30 20 — — — — — —LNU375 63451.3 — — — 812.5 0.30 5 — — — LNU375 63452.2 88.1 0.15 22943.8 0.07 22 — — — LNU375 63452.3 78.1 0.16 8 — — — — — — LNU37563454.2 — — — 856.2 0.15 11 — — — LNU370 63544.3 88.1 L 22 962.5 L 25 —— — LNU370 63545.2 86.2 0.02 19 968.8 0.10 26 — — — LNU357 63532.3 80.00.08 10 843.8 0.10 9 — — — LNU357 63533.1 80.0 0.07 10 818.8 0.28 6 12.10.05 12 LNU357 63533.8 81.9 0.05 13 837.5 0.12 9 — — — LNU356 63444.177.5 0.19 7 — — — — — — LNU356 63444.3 83.8 0.28 16 — — — — — — LNU35663445.1 82.5 0.03 14 856.2 0.15 11 — — — LNU351 63464.1 89.4 0.30 23 — —— — — — LNU344 63520.4 80.0 0.14 10 — — — — — — LNU330 63438.1 85.0 0.0117 — — — — — — LNU330 63441.2 89.4 0.30 23 — — — — — — LNU319 63527.188.8 L 22 912.5 0.01 18 — — — LNU319 63527.2 83.8 0.05 16 — — — — — —LNU319 63528.1 84.4 0.02 16 843.8 0.10 9 11.4 0.11 6 LNU319 63530.3 81.90.05 13 918.8 0.04 19 — — — LNU308 63417.5 — — — 875.0 0.06 14 — — —LNU302 63380.1 80.0 0.14 10 887.5 0.04 15 — — — LNU302 63382.2 84.4 0.3016 — — — — — — LNU291 63387.1 79.4 0.21 9 — — — — — — LNU291 63387.3 — —— 825.0 0.21 7 — — — LNU291 63388.1 78.1 0.16 8 856.2 0.08 11 — — —CONT. — 72.5 — — 770.8 — — 10.7 — — Table 91. “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant.

TABLE 92 Genes showing improved plant biomass production at limitingnitrogen growth conditions Plot Coverage Rosette Area Rosette Diameter[cm²] [cm²] [cm] Gene P- % P- % P- % Name Event # Ave. Val. Incr. Ave.Val. Incr. Ave. Val. Incr. LNU507 64087.1 33.8 0.29 8 4.2 0.29 8 3.40.12 5 LNU507 64584.2 — — — — — — 3.3 0.29 3 LNU479 65497.2 40.6 0.08 295.1 0.08 29 3.7 0.06 15 LNU479 65497.5 34.2 0.24 9 4.3 0.24 9 3.3 0.15 5LNU479 65499.1 37.1 0.10 18 4.6 0.10 18 3.5 0.23 10 LNU423 64102.1 — — —— — — 3.4 0.07 7 LNU418 65027.2 — — — — — — 3.5 0.13 11 LNU401 65493.234.1 0.28 8 4.3 0.28 8 3.4 0.09 6 LNU377 64603.2 — — — — — — 3.3 0.18 5LNU377 64604.3 — — — — — — 3.5 0.06 10 LNU368 64004.3 — — — — — — 3.30.25 4 LNU344 63520.4 — — — — — — 3.7 L 17 LNU344 63521.2 — — — — — —3.5 0.24 10 LNU339 65056.1 — — — — — — 3.5 0.17 9 LNU337 64952.1 35.80.10 14 4.5 0.10 14 3.5 0.03 10 LNU337 64954.1 — — — — — — 3.3 0.24 4LNU333 65295.1 36.9 0.22 17 4.6 0.22 17 3.6 L 13 LNU333 65297.1 35.30.24 12 4.4 0.24 12 3.5 0.04 11 LNU333 65297.2 — — — — — — 3.3 0.22 4LNU304 64573.1 — — — — — — 3.4 0.10 7 LNU304 64575.2 — — — — — — 3.30.24 4 LNU292 64084.1 36.1 0.06 15 4.5 0.06 15 3.6 L 13 CONT. — 31.5 — —3.9 — — 3.2 — — LNU507 64584.1 60.0 0.22 25 7.5 0.22 25 4.6 0.18 16LNU494 65302.1 58.3 0.12 21 7.3 0.12 21 4.4 0.14 11 LNU479 65499.1 58.00.15 21 7.2 0.15 21 4.3 0.17 10 LNU418 65024.2 75.2 0.08 57 9.4 0.08 575.1 L 31 LNU388 65487.1 62.5 0.04 30 7.8 0.04 30 4.6 0.05 16 LNU34864469.1 — — — — — — 4.3 0.23 9 LNU336 64447.2 — — — — — — 4.4 0.27 11LNU333 65295.2 — — — — — — 4.3 0.27 8 CONT. — 48.0 — — 6.0 — — 3.9 — —LNU508 64459.2 45.4 0.09 13 5.7 0.09 13 4.0 0.17 6 LNU469 64308.4 49.50.23 23 6.2 0.23 23 4.2 0.14 11 LNU469 64311.8 47.3 0.27 18 5.9 0.27 18— — — LNU460 64359.3 47.7 0.05 19 6.0 0.05 19 4.1 0.10 8 LNU460 64361.451.1 0.04 27 6.4 0.04 27 4.4 0.05 14 LNU459 64044.1 — — — — — — 4.1 0.236 LNU459 64541.4 — — — — — — 4.0 0.26 5 LNU459 64542.1 — — — — — — 4.10.24 7 LNU459 64542.4 49.4 0.19 23 6.2 0.19 23 4.2 0.14 11 LNU44264057.1 47.3 0.04 18 5.9 0.04 18 4.2 0.04 10 LNU442 64553.1 54.2 0.16 356.8 0.16 35 4.6 0.08 19 LNU421 64303.4 43.3 0.27 8 5.4 0.27 8 — — —LNU421 64304.4 50.3 0.02 25 6.3 0.02 25 4.3 0.02 13 LNU421 64305.11 — —— — — — 4.1 0.27 7 LNU420 64006.3 — — — — — — 4.0 0.22 6 LNU409 64688.2— — — 5.5 0.18 10 4.3 0.02 12 LNU408 64248.10 44.1 0.18 10 5.5 0.18 10 —— — LNU408 64249.4 46.1 0.06 15 5.8 0.06 15 4.1 0.08 8 LNU368 64004.2 —— — — — — 4.0 0.24 6 LNU363 64409.2 44.1 0.19 10 5.5 0.19 10 4.2 0.04 10LNU363 64410.1 51.6 0.06 29 6.5 0.06 29 4.2 0.16 10 LNU363 64413.2 44.10.19 10 5.5 0.19 10 — — — LNU331 64215.3 54.2 L 35 6.8 L 35 4.4 L 16LNU314 64434.2 54.7 0.03 36 6.8 0.03 36 4.4 0.03 16 LNU290 64368.4 46.80.04 17 5.9 0.04 17 4.1 0.09 8 LNU290 64369.3 43.7 0.22 9 5.5 0.22 9 — —— CONT. — 40.1 — — 5.0 — — 3.8 — — LNU517 64296.4 48.1 0.08 21 6.0 0.0821 4.2 0.21 8 LNU514 64365.2 44.1 0.26 11 5.5 0.26 11 — — — LNU50964692.3 50.5 L 27 6.3 L 27 4.5 L 14 LNU509 64692.6 48.4 0.02 22 6.0 0.0222 4.3 0.03 10 LNU504 64453.2 48.4 0.23 22 6.0 0.23 22 — — — LNU50164197.1 45.4 0.07 14 5.7 0.07 14 4.2 0.09 8 LNU397 64376.4 47.0 0.19 185.9 0.19 18 — — — LNU365 64708.2 44.9 0.18 13 5.6 0.18 13 4.2 0.22 8LNU365 64711.3 43.2 0.21 9 5.4 0.21 9 4.1 0.27 4 CONT. — 39.8 — — 5.0 —— 3.9 — — LNU513 63456.2 32.8 0.05 12 4.1 0.05 12 3.4 0.16 3 LNU51363458.2 35.8 0.18 21 4.5 0.18 21 3.7 0.07 13 LNU513 63459.2 38.5 L 314.8 L 31 3.8 L 16 LNU513 63460.2 33.6 0.05 14 4.2 0.05 14 3.6 L 8 LNU51263468.3 37.1 L 26 4.6 L 26 3.7 0.02 12 LNU512 63470.1 33.3 0.10 13 4.20.10 13 3.5 0.02 6 LNU512 63471.3 32.7 0.03 11 4.1 0.03 11 3.6 0.08 7LNU512 63471.4 37.0 0.01 26 4.6 0.01 26 3.8 0.03 15 LNU451 63499.1 32.30.05 10 4.0 0.05 10 3.5 0.04 5 LNU451 63499.5 38.4 L 30 4.8 L 30 3.8 L15 LNU451 63500.1 36.0 0.04 22 4.5 0.04 22 3.7 0.22 12 LNU424 63474.336.0 0.05 22 4.5 0.05 22 3.7 0.20 13 LNU424 63476.3 36.4 L 24 4.6 L 243.7 0.08 11 LNU424 63478.3 31.0 0.25 5 3.9 0.25 5 — — — LNU415 63692.132.2 0.17 9 4.0 0.17 9 — — — LNU411 63514.3 38.7 0.11 32 4.8 0.11 32 3.70.14 12 LNU411 63518.1 34.3 L 16 4.3 L 16 3.6 L 9 LNU375 63451.3 33.80.21 15 4.2 0.21 15 — — — LNU375 63452.2 33.8 0.04 15 4.2 0.04 15 3.60.07 9 LNU375 63454.1 33.9 0.01 15 4.2 0.01 15 3.6 0.14 8 LNU375 63454.236.7 0.16 25 4.6 0.16 25 3.8 0.07 14 LNU370 63544.3 36.5 0.06 24 4.60.06 24 3.8 L 14 LNU370 63545.6 35.7 0.17 21 4.5 0.17 21 3.7 0.05 11LNU370 63548.2 36.0 L 22 4.5 L 22 3.8 L 14 LNU357 63533.1 41.0 0.07 395.1 0.07 39 3.9 L 19 LNU357 63533.8 35.5 L 21 4.4 L 21 3.6 L 9 LNU35763534.1 39.5 0.03 34 4.9 0.03 34 3.9 L 18 LNU356 63444.2 35.3 0.03 204.4 0.03 20 3.7 L 10 LNU356 63445.1 35.2 0.07 20 4.4 0.07 20 3.7 0.03 12LNU351 63462.3 35.2 0.16 20 4.4 0.16 20 3.6 L 10 LNU351 63463.2 38.4 L30 4.8 L 30 3.8 L 15 LNU351 63464.1 — — — — — — 3.5 0.27 6 LNU34463520.2 33.4 0.02 13 4.2 0.02 13 3.5 0.02 6 LNU344 63521.1 34.8 0.12 184.3 0.12 18 3.6 0.23 10 LNU344 63521.2 33.5 0.08 14 4.2 0.08 14 3.5 0.195 LNU330 63438.1 35.6 0.11 21 4.5 0.11 21 3.7 0.04 11 LNU330 63439.132.5 0.08 10 4.1 0.08 10 3.5 L 7 LNU326 63433.2 32.4 0.08 10 4.1 0.08 103.5 0.03 5 LNU326 63433.4 35.3 L 20 4.4 L 20 3.6 L 9 LNU326 63434.1 — —— — — — 3.6 0.27 8 LNU326 63435.1 40.1 0.03 36 5.0 0.03 36 4.0 L 20LNU319 63527.1 35.4 L 20 4.4 L 20 3.7 L 12 LNU319 63527.2 35.7 0.28 214.5 0.28 21 3.7 0.21 10 LNU319 63528.1 37.8 0.15 29 4.7 0.15 29 3.8 0.2015 LNU319 63530.3 34.9 0.08 19 4.4 0.08 19 3.6 0.24 9 LNU308 63414.133.9 0.03 15 4.2 0.03 15 3.6 0.04 10 LNU308 63414.4 37.3 0.02 27 4.70.02 27 3.9 L 18 LNU308 63417.5 37.1 L 26 4.6 L 26 3.8 L 15 LNU30863417.8 37.3 L 27 4.7 L 27 3.7 L 12 LNU302 63378.3 34.4 0.22 17 4.3 0.2217 3.6 0.06 7 LNU302 63381.1 40.5 L 38 5.1 L 38 3.9 L 18 LNU302 63382.240.4 L 37 5.0 L 37 3.9 L 17 LNU291 63385.2 37.6 0.19 28 4.7 0.19 28 3.70.25 12 LNU291 63387.1 34.1 0.11 16 4.3 0.11 16 3.7 0.05 11 LNU29163387.3 36.3 0.22 23 4.5 0.22 23 3.8 0.18 14 LNU291 63388.1 37.6 L 284.7 L 28 3.8 L 14 CONT. — 29.4 — — 3.7 — — 3.3 — — LNU469 64308.5 — — —— — — 4.2 0.27 3 LNU469 64313.9 51.7 0.12 13 6.5 0.12 13 4.3 0.06 6LNU444 64182.3 52.0 0.04 13 6.5 0.04 13 4.3 0.08 7 LNU442 64555.1 56.5 L23 7.1 L 23 4.4 0.02 8 LNU430 63935.1 51.9 0.08 13 6.5 0.08 13 4.4 0.1210 LNU391 63980.3 50.5 0.11 10 6.3 0.11 10 4.2 0.17 5 LNU366 64027.752.6 0.03 15 6.6 0.03 15 4.3 0.03 8 LNU366 64028.3 52.4 0.14 14 6.5 0.1414 — — — LNU363 64409.3 — — — — — — 4.2 0.23 5 LNU314 64433.3 50.3 0.2510 6.3 0.25 10 4.3 0.06 6 LNU314 64437.2 — — — — — — 4.2 0.18 4 CONT. —45.8 — — 5.7 — — 4.0 — — LNU492 64177.2 40.3 0.20 7 5.0 0.20 7 — — —LNU471 64838.1 42.1 0.12 12 5.3 0.12 12 3.6 0.09 5 LNU463 64283.4 49.90.07 32 6.2 0.07 32 4.0 0.10 17 LNU454 64796.3 44.5 0.01 18 5.6 0.01 183.7 0.02 8 LNU454 64799.2 39.8 0.30 6 5.0 0.30 6 — — — LNU413 65019.141.8 0.07 11 5.2 0.07 11 3.7 0.03 7 LNU413 65022.4 48.8 0.18 30 6.1 0.1830 3.8 0.26 11 LNU410 64974.3 44.9 L 19 5.6 L 19 3.7 0.01 9 LNU38764808.1 41.1 0.12 9 5.1 0.12 9 3.5 0.20 3 LNU387 64810.4 45.4 0.12 205.7 0.12 20 3.7 0.23 7 LNU382 64429.3 40.1 0.24 6 5.0 0.24 6 3.5 0.28 3LNU373 64826.4 44.6 0.07 18 5.6 0.07 18 3.7 0.04 9 LNU373 64830.1 46.00.12 22 5.7 0.12 22 3.7 0.07 8 LNU361 64832.1 43.7 0.19 16 5.5 0.19 163.6 0.08 6 LNU355 65012.1 45.9 0.12 22 5.7 0.12 22 3.8 L 12 LNU35565014.2 41.4 0.11 10 5.2 0.11 10 3.7 0.03 7 LNU355 65015.2 46.0 L 22 5.8L 22 3.8 L 10 LNU332 64824.4 40.3 0.22 7 5.0 0.22 7 — — — LNU307 64958.241.8 0.07 11 5.2 0.07 11 — — — LNU307 64960.2 — — — — — — 3.7 0.24 8LNU303 65046.3 40.3 0.29 7 5.0 0.29 7 3.6 0.13 4 CONT. — 37.7 — — 4.7 —— 3.4 — — LNU517 64296.3 51.9 0.02 26 6.5 0.02 26 4.3 0.04 11 LNU51764297.9 49.5 0.03 20 6.2 0.03 20 4.1 0.06 8 LNU514 64364.2 55.1 L 33 6.9L 33 4.5 L 18 LNU514 64365.3 52.3 0.09 27 6.5 0.09 27 4.4 L 14 LNU51464366.1 48.2 0.11 17 6.0 0.11 17 4.2 0.03 10 LNU509 64692.3 47.3 0.06 155.9 0.06 15 4.2 0.02 10 LNU501 64197.1 45.4 0.23 10 5.7 0.23 10 — — —LNU501 64197.3 45.5 0.15 10 5.7 0.15 10 4.0 0.22 5 LNU501 64723.1 52.00.11 26 6.5 0.11 26 4.3 0.19 12 LNU461 64668.5 44.4 0.26 8 5.6 0.26 84.2 0.05 9 LNU397 64375.1 52.2 0.02 26 6.5 0.02 26 4.3 0.01 12 LNU39664315.13 45.9 0.12 11 5.7 0.12 11 — — — LNU396 64317.3 49.6 0.02 20 6.20.02 20 4.2 0.13 9 LNU386 64394.3 — — — — — — 4.0 0.30 5 LNU372 64481.149.1 0.02 19 6.1 0.02 19 4.3 0.02 11 LNU372 64483.3 53.5 L 29 6.7 L 294.3 0.08 12 LNU369 64386.1 49.9 0.16 21 6.2 0.16 21 4.2 0.09 10 LNU36964387.1 52.1 0.12 26 6.5 0.12 26 4.3 0.08 13 LNU369 64387.2 48.4 0.24 176.1 0.24 17 — — — LNU369 64389.2 52.7 L 28 6.6 L 28 4.4 L 14 LNU35064676.2 — — — — — — 4.1 0.21 7 LNU350 64677.2 — — — — — — 4.0 0.29 4LNU345 64335.1 — — — — — — 4.1 0.28 7 LNU342 64035.1 45.9 0.14 11 5.70.14 11 4.0 0.27 5 LNU342 64035.3 — — — — — — 4.3 0.12 11 LNU342 64035.8— — — — — — 4.0 0.23 5 LNU313 64661.8 49.6 0.09 20 6.2 0.09 20 4.2 0.279 LNU313 64664.1 50.0 0.02 21 6.3 0.02 21 4.2 0.03 10 LNU313 64664.348.6 0.07 18 6.1 0.07 18 4.1 0.06 8 CONT. — 41.3 — — 5.2 — — 3.8 — —LNU477 63888.1 — — — — — — 3.8 0.23 3 LNU472 63921.4 — — — — — — 3.80.22 4 LNU439 64616.2 38.5 0.24 9 4.8 0.24 9 — — — LNU419 63896.2 37.10.14 5 4.6 0.14 5 — — — LNU407 64219.1 37.2 0.08 6 4.6 0.08 6 3.9 L 8LNU403 64237.1 41.4 L 17 5.2 L 17 4.0 L 9 LNU393 63978.2 37.7 0.03 7 4.70.03 7 3.8 0.06 4 LNU374 63997.2 38.3 0.01 9 4.8 0.01 9 3.8 0.15 5LNU335 64169.2 41.2 0.12 17 5.2 0.12 17 3.9 0.09 9 CONT. — 35.2 — — 4.4— — 3.6 — — LNU500 64222.1 45.4 0.09 7 5.7 0.09 7 — — — LNU496 64195.646.1 0.05 9 5.8 0.05 9 4.3 0.08 8 LNU472 63949.7 45.3 0.22 7 5.7 0.22 74.2 L 7 LNU419 63897.6 46.3 0.21 9 5.8 0.21 9 — — — LNU343 64208.1 49.5L 17 6.2 L 17 4.3 L 8 LNU343 64209.1 — — — — — — 4.1 0.29 3 LNU32864151.2 43.6 0.15 3 5.4 0.15 3 4.1 0.26 3 LNU327 64491.2 48.1 0.03 136.0 0.03 13 4.2 0.18 6 LNU305 64111.3 45.1 0.17 6 5.6 0.17 6 — — — CONT.— 42.4 — — 5.3 — — 4.0 — — LNU503 64203.3 57.1 0.26 12 7.1 0.26 12 4.60.16 7 LNU430 63935.1 — — — — — — 4.5 0.27 5 LNU430 63952.1 60.0 0.13 187.5 0.13 18 4.6 0.09 8 LNU403 64237.1 63.6 0.04 25 7.9 0.04 25 4.8 0.1212 LNU366 64028.3 — — — — — — 4.6 0.19 7 LNU353 64032.3 58.3 0.22 15 7.30.22 15 — — — LNU352 64199.1 58.1 0.19 14 7.3 0.19 14 4.7 0.06 10 LNU35264200.10 62.4 0.06 23 7.8 0.06 23 4.9 0.02 14 LNU335 64168.19 67.9 0.0534 8.5 0.05 34 5.0 0.02 17 CONT. — 50.8 — — 6.4 — — 4.3 — — LNU49964146.11 70.5 0.20 6 8.8 0.20 6 — — — LNU499 64146.7 — — — — — — 5.00.26 3 LNU499 64146.8 68.8 0.08 3 8.6 0.08 3 5.0 0.05 3 LNU468 63492.283.4 0.05 25 10.4 0.05 25 5.6 0.07 14 LNU468 63492.3 77.1 0.30 16 9.60.30 16 5.4 0.29 11 LNU467 63714.4 70.9 0.11 6 8.9 0.11 6 5.1 0.08 4LNU467 63718.2 — — — — — — 5.0 0.10 3 LNU455 64187.5 — — — — — — 5.00.10 2 LNU455 64189.2 70.8 0.05 6 8.8 0.05 6 5.2 L 6 LNU455 64189.7 88.90.14 33 11.1 0.14 33 5.7 0.21 17 LNU450 63708.3 69.7 0.03 4 8.7 0.03 45.1 0.02 4 LNU450 63708.6 75.3 0.10 13 9.4 0.10 13 5.3 0.06 8 LNU44863705.2 — — — — — — 5.2 0.21 7 LNU448 63706.5 70.6 0.13 6 8.8 0.13 6 5.00.30 3 LNU429 63937.4 72.7 0.21 9 9.1 0.21 9 — — — LNU429 63938.8 71.0 L6 8.9 L 6 5.1 0.02 4 LNU425 63911.11 — — — — — — 5.1 0.01 4 LNU42563911.7 72.9 L 9 9.1 L 9 5.1 L 5 LNU402 63914.2 71.9 0.09 8 9.0 0.09 8 —— — LNU399 63944.6 72.4 L 8 9.0 L 8 5.1 0.06 5 LNU395 64142.8 — — — — —— 5.1 0.16 5 LNU392 63696.2 73.3 L 10 9.2 L 10 — — — LNU392 63697.4 — —— — — — 5.2 0.28 6 LNU392 63698.2 — — — — — — 5.2 0.26 6 LNU392 63700.370.9 0.23 6 8.9 0.23 6 5.2 L 6 LNU390 63538.1 72.3 0.05 8 9.0 0.05 8 5.10.01 5 LNU390 63539.3 — — — — — — 5.2 0.25 7 LNU390 63539.4 78.0 0.09 179.8 0.09 17 5.4 0.11 10 LNU349 63989.1 — — — — — — 5.0 0.26 2 LNU34963989.5 75.1 L 12 9.4 L 12 5.2 0.23 7 LNU347 63510.4 73.9 0.14 11 9.20.14 11 5.2 L 6 LNU347 63513.3 72.6 0.04 9 9.1 0.04 9 5.1 0.04 4 LNU34763513.4 — — — — — — 5.2 0.20 6 LNU329 63427.3 — — — — — — 5.4 0.22 11LNU329 63429.1 84.8 0.21 27 10.6 0.21 27 5.6 0.14 14 LNU323 63421.4 72.80.20 9 9.1 0.20 9 5.1 0.02 4 LNU323 63424.4 69.6 0.22 4 8.7 0.22 4 5.10.25 5 CONT. — 66.7 — — 8.3 — — 4.9 — — LNU476 64041.2 — — — — — — 3.30.24 3 LNU410 64971.1 35.9 L 25 4.5 L 25 3.6 L 14 LNU387 64810.4 31.30.19 9 3.9 0.19 9 3.3 0.19 3 LNU382 64429.3 37.9 0.18 32 4.7 0.18 32 3.60.19 14 LNU373 64828.1 32.0 0.17 11 4.0 0.17 11 — — — LNU373 64830.131.3 0.25 9 3.9 0.25 9 3.3 0.25 3 LNU355 65013.2 36.6 L 28 4.6 L 28 3.6L 13 LNU355 65014.2 — — — — — — 3.3 0.10 4 LNU307 64959.2 34.2 0.08 194.3 0.08 19 3.4 0.02 9 LNU303 65043.1 35.7 0.01 24 4.5 0.01 24 3.4 0.247 LNU300 65032.1 32.5 0.21 13 4.1 0.21 13 — — — CONT. — 28.7 — — 3.6 — —3.2 — — LNU513 63458.3 58.4 0.28 10 7.3 0.28 10 — — — LNU512 63468.367.2 0.02 27 8.4 0.02 27 4.9 0.01 12 LNU512 63470.1 59.2 0.12 12 7.40.12 12 4.6 0.21 5 LNU424 63476.3 68.2 0.03 29 8.5 0.03 29 5.0 L 13LNU424 63478.3 57.4 0.29 9 7.2 0.29 9 — — — LNU415 63691.2 59.5 0.11 137.4 0.11 13 4.6 0.13 6 LNU411 63514.3 60.9 0.07 15 7.6 0.07 15 4.7 0.087 LNU411 63517.1 57.1 0.27 8 7.1 0.27 8 4.7 0.09 7 LNU375 63452.2 61.20.14 16 7.7 0.14 16 4.7 0.18 7 LNU375 63452.3 — — — — — — 4.6 0.17 6LNU375 63454.1 62.5 0.04 18 7.8 0.04 18 4.7 0.15 6 LNU375 63454.2 — — —— — — 4.7 0.29 6 LNU370 63544.3 — — — — — — 4.6 0.13 6 LNU370 63545.259.9 0.11 13 7.5 0.11 13 4.7 0.09 7 LNU357 63532.3 62.8 0.07 19 7.9 0.0719 4.8 0.12 8 LNU357 63533.1 75.8 L 43 9.5 L 43 5.1 L 17 LNU357 63533.862.4 0.04 18 7.8 0.04 18 4.7 0.07 8 LNU356 63444.1 61.0 0.08 15 7.6 0.0815 4.8 0.05 9 LNU356 63444.3 61.8 0.12 17 7.7 0.12 17 4.7 0.23 8 LNU35663445.1 67.8 L 28 8.5 L 28 5.0 L 15 LNU351 63462.3 59.8 0.10 13 7.5 0.1013 4.7 0.11 6 LNU351 63464.1 62.4 0.04 18 7.8 0.04 18 4.8 0.05 8 LNU34463520.4 64.4 0.02 22 8.0 0.02 22 4.8 0.02 11 LNU330 63438.1 60.3 0.13 147.5 0.13 14 4.6 0.20 6 LNU330 63439.1 — — — — — — 4.6 0.21 5 LNU33063441.2 62.1 0.07 17 7.8 0.07 17 4.8 0.23 10 LNU319 63527.1 74.1 L 409.3 L 40 5.2 L 18 LNU319 63528.1 67.9 L 28 8.5 L 28 4.9 0.01 12 LNU31963530.1 59.6 0.27 13 7.5 0.27 13 — — — LNU319 63530.3 58.1 0.28 10 7.30.28 10 — — — LNU302 63379.1 58.9 0.14 11 7.4 0.14 11 4.7 0.06 8 CONT. —52.9 — — 6.6 — — 4.4 — — Table 92: “CONT.”—Control; “Ave.”—Average; “%Incr.” = % increment; “p-val.”—p-value; L means that p-value is lessthan 0.01, p < 0.1 was considered as significant.

The genes listed in Table 93 improved plant NUE when grown at limitingnitrogen concentration levels. These genes produced faster developingplants when grown under limiting nitrogen growth conditions, compared tocontrol plants as measured by growth rate of leaf number, rosettediameter and plot coverage.

TABLE 93 Genes showing improved rosette growth performance at limitingnitrogen growth conditions RGR Of Leaf RGR Of Plot RGR Of Rosette NumberCoverage Diameter Gene P- % P- % P- % Name Event # Ave. Val. Incr. Ave.Val. Incr. Ave. Val. Incr. LNU479 65497.2 — — — 5.1 L 33 0.3 0.05 26LNU479 65499.1 — — — 4.5 0.09 20 — — — LNU418 65025.1 — — — — — — 0.30.15 20 LNU418 65027.2 — — — — — — 0.3 0.22 17 LNU401 65493.2 — — — — —— 0.3 0.26 15 LNU401 65494.1 — — — 4.4 0.17 17 — — — LNU377 64603.2 — —— — — — 0.3 0.19 17 LNU377 64604.3 — — — — — — 0.3 0.21 17 LNU34463520.4 — — — — — — 0.3 0.07 27 LNU344 63521.2 — — — 4.5 0.15 18 0.30.21 18 LNU339 65056.1 — — — — — — 0.3 0.26 15 LNU337 64952.1 — — — 4.40.16 16 0.3 0.13 21 LNU333 65295.1 — — — 4.5 0.12 18 0.3 0.19 18 LNU33365297.1 — — — 4.4 0.17 16 0.3 0.03 29 LNU324 64233.4 — — — 4.7 0.09 250.3 0.20 21 LNU324 64234.5 — — — 4.3 0.26 15 0.3 0.12 24 LNU318 65066.6— — — — — — 0.3 0.19 18 LNU304 64573.1 — — — — — — 0.3 0.23 15 LNU29264084.1 — — — 4.5 0.11 18 0.3 0.09 23 CONT. — — — — 3.8 — — 0.2 — —LNU507 64584.1 — — — 6.4 0.16 39 0.3 0.15 44 LNU494 65302.1 — — — 6.20.20 33 0.3 0.21 37 LNU423 64596.1 0.8 0.16 43 — — — — — — LNU41865024.2 — — — 8.4 L 81 0.4 0.02 72 LNU388 65487.1 — — — 6.3 0.19 35 — —— LNU377 64604.3 0.7 0.19 42 — — — — — — LNU348 64469.1 0.7 0.24 36 — —— 0.3 0.26 34 LNU339 65056.1 0.8 0.08 54 — — — — — — LNU333 65297.1 0.80.16 45 6.7 0.20 44 — — — CONT. — 0.5 — — 4.6 — — 0.2 — — LNU508 64459.2— — — 5.6 0.26 12 — — — LNU469 64308.4 — — — 5.9 0.11 20 — — — LNU46964311.8 — — — 5.7 0.19 16 — — — LNU460 64359.3 — — — 5.8 0.12 18 — — —LNU460 64360.1 — — — — — — 0.3 0.13 18 LNU460 64361.4 — — — 6.4 0.02 280.4 0.09 20 LNU459 64044.1 — — — — — — 0.3 0.23 14 LNU459 64542.1 — — —— — — 0.3 0.12 18 LNU459 64542.4 — — — 6.1 0.06 23 0.3 0.23 14 LNU44264057.1 0.7 0.29 19 6.0 0.08 21 0.4 0.09 20 LNU442 64553.1 — — — 6.8 L38 0.4 0.05 25 LNU439 64618.3 — — — — — — 0.3 0.26 15 LNU421 64304.4 — —— 6.3 0.03 26 — — — LNU421 64305.11 — — — 5.7 0.22 15 0.3 0.25 13 LNU41664134.11 — — — — — — 0.3 0.26 13 LNU409 64684.2 — — — — — — 0.3 0.15 17LNU409 64688.2 — — — — — — 0.4 0.01 31 LNU408 64249.4 — — — 5.8 0.14 17— — — LNU368 64004.2 — — — 5.6 0.29 13 — — — LNU363 64409.2 — — — — — —0.4 0.05 23 LNU363 64410.1 — — — 6.3 0.03 28 — — — LNU331 64215.3 — — —6.7 L 35 0.3 0.12 18 LNU314 64434.2 — — — 6.7 L 36 0.3 0.15 17 LNU31464437.6 — — — — — — 0.3 0.26 13 LNU290 64368.4 — — — 5.9 0.13 18 0.30.16 17 CONT. — 0.6 — — 5.0 — — 0.3 — — LNU517 64296.4 — — — 5.9 0.17 21— — — LNU509 64692.3 — — — 6.4 0.05 30 0.4 0.07 21 LNU509 64692.6 — — —5.9 0.15 21 — — — LNU509 64695.1 — — — — — — 0.4 0.28 13 LNU504 64453.2— — — 5.9 0.19 20 — — — LNU501 64197.1 — — — 5.7 0.26 17 0.4 0.16 16LNU397 64376.4 — — — 5.9 0.18 20 — — — LNU386 64392.4 0.6 0.29 17 — — —— — — LNU342 64036.2 0.6 0.29 17 — — — — — — CONT. — 0.5 — — 4.9 — — 0.3— — LNU513 63458.2 — — — 4.5 0.17 19 0.3 0.14 11 LNU513 63459.2 — — —4.8 0.05 28 0.3 0.14 10 LNU513 63460.2 — — — — — — 0.3 0.22  8 LNU51263468.3 — — — 4.7 0.10 24 — — — LNU512 63471.4 — — — 4.7 0.08 25 0.30.04 15 LNU451 63499.1 — — — — — — 0.3 0.22  9 LNU451 63499.5 — — — 4.90.05 29 0.3 0.06 14 LNU451 63500.1 — — — 4.6 0.12 22 0.3 0.06 14 LNU42463474.3 — — — 4.5 0.14 20 — — — LNU424 63476.3 — — — 4.6 0.12 22 0.30.18 10 LNU424 63478.1 0.7 0.17 18 — — — — — — LNU415 63691.2 0.7 0.2416 — — — — — — LNU411 63514.3 — — — 5.0 0.04 32 0.3 0.05 14 LNU41163518.1 — — — 4.4 0.25 16 0.3 0.13 11 LNU375 63451.3 — — — 4.3 0.30 150.3 0.10 12 LNU375 63452.2 — — — 4.4 0.24 17 0.3 0.05 15 LNU375 63452.30.7 0.22 18 — — — — — — LNU375 63454.1 0.7 0.11 19 4.3 0.26 15 0.3 0.24 8 LNU375 63454.2 — — — 4.7 0.10 24 0.3 0.02 17 LNU370 63544.3 — — — 4.70.10 24 0.3 0.07 13 LNU370 63545.2 — — — — — — 0.3 0.16 10 LNU37063545.6 — — — 4.6 0.12 23 0.3 0.06 14 LNU370 63547.1 0.7 0.24 15 — — — —— — LNU370 63548.2 — — — 4.6 0.13 21 0.3 0.10 11 LNU357 63533.1 — — —5.2 0.01 38 0.3 0.05 15 LNU357 63533.8 0.7 0.10 21 4.5 0.15 20 — — —LNU357 63534.1 0.7 0.18 16 5.0 0.03 34 0.3 0.01 18 LNU356 63444.1 — — —— — — 0.3 0.26  8 LNU356 63444.2 — — — 4.5 0.17 19 0.3 0.18  9 LNU35663445.1 — — — 4.5 0.19 19 0.3 0.09 12 LNU351 63462.1 — — — 4.4 0.29 160.3 0.11 12 LNU351 63462.3 — — — 4.5 0.16 20 0.3 0.11 11 LNU351 63463.2— — — 5.0 0.04 32 0.3 L 19 LNU351 63464.1 0.7 0.24 17 — — — 0.3 0.20  9LNU351 63466.1 — — — — — — 0.3 0.14 13 LNU344 63521.1 — — — 4.5 0.20 180.3 0.12 11 LNU330 63438.1 — — — 4.6 0.13 22 0.3 0.08 13 LNU330 63439.1— — — — — — 0.3 0.24  8 LNU330 63440.2 — — — — — — 0.3 0.17 11 LNU33063441.1 0.7 0.20 19 — — — — — — LNU326 63433.2 — — — — — — 0.3 0.23  8LNU326 63433.4 — — — 4.5 0.15 21 0.3 0.11 11 LNU326 63434.1 0.7 0.27 14— — — 0.3 0.18 10 LNU326 63435.1 — — — 5.1 0.02 36 0.3 0.01 18 LNU31963527.1 — — — 4.4 0.20 18 0.3 0.27  8 LNU319 63527.2 0.7 0.23 15 4.60.14 22 0.3 0.15 10 LNU319 63528.1 — — — 4.8 0.06 28 0.3 0.07 15 LNU31963530.3 — — — 4.4 0.22 18 0.3 0.19 10 LNU308 63414.1 — — — 4.4 0.25 160.3 0.05 14 LNU308 63414.4 — — — 4.7 0.08 26 0.3 0.02 17 LNU308 63417.50.7 0.25 14 4.8 0.06 27 0.3 L 20 LNU308 63417.8 — — — 4.8 0.07 27 0.30.08 12 LNU302 63378.3 — — — 4.4 0.25 16 0.3 0.19  9 LNU302 63379.1 — —— 4.4 0.25 17 0.3 0.27  8 LNU302 63380.1 — — — 4.7 0.11 26 0.4 0.01 24LNU302 63381.1 — — — 5.2 0.01 38 0.3 L 20 LNU302 63382.2 — — — 5.2 0.0138 0.3 0.02 17 LNU291 63385.1 — — — 4.3 0.29 15 0.3 0.14 12 LNU29163385.2 0.7 0.18 18 4.8 0.06 29 0.3 0.12 11 LNU291 63387.1 0.7 0.17 184.3 0.28 15 0.3 0.18  9 LNU291 63387.3 0.7 0.22 15 4.6 0.11 23 0.3 0.0316 LNU291 63388.1 — — — 4.8 0.05 28 0.3 0.07 12 CONT. — 0.6 — — 3.8 — —0.3 — — LNU444 64182.1 — — — 6.6 0.26 16 — — — LNU444 64182.3 0.7 0.2819 — — — — — — LNU442 64553.1 0.7 0.28 18 — — — — — — LNU442 64555.1 — —— 7.0 0.09 23 — — — LNU430 63935.1 — — — — — — 0.4 0.25 12 CONT. — 0.6 —— 5.7 — — 0.3 — — LNU463 64283.4 0.7 0.26 39 4.4 0.24 44 0.2 0.21 38LNU413 65022.4 — — — 4.3 0.26 42 — — — LNU373 64828.1 — — — 4.1 0.29 37— — — LNU373 64830.1 — — — 4.2 0.28 39 — — — LNU355 65012.1 — — — 4.20.29 38 0.2 0.30 30 CONT. — 0.5 — — 3.0 — — 0.2 — — LNU517 64296.3 0.70.11 24 6.5 0.06 23 — — — LNU517 64297.9 — — — 6.3 0.12 18 — — — LNU51464364.2 — — — 7.0 0.01 31 0.4 0.28 11 LNU514 64365.3 0.6 0.25 20 6.70.04 25 0.4 0.26 11 LNU514 64366.1 0.7 0.09 28 6.1 0.21 15 — — — LNU50964690.3 0.7 0.10 28 6.2 0.17 17 — — — LNU509 64690.6 0.7 0.19 24 — — — —— — LNU509 64692.3 — — — — — — 0.4 0.30 10 LNU501 64723.1 — — — 6.6 0.0524 — — — LNU397 64375.1 — — — 6.8 0.03 27 0.4 0.19 13 LNU396 64317.3 — —— 6.3 0.13 18 — — — LNU386 64394.3 0.6 0.29 18 — — — — — — LNU38164285.5 0.7 0.17 24 — — — — — — LNU372 64481.1 — — — 6.3 0.12 18 — — —LNU372 64483.3 — — — 6.8 0.03 27 — — — LNU369 64386.1 — — — 6.3 0.13 19— — — LNU369 64387.1 0.7 0.18 26 6.6 0.05 24 — — — LNU369 64387.2 0.60.25 18 6.1 0.21 15 — — — LNU369 64389.2 — — — 6.7 0.04 25 — — — LNU36564708.1 0.7 0.16 23 — — — — — — LNU365 64711.3 0.7 0.08 32 — — — — — —LNU350 64674.4 — — — 6.2 0.21 17 — — — LNU342 64035.3 — — — 6.0 0.26 14— — — LNU313 64661.8 — — — 6.4 0.11 20 — — — LNU313 64663.2 0.6 0.18 22— — — — — — LNU313 64664.1 — — — 6.4 0.10 20 — — — LNU313 64664.3 0.60.20 22 6.3 0.14 18 — — — LNU294 64658.7 — — — 6.2 0.25 16 — — — CONT. —0.5 — — 5.3 — — 0.3 — — LNU403 64237.1 — — — 5.2 0.05 17 — — — LNU33564169.2 — — — 5.2 0.05 18 — — — CONT. — — — — 4.4 — — — — — LNU51864016.4 — — — 6.0 0.27 13 — — — LNU496 64195.1 0.7 0.07 31 — — — 0.40.09  8 LNU496 64195.6 — — — 5.9 0.26 11 0.4 L 15 LNU493 64190.1 0.60.25 22 — — — — — — LNU493 64191.3 0.6 0.29 23 — — — — — — LNU48164140.1 — — — — — — 0.4 0.15  8 LNU472 63949.7 — — — 5.8 0.30 10 0.4 L15 LNU458 63895.1 0.7 0.19 28 6.0 0.19 13 0.4 0.02 13 LNU343 64208.1 — —— 6.2 0.05 18 0.4 0.13  7 LNU343 64209.2 0.6 0.29 21 — — — — — — LNU34064290.11 — — — — — — 0.4 0.09  8 LNU327 64490.2 — — — — — — 0.4 0.04 11LNU327 64491.2 — — — 6.0 0.15 14 — — — LNU306 64132.2 0.7 0.16 25 — — —— — — LNU305 64111.3 0.7 0.21 26 — — — — — — CONT. — 0.5 — — 5.3 — — 0.3— — LNU403 64237.1 — — — 8.3 0.19 24 — — — LNU353 64033.2 0.8 0.30 12 —— — — — — LNU352 64200.10 — — — 8.2 0.23 22 — — — LNU335 64168.19 — — —9.0 0.08 35 0.5 0.16 18 LNU317 64094.1 0.8 0.21 14 — — — — — — CONT. —0.7 — — 6.7 — — 0.4 — — LNU468 63492.2 0.9 0.03 18 10.9  0.08 26 0.50.04 16 LNU468 63492.3 — — — 10.0  0.25 16 0.5 0.10 13 LNU462 63502.20.9 0.04 19 — — — — — — LNU462 63505.1 0.8 0.28 9 — — — — — — LNU45564187.4 — — — 10.4  0.17 20 0.5 0.23 11 LNU455 64189.2 — — — — — — 0.50.28  8 LNU455 64189.7 — — — 11.7  0.02 35 0.5 0.04 17 LNU450 63708.6 —— — — — — 0.5 0.24  9 LNU450 63710.2 0.9 0.12 12 — — — — — — LNU42963938.8 0.8 0.22 9 — — — — — — LNU425 63911.12 — — — — — — 0.5 0.28 11LNU395 64145.4 0.8 0.25 10 — — — — — — LNU390 63539.2 0.9 0.17 13 — — —— — — LNU390 63539.4 — — — 10.1 0.22 17 0.5 0.27  8 LNU347 63508.1 0.90.12 12 — — — — — — LNU347 63510.4 0.9 0.08 14 — — — — — — LNU34763513.3 0.8 0.27 10 — — — — — — LNU347 63513.4 — — — — — — 0.5 0.27  8LNU329 63427.3 — — — 10.3  0.20 19 0.5 0.11 14 LNU329 63429.1 0.8 0.2510 11.1  0.07 28 0.5 0.05 16 LNU323 63424.1 — — — 10.2  0.21 18 0.5 0.29 9 CONT. — 0.8 — — 8.7 — — 0.5 — — LNU471 64838.1 0.7 0.18 25 — — — — —— LNU410 64971.1 — — — 4.3 0.03 29 0.3 0.04 30 LNU410 64972.4 — — — 4.10.16 21 0.3 0.19 20 LNU382 64429.3 — — — 4.5 0.02 34 0.2 0.25 17 LNU37364828.1 0.7 0.19 25 — — — — — — LNU361 64834.1 0.7 0.23 26 — — — — — —LNU355 65013.2 — — — 4.4 0.02 31 0.3 0.05 26 LNU307 64958.2 — — — 3.90.26 15 — — — LNU307 64959.2 — — — 4.2 0.05 25 0.3 0.12 19 LNU30365043.1 0.7 0.18 25 4.3 0.03 27 — — — LNU303 65046.3 0.7 0.14 26 — — — —— — LNU300 65032.1 — — — 3.9 0.19 16 0.2 0.18 17 CONT. — 0.5 — — 3.4 — —0.2 — — LNU512 63468.3 — — — 8.6 0.12 27 — — — LNU424 63476.3 — — — 8.80.10 30 0.5 0.20 13 LNU357 63532.3 — — — 8.1 0.26 19 — — — LNU35763533.1 0.9 0.28 17 9.7 0.02 42 — — — LNU357 63533.8 — — — 8.0 0.29 18 —— — LNU356 63445.1 — — — 8.7 0.11 28 — — — LNU351 63464.1 — — — 8.0 0.2818 — — — LNU344 63520.4 — — — 8.3 0.20 22 — — — LNU319 63527.1 — — — 9.50.03 40 0.5 0.19 13 LNU319 63528.1 — — — 8.8 0.09 29 — — — LNU29163385.1 — — — 8.4 0.20 23 — — — CONT. — 0.7 — — 6.8 — — 0.4 — — Table93. “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment; “p-val.”—p-value; L means that p-value is less than 0.01, p < 0.1 was consideredas significant.

The genes listed in Tables 94-95 improved plant NUE when grown atstandard nitrogen concentration levels. These genes produced largerplants with a larger photosynthetic area and increased biomass (freshweight, dry weight, leaf number, rosette diameter, rosette area and plotcoverage) when grown under standard nitrogen conditions.

TABLE 94 Genes showing improved plant biomass production at standardnitrogen growth conditions Dry Weight [mg] Fresh Weight [mg] Leaf NumberGene P- % P- % P- % Name Event # Ave. Val. Incr. Ave. Val. Incr. Ave.Val. Incr. LNU507 64584.2 60.0 0.10 36 706.2 0.06 37 — — — LNU50764585.2 53.1 0.16 21 712.5 0.05 38 — — — LNU494 65299.1 — — — 618.8 0.2720 — — — LNU479 65497.5 61.9 0.01 41 762.5 0.03 48 10.9 0.20 9 LNU47965499.1 — — — — — — 10.8 0.27 8 LNU418 65027.2 62.5 0.03 42 787.5 0.0253 11.0 0.05 10  LNU401 65493.2 51.9 0.17 18 — — — — — — LNU401 65493.3— — — 643.8 0.18 25 — — — LNU401 65494.1 — — — 768.8 0.22 49 — — —LNU388 65487.1 54.4 0.12 24 — — — — — — LNU377 64604.3 — — — — — — 10.50.26 5 LNU377 64604.6 60.6 0.12 38 706.2 0.06 37 — — — LNU368 64004.356.2 0.05 28 718.8 0.05 40 10.7 0.16 7 LNU368 64005.1 52.5 0.30 19 — — —— — — LNU344 63521.1 61.2 0.04 39 806.2 0.02 57 11.2 0.03 12  LNU33965055.1 55.6 0.15 27 656.2 0.14 28 — — — LNU337 64953.1 — — — — — — 11.50.19 15  LNU337 64954.1 — — — — — — 11.2 0.03 12  LNU337 64955.2 — — —656.2 0.14 28 — — — LNU333 65298.4 50.6 0.22 15 — — — — — — LNU32464234.3 51.9 0.22 18 693.8 0.16 35 10.5 0.27 5 LNU318 65067.2 53.1 0.2321 — — — — — — LNU318 65069.1 55.0 0.20 25 — — — — — — LNU296 65060.2 —— — 650.0 0.17 26 10.8 0.18 8 LNU296 65061.2 52.5 0.22 19 — — — — — —CONT. — 44.0 — — 514.6 — — 10.0 — — LNU507 64584.1 41.9 0.15 25 481.20.03 45 — — — LNU507 64585.2 — — — 400.9 0.25 21 12.1 0.21 4 LNU49465299.1 36.9 0.27 10 400.0 0.24 21 — — — LNU494 65300.2 44.4 0.27 32500.0 0.14 51 — — — LNU494 65302.1 44.4 0.02 32 425.0 0.13 28 — — —LNU494 65303.2 44.4 0.02 32 487.5 0.03 47 — — — LNU479 65497.3 43.8 0.0130 512.5 0.02 55 — — — LNU479 65497.5 — — — 556.2 0.27 68 — — — LNU47965499.1 — — — 531.2 0.02 60 12.4 0.05 7 LNU423 64596.1 — — — 481.2 0.2745 — — — LNU423 64598.3 36.9 0.27 10 — — — — — — LNU401 65492.4 — — — —— — 12.2 0.15 6 LNU401 65493.2 43.8 0.01 30 481.2 0.09 45 — — — LNU40165493.3 — — — 550.0 0.09 66 12.4 0.22 7 LNU401 65494.1 43.8 0.24 30500.0 0.20 51 — — — LNU401 65494.2 — — — 431.2 0.28 30 — — — LNU38865487.2 38.1 0.15 14 — — — — — — LNU388 65488.1 39.4 0.26 17 406.2 0.2123 — — — LNU388 65489.4 — — — — — — 12.8 0.07 11  LNU377 64604.6 40.00.06 19 406.2 0.27 23 — — — LNU377 64605.1 43.8 0.01 30 — — — 12.9 L 12 LNU348 64472.2 — — — 392.9 0.29 19 — — — LNU339 65058.2 — — — 406.2 0.2123 — — — LNU337 64955.2 — — — 412.5 0.21 25 — — — LNU336 64448.3 38.80.11 16 418.8 0.15 26 12.2 0.13 5 LNU336 64449.3 38.8 0.23 16 462.5 0.2840 12.0 0.21 4 LNU336 64449.4 40.6 0.19 21 — — — — — — LNU333 65295.238.8 0.11 16 437.5 0.10 32 — — — LNU333 65297.1 — — — — — — 12.9 L 12 LNU333 65297.2 43.1 0.03 29 456.2 0.06 38 — — — LNU324 64233.4 41.2 0.1123 500.0 0.03 51 — — — LNU318 65067.1 38.1 0.22 14 — — — 12.0 0.21 4LNU318 65067.2 — — — — — — 12.0 0.21 4 LNU304 64572.2 38.1 0.15 14 443.80.12 34 — — — LNU304 64575.2 — — — — — — 12.9 0.13 12  LNU296 65062.2 —— — 400.0 0.24 21 — — — LNU296 65064.1 — — — 450.0 0.12 36 12.4 0.22 7LNU292 64085.1 — — — 400.0 0.24 21 — — — CONT. — 33.5 — — 331.2 — — 11.6— — LNU519 64681.3 — — — 743.8 0.18 10 — — — LNU469 64311.5 — — — — — —10.4 0.15 7 LNU469 64311.8 77.5 0.11 25 — — — 10.1 0.19 4 LNU459 64541.4— — — — — — 10.1 0.23 3 LNU442 64060.2 — — — — — — 10.3 0.12 6 LNU42064008.4 74.4 0.04 20 — — — — — — LNU416 64137.1 69.4 0.17 12 — — — — — —LNU409 64687.2 — — — 806.2 0.11 20 — — — LNU409 64689.3 — — — 755.4 0.1912 10.5 0.11 8 LNU408 64248.10 — — — 737.5 0.21 10 — — — LNU363 64410.2— — — 750.0 0.15 11 — — — LNU314 64433.1 — — — — — — 10.4 0.03 7 CONT. —61.8 — — 673.2 — —  9.7 — — LNU514 64364.2 73.8 0.15 16 — — — — — —LNU509 64690.3 71.9 0.26 13 — — — — — — LNU397 64376.4 71.2 0.16 12800.0 0.18 13 — — — LNU369 64389.2 68.8 0.26  8 — — — — — — LNU34264036.2 77.5 0.21 22 — — — — — — CONT. — 63.6 — — 705.7 — — — — — LNU51363458.2 214.0  0.15 36 2432.1  0.19 40 — — — LNU512 63470.1 185.0  0.2517 — — —  9.9 0.08 7 LNU451 63496.2 190.6  0.06 21 2243.8  0.03 29 — — —LNU451 63499.1 — — — — — —  9.6 0.27 3 LNU451 63499.5 198.8  0.23 262306.2  0.25 33 10.0 0.03 8 LNU451 63500.1 — — — 2018.7  0.14 16 10.10.02 9 LNU424 63474.3 183.8  0.12 16 2181.2  0.17 25  9.9 0.03 7 LNU41563691.2 — — — — — —  9.6 0.27 3 LNU415 63692.2 — — — — — —  9.7 0.13 4LNU415 63693.4 — — — — — —  9.7 0.23 4 LNU411 63514.3 191.2  0.06 212250.0  0.02 29  9.6 0.27 3 LNU411 63515.3 173.8  0.28 10 1981.2  0.1614 — — — LNU375 63452.2 199.6  0.03 26 2375.0  L 37 10.1 0.20 9 LNU37563454.1 184.8  0.13 17 2086.6  0.07 20 — — — LNU375 63454.2 — — — — — — 9.7 0.13 4 LNU370 63544.3 179.4  0.20 14 2143.8  0.04 23 — — — LNU37063545.6 200.0  0.07 27 2306.2  0.08 33 — — — LNU370 63547.1 — — —1943.8  0.27 12 — — — LNU370 63548.2 — — — 1956.2  0.20 12 — — — LNU35763532.3 — — — — — —  9.9 0.03 7 LNU357 63533.1 210.6  0.01 33 2556.2 0.01 47 10.1 0.20 9 LNU357 63533.8 184.4  0.10 17 2100.0  0.06 21 — — —LNU357 63534.1 175.0  0.27 11 1993.8  0.22 15 — — — LNU356 63445.1 — — —1931.2  0.26 11 — — — LNU351 63466.1 — — — 2066.1  0.14 19 10.0 0.12 8LNU330 63439.1 — — — — — —  9.8 0.09 5 LNU326 63433.2 — — — — — —  9.60.27 3 LNU326 63433.4 186.2  0.09 18 2125.0  0.06 22 — — — LNU32663435.1 177.5  0.27 12 2056.2  0.21 18 — — — LNU319 63527.1 175.6  0.2411 2000.0  0.16 15 — — — LNU319 63528.1 183.1  0.26 16 — — — — — —LNU319 63530.1 — — — 1919.6  0.28 10 — — — LNU319 63530.3 192.5  0.07 222212.5  0.07 27 — — — LNU308 63414.1 185.6  0.09 18 2093.8  0.06 20 — —— LNU308 63414.4 173.8  0.30 10 2056.2  0.09 18 — — — LNU308 63415.3 — —— 2037.5  0.17 17 — — — LNU308 63417.5 184.4  0.30 17 — — — — — — LNU30863417.8 200.0  0.03 27 2300.0  0.02 32  9.9 0.08 7 LNU302 63378.3 — — —— — —  9.6 0.27 3 LNU302 63380.1 186.9  0.08 18 2181.2  0.03 25  9.60.27 3 LNU302 63381.1 190.6  0.06 21 2162.5  0.04 24 — — — LNU30263382.2 205.6  0.02 30 2462.5  L 42 — — — LNU291 63385.2 — — — 1937.5 0.24 11 — — — LNU291 63387.1 181.2  0.22 15 2112.5  0.12 21 10.1 0.20 9CONT. — 157.9  — — 1739.6  — —  9.3 — — LNU496 64194.2 — — — — — — 10.20.22 4 LNU496 64195.6 59.4 0.25  6 — — — — — — LNU482 64164.2 — — —762.5 0.22  8 — — — LNU477 63886.1 65.0 L 16 787.5 0.09 12 10.8 0.03 10LNU477 63888.1 60.0 0.10  7 — — — — — — LNU477 63889.5 — — — 768.8 0.19 9 10.2 0.22 4 LNU469 64311.8 — — — 843.8 0.24 20 10.9 0.11 12  LNU44464182.1 — — — — — — 10.6 0.06 8 LNU444 64182.3 60.6 0.16  9 — — — — — —LNU442 64056.1 — — — 818.8 0.15 16 10.6 L 8 LNU442 64060.2 60.6 0.03  9806.2 0.06 14 — — — LNU430 63935.1 61.9 0.26 11 850.0 0.13 21 10.3 0.025 LNU430 63936.1 60.0 0.10  7 — — — — — — LNU391 63980.6 59.4 0.08  6 —— — — — — LNU376 63987.3 70.6 0.26 26 918.8 0.04 30 11.0 L 12  LNU36664027.9 — — — — — — 10.2 0.06 4 LNU366 64028.3 — — — — — — 11.0 L 12 LNU363 64409.2 59.4 0.25  6 — — — — — — LNU353 64032.3 — — — 818.8 0.0816 — — — LNU314 64437.2 — — — — — — 10.5 0.02 7 CONT. — 55.8 — — 704.2 ——  9.8 — — LNU511 65036.2 72.5 0.23 23 900.0 0.20 23 — — — LNU51165037.1 72.5 0.02 23 1012.5  0.15 38 — — — LNU511 65037.3 65.6 0.10 12 —— — 11.2 0.20 4 LNU492 64174.1 72.5 0.08 23 — — — 11.4 L 6 LNU49264174.2 78.1 0.22 33 906.2 0.07 24 — — — LNU492 64175.1 66.2 0.23 13856.2 0.12 17 — — — LNU492 64176.4 78.8 0.09 34 993.8 L 36 — — — LNU47664041.2 68.1 0.03 16 931.2 0.02 27 — — — LNU476 64042.1 73.1 0.11 25925.0 0.16 26 — — — LNU476 64043.3 66.9 0.15 14 — — — 11.6 0.23 8 LNU47164838.1 63.8 0.17  9 — — — — — — LNU471 64838.3 80.6 0.05 37 950.0 0.0830 11.9 0.07 10 LNU471 64839.2 79.4 0.11 35 918.8 0.03 26 — — — LNU47164841.3 63.8 0.15  9 862.5 0.23 18 11.4 L 6 LNU463 64280.4 — — — 862.50.09 18 — — — LNU463 64281.3 79.4 0.16 35 987.5 L 35 — — — LNU46364282.12 66.4 0.21 13 — — — — — — LNU463 64283.4 65.6 0.20 12 — — — 11.40.01 6 LNU454 64796.2 — — — — — — 11.5 0.27 7 LNU454 64796.3 75.0 L 28881.2 0.06 20 — — — LNU454 64797.2 — — — — — — 11.6 L 7 LNU454 64800.571.2 0.03 21 818.8 0.25 12 — — — LNU422 64965.2 78.8 0.14 34 993.8 0.2636 — — — LNU422 64966.2 — — — 837.5 0.19 14 — — — LNU422 64969.1 66.90.06 14 — — — — — — LNU413 65019.1 — — — 968.8 0.09 32 11.4 0.03 6LNU413 65019.2 64.4 0.12 10 831.2 0.17 14 — — — LNU413 65021.4 79.4 0.0635 987.5 L 35 — — — LNU413 65021.5 69.4 0.02 18 862.5 0.12 18 — — —LNU413 65022.4 75.6 0.03 29 868.8 0.24 19 — — — LNU410 64971.1 — — —806.2 0.28 10 — — — LNU410 64971.2 — — — 912.5 0.17 25 — — — LNU41064974.3 — — — — — — 11.3 0.30 5 LNU387 64808.1 — — — 906.2 0.04 24 11.10.14 3 LNU387 64811.2 — — — — — — 11.2 0.05 4 LNU387 64811.3 66.9 0.0614 862.5 0.09 18 11.6 0.30 7 LNU382 64428.2 — — — 881.2 0.06 20 11.60.30 7 LNU382 64429.3 73.1 L 25 850.0 0.11 16 11.1 0.14 3 LNU382 64430.166.9 0.04 14 893.8 0.05 22 — — — LNU373 64826.4 — — — 837.5 0.16 14 11.50.27 7 LNU373 64827.2 73.1 0.11 25 893.8 0.04 22 — — — LNU373 64828.1 —— — 893.8 0.06 22 11.1 0.13 3 LNU373 64830.1 — — — 837.5 0.19 14 — — —LNU361 64832.1 82.5 0.07 40 1068.8  0.07 46 — — — LNU361 64836.2 83.8 L43 981.2 0.04 34 — — — LNU355 65012.1 68.1 0.03 16 818.8 0.25 12 — — —LNU355 65012.2 70.0 0.01 19 881.2 0.06 20 — — — LNU355 65014.2 — — —900.0 0.06 23 — — — LNU355 65015.2 80.0 L 36 956.2 0.01 31 11.4 L 6LNU332 64821.1 73.1 0.25 25 856.2 0.16 17 11.4 0.03 6 LNU332 64822.4 — —— — — — 11.6 L 8 LNU332 64824.3 — — — — — — 11.6 0.19 7 LNU332 64824.463.8 0.15  9 — — — 11.2 0.20 4 LNU307 64958.2 — — — — — — 11.3 0.02 5LNU307 64959.2 66.2 0.05 13 856.2 0.10 17 — — — LNU307 64960.2 — — —831.2 0.17 14 11.1 0.14 3 LNU303 65042.2 65.0 0.18 11 825.0 0.19 13 11.10.07 3 LNU303 65043.1 63.1 0.21  8 — — — — — — LNU303 65043.2 70.6 0.0720 875.0 0.07 20 11.6 0.11 8 LNU303 65046.1 68.1 0.04 16 912.5 0.03 2511.4 0.23 6 LNU303 65046.3 65.0 0.30 11 812.5 0.25 11 — — — LNU30065030.2 71.2 L 21 — — — — — — LNU300 65031.3 78.8 0.27 34 1012.5  L 38 —— — LNU300 65033.1 83.8 0.10 43 943.8 0.02 29 — — — LNU300 65033.3 — — —875.0 0.07 20 — — — CONT. — 58.7 — — 731.8 — — 10.8 — — LNU517 64297.9 —— — — — — 10.5 L 14  LNU514 64364.2 — — — — — —  9.8 0.13 6 LNU51464365.3 — — — — — —  9.8 0.13 6 LNU514 64366.1 — — — — — —  9.8 0.29 6LNU509 64690.3 — — — — — — 10.2 0.05 10  LNU509 64690.6 79.4 0.19  7 — —— — — — LNU509 64692.3 — — — — — — 10.4 0.17 12  LNU509 64692.6 — — — —— —  9.9 0.09 7 LNU504 64453.2 — — — — — —  9.8 0.06 5 LNU501 64196.183.8 0.06 13 — — —  9.7 0.19 5 LNU501 64197.1 82.8 0.20 11 — — —  9.9 L7 LNU501 64723.1 — — — — — — 10.1 L 9 LNU461 64666.1 — — — — — — 10.00.02 8 LNU461 64667.4 94.4 L 27 1106.2  0.01 25 — — — LNU461 64668.5 — —— — — — 10.3 0.23 11  LNU461 64669.3 83.1 0.02 12 — — —  9.6 0.30 3LNU397 64375.1 — — — — — — 10.1 0.01 9 LNU396 64315.13 91.2 0.01 23 — —— 10.1 0.19 9 LNU386 64393.1 80.0 0.21  8 — — —  9.4 0.25 2 LNU38664395.3 — — — — — —  9.9 0.09 7 LNU381 64286.4 87.5 0.17 18 956.2 0.24 8 — — — LNU372 64481.1 — — — — — — 10.2 0.05 10  LNU372 64483.3 — — — —— — 10.5 L 14  LNU372 64485.1 — — — — — — 10.0 0.02 8 LNU372 64485.383.8 0.27 13 — — — — — — LNU369 64386.1 81.2 0.05  9 — — — — — — LNU36964387.1 — — — — — — 10.1 0.12 9 LNU369 64389.2 87.9 L 18 — — — — — —LNU365 64711.2 92.5 0.22 24 — — — — — — LNU365 64712.3 — — — — — — 10.20.26 10  LNU350 64674.2 — — — 1025.0  0.14 15 — — — LNU350 64674.4 82.70.03 11 — — — — — — LNU350 64677.2 89.9 L 21 — — — 10.0 L 8 LNU34564333.4 81.2 0.06  9 — — — — — — LNU345 64337.1 — — — 1015.2  0.07 14 9.6 0.12 4 LNU342 64035.4 — — — — — — 10.1 0.01 9 LNU342 64036.2 81.90.29 10 — — — 10.8 0.05 16  LNU313 64663.2 — — — — — — 10.5 L 14  LNU31364664.1 — — — — — —  9.8 0.06 5 LNU294 64657.2 — — — — — —  9.5 0.25 3LNU294 64658.7 — — — — — —  9.6 0.30 3 CONT. — 74.4 — — 887.5 — —  9.2 —— LNU520 64156.14 — — — 681.2 0.10  8 — — — LNU481 64138.6 61.6 0.21 18— — — — — — LNU481 64141.1 — — — — — —  9.9 0.07 5 LNU477 63888.1 — — —— — —  9.6 0.20 2 LNU472 63920.6 — — — 756.2 0.21 20 — — — LNU47263949.8 67.5 0.21 29 — — — — — — LNU458 63893.1 — — — — — — 10.2 0.12 9LNU456 63991.8 — — — — — — 10.1 0.28 7 LNU439 64615.4 — — — 789.3 0.1825  9.8 0.03 4 LNU419 63896.2 — — — 775.0 0.27 23  9.9 0.07 5 LNU41963897.5 — — — 768.8 0.04 22  9.8 0.22 4 LNU407 64219.2 57.5 0.10 10 — —— 10.1 0.09 7 LNU393 63977.3 60.0 0.02 15 693.8 0.05 10 — — — LNU39363978.6 — — — 731.2 0.28 16  9.6 0.20 2 LNU374 63997.5 — — — — — — 10.40.10 10  LNU335 64169.2 — — — 875.0 0.26 39  9.8 0.22 4 CONT. — 52.4 — —629.2 — —  9.4 — — LNU520 64156.14 68.8 0.06 22 531.2 0.26 12  9.8 0.066 LNU518 64014.3 80.6 L 43 — — — — — — LNU518 64014.5 65.0 0.29 16 559.80.03 18  9.6 0.18 4 LNU518 64015.4 — — — — — —  9.9 0.06 6 LNU51864016.3 64.4 0.21 14 — — —  9.6 0.27 3 LNU518 64016.4 62.5 0.29 11 — — — 9.9 0.06 6 LNU500 64221.2 85.6 0.07 52 556.2 0.06 18 — — — LNU50064223.1 67.5 0.13 20 575.0 0.02 22  9.8 0.14 6 LNU500 64223.2 — — — — ——  9.7 0.13 4 LNU496 64193.3 68.8 0.05 22 — — — — — — LNU496 64194.2 — —— 518.8 0.17 10 — — — LNU493 64190.1 62.5 0.29 11 — — — — — — LNU49364190.3 — — — — — — 10.1 0.02 9 LNU493 64191.3 — — — 543.8 0.19 15 — — —LNU493 64191.4 — — — 543.8 0.19 15 — — — LNU481 64138.6 — — — 556.2 0.1518 — — — LNU481 64140.1 68.1 0.09 21 518.8 0.23 10  9.8 0.12 5 LNU48164141.1 — — — — — — 10.2 L 10  LNU458 63893.3 — — — — — —  9.8 0.12 5LNU419 63897.6 65.0 0.21 16 568.8 0.02 20 — — — LNU405 64158.9 — — — — ——  9.8 0.06 6 LNU405 64159.6 — — — — — —  9.9 0.04 6 LNU405 64159.8 73.80.29 31 556.2 0.03 18 — — — LNU343 64208.1 — — — 606.2 0.01 28 10.5 L13  LNU343 64208.2 — — — — — —  9.6 0.27 3 LNU343 64208.4 63.1 0.28 12612.5 0.23 30 — — — LNU340 64290.11 — — — 518.8 0.17 10 — — — LNU34064290.7 — — — 525.0 0.24 11  9.9 0.09 7 LNU340 64292.5 — — — — — —  9.80.06 6 LNU328 64150.1 68.1 0.14 21 581.2 0.01 23 10.1 0.02 9 LNU32864150.2 — — — 537.5 0.16 14 — — — LNU328 64150.4 — — — — — —  9.8 0.08 5LNU327 64487.2 — — — 618.8 0.30 31 — — — LNU327 64491.2 — — — 568.8 0.0220 — — — LNU322 63918.1 66.9 0.07 19 — — — — — — LNU312 64002.2 75.00.18 33 — — — 10.2 0.25 10  LNU312 64002.3 — — — — — —  9.7 0.13 4LNU306 64131.2 — — — 562.5 0.03 19 — — — LNU306 64132.1 — — — — — —  9.80.06 6 LNU305 64114.1 — — — — — —  9.8 0.12 5 CONT. — 56.2 — — 472.9 — — 9.3 — — LNU503 64203.1 — — — 1556.2  0.29 13 — — — LNU498 64185.3 — — —1568.8  0.05 14 — — — LNU482 64165.4 — — — 1468.8  0.17  7 — — — LNU44464182.3 153.8  0.07 23 1500.0  0.17  9 — — — LNU444 64183.1 156.9  L 251512.5  0.07 10 — — — LNU430 63934.3 — — — 1493.8  0.28  9 — — — LNU43063936.2 — — — 1525.0  0.28 11 — — — LNU393 63977.5 136.2  0.21  9 — — —— — — LNU393 63977.6 — — — 1450.0  0.25  6 — — — LNU385 64245.3 137.5 0.05 10 — — — — — — LNU366 64027.9 135.0  0.06  8 — — — 11.1 0.27 5LNU366 64028.3 138.1  0.06 10 1668.8  0.03 22 — — — LNU335 64168.1139.4  0.24 11 — — — — — — LNU317 64093.3 — — — — — — 11.2 0.27 6 CONT.— 125.5  — — 1372.5  — — 10.5 — — LNU462 63503.1 — — — — — — 11.7 0.08 6LNU462 63504.1 — — — 1950.0  0.20  6 — — — LNU455 64187.4 174.4  L 11 —— — — — — LNU455 64187.5 — — — 1987.5  L  8 11.9 0.01 8 LNU450 63712.3165.0  0.11  5 — — — 11.4 0.07 3 LNU448 63706.5 172.5  0.16 10 — — — — —— LNU425 63911.12 171.3  0.02 10 — — — 11.4 0.14 3 LNU425 63911.7 — — —2056.2  L 12 — — — LNU425 63911.9 176.2  0.12 13 1950.0  0.02  6 — — —LNU402 63913.4 — — — — — — 11.6 0.13 5 LNU402 63914.2 176.2  0.06 13 — —— — — — LNU399 63944.2 — — — — — — 11.6 0.30 5 LNU392 63696.2 — — — — —— 11.8 L 7 LNU390 63538.1 — — — — — — 11.8 L 7 LNU347 63510.4 — — — — —— 11.4 0.20 4 LNU329 63427.3 177.5  0.24 13 — — — — — — LNU329 63428.1165.6  0.15  6 — — — 11.4 0.05 4 LNU329 63428.2 — — — 2031.2  L 11 — — —LNU329 63430.3 — — — — — — 11.3 0.13 3 LNU323 63420.1 171.2  0.03  9 — —— 11.4 0.20 4 LNU323 63421.2 — — — — — — 11.5 0.07 4 LNU323 63424.1 — —— 1981.2  0.10  8 — — — LNU323 63424.4 176.9  0.01 13 — — — — — — CONT.— 156.5  — — 1835.4  — — 11.0 — — LNU511 65037.1 65.0 0.10 15 668.8 0.1411 — — — LNU511 65040.2 67.5 0.02 20 806.2 0.01 33 — — — LNU492 64174.172.0 L 27 703.6 0.21 16 — — — LNU492 64174.2 — — — — — — 10.9 0.29 4LNU492 64175.1 — — — 687.5 0.08 14 10.9 0.15 4 LNU492 64176.4 — — — — —— 10.8 0.29 3 LNU476 64041.2 — — — 687.5 0.12 14 — — — LNU471 64838.1 —— — 668.8 0.17 11 — — — LNU471 64838.3 66.9 0.04 18 668.8 0.14 11 — — —LNU471 64839.2 — — — 693.8 0.07 15 — — — LNU471 64841.3 61.2 0.24  8 — —— — — — LNU471 64842.1 74.4 0.23 32 793.8 0.22 31 — — — LNU463 64281.362.5 0.15 11 — — — — — — LNU463 64283.4 — — — 693.8 0.07 15 — — — LNU45464796.2 — — — 668.8 0.14 11 — — — LNU454 64797.2 76.9 L 36 818.8 L 36 —— — LNU454 64799.2 65.0 0.06 15 — — — 10.9 0.17 5 LNU454 64800.5 66.90.03 18 718.8 0.03 19 — — — LNU422 64966.1 — — — — — — 10.8 0.28 4LNU422 64969.1 66.9 0.27 18 — — — — — — LNU413 65021.4 75.0 0.29 33781.2 0.12 29 — — — LNU413 65022.4 67.5 0.05 20 700.0 0.05 16 11.3 0.048 LNU410 64973.2 66.2 0.04 17 706.2 0.05 17 — — — LNU387 64810.4 70.00.08 24 762.5 L 26 — — — LNU387 64811.2 63.1 0.23 12 693.8 0.06 15 — — —LNU382 64428.2 76.2 0.03 35 900.0 L 49 — — — LNU382 64432.1 62.5 0.22 11— — — — — — LNU373 64830.1 — — — 700.0 0.16 16 — — — LNU361 64834.1 — —— 706.2 0.27 17 11.1 0.05 6 LNU361 64835.2 — — — 662.5 0.24 10 — — —LNU361 64836.2 66.9 0.04 18 — — — — — — LNU355 65012.2 78.8 0.11 39825.0 0.20 37 — — — LNU355 65013.2 73.8 L 31 793.8 0.05 31 — — — LNU35565015.2 — — — 675.0 0.17 12 — — — LNU332 64821.1 69.4 0.27 23 712.5 0.2118 — — — LNU332 64822.4 68.8 0.02 22 706.2 0.05 17 10.8 0.29 3 LNU33264823.1 69.4 0.01 23 — — — 11.2 0.06 7 LNU332 64824.3 60.6 0.28  7 — — —— — — LNU307 64960.1 — — — 762.5 0.18 26 — — — LNU307 64962.2 68.1 0.0221 718.8 0.07 19 — — — LNU303 65043.1 — — — 712.5 0.03 18 — — — LNU30065033.3 60.6 0.28  7 — — — — — — CONT. — 56.5 — — 604.2 — — 10.4 — —LNU513 63458.2 — — — 1750.0  0.22  9 — — — LNU513 63458.3 141.2  0.26 101750.0  0.20  9 12.1 0.03 5 LNU513 63460.2 155.6  0.05 21 1868.8  0.2917 — — — LNU512 63470.1 156.2  0.04 22 1906.2  0.04 19 12.1 0.11 5LNU451 63497.5 158.8  0.03 24 2068.8  L 29 — — — LNU424 63476.3 — — —1831.2  0.14 14 — — — LNU415 63691.2 148.1  0.11 15 — — — — — — LNU37563452.2 140.6  0.29  9 — — — — — — LNU375 63454.1 146.2  0.29 14 1893.8 0.03 18 — — — LNU375 63454.2 148.8  0.10 16 1743.8  0.22  9 — — — LNU35763532.3 151.9  0.17 18 1875.0  0.04 17 — — — LNU357 63533.8 162.5  0.0226 2075.0  L 29 — — — LNU357 63534.4 158.8  0.14 24 2075.0  0.10 29 — —— LNU356 63444.1 146.2  0.25 14 1875.0  0.14 17 — — — LNU351 63466.1154.4  0.11 20 1918.8  0.03 20 — — — LNU344 63520.4 151.2  0.09 181800.0  0.11 12 — — — LNU344 63521.1 150.0  0.10 17 1781.2  0.15 11 — —— LNU330 63438.1 157.5  0.05 23 — — — — — — LNU330 63441.2 154.4  0.1720 1918.8  0.24 20 12.2 0.02 7 LNU326 63433.4 143.8  0.22 12 1775.0 0.15 11 — — — LNU326 63435.1 147.5  0.11 15 — — — — — — LNU319 63527.1156.9  0.04 22 1912.5  0.06 19 11.9 0.15 3 LNU319 63530.1 153.8  0.26 201893.8  0.05 18 12.1 0.02 6 LNU319 63530.3 165.0  0.01 28 2081.2  0.0230 — — — LNU302 63378.3 160.0  0.02 25 1912.5  0.03 19 — — — LNU29163385.1 158.8  0.05 24 2056.2  0.21 28 — — — LNU291 63385.2 153.8  0.2620 1937.5  0.15 21 — — — LNU291 63387.3 — — — 1975.0  0.27 23 — — —CONT. — 128.5 — — 1602.4  — — 11.5 — — Table 94. “CONT.”—Control;“Ave.”—Average; “% Incr.” = % increment; “p-val.”— p-value; L means thatp-value is less than 0.01, p < 0.1 was considered as significant.

TABLE 95 Genes showing improved plant biomass production at standardnitrogen growth conditions Rosette Diameter Plot Coverage [cm²] RosetteArea [cm²] [cm] Gene P- % % P- % Name Event # Ave. Val. Incr. Ave.P-Val. Incr. Ave. Val. Incr. LNU507 64584.2 68.4 0.16 18 8.6 0.16 18 5.10.07 12 LNU507 64585.2 67.0 0.21 16 8.4 0.21 16 5.1 0.05 13 LNU49465299.1 — — — — — — 4.8 0.26  6 LNU479 65497.5 75.3 0.04 30 9.4 0.04 305.2 0.03 15 LNU479 65499.1 76.5 0.23 32 9.6 0.23 32 5.3 0.09 17 LNU41865027.2 82.8 L 43 10.4  L 43 5.4 0.02 20 LNU401 65493.3 — — — — — — 5.00.29 11 LNU401 65494.1 78.3 0.18 35 9.8 0.18 35 5.3 0.11 17 LNU38865487.1 70.1 0.15 21 8.8 0.15 21 5.2 0.03 15 LNU377 64604.6 71.1 0.09 238.9 0.09 23 5.0 0.08 11 LNU368 64004.3 72.8 0.06 26 9.1 0.06 26 5.1 0.0713 LNU344 63521.1 80.1 0.06 39 10.0  0.06 39 5.4 0.06 19 LNU339 65055.166.0 0.25 14 8.2 0.25 14 4.9 0.17  8 LNU337 64955.2 71.3 0.08 23 8.90.08 23 5.1 0.05 13 LNU324 64234.3 72.8 0.17 26 9.1 0.17 26 5.2 0.17 14LNU318 65067.2 — — — — — — 4.9 0.22  7 LNU296 65060.2 71.1 0.09 23 8.90.09 23 5.1 0.05 13 CONT. — 57.8 — — 7.2 — — 4.5 — — LNU507 64584.1 92.50.08 18 11.6  0.08 18 5.8 0.13 11 LNU494 65300.2 98.9 0.14 26 12.4  0.1426 6.2 0.16 18 LNU494 65302.1 87.5 0.20 12 10.9  0.20 12 5.6 0.08  8LNU494 65303.2 98.3 0.15 25 12.3  0.15 25 5.9 0.11 12 LNU479 65497.3101.7  0.04 30 12.7  0.04 30 6.1 L 17 LNU479 65499.1 109.9  L 40 13.7  L40 6.3 L 20 LNU401 65493.2 91.7 0.20 17 11.5  0.20 17 5.9 0.15 12 LNU40165493.3 95.6 0.21 22 11.9  0.21 22 5.8 0.15 11 LNU401 65494.1 95.9 0.2022 12.0  0.20 22 5.9 0.17 13 LNU401 65494.2 — — — — — — 5.5 0.28  5LNU388 65488.1 — — — — — — 5.9 0.02 12 LNU377 64605.1 100.3  0.07 2812.5  0.07 28 6.1 0.02 17 LNU348 64472.2 — — — — — — 5.6 0.20  6 LNU33965058.2 86.5 0.30 10 10.8  0.30 10 — — — LNU336 64448.3 — — — — — — 5.70.06  9 LNU336 64449.4 — — — — — — 6.1 0.13 17 LNU333 65295.2 — — — — —— 5.7 0.07  8 LNU333 65297.2 88.3 0.17 13 11.0  0.17 13 5.8 0.03 12LNU324 64233.4 100.8  0.03 29 12.6  0.03 29 6.2 0.06 19 LNU304 64572.289.7 0.12 14 11.2  0.12 14 5.8 0.04 10 LNU304 64573.1 — — — — — — 5.60.15  7 LNU296 65062.2 — — — — — — 5.7 0.06  9 LNU292 64081.2 — — — — —— 5.7 0.23  9 LNU292 64084.1 — — — — — — 5.6 0.25  6 LNU292 64085.4 — —— — — — 5.6 0.19  7 CONT. — 78.4 — — 9.8 — — 5.2 — — LNU469 64308.5 59.70.22 12 7.5 0.22 12 4.8 0.09  6 LNU469 64311.8 60.7 0.23 14 7.6 0.23 14— — — LNU459 64541.4 58.8 0.17 10 7.3 0.17 10 — — — LNU442 64056.1 — — —— — — 4.7 0.14  5 LNU442 64553.1 — — — — — — 4.7 0.17  5 LNU409 64687.262.3 0.04 17 7.8 0.04 17 5.0 0.01 11 LNU363 64410.2 66.6 0.13 25 8.30.13 25 5.1 0.13 13 LNU314 64433.1 61.6 0.11 15 7.7 0.11 15 4.8 0.14  7LNU314 64433.3 — — — — — — 4.7 0.17  5 CONT. — 53.4 — — 6.7 — — 4.5 — —LNU517 64296.4 53.2 0.12 20 6.6 0.12 20 4.5 0.15  7 LNU501 64197.10 51.60.09 16 6.5 0.09 16 4.4 0.19  5 LNU397 64376.4 49.7 0.12 12 6.2 0.12 124.4 0.27  4 LNU369 64387.2 48.1 0.25  8 6.0 0.25  8 — — — LNU369 64389.2— — — — — — 4.6 0.24 10 LNU342 64036.2 52.9 0.03 19 6.6 0.03 19 4.7 0.0211 CONT. — 44.4 — — 5.5 — — 4.2 — — LNU513 63458.2 49.5 L 35 6.2 L 354.4 L 15 LNU512 63468.3 40.3 0.10 10 5.0 0.10 10 4.0 0.19  4 LNU51263470.1 43.2 0.04 17 5.4 0.04 17 4.1 0.26  7 LNU451 63496.2 39.8 0.14  85.0 0.14  8 4.0 0.12  5 LNU451 63499.1 41.9 0.04 14 5.2 0.04 14 — — —LNU451 63499.5 46.0 0.08 25 5.8 0.08 25 4.2 0.10 10 LNU451 63500.1 44.30.05 20 5.5 0.05 20 — — — LNU424 63474.3 41.7 0.21 13 5.2 0.21 13 — — —LNU424 63476.3 40.4 0.09 10 5.1 0.09 10 — — — LNU415 63692.3 39.5 0.22 7 4.9 0.22  7 — — — LNU415 63693.4 43.2 0.12 17 5.4 0.12 17 4.1 0.17  6LNU411 63514.3 44.8 L 22 5.6 L 22 4.1 0.04  7 LNU411 63515.3 42.0 0.0314 5.2 0.03 14 4.1 0.05  7 LNU411 63518.1 39.5 0.17  7 4.9 0.17  7 — — —LNU375 63452.2 41.2 0.05 12 5.1 0.05 12 4.0 0.21  5 LNU375 63454.1 45.60.02 24 5.7 0.02 24 4.2 0.02 11 LNU375 63454.2 46.8 0.21 27 5.8 0.21 274.4 0.24 16 LNU370 63545.6 45.0 0.16 22 5.6 0.16 22 4.3 0.08 11 LNU37063547.1 39.0 0.27  6 4.9 0.27  6 — — — LNU370 63548.2 40.8 0.07 11 5.10.07 11 — — — LNU357 63532.3 39.6 0.17  8 5.0 0.17  8 — — — LNU35763533.1 52.9 0.07 44 6.6 0.07 44 4.5 0.05 18 LNU357 63533.8 44.2 L 205.5 L 20 4.1 0.10  8 LNU357 63534.1 42.8 0.23 16 5.3 0.23 16 — — —LNU356 63444.2 39.3 0.20  7 4.9 0.20  7 — — — LNU356 63445.1 39.6 0.16 8 4.9 0.16  8 — — — LNU351 63462.1 38.8 0.29  5 4.8 0.29  5 — — —LNU351 63464.1 41.1 0.09 12 5.1 0.09 12 4.1 0.04  7 LNU330 63438.1 40.80.06 11 5.1 0.06 11 4.0 0.14  5 LNU326 63433.4 44.0 L 20 5.5 L 20 4.20.05 10 LNU326 63435.1 43.2 0.11 18 5.4 0.11 18 4.1 0.08  6 LNU31963527.1 39.7 0.14  8 5.0 0.14  8 — — — LNU319 63528.1 — — — — — — 4.20.24  9 LNU319 63530.3 45.9 L 25 5.7 L 25 4.3 L 12 LNU308 63414.1 43.20.16 18 5.4 0.16 18 4.1 0.20  8 LNU308 63414.4 40.4 0.21 10 5.1 0.21 104.0 0.24  4 LNU308 63415.3 43.6 0.01 19 5.5 0.01 19 4.1 0.04  7 LNU30863417.5 — — — — — — 4.1 0.17  9 LNU308 63417.8 49.3 L 34 6.2 L 34 4.4 L14 LNU302 63378.3 39.6 0.16  8 5.0 0.16  8 3.9 0.30  3 LNU302 63380.145.0 L 22 5.6 L 22 4.1 0.06  7 LNU302 63381.1 42.4 0.02 15 5.3 0.02 154.0 0.11  5 LNU302 63382.2 48.8 L 33 6.1 L 33 4.4 L 14 LNU291 63385.239.3 0.20  7 4.9 0.20  7 — — — LNU291 63387.1 45.8 L 24 5.7 L 24 4.20.05 11 CONT. — 36.8 — — 4.6 — — 3.8 — — LNU482 64164.8 — — — — — — 5.10.20  4 LNU477 63886.1 — — — — — — 5.1 0.30  3 LNU477 63888.1 — — — — —— 5.4 0.30 10 LNU477 63889.5 71.1 0.05 14 8.9 0.05 14 5.1 0.18  4 LNU46964311.8 75.0 0.28 20 9.4 0.28 20 — — — LNU444 64182.1 70.5 0.16 13 8.80.16 13 5.2 0.22  5 LNU442 64056.1 68.3 0.13  9 8.5 0.13  9 5.1 0.18  4LNU442 64060.2 — — — 8.8 0.06 13 5.3 0.02  9 LNU376 63987.3 81.3 L 3010.2  L 30 5.6 0.02 13 LNU363 64410.2 68.1 0.18  9 8.5 0.18  9 5.2 0.06 6 CONT. — 62.5 — — 7.8 — — 4.9 — — LNU511 65036.2 114.4  L 36 14.3  L36 6.4 0.04 14 LNU511 65037.1 121.5  0.06 45 15.2  0.06 45 6.6 0.04 17LNU492 64174.2 102.7  0.18 22 12.8  0.18 22 6.0 0.13  7 LNU492 64175.193.8 0.20 12 11.7  0.20 12 — — — LNU492 64176.4 112.0  0.03 34 14.0 0.03 34 6.4 L 13 LNU476 64041.2 103.5  0.22 23 12.9  0.22 23 6.3 0.18 13LNU476 64042.1 104.4  L 24 13.0  L 24 6.3 0.01 12 LNU476 64043.3 90.40.29  8 11.3  0.29  8 — — — LNU471 64838.3 111.2  0.04 33 13.9  0.04 336.4 0.20 13 LNU471 64839.2 105.0  0.01 25 13.1  0.01 25 6.3 0.01 11LNU471 64841.3 99.8 0.05 19 12.5  0.05 19 — — — LNU471 64842.1 106.7  L27 13.3  L 27 6.2 0.01 11 LNU463 64280.4 99.3 0.06 18 12.4  0.06 18 5.90.13  5 LNU463 64281.3 119.8  0.03 43 15.0  0.03 43 6.7 0.05 19 LNU46364283.4 93.7 0.14 12 11.7  0.14 12 5.9 0.23  4 LNU454 64796.3 99.4 0.0319 12.4  0.03 19 6.1 0.03  9 LNU454 64797.2 93.3 0.24 11 11.7  0.24 11 —— — LNU422 64966.2 — — — — — — 5.8 0.28  4 LNU422 64969.1 94.1 0.30 1211.8  0.30 12 — — — LNU413 65019.1 119.6  0.06 43 14.9  0.06 43 6.8 0.1720 LNU413 65019.2 101.4  0.02 21 12.7  0.02 21 6.1 0.03  8 LNU41365021.4 108.3  L 29 13.5  L 29 6.6 0.07 17 LNU413 65021.5 101.7  0.02 2112.7  0.02 21 6.2 0.01 11 LNU413 65022.4 97.1 0.26 16 12.1  0.26 16 — —— LNU410 64974.3 101.6  0.22 21 12.7  0.22 21 — — — LNU387 64808.1 97.80.04 17 12.2  0.04 17 6.0 0.10  6 LNU387 64810.4 — — — — — — 5.8 0.25  4LNU387 64811.3 104.9  L 25 13.1  L 25 6.3 L 13 LNU382 64428.2 97.9 0.0917 12.2  0.09 17 6.1 0.22  8 LNU382 64429.3 96.3 0.07 15 12.0  0.07 155.9 0.23  5 LNU382 64430.1 104.8  L 25 13.1  L 25 6.4 L 13 LNU37364827.2 104.7  L 25 13.1  L 25 6.1 0.04  8 LNU373 64828.1 103.3  0.01 2312.9  0.01 23 6.3 L 12 LNU373 64830.3 91.4 0.27  9 11.4  0.27  9 — — —LNU361 64832.1 139.6  L 66 17.5  L 66 7.3 L 29 LNU361 64836.2 107.0 0.26 28 13.4  0.26 28 6.3 0.23 11 LNU355 65012.1 100.2  0.03 19 12.5 0.03 19 6.2 0.02 10 LNU355 65012.2 93.8 0.11 12 11.7  0.11 12 6.0 0.07 7 LNU355 65014.2 97.4 0.25 16 12.2  0.25 16 — — — LNU355 65015.2 105.9 0.20 26 13.2  0.20 26 6.3 0.07 12 LNU332 64821.1 102.3  0.02 22 12.8 0.02 22 6.1 0.04  9 LNU307 64959.2 97.2 0.05 16 12.1  0.05 16 6.0 0.08 7 LNU307 64960.2 97.0 0.09 16 12.1  0.09 16 6.0 0.12  6 LNU303 65042.292.8 0.16 11 11.6  0.16 11 6.0 0.20  6 LNU303 65043.2 100.2  0.09 1912.5  0.09 19 6.1 0.05  9 LNU303 65046.1 114.4  0.04 36 14.3  0.04 366.3 0.12 12 LNU300 65030.2 102.4  0.02 22 12.8  0.02 22 6.3 0.18 11LNU300 65031.3 115.7  0.02 38 14.5  0.02 38 6.6 0.14 17 LNU300 65033.1111.8  L 33 14.0  L 33 6.4 0.07 14 CONT. — 83.9 — — 10.5  — — 5.6 — —LNU517 64297.9 66.5 0.02 27 8.3 0.02 27 4.9 0.28  6 LNU514 64364.2 61.00.12 16 7.6 0.12 16 — — — LNU514 64366.1 59.3 0.21 13 7.4 0.21 13 — — —LNU509 64690.3 73.2 L 39 9.2 L 39 5.3 0.03 14 LNU509 64692.3 70.0 L 338.7 L 33 5.1 0.08 10 LNU509 64692.6 68.2 0.02 30 8.5 0.02 30 5.2 0.04 12LNU501 64197.1 62.4 0.07 19 7.8 0.07 19 — — — LNU461 64666.1 64.1 0.1222 8.0 0.12 22 — — — LNU461 64667.4 61.4 0.08 17 7.7 0.08 17 4.9 0.19  7LNU461 64668.5 67.3 0.23 28 8.4 0.23 28 — — — LNU397 64374.1 59.9 0.2014 7.5 0.20 14 — — — LNU397 64375.1 66.5 0.10 27 8.3 0.10 27 5.0 0.23  9LNU397 64376.4 59.2 0.18 13 7.4 0.18 13 — — — LNU396 64315.13 74.9 L 429.4 L 42 5.5 L 18 LNU372 64481.1 66.0 0.02 26 8.2 0.02 26 5.0 0.21  9LNU372 64483.3 70.4 0.07 34 8.8 0.07 34 5.1 0.10 10 LNU372 64485.1 62.80.05 19 7.8 0.05 19 — — — LNU372 64485.3 61.9 0.07 18 7.7 0.07 18 5.10.08 11 LNU369 64386.1 65.0 0.05 24 8.1 0.05 24 5.0 0.20  8 LNU36964387.1 63.7 0.06 21 8.0 0.06 21 — — — LNU369 64389.2 62.7 0.28 19 7.80.28 19 — — — LNU365 64711.2 58.4 0.21 11 7.3 0.21 11 — — — LNU36564712.3 63.7 0.06 21 8.0 0.06 21 — — — LNU350 64674.2 68.9 L 31 8.6 L 315.3 0.03 14 LNU345 64337.1 63.9 0.06 22 8.0 0.06 22 5.1 0.17 10 LNU34264035.4 59.9 0.14 14 7.5 0.14 14 — — — LNU342 64036.2 66.0 0.02 26 8.30.02 26 5.0 0.13  8 LNU313 64661.8 66.7 0.04 27 8.3 0.04 27 5.1 0.16 10LNU313 64663.2 70.9 L 35 8.9 L 35 5.1 0.07 11 LNU313 64664.1 61.5 0.2017 7.7 0.20 17 4.9 0.26  6 LNU294 64657.2 64.3 0.09 22 8.0 0.09 22 5.10.26 11 CONT. — 52.6 — — 6.6 — — 4.6 — — LNU477 63889.4 — — — — — — 5.00.08  4 LNU439 64615.4 64.0 0.03 10 8.0 0.03 10 — — — LNU425 63911.1163.4 0.26  9 7.9 0.26  9 — — — LNU419 63896.2 68.2 L 17 8.5 L 17 5.2 L 7 LNU419 63897.5 — — — — — — 5.0 0.06  4 CONT. — 58.3 — — 7.3 — — 4.8 —— LNU520 64156.7 60.1 0.05 16 7.5 0.05 16 4.8 0.15  9 LNU518 64014.556.5 0.23  9 7.1 0.23  9 4.6 0.18  5 LNU518 64016.4 — — — — — — 4.6 0.23 4 LNU493 64190.3 57.2 0.24 10 7.1 0.24 10 — — — LNU493 64191.3 60.50.03 16 7.6 0.03 16 4.7 0.04  7 LNU472 63920.6 57.7 0.11 11 7.2 0.11 114.7 0.16  7 LNU419 63896.2 — — — — — — 4.6 0.22  4 LNU419 63897.5 — — —— — — 4.8 0.03  8 LNU419 63897.6 64.8 0.16 25 8.1 0.16 25 4.9 0.07 11LNU343 64208.1 69.9 L 34 8.7 L 34 5.0 L 14 LNU343 64208.2 56.3 0.20  87.0 0.20  8 4.6 0.14  6 LNU343 64209.2 — — — — — — 4.5 0.28  3 LNU34064290.7 56.3 0.20  8 7.0 0.20  8 4.7 0.08  8 LNU340 64291.10 55.4 0.29 76.9 0.29  7 — — — LNU328 64150.1 63.5 0.15 22 7.9 0.15 22 5.1 0.18 15LNU328 64150.2 — — — — — — 4.6 0.14  5 LNU327 64491.2 57.2 0.14 10 7.10.14 10 4.6 0.21  4 LNU312 64002.2 59.3 0.17 14 7.4 0.17 14 4.7 0.16  6CONT. — 52.0 — — 6.5 — — 4.4 — — LNU503 64203.1 75.8 0.08 19 9.5 0.08 195.3 0.04  9 LNU498 64185.3 69.7 0.26  9 8.7 0.26  9 — — — LNU430 63936.2— — — — — — 5.3 0.17  7 LNU366 64028.3 76.3 0.28 20 9.5 0.28 20 5.2 0.27 6 CONT. — 63.7 — — 8.0 — — 4.9 — — LNU462 63504.1 88.9 0.28  5 11.1 0.28 5 — — — LNU455 64187.5 97.8 0.02 16 12.2  0.02 16 6.1 0.29  8LNU425 63911.7 92.0 0.03  9 11.5  0.03  9 5.9 0.06  5 LNU392 63698.2 — —— — — — 5.8 0.02  3 LNU390 63539.4 — — — — — — 5.8 0.08  3 LNU32963428.2 94.4 0.05 12 11.8  0.05 12 5.8 0.06  3 LNU323 63424.1 96.1 L 1412.0  L 14 6.0 0.27  7 CONT. — 84.6 — — 10.6  — — 5.6 — — LNU511 65037.1— — — — — — 5.1 0.30  4 LNU511 65040.2 77.4 0.02 18 9.7 0.02 18 5.4 0.0111 LNU492 64175.1 71.8 0.15 10 9.0 0.15 10 5.2 0.15  6 LNU471 64839.276.5 0.01 17 9.6 0.01 17 5.4 0.03 11 LNU471 64842.1 84.3 L 29 10.5  L 295.6 L 16 LNU463 64283.4 79.2 0.01 21 9.9 0.01 21 5.6 L 14 LNU454 64797.278.1 0.02 19 9.8 0.02 19 5.3 0.02  9 LNU454 64799.2 78.1 L 19 9.8 L 195.3 0.03  9 LNU454 64800.5 — — — — — — 5.1 0.25  4 LNU413 65021.4 73.80.11 13 9.2 0.11 13 5.3 0.04  8 LNU413 65022.4 91.2 0.05 39 11.4  0.0539 5.7 L 17 LNU410 64971.1 — — — — — — 5.2 0.21  7 LNU387 64810.4 70.70.15  8 8.8 0.15  8 5.2 0.06  7 LNU387 64811.3 — — — — — — 5.1 0.15  5LNU382 64428.2 82.4 L 26 10.3  L 26 5.6 L 14 LNU373 64830.1 — — — — — —5.2 0.29  6 LNU361 64834.1 77.5 0.01 18 9.7 0.01 18 5.3 0.03  9 LNU35565012.2 86.3 0.05 32 10.8  0.05 32 5.7 0.03 16 LNU355 65013.2 — — — — —— 5.4 0.29 10 LNU355 65014.2 79.6 L 21 9.9 L 21 5.3 0.03  9 LNU33264822.4 70.9 0.15  8 8.9 0.15  8 5.1 0.18  4 LNU307 64960.1 — — — — — —5.3 0.13  9 CONT. — 65.5 — — 8.2 — — 4.9 — — LNU513 63458.3 72.5 0.22  99.1 0.22  9 — — — LNU512 63468.3 72.3 0.24  9 9.0 0.24  9 5.2 0.27  5LNU451 63497.5 81.4 0.04 23 10.2  0.04 23 5.5 0.13 10 LNU424 63476.374.4 0.13 12 9.3 0.13 12 — — — LNU415 63691.2 73.2 0.25 10 9.2 0.25 105.2 0.27  5 LNU357 63532.3 85.0 0.02 28 10.6  0.02 28 5.6 0.02 13 LNU35763533.8 85.9 L 30 10.7  L 30 5.7 0.01 15 LNU357 63534.4 80.1 0.09 2110.0  0.09 21 5.5 0.26 11 LNU351 63466.1 77.5 0.26 17 9.7 0.26 17 — — —LNU344 63520.4 74.9 0.11 13 9.4 0.11 13 5.4 0.18  9 LNU344 63521.1 76.10.08 15 9.5 0.08 15 — — — LNU330 63438.1 83.6 0.30 26 10.4  0.30 26 5.50.25 11 LNU330 63441.2 76.0 0.08 15 9.5 0.08 15 5.3 0.18  7 LNU32663433.2 — — — — — — 5.3 0.30  8 LNU319 63527.1 73.7 0.16 11 9.2 0.16 11— — — LNU319 63530.1 78.6 0.18 19 9.8 0.18 19 — — — LNU319 63530.3 79.20.06 19 9.9 0.06 19 — — — LNU302 63378.3 86.6 L 31 10.8  L 31 5.7 0.0115 LNU291 63385.1 81.5 0.03 23 10.2  0.03 23 5.6 0.11 13 LNU291 63387.382.6 0.16 25 10.3  0.16 25 5.5 0.16 11 CONT. — 66.3 — — 8.3 — — 5.0 — —Table 95: “CONT.”—Control; “Ave.”—Average; “% Incr.” = % increment;“p-val.”— p-value; L means that p-value is less than 0.01, p < 0.1 wasconsidered as significant.

The genes listed in Table 96 improved plant NUE when grown at standardnitrogen concentration levels. These genes produced faster developingplants when grown under limiting nitrogen growth conditions, compared tocontrol plants as measured by growth rate of leaf number, rosettediameter and plot coverage.

TABLE 96 RGR Of Leaf RGR Of Plot RGR Of Rosette Number Coverage DiameterGene P- % P- % P- % Name Event # Ave. Val. Incr. Ave. Val. Incr. Ave.Val. Incr. LNU507 64087.1 — — — — — — 0.5 0.30 14 LNU507 64584.2 — — —8.9 0.29 21 0.5 0.15 20 LNU507 64585.2 — — — — — — 0.5 0.08 24 LNU47965497.5 — — — 9.9 0.09 34 0.5 0.13 21 LNU479 65499.1 — — — 9.9 0.10 340.5 0.16 19 LNU418 65027.2 — — — 10.8  0.03 45 0.5 0.12 21 LNU40165493.3 — — — 8.9 0.30 21 0.5 0.11 23 LNU401 65494.1 — — — 10.2  0.07 380.5 0.10 23 LNU388 65487.1 — — — 9.1 0.24 23 0.5 0.10 23 LNU377 64604.6— — — 9.4 0.18 27 0.5 0.16 19 LNU368 64004.3 — — — 9.5 0.14 29 0.5 0.1818 LNU344 63521.1 0.9 0.19 22 10.6  0.04 43 0.5 0.04 29 LNU339 65055.1 —— — — — — 0.5 0.18 18 LNU337 64952.1 — — — — — — 0.5 0.13 22 LNU33764953.1 0.9 0.23 21 — — — — — — LNU337 64954.1 0.9 0.22 20 — — — — — —LNU337 64955.2 — — — 9.4 0.18 26 0.5 0.10 23 LNU324 64234.3 — — — 9.50.16 28 0.5 0.22 17 LNU318 65067.1 — — — — — — 0.5 0.27 15 LNU31865067.2 — — — — — — 0.5 0.19 17 LNU296 65060.2 — — — 9.2 0.21 24 0.50.22 16 CONT. — 0.7 — — 7.4 — — 0.4 — — LNU507 64584.1 — — — 11.1  0.2022 0.5 0.10 22 LNU507 64585.2 — — — — — — 0.5 0.24 16 LNU494 65300.2 — —— 11.9  0.08 30 0.5 0.05 28 LNU494 65303.2 — — — 11.4  0.14 25 — — —LNU479 65497.3 — — — 12.3  0.04 34 0.5 0.07 24 LNU479 65497.5 — — —12.6  0.06 38 0.5 0.08 28 LNU479 65498.3 0.7 0.23 20 — — — — — — LNU47965499.1 — — — 13.3  0.01 45 0.5 0.05 27 LNU423 64596.1 — — — 11.2  0.2422 — — — LNU418 65027.2 0.7 0.20 20 — — — — — — LNU401 65493.2 — — —10.8  0.29 18 0.5 0.22 16 LNU401 65493.3 — — — 11.4  0.15 25 0.5 0.21 17LNU401 65494.1 0.7 0.18 19 11.6  0.11 27 0.5 0.12 21 LNU401 65494.2 0.80.07 28 — — — — — — LNU388 65488.1 0.7 0.19 20 — — — 0.5 0.12 21 LNU38865489.4 0.8 0.01 37 — — — — — — LNU377 64602.2 0.8 0.14 23 — — — — — —LNU377 64604.3 0.7 0.21 19 — — — — — — LNU377 64605.1 0.8 0.15 23 11.9 0.08 29 0.5 0.18 18 LNU348 64472.2 — — — — — — 0.5 0.28 14 LNU33965055.1 0.8 0.10 26 — — — — — — LNU339 65058.2 — — — — — — 0.5 0.21 17LNU337 64955.2 — — — 10.9  0.28 19 — — — LNU336 64448.3 0.7 0.22 19 — —— 0.5 0.23 16 LNU336 64449.3 — — — — — — 0.5 0.12 22 LNU336 64449.4 — —— — — — 0.5 0.21 17 LNU333 65295.2 — — — — — — 0.5 0.23 16 LNU33365297.1 0.8 0.03 36 — — — — — — LNU333 65297.2 — — — — — — 0.5 0.15 19LNU324 64233.4 — — — 12.2  0.05 33 0.5 0.05 28 LNU324 64234.4 0.8 0.1426 — — — — — — LNU318 65066.6 — — — — — — 0.5 0.26 15 LNU304 64572.2 — —— — — — 0.5 0.17 18 LNU304 64573.1 — — — — — — 0.5 0.19 17 LNU30464575.2 0.8 0.04 35 — — — — — — LNU296 65062.2 — — — — — — 0.5 0.16 18LNU292 64081.2 — — — — — — 0.5 0.27 15 LNU292 64084.1 — — — — — — 0.50.24 16 LNU292 64085.1 0.7 0.22 19 — — — 0.5 0.26 15 LNU292 64085.4 0.70.20 20 — — — — — — CONT. — 0.6 — — 9.2 — — 0.4 — — LNU508 64459.5 0.70.22 23 — — — — — — LNU469 64308.4 — — — 8.4 0.19 23 — — — LNU46964311.5 0.7 0.21 22 — — — — — — LNU442 64060.2 0.7 0.22 22 — — — — — —LNU409 64687.2 — — — 8.0 0.25 17 0.5 0.14 16 LNU409 64689.3 0.7 0.26 20— — — — — — LNU363 64410.2 — — — 8.6 0.11 26 0.4 0.24 13 LNU314 64433.1— — — 7.9 0.29 16 — — — CONT. — 0.6 — — 6.8 — — 0.4 — — LNU517 64296.4 —— — 6.7 0.17 21 — — — LNU501 64197.10 — — — 6.4 0.29 16 — — — LNU39764373.3 — — — 6.7 0.19 21 0.4 0.21 15 LNU369 64387.1 0.6 0.29 18 — — — —— — LNU369 64389.2 — — — 6.7 0.19 21 — — — LNU342 64036.2 — — — 6.7 0.1721 0.4 0.21 15 CONT. — 0.5 — — 5.5 — — 0.4 — — LNU513 63458.2 — — — 6.30.03 34 0.4 0.06 14 LNU512 63470.1 — — — 5.5 0.24 17 — — — LNU45163496.2 — — — — — — 0.4 0.13 11 LNU451 63499.5 — — — 5.9 0.10 25 0.40.17 10 LNU451 63500.1 — — — 5.7 0.20 20 — — — LNU415 63693.4 — — — 5.60.25 17 — — — LNU411 63514.3 — — — 5.7 0.16 21 — — — LNU375 63452.2 0.70.22 17 — — — 0.4 0.30  8 LNU375 63454.1 — — — 5.9 0.12 24 0.4 0.08 13LNU375 63454.2 — — — 6.0 0.09 27 0.4 0.10 14 LNU370 63545.2 — — — — — —0.4 0.13 12 LNU370 63545.6 — — — 5.8 0.13 23 0.4 0.08 13 LNU357 63533.1— — — 6.8 0.01 43 0.4 0.06 15 LNU357 63533.8 — — — 5.7 0.17 21 — — —LNU357 63534.1 — — — 5.5 0.29 16 0.4 0.28  8 LNU351 63464.1 — — — — — —0.4 0.29  8 LNU326 63433.4 — — — 5.7 0.18 20 0.4 0.09 13 LNU326 63435.1— — — 5.6 0.24 18 — — — LNU319 63528.1 — — — 5.8 0.16 22 — — — LNU31963530.3 — — — 5.9 0.11 24 0.4 0.11 12 LNU308 63414.1 — — — 5.6 0.25 180.4 0.18 10 LNU308 63415.3 — — — 5.6 0.21 19 — — — LNU308 63417.5 — — —5.6 0.22 19 0.4 0.17 10 LNU308 63417.8 — — — 6.4 0.03 35 0.4 0.02 16LNU302 63380.1 — — — 5.8 0.15 22 0.4 0.18 10 LNU302 63381.1 — — — 5.50.27 16 0.4 0.22  9 LNU302 63382.2 — — — 6.3 0.04 33 0.4 0.05 15 LNU29163385.1 0.7 0.29 16 — — — — — — LNU291 63387.1 0.7 0.28 15 5.9 0.11 250.4 0.20  9 CONT. — 0.6 — — 4.7 — — 0.3 — — LNU477 63888.1 — — — 9.70.19 21 — — — LNU469 64311.8 — — — 9.4 0.26 17 — — — LNU442 64056.1 0.70.26 15 — — — — — — LNU376 63987.3 0.7 0.26 14 10.5  0.06 30 0.5 0.13 15LNU314 64433.1 0.8 0.21 19 — — — — — — CONT. — 0.6 — — 8.0 — — 0.4 — —LNU511 65036.2 — — — 14.1  0.01 37 0.6 0.23 11 LNU511 65037.1 — — —14.8  L 44 — — — LNU511 65037.3 — — — 12.1  0.27 18 — — — LNU492 64174.2— — — 12.8  0.11 24 — — — LNU492 64176.4 — — — 13.9  0.03 35 0.6 0.14 15LNU476 64041.2 — — — 12.8  0.12 24 0.6 0.12 16 LNU476 64042.1 — — —13.0  0.08 27 0.6 0.16 14 LNU471 64838.3 — — — 13.8  0.03 34 — — —LNU471 64839.2 — — — 12.9  0.08 26 — — — LNU471 64841.3 — — — 12.5  0.1621 — — — LNU471 64842.1 — — — 13.1  0.06 28 — — — LNU463 64280.4 — — —12.2  0.21 19 — — — LNU463 64281.3 — — — 14.8  L 44 0.6 0.07 18 LNU45464796.3 — — — 12.0  0.24 17 — — — LNU454 64797.2 0.8 0.23 20 — — — — — —LNU422 64965.2 — — — 13.8  0.05 34 0.6 0.13 17 LNU413 65019.1 — — —14.8  L 43 0.6 0.08 19 LNU413 65019.2 — — — 12.1  0.23 18 — — — LNU41365021.4 — — — 13.3  0.05 29 0.6 0.12 15 LNU413 65021.5 — — — 12.5  0.1421 — — — LNU410 64971.2 — — — 12.8  0.16 24 0.6 0.27 12 LNU410 64974.3 —— — 12.5  0.16 22 — — — LNU387 64808.1 — — — 11.9  0.29 16 — — — LNU38764811.3 — — — 12.8  0.10 25 0.6 0.22 12 LNU382 64428.2 — — — 12.0  0.2716 — — — LNU382 64430.1 — — — 12.8  0.11 24 — — — LNU373 64826.4 — — —12.1  0.25 18 — — — LNU373 64827.2 — — — 12.8  0.10 25 — — — LNU37364828.1 — — — 12.7  0.12 23 0.6 0.25 11 LNU361 64832.1 — — — 17.5  L 700.7 L 30 LNU361 64836.2 — — — 13.2  0.08 28 — — — LNU355 65012.1 — — —12.2  0.21 19 — — — LNU355 65014.2 — — — 12.1  0.25 18 0.6 0.29 11LNU355 65015.2 — — — 13.3  0.06 29 0.6 0.17 13 LNU332 64821.1 — — —12.7  0.12 24 0.6 0.22 12 LNU332 64822.4 — — — 12.7  0.17 23 — — —LNU332 64824.3 0.8 0.28 17 — — — — — — LNU307 64959.2 — — — 12.2  0.2218 0.6 0.28 11 LNU307 64960.2 — — — 12.0  0.27 16 — — — LNU303 65043.2 —— — 12.4  0.17 21 0.6 0.28 10 LNU303 65046.1 — — — 14.0  0.02 36 — — —LNU300 65030.2 — — — 12.7  0.12 23 0.6 0.23 12 LNU300 65031.3 — — —14.4  0.01 40 0.6 0.13 15 LNU300 65033.1 — — — 13.8  0.03 34 0.6 0.24 12CONT. — 0.7 — — 10.3  — — 0.5 — — LNU517 64296.3 0.7 0.17 26 9.2 0.07 34— — — LNU517 64297.9 — — — 8.6 0.14 25 — — — LNU509 64690.3 0.7 0.17 259.5 0.03 39 — — — LNU509 64692.3 0.7 0.25 22 9.0 0.06 31 — — — LNU50964692.6 — — — 8.9 0.08 30 — — — LNU504 64455.6 0.7 0.10 28 — — — — — —LNU501 64197.1 — — — 8.1 0.15 18 — — — LNU461 64666.1 — — — 8.3 0.23 20— — — LNU461 64668.5 0.7 0.23 21 8.7 0.13 26 — — — LNU397 64375.1 — — —8.7 0.13 26 — — — LNU396 64315.13 — — — 9.8 0.02 43 0.5 0.19 16 LNU37264481.1 — — — 8.4 0.16 23 — — — LNU372 64483.3 0.7 0.15 24 9.1 0.06 32 —— — LNU372 64485.1 — — — 8.0 0.29 17 — — — LNU372 64485.3 — — — 8.1 0.3017 — — — LNU369 64386.1 — — — 8.4 0.18 22 — — — LNU369 64387.1 — — — 8.30.22 20 — — — LNU365 64712.3 0.7 0.29 18 8.2 0.24 19 — — — LNU35064674.2 0.7 0.25 21 9.1 0.06 33 0.5 0.23 15 LNU345 64333.4 — — — 8.40.21 23 — — — LNU345 64337.1 — — — 8.5 0.17 24 — — — LNU342 64036.2 0.70.29 20 8.7 0.13 26 — — — LNU313 64661.8 — — — 8.5 0.14 24 — — — LNU31364663.2 — — — 9.1 0.05 33 — — — LNU294 64657.2 — — — 8.4 0.19 22 — — —CONT. — 0.6 — — 6.9 — — 0.4 — — LNU458 63893.1 0.7 0.20 20 — — — — — —LNU419 63896.2 — — — 9.0 0.23 17 — — — LNU403 64236.3 — — — — — — 0.50.26  9 CONT. — 0.6 — — 7.7 — — 0.5 — — LNU520 64156.14 — — — — — — 0.40.25 10 LNU520 64156.7 — — — 7.8 0.25 17 0.4 0.13 14 LNU493 64190.3 0.70.17 24 — — — — — — LNU493 64191.3 — — — 7.7 0.27 16 — — — LNU48164141.1 0.7 0.26 20 — — — — — — LNU472 63920.6 — — — — — — 0.4 0.29 10LNU419 63897.4 — — — 7.9 0.24 19 — — — LNU419 63897.5 — — — — — — 0.50.04 19 LNU419 63897.6 0.7 0.20 26 8.2 0.12 24 — — — LNU343 64208.1 0.70.17 25 9.0 0.03 34 0.4 0.18 12 LNU340 64290.7 — — — — — — 0.4 0.29 10LNU328 64150.1 — — — 8.2 0.12 23 0.5 0.03 21 LNU328 64151.2 0.7 0.23 21— — — — — — LNU312 64002.2 0.7 0.20 21 7.7 0.28 16 0.4 0.22 12 LNU30564114.1 0.7 0.25 17 — — — — — — CONT. — 0.6 — — 6.7 — — 0.4 — — LNU50364203.1 — — — 10.0  0.26 20 0.5 0.28 12 LNU444 64182.3 0.8 0.28 15 — — —— — — LNU430 63936.2 — — — — — — 0.5 0.26 13 LNU366 64028.3 — — — 10.0 0.27 20 — — — LNU335 64169.2 0.9 0.12 21 — — — — — — LNU317 64093.3 0.80.28 14 — — — — — — CONT. — 0.7 — — 8.4 — — 0.5 — — LNU499 64146.11 0.80.18 15 — — — — — — LNU499 64146.12 0.8 0.22 14 — — — — — — LNU49964146.7 0.8 0.28 11 — — — — — — LNU468 63492.2 0.8 0.28 12 — — — — — —LNU468 63492.3 0.8 0.04 24 — — — — — — LNU468 63493.4 0.8 0.17 16 — — —— — — LNU467 63716.1 0.8 0.26 12 — — — — — — LNU467 63718.2 0.8 0.17 14— — — — — — LNU462 63503.1 0.8 0.10 19 — — — — — — LNU462 63503.2 0.80.23 13 — — — — — — LNU462 63505.1 0.8 0.24 12 — — — — — — LNU45564187.5 0.8 0.07 20 12.8  0.23 16 — — — LNU450 63708.6 0.8 0.20 14 — — —— — — LNU450 63709.4 0.8 0.04 23 — — — — — — LNU450 63710.2 0.8 0.13 16— — — — — — LNU425 63911.7 0.8 0.06 23 — — — 0.6 0.22  9 LNU402 63913.10.8 0.21 14 — — — — — — LNU402 63913.4 0.8 0.17 14 — — — — — — LNU40263915.1 0.9 0.03 25 — — — — — — LNU399 63944.2 0.9 0.01 28 — — — — — —LNU399 63944.6 0.8 0.15 16 — — — — — — LNU399 63946.1 0.8 0.22 15 — — —— — — LNU395 64142.5 0.8 0.23 13 — — — — — — LNU395 64142.8 0.8 0.14 16— — — — — — LNU392 63696.2 0.8 0.18 15 — — — — — — LNU392 63697.4 0.80.15 15 — — — — — — LNU392 63698.2 0.8 0.13 16 — — — — — — LNU39263700.3 0.8 0.25 13 — — — — — — LNU390 63538.1 0.8 0.05 22 — — — — — —LNU390 63539.2 0.8 0.12 16 — — — — — — LNU390 63540.9 0.8 0.14 16 — — —— — — LNU349 63989.1 0.8 0.03 23 — — — — — — LNU349 63989.5 0.8 0.24 13— — — — — — LNU349 63989.6 0.8 0.14 16 — — — — — — LNU349 63990.2 0.90.01 29 — — — — — — LNU347 63510.4 0.8 0.16 15 — — — — — — LNU32963428.2 0.8 0.28 14 — — — — — — LNU329 63429.1 0.8 0.15 16 — — — — — —LNU329 63430.3 0.8 0.20 13 — — — — — — LNU323 63420.1 0.8 0.08 19 — — —— — — LNU323 63421.2 0.8 0.08 19 — — — — — — CONT. — 0.7 — — 11.0  — —0.5 — — LNU511 65037.3 — — — — — — 0.5 0.28  9 LNU511 65040.2 — — —10.0  0.16 19 0.5 0.08 15 LNU492 64175.1 0.9 0.14 20 — — — 0.5 0.20 11LNU492 64176.4 — — — 9.8 0.29 16 — — — LNU471 64839.2 — — — 10.0  0.1619 0.5 0.06 17 LNU471 64842.1 — — — 11.0  0.03 30 0.5 0.02 21 LNU46364281.3 — — — — — — 0.5 0.27 10 LNU463 64283.4 — — — 10.1  0.13 20 0.50.05 18 LNU463 64283.5 — — — — — — 0.5 0.13 14 LNU454 64797.2 — — —10.1  0.16 20 0.5 0.22 11 LNU454 64799.2 — — — 10.1  0.15 20 0.5 0.12 13LNU454 64800.5 — — — — — — 0.5 0.14 13 LNU413 65021.4 — — — — — — 0.50.20 11 LNU413 65022.4 — — — 11.7  0.01 39 0.5 0.02 20 LNU410 64971.1 —— — — — — 0.5 0.19 11 LNU387 64808.1 — — — — — — 0.5 0.08 16 LNU38764810.4 — — — — — — 0.5 0.13 13 LNU387 64811.2 — — — — — — 0.5 0.30  9LNU382 64428.2 — — — 10.7  0.06 27 0.5 0.02 21 LNU382 64429.3 — — — — —— 0.5 0.29  9 LNU373 64830.1 0.8 0.24 14 — — — 0.5 0.19 11 LNU36164834.1 0.8 0.20 16 10.1  0.15 20 0.5 0.06 16 LNU355 65012.2 — — — 11.1 0.03 31 0.5 0.02 21 LNU355 65013.2 — — — 10.2  0.15 22 0.5 0.06 18LNU355 65014.2 — — — 10.3  0.11 23 0.5 0.15 12 LNU332 64823.1 0.9 0.1818 — — — — — — LNU307 64959.2 — — — — — — 0.5 0.12 13 LNU307 64960.1 — —— — — — 0.5 0.12 14 LNU303 65043.1 — — — — — — 0.5 0.28 10 LNU30365043.2 — — — 9.7 0.29 15 0.5 0.16 13 LNU300 65032.1 0.8 0.29 14 — — — —— — CONT. — 0.7 — — 8.4 — — 0.4 — — LNU513 63458.3 0.9 0.15 14 — — — — —— LNU512 63470.1 0.9 0.07 17 — — — — — — LNU451 63497.5 — — — 10.5  0.1423 — — — LNU357 63532.3 — — — 11.0  0.08 29 — — — LNU357 63533.8 0.90.15 15 11.1  0.06 30 0.5 0.10 16 LNU357 63534.4 — — — 10.3  0.19 21 — —— LNU351 63466.1 — — — 10.0  0.28 17 — — — LNU344 63520.4 — — — — — —0.5 0.22 12 LNU330 63438.1 — — — 10.9  0.12 27 0.5 0.18 14 LNU33063441.2 1.0 0.05 19 — — — — — — LNU319 63527.1 0.9 0.25 11 — — — — — —LNU319 63528.1 0.9 0.29 11 10.4  0.20 21 — — — LNU319 63530.1 0.9 0.1515 10.1  0.23 19 — — — LNU319 63530.3 — — — 10.2  0.22 19 — — — LNU30263378.3 — — — 11.3  0.05 33 0.6 0.07 18 LNU291 63385.1 — — — 10.5  0.1423 0.5 0.19 13 LNU291 63387.3 — — — 10.7  0.12 25 0.5 0.18 13 CONT. —0.8 — — 8.5 — — 0.5 — — Table 96. “CONT.”—Control; “Ave.”—Average; “%Incr.” = % increment; “p-val.”— p-value; L means that p-value is lessthan 0.01, p < 0.1 was considered as significant.

Although the invention has been described in conjunction with specificembodiments thereof, it is evident that many alternatives, modificationsand variations will be apparent to those skilled in the art.Accordingly, it is intended to embrace all such alternatives,modifications and variations that fall within the spirit and broad scopeof the appended claims.

All publications, patents and patent applications mentioned in thisspecification are herein incorporated in their entirety by referenceinto the specification, to the same extent as if each individualpublication, patent or patent application was specifically andindividually indicated to be incorporated herein by reference. Inaddition, citation or identification of any reference in thisapplication shall not be construed as an admission that such referenceis available as prior art to the present invention. To the extent thatsection headings are used, they should not be construed as necessarilylimiting. In addition, any priority document(s) of this applicationis/are hereby incorporated herein by reference in its/their entirety.

What is claimed is:
 1. A method of increasing nitrogen use efficiency,yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, and/or abiotic stress tolerance of a plant or reducing time toflowering or to inflorescence emergence of a plant, comprisingover-expressing within the plant a polypeptide comprising an amino acidsequence at least 80% identical to the amino acid sequence selected fromthe group consisting of SEQ ID NO: 470-655, 657-784, 2398-3401,3406-3817 and 3818, thereby increasing the nitrogen use efficiency,yield, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, and/or abiotic stress tolerance of the plant or reducing thetime to flowering or to inflorescence emergence of a plant.
 2. Themethod of claim 1, wherein said polypeptide is at least 95% identical tothe amino acid sequence selected from the group consisting of SEQ IDNOs: 470-655, 657-784, 2398-3401, and 3406-3818.
 3. The method of claim1, wherein said polypeptide is selected from the group consisting of SEQID NOs: 470-655, 657-784, 2398-3401, and 3406-3818.
 4. The method ofclaim 1, wherein said polypeptide is expressed from an exogenouspolynucleotide comprising the nucleic acid sequence selected from thegroup consisting of SEQ ID NOs: 1-186, 188-442, 444-469, 785-1934, and1939-2397.
 5. The method of claim 1, further comprising growing theplant over-expressing said polypeptide under the abiotic stress.
 6. Themethod of claim 1, wherein said abiotic stress is selected from thegroup consisting of salinity, drought, water deprivation, flood,etiolation, low temperature, high temperature, heavy metal toxicity,anaerobiosis, nutrient deficiency, nutrient excess, atmosphericpollution and UV irradiation.
 7. The method of claim 1, wherein theyield comprises seed yield or oil yield.
 8. The method of claim 1,further comprising growing the plant over-expressing said polypeptideunder nitrogen-limiting conditions.
 9. The method of claim 1, furthercomprising selecting a plant over-expressing said polypeptide for anincreased nitrogen use efficiency, yield, biomass, growth rate, vigor,oil content, fiber yield, fiber quality, and/or abiotic stress toleranceas compared to a control plant under the same growth conditions.
 10. Themethod of claim 1, further comprising selecting a plant over-expressingsaid polypeptide for a reduced time to flowering or to inflorescenceemergence as compared to a control plant under the same growthconditions.
 11. A nucleic acid construct comprising an isolatedpolynucleotide comprising a nucleic acid sequence encoding a polypeptidewhich comprises an amino acid sequence at least 80% identical to theamino acid sequence set forth in SEQ ID NO: 470-655, 657-784, 2398-3401,3406-3817 or 3818, and a heterologous promoter operably linked to saidisolated polynucleotide for directing transcription of said nucleic acidsequence in a host cell, wherein said amino acid sequence is capable ofincreasing nitrogen use efficiency, yield, biomass, growth rate, vigor,oil content, fiber yield, fiber quality, and/or abiotic stress toleranceof a plant or reducing a time to flowering or to inflorescence emergenceof a plant.
 12. The nucleic acid construct of claim 11, wherein saidamino acid sequence is at least 95% identical to the amino acid sequenceselected from the group consisting of SEQ ID NOs: 470-655, 657-784,2398-3401, and 3406-3818.
 13. The nucleic acid construct of claim 11,wherein said amino acid sequence is selected from the group consistingof SEQ ID NOs: 470-655, 657-784, 2398-3401, and 3406-3818.
 14. Thenucleic acid construct of claim 11, wherein said nucleic acid sequenceis selected from the group consisting of SEQ ID NOs: 1-186, 188-442,444-469, 785-1934, and 1939-2397.
 15. The nucleic acid construct ofclaim 11, wherein said promoter is heterologous to said isolatedpolynucleotide and/or to said host cell.
 16. A plant cell transformedwith the nucleic acid construct of claim
 11. 17. A transgenic planttransformed with the nucleic acid construct of claim
 11. 18. A method ofgrowing a crop, the method comprising seeding seeds and/or plantingplantlets of a plant transformed with the nucleic acid construct ofclaim 11, wherein the plant is derived from plants which have beentransformed with said isolated polynucleotide and which have beenselected for at least one trait selected from the group consisting of:increased nitrogen use efficiency, increased yield, increased biomass,increased growth rate, increased vigor, increased oil content, increasedfiber yield, increased fiber quality, increased abiotic stresstolerance, reduced time to flowering and reduced time to inflorescenceemergence as compared to a non-transformed plant, thereby growing thecrop.
 19. A method of selecting a transformed plant having increasednitrogen use efficiency, yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, and/or abiotic stress tolerance orreduced time to flowering or to inflorescence emergence as compared to awild type plant of the same species which is grown under the same growthconditions, the method comprising: (a) providing plants transformed withan exogenous polynucleotide encoding a polypeptide comprising an aminoacid sequence at least 80% homologous to the amino acid sequenceselected from the group consisting of SEQ ID NOs: 470-655, 657-784,2398-3401, and 3406-3818, (b) selecting from said plants of step (a) aplant having increased nitrogen use efficiency, yield, biomass, growthrate, vigor, oil content, fiber yield, fiber quality, and/or abioticstress tolerance or reduced time to flowering or to inflorescenceemergence as compared to a wild type plant of the same species which isgrown under the same growth conditions, thereby selecting the planthaving the increased nitrogen use efficiency, yield, biomass, growthrate, vigor, oil content, fiber yield, fiber quality, and/or abioticstress tolerance or reduced time to flowering or to inflorescenceemergence as compared to the wild type plant of the same species whichis grown under the same growth conditions.
 20. The method of claim 19,wherein said polypeptide is at least 95% identical to the amino acidsequence selected from the group consisting of SEQ ID NOs: 470-655,657-784, 2398-3401, and 3406-3818.